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Improving Efficiencies in the

Canada Wide Standard for Hydrocarbon Contamination in Soil Tier 1 method

using

advances in CDS data handling and reporting

Darren JohnstonApplications ChemistVarian Canada

The 42nd AnnualThe 42nd Annual

April 23April 23--26, 200726, 2007

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Overview of Application: C10-C50 in soil

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Challenges with CCME

•Integration: Achieve reproducible area counts without subjective manual integration

•Identification: CCME Fractions are based on apex RT of hydrocarbons

•Calibration: Average RF of C10, C16, C34 with min. 3 point linearity

•Quantitation: Average RF used against the total area for each identified fraction

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Goals Using Galaxie

•Integration: Method defined time slice with horizontal baseline

•Identification: peak and group table to define CCME regions

•Calibration: Average RF is an available feature in Galaxie

•Quantitation: CCME fraction results are calculated in Galaxie

improve efficiencyof CCME analysis

Galaxie.lnk

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Galaxie – Quick Overview of Key Features

Menus

Icon bars

Chromatogram Window

Browser

Status/Processing Parameters Window

Status Bar

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Integration and Identification

•Fraction #2: Area counts from the apex of nC10 to the apex of the nC16

•Fraction #3: Area counts from the apex of nC16 to the apex of the nC34

•Fraction #4: Area counts from the apex of nC34 to the apex of the nC50

•Time slice integration feature defines RT regions in the chromatogram

•Use peak table to name the individual time slice regions

•Use group table to define CCME fractions #2-#4

•Calibration check boxes appear on peak and group tables

Galaxie FeaturesGalaxie Features

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Integration challenges with HC samples

•Inconsistent baseline treatment can force the use of corrective manual integration

•Time consuming

•Decreased reproducibility – subjective to the user

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Galaxie Solution – Time Slice with Horizontal Baseline

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Peak ID using time slice on standards

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Peak Table in Galaxie

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Group Table in Galaxie

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Another Group Table in Galaxie

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CCME Calibration for F2-F4

•Standards are equal amounts of nC10, nC16 and nC34. (min 3 point linearity)

•The average RF for nC10,nC16 and nC34 (%RSD<10%) is used for fraction quantitation

•nC50 RF must be a within 30% of the average of nC10, nC16 and nC34 RF

•Performance spikes must be recovered within 20%

Galaxie FeaturesGalaxie Features

•Calibration check box on peak and group tables

•Reference response factors used in calibration table

•Average RF available as calibration model, %RSD automatically calculated

•Variable editor used to create custom calculations

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Calibration Table in Galaxie

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Calibration Table in Galaxie cont’d

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Galaxie Variable Editor

•set up custom calculations to test for the Prescriptive Elements of CCME

•Example RF of C50 within 30% of average RF of C10, C16 and C34

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Galaxie Results Page

•Customize the variables displayed

•Allows the user to read the results right off the page

•No EXCEL calculations required

•Print, PDF, or export to LIMS

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Galaxie System Suitability

•Allows the user to set up automated PASS/FAILParameters for some Prescriptive Elements of CCME

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High Temperature Hardware

Varian 3800

•Cold on Column

•Large Volume Injection

•Electronic Flow Controller

•Optimized Injection Liner

•CP-Select Mineral Oil Column

•Oven Compartment Reducer

•High Temperature FID

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CCME Standard using LVI

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Acknowledgements

Mark DalmaijerManitoba Hydro Lab ServicesWinnipeg, MB

Charles van Dortmond Environment Market Development ManagerVarian Inc.Middelburg, The Netherlands

Brooke BennettKomex / University of AlbertaDept of Environmental Eng.Edmonton, AB

Adam GillespieUniversity of SaskatchewanDept of Soil SciencesSaskatoon, SK

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Thank you for your attention

Western Canadian Sales Team

Blair AlbrechtMark CullenJason DunbarSteve HoseinDarren Johnston

Western Canadian Service Team

Darryl BilanWayne GroutRob ChristianBrian GoertzenCristian VinturacheDerek SalomonsWayne Blonski