environmental control for a chip scale laboratory team: robert bouda - leader ashok rajan –...

ENVIRONMENTAL CONTROL FOR A CHIP SCALE

LABORATORY

Team:

Robert Bouda - Leader

Ashok Rajan – Communication Liaison

Sean Belieu – Webmaster

Sahan Fernando

Tianyu Wang

Zhaokui Wang

Faculty Advisor & Client: Dr. Liang Dong

System DescriptionProblem Need Statement:

A greenhouse is costly, space intensive, and we are looking to solve it.

Functional Requirements

• Solenoid Control System1. A system using Verilog or VHDL capable

of receiving and decoding a serial digital signal

• Sensor Array1. A control element that sends data in a serial

digital format to a different system designed to regulate environmental conditions

• Channel and Chamber fabrication1. A flow channels capable of delivering liquid

to individual chambers at volumes of 1µm3.

2. 7 air flow channels designed to depress and restrict flow through liquid channels using air pressure.

Risks

• Channel thickness

Solenoid Valves

Solenoid ValvesThe connections with the chip are done using thin tubing and syringes:

Solenoid Valves

Clippard(12-ET-2M-6)• Extremely basic solenoids• 25-pin interface• Multisim – Verilog

Existing technology:• Fluidigm

Genetic Analysis

Work plan – Subgroup 1• This semester:

Design the control logic of solenoid valves to:

a) Control the liquid flow into the growth chambers

b) Select a specific growth chamber out of 128 growth chambers that are fabricated on an interface.

• Next semester:• Intergrate sensor unit and fabricated channels with Solenoid

control

Sensor Unit

The sensor detects temperature/ humidity and outputs the data via serial DATA to a micro-controller.

This data will be outputted via the micro-controller in binary form to the solenoid control system.

Work plan – Subgroup 2This semester:

• A sensor unit that will successfully read environmental Temperature/Humidity and output data to the control system

Current:• Actual circuit design• Coding the Micro-controller

Next Semester:• Intergrate sensor unit with Solenoid control

• There are 128 micro-greenhouses totally .• And there are 128 channel connect to each of them.• 7 bits are used to make them work individually.(2^7=128)• (As shown above)

Microfluidics Channels

Fabrication process

Firstly, we fabricate our molds on glass slide by using IBA.

Then Once the slides are ready, we used as molds for PDMS, when the control and flow layers is prepared separately we bond them together crossing to each other.

Two layers device

• The designed chip has two layers:

• flow level(Top), where the cells will ultimately grow.

• control layer(Under), used to close and open certain valves by putting under pressure the thin membrane between the control and the flow layers.

Work plan – Subgroup 3This semester:

• Learning how to fabricate.• Learn fabrication with the IBA(isobornyl acrylate) process• Learn PDMS(Polydimethylsiloxane) fabrication process• Make a two layers devices and perform tests on it to ensure

quality of devices.

Next Semester:• Ensure interface that was created serves as a good mediator

between the other two sub projects.

Projected CostsMaterial Product (Not Final) Cost ( Estimation)

Solenoid Valves (Qty-12) Clippard12 ET – 2M – 6

$340.00

Electronic Manifold (Qty-1) ClippardEMC – 12 – 06 – 20

$260.00

Temeprature & Humidity sensor (Qty-1)

SensironSHT11

$40.00

Tubing Adaptors (Qty-12) -   $30.00

Tubing Cole PalmerEW-06418-02

Donated by Client

IBA Fabrication Glass - Donated by Client

MCU for Sensor and Solenoids - Currently Available Lab Equipment

  Total $670

Final DeliverablesThe expected end product will consist of three sub-systems that

will be combined to form the complete chip-scale greenhouse system

End Product• Temperature and Humidity Sensing Unit • Solenoid Control System• Liquid flow channels

Questions?