solexa tag analysis
Post on 01-Feb-2016
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Solexa tag analysis
0620
ahsan
今日の結果• 発現量の比較結果
– Upload 済み• 遺伝子の名前で keyword 検索できる• Distance20 かつ tag40 以上 cluster を作
りました。– Cluster の代表 TSS 決まる core
promoter 領域の motif 解析– Core promoter motif の module の存在– Annotation TSS から代表 TSS の距離– Annotation TSS から cluster 開始点の距離
Tag to Gene Relation
Keyword 検索
URL: http://dev.utgenome.org/~utgb/drosophila/genome_browser/browser.php
Keyword: 遺伝子名前を記入するKeykinds: geneName を選択する
FBtr0083733 sqz-RAFBtr0084012 ppan-RAFBtr0078728 Xe7-RAFBtr0078729 Xe7-RBFBtr0081642 Aly-RAFBtr0076616 Gug-RA
FlybaseTrID GeneName
DNA Replication Element Binding Factor
Image1 :Core promoter. Transcription Start Site (TSS) position is 0, where TATA at -30 to -25 , DPE at +29 to +33, MTE at downstream. DRE position is not restricted by upstream of TSS.
DRE TATA Inr DPE
Initiator Downstream Promoter Element
TATA Box
MTE
Motif Ten Element
Core promoter Structure
MotifChromosom
alCore
promoter
DRE 23,792 527
TATA 31,629 715
INR 52,111 371
DPE 29,781 455
MTE 45,196 547
motif element in Drosophila Chromosome and RepTSS core promoter
TATA motif position in ResTSS core promoter
INR motif position in ResTSS core promoter B
DPE motif position in ResTSS core promoter C
MTE motif position in ResTSS core promoter D
(TATA,INR,DPE,MTE) RepTSS #
0000 18,600
0001 505
0010 403
0100 320
1000 652
1100 22
1010 21
1001 18
0110 18
0101 10
0011 12
0111 0
1011 1
1110 0
1101 1
1111 0
co-existence of binding motif in core promoter region
Here 0,1 express, the present and absent of each motif elements in written order
TSS distance
Larva(distance from TSS to RepTSS)
0
200
400
600
800
1000
-1500 -1000 -500 0 500 1000 1500
distance
num
ber
of T
rans
crip
t
AllTag
0
1000
2000
3000
4000
5000
6000
-1000 -500 0 500 1000 1500 2000 2500 3000
cluster StartPoint distance from annotationTSSTr
ansc
riptio
n N
umbe
r
Annotation TSS から代表 TSS の距離 Annotation TSS から Cluster の開始点の距離
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