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Fantastic Properties of Nano-bubbles

Kaneo Chiba Masayoshi Takahashi

Shrinking   

Disappear 

Microbubble     Nano‐bubble    

Continue 

Ordinary bubble (Macrobubble)

Goes up rapidly

Burst at the surface

３

Microbubble

Collapse

Physical stimulus

Nano-bubble production by collapsing microbubble

Radical generation

・OH

Decomposition of organic chemicals

Waste water treatment

Physical stimulus

Nanobubble production

Collapse

Microbubble

with salt (electrolytes)

Nano-bubble

・OH

４

Nono-bubble generator

MicrobubbleNano-bubble

Physical stimulus

５

Analysis of nano-bubble by Dynamic light scattering (DLS)

0

2

4

6

8

10

12

14

16

18

20

76 93 113 138 168 204 249 304 370

Particle size (nm)

６

Microbubble 10 ～30μm

Nano-bubble< 200nm

The deference of size between MB and NB

７

Shrinking

Disappear

Several minutes Several months

Microbubble Nanobubble

Continue

The deference of life time between MB and NB

Why ? ８

Movement of microbubbles in electrophoresis cell

Light

Microscope

Electrode

Electrophoresis cell Water + microbubble

Electrode ( + ↔ − ) (− ↔ +)

９

time

ζpo

tent

ial（

mV

）

Change in ζ potential of microbubble over time

Diameter （μm）

0 5 10 15 20 25 30-20

-30

-40

-50

-60

-70

-80

Accumulation of surrounding ions

Ions Microbubble

time Ions

１０

with salt (electrolytes)

Aqueous solution

Nano-bubble

＋

＋ ＋

＋

＋

＋

＋－－

－－

－－－

－

＋

＋

＋

＋

＋

－

＋

－

＋

＋

＋

＋

＋

＋ ＋

Stabilization mechanism of nanobubble (assumption)

Negatively charged

Surrounded by accumulated ions(counter ions)

Prevention of gas dispersion

Salting out

１１

Nano-bubble generator

１２

Normal ozone water vs. Ozone nano-bubble

Ozone water ; disinfection & sterilizing （Food industry, medical ）

1 hour

100 days

Bubbling

Micro-bubble Nano-bubble

0.1mg/L

1.5mg/L

1.5mg/L

More than 1.0mg/L

Ozone

Normal method

Nano-bubble

Collapse

13

Oyster

Norovirus

Norovirus symptoms:vomiting, diarrhea, a low-grade fever, chills, headache, muscle aches

During cultivation

How to treat the problem of norovirusHow to treat the problem of norovirus

14

Chlorine ( high concentration )----- not good for foods

Chlorine

Norovirus

Conventional method

Oyster

Ozone water Oyster Ozone = strong oxidation ability

↓Virus → inactivation Norovirus 15

Oysters in ozone nanobuble water for 1 day

16

Before ozone nanobubble treatment

17

More than 99% of feline calicivirus in the body --- inactivated

After ozone nanobubble treatment

18

Practical method for inactivation of norovirus by ozone nano-bubble

19

Carps : recovering from Carp herpes ( serious disease )

Active effect for fishes

20

Oxidation ability of ozone nano-bubble as a form of mist 21

more than 80% of original oxidation ability

reduction of smell and bacteria from atmospheres 22

Oxygen nanobubble

Collapse of oxygen microbubble About 1% salt Recovery of weakened fishes

Active effect for creatures

23

Nano-Bubble Aquarium 24

Orchid in oxygen NB water for more than 1 month

Astonishing effects on plant

Still alive

25

Tap water Oxygen nano-bubble water

White radish Broccoli Broccoli

White radish

Initial growth of plants

26

Active effect for oysters

-20℃ for 1 day in oxygen NB

Room temperature

After 1 hour

Still alive

27

control TNFalpha

With oxygen nanobubble

control TNF alpha

Prevention of arteriosclerosis (hardening of blood vessel )

Oxygen nanobubble inhibits mRNA expression induced by TNFα in RAOECs

ICAM1

VCAM1

GAPDH

Department of Cardiology,Jichi Medical University

Application of nanobubble in medicine

Culture aorta endothelium of rat Cytokine stimulation（TNF alpha）

Heart attack

Stroke

28

Vagus nerve of rat : after 1 week

Isotonic NaCl solution Oxygen nanobubble

Tokyo Medical and Dental University

Application of nanobubble in medicine

Preservation of living tissue / organ

29

30

Thank you very much