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An interface between the school and research institutes IEC Sónia Ferreira May 8 th 2015

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An interface between the school and research institutes

IEC

Sónia Ferreira May 8th 2015

IEC

Location

Universidade de Coimbra

IEC

• 3 research cores

IEC

IEC Facilities

IEC

Regional influence

Aveiro

Águeda

Anadia

Cantanhede

Souselas

Bustos

Oliveira do Bairro

• 9 schools• 6 municipalities

AveiroÁguedaOliveira do BairroAnadiaCantanhedeCoimbra

IEC

IEC intervention areas

3) Programs for children/schools/teachers

1) Programs for Adults

2) Civic and cultural intervention

Programs for children/schools/teachers

IEC

Advanced Studies

IEC

An add-on to school activities

a. Advanced Courses• at IEC• at Schoolsb. Conferences:• at IEC• at Schools• at School Librariesc. Exhibitsd. Visitse. Science Clubs at Schools

IEC

IEC Advanced Studies

Advanced Courses…

IEC

Primary school 1st to 4th grades

Basic school 5th and 6th grades

7th to 9th grades

High school 10th to 12th grades

• 10 weeks, after school

• 3 hours/week

• 10-12 students (10 students+2 teachers)

Students apply on a voluntary basis

• Lab coat (experimental courses)

• Badge/Name tag

• Documentation File (program, bibliography, protocols,

notebook and a pen) IEC

Advanced Courses Structure

Advanced Courses for High School

IEC

Where?

IEC

Advanced Courses for High School

Aveiro

Águeda

Anadia

Souselas

Bustos

Oliveira do Bairro

• IEC• 7 Schools

• 2 universities• > 10 research institutes• > 80 researchers

Advanced Courses for High School

Neurosciences Molecular Biology: gene to protein Chemistry Physics Biotechnology: advanced therapies Microbiology Cellular Physiology Biomedicine Microbiological Biotechnology Ecotoxicology Mathematics Forensic Psychology Social Sciences Visual Neurosciences

IEC

Universidade de Coimbra

• 2 universities• > 10 research institutes• > 80 researchers

Which?

Advanced Course in Molecular Biology

IEC

1) Presentation

2) Diagnosis questionnaire

3) Lecture

4) Practical applications of the concepts

5) Discussion of results

6) Final questionnaire

7) Satisfaction inquiry

8) Certificate day

Goals

• Induce dynamic experimental work;

• Prepare the students to succeed in the University.IEC

Equipment

IEC

• Extraction kit;• Thermal cycler;• Microwave;• Microcentrifuge;• Micropipettes• Rocking platform;• Vortexer;• Electrophoresis chamber;• Power supply

Program

IEC

Tema I: Impressão Digital Genética. Dá-me um cabelo, dir-te-ei quem és!Data: 04 outubro 2014 (Teórica)Sessão 1: Extração de DNA; Análise por PCR; Separação deDNA em agarose e análise informática dos resultados obtidosDocente: Ana Luísa CarvalhoData: 11 outubro 2014 (Prática)Sessão 2: Extração de DNA. Amplificação do DNA extraídopor PCR.Docente:Data: 18 outubro 2014 (Prática)Sessão 3: Separação de DNA em matriz de agarose.Utilização de ferramentas informáticas disponíveis naInternet para análise de resultados.Docente:Tema II: Todos diferentes, todos iguais. Será o tubarãoprimo da truta?Data: 25 outubro 2014 (Teórica)Sessão 4: Separação de proteínas em gel e agarose; Relaçãode filogenia com base no perfil proteico.Docente: Carlos DuarteData: 01 novembro 2014 (Prática)Sessão 5: Extração e quantificação de proteínas do leite.Docente: Ana Luísa Carvalho

Data: 08 novembro 2014 (Prática)Sessão 6: Eletroforese e transferência deproteínas para uma membrana.Docente:Data: 15 novembro 2014 (Prática)Sessão 7: Imunodeteção e estabelecimento derelações filogenéticas.Docente:Tema III: Dra ELISA diagnosticaData: 22 novembro 2014 (Teórica/Prática)Sessão 8: O que é a técnica de ELISA? Utilizaçãode técnicas de ELISA em diagnóstico. Interaçãoantigénio-anticorpo.Docente:Tema IV: Cultura de células animais e fases damitoseData: 29 novembro 2014 (Teórica/ Prática)Sessão 9: Cultura de células e Imunocitoquímica.Docente: Ana Santos CarvalhoData: 06 dezembro 2014Sessão 10: Visualização das fases da mitose aomicroscópio de fluorescência e aquisição deimagens.Docente: Ana Santos Carvalho

• 10 weeks

Example

IEC

Theme I: Genetic digital fingertips.“Give me a string of your hair, I will tell you who you are! “

Ana Luisa Carvalho Carlos Duarte

CNC, Univ. Coimbra

1) Session 1: Theory

2) Session 2: Practical

3) Session 3: Practical

Experimental steps

IEC

1) Sample collection2) DNA Extraction3) DNA Amplification: Polymerase chain reaction4) DNA separation: Electrophoresis5) DNA Staining6) Results discussion

Results

IEC

+/+ +/--/- Sample

Certificate’s day

IEC

• Certificate• Book

Impact Evaluation

IEC

Assessment of acquired knowledge

IEC

Diagnose questionnaire = Final questionnaire• 10 multiple choices questions• 5 minutes to answer• avoid random answers

**p<0.01, Mean ± S.E.M of n=5 independent courses. t-test analysis.

Number of IEC students

IEC

For the period of 2011-2015

1001 studentsHigh school studentsHigh schools teachers

81 Advanced Courses

IECHigh schools

March 2015

26-04-14 IEC

Distribution of student by courses

January 2015

Conclusions

IEC

The Advanced Courses:- Improve students’ appreciation of Experimental Science

- Increase understanding of Scientific concepts

- Contribute to more informed choices by students

- Promote new social relations between students from different schools

- Engage students in Science and improve students’ curiosity andobservation skills

Overall, increase students’ Science Capital.

IEChttp://www.educacao-e-cidadania.com

Science Fair

IEChttp://www.educacao-e-cidadania.com

Acknowledgements

SFRH / BPD / 81509 / 2011SFRH / BGCT / 52116 / 2013

IEC TeamArsélio Pato de CarvalhoNuno SantosAlexandra OliveiraMarisa CostaRichard MarquesInês RoxoSérgio Portovedro

Researchers