compact - wydział farmaceutycznylc-ms/ms. now, the benefits of full-scan data and precise mass...
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LC-MS/MSInnovation with Integrity
Instant expertise in accurate mass LC-MS/MS
compact
Bru
ker
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Research-Grade Technology Meets High Productivity
Instant expertise
You expect the fullest possible insight into your sample. compact delivers the highest quality of results without compromise. All specified performance parameters simultaneously delivered in a single run with no need for tuning or optimization is what makes compact unique.
Bruker‘s „one-shot analysis“ philosophy is embodied in compact, accelerating productivity by enabling every analyst to deliver expert calibre results even on the first run.
compact is teamed with world leading application solution software. Your expert partner, whatever your challenge. Synthetic chemistry confirmation, advanced screening and identification, proteomics and metabolomics, or intact protein and biopharmaceutical analysis compact delivers instant expertise to your laboratory.
The Ultra-High Resolution (UHR) TOF technology pioneered by Bruker sets new standards and expectations as to what can be achieved using accurate mass LC-MS/MS.
The power of technology demanded for research applications is now available in a com-pact, robust and economic bench-top system. Research-grade technology applied to every routine analytical task. The new Bruker compact QqTOF features:
• 50 GBit/sec sampling technology maintain-ing full specification resolution even during ultrafast chromatography
• Increased dynamic range for definitive trace analysis from complex, high-background matrices
• Low picogram sensitivity to rival the best triple-quadrupole sensitivity while delivering full scan, precise mass data
• Superior 1 ppm mass accuracy ensured by automated calibration
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Engineered for Dependable Precision
Optimize your LCMS methods without compromising: compact™ delivers all specified performance parameters simultaneously to solve your analytical challenges.
Full Sensitivity Resolution – Having to choose between resolution and sen-sitivity on other instruments restricts depth to which you can understand your sample.
Enhanced dynamic range during real LC time scales – Greatly increased robustness to sample variation allowing reduced sample pretreatment especially desirable in high throughput quantitative applications.
One shot plug & play acquisition with triple-quad standard sensitivity –Ensuring qualitative and quantitative results in one LC run with fastest time-to-success.
Let the compact™ achieve your goals in:
Synthetic chemistry support Forensics and doping control Drug metabolite, degradant and
impurity identification and quantitation Biomarker discovery and validation in
proteomics and metabolomics Intact protein analysis and characteriza-
tion of biopharmaceuticals
Flight Tube with in-flight focussing for full sensitivity resolution
New 10bit ADC digitizer
High Transmission CID Cell
Quadrupole
Patented Dual Ion Funnel
ESI Source
Dual Stage Reflector
Don‘t accept compromise – Go for Instant Expertise!
There is more to successful analysis than hitting the mark on one performance parameter at a time.
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5 orders of magnitude intrascan dynamic range at LC speed
Advantages of new compact™ Time-of-Flight detection system
Very fast detection and digitizing techno-logy is key to maintaining full resolution at all acquisition speeds and provide high dynamic range to detect the smallest peaks in the presence of high-abundance background matrix. The new 10bit ADC digitizer also delivers Bruker’s renowned true isotopic pattern, an essential feature for de-novo chemical identification using Smartformula3D™.
Ultimate sensitivity
Groundbreaking sensitivity improvements bring compact’s trace analysis capabi-lities to levels usually associated only with targeted assays on triple quadrupole LC-MS/MS. Now, the benefits of full-scan data and precise mass confirmation can be brought to all routine screening assays.
The Key to Sensitivity and High Dynamic Range
Repetitive injection of reserpine displayed as BPC of m/z 609
11 12 13 14 15 Time [min]0.0
0.5
1.0
1.5
4x10Intens.
msSensitivity_DionexLC_QT3-pp03_HESI_000002.d: BPC 609.2807±0.008 +All MS
100 fg absolute reserpine withionbooster sourceS/N > 100:1 RMS
Intensity ratio = 7 155 285 / 46 = 1.6 x 105 @ 1Hz acquisition speed
195.0878
217.0696
233.0436
+MS, 4.2-4.3min #247-256
0
2
4
6
6x10Intens.
0 25 50 75 100 125 150 175 200 225 m/z
Caffeine 0.6ppm I = 7 155 285cts
90.0550
+MS, 4.2-4.3min #247-256
0
10
20
30
40
50
Intens.
86 88 90 92 94 96m/z
Alanine 0.5ppm I = 46ctsS/N > 8:1
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The Key to Sensitivity and High Dynamic Range
Your Partner in Chemistry Support
Your in-lab, publication-ready facility for synthetic chemical confirmation delivered and managed!
Compass OpenAccess™ provides an automated walk-up accurate-mass system for chemical formula identification, confirmation and general LC-MS measurements. The client-server based software supports a wide variety of workflows especially for synthetic and medicinal chemists with no MS expertise required. Immediate, publication quality accurate mass data reports are automatically emailed to the user, transmitted to e-labbook and archived. Compass OpenAccess has full group facility management tools vastly reducing the management burden of a multi-user core facility, whether in industry or university settings.
SmartFormula determination
Three dimensions of information simultaneously raise your analytical tasks to unrivaled heights of confidence:
Measure with superior accurate mass Validate with True Isotopic Pattern
(TIP) analysis Also benefit from accurate mass
and TIP in analysis of fragments in MS/MS mode
Mass accuracy, chemical knowledge and SmartFormula 3DTM clearly limit the number of possible formulae in molecular formula generation: for confident deter-mination of the elemental composition of a given peak.
Compass OpenAccess job results
# possible formulae
Mass Accuracyseveral ppm accuracy
plus Chemical Expertiseadditional confidence
plus SmartFormula (Mass Accuracy+TIP)
sub-ppm confidence
plus SmartFormula3D (fragments-based TIP)unambiguous formula ID
numerous
medium
few
single
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One Run Discovery and Validation of ...
5 orders of magnitude intrascan dynamic range at LC speedMulti-target screening on 10 ppb level
hrEIC of m/z 72.0444∆m/z = 0.5 ppm
High resolution Extracted Ion Chromatogram (hrEIC) identifying 60 pesticides in a single LC-TOF run. All analytes across the entire mass range of 70 m/z up to 900 m/z are detected with highest sensitivity maintaining full resolution at U-HPLC speed. The new CID cell with uncompromised mass transfer and sensitivity allows for small molecule screening, confirmation and quantitation in a single LC-TOF run.
Confirmation of molecular formula with instant accurate mass and isotopic fidelity
Measured (top) against theoretical spectrum (bottom) of Penconazole (C13H15Cl2N3) out of the same data set showing a perfect fit of isotopic ratios and spacing. SmartFormula™ does confirm the identification in spite of matrix background signals.
hrEIC of m/z 890.5267∆m/z = 1.7 ppm
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One Run Discovery and Validation of ... Biomarkers and Small Molecules
In-depth identification analysis of complex proteomics samples
LC auto MS/MS survey plot: A 3µg digest of human Hela cells was separated on a 50 cm column with 4 h gradient on a compact LC-MSMS system. The instrument was operated with new IDAS™ (Intensity Dependent Acquisition Speed) parent selection and acquisition strategies to obtain optimum results independent from sample complexity or amount (read Bruker App-Note LCMS-81 for more details about IDAS). This strategy enables a higher quality of acquired spectra (> 50% peptide identification rate) while the corresponding method can deliver optimum results from any samples with no adjustments.
IDAS: Illustration showing an example of time cycle handling with the Independent Data Acquisition Strategy: the number of precur-sors is chosen dynamically to maintain the constant cycle time and the MS/MS spectra rate is adapted to each precursor intensity.
400
600
800
1000
1200
1400
m/z
25 50 75 100 125 150 175 200 225 Time [min]
ID more than 2570 proteins with FDR < 1%
One Method Fitting All Samples
Sample amount 3µg 20ng
Gradient 4h 90min
ID @ 1% FDR 2575 631
IDAS
New parent acquisition / selection
Intensity Dependant Acquisition Strategy
20Hz 2Hz
MSMS spectra rate
0.00
1.00
2.00
3.00
4.00
5.00
1 2 3 4
MS MS MS MS
40 MSMS
39 MSMS
27 MSMS
30 MSMS
Aut
oMS
MS
cyc
le ti
me
[s]
AutoMSMS cycles18 19
™
Introducing new universal acquisition strategies – best results run over run
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Bru
ker
Dal
toni
cs is
con
tinua
lly im
prov
ing
its p
rodu
cts
and
rese
rves
the
rig
ht
to c
hang
e sp
ecifi
catio
ns w
ithou
t no
tice.
© B
DA
L 0
3-2
013,
181
626
0
For research use only. Not for use in diagnostic procedures.
Bruker Daltonik GmbH
Bremen · GermanyPhone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 sales@bdal.de
Bruker Daltonics Inc.
Billerica, MA · USA Fremont, CA · USAPhone +1 (978) 663-3660 Phone +1 (510) 683-4300 Fax +1 (978) 667-5993 Fax +1 (510) 490-6586 ms-sales@bdal.com ms-sales@bdal.com
www.bruker.com/compact
Source Options
Wide choice of ionization and coupling techniques for a broad range of sampling including insoluble compounds:
IonBooster source GC-MS coupling APCI II (atmospheric pressure chemical ionization) source Direct probe option for solids APPI II (atmospheric pressure photo ionization) source CE-MS coupling with grounded needle CaptiveSpray NanoElectrospray source CryoSpray source
LC Options
Bruker fully supports and integrates a wide range of leading HPLC systems, autosamplers and accessories.
GC-MS coupling
Direct probe mounted on an APCI II source
APPI II source
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