tilv pathology and histopathology€¦ · nicholson and surachetpong et al., 2019 under review ......

10/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

UTF/077/ZAM: Technical

Assistance to the Zambia

Aquaculture Enterprise

Development Project (ZAEDP)

TiLV Pathology and Histopathology

Win Surachetpongfvetwsp@ku.ac.th

Kathy Tang-Nelson

ktangnelson@gmail.com

Sample collection for diagnostic proceduresOrgans for TiLV diagnosis

Mucus

LiverSpleen

Head kidneyBrain

Meat

Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019

310/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Source: http://tupac.tue-image.nl/node/2

410/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Normal

Infected

Gross pathology

Surachetpong et al., 2017 Emerging Infectious Diseases

510/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Gross pathology

Nicholson and Surachetpong et al., 2019 Under review

• Ascites• Pale liver/gills• Corneal opacity

Brain show redness

Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019

Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019

810/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Thangaraj et al., 2018 Aquaculture

• Syncytial cells• Increase sinusoidal spaces

• Ascitic fluid, pale liver• Corneal opacity

910/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Normal TiLV-infected

Win Surachetpong

1010/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Normal TiLV challengedAnteriorkidney

Spleen

• Increase melanomacrophage

• Foamy cells

• Increase melanomacrophage

• Foamy cells, enlarge nucleus

• Loss of Red blood cells

• Accumulation of proteinacious

material

Tattiyapong et al., 2017 Vet Microbiology

Histopathology (level II)

11

Brain

Liver

Normal Infected

Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019 Tattiyapong et al., 2017 Vet Microbiology

1210/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

Surachetpong et al., 2017 Emerging Infectious Diseases

Brain

Liver

1310/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Jansen et al., 2018 Rev Aqua

• Foamy cytoplasm

• Syncytial cells

• Intracytoplasmicinclusion body

1410/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Jansen et al., 2018 Rev Aqua

• Syncytial cells

• Severe infiltration of lymphocytes

• inflammation

H&E histology of eye lens in TiLV-infected tilapia. (A) Cataractous changes characterized by formation of

eosinophilic spherical structures accompanied by degeneration of crystalline fibers (encircled); (B) control

lens from healthy fish.

A B

Histopathological changes in eye

Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019

Thank you for your attention

10/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics

UTF/077/ZAM: Technical

Assistance to the Zambia

Aquaculture Enterprise

Development Project (ZAEDP)