measurement of cannabinoid and terpene profiles in

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Measurement of Cannabinoid and Terpene

Profiles in Cannabis Extracts by Mid-Infrared

Spectroscopy

Brian C. Smith, Ph.D.

Chief Technical Officer

Big Sur Scientific

Brian@bigsurscientific.com

(508) 579-6514

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Outline

I. Does the Cannabis Industry need In-House Testing?

II. The BSS 2000 Cannabis Analyzer

A. How it Works

B. Establishing Spectrometer Street Cred

C. Cannabinoids in Oils Results

D. Terpenes in Oils Results

E. Cannabinoids in Cannabis Buds and Solids Results

III. Applications

IV. Conclusions

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The State of Cannabis Testing➢ There are too frequent press reports of mis-labeling

problems in the cannabis industry, due in part to

– Unrepresentative Sampling

– Cannabis instability

– Lack of standardized testing

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Food Industry Testing

Grain Elevator

Mill

Dairy

Moo!

= IR spectrometer

testing

= Where infrared analyzers determine

price, quality, and process control

5

Cannabis Industry Testing

DispensaryRaw Material

Extractor Edibles Mfg.

Where are the infrared

analyzers to determine

potency, quality, and process

control?

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The BSS 2000 Cannabis Analyzer

Your Complete Cannabis Analysis System

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How it Works I

1. Patent Pending – United States non-provisional utility patent applied

for

2. A general purpose quantitative mid-infrared spectrometer.

3. Mid-infrared is 10x-100x more sensitive and more selective than near

infrared

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How it Works IICtrl+Click on the link below to view a video at our YouTube

channel on how to use the BSS 2000 Cannabis Analyzer.

BSS 2000 Cannabis Analyzer How to Use It Video

Big Sur Scientific YouTube Channel

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Experimental▪ Mid-infrared spectra measured in triplicate using the BSS 2000

Cannabis Analyzer from Big Sur Scientific

1250-952 cm-1, where cannabinoids strongly absorb

12 cm-1 instrumental resolution

MIR More sensitive and selective than NIR

▪ Analyzed 7 cannabis oil samples from Pop NaturalsCO2 oil not winterized but undergoes a “proprietary process”

▪ Cannabinoid and Terpene profiles measured by chromatography CW

Analytical, Oakland CA

▪ Chemometric models built using infrared spectra and chromatographic

data in Big Sur Scientific Model Builder SoftwarePLS-1 algorithm

Individual models built for each algorithm

Generally 4-5 principal components used

Savitsky-Golay 1st derivative used in some cases

▪ 2 minutes/sample, push button operation

▪ Only need a few mgs of sample

▪ Calibrations customized to customers products and trusted lab

▪ All stated accuracies are averaged cross validation standard

error of predictions

10Wavenumbers [1/cm]

1240 1220 1200 1180 1160 1140 1120 1100 1080 1060 1040 1020 1000 980 960

arb

itr. u

nits

0.44

0.42

0.4

0.38

0.36

0.34

0.32

0.3

0.28

0.26

0.24

0.22

0.2

0.18

0.16

0.14

0.12

0.1

Spectrometer Street Cred - The Purell Test

Known volume % Ethanol21 Data Points

BS

S 2

000 R

esu

lts

Test Quantitative abilities of the

BSS 2000

70% Ethanol label claim

Diluted Purell sample volumetrically

7 standards measured in triplicate

R2 = 0.999

Accuracy = ±1.2%

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Spectra Stable Over Time

22 Spectra of same sample collected over 3 months

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Oils Analysis - Cannabinoids

HPLC vs. BSS 2000 Results THCA in Oils

21 Data Points

BS

S 2

000 R

esu

lts

HPLC Results

Cannabinoid Range in Wt. % R2 Accuracy* THCA 0.4 – 38.1 0.99 ±1.3

THC 2.8 – 25.6 0.99 ±1.2

CBDA 0.0 – 35.0 0.99 ±0.5

CBD 0.1 – 40.9 0.99 ±0.2

CBGA 0.0 – 1.8 0.99 ±0.1

CBG 0.4 – 1.0 0.93 ±0.09

CBN 0.1 – 0.8 0.99 ±0.03

CBC 0.6 - -1.9 0.99 ±0.06

THCV 0.0 – 0.2 0.99 ±0.01

*Average Cross Validation Standard Error of Prediction

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Oils Analysis - Terpenes

Analyte R2 Accuracy, Wt. %

Myrcene 0.99 ±0.05

Beta-Carophyllene 0.99 ±0.05

Limonene 0.99 ±0.05

Alpha-Pinene 0.93 ±0.06

Linalool 0.98 ±0.05

Alpha-Humulene 0.99 ±0.05

Nerolidol 0.99 ±0.05

Alpha-Terpinene 0.99 ±0.05

Gamma-Terpinene 0.99 ±0.05

Terpinolene 0.99 ±0.05

Camphene 0.99 ±0.05

HPLC vs. BSS 2000 Results β-Caryophyllene in Oils

21 Data Points

GC Results

BS

S 2

000 R

esu

lts

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Cannabinoids in Bud

HPLC vs. IR Results,

THCA in Buds

29 Data Points

HPLC Results

BS

S 2

000 R

esu

lts

Analyte R2 Accuracy, Wt. %

THCA 0.98 ±1.5

THC 0.98 ±0.26

CBDA 0.98 ±0.9

CBD 0.98 ±0.1

CBN 0.98 ±0.05

HPLC vs. IR Results,

THCA in Buds

29 Data Points

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Cannabinoids in Cannabis Solids

HPLC vs. IR Results

THCA in Shatter/Resin/Sugar/Wax

27 Data Points

Analyte R2 Accuracy, Wt. %

THCA 0.99 ±1.9

THC 0.91 ±0.4

CBDA 0.99 ±0.62

CBD 0.94 ±0.1

CBN 0.8 ±0.1

HPLC Results

BS

S 2

000 R

esu

lts

Cannabis Solids = Shatter, resin, rosin,

wax, & sugar

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BSS 2000 Applications

DispensaryRaw Material

Extractor Edibles Mfg.

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Conclusions

1. There is a need in the Cannabis industry for more in-

house potency testing at more times and in more places

than is currently available

2. The BSS 2000 accurately determines 9 cannabinoids and

11 terpenes in cannabis oils giving a cannabinoid and

terpene profile

3. The BSS 2000 accurately determines THCA, THC, CBDA,

CBD, and CBN in buds

4. The BSS 2000 accurately determines THCA, THC, CBDA,

CBD, and CBN, and in shatter/resins/wax/sugar/rosin

5. Allows truly representative sampling

6. Allows cannabis businessed to perform potency testing in

times and places previously thought impossible

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Thank You!

Big Sur Scientific

brian@bigsurscientific.com

(831) 205-8203