manipulating molecules
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Manipulating molecules
Biot-111Lecture 4
Types of molecules• Nucleic Acids
– Deoxyribonucleic acid (DNA)• Chromosomal DNA• Mitochondrial DNA (mtDNA, or less popularly, mDNA) • Chloroplast DNA
– Ribonucleic acid (RNA)• Ribosomal RNA (rRNA)• Messenger RNA (mRNA) • Transfer RNA (tRNA)
– Complementary DNA (cDNA) RNA-DNA Hybrid
• Proteins– Structural proteins
• E.g. Membrane proteins
– Functional proteins • E.g. Enzymes
» Helicases
» Transferases
» Proteases
» Nucleases
» Kinases
» Phosphatases etc.
Manipulating Nucleic Acids / cloning
Isolation and Purification of Nucleic Acid
Restriction analysis
Selection of suitable vector
Recombination
Insertion into a suitable host
Screening and selection of recombinants
The tools of recombinant DNA technology
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