macromolecular nanomachines in action

IN ACTION

José Ramón Macías

macromolecularnanomachines

José Ramón Macías

Hi, my name is:

Bioinformatician

macromolecularnanomachines

IN ACTION

Structure

Sequence

Structure

Function

Sequence

10 nm

1 mm

100 nm

X-Ray DiffractionCrystallography

conformational changes

Simian Virus 40

Large T antigen

Simian virus 40(SV40)

Large T antigen

Short & straight

Long & straight

Middle curvature

High curvature

Short & straight

Long & straight

Middle curvature

High curvature

Bacteriophage SPP1 replicative

Helicase G40P

D. melanogaster

26S proteasome

26S proteasome

X-Ray Crystallography

Requires Crystals

High Resolution

X-Ray Crystallography

Requires Crystals

High Resolution

3D Electron Microscopy

Medium Resolution

Crystals Not required

X-Ray Crystallography

Requires Crystals

High Resolution

3D Electron Microscopy

Medium Resolution

Crystals Not required

X-Ray Crystallography

Requires Crystals

High Resolution

3D Electron Microscopy

Medium Resolution

Crystals Not required

X-Ray Crystallography

Requires Crystals

High Resolution

3D Electron Microscopy

Medium Resolution

Crystals Not required

Hybrid Models

X-Ray Crystallography

Requires Crystals

High Resolution

3D Electron Microscopy

Medium Resolution

Crystals Not required

Hybrid Models

Fitting

3D-EM model Atomic model

Hybrid model

GroEL chaperonin

Anti-tumoralP53 transcription factor

lectron

icroscopy

ata

ank

E

M

D

B

10

40

67

117

193

266

363

2002 2003 2004 2005 2006 2007 2008

since June 2002, the number of structures has increasedsteadily.

“

”

Single Particle Icosahedral

HelicalIndividual Structure 2D Crystal

Today there are629 entries

1 out of 3 structures

are covered by more than one entry

369

http://biocomp.cnb.csic.es/das/PeppeR

DASx3DEM & PeppeR

Structure

Sequence

adopting different conformations

is what leads to function