rise afnnet fellows conference 15 th feb.2012,kampala uganda
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RISE AFNNET FELLOWS CONFERENCE 15TH FEB.2012,KAMPALA UGANDA.
Dr.Ochwang'i Dominic,
Department of Veterinary Anatomy&Physiology,
Faculty of Veterinary Medicine,
University of Nairobi, Kenya
Project title
IN-VITRO PHARMACOPHYSIOLOGICAL ANTI-CANCER POTENTIAL OF MEDICINAL PLANTS USED IN KAKAMEGA COUNTY,KENYA.
University Supervisors
Dr. Charles Kimwele, PhD
Dept. Of Vet.Anatomy and PhysiologyDr.Jemimah Oduma,PhD
Dept. Of Vet.Anatomy and PhysiologyProf.P.K.Gathumbi,PhD
Dept. Of Vet.Pathology,Parasitology and Microbiology
Prof.Stephen G. Kiama, PhD
Dept. Of Vet.Anatomy and Physiology.
Anand et al.,2008
Hallmarks of Cancer
Account for 13% of all deaths yearly(invasive cancer),7.6 million people died; most common:
Lung cancer 1.4 million deathsStomach cancer 74,00 deathsLiver cancer 700,000 deathsColorectal cancer 610,000 deathsBreast cancer 460,000 deathsHalf of the cases in the developing world50 Kenyans die daily from various cancers,80,000 cases
diagnosed each year.
Epidemiology of cancer
WHO,2010,Jemal A,et al 2011
Holland and James,2007,Vickers A ,2004
Current treatment regimes
Typhonium flagelliforme (Mohan et al, 2008)Murraya koenigii(Syam et al 2011) Catharanthus roseus which yields vinblastine
and vincristine(Lucy and Edgar 1999)Vitex doniana, Flueggea virosa,
Ovariodendron anisatum, Launea cornuta, Grewia villosa, Maytenus obscura and Prunus africana(Kareru et al, 2007).
Identify and document anti-cancer medicinal plants used in Kakamega County, Kenya
Objective of study
Justification
Vicker,2004
Methodology
Butere, Mumias, Matungu, Khwisero, Shinyalu, Lurambi, ikolomani, Lugari and Malava
Study area
Data collection
Data collection32 consenting traditional herbal medical practitioners were interviewed.Herbalists were randomly selected from
Kakamega county.
Results 66 plants belonging to 59 genera and 32
families were cited.Most commonly cited plant species were
Spathodea campanulata P.Beauv.ssp.nilotica(Seem), Microglossa pyrifolia (Lam.) Kuntze, Harungana madagascariensis Lam.ex poir, Prunus africana(Hook.f.)kalkman, Cyphostemma serpens(A.Rich) and Aloe spp.
Commonest plant families documented for treatment of cancer (Table 1a)
Commonest plant families documented for treatment of cancer (Table 1b)
ConclusionsThe findings of study show that a variety of
medicinal plants are used in management and treatment of cancer .
The main mode of administration is oral.Many medicinal plants are taken as a
concoction.
Pending workExtract preparationBrine shrimp lethality test9
Meyer et al 1982,Nguta et al 2011)
Invitro physiological tests using human cell lines
MTT assay(Ghavami et al 2010)
Antifungal MIC Test (Ghavami et al, 2010)
Hemolytic Assay (Ghavami et al 2010, Bock and Muller,1994)
Apoptosis induction assays(Machana et al,2011) which include:
a)Nuclear staining with DAPI fluorescent dye
b) DNA fragmentation detection assay
c) Alkylating activity assayPhytochemistry.
Acknowledgement: Carnegie Corporation of New York Regional Initiative in Science and Education- African Natural Product Training Network(RISE-AFNNET) for funding.Mr.Mutiso,Mr.Mugweru, Kakamega herbalists and my supervisors.
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