measurement of cannabinoid and terpene profiles in
TRANSCRIPT
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Measurement of Cannabinoid and Terpene
Profiles in Cannabis Extracts by Mid-Infrared
Spectroscopy
Brian C. Smith, Ph.D.
Chief Technical Officer
Big Sur Scientific
(508) 579-6514
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Outline
I. Does the Cannabis Industry need In-House Testing?
II. The BSS 2000 Cannabis Analyzer
A. How it Works
B. Establishing Spectrometer Street Cred
C. Cannabinoids in Oils Results
D. Terpenes in Oils Results
E. Cannabinoids in Cannabis Buds and Solids Results
III. Applications
IV. Conclusions
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The State of Cannabis Testing➢ There are too frequent press reports of mis-labeling
problems in the cannabis industry, due in part to
– Unrepresentative Sampling
– Cannabis instability
– Lack of standardized testing
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Food Industry Testing
Grain Elevator
Mill
Dairy
Moo!
= IR spectrometer
testing
= Where infrared analyzers determine
price, quality, and process control
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Cannabis Industry Testing
DispensaryRaw Material
Extractor Edibles Mfg.
Where are the infrared
analyzers to determine
potency, quality, and process
control?
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The BSS 2000 Cannabis Analyzer
Your Complete Cannabis Analysis System
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How it Works I
1. Patent Pending – United States non-provisional utility patent applied
for
2. A general purpose quantitative mid-infrared spectrometer.
3. Mid-infrared is 10x-100x more sensitive and more selective than near
infrared
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How it Works IICtrl+Click on the link below to view a video at our YouTube
channel on how to use the BSS 2000 Cannabis Analyzer.
BSS 2000 Cannabis Analyzer How to Use It Video
Big Sur Scientific YouTube Channel
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Experimental▪ Mid-infrared spectra measured in triplicate using the BSS 2000
Cannabis Analyzer from Big Sur Scientific
1250-952 cm-1, where cannabinoids strongly absorb
12 cm-1 instrumental resolution
MIR More sensitive and selective than NIR
▪ Analyzed 7 cannabis oil samples from Pop NaturalsCO2 oil not winterized but undergoes a “proprietary process”
▪ Cannabinoid and Terpene profiles measured by chromatography CW
Analytical, Oakland CA
▪ Chemometric models built using infrared spectra and chromatographic
data in Big Sur Scientific Model Builder SoftwarePLS-1 algorithm
Individual models built for each algorithm
Generally 4-5 principal components used
Savitsky-Golay 1st derivative used in some cases
▪ 2 minutes/sample, push button operation
▪ Only need a few mgs of sample
▪ Calibrations customized to customers products and trusted lab
▪ All stated accuracies are averaged cross validation standard
error of predictions
10Wavenumbers [1/cm]
1240 1220 1200 1180 1160 1140 1120 1100 1080 1060 1040 1020 1000 980 960
arb
itr. u
nits
0.44
0.42
0.4
0.38
0.36
0.34
0.32
0.3
0.28
0.26
0.24
0.22
0.2
0.18
0.16
0.14
0.12
0.1
Spectrometer Street Cred - The Purell Test
Known volume % Ethanol21 Data Points
BS
S 2
000 R
esu
lts
Test Quantitative abilities of the
BSS 2000
70% Ethanol label claim
Diluted Purell sample volumetrically
7 standards measured in triplicate
R2 = 0.999
Accuracy = ±1.2%
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Spectra Stable Over Time
22 Spectra of same sample collected over 3 months
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Oils Analysis - Cannabinoids
HPLC vs. BSS 2000 Results THCA in Oils
21 Data Points
BS
S 2
000 R
esu
lts
HPLC Results
Cannabinoid Range in Wt. % R2 Accuracy* THCA 0.4 – 38.1 0.99 ±1.3
THC 2.8 – 25.6 0.99 ±1.2
CBDA 0.0 – 35.0 0.99 ±0.5
CBD 0.1 – 40.9 0.99 ±0.2
CBGA 0.0 – 1.8 0.99 ±0.1
CBG 0.4 – 1.0 0.93 ±0.09
CBN 0.1 – 0.8 0.99 ±0.03
CBC 0.6 - -1.9 0.99 ±0.06
THCV 0.0 – 0.2 0.99 ±0.01
*Average Cross Validation Standard Error of Prediction
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Oils Analysis - Terpenes
Analyte R2 Accuracy, Wt. %
Myrcene 0.99 ±0.05
Beta-Carophyllene 0.99 ±0.05
Limonene 0.99 ±0.05
Alpha-Pinene 0.93 ±0.06
Linalool 0.98 ±0.05
Alpha-Humulene 0.99 ±0.05
Nerolidol 0.99 ±0.05
Alpha-Terpinene 0.99 ±0.05
Gamma-Terpinene 0.99 ±0.05
Terpinolene 0.99 ±0.05
Camphene 0.99 ±0.05
HPLC vs. BSS 2000 Results β-Caryophyllene in Oils
21 Data Points
GC Results
BS
S 2
000 R
esu
lts
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Cannabinoids in Bud
HPLC vs. IR Results,
THCA in Buds
29 Data Points
HPLC Results
BS
S 2
000 R
esu
lts
Analyte R2 Accuracy, Wt. %
THCA 0.98 ±1.5
THC 0.98 ±0.26
CBDA 0.98 ±0.9
CBD 0.98 ±0.1
CBN 0.98 ±0.05
HPLC vs. IR Results,
THCA in Buds
29 Data Points
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Cannabinoids in Cannabis Solids
HPLC vs. IR Results
THCA in Shatter/Resin/Sugar/Wax
27 Data Points
Analyte R2 Accuracy, Wt. %
THCA 0.99 ±1.9
THC 0.91 ±0.4
CBDA 0.99 ±0.62
CBD 0.94 ±0.1
CBN 0.8 ±0.1
HPLC Results
BS
S 2
000 R
esu
lts
Cannabis Solids = Shatter, resin, rosin,
wax, & sugar
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BSS 2000 Applications
DispensaryRaw Material
Extractor Edibles Mfg.
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Conclusions
1. There is a need in the Cannabis industry for more in-
house potency testing at more times and in more places
than is currently available
2. The BSS 2000 accurately determines 9 cannabinoids and
11 terpenes in cannabis oils giving a cannabinoid and
terpene profile
3. The BSS 2000 accurately determines THCA, THC, CBDA,
CBD, and CBN in buds
4. The BSS 2000 accurately determines THCA, THC, CBDA,
CBD, and CBN, and in shatter/resins/wax/sugar/rosin
5. Allows truly representative sampling
6. Allows cannabis businessed to perform potency testing in
times and places previously thought impossible
