welcome to bio 3596! my goal is to help you learn and be ...€¦ · biology 3596 is a lab-based...

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Department of Biology

BIOLOGY3596B–GENOMICSANDBEYOND–WINTER2021

WelcometoBio3596!Mygoalistohelpyoulearnandbesuccessful!

Please, read and keep this course outline handy, because it is an official document that contains important course information.

1. GeneralCourseInformation......................................................................................................................................21.1. CourseInformation............................................................................................................................2Coursedescription..............................................................................................................................................................2ListofPrerequisites............................................................................................................................................................2Modeofdelivery................................................................................................................................................................2Technicalrequirements:.....................................................................................................................................................2

1.2. OnlineParticipationandEngagement:contributiontocommunitylearning.......................................31.3. KeySessionalDates............................................................................................................................31.4 whoarewe?.......................................................................................................................................3Instructor:Dr.AnneF.Simon..............................................................................................................................................3TeachingASSISTANTS..........................................................................................................................................................3Labsupervisor.....................................................................................................................................................................3HowtoContacttheTAsortheinstructor...........................................................................................................................3EmailPolicies+Forums.......................................................................................................................................................3

2. COURSESTRUCTURE..................................................................................................................................................42.1. Laboratoriestechniques.....................................................................................................................42.2. CourseMaterials................................................................................................................................5Labnote-BOoK....................................................................................................................................................................5LaboratoryManualANDVIDEOS.........................................................................................................................................5CourseWebsites:................................................................................................................................................................5

2.3. Course-levellearningoutcomes..........................................................................................................52.4. MethodsofEvaluation.......................................................................................................................64.1. AccommodatedEvaluations...............................................................................................................7LateSubmissionsormissedEVALUATIONS.........................................................................................................................7

4.2. Equity,Diversity&inclusion(EDI)statement......................................................................................84.3. Landacknowledgment.......................................................................................................................8

5. IMPORTANTLEGALITIES............................................................................................................................................85.1. AccommodationandAccessibility......................................................................................................8AccommodationPolicies...................................................................................................................................................8AcademicConsiderationforStudentAbsence....................................................................................................................8

5.2. AcademicPolicies...............................................................................................................................9Onlinerequirements...........................................................................................................................................................9Professionalism&Privacy:..................................................................................................................................................9Alloftheremotesynchronoussessionsforthiscoursewillberecorded...........................................................................9Scholasticoffences............................................................................................................................................................10

5.3. SupportServices...............................................................................................................................11Learning-skills....................................................................................................................................................................11emotional/mentaldistress................................................................................................................................................11Additionalsupport............................................................................................................................................................11

6. AnticipatedLectureTopics,andFirmDeadlines.......................................................................................................12

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1. GENERALCOURSEINFORMATION

1.1. COURSEINFORMATION

COURSEDESCRIPTION

Apracticalintroductiontomodernexperimentalapproachesingeneticsandmolecularbiologyasappliedtosuchtopicsasgenomics(geneidentificationandclassification),functionalgenomics(genomeexpressionprofiles)andbioinformatics(computationalgenomicanalysis).

Thisyear,thegoalofthisonlinecourseistogiveyouexperiencewithunderstandingandevaluatinggeneticslaboratorytechniques,andwritinglabreportsfollowingprimaryliteratureformats.

LISTOFPREREQUISITES

Restrictedto:studentswithaminof70%inBio2581BandBio2290F/G.ANDtoyrs3&4studentsinHSPgenetics,HSPgenetics&biochem,majororminoringenetics.

UnlessyouhaveeithertherequisitesforthiscourseorwrittenspecialpermissionfromyourDeantoenrollinit,youmayberemovedfromthiscourseanditwillbedeletedfromyourrecord.Thisdecisionmaynotbeappealed.Youwillreceivenoadjustmenttoyourfeesintheeventthatyouaredroppedfromacourseforfailingtohavethenecessaryprerequisites.

MODEOFDELIVERY

Thiscoursewillbedeliveredcompletelyonline.Virtualsessionswillbeacombinationofsynchronous(live)andasynchronous(recorded).Studentscouldbeexpectedtocompleteworkpriortoattendingsessions.Timetabledsessionswillbeusedfordiscussions,andofficehours.Belowaredetailsaboutthesessions.

Mode DatesTime

EST–EASTERNSTANDARDTIME

Frequency** Attendance

Virtualsynchronous*UsingZoominOWL

MTuWed

3:30–4:30pm–lecturecontent11am–noon-Section002–willbeupdatedbasedonpoll2–3pm-Section00311am–noon-Section0042–3pm-Section005

weekly

Attendingatleast50%ofsynchronoussessionsismandatory–buthighlyrecommendedtoattendallNOTPOSTED

Virtualasynchronous N/A 4-8hours/week weekly Yes*therecordedsynchronoussessionswillNOTbesharedonowl,forprivacyreasons,astheywillmostlybeofficehours**aschedulewillbeprovided

TECHNICALREQUIREMENTS:

Stableinternetconnection

Laptoporcomputer

Workingmicrophone

Workingwebcam

Ifyouneedassistance,youcanseeksupportontheOWLHelppage.Alternatively,youcancontacttheWesternTechnologyServicesHelpdesk:519-661-3800orext.83800.

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1.2. ONLINEPARTICIPATIONANDENGAGEMENT:CONTRIBUTIONTOCOMMUNITYLEARNING

Studentsareexpectedtoparticipateandengagewithcontentasmuchaspossible

StudentsareexpectedtouseZoomforthesynchronoussessions

Students have to participate usingVoiceThread afterwatching theweekly content, no later than 24 hours beforetheirscheduledlabsession.

Studentscanalsoparticipatebyinteractingintheforumswiththeirpeersandinstructors

Studentsareexpectedtofillintheweeklycheckliststokeeptrackoftheirtasks

Studentsareexpectedtoattendtoaminimumof50%oftheTA-ledlabsessionsattheirscheduledtime.

1.3. KEYSESSIONALDATES

Classesbegin:January11ReadingWeek:February13-21Classesend:April12

1.4 WHOAREWE?

INSTRUCTOR:DR.ANNEF.SIMON(She/Her)Workingfromhomethisyear-Email:asimon28@uwo.ca

TEACHINGASSISTANTSCarlyCharon(She/Her)ccharro5@uwo.ca Section002

JoshIsaacson(He/Him)jisaacso@uwo.ca Section003

OliviaSiemons(She/Her)osiemons@uwo.ca Section004

WesRobinson(He/Him)jrobi8@uwo.ca Section005

LABSUPERVISOR

Ms.KimLoney(She/Her)kgrant4@uwo.ca

HOWTOCONTACTTHETASORTHEINSTRUCTOR

IencourageyoutocometotheTAsormyselfwithquestionsand/orcomments.Thiscanbedonebyappointment,orduringin-personsessions,orfinallybye-mail(seedetailsbelowandonowl),orbypostingonthediscussionboardsonOwl(yourpeersmayalsobeabletoansweryourquestion).Pleasedonotleavemephonemessages.

EMAILPOLICIES+FORUMS

Emailhours:Weusuallycheckemailsonceaday,exceptforweekendsandholidays.Wetrytoanswerwithin24hrs,withthefollowinglimitations:

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Yourinstructor’sandTAs’emailsshouldonlybeusedforadministrativepurposes.• Todiscussgradesoraccommodation,pleasesendanemailtomakeanappointment• IfyouemailyourinstructororTAs,youmustuseyourWesternemailaddressandincludeBio3596inthesubjectline.

Messagesfromanon-WesternaccountorthosethatdonotincludeBio3596maybeblockedbytheuniversity’santi-spamsystem.

• Emailsfromnon-UWOaddressesorwithoutBio3596inthesubjectlinewillbedeleted,aswillemailsthatareimpoliteorwritteninthemannerofatextmessage.

InordertomaximizeefficiencyandtoallowyourinstructorandTAstorespondtolegitimateconcernsasquicklyaspossible,usetheforumorattendsynchronoussessionsforissuesofthefollowingnature:

• Questionsaboutcoursematerialoronhowtoprepareforlaboratoriesorassignments.SuchquestionsshouldbetakentothesynchronoustimeonMondays,orthelabsectionssynchronoustimes,astheyaretypicallyansweredfarbetterandquickerinperson.

Avoidthefollowingtypeofquestions,theywillnotbeansweredbytheteachingteam:• Questionsthatcanbeansweredbasedontheinformationfoundinthiscourseoutline.Beingabletofindinformationyourselfisanimportantsoft-skillandanemployabilityoutcome.

2. COURSESTRUCTURE

Biology3596isalab-basedcoursethatmeetstwiceperweek:oncefor1-hourconcept-baseddiscussionsandagainfora1-hourtechnicallabdiscussionduringthetimespecifiedforeachsection.Studentsareexpectedtoreadthelabmanualinadvance,andarrivetodiscussionsfullyinformedabouttheweek’sexperiments.

Thecourseisdividedintofivemainexperiments.Eachmainexperimentcontainsaseriesofstepsthattypicallyrunoverseveralweeks.Thisyear,eachexperimentwillbepresentedindependently,over2weeks.Attheendofeachmainexperimentareportsummarizingandintegratingtheresultsandinterpretationofexperimentsfromthatmainexperimentwillbedue.

Youareexpectedtoattendatleast50%ofallthesynchronoussessions.Additionalabsences,withorwithoutjustification,willleadtodeductionstowardyourparticipationgrade.

Thiscourseischallengingandfastpaced.Tobesuccessful,youmustengage,readtheassignedmaterialandperformtherequiredtasks.Donotfallbehind!Usethecheckliststohelpinyourworkorganization.

Ifafteryoustudy,youdonotunderstandsomething,pleaseseetheinstructororTAsforclarification,ideallyduringthesynchronoussessions,oraskquestionsontheappropriateowlforumsectionforfasteranswersbyyourpeers.

TheOWLweeklycontent,readingsandvideos,willprovidebackgroundinformationonlaboratorytechniques.Thereisnotextinthiscourseandnoteswillnotbedistributed.Althoughsomelecturematerialwillbemadeavailable,thismaterialcannotbeconsideredtorepresentlecturenotes.Therefore,itisimperativethatstudentsattendalllecturesandconstructtheirownsetofnotes.

Itisyourresponsibilitytogatherandreviewtheinformationandtoaskquestions.

2.1. LABORATORIESTECHNIQUES

Youwilllearnthetheory,andbeabletounderstandthroughvideodemonstrations,howtoperformaseriesofGeneticandMolecularBiologyTechniques:

PolymeraseChainReaction(PCR)RestrictionEnzymeDigestionAgaroseGelElectrophoresisPolyacrylamideGelElectrophoresis(PAGE)

DNAextraction

CloningRNAisolationReverseTranscription(RT-PCR)YeastGeneticsGeneticComplementation

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2.2. COURSEMATERIALS

LABNOTE-BOOK

YOUNEED:anaccountonBenchling.com(free).Anexplanatoryvideocanbefoundonowl:

LABORATORYMANUALANDVIDEOS

AlaboratorymanualoutliningallexperimentsisavailablethroughtheBiology3596OWLwebsite(https://owl.uwo.ca/x/Rrrpx7),inpdfformat.Studentsareexpectedtoreadthelabsdescriptionandprotocolsinadvanceandbereadywhenlabstarts.Ahardcopyoftheproceduresisrequiredtobepastedinthelabnotebook.

Inaddition,therearevideosdemonstratingthelabtechniques,accessibleweeklyontheowlwebsite.

COURSEWEBSITES:

AllcoursematerialwillbepostedtoOWL:https://owl.uwo.ca/x/Rrrpx7.

If students need assistance, they can seek support on theOWLHelp page. Alternatively, they can contact theWesternTechnologyServicesHelpdesk.Theycanbecontactedbyphoneat519-661-3800orext.83800.

Google Chrome orMozilla Firefox are the preferred browsers to optimally use OWL; update your browsers frequently.StudentsinterestedinevaluatingtheirInternetspeed,pleaseclickhere.

StudentsshouldcheckOWL(http://owl.uwo.ca)onaregularbasisfornewsandupdates.Thisistheprimarymethodbywhichinformationwillbedisseminatedtoallstudentsintheclass.ThemissingofcriticalinformationduetoyourfailuretocheckOWLcannotbeusedasabasisforappeal.AnnouncementsaboutthecoursewillbesenttoyourUWOemailaddress.

Itisyourresponsibilitytokeepthisaccountinastatethatallowsyoutoreceiveemail,andtocheckitregularly.Asabove,afailuretocheckyourUWOemailcannotbeusedasabasisforappeal.

2.3. COURSE-LEVELLEARNINGOUTCOMES

COURSEOBJECTIVES SpecificLearningOutcomes

Studentswill:AssessmentMethods

Independentlearning

• Accessmaterialontheirown:o Readingtheresourceso Protocolsandprimaryscientificliteratureo Videos

• Assesstheirunderstandingindependently

QuizzesVoiceThreadsForumsWeeklymeetings

LearnMolecularBiologyandGenetictechnicalbenchskills

Learnthetheory,andbeabletounderstandthroughvideodemonstrations,howtoperformaseriesofGeneticandMolecularBiologywetbenchTechniques

QuizzesLabReports

LearnbasicDNAanalysisskillsLearnthetheory,andbeabletounderstandthroughavideodemonstration,aswellasthroughtheirownrumblingssomebasicwayshowtoanalyseaDNAsequence

ESTsequenceanalysisandreport

Learntosetupprotocols Calculatetheproperconcentrationsandvolumesnecessarytoperformenzymaticexperiments Quizzes

Recorddata Allowsomeoneelsetoreproducetheexperimentsperformed Labnotebook

Evaluateresults Troubleshoot,Interpretrealresultsobtainedandassignedrandomlythroughthesemester

LabnotebookLabreports

Reportyourlabresults Beabletowritelabreportsintheformatofaresearcharticle Labreports

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4%9%

12%

15%

15%10%

10%

25%

5quizzeseachworth5%

Assignment2:Exp1–MurderMystery

ESTanalysis

Labnotebook

Introlabreport

2.4. METHODSOFEVALUATION

Markswillnotbe“rounded”or“curved”.Yourgradeisyourgrade.DetailsofhoweachassignmentisscoredwillbepostedonOWL.

1. LabpreparationQuizzes:5quizzesfor5%each! 25%ofyourgrade.5quizzestobetakenbeforeeachofthe5labexperimentsvideosarevisible(pre-requisitetoaccesslabvideos).Youcanbeexcusedfor2,needtohavetaken3topassthisclass.2. Laboratorynotebook! 10%ofyourgrade.Youwill need to get a lab notebook thatwill contain your protocols, and lab results (as explained in the lab notebookguidelinesonowl).Thegradewillbebasedonyourabilitytokeeptrackandproperlylabeltheresults,thequalityofyourpresentationandorganization,andfigurelabeling.Youneedtosubmityourlabnotebookforevaluationtopassthisclass.ONLINEbenchling3. LabReports:5reportswithincrementalweightfrom4-15%! 55%ofyourgrade.YouNEEDtosubmitALLoffiveassignmentstopasstheclass.Puttheexperiment intocontext–evaluateresults–explaintrouble-shooting– interpretyour labresultsforeachofthefiveexperiments.Themarksforeachassignmentareincreasingthroughouttheterm,basedonlengthanddifficultyoftheexperimentandyourincreasedexperience.Assignments will be submitted on OWL through Turnitin for plagiarism evaluation, and graded on Gradescope(www.gradescope.com).4. ESTanalysis:10%ofyourgrade.Youneedtosubmitthisanalysistopassthisclass.YouwillbegivenauniqueESTsequencetoanalyze.Detailswillbeprovidedinclassandonline.ESTanalysiswillbesubmittedonOWLthroughTurnitinforplagiarismevaluation.

Exp2–Geneexpressionlabreport

Exp4–Yeastcomplementationlabreport

Exp3–Cloninglabreport

Exp1–MurderMysterylabreport

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Contrarilytotheotherrubrics,inwhichyouneedtoearnpoints,hereweareassuminggoodintentions,andupto10%ofyourcoursegradecanlostforpoorperformance.

Assumes: respectful forum,at least50%attendance to synchronous sessions (1%permissed session,after50%of themwereattendedAND5%coursegradelostpereventsdisplayinglackofrespectyourpeersor instructors/TA),1%lostperlackofVoiceThreadcontribution.

Exception:recurrentdisrespectfuleventsmightleadtomorethana10%lossincoursegrade.

Allmarkswill be posted regularly to the classOWLwebsite.All appealsmust be submitted inwriting to the instructorwithintwoweeksofthemarkbeingpostedwithaclearexplanationofthereasonfortheappeal.Marksforthelabnotebookwritteninpencilmaynotbeappealed.Theinstructormayre-gradeallorpartoftheexamorassignmenttolookforadditionalerrorswhichmaylowerorraisethefinalmark.

Click here for a detailed and comprehensive set of policies and regulations concerning examinations and grading. ThetablebelowoutlinestheUniversity-widegradedescriptors.

A+ 90-100 OnecouldscarcelyexpectbetterfromastudentatthislevelA 80-89 SuperiorworkwhichisclearlyaboveaverageB 70-79 Goodwork,meetingallrequirements,andeminentlysatisfactory

EXPECTEDFROMYOUC 60-69 Competentwork,meetingrequirementsD 50-59 Fairwork,minimallyacceptableF below50 Fail

4.1. ACCOMMODATEDEVALUATIONS

LATESUBMISSIONSORMISSEDEVALUATIONS

Allassignmentsaredueat11:55pmEasternTimeunlessotherwisespecified

If you choose to submit your assignment late, without a self-reported absence (SRA) or an approved request foraccommodation,youalsochoosetolose-20%perlateday

Lateassessmentswithself-reportedabsencesshouldbesubmittedwithin24hoursoftheendofthe48-hourperiod.

Essentialrequirement:youneedtosubmitALLoftheassignmentstopasstheclass,evenafter5latedays(andagradeof 0 for that assignment). Failure to pass or non-completion of the assignments component of the course is anautomaticfailureofthecourse

Missedquizzeswithoutself-reportedabsences(SRA)cannotbeaccommodated.Studentswillreceive0%(outof5%)foreachmissedquiz.

Missedquizzeswithself-reportedabsences(SRA)orapprovedrequestforacademicconsiderationwillbeaccommodated.Theweightofeachmissedquiz(5%)willbetransferredtotheweightoftheoverallquizweight(e.g.,onemissedquizmeansthattheweightoftheeachquizzeswillstillbe25%,buteachquiz’sweightwillshiftfrom5%to6.25%).However,studentsmusthavecompletedatleast3ofthe5quizzestopassthecourse.

Studentswhomissmorethan6Mondaysessions,ormorethan6labsessions,withorwithoutaself-reportedabsence(SRA)willreceive1%coursegradepenaltyforeachmissedsession.

Missedsynchronoussessionscannotberescheduled,andwillnotbeposted.

Alsoseesection5.1onACADEMICCONSIDERATIONFORSTUDENTABSENCE.

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4.2. EQUITY,DIVERSITY&INCLUSION(EDI)STATEMENT

InkeepingwithoursharedvaluesandbasedontheprinciplesofdiversityandequitydefinedinthepoliciesofWesternUniversity(http://www.uwo.ca/hr/diversity/index.html)wehaveadoptedadefinitionofdiversitythatincludesallfacetsandanydifferencesthatdefinehowindividualsperceivethemselves.

4.3. LANDACKNOWLEDGMENT

WeacknowledgethatWesternUniversityislocatedonthetraditionallandsoftheAnishinaabek,Haudenosaunee,LūnaapéewakandAttawandaronpeoples,onlandsconnectedwiththeLondonTownshipandSombraTreatiesof1796andtheDishwithOneSpoonCovenantWampum.ThislandcontinuestobehometodiverseIndigenouspeoples(e.g.FirstNations,MétisandInuit)whomwerecognizeascontemporarystewardsofthelandandvitalcontributorsofoursociety.

MoreinformationaboutIndigenousServices(https://indigenous.uwo.ca/)andthisLandAcknowledgement(https://communications.uwo.ca/comms/land-acknowledgement/)areavailable.

5. IMPORTANTLEGALITIES

5.1. ACCOMMODATIONANDACCESSIBILITY

ACCOMMODATIONPOLICIES

StudentswithdisabilitiesworkwithAccessibleEducation(formerlySSD)whichprovidesrecommendationsforaccommodation basedonmedicaldocumentation orpsychological andcognitivetesting.TheAcademicAccommodationforStudentswithDisabilitiespolicycanbefoundat:https://www.uwo.ca/univsec/pdf/academic_policies/appeals/AcademicAccommodation_disabilities.pdf

PleasevisittheScience&BasicMedicalSciencesAcademicCounsellingwebpageforinformationonadd/dropcourses,academicconsiderationsforabsences,appeals,examconflicts,andmanyotheracademicrelatedmatters:https://www.uwo.ca/sci/counselling/ForpolicyonAcademicConsiderationforStudentAbsences-UndergraduateStudentsinFirstEntryPrograms,see:https://www.uwo.ca/univsec/pdf/academic_policies/appeals/Academic_Consideration_for_absences.pdfandfortheStudentMedicalCertificate(SMC),see:http://www.uwo.ca/univsec/pdf/academic_policies/appeals/medicalform.pdf

ACADEMICCONSIDERATIONFORSTUDENTABSENCE

Self-ReportedAbsence(SRA):Studentswillhaveuptotwo(2)opportunitiesduringtheregularacademicyeartouseanon-lineportaltoself-reportanabsenceduringthesemester,providedthefollowingconditionsaremet:theabsenceisnomorethan48hoursinduration,andtheassessmentforwhichconsiderationisbeingsoughtisworth30%orlessofthestudent’sfinalgrade.Studentsareexpectedtocontacttheirinstructorswithin24hoursoftheendoftheperiodoftheself-reportedabsence,unlessnotedonthesyllabus.

Studentsarenotabletousetheself-reportingoptioninthefollowingcircumstances:• forexamsscheduledbytheOfficeoftheRegistrar(e.g.,DecemberandAprilexams)• absenceofadurationgreaterthan48hours,• assessmentsworthmorethan30%ofthestudent’sfinalgrade,• ifastudenthasalreadyusedtheself-reportingportaltwiceduringtheacademicyear

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IftheconditionsforaSelf-ReportedAbsencearenotmet,studentswillneedtoprovideaStudentMedicalCertificateiftheabsenceismedical,orprovideappropriatedocumentationiftherearecompassionategroundsfortheabsenceinquestion.StudentsareencouragedtocontacttheirFacultyacademiccounsellingofficetoobtainmoreinformationabouttherelevantdocumentation.

Studentsshouldalsonotethatindividualinstructorsarenotpermittedtoreceivedocumentationdirectlyfromastudent,whetherinsupportofanapplicationforconsiderationonmedicalgrounds,orforotherreasons.AlldocumentationrequiredforabsencesthatarenotcoveredbytheSelf-ReportedAbsencePolicymustbesubmittedtotheAcademicCounsellingofficeofastudent'sHomeFaculty.

5.2. ACADEMICPOLICIES

Inaccordancewithpolicy,http://www.uwo.ca/its/identity/activatenonstudent.html,thecentrallyadministerede-mailaccountprovidedtostudentswillbeconsideredtheindividual’sofficialuniversitye-mailaddress.Itistheresponsibilityoftheaccountholdertoensurethate-mailreceivedfromtheUniversityathis/herofficialuniversityaddressisattendedtoinatimelymanner.

ONLINEREQUIREMENTSCompletionofthiscoursewillrequireyoutohaveareliableinternetconnectionandadevicethatmeetsthesystemrequirementsforZoom.Itisyourresponsibility,asastudenttoensurethatyoursystemisworkingproperly.Informationaboutthesystemrequirementsareavailableatthefollowinglinks:https://support.zoom.us/hc/en-us

ZoombestPractices:https://wts.uwo.ca/zoom/best_practices/index.html

PleasenotethatZoomserversarelocatedoutsideCanada.IfyouwouldprefertouseonlyyourfirstnameoranicknametologintoZoom,pleaseprovidethisinformationtotheinstructorinadvanceofthesession.

PROFESSIONALISM&PRIVACY:

Western students are expected to follow the Student Code of Conduct. Additionally, the following expectations andprofessionalconductapplytothiscourse:

StudentsareexpectedtofollowonlineetiquetteexpectationsprovidedonOWL

Allcoursematerialscreatedbytheinstructor(s)arecopyrightedandcannotbesold/shared

Recordingsarenotpermitted(audioorvideo)withoutexplicitpermission

Permittedrecordingsarenottobedistributed

Studentswillbeexpectedtotakeanacademicintegritypledgebeforesomeassessments

Allrecordedsessionswillbedisposedwithinoneweek–ifnoincidenttookplace

ALLOFTHEREMOTESYNCHRONOUSSESSIONSFORTHISCOURSEWILLBERECORDED.

Synchronoussessions:synchronoussessionsinthiscoursewillbeconductedusingZoom.Youmightberequiredtoturnyourcameraonatsomepointduringtheentiresession;yourmicrophoneshouldbemuteduntilitisyourturntospeak.Thesessionswillberecorded*,forevaluationpurposes,butnotposted.OnlyparticipantsusingtheirUWOcredentialswillbepermittedtoaccessthesessions.

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Netiquette:Thesynchronouscomponentofthiscoursewillinvolveonlineinteractions.Toensurethebestexperienceforbothyouandyourclassmates,pleasehonourthefollowingrulesofetiquette:

1. please“arrive”tothesessionsontime2. pleaseuseyourcomputerand/orlaptopifpossible(asopposedtoacellphoneortablet)3. ensurethatyouareinaprivatelocationtoprotecttheconfidentialityofdiscussionsintheeventthataclassdiscussion

dealswithsensitiveorpersonalmaterial4. tominimizebackgroundnoise,kindlymuteyourmicrophonefortheentireclassuntilyouareinvitedtospeak,unless

directedotherwise5. pleasebepreparedtoturnyourvideocameraoffattheTA’srequestiftheinternetconnectionbecomesunstable6. unlessinvitedbyyourTA,donotshareyourcomputerscreeninthemeeting.TheTAswillactasmoderatorforthe

classandwilldealwithanyquestionsfromparticipants.Toparticipatepleaseconsiderthefollowing:• ifyouwishtospeak,usethe“raisehand”functionandwaitfortheTAtoacknowledgeyoubeforebeginningyour

commentorquestion• remembertounmuteyourmicrophonebeforespeaking• self-identifywhenspeaking.• remembertomuteyourmicafterspeaking(unlessdirectedotherwise)

Generalconsiderationsof“netiquette”:

• keepinmindthedifferentculturalandlinguisticbackgroundsofthestudentsinthecourse.• becourteoustowardtheTA,yourcolleagues,andauthorswhoseworkyouarediscussing.• berespectfulofthediversityofviewpointsthatyouwillencounterintheclassandinyourreadings.Theexchange

ofdiverseideasandopinionsispartofthescholarlyenvironment.“Flaming”isneverappropriate.• beprofessionalandscholarlyinallonlinepostings.Citetheideasofothersappropriately.

Notethatdisruptivebehaviourofanytypeduringonlineclasses,includinginappropriateuseofthechatfunction,isunacceptable.StudentsfoundguiltyofZoom-bombingaclassorofotherseriousonlineoffensesmaybesubjecttodisciplinarymeasuresundertheCodeofStudentConduct.

Recording:Participantsinthiscoursearenotpermittedtorecordthetutorialsessions,exceptwhererecordingisanapprovedaccommodationandtheparticipanthasthepriorwrittenpermissionoftheinstructorandTA.

Generaldiscussionforum:Studentsareencouragedtopostcourse-relatedquestionsinatimelymanner,thesoonerthebetter.StudentscannotexpecttheirTAtoanswerquiz-relatedquestionsinlessthan24hrs.Studentsareencouragedtohelponeanotherbypostinganswerstootherstudents’questions.

*Thislinkprovidesanexcellentoverviewofgood’Netiquette’:https://coursedesign.colostate.edu/obj/corerulesnet.html

SCHOLASTICOFFENCES

Scholasticoffencesaretakenseriouslyandstudentsaredirectedtoreadtheappropriatepolicy,specifically,thedefinitionofwhatconstitutesaScholasticOffence,atthefollowingWebsite:http://www.uwo.ca/univsec/pdf/academic_policies/appeals/scholastic_discipline_undergrad.pdf.

ReviewBiology2290learningoutcomes.Youareexpectedtoknowwhatplagiarismisatthisstageofyourprogramme.

Turnitinaidsinidentifyingplagiarism.AllrequiredpapersandquizzesmaybesubjecttosubmissionfortextualsimilarityreviewtothecommercialplagiarismdetectionsoftwareunderlicensetotheUniversityforthedetectionofplagiarism.Allpaperssubmittedforsuchcheckingwillbeincludedassourcedocumentsinthereferencedatabaseforthepurposeofdetectingplagiarismofpaperssubsequentlysubmittedtothesystem.Useoftheserviceissubjecttothelicensingagreement,currentlybetweenTheUniversityofWesternOntarioandTurnitin.com(http://www.turnitin.com).

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5.3. SUPPORTSERVICES

Pleasecontactthecourseinstructorifyourequirelectureorprintedmaterialinanalternateformatorifanyotherarrangementscanmakethiscoursemoreaccessibletoyou.YoumayalsowishtocontactStudentAccessibilityServices(SAS)at661-2147ifyouhaveanyquestionsregardingaccommodations.

ThepolicyonAccommodationforStudentswithDisabilitiescanbefoundhere:https://www.uwo.ca/univsec/pdf/academic_policies/appeals/Academic%20Accommodation_disabilities.pdf

LEARNING-SKILLS

Learning-skillscounsellorsattheStudentDevelopmentCentre(http://www.sdc.uwo.ca)arereadytohelpyouimproveyourlearningskills.Theyofferpresentationsonstrategiesforimprovingtimemanagement,multiple-choiceexampreparation/writing,textbookreading,andmore.IndividualsupportisofferedthroughouttheFall/Wintertermsinthedrop-inLearningHelpCentre,andyear-roundthroughindividualcounselling.

EMOTIONAL/MENTALDISTRESS

Studentswhoareinemotional/mentaldistressshouldrefertoMentalHealth@Western(http://www.health.uwo.ca/mental_health)foracompletelistofoptionsabouthowtoobtainhelp.

WesternUniversityiscommittedtoathrivingcampusaswedeliverourcoursesinthemixedmodelofbothvirtualandface-to-faceformats.WeencourageyoutocheckouttheDigitalStudentExperiencewebsitetomanageyouracademicsandwell-being:https://www.uwo.ca/se/digital/.

ADDITIONALSUPPORTAdditionalstudent-runsupportservicesareofferedbytheUSC,http://westernusc.ca/services.

ThewebsiteforRegistrarialServicesishttp://www.registrar.uwo.ca.

ThefollowinglinksprovideinformationaboutsupportservicesatWesternUniversity:

AppealProceduresStudentDevelopmentServicesStudentHealthServices

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6. ANTICIPATEDLECTURETOPICS,ANDFIRMDEADLINES

Week#Date Content Assignments Lab

NovelExperimental

technics

1/Jan11 Introduction QuizEntryTest Introductiontolabs

Do’sanddon’tLabsafety

2/Jan18

How/whentodomostcommontechniques?

PCR,RE,agarose,gelimaging,CRISPR

Quizplagiarism IntrolabPipettetestPCR,DigestAgaroseGel

3/Jan25

Howtodoforensicgenotyping?

(DNAextraction,Polymorphicmarkers,PAGEgels)

Quizintrolab Exp1:Murdermystery

SwabDNAextraction

4/Feb1 DiscussIntrolabassignment Introlabreport Exp1:Murdermystery PAGE

5/Feb8WhatareESTs?

Whereisthesequencefrom?ESTassignment

QuizExp1 ESTsequencereceived

FlybaseBLASTOMIM

Feb15 READINGWEEK

6/Feb22

Research:Usingmodelsystems

Light-sensitivepathwayinArabidopsis

Exp1report Exp2:Geneexpression

RNAextractionPlantpictures

7/March1

Howtellgeneexpression?Phenotypeduetomutationin

light-responsegenes?DiscussExp1report

ESTsequenceanalysis

Exp2:Geneexpression

cDNAsynthesisRT-PCR

8/March8How/whytodocloning?

(plasmids,vectors,cloning,etc.)

QuizExp2 Exp3:Cloning AgaroseIsolationLigation

9/March15 CloningcontinuedDiscussESTassignment Exp2report Exp3:Cloning Transformation

MiniprepDNA

10/March22

Howcanwetellifamutationis

inaknowngene?Yeastexperiment,genetic

screens

QuizExp3 Exp4:yeastPickandre-streak

colonyPickacolony

11/March29 Yeastcomplementation Exp3report Exp4:yeast

YeastmatingComplementation

analysisgalactosidase

assay

12/April5

Guestpresentationanddiscussionpanel:

PatriciaMasonandLaurenStarr“careersinscience”

QuizExp4

April12-17 Exp4report