mbv2010/bio2140 colloquium, february 19. rules multiple choice only one correct answer 30-60 seconds...

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MBV2010/BIO2140

Colloquium, February 19

RULES

• Multiple choice• Only one correct answer• 30-60 seconds to answer• No textbook, computer, mobile phone, please

There will be a prize for the group with the most correct answers.

Example

Below is a series of equations. Which numberswould fit logically into the last equation ?(13 – 4) : 9 = 1 (27 – 9) : 9 = 2(87 – 15) : 9 = 8 (68 - ??) : 9 = ??

A (68 - 14) : 9 = 6

B (68 - 5) : 9 = 7

C (68 - 23) : 9 = 5

D (68 - 32) : 9 = 4

0

Example

Below is a series of equations. Which numberswould fit logically into the last equation ?(13 – 4) : 9 = 1 (27 – 9) : 9 = 2(87 – 15) : 9 = 8 (68 - ??) : 9 = ??

A (68 - 14) : 9 = 6

B (68 - 5) : 9 = 7

C (68 - 23) : 9 = 5

D (68 - 32) : 9 = 4

0

Dideoxynucleotides are used in

A DNA sequencing

B Cloning of DNA

C PCR

D Southern hybridization

1

Dideoxynucleotides are used in

A DNA sequencing

B Cloning of DNA

C PCR

D Southern hybridization

1

Ribosomal RNAs

A code for ribosomal proteins

B function in transcription of genes

C are absent from mitochondria

D function in protein synthesis

2

Ribosomal RNAs

A code for ribosomal proteins

B function in transcription of genes

C are absent from mitochondria

D function in protein synthesis

2

The smallest human chromosome has a size of

A 32 x 106 bp

B 41 x 106 bp

C 47 x 106 bp

D 58 x 106 bp

3

The smallest human chromosome has a size of

A 32 x 106 bp

B 41 x 106 bp

C 47 x 106 bp

D 58 x 106 bp

3

The polymerase chain reaction (PCR) is used for

A amplifying proteins

B copying plasmids

C copying RNA

D amplifying DNA

4

The polymerase chain reaction (PCR) is used for

A amplifying proteins

B copying plasmids

C copying RNA

D amplifying DNA

4

DNA ligases

A catalyze the formation of ß-N- glycosidic bonds

B play a role in transcription of DNA

C catalyze the formation of phosphodiester bonds

D catalyze the formation of hydrogen bonds

5

DNA ligases

A catalyze the formation of ß-N- glycosidic bonds

B play a role in transcription of DNA

C catalyze the formation of phosphodiester bonds

D catalyze the formation of hydrogen bonds

5

Van der Waal’s forces

A are attractive forces between atoms

B are 10 times weaker than covalent bonds

C are strongest at an atom distance of ≈ 0.2 nm

D play a role in stabilization of the DNA double helix

6

Van der Waal’s forces

A are attractive forces between atoms

B are 10 times weaker than covalent bonds

C are strongest at an atom distance of ≈ 0.2 nm

D play a role in stabilization of the DNA double helix

6

What is shown in this figure?

A a random RNA structure

B a microRNA structure

C a small nuclear RNA structure

D a tRNA structure

7

What is shown in this figure?

A a random RNA structure

B a microRNA structure

C a small nuclear RNA structure

D a tRNA structure

7

Protein structures can be determined by

A mass spectrometry

B 2D-polyacrylamide gel electrophoresis

C x-ray diffraction

D liquid chromatography

8

Protein structures can be determined by

A mass spectrometry

B 2D-polyacrylamide gel electrophoresis

C x-ray diffraction

D liquid chromatography

8

The amino acid sequence of proteins can bedetermined by

A mass spectrometry

B 2D-polyacrylamide gel electrophoresis

C x-ray diffraction

D liquid chromatography

9

The amino acid sequence of proteins can bedetermined by

A mass spectrometry

B 2D-polyacrylamide gel electrophoresis

C x-ray diffraction

D liquid chromatography

9

Centromeres

A are present in all chromosomes

B are normally in the center of chromosomes

C are nucleosome-free sequences

D normally contain repetitive DNA

10

Centromeres

A are present in all chromosomes

B are normally in the center of chromosomes

C are nucleosome-free sequences

D normally contain repetitive DNA

10

SWI/SNF is a

A histone modification complex

B nucleosome remodeling complex

C nucleosome assembly complex

D none of the above

11

SWI/SNF is a

A histone modification complex

B nucleosome remodeling complex

C nucleosome assembly complex

D none of the above

11

What is shown in this figure?

A histone modifications in telomeres

B histone modifications in protein-coding genes

C replacement of histone H3

D replacement of histone H2A

12

What is shown in this figure?12

A histone modifications in telomeres

B histone modifications in protein-coding genes

C replacement of histone H3

D replacement of histone H2A

The average size of genes in bacterial genomes is

13

A 1000 – 1500 bp

B 500 – 1000 bp

C 3000 – 4000 bp

D 1500 – 3000 bp

The average size of genes in bacterial genomes is

13

A 1000 – 1500 bp

B 500 – 1000 bp

C 3000 – 4000 bp

D 1500 – 3000 bp

Table 8.2 part 1 of 2 Genomes 3 (© Garland Science 2007)

Vibrio cholerae

Deinococcusradiodurans

(Conan the bacterium)

What is the role of the SeqA protein in E. coli ?

14

A initiate replication

B prevent replication

C break hydrogen bonds

D relieve topological stress in DNA

What is the role of the SeqA protein in E. coli ?

14

A initiate replication

B prevent replication

C break hydrogen bonds

D relieve topological stress in DNA

SeqA

SeqA binds to hemimethylated DNA

CH3

When in a cell cycle is the eukaryotic origin recognition complex bound to DNA?

15

A in G1, S, and G2

B in S, G2, and M

C in G2, M, and G1

D only at the end of G1

When in a cell cycle is the eukaryotic origin recognition complex bound to DNA?

15

A in G1, S, and G2

B in S, G2, and M

C in G2, M, and G1

D only at the end of G1

Which of the following proteins is involvedin initiation of replication in the nucleus?

16

A DnaA

B Type II topoisomerases

C Cdc6

D Cdc45

Which of the following proteins is involvedin initiation of replication in the nucleus?

16

A DnaA

B Type II topoisomerases

C Cdc6

D Cdc45

Replication protein A (RPA) is a17

A helicase

B topoisomerase

C single-strand binding protein

D DNA polymerase

Replication protein A (RPA) is a17

A helicase

B topoisomerase

C single-strand binding protein

D DNA polymerase

The proliferating cell nuclear antigen (PCNA)is involved in

18

A cell division

B nucleosome assembly

C genome replication

D formation of

heterochromatin

The proliferating cell nuclear antigen (PCNA)is involved in

18

A cell division

B nucleosome assembly

C genome replication

D formation of

heterochromatin

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