manipulating molecules

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Manipulating molecules Biot-111 Lecture 4

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Manipulating molecules. Biot-111 Lecture 4. Types of molecules. Nucleic Acids Deoxyribonucleic acid (DNA) Chromosomal DNA Mitochondrial DNA (mtDNA, or less popularly, mDNA) Chloroplast DNA Ribonucleic acid (RNA) Ribosomal RNA (rRNA) Messenger RNA (mRNA) Transfer RNA (tRNA) - PowerPoint PPT Presentation

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Page 1: Manipulating molecules

Manipulating molecules

Biot-111Lecture 4

Page 2: Manipulating molecules

Types of molecules• Nucleic Acids

– Deoxyribonucleic acid (DNA)• Chromosomal DNA• Mitochondrial DNA (mtDNA, or less popularly, mDNA) • Chloroplast DNA

– Ribonucleic acid (RNA)• Ribosomal RNA (rRNA)• Messenger RNA (mRNA) • Transfer RNA (tRNA)

– Complementary DNA (cDNA) RNA-DNA Hybrid

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• Proteins– Structural proteins

• E.g. Membrane proteins

– Functional proteins • E.g. Enzymes

» Helicases

» Transferases

» Proteases

» Nucleases

» Kinases

» Phosphatases etc.

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Manipulating Nucleic Acids / cloning

Isolation and Purification of Nucleic Acid

Restriction analysis

Selection of suitable vector

Recombination

Insertion into a suitable host

Screening and selection of recombinants

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The tools of recombinant DNA technology

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