higher content yeast imaging and data processing richard alexander stowers microscopy center may 19,...

Higher Content Yeast Imaging and Data Processing

Richard Alexander

Stowers Microscopy Center

May 19, 2009

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

Control Plate Experimental Plate

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

High Content Imaging Candidates

• Reproducible

• Segmentable

• Automatable– Acquisition– Analysis

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

Use VBA Code to…

• Group Colonies

• Classify Colonies

• Compare between control and experimental plates

A B C123

Summary

• Yeast Robot– Pin yeast plate

• Flatbed Scanner– Image yeast plate

• Axiovision– Automate Segmentation– Automate Data Acquisition

• Excel– Automate Data Processing

Acknowledgements

• Rong Li– Das Arupratan– Brian Slaughter

• Molecular Biology– Dan Bradford– Brian Sanderson