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CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
B.Sc., Microbiology
M.Sc., Microbiology
&
M.Sc., Applied Microbiology
MINUTES OF 10TH BOARD OF STUDIES, CURRICULUM,
SCHEME OF EXAMINATION AND SYLLABI (CBCS)
(2017)
1 Director, SBS
CMSCOLLEGE OF SCIENCE AND COMMERCE(Autonomous)
COIMBATORE – 641 049.
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
MINUTES OF THE 11thBOARD OF STUDIES HELD ON 12.06.2016(UG BOARD)
Recommendation made by the External Board members in the stream ofUG Microbiology.
1. The scheme for I B.Sc., Microbiology (2017-2020 batch), has been modified on the basis
of outcome based education which includes vision, mission, programme educational
objectives (PEOs), programme outcomes (POs) and mapping of PEO and POs. Syllabus
for each subject has been framed and passed with the course objectives, course outcomes
and mapping with POs for 2017-2020 batch and onwards.
2. Instructional hours for Foundation Course I and II (FC I and II) has been excluded from
the scheme and is considered as self-study paper from 2017-2020 batch onwards.
3. One hour from the instructional hours (2 hours) ofFC I and II has been incorporated in
Core Paper II – Biochemistry and increased from 4 hours to 5 hrs. One hour from the
instructional hours of FC I and II was added to Core practical I and increased from 2
hours to 3 hours in the first semester. In the second semester, 2 instructional hours of FC
I and II has been added toCore practical I and increased from 4 hours to 6 hours for the
batch 2017-2020 and onwards.
4. Modular course has been introduced for I B.Sc., Microbiology (2017-2020 batch and
onwards) and it consist of module 1 and 2. Module 1 consists of 2 papers and module 2
consists of 3 papers. 2017 batch graduates will be awarded with a certificate of diploma
in Clinical Laboratory Technology.
5. The Scheme for modular course and Syllabus of module 1 for 2017-2020 batch and
onwards is framed and passed in the meeting.
6. The syllabus of paper I and II of Module II is passed for the batch 2017 onwards. The
title of the paper III of Module II are also passed. The paper titles are as follows: Module
II – Paper I. Human Physiology, Paper II – Clinical Biochemistry, Paper III – Clinical
Microbiology.
2 Director, SBS
7. The paper Elective III of Group B – Entrepreneur Microbiology has been changed to
Bioprospecting and the paper elective III of Group C –Nanobiotechnology has been
changed to Good Laboratory Practices in the scheme for 2017-20 batch and onwards.
8. The Skill Based Subject III and IV– Clinical Laboratory Technology I and II has been
excluded and instead the papers Entrepreneur Microbiology and Bionanotechnology
respectively has been included in fifth and sixth semester in the scheme of 2017-2020
batch and onwards.
9. The syllabus for III-B.Sc., Microbiology (2015-2018 batch), II-BSc., Microbiology
(2016-19 batch) and I B.Sc., Microbiology (2017-20 batch) is passed with the below
mentioned changes.
10. The text books and the reference books has been updated for the B.Sc programs.
11. The online papers has been passed for this academic year.
The overall percentage of modifications in UG Microbiology syllabus is approximately 10 to
15%.
Inter disciplinary course in Bioinstrumentation:
An Inter Disciplinary course is offered to the 2017-2020 batch, in collaboration with the
department of Electronics, titled “Bioinstrumentation”. This can be opted by the students of
B.Sc., Biochemistry, B.Sc., Biotechnology, B.Sc., Microbiology and B.Sc., Electronics students,
in their fourth semester and will be eligible for 2 additional credits on passing the ESE in this
paper. The maximum mark for this paper is 50, which shall comprise of 40 marks for ESE and
10 marks for CIA. The CIA component shall be 5 marks for assignment and 5 marks for the
attendance.
Modular Course:
The 2017-2020 batch will be offered a modular course spread over 5 semesters, each semester
one paper will be taught for a total of 30 hours, inclusive of 8 hours of practical/field
visit/training and 2 hours of centralized examination. On successful completion of Module I (2
papers), the student will be awarded a certificate and on completion of Module II (3 papers), a
diploma will be awarded. Upon completion of 60 hours in Module – I the students are eligible to
receive “Certificate in Clinical Laboratory Technology” along with four extra credits. Upon
completion of 150 hours of Module – I & II the students are eligible to receive “Diploma
3 Director, SBS
inClinical Laboratory Technology”along with an additional six extra credits (Totally 10 credits).
Students will be eligible for credits only on completion of the entire module.
4 Director, SBS
SUGGESTIONS FOR THE MODIFICATIONS IN THE SYLLABUS FRAMED
I-B.Sc., MICROBIOLOGY (2017-20)
Core Paper I: Cell Biology
The topic “Introduction to stem cells” was added to Unit I.
Core Paper II: Biochemistry
Few topics are added in the core Biochemistry paper like structure of purines and pyrimidines etc with the
removal of Calvin cycle from unit I.
The book “Principles of Biochemistry. Lehninger, 5th Edition, W.H freeman and Company, New
York” was added as a text book instead of reference book.
Core Practical I – Cell Biology, Biochemistry and Microbiology
Addition of“Mannitol salt agar” was done in Microbiology practical.
Allied Paper I: Chemistry I – Inorganic and Physical Chemistry
In Unit-V, the topics “Radioactivity, Radioactive Decay, Measurement of Radioactivity –
Ionization - GM Counter, Excitation – Scintillation Counting” were added and the topics “Types
of Rays, Properties and Measurement (GM Counter), “An introduction to radio isotopes” were
deleted.
Allied Practical I: Chemistry
The topic “Phenol – Monohydric Phenol” was added and “Resorcinol - Polyhydric Phenol” was
deleted.
II-B.Sc., MICROBIOLOGY (2016-2019)
Core Paper IV: Microbial Taxonomy and Diversity
In Unit-I, the topic “Ecological niche” was removed and the topic is changed to” Introduction to
Evolution”.
III-B.Sc., MICROBIOLOGY (2015-2018)
Core paper X: Immunology
In Unit I, “Dentritic cells” was added.
In Unit II, “Cytokines and their functions” was included.
In Unit III, “Haemagglutination” was added.
5 Director, SBS
Online Papers for both Odd and Even Semester
Class and Batch Odd Semester Even Semester
I-B.Sc., (MB), 2017-20 Core Paper I: Cell Biology Core Paper III: Introductory
Microbiology
II-B.Sc., (MB), 2016-19
SBS I: Analytical
Biochemistry and
Biomedical Instrumentation
SBS II: Bioinformatics
III-B.Sc., (MB), 2015-18 Core Paper X: Immunology
Elective II: Environmental
and Agricultural
Microbiology
6 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE (Autonomous)
COIMBATORE – 641 049.
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
MINUTES OF THE 11thBOARD OF STUDIES HELD ON 12.06.2016(PG BOARD)
Recommendation made by the External Board members in the stream of PG Microbiology.
1. Modular course has been introduced for I-M.Sc., Microbiology and I-M.Sc., Applied
Microbiology (2017-2019 batch and onwards) and it consist of module 1, 2 and 3.
Module 1 consists of 2 papers, module 2 consists of 3 papers and module 3 consists of 3
papers. 2017 batch post-graduates will be awarded with a certificate of Post-Graduate
Diploma in Clinical Laboratory Technology.
2. The syllabus of paper I and II of Module II is passed for the batch 2017 onwards. The
title of the paper III of Module II and three papers of Module III are also passed. The
paper tiltles are as follows: Module II – Paper I. Human Physiology (SS*), Paper II –
Clinical Biochemistry (T), Paper III – Clinical Microbiology (T*). Module III – Paper I –
Endocrinology and tumour markers (T), Paper II – Immunology, Serology and
Parasitology (SS) and Paper III – Molecular Diagnostics (SS). (*SS – Self study paper, T
– Taught programme).
3. The Scheme for modular course and Syllabus of module 1 for 2017-2019 batch and
onwards is framed and passed in the meeting.
4. The papers “Research methodology and Analytical techniques” (Sem I, Elective-I) and
“Bioethics, Biosafety, TQM and IPR” (Sem-II, Core paper VIII) has been swapped.
5. 50 marks and 2 credits from “Project” was allocated to “Industrial/Institutional Training”
and made as a compulsory component of the curriculum.
6. The text books and the reference books have been updated for the PG program.
7. The online papers have been passed for this academic year.
8. The overall percentage for modification in PG Microbiology syllabus is approximately
10 to 15%
7 Director, SBS
SUGGESTIONS FOR THE MODIFICATIONS IN THE SYLLABUS FRAMED
I-M.Sc., APPLIED MICROBIOLOGY (2017-19)
Practical I: Microbial physiology, Molecular genetics and Food Microbiology
Correction of term “Turbidometric” to “Turbidimetric” and deletion of the word “production” in
“Indole production test”.
PaperIV: Molecular Genetics
In Unit-II, “Non-coding RNA – Small, long and circular” was included.
PaperVI: Industrial Microbiology
In UnitI, “(Primary and Secondary) Characteristics of industrially important microbes” was
included. Modificationof the word“SSF “to “solid state fermentation” was done in Unit-III. In
Unit-V, the topic “lipases” and “Amphotericin-B” was added.
Paper VII: Recombinant DNA Technology
In Unit II, “Northern and Southern blotting” was included.
In Unit IV, “Affimetrix®” was added.
Elective II: Bioinformatics
In Unit III, “Biopython” was included.
In Unit V, the topic “ADME properties” was added.
I-M.Sc., MICROBIOLOGY (2017-19)
PaperIV: Molecular Genetics
In Unit-II, “Non-coding RNA – Small, long and circular” was included.
Paper-V: Environmental Microbiology
In Unit-I, the topics “virus and helminthes” was deleted and the topic “Temperature” was
included. In Unit-II, the topic “gaseous emission” and “their symptoms” were deleted. The
topics “Algae, Phytoplankton”, “Drinking water – Potability” was deleted from Unit-III and
“Potability of Drinking water – Quality standards for drinking water” was included. In Unit-IV,
rearrangement of“Types and sources of solid and liquid wastes” was done from “solid and liquid
waste”.
8 Director, SBS
Paper VII: Recombinant DNA Technology
In Unit II, “Northern and Southern blotting” was included.
In Unit IV, “Affimetrix®” was added.
Elective-II: Bioinformatics
In Unit III, “Biopython” was included.
In Unit-V, the topic “ADME properties” was added.
II-M.Sc., APPLIED MICROBIOLOGY (2016-18)
Paper XI: Immunology and Immunotechnology
In Unit II, “Definition and Classification of Exo and Endo toxins”
In Unit IV, “Immuno–suppressants” was changed as “Immunosuppressants”
In Unit V, “Immuno–technology” was changed as “Immunotechnology”, “immune–diffusion”
was rewritten as “immunodiffusion”, “Immuno–fluorescent”, was written as
“Immunofluorescent” and “immune–electron” was written as “immunoelectron”.
“Immunohistochemistry – an overview” was included.
Paper XII: Genomics and Proteomics
In Unit I, “NGS (Next Generation Sequencing)” was added.
In Unit-II, inclusion of“Hidden Markov Model” was done.
Inclusion of“Secondary structure prediction: Chou – Fasman / GOR method, Neural network”
was done in Unit-IV.
In Unit V, “MALDI-TOF and its variants” was included.
II-M.Sc., MICROBIOLOGY (2016-18)
Paper XI: Immunology and Immunotechnology
In Unit II, “Definition and Classification of Exo and Endo toxins”
In Unit IV, “Immuno–suppressants” was changed as “Immunosuppressants”
In Unit V, “Immuno–technology” was changed as “Immunotechnology”, “immune–diffusion”
was rewritten as “immunodiffusion”, “Immuno–fluorescent”, was written as
9 Director, SBS
“Immunofluorescent” and “immune–electron” was written as “immunoelectron”.
“Immunohistochemistry – an overview” was included.
Paper XII: Genomics and Proteomics
In Unit I, “NGS (Next Generation Sequencing)” was added.
In Unit-II, inclusion of “Hidden Markov Model” was done.
Inclusion of “Secondary structure prediction: Chou – Fasman / GOR method, Neural network”
was done in Unit-IV.
In Unit V, “MALDI-TOF and its variants” was included.
The overall percentage of modifications in PG Microbiology syllabus is approximately 10 to
15%.
Online Papers for both Odd and Even Semester
Class and Batch Odd Semester Even Semester
I-M.Sc., (MB), 2017-19
Elective I: Research
Methodology and
Bioanalytical techniques
Paper VIII: Bioethics,
Biosafety, TQM and IPR
I-M.Sc., (AMB), 2017-19
Elective I: Research
Methodology and
Bioanalytical techniques
Paper VIII: Bioethics,
Biosafety, TQM and IPR
II-M.Sc., (MB), 2016-18 Paper XI: Immunology and
Immunotechnology
Paper VIII: Soil and
Agricultural Microbiology
II-M.Sc., (AMB), 2016-18 Paper XI: Immunology and
Immunotechnology
Paper VIII: Environmental
and Agricultural
Microbiology
10 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
I Year B.Sc., MICROBIOLOGY
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS) (2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017)
11 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.2
DEPARTMENT OF MICROBIOLOGY
VISION OF THE DEPARTMENT
To produce competent professionals in every field of Microbiology by providing inspirational
learning process to complement our national needs and to be recognized as a Centre of
Excellence in Research, Innovation, Consultancy and Entrepreneurship.
MISSION OF THE DEPARTMENT
To provide facilities and offer opportunities for graduate and post-graduate education in
microbiology through advanced instruction/teaching methodologies, quality training and
innovative research;
To prosecute and promote research in microbiology for the advancement of knowledge
and for the betterment of society.
To serve as a centre for fostering co-operation and exchange of ideas between the
academic, research and industrial sector.
B.Sc., MICROBIOLOGY COURSE
PROGRAM EDUCATIONAL OBJECTIVES
PEO 1: To acquire basic knowledge on core concepts in diverse field of Microbiology.
PEO 2: To inculcate practical training on basic techniques and processes in Microbiology.
PEO 3: To impart skill-based education for entrepreneurial development.
PEO 4: To adapt with the continuously evolving technologies in industry.
After completion of the program the graduates will be able to
PO1: Execute the knowledge in identifying problems and to develop a solution.
PO2: Apply/improve analytical and technical skills to enhance employment potential.
PO3: Implement the skill set to become a successful entrepreneur.
PO4: Acquire a holistic professional career in hospitals, food and pharmaceutical industries.
12 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.3
MAPPING OF PEO’S AND PO’S
PEO1 PEO2 PEO3 PEO4
PO1
PO2
PO3
PO4
DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT No of
Papers
Marks
@ Credits
I Language – I Tamil /Malayalam/Hindi/French 4 400 16
II Language – II English 4 400 16
III
Core Subjects 16 $ 1500 60
Allied Subjects 6** 400 16
Elective Subjects 3 300 12
Practicum - 50 2
IV
1 - Non-Major Elective – I
Basic Tamil₤/Women’s Rights
2- Non-Major Elective – II
Advanced Tamil₤/General Awareness
2 100 # 4
2 – Skill Based Subjects 4 200 8
3 – Foundation Course I 1 50 # 2
4 – Foundation Course II 1 50 # 2
V Extension Activities - 50!! 2
VI Library - - -
Total 3500 140
13 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.4
Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects
respectively.
$ In core subjects both theory and practical should be included wherever applicable.
** In allied subjects both theory and practical should be included wherever applicable.
# No continuous internal assessment for these subjects.
!! The evaluation of extension activities will be based on NSS/ NCC/ SPORTS/ Red Cross.
₤ Continuous internal assessment for these subjects.
∞ Foundation Course I and II are included as self study
The following parameters are considered throughout study period.
i) Regularity of attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other social components (Blood camp, Fine arts etc)
Note: II
The Credit points and Lecture Hours are not linked.
14 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.5
Annex: UG MB
BOS DT: 12:06:2017
B.Sc., MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017)
Sem
este
r
Par
t
Su
bje
ct c
ode
Subject Tile
Inst
. H
rs p
er w
eek
Examination Details
Cre
dit
s
Du
rati
on
hou
rs
CIA
En
d S
emes
ter
Exa
m
Tot
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ark
s
SEMESTER I
I
I Language I 6 3 25 75 100 4
II English I 6 3 25 75 100 4
III Core Paper I – Cell Biology 4 3 25 75 100 4
III Core Paper II – Biochemistry 5 3 25 75 100 4
III Core Practical I – Cell Biology,
Biochemistry and Microbiology 3 - - - - -
III
Allied Paper I – Chemistry I:
Inorganic and Physical
Chemistry
4 3 20 55 75 3
III Allied Practical I – Chemistry 2 - - - - -
IV Foundation Course I –
Environmental Studies - 3 - 50 50 2
Total 30 - - - 525 21
SEMESTER II
II
I Language II 6 3 25 75 100 4
II English II 6 3 25 75 100 4
III Core Paper III – Introductory
Microbiology 5 3 25 75 100 4
15 Director, SBS
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Page No.6
III Core Practical I: Cell Biology,
Biochemistry and Microbiology 6 3 40 60 100 4
III Allied Paper II – Chemistry II:
Organic Chemistry 4 3 20 55 75 3
III Allied Practical I – Chemistry 3 3 20 30 50 2
IV Foundation Course II – Ethics
and culture - 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER III
III
I Language III 6 3 25 75 100 4
II English III 6 3 25 75 100 4
III Core Paper IV – Microbial
Taxonomy and Diversity 4 3 25 75 100 4
III Core paper V – Microbial
Physiology 4 3 25 75 100 4
III
Core Practical II – Molecular
Genetics and Microbial
Physiology
2 - - - - -
III Allied paper III – Basic
Mathematics 3 3 20 55 75 3
IV
Skill based Subject 1 –
Analytical Biochemistry and
Biomedical Instrumentation
3 3 10 40 50 2
IV Non-major elective I – Basic
Tamil / Women’s Rights 2 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER IV
IV I Language – IV 6 3 25 75 100 4
II English IV 6 3 25 75 100 4
16 Director, SBS
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Page No.7
III Core Paper VI – Molecular
Genetics 4 3 25 75 100 4
III
Core Practical II – Molecular
Genetics and Microbial
Physiology
4 6 40 60 100 4
III
Allied Paper IV –
Fundamentals of Computers
& C Programming
3 3 20 55 75 3
III
Allied Practical II –
Fundamentals of Computers
& C Programming
2 3 20 30 50 2
IV Skill based Subject 2 –
Bioinformatics 3 3 10 40 50 2
IV Non-major elective II –
General Awareness 2 3 - 50 50 2
Total 30 - - - 625 25
SEMESTER V
V
III Core Paper VII – Medical
Microbiology-I 4 3 25 75 100 4
III Core Paper VIII – Genetic
Engineering 5 3 25 75 100 4
III Core Paper IX – Virology 5 3 25 75 100 4
III Core Paper X – Immunology 4 3 25 75 100 4
III Elective – I 4 3 25 75 100 4
IV Skill based Subject 3 –
Entrepreneur Microbiology 3 3 10 40 50 2
III
Core Practical III – Medical
Microbiology, Industrial and
Agricultural Microbiology
5 - - - - -
III Practicum* - - 20 30 50 2
Total 30 - - - 600 24
17 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.8
SEMESTER VI
VI
III Core Paper XI – Medical
Microbiology II 5 3 25 75 100 4
III Core Paper XII – Industrial
Microbiology 5 3 25 75 100 4
III
Core Practical III – Medical
Microbiology, Industrial and
Agricultural Microbiology
6 9 40 60 100 4
III Elective – II 5 3 25 75 100 4
III Elective – III 5 3 25 75 100 4
IV Skill based Subject 4 – Bio
nanotechnology 3 3 10 40 50 2
V Extension Activities - - 50 - 50 2
VI Library 1 - - - - -
Total 30 - - - 600 24
Grand total - - - - 3500 140
Practicum:
Each student has to undergo an Industrial / Institutional training during the summer vacation
prior to Semester V. This should be of a minimum duration of 15 working days at any off-
campus site approved by the Director. Students are required to submit three copies of
Practicum report with attendance certificate upon completion and the bonafide report carried
out by the candidate under the guidance of a faculty authenticated and countersigned by the
Director. This practicum must be presented by the candidate in the department (at the end of
fifth semester) attended by all faculty and reviewed by the respective guide as examiner.
@ Practicum:
Attendance and internal assessment by the guide = 20 marks (Internal)
Final Viva Voce = 30 marks (External) (Dissertation = 20 marks + Viva = 10 marks)
Total Credits: 140 [Part I -16, Part II -16, Part III-90, Part IV -16, Part V-2]
18 Director, SBS
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Page No.9
Each paper carries an internal component; there is a pass minimum for External component
and overall.
Distribution of marks between Internal* and External assessment
Theory: Internal assessment (25)*: External assessment (75)
Practical: Internal assessment (40)*: External assessment (60)
COMPONENTS OF INTERNAL ASSESSMENT
Theory (for 25 marks): One Internal test and One Model test in each semester
Internal Test: 05 marks*
End semester Model test: 10 marks
One Assignment: 05 marks
Overall performance: 05 marks
Allied/ SBS(for 20 marks): One Internal test and One Model test in each semester
Internal Test: 04 marks*
End semester Model test: 08 marks
One Assignment: 04 marks
Overall performance: 04marks
Core Practical I, II: (for 40 marks):
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Observation: 05 marks
Core Practical III:
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Allied: (20 marks)
Internal Test: 04 marks*
End semester Model test: 08marks
19 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.10
One Assignment: 04 marks
Overall performance: 04 marks
SBS: (10 marks)
Internal Test: 02 marks*
End semester Model test: 04 marks
One Assignment: 02 marks
Overall performance: 02 marks
*Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge and subject in charge. The final decision to be made by
the Director.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I Food and Dairy Microbiology
Parasitology Biomolecules
Paper II Environmental and
Agricultural Microbiology
Medical Biochemistry Plant Biotechnology
Paper III
Pharmaceutical Microbiology
Bio prospecting Good Laboratory
Practices
20 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.11
Annex EDC: UG MB
BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE AND COMMERCE, (AUTONOMOUS)
COIMBATORE – 641 049.
B. Sc., Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2014 onwards)
Note:
The student has to compulsorily select one Extra Disciplinary Course offered by other
departments and are eligible to get 2 extra credits. This paper is offered as self-study
from the 2014 batch onwards.
Par
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Examinations
Du
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Semester V
VI Mushroom cultivation - 3 10 40 50 2
Total - - - - 50 2
21 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.12
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049.
B. Sc., MICROBIOLOGY
MODULAR COURSE
Diploma in Clinical Laboratory Technology (Diploma in CLT)
(For the students admitted during the academic year 2017 onwards)
Note:
The student has to undergo compulsorily a Diploma course offered by the department
and the student is eligible to get 2 extra credits for each module. This diploma course
consists of module 1 and 2. Module 1 consists of 2 papers and Module 2 consists of 3
papers. Total credits of the modular course are 10. After the successful completion of
the Module I and II, students are eligible to get a Diploma in Clinical Laboratory
Technology
Minimum 10 days of clinical laboratory training is compulsory during IIIrd year.
Par
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Du
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Module I – Paper I – Introduction to Clinical Laboratory Technology
30 90
mins 10 40 50 2
Module I – Paper II – Techniques in Clinical Laboratory Technology
30 90
mins 10 40 50 2
Module II – Paper I - Human Physiology
30 90
mins 10 40 50 2
Module II – Paper II – Clinical Biochemistry
30 90
mins 10 40 50 2
Module II – Paper III – Clinical Microbiology
30 90
mins 10 40 50 2
Total 150 50 200 250 10
22 Director, SBS
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Page No.13
Year Odd semester Even semester Module details
I - 1st paper Module 1
II 2nd paper (Module 1) 1st paper (Module 2) Module 1 and 2
III 2nd paper (module 2) 3rd paper (Module 2) Module 2
23 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.14
SEMESTER – I
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper I – Cell Biology
NUMBER OF HOURS/WEEK: 4 Hrs
COURSE OBJECTIVE
Understanding the structural and functional aspects of the cell, provide the student with a
strong foundation in the molecular mechanisms underlying cellular functions.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the structural and functional aspects of the cell (K2)
CO2: Recognize the role of different outer structural membranes (K1)
CO3: Examine the characteristics of cellular organelles and interpret how it is involved in cell
activity (K4)
CO4: Discover the similarities and dissimilarities between the organelles having its own DNA
(K3)
CO5: Interpret the characteristics of genetic material, how it flows from one generation to
another in different life forms and understand the diseases caused by mistakes in the flow (K4)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 25 ESE – 75
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CONTENTS
UNIT-I
Origin and evolution of life. Early chemical investigation in cell biology, cell theory, Types of
tissues, Classification of cells: Prokaryotic and eukaryotic cells, and their comparison.
Comparison of microbial, plant, and animal cells. Introduction to stem cells.
UNIT-II
Structure and function of cell wall, plasma membrane, capsule, slime layer, flagella and pili,
photosynthetic apparatus, endospore and inclusion bodies. Transport across membranes:
Diffusion, active and passive transport and ion channels.
UNIT-III
Structure and function of Endoplasmic reticulum, Golgi apparatus, Ribosomes, Lysosomes,
Peroxisomes and Glyoxysomes, Gas vacuoles. Cell appendages – Cilia.
UNIT-IV
Structure and function of Mitochondria, Chloroplast – an overview of photosynthesis in
plants. Cytoskeleton: Types – Microfilaments, Intermediate filaments and Microtubules.
UNIT-V
Structure and function of nucleus and chromosomes, cell cycle: Phases of cell cycle, cell
division in prokaryotes and eukaryotes; mitosis and meiosis; cell death - apoptosis and
necrosis. Introduction to cancer cells, an overview of growth, phases and types of cancer cells.
TEXT BOOKS:
1. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology– P.S. Verma& VK
Agarwal, 2004 S. First muti colour edition (2004), publisher Chand &Ltd., New Delhi
2. Molecular Cell Biology- Harvey Lodish, Arnold Berk, Paul Matsudaira, Chris A. Kaiser,
Monty Krieger, Matthew P. Scott, Lawrence Zipursky and James Darnell,5th Edition (2000),
published by W.H Freeman and Company, New York.
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REFERENCE BOOKS:
1. Cell and Molecular Biology - Gerald Karp 3rd Edition (2002),Publisher John Wiley and
Sons, New York.
2. Molecular Biology of the Gene -Watson, Baker, Bell, Gann, Levine, Losick, 5thEdition
(2005), Published by Pearson Education Pvt Ltd., Singapore.
3. Cell and Molecular biology – De Robertis, 8thEdition, (2006), B.J. Publications Pvt Ltd.
4. The Cell – Geoffery M. Cooper, 4 th edition, (2007), ASM, Washington DC, U.S.A.
PEDAGOGY: Chalk and Talk, OHP and PPT
26 Director, SBS
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Page No.17
SEMESTER – I
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper II : Biochemistry
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge about the structure of Biomolecules, understand basic cellular
metabolism, and to enable the students to elucidate the role of biomolecules in the functioning
of the organism.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1.Understand the structure and significance of carbohydrates (k2).
CO2. Recognize differences between lipid types (k2).
CO3. Analyze proteins based on their conformation (k4).
CO4. Evaluate the nature and action of enzymes (k5).
CO5. Predict the structure and metabolism of nucleic acids (k3).
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CONTENTS:
UNIT I
Carbohydrates – Classification, structure and biological significance of carbohydrates; an
overview of carbohydrate metabolism: Glycolysis, TCA Cycle, Gluconeogenesis, Glycogen
breakdown and synthesis. Energy metabolism: Oxidative phosphorylation, electron transport
chain and Photophosphorylation.
CIA – 25 ESE – 75
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UNIT II
Lipids and Vitamins: Structure, function and classification of lipids; An overview of Lipid
metabolism: Biosynthesis of fatty acids, Triglycerides and Phospholipids, Degradation of fatty
acids: β oxidation. Vitamins – types and their biological importance.
UNIT III
Amino acids and proteins: Classification and structure of amino acids. Proteins: Structure of
peptide bond, Classification of proteins, Primary, secondary, tertiary and quaternary structure
of proteins.
UNIT IV
Enzymes – Classification and nomenclature. Enzyme specificity and factors affecting enzyme
activity. Enzyme kinetics: Michaelis - Menten equation (derivation not required), significance
of Km and Vmax. Allosteric enzymes, Coenzymes, Cofactors. Enzyme inhibition – Types and
mechanism. An overview of isoenzymes, ribozymes and abzymes.
UNIT V
Nucleic Acids: Structure and classification of Nucleic acids- structure of purines and
pyrimidines, Nucleotides, DNA double helical structure and different forms of DNA (A, B
and Z- DNA), RNA- Types and functions. An overview of nucleic acid metabolism:
Biosynthesis of purines and pyrimidines (de novo and salvage pathways). Degradation of
Purines and Pyrimidines.
REFERENCES:
TEXT BOOKS:
1. Fundamentals of Biochemistry - Jain, J.L. (2004) S. Chand & Company, Limited
2. Fundamentals of Biochemistry- Deb. A. C. (2001) New Central Book Agency (P)
Ltd.
3. Essentials of Biochemistry – U. Satyanarayana, 4th Ed. (2014) Books and Allied
Publications, Kolkata.
REFERENCE BOOKS:
1. Principles of Biochemistry – Smith et al., 8th Ed. (1983) McGraw Hill International
book company 8th Ed.
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Page No.19
2. Outlines of Biochemistry – Conn. E. & Stump. P.K., 5th Ed. (2009), John Willey &
Sons, New Delhi.
3. Lehninger Principles of Biochemistry - David L. Nelson, Michael M. Cox, 7th
Edition (2017), W.H freeman and Company, New York.
4. Biochemistry - Jeremy M. Berg, John L. Tynoczko, Lubert Stryer, 3rd Edition (2015),
W.H. Freeman and Company, New York.
5. Harpers Illustrated Biochemistry- Robert K. Murray, Drayl. K. Granner, Peter. A.
Victor. W. Rodwell, 30th Edition (2015), Tata McGraw-Hill companies.
PEDAGOGY: Chalk and Talk, OHP, PPT.
29 Director, SBS
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Page No.20
SEMESTER – I
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Practical I – Cell Biology, Biochemistry and Microbiology
NUMBER OF HOURS/WEEK: 3 Hrs
COURSE OBJECTIVE
To demonstrate the laboratory safety
To learn and understand the principles behind the qualitative estimation of bio
molecules (carbohydrates and amino acids)
To provide the fundamental knowledge on cell biology.
To learn about the morphology and characteristics of microbes using the techniques.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate the working principles of instruments. (K3)
CO2: Understand the concept of buffer and preparation. (K2)
CO3: Carry out qualitative and quantitative analysis of bio molecules. (K4)
CO4: Differentiate the microbes using different staining techniques. (K4)
CO5: Acquire knowledge on qualitative and quantitative techniques in microbiology. (K2)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 40 ESE – 60
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Page No.21
CONTENTS
General:
1. Introduction to Bioinstrumentation – Principle, types and applications of -
Colorimeter, pH meter, Microscopy, Laminar air flow, Incubator and centrifuge.
2. Preparation of buffers- Acetate Buffer and Phosphate buffer.
Cell Biology:
3. Cell types- Plant and Animal cells.
4. Cell fractionation– Demonstration.
Biochemistry:
5. Qualitative Analysis:
a) Carbohydrates
i) Monosaccharides: Glucose
ii) Disaccharides: Sucrose
iii) Polysaccharides: Starch
b) Amino Acids: Glycine, Tyrosine, Tryptophan, Cysteine and Arginine (any two).
6. Estimation of Glucose by Benedict’s method.
7. Estimation of Protein by Lowry’s method.
8. Estimation of DNA by Diphenylamine method.
9. Estimation of RNA by Orcinol method.
Microbiology:
10. Laboratory precautions.
11. Demonstration of methods of sterilization.
12. Sterility testing – Phenol coefficient test.
13. Culture media preparation – Liquid and solid medium.
14. Preparation of selective and differential medium – Mannitol Salt agar, EMB and
McConkey agar.
15. Enumeration of bacteria from soil.
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16. Pure culture method-pour plate, spread and loop method.
17. Staining of bacteria-simple, Gram, spore and negative.
18. Staining of fungi – Lactophenol cotton blue.
19. Motility test – hanging drop and soft agar inoculation.
20. Cultural characteristics of microorganisms – colony morphology on plate, broth and
slant.
REFERENCE BOOKS:
BIOCHEMISTRY
1. Practical Biochemistry – Principles and Techniques. Keith Wilson and John Walker
(2000). 2nd Edition. Cambridge University Press.
2. Laboratory Manual in Biochemistry. Jayaraman J., (1996). Wiley Eastern Limited.
3. Biochemical Methods. Sadasivam. S. and Manickam. A. (2004). 2nd Edition., New
Age International Ltd Publishers, New Delhi.
4. An Introduction to Practical Biochemistry. David.T. Plummer. (1987). 3rd Edition.
McGraw-Hill Book Company (U.K.) Ltd., London.
5. Biochemical Methods. Sadhasivam and A. Manickam. 2nd Edition. New Age
International Publishers.
MICROBIOLOGY
1. Practical Microbiology. R.C. Dubey.
2. Laboratory Manual in Microbiology. Dr. N.Kannan.
3. Microbiology: A Laboratory Manual. James G. Cappuccino and Natalie Sherman
(1987). 2nd Edition. Pearson/Benjamin Cummings (San Francisco).
4. Experiments in Microbiology. K. R. Aneja, (2005). 4th Edition, New Age
International Ltd Publishers, New Delhi.
PEDAGOGY: Emphasis will be given to systematic designing of experiments in the
laboratory sessions.
32 Director, SBS
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Page No.23
SEMESTER – I
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Allied Paper I: Chemistry I – Inorganic and Physical Chemistry
NUMBER OF HOURS/WEEK: 4 Hrs
COURSE OBJECTIVE
To establish the role of inorganic and physical chemistry in the biological sciences
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Analyze an atom or ion of a given element providing the full electronic configuration
and to define electronegativity, electron affinity and ionization potential (K4)
CO2: Distinguish whether a bond is metallic, ionic, covalent or polar covalent (K4)
CO3: Understanding the basic concepts of thermodynamics and kinetics (K2)
CO4: Outline core concepts of solubility and dissociation in water and apply this knowledge
to acids, bases and salts and to identify weak and strong acids and bases (K4)
CO5: Recall the nuclear decay including applications to radioactive dating (K1)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 20 ESE – 55
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Page No.24
CONTENTS
UNIT-I
Structure of an atom: Bohr’s model of an atom, energy of electron, dual character of
electrons, Debroglie’s equation. Heisenburg’s uncertainty principle, Quantum numbers,
Pauli’s exclusion principle, filling of various orbitals in atoms, Electronic configuration of
elements, Chemical periodicity, Atomic properties– Atomic radius, Covalent radius, Vander
waal’s radius, Ionic radius, Ionization energy, Electron affinity, Electronegativity.
UNIT-II
Chemical Bonding: Ionic bond, Covalent bond, Hydrogen bonding, Bond energy, Valence
bond theory, Orbital overlapping σ and π bonds, Hybridization (Sp, Sp2 and Sp3), Bond length.
VSEPR theory – Shape of CH4, C2H2, H2O, NH4. MO Theory – Bonding and Anti-bonding
orbitals – energy level diagrams. Comparison between VB and MO Theory.
UNIT-III
Chemical kinetics: Introduction, Rate of reactions, factors influencing rates of reaction.
Various order reaction – Zero order, First order, Second order and third order reaction
(Overview and an example each).
Thermodynamics: Introduction, Terminology – Isolated, Closed and Open systems. Process
– Isothermal, Adiabatic, Reversible and Irreversible process. Laws of Thermodynamics
(statement only). Joule Thomson effect. Concept of Entropy and Enthalpy, Free energy
changes, Gibb’s Duhem equation.
UNIT-IV
Chemical Equilibrium: Acids and Bases – Arhenius concept, Proton transfer theory,
Conjugate acids and bases, Strength of acid-base pairs, Dissociation of weak acids and bases,
Dissociation of water. pH and pH scale, Calculation of pH of buffers. Henderson-Hasselbalch
Equation . Measurement of pH – pH paper and pH meter – glass electrode, standard hydrogen
electrode, reference electrode. Acid-base titrations and use of indicators, titration of strong
acid against a strong base and titration of a weak acid with a strong base.
UNIT-V
Various ways of expression and conversion of concentration of solutions - molality,
molarity, normality, mole fraction, parts per million and percentage solutions (Simple
problems to be worked out).
34 Director, SBS
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Page No.25
Photochemistry: Introduction, Consequences of light absorption (Phosphorescence and
Fluorescence). Light absorption by solutions – the Beer-Lambert law and its limitations.
Photochemical reactions– Rhodopsin cycle. Energy transfer in photochemical reactions,
photosensitization and Quenching. Chemiluminescence and LASER.
TEXTBOOKS:
1. Text Book of Inorganic Chemistry, P.L.Soni, Mohan Katyal, Sultan Chand & Sons
publications (2013)
REFERENCE BOOKS:
1. The Basics of Chemistry- Richard Meyers (2003) Greenwood press
2. Inorganic Chemistry- Puri BR and Sharma LR. Shobanlal Nagin Chand & Co, Jalandar
city, Delhi.
3. Physical Chemistry-David W Ball (2003) Cengage Learning India Pvt Ltd, New Delhi.
PEDAGOGY: Chalk and Talk, OHP, PPT
35 Director, SBS
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Page No.26
SEMESTER – I
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Allied Practical I: Chemistry
NUMBER OF HOURS/WEEK: 2 Hrs
COURSE OBJECTIVE
To provide an experimental basis of chemistry in understanding the core principles of
biological compound and to give the students the practical experience in preparing and
determining the strength of the acids and bases.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the concepts of expressing the concentration of the solution. (K2)
CO2: Prepare standard solutions. (K6)
CO3: Estimate the strength of a given solution. (K5)
CO4: Analyze the basic structures of organic compound. (K4)
CO5: Report the special characteristics of organic compounds. (K2)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 20 ESE – 30
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Page No.27
CONTENTS
VOLUMETRIC ANALYSIS
1. Estimation of Sodium hydroxide
2. Estimation of Hydrochloric acid
3. Estimation of Oxalic acid (using NaOH)
4. Estimation of Potassium permanganate
5. Estimation of Oxalic acid (using KMnO4)
6. Estimation of Ferrous sulphate
QUALITATIVE ANALYSIS
Analysis of Organic Compound
1. Carbohydrate
2. Mono Carboxylic acid
3. Di- Carboxylic acid
4. Resorcinol - Polyhydric Phenol
5. Primary amine
6. Diamide
REFERENCES
1. Elementary practical organic chemistry – Part III Quantitative organic analysis
Arthur Vogel. (1970). 6th Edition. Longman Group Limited.
2. Practical organic chemistry. Julius B. Cohen, (1908). 2nd Edition. Macmillan and Co
ltd.
PEDAGOGY: Chalk and talk, and Demonstration of experiments.
37 Director, SBS
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Page No.28
SEMESTER – II
COURSE NAME: B.Sc., Microbiology
SUBJECT TITLE: Core Paper III: Introductory Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
Introduce an overview of microorganism. Execute general concepts concerning the
morphology, staining and sterilization techniques.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the scope and historical developments in microbiology. (K2)
CO2: Analyze the principles of sterilization and disinfection techniques. (K4)
CO3: Explore the different cultural techniques used in microbiology. (K3)
CO4: Understand the principle of microscopy and the different types of microscopes used. (K2)
CO5: Understand the principle of different staining techniques. (K2)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 25 ESE – 75
38 Director, SBS
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Page No.29
CONTENTS
UNIT-I
Historical development of Microbiology - Biogenesis vs abiogenesis. contribution of John
Needham, LazzaroSpallanzani, Theodar Schwann, John Tyndall, Joseph Lister, Antonie van
Leeuwenhoek, Louis Pasteur, Robert Koch, Winogradsky, Beijerinck, Edward Jenner and
Ananda Mohan Chakraboraty. Scope of Microbiology.
UNIT-II
Sterilization and disinfection – Principles – methods of sterilization– dry heat, moist heat,
filtration, Radiation, Pasteurisation, Tyndallization, Chemical sterilization- Chemical agents:
mode of action (Phenol, detergents, Aldehydes, Gaseous agents). Phenol coefficient test –
Sterility testing.
UNIT-III
Culture techniques- Shake culture – Enrichment culture – Roll tube & stab culture, agar
slant, agar deep, broth culture. Types of medium: selective, differential, enrichment, enriched,
transport and anaerobic media Assay medium, enumeration of microorganisms- Dilution
techniques, plate count, cell mass, direct count – hemocytometer.
UNIT-IV
Microscopy – Principles and components of Microscope- Simple and compound microscope-
Dark field, phase contrast – Fluorescence and electron microscopy-TEM and SEM.
UNIT-V
Principles of staining – Stains, Dyes, Acidic and basic – Simple, Differential, Negative, Spore,
Flagella, Nucleic acids and Acid fast staining.
TEXT BOOKS:
1. Microbiology, Concepts and Applications. Michael J. Pelczar. Jr. (1993). McGraw-
Hill. Inc.
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Page No.30
REFERENCE BOOKS:
1. Microbiology. Prescott, L.M; J.P. Harley and D. A. Klein. (1993). 2nd edition. WMC
Brown publishers.
2. Biology of Microorganisms. Brock, TD. Smith D.W. and Madigan M.T. (1984). 4th
edition. Englewood, cliffs. N.J. Prentice Hall.
3. Microbiology- Fundamentals and Applications. Ronald M. Atlas. (1989). 2nd
edition. Maxwell Macmillan international editions.
PEDOGOGY: Chalk and Talk and PPT
40 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.31
SEMESTER – II
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Practical I – Cell Biology, Biochemistry and Microbiology
NUMBER OF HOURS/WEEK: 6 Hrs
Refer semester I for Core Practical I syllabus
CIA – 40 ESE – 60
41 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.32
SEMESTER – II
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Allied Paper II – Chemistry II: Organic Chemistry
NUMBER OF HOURS/WEEK: 4 Hrs
COURSE OBJECTIVE
Understand the structural, properties, stereochemistry and reaction mechanisms of carbon
based molecules.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand the basic concepts and purification methods of organic compounds (K2)
CO2: Categorize the types of reactions and mechanisms involved (K4)
CO3: Discriminate the types of isomerism and relate its use in organic compounds (K5)
CO4: Discover the characteristics of few organic compounds and its reactions (K3)
CO5: Compare different groups of organic compounds and interpret its characteristics (K5)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 20 ESE – 55
42 Director, SBS
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Page No.33
CONTENTS
UNIT-I
Organic chemistry: Definition and importance, Sources of organic compounds, Purification
of organic compounds – Crystallization, Sublimation, Distillation and Fractional Distillation,
Distillation under reduced pressure, Steam distillation, Extraction with solvents and
Chromatography. Test for purity, Detection of elements – Carbon, Hydrogen, Oxygen,
Nitrogen, Halogen and Sulphur. Polarity of solvents.
UNIT-II
Classification of Organic reagents: Types of reactions – Substitution, Addition, Elimination
and Rearrangement reactions. Mechanism of substitution reaction: Free radical, Electrophilic
and Nucleophilic substitution reaction – SN1 and SN2 reactions. Mechanism of elimination
reaction – E1 and E2 reactions. Mechanism of Addition reaction – Electrophilic and
Nucleophilic addition reactions.
UNIT-III
Isomerism: Definition, Types – Structural isomerism (Chain and Position isomerism),
Functional isomerism, Metamerism, Tautomerism, Stereoisomerism – Geometric and Optical
isomerism – Asymmetric Carbon, Enantiomers, Diastereomers, Racemization, R S system for
asymmetric molecules, E-Z system for geometric isomers.
UNIT-IV
Phenol: Structure, preparation (any two), physical properties, chemical properties –
Formation of salts, reaction with FeCl3, Halogenation, Nitration, Sulphonation, Reaction with
pthalic anhydride. Chemical properties of phenols of biological origin- catechol, pyrogallol
and phloroglucinol. Applications of phenol.
Benzoic acid: Structure, preparation (any 2), physical properties, chemical properties –
Reaction with Sodium hydroxide, Sodium bicarbonate, Ester formation and Decarboxylation.
Applications of Benzoic acid.
Aniline: Structure, preparation (any 2), physical properties, chemical properties – Reaction
with Hydrochloric acid, Acetylation, Halogenation, Sulphonation, Nitration, Reaction with
Nitrous acid, Carbylamine reaction. Applications of Aniline.
Urea: Structure, preparation (any 2), physical properties, chemical properties – Hydrolysis,
Reaction with Nitrous acid, Biuret test. Applications of Urea.
43 Director, SBS
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UNIT-V
Alkaloids: Introduction, Classification and its Occurrence and Extraction. Piperine –
Structure, Physio-Chemical properties and Biological applications.
Terpenes: Introduction and Classification. Geraniol - Structure, Physical and Chemical
Properties.
Heterocyclic compounds: Aromatic heterocyclic compounds – Furan – preparation (any
two), properties – physical and chemical – nitration and sulfonation, reduction, halogenation,
uses. Pyridine – isolation from coal tar, properties – physical and chemical – halogenation,
nitration and sulfonation, reduction, uses. Quinoline – preparation (SKRAUP synthesis),
properties – physical and chemical – nitration, bromination, oxidation, reduction and uses.
REFERENCES
TEXTBOOK:
1. Text Book of Organic Chemistry, P.L.Soni, H.M. Chawla, Sultan Chand & Sons
publications Twenty ninth edition (2013)
REFERENCE BOOKS:
1. General chemistry by Kotz et al., (2009) Cengage Learning India Pvt Ltd, New Delhi.
2. Organic Chemistry by Bhupinder Mehta and Manju Mehta (2010) PHI Learning Pvt Ltd.
PEDAGOGY: Chalk and Talk, PPT and OHP.
44 Director, SBS
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Page No.35
SEMESTER – II
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Allied Practical I: Chemistry
NUMBER OF HOURS/WEEK: 3 Hrs
Refer semester I for Allied chemistry practical I syllabus
CIA – 20 ESE – 30
45 Director, SBS
Anx.UMb11,1B.Sc. Mb (CMS College) 2017 2017-20 batch
Page No.36
SEMESTER – II
MODULAR COURSE-MODULE 1
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Paper I: Introduction to Clinical Laboratory Technology
NUMBER OF HOURS/WEEK: 30 Hrs
COURSE OBJECTIVE
To gain the knowledge on the clinical assessment of various diseased conditions & Routine
test followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: To understand the basics of clinical laboratory (K2)
CO2: To understand preparation of reagents and its storage (K2)
CO3: Understand the importance of biological samples (K2)
CO4: To interpret and report clinical tests (K4)
CO5: To categorize and disposal of biomedical wastes (K6)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 10 ESE – 40
46 Director, SBS
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Page No.37
CONTENTS
UNIT-I
Basic laboratory principles - Code of conduct of medical laboratory personnel.The use of the
laboratory - Basic laboratory principles - Organization of Laboratory Functional components
of clinical laboratories (Cleanliness – Methods of hand wash, Cleaning of lab instruments,
Laboratory floors and table tops, precautions to be taken WRT patients, reports, analysis).
Basic needs of clinical laboratory technician, awareness of soft skills. Safety measures -
Medical laboratory professional and professionalism in laboratory workers - clinic borne
infection and personnel hygiene.
UNIT-II
Reagent preparation: preparation of molar, normal, percent solutions. Buffers, Acid, Base
solutions and its storage. Common Laboratory Equipment’s use, principle and maintenance-
spectrophotometer, Incubator, Hot Air Oven, Water Bath Centrifuge, Autoclave ,Microscopy.
Glassware – Description of Glassware, its use, handling and care.
UNIT-III
Basic Steps for Specimen collection-types of specimens- Requirement of Collection -
methods of Collection of biological fluids - Anticoagulants - Preservation of samples -
Chemical preservatives . An overview on the transportation of the samples.
UNIT-IV
Process of analysing the specimens- labelling the samples. Maintaining the sample record.
Colour code for the samples. Reporting results: a. Basic format of a test report, b. Release of
examination results c. Alteration in reports Quality Management system : Internal and
External quality control. Accuracy, Precision, and Reference value. Levi-Jenning’s chart (LJ).
UNIT-V
Biomedical waste management in a clinical laboratory: Disposal of used samples, reagents
and other biomedical waste - colour coding as per guidelines.
REFERENCES:
TEXT BOOKS
1. Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl
and Edward R. Ashwood, David E. Burns fourth edition, W.B. Saunders Company.
47 Director, SBS
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Page No.38
2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
3. Textbook of MLT, P.B.Godkar. 2nd edition.2003.Bhalani publication.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS
Publishers and Distributors.
2. Medical laboratory technology Vol.1 Kanai L. Mukherjee, 2007,.Tata McGraw
Hill
PEDAGOGY: Chalk and Talk, OHP and PPT.
48 Director, SBS
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Page No.39
SEMESTER – II
MODULAR COURSE-MODULE 1
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Paper II: Techniques in Clinical Laboratory Technology
NUMBER OF HOURS/WEEK: 30 Hrs
COURSE OBJECTIVE
To gain the knowledge on the clinical assessment of various diseased conditions and routine
test followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: To understand the procedures of clinical laboratory (K2)
CO2: To understand the routine analysis of clinical samples (K2)
CO3: To understand the importance of biological samples (K2)
CO4: To understand the histopathogical procedures (K2)
CO5: To understand the blood banking procedures (K2)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 10 ESE – 40
49 Director, SBS
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CONTENTS
UNIT-I
Basic Hematological diagnosis and Clinical Chemistry: Preparation of Blood Smears-
method (Coverslip, spreader slide and Wedge), Types and causes of poor blood smears (in
diseased condition), Staining Techniques- Leishman’s stain, Wright’s stain, Field’s stain and
Romanowsky stains, Criteria for a good stain: Problem encountered during staining –
Troubleshooting. Total cell count: RBC, WBC and Platelet. Estimation of hemoglobin, PCV
& MCV, MCH, MCHC. Erythrocyte sedimentation Rate (ESR)-Westergen method and its
importance, Differential count, Determination of bleeding time, clotting time and prothrombin
time. Clinical Chemistry: Blood sugar Analysis- glucometer based analysis, Fasting,
Random, Post Prandial, HbA1C, and GTT.
UNIT-II
Collection of Urine - Biohazard management - Routine urine analysis - Colour - Clarity -
Odour - Volume - Chemical Examination - Benedict’s Test - Fehling’s test - Chemistrip
method - Test for Protein in Urine - Heat and Acetic Acid Test - Sulphosalicylic Acid Test -
Heller’s Test. Rotheral’s test, Gerhardt’s test, Fouchet’s Test – Hay’s sulphur test- Test for
Hematuria - Benzidine Test - Guaiacum Test - Gregersen’s Test. Microscopic Examination of
Urine: Crystals, casts and cells. Examination of stool- physical, chemical & microscopic
examination
UNIT-III
Body Fluids: Characteristics of Cerebrospinal Fluid, Synovial fluid, Pleural fluid, Pericardial
fluids, Peritoneal fluids and amniotic fluid. Semen analysis- physical, chemical & microscopic
examination, sperm count and motility.
UNIT-IV
Introduction to Histopathology- Basic steps for Tissue Processing- Fixing, Embedding-
methods- Microtomy- Staining- Mounting, methods of decalcifications –cryopreservation -
Equipments - Microtome -Types, Uses, Parts, different types of microtome knives, care &
maintenance. Automated tissue processor – components-Staining Methods - Hematoxylin &
Eosin stain -Mounting –visualization. Cryostat.
50 Director, SBS
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UNIT-V
Blood banking: Blood Group (ABO & Rh) – methods of grouping & reverse grouping. Basic
blood banking procedures – collection of blood, anticoagulants used, cross matching, different
screening tests including Coomb’s Test (direct and indirect), preparation of different blood
components for use and how to serve a requisition. Preparation of red cell suspension. Blood
transfusion hazards - hemolytic disease of newborn. Apheresis.
TEXT BOOKS:
1. Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl and
Edward R. Ashwood, David E. Burns fourth edition, W.B. Saunders Company.
2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
3. Handbook of Histopathological and Histochemical technique -C.F.A.Culling.3rd
edition-Butterworths, London.
4. Textbook of MLT, P.B.Godkar. 2nd edition.2003. Bhalani publication.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical Laboratory Technology Vol.1 Kanai L. Mukherjee, 2007,.Tata McGraw
Hill
PEDAGOGY: Chalk and Talk, OHP and PPT.
51 Director, SBS
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SEMESTER II
MODULAR COURSE - MODULE II
COURSE: M.Sc., BIOCHEMISTRY
SUBJECT TITLE: PAPER I: HUMAN PHYSIOLOGY
NUMBER OF HOURS/WEEK: 30 HRS
COURSE OBJECTIVE: To provide the students with in-depth knowledge on the functions
of major organs of human body.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL: CO1: To Understand the structure and functions of cells (K2).
CO2: To understand the mystery of complicated function aspects of individual organs in the
body (K2).
CO3: To understand the principles of human physiology (K2).
CO4: To distinguish the cells involved in each system (K4).
CO5: To exemplify the knowledge on the regulation of body functions (K2).
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CONTENTS UNIT I Human Anatomy and Functions- Cell and their functions – Types of muscles and mechanism of muscle contraction – Immune system – Blood components. UNIT II Anatomy and Physiology of Cardiovascular system - Anatomy and Physiology of central nervous system - Anatomy and Physiology of respiratory system. UNIT III Anatomy and physiology of Digestive system, Anatomy and Physiology of excretory system.
CIA – 10 ESE – 40
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UNIT IV Anatomy and Physiology of sensory organs, Anatomy and Physiology of endocrine system. UNIT V Anatomy and Physiology of reproductive system – Menstruation – pregnancy. REFERENCES:
1. Human Physiology - Chaterjee C C , Vol I & II, 11th edition.
2. Review of Medical Physiology - Ganong, , 24th Edition.
3. The Anatomical Basis of Clinical Practice- Gray's Anatomy, 40th Edition.
4. Textbook of Human Anatomy - Ranganathan T S, 5th edition.
5. Text book of Medical Physiology – Sembulingam and PremaSembulingam– 6th
Edition, Jaypee Brothers Medical Publishers (P) Ltd.
53 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
II Year B.Sc., MICROBIOLOGY
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS) (2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
54 Director, SBS
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REGULATIONS
INTRODUCTION
Microbiology, one of three basic fields in the biological sciences, is an extremely
diverse and complex field. It is essential to the fabric of medicine, the allied health sciences,
agriculture, ocean sciences and the vital growing biotechnology industry (genetics, cell and
molecular biology, etc.) of the present era. Our primary objectives are: 1) to produce highly
qualified and competent majors in the selected Microbiology areas, 2) to develop and
maintain a strong and supportive research program to complement our teaching and 3) to be a
center of excellence and information resource in Microbiology.
MICROBIOLOGY
Microbiology, one of three basic fields in the biological sciences, is an extremely
diverse and complex field. It is essential to the fabric of medicine, the allied health sciences,
agriculture, ocean sciences, and the vital growing biological industry (genetics, cell and
molecular biology, etc.) of the present era.
OBJECTIVES
On successful completion of the course, the students will thoroughly understand the
basic techniques used in Microbiology, various Microbiological processes, applications of
Microbiology in various fields, such as Medical Microbiology, Pharmaceutical Microbiology,
Industrial Microbiology, Immunology, Molecular Biology, Genetic Engineering,
Environmental Microbiology, Genomics & Proteomics, Virology, Food and Dairy
Microbiology and Soil and Agricultural Microbiology. Students will also focus on IPR,
Bioethics and Biosafety, HACCP and GMP.
ELIGIBILITY
A pass in Higher Secondary Examination with one of the subjects as Biology/Botany/Zoology
(Academic).
DURATION OF UG COURSE
The course shall extend over a period of three years comprising of six semesters, with
two semesters per year. There shall not be less than ninety instructional days (six periods – 50
minutes duration each /day) during each semester. Examination shall be conducted at the end of
55 Director, SBS
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Page No.3
each semester for the theory subjects and practical examinations will be conducted at the end
of the even semester.
DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT No of
Papers
Marks
@ Credits
I Language – I Tamil /Malayalam/Hindi/French 4 400 16
II Language – II English 4 400 16
III
Core Subjects 16 $ 1500 60
Allied Subjects 6** 400 16
Elective Subjects 3 300 12
Practicum - 50 2
IV
1 - Non-Major Elective – I
Basic Tamil₤/Womens Rights
2- Non-Major Elective – II
Advanced Tamil₤/General Awareness
2 100 # 4
2 – Skill Based Subjects 4 200 8
3 – Foundation Course I 1 50 # 2
4 – Foundation Course II 1 50 # 2
V Extension Activities - 50!! 2
VI Library - - -
Total 3500 140
56 Director, SBS
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Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects
respectively.
$ In core subjects both theory and practicals should be included wherever applicable.
** In allied subjects both theory and practicals should be included wherever applicable.
# No Continuous Internal assessment for these subjects
!! The Evaluation of extension activities will be based on NSS/ NCC/ SPORTS/ Red Cross
₤ Continuous Internal assessment for these subjects
The following parameters are considered throughout study period.
i) Regularity of Attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other Social Components (Blood Camp, Fine Arts etc)
Note: II
The Credit points and Lecture Hours are not linked.
57 Director, SBS
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Page No.5
Annexure No. UMB11
BOS.DT: 12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049
SCHOOL OF BIOLOGICAL SCIENCES
B.Sc., MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
Sem
este
r
Par
t
Su
b C
ode
Subject Title
Inst
. H
rs
per
wee
k
Examination Details
Cre
dit
s
Du
rati
on i
n
Hou
rs.
CIA
En
d S
emes
ter
Exa
m
Tot
al M
ark
s
SEMESTER I
I
I Language – I 6 3 25 75 100 4
II English – I 6 3 25 75 100 4
III Core Paper I – Cell Biology 4 3 25 75 100 4
III Core Paper II – Biochemistry 4 3 25 75 100 4
III Core Practical I – Cell Biology,
Biochemistry and Microbiology 2 - - - - -
III Allied Paper I – Chemistry-I-
Inorganic & Physical Chemistry 4 3 20 55 75 3
III Allied Practical I- Chemistry 2 - - - - -
IV Foundation Course I-
Environmental Studies 2 3 - 50 50 2
Total 30 - - - 525 21
SEMESTER II
II
I Language – II 6 3 25 75 100 4
II English – II 6 3 25 75 100 4
58 Director, SBS
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II
III Core Paper III – Introductory
Microbiology 5 3 25 75 100 4
III Core Practical I: Cell Biology,
Biochemistry and Microbiology 4 3 40 60 100 4
III Allied Paper II – Chemistry II –
Organic Chemistry 4 3 20 55 75 3
III Allied Practical I – Chemistry 3 3 20 30 50 2
IV Foundation Course II – Cultural
Heritage of India 2 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER III
III
I Language III 6 3 25 75 100 4
II English III 6 3 25 75 100 4
III Core Paper IV – Microbial
Taxonomy and Diversity 4 3 25 75 100 4
III Core paper V – Microbial
Physiology 4 3 25 75 100 4
III
Core Practical II – Molecular
Genetics and Microbial
Physiology
2 - - - - -
III Allied paper III – Basic
Mathematics 3 3 20 55 75 3
IV
Skill based Subject 1 –
Analytical Biochemistry and
Biomedical Instrumentation
3 3 10 40 50 2
IV Non-major elective – I
Basic Tamil / Women’s Rights 2 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER IV
IV I Language IV 6 3 25 75 100 4
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II English IV 6 3 25 75 100 4
III Core VI – Molecular Genetics 4 3 25 75 100 4
III
Core Practical II: Molecular
Genetics & Microbial
Physiology
4 6 40 60 100 4
III
Allied Paper IV: Fundamentals
of Computers & C
Programming
3 3 20 55 75 3
III
Allied Practical II:
Fundamentals of Computers &
C Programming
2 3 20 30 50 2
IV SBS 2: Bioinformatics 3 3 10 40 50 2
IV Non-major elective II - General
Awareness 2 3 - 50 50 2
Total 30 - - - 625 25
SEMESTER V
V
III Core Paper VII – Medical
Microbiology-I 4 3 25 75 100 4
III Core Paper VIII – Genetic
Engineering 5 3 25 75 100 4
III Core Paper IX – Virology 5 3 25 75 100 4
III Core Paper X – Immunology 4 3 25 75 100 4
III Elective – I 4 3 25 75 100 4
IV Skill based Subject 3: Clinical
Laboratory Technology I 3 3 10 40 50 2
III
Core Practical III – Medical,
Industrial and Agricultural
Microbiology
5 - - - - -
III Practicum - - 20 30 50 2
Total 30 - - - 600 24
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SEMESTER VI
VI
III Core Paper XI – Medical
Microbiology-II 5 3 25 75 100 4
III Core Paper XII – Industrial
Microbiology 5 3 25 75 100 4
III
Core Practical III – Medical,
Industrial and Agricultural
Microbiology
6 9 40 60 100 4
III Elective – II 5 3 25 75 100 4
III Elective–III 5 3 25 75 100 4
IV Skill based Subject 4: Clinical
Laboratory Technology II 3 3 10 40 50 2
V Extension Activities - - 50 - 50 2
VI Library 1 - - - - -
Total 30 - - - 600 24
Grand total - - - - 3500 140
61 Director, SBS
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Practicum:
Each student has to undergo an Industrial / Institutional training during the summer vacation prior to
Semester V. This should be of a minimum duration of 15 working days at any off-campus site
approved by the Director. Students are required to submit three copies of Practicum report with
attendance certificate upon completion and the bonafide report carried out by the candidate under the
guidance of a faculty authenticated and countersigned by the Director. This practicum must be
presented by the candidate in the department (at the end of fifth semester) attended by all faculty and
reviewed by the respective guide as examiner.
@ Practicum:
Attendance and internal assessment by the guide = 20 marks (Internal)
Final Viva Voce = 30 marks (External) (Dissertation = 20 marks + Viva = 10 marks)
Total Credits: 140 [Part I -16, Part II -16, Part III-90, Part IV -16, Part V-2]
Each paper carries an internal component; there is a pass minimum for External component
and overall.
Distribution of marks between Internal* and External assessment
Theory: Internal assessment (25)*: External assessment (75)
Practical: Internal assessment (40)*: External assessment (60)
COMPONENTS OF INTERNAL ASSESSMENT
Theory (for 25 marks): One Internal test and One Model test in each semester
Internal Test: 05 marks*
End semester Model test: 10 marks
One Assignment: 05 marks
Overall performance: 05 marks
Core Practicals I, II:
Min. 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Core Practical III:
Min. 10 experiments to be conducted/ semester: 20 marks
62 Director, SBS
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Average of two tests: 15 marks
Record work: 05 marks
Allied/ SBS (for 20 marks): One Internal test and One Model test in each semester
Internal Test: 04 marks*
End semester Model test: 08 marks
One Assignment: 04 marks
Overall performance: 04marks
*Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge and subject in charge. The final decision to be made by
the Director.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I Food and Dairy
Microbiology Parasitology Biomolecules
Paper II
Environmental and
Agricultural
Microbiology
Medical Biochemistry Plant Biotechnology
Paper III Pharmaceutical
Microbiology
Entrepreneurial
Microbiology Bio-nanotechnology
63 Director, SBS
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Page No.11
Annex EDC: UG MB
BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE & COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049.
B. Sc., MICROBIOLOGY
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2014 onwards)
Note:
The student has to compulsorily select one Extra Disciplinary Course offered by other
departments and is eligible to get 2 extra credits. This paper is offered as self-study from the
2014 batch onwards.
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m i
n
hrs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Semester V
VI Mushroom cultivation - 3 10 40 50 2
Total - - - - 50 2
64 Director, SBS
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SEMESTER – III
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper IV: Microbial Taxonomy and Diversity
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
This paper presents the study of nomenclature and importance of taxonomy for
microorganisms based on the Bergey’s manual. The paper also gives an insight to general
characters, morphology and economic importance to algae and fungi.
GOAL
This paper in Microbiology has been designed to provide the student a firm foundation in the
taxonomy, which forms a base for their future study.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of
taxonomy of microorganisms.
CONTENTS
UNIT-I
Introduction to evolution-Endosymbiotic theory.Binomial nomenclature of microbes - species
concept- description of organisms- classical approach with examples. Classical method and
numerical method of classification. Classification of microorganisms - Haeckel and Woose
three kingdom concept, Whittaker’s five kingdom concept,. Modern trends in classification-
Ribotyping-16s rRNA, Nucleic acid hybridization.
UNIT-II
Salient features of second edition of Bergey’s manual (Volume I and II) – Outline
characteristics of Archae bacteria and eubacteria. Introduction to archaebacteria -archaeal cell
wall, archaeal lipids and membranes. Introduction to phylum Euryarchaeota and
crenarchaeota.
CIA – 25
ESE – 75
65 Director, SBS
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UNIT-III
Fungi: Outline classification up to genus level, main classes of fungi: Ascomycetes
(Saccharomyces), Basidiomycetes (Agaricus), Zygomycetes (Rhizopus), Oomyctes
(Allomyces) and Deuteromycetes (Aspergillus) - general characteristics, reproduction and its
economic importance.
UNIT-IV
Algae: Classification, general characteristics and reproduction and its economic importance –
Chlorophyta, Euglenophyta, Chrysophyta, Phaeophyta, Rhodophyta, Bacillariophya and
cyanobacteria.
UNIT-V
Protozoa: Classification, general characteristics and reproduction of phylum
Sarcomastigophora, Labyrinthomorpha, Apicomplexa, Microspora, Myxozoa and Ciliophora.
TEXT BOOKS:
1. Microbiology: Concepts and Applications. Michael J. Pelczar Jr. (1993) McGraw-
Hill. Inc.
REFERENCE BOOKS:
1. Bergey’s Manual of Systematic Bacteriology. Krieg, N.R. and J.G. Holt (1986) ed.
2. Microbiology: Fundamentals and Applications. Ronald M. Atlas (1989) 2nd ed.
Maxwell Macmillan International Editions.
3. Microbiology. Prescott, L.M, Harley, JP. and Klein, DA. (1993) 2nd ed. WMC Brown
Publishers.
66 Director, SBS
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Page No.14
SEMESTER – III
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper V: Microbial Physiology
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
This paper presents the study of microbial nutrition and growth patterns. The information
about microbial metabolism and fermentative pathways help the students to understand about
microbial processing of substrates. Concepts of microbial photosynthesis in aerobic and
anaerobic conditions are also studied.
GOAL
This paper in microbiology has been designed to provide the student with a firm foundation in
the physiological aspects of microorganisms, which forms a base for their future research.
This paper also provides the basics in metabolism aspects for better understanding of
microbial physiology.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of
microbial classification based on mode of nutrients, growing patterns of microbes, metabolic
cycles and fermentative pathways used by microbes to metabolize the nutrients and microbial
photosynthesis.
CONTENTS
UNIT- I
Nutrition- Nutritional requirements of microorganisms – classification based on nutritional
modes of organisms - Autotrophs, Heterotrophs, Phototrophs, Chemoautotrophs, Copiotrophs,
Oligotrophs. Nutritional classification based on carbon and electron sources. Transport of
nutrients- active passive and facilitated diffusion – carrier mediated and Iron transport.
CIA – 25
ESE – 75
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UNIT-II
Definition of growth – balanced and unbalanced growth, Different phases of growth – Growth
curve – generation time – factors influencing microbial growth – Intrinsic and Extrinsic
factors temperature, pH, oxygen, Pressure, Salt concentration, Moisture, Radiation. Bacterial
growth kinetics - batch, continuous and synchronous culture. Diauxic growth curve.
UNIT-III
Carbohydrate metabolism – Glycolysis, EMP pathway, HMP shunt, ED pathway – TCA
cycle. Aerobic respiration – Oxidative phosphorylation, ETC, ATP generation. Anaerobic
respiration – Sulfur, nitrate and carbon dioxide as electron acceptor.
UNIT-IV
Fermentative pathways– Alcohol and organic acid (Lactic acid, Propionic acid and mixed acid
fermentation), Methane fermentation.
UNIT-V
Microbial Photosynthesis- oxygenic (Cyanobacteria) and anoxygenic (Green sulfur and purple
non sulfur bacteria). Carbon dioxide fixation –C3, C4 pathways, Bioluminescence.
TEXT BOOKS:
1. Microbiology. Prescott, LM, Harley, JP. and Klein, DA. (1993) 2nd ed. WMC Brown
Publishers.
2. General Microbiology. Roger Y. Stanier, (1987) 5th Ed. McMillan Press, Cornell
University.
REFERENCE BOOKS:
1. Microbial Physiology. Albert G. Moat and John W. Foster (1988) 4th ed. John Wiley
and Sons.
2. Microbial physiology and Metabolism. Caldwell, DR. (1995) Brown publishers
3. Chemical Microbiology: An introduction to Microbial physiology. Rose. A.H.
(1986) Wiley and Sons.
4. Bacterial Metabolism. Gottschalk, G. (1986) 2nd ed. NY: Springer –Verlag.
5. Bacterial Metabolism. Doelle, HW. (2005) 2nd ed. Elsevier publications.
68 Director, SBS
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SEMESTER – III
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Practical II: Molecular Genetics and Microbial Physiology
NUMBER OF HOURS/WEEK: 2 Hrs
SUBJECT DESCRIPTION
This practical paper makes the students to get hands on training to handle the microbes safely,
analyze them and identify. By providing basic instrumentation knowledge, the students can
analyze the microbial genetic material using analytical methods.
GOAL
This practical paper in microbiology has been designed to provide the student with a firm
foundation in the aspects of analyzing; culturing and describing the physiological
characteristics features of the microbes isolated and based on the acquired result get
identified.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of
handling the microbes in laboratory like culturing, analyzing and identifying them.
Understanding DNA and plasmids of microbial origin and its analysis.
CONTENTS
MOLECULAR GENETICS:
1. Isolation of bacterial DNA and its separation using agarose gel electrophoretic
technique (Demonstration).
2. Isolation of bacterial plasmid and its separation using agarose gel electrophoretic
technique (Demonstration).
3. Transformation (demonstration).
4. Auxotrophic mutants – UV and Chemical (EMS)
CIA – 40
ESE – 60
69 Director, SBS
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MICROBIAL PHYSIOLOGY:
5. Measurement of microbial growth –– Turbidity methods – determination of
generation time.
6. Cultivation of anaerobic organisms – Wright’s tube method – McIntosh jar.
7. Isolation of Thermophiles.
8. Isolation of Halophiles.
9. Biochemical characteristics of microorganisms:
a. Indole production test
b. Methyl red test
c. Voges Proskauer test
d. Citrate utilization test
e. Carbohydrate fermentation
f. Hydrogen sulfide production test
g. Triple sugar iron test
h. Oxidase test
i. Catalase test
j. Urease test
k. Nitrate reduction test
10. Polymer degradation –Starch, Casein and Gelatin
REFERENCES
1. Experiments in microbiology plant pathology and biotechnology – Aneja, KR.
(2003) 4th Ed. New age international publ.
2. Microbiology – Laboratory Manual - James G. Cappuccino and Natalie Sherman
(1999) 6th ed. Benjamin/Cummings Science Publishing.
3. Microbiology Manual – Kannan, N, (2000) Palani Paramount Publishing.
4. Microbiology-Laboratory Manual – Sudarajan, M. (2001).
5. Experimental procedures in Lifesciences - Rajan, S. (2010) Anjana book house.
6. Molecular Cloning: A Laboratory Manual – Michael R. Green and Joseph
Sambrook (2001) 4th ed. Vol. II. Cold Spring Harbour Laboratory Press.
70 Director, SBS
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SEMESTER – III
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Skill Based Subject I – Analytical Biochemistry and Biomedical
Instrumentation
NUMBER OF HOURS/WEEK: 3 Hrs
SUBJECT DESCRIPTION
This subject presents the principles, instrumentation, working and application of the
instruments commonly used in the laboratories.
GOALS
To enable the students to learn about the functioning, components and applications of various
commonly used instruments.
OBJECTIVES
By successfully completing this subject a student will:
Become aware of strategies and methods for biochemical analysis as well as the
instrumentation.
Understand the working principles of analytical instruments and underlying chemistry of
methods so that their applicability, advantages, limitations and sources of error.
Learn to interpret and critically evaluate analytical data and to report and discuss the
results of biochemical analyses.
CONTENTS
UNIT-I
Chromatography - Principle, materials, methods and applications of paper chromatography,
TLC, GLC, Adsorption, Ion-exchange, Affinity and Molecular sieve chromatography.
Reversed phase, HPLC (principle and applications only).
CIA – 10
ESE – 40
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Page No.19
UNIT-II
Electrophoresis - Principle, instrumentation and applications of paper electrophoresis, agar
gel, starch gel, SDS-PAGE, immunoelectrophoresis and isoelectric focusing.2D Gel
Electrophoresis, (Overview only).
UNIT-III
Centrifugation –principle and types, bench top, high speed and ultra centrifuge. Principle and
applications of Analytical Centrifuge. Determination of Molecular weight by Sedimentation
velocity method. Separation of Subcellular Organelles.
UNIT-IV
Electromagnetic spectrum & laws of absorption. Principle, Instrumentation and applications
of Colorimetry, Spectrophotometry, Fluorimetry and Flame photometry.
Principle and applications of Atomic Absorption, Circular Dichroism, X-ray diffraction and
NMR Spectroscopy.
ECG, EEG, CT scan, Doppler, MRI scan (Principle and applications only). Blood gas and
electrolyte analysers.
UNIT-V
Radioactivity: Isotopes, Radioactivity, Radioactive decay, Interaction of radioactivity with
matter-Excitation and Ionisation. Measurement of Radioactivity: Methods based on
ionization-Ionization counter, Proportional counter, Geiger counter. Methods based on
excitation-Scintillation counting. Application of radioisotopes in biology and medicine.
Autoradiography.
TEXTBOOKS:
1. Biophysical Chemistry – Principles and Techniques - Upadhyay & Upadhyay, 4th
Edition, (2016) Himalayan Publications House.
2. Practical Biochemistry Principles & techniques Edited by Keith Wilson & John Walker
5th Edition Cambridge University Press.
72 Director, SBS
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Page No.20
REFERENCE BOOKS:
1. Instrumental method of chemical analysis, B K Sharma, (2014) Krishna Prakashan
Media P Ltd.
2. An Introduction to Practical Biochemistry, David T. Plummer, 3rd Edition (2001)
McGraw Hill Education.
3. A Biologists guide to Principles and Techniques of practical Biochemistry- Keith
Wilson, Kenneth H. Goulding, 3rd Edition 1992. Cambridge University Press.
4. Biomedical Instrumentation and Measurement, R Ananda Natarajan, 2nd Edition
(1995) Prentice – Hall of India Pvt Ltd
73 Director, SBS
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Page No.21
SEMESTER – IV
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper VI – Molecular Genetics.
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
Molecular genetics is concerned with the development of biochemical and genetic techniques
for handling the complex nucleic acids that constitute genetic material.
GOALS
To make the students to understand the concept of gene, chromosomes and gene transfer
mechanism.
OBJECTIVES
On successful completion of the course the students understand the concepts of Genetics and
its importance.
CONTENTS
UNIT-I
Mendel’s experiments and principle of segregation, monohybrid cross- Dominance,
Recessiveness, Codominance and Lethals; Principles of independent assortment- Dihybrid
cross, multiple alleles- ABO Blood type and Rh Factor alleles, Gene Interaction – Epistasis
and Non – Epistasis. Chromosomal theory of inheritance.
UNIT-II
Structure and function of DNA and RNA. DNA replication – conservative, semiconservative
and dispersive. Types of replication- (Rolling circle and θ mode). Enzymes involved in the
replication of DNA.
UNIT-III
Mechanism of Transcription: General principles - types of RNA polymerases, processing
steps. Initiation, elongation and termination. Post – transcriptional modifications in RNA:
CIA – 25
ESE – 75
74 Director, SBS
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(capping, polyadenylation and splicing). Basic features of the genetic code. Protein synthesis:
initiation, elongation and termination. Operon concept – lac operon and Tryptophan operon.
UNIT-IV
Transformation: Discovery, Mechanism and Genetic mapping of Transformation.
Conjugation: Discovery, F+, F- and Hfr cells, Mechanism and Genetic mapping of
Conjugation. Transduction: Discovery, Mechanism (generalized and specialized) and Genetic
mapping of Transduction.
UNIT- V
Mutation - Spontaneous and induced - types of mutations - mutagens (Physical and chemical)
and mutagenesis. DNA repair mechanisms - Mismatch, base excision and SOS repair.
Transposons: Introduction - Discovery insertion sequences, complex and compound
transposons- Mechanism of transposition (Tn3).
TEXT BOOKS:
1. Microbial Genetics - David freifielder. (2002). Narosa publishing house, New Delhi.
2. Genetics - Ahluwalia K.B, (1996). New age international Pvt. Ltd.
REFERENCE BOOKS:
1. Principles of Genetics - Elson John Gardner, Michael J. Simmons & D. Peter Snustad
(2005) 8th ed. John Wiley & sons (Asia) Pvt. Ltd.,
2. Genes VIII - Benjamin Lewin (2004) Pearson prentice Hall, USA.
3. Essential of Molecular Biology - Brown TA. (2003) Freeman publishing House.
4. Genetics - Peter J. Russel (2002) Benjamin Cummings.
5. Principles of Genetics - Robert H. Tamarin (2004) 7th ed. Tata McGraw publishing
house, New Delhi.
75 Director, SBS
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SEMESTER – IV
COURSE: B.Sc., MICROBIOLOGY
SUBJECT TITLE: CORE PRACTICAL II: Molecular Genetics and Microbial
Physiology
NUMBER OF HOURS/WEEK: 4 Hrs
Refer semester III for Core Practical II syllabus
CIA – 40
ESE – 60
76 Director, SBS
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Page No.24
SEMESTER – IV
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Skill Based Subject II – Bioinformatics
NUMBER OF HOURS/WEEK: 3 Hours
SUBJECT DESCRIPTION
This subject emphasizes on various tools in bioinformatics and methods to determine the
alignment between the sequences.
GOALS
To enable the students to learn the basic computational skills in bioinformatics-sequence
analysis.
OBJECTIVES
On successful completion of the subject the students should have understood comparison of
sequences to find similar sequences, comparison of homologous sequences to construct a
molecular phylogeny and Visualization of 3D structures of protein.
CONTENTS
UNIT -I
Introduction to bioinformatics and data generation: Bioinformatics and its relation with
molecular biology. Scope of Bioinformatics, Tools in Bioinformatics. Applications of
Bioinformatics.
UNIT -II
Biological Database: Types - Nucleic acid databases (NCBI, DDBJ, and EMBL). Protein
databases (Primary, Composite, and Secondary). Carbohydrate databases. Specialized
Genome databases: (SGD and TIGR). Structure databases (CATH, SCOP, and PDB). File
Format- (Genbank, DDBJ and SwissProt).
CIA – 10
ESE – 40
77 Director, SBS
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UNIT -III
Sequence Alignments and Visualization: Introduction to Sequences - Introduction to single
letter code of aminoacids, Symbols used in nucleotides, Alignments, Scoring matrices- PAM
and BLOSSUM, Dot plot and Dynamic Programming: Local alignment and Global alignment
(algorithm and example). Pairwise sequence alignment– BLAST and FASTA. Multiple
sequence alignment- CLUSTALW.
UNIT -IV
Phylogenetic Analysis - Introduction, Phylogenetic tree, rooted and unrooted tree, Method for
determining phylogenetic tree, Distance based method, Character based method.
UNIT -V
Visualisation tools- Rasmol, Swisspdb viewer, Jmol, webmol, chime. Conserved sequences-
Motif and Domain.
TEXT BOOKS :
1. Bioinformatics methods and applications Rastogi. S. C et al., 1993. Prentice Hall of
India Pvt Ltd, New Delhi,4th ed., India.
REFERENCE BOOKS :
1. Bioinformatics Sequence and Genome Analysis. David W. Mount, 2nd Edition
(2004).
2. Trends in Bioinformatics. 2006. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
3. Principles of Bioinformatics. 2005. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
4. Introduction to Bioinformatics. Attwood A.T and D.J Parry-smith, Pearson
education ltd.
78 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
III Year B.Sc., MICROBIOLOGY
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS)
(2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2015)
79 Director, SBS
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REGULATIONS
INTRODUCTION
Microbiology, one of three basic fields in the biological sciences, is an extremely
diverse and complex field. It is essential to the fabric of medicine, the allied health sciences,
agriculture, ocean sciences and the vital growing biotechnology industry (genetics, cell and
molecular biology, etc.) of the present era. Our primary objectives are: 1) to produce highly
qualified and competent majors in the selected Microbiology areas, 2) to develop and
maintain a strong and supportive research program to complement our teaching and 3) to be a
center of excellence and information resource in Microbiology.
MICROBIOLOGY
Microbiology, one of three basic fields in the biological sciences, is an extremely
diverse and complex field. It is essential to the fabric of medicine, the allied health sciences,
agriculture, ocean sciences and the vital growing biological industry (Genetics, cell and
Molecular biology, etc.,) of the present era.
OBJECTIVES
On successful completion of the course, the students will thoroughly understand the
basic techniques used in Microbiology, various Microbiological processes, applications of
Microbiology in various fields, such as Medical Microbiology, Pharmaceutical Microbiology,
Industrial Microbiology, Immunology, Molecular Biology, Genetic Engineering,
Environmental Microbiology, Genomics & Proteomics, Virology, Food and Dairy
Microbiology and Soil and Agricultural Microbiology. Students will also focus on IPR,
Bioethics and Biosafety, HACCP and GMP.
ELIGIBILITY
A pass in Higher Secondary Examination with one of the subjects as Biology/Botany/Zoology
(Academic).
DURATION OF UG COURSE
The course shall extend over a period of three years comprising of six semesters, with
two semesters per year. There shall not be less than ninety instructional days (six periods – 50
minutes duration each /day) during each semester. Examination shall be conducted at the end
of each semester for the theory subjects and practical examinations will be conducted at the
end of the even semester.
80 Director, SBS
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DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT No. of
Papers
Marks
@ Credits
I Language – I Tamil /Malayalam/Hindi/French 4 400 16
II Language – II English 4 400 16
III
Core Subjects 16 $ 1500 60
Allied Subjects 6** 400 16
Elective Subjects 3 300 12
Practicum - 50 2
IV
1 - Non-Major Elective – I
Tamil₤/Advanced Tamil₤/Communicative
English
2- Non-Major Elective – II
Tamil₤/ Advanced Tamil₤/ General Awareness
2 100 # 4
2 – Skill Based Subjects 4 200 8
3 – Foundation Course I 1 50 # 2
4 – Foundation Course II 1 50 # 2
V Extension Activities - 50!! 2
VI Library - - -
Total 3500 140
Note: I
@Includes 25/40% continuous assessment marks for theory and practical subjects
respectively.
$ In core subjects both theory and practical’s should be included wherever applicable.
** In allied subjects both theory and practical’s should be included wherever applicable.
#No Continuous Internal assessment for these subjects
81 Director, SBS
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Page No.4
!! The Evaluation of extension activities will be based on NSS/ NCC/ SPORTS/ Red Cross
₤Continuous Internal assessment for these subjects
The following parameters are considered throughout study period.
i) Regularity of Attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other Social Components (Blood Camp, Fine Arts etc)
Note: II
The Credit points and Lecture Hours are not linked.
82 Director, SBS
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Page No.5
Annexure No. UMB11
BOS.DT: 12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049
SCHOOL OF BIOLOGICAL SCIENCES
B.Sc., MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2015)
Sem
este
r
Par
t
Su
b C
ode
Subject Title
Inst
. H
rs
per
wee
k
Examination Details
Cre
dit
s
Du
rati
on i
n
Hou
rs
CIA
En
d S
emes
ter
Exa
m
Tot
al M
ark
s
SEMESTER I
I
I Language – I 6 3 25 75 100 4
II English – I 6 3 25 75 100 4
III Core Paper I – Cell Biology 4 3 25 75 100 4
IV Core Paper II – Biochemistry 4 3 25 75 100 4
V Core Practical I – Cell Biology, Biochemistry and Microbiology
2 - - - - -
VI Allied Paper I – Chemistry-I 4 3 20 55 75 3
VII Allied Practical I- Chemistry 2 - - - - -
VIII Foundation Course I-Environmental Studies
2 3 - 50 50 2
Total 30 - - - 525 21
SEMESTER II
II
I Language – II 6 3 25 75 100 4
II English – II 6 3 25 75 100 4
III Core Paper III – Introductory Microbiology
5 3 25 75 100 4
83 Director, SBS
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IV Core Practical I: Cell Biology, Biochemistry and Microbiology
4 3 40 60 100 4
V Allied Paper II – Chemistry- II 4 3 20 55 75 3
VI Allied Practical I – Chemistry 3 3 20 30 50 2
VII Foundation Course II – Cultural Heritage of India
2 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER III
III
I Language III 6 3 25 75 100 4
II English III 6 3 25 75 100 4
III Core Paper IV – Microbial Taxonomy and Diversity
4 3 25 75 100 4
IV Core paper V – Microbial Physiology
4 3 25 75 100 4
V Core Practical II –Molecular Genetics and Microbial Physiology
2 - - - - -
VI Allied paper III – Basic Mathematics
3 3 20 55 75 3
VII Skill based Subject 1 – Analytical Biochemistry and Biomedical Instrumentation
3 3 20 30 50 2
VIII Non-major elective – I
Basic Tamil / Women’s Rights 2 3 - 50 50 2
Total 30 - - - 575 23
SEMESTER IV
IV
I Language IV 6 3 25 75 100 4
II English IV 6 3 25 75 100 4
III Core Paper VI – Molecular Genetics
4 3 25 75 100 4
84 Director, SBS
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Page No.7
III Core Practical II: Molecular Genetics and Microbial Physiology
4 6 40 60 100 4
IV Allied Paper IV: Fundamentals of Computers & C Programming
3 3 20 55 75 3
V Allied Practical II: Fundamentals of Computers and C Programming
2 3 20 30 50 2
VI SBS 2: Bioinformatics 3 3 20 30 50 2
VII Non-major elective II - General Awareness
2 3 - 50 50 2
Total 30 - - - 625 25
SEMESTER V
V
I Core Paper VII – Medical Microbiology-I
4 3 25 75 100 4
II Core Paper VIII – Genetic Engineering
5 3 25 75 100 4
III Core Paper IX – Virology 5 3 25 75 100 4
IV Core Paper X - Immunology 4 3 25 75 100 4
V Elective – I 4 3 25 75 100 4
VI Skill based Subject 3: Clinical Laboratory Technology I
3 3 20 30 50 2
VII Core Practical III – Medical Microbiology, Industrial and Agricultural Microbiology
5 - - - - -
VIII Practicum* - - 20 30 50 2
Total 30 - - - 600 24
SEMESTER VI
VI
I Core Paper XI – Medical Microbiology-II
5 3 25 75 100 4
II Core Paper XII –Industrial Microbiology
5 3 25 75 100 4
85 Director, SBS
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Page No.8
III Core Practical III – Medical, Industrial and Agricultural Microbiology
5 9 40 60 100 4
IV Elective – II 5 3 25 75 100 4
V Elective–III 5 3 25 75 100 4
VI Skill based Subject 4: Clinical Laboratory Technology-II
3 3 20 30 50 2
VII Extension Activities - - 50 - 50 2
VI Library 1 - - - - -
Total 30 - - - 600 24
Grand total - - - - 3500 140
Practicum:
Each student has to undergo an Industrial / Institutional training during the summer vacation
prior to Semester V. This should be of a minimum duration of 15 working days at any off-
campus site approved by the Director. Students are required to submit three copies of
Practicum report with attendance certificate upon completion and the bonafide report carried
out by the candidate under the guidance of a faculty authenticated and countersigned by the
Director. This practicum must be presented by the candidate in the department (at the end of
fifth semester) attended by all faculty and reviewed by the respective guide as examiner.
@ Practicum:
Attendance and internal assessment by the guide = 20 marks (Internal)
Final Viva Voce = 30 marks (External) (Dissertation = 20 marks + Viva = 10 marks)
Total Credits: 140 [Part I -16, Part II -16, Part III-90, Part IV -16, Part V-2]
Each paper carries an internal component; there is a pass minimum for External component
and overall.
Distribution of marks between Internal* and External assessment
Theory: Internal assessment (25)*: External assessment (75)
Practical: Internal assessment (40)*: External assessment (60)
86 Director, SBS
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Page No.9
COMPONENTS OF INTERNAL ASSESSMENT
Theory (for 25 marks): One Internal test and One Model test in each semester
Internal Test: 05 marks*
End semester Model test: 10 marks
One Assignment: 05 marks
Overall performance: 05 marks
Core Practicals I, II: (for 40 marks):
Min. 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Core Practical III:
Min. 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Allied/ SBS (for 20 marks): One Internal test and One Model test in each semester
Internal Test: 04 marks*
End semester Model test: 08 marks
One Assignment: 04 marks
Overall performance: 04marks
*Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge and subject in charge. The final decision to be made by
the Director.
87 Director, SBS
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Page No.10
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I Food and Dairy
Microbiology Parasitology Biomolecules
Paper II
Environmental and
Agricultural
Microbiology
Medical Biochemistry Plant Biotechnology
Paper III Pharmaceutical
Microbiology
Entrepreneurial
Microbiology Bio-nanotechnology
88 Director, SBS
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Page No.11
Annex EDC: UG MB
BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE & COMMERCE (Autonomous)
COIMBATORE – 641 049.
B. Sc., Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2014 onwards)
Note:
The students has to compulsorily select one Extra Disciplinary Course offered by
other departments and are eligible to get 2 extra credits. This paper is offered as self-
study from the 2014 batch onwards.
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m
in h
rs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Semester V
VI Mushroom cultivation - 3 10 40 50 2
Total - - - - 50 2
89 Director, SBS
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Page No.12
SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Extra Disciplinary Course – Mushroom Cultivation
NUMBER OF HOURS/WEEK: Nil
SUBJECT DESCRIPTION
This paper presents the technology and process involved in mushroom cultivation.
GOALS
To make the student to understand the methods involved in mushroom cultivation.
OBJECTIVES
On successful completion the subject student should have understood: What is Mushroom, its
types, how to cultivate and become an entrepreneur using the technology?
CONTENTS
UNIT-I
Mushroom Technology: Introduction, History and Scope - Edible and Poisonous
Mushrooms. Vegetative characters - Formation and development of Basidiocarp, structure of
basidiocarp - Agaricus. Importance and nutritive value of edible mushrooms.
UNIT-II
Cultivation System and Farm design: Fundamentals of cultivation system- small village
unit & larger commercial unit. Principles of mushroom farm layout- location of building plot,
design of farm, bulk chamber, composting platform, equipments and facilities, pasteurization
room & growing rooms.
UNIT-III
Compost and Composting: Principles of composting, machinery required for compost
making, materials for compost preparation. Methods of Composting - Long method of
composting (LMC) and Short method of composting (SMC).
CIA – 10
ESE – 40
90 Director, SBS
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Page No.13
UNIT-IV
Spawn and Spawning: Facilities required for spawn preparation, Preparation of spawn
substrate, preparation of pure culture, media used in raising pure culture, culture maintenance,
and storage of spawn.
UNIT-V
Cultivation of Button and Oyster Mushrooms: Cultivation of button mushroom (Agaricus
bisporus), oyster mushroom (Pleurotus florida) - Collection of raw materials, compost &
composting, spawn & spawning, casing & case run, croping & crop management, picking &
packing.
TEXT BOOKS:
1. Mushroom Cultivation, Tripathi, D. P. (2005) Oxford & IBH Publishing Co. Pvt.
Ltd, New Delhi.
REFERENCE BOOKS:
1. Mushroom Production and Processing Technology, Pathak Yadav Gour (2010)
Published by Agrobios (India).
2. A hand book of edible mushroom, S. Kannaiyan & K. Ramasamy (1980). Today &
Tomorrows printers & publishers, New Delhi
3. Handbook on Mushrooms, Nita Bahl, Oxford & IBH Publishing Co.
91 Director, SBS
Anx.UMb 11,3B.Sc. MB (CMS College) 2017 2015-18 batch
Page No.14
SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper VII: Medical Microbiology – I
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
Medical microbiology is a branch of medicine concerned with the prevention, diagnosis and
treatment of infectious diseases. In addition, this field of science studies various clinical
applications of microbes for the improvement of health.
GOALS
To gain knowledge on the aspects of the interrelationship between infectious agents and their
hosts.
OBJECTIVES
On successful completion of the subject, the student will know about the mechanism of
infection and its types & provides the information about the prevention of diseases.
CONTENTS
UNIT-I
Safety precautions in Microbiology Lab- Specimen collection - Handling, Transport -
Definitions of pathogen, Saprophytes, Commensals and carrier types.
UNIT-II
Infections - sources of infections - types of infections - mode of transfer- definitions,
Epidemiology, Infectious disease cycle - Investigation and Control measures of epidemic,
pandemic, endemic.
CIA – 25
ESE – 75
92 Director, SBS
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Page No.15
UNIT-III
Morphology, pathogenicity and lab diagnosis - Gram positive organisms: Bacillus anthracis,
Corynebacterium diphtheriae, Clostridium tetani. Staphylococcus aureus and Streptococcus
pneumonia.
UNIT-IV
Morphology, pathogenicity and lab diagnosis - Gram negative organisms - Proteus vulgaris,
Shigella dysenteriae, Pseudomonas aeruginosa, E. coli, Salmonella typhi and Vibrio
cholerae.
UNIT-V
Morphology, pathogenicity and lab diagnosis - Mycobacterium leprae, M. tuberculosis,
Leptospira species, Mycoplasma pneumoniae, Yersinia pestis and Yersinia entercolitica.
TEXT BOOKS:
1. Textbook of Microbiology. Ananthanarayanan R and C K Jayaram panicker. (2005).
7th edition, Orient Longman pvt Ltd.
REFERENCE BOOKS:
1. Medical Microbiology. Jawetz E and JL Melnic. (2001). Tata McGraw publishing
house, New Delhi.
2. Medical Microbiology. Patric R Murray. (1990). Mosby Publications.
3. Principles of Bacteriology, Virology and Immunology. Topley and Wilson. (1995).
Edward Arnold, London.
93 Director, SBS
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Page No.16
SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper VIII: Genetic Engineering
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This course presents the principles of Genetic Engineering for the production of useful
biological products.
GOAL
To make the students to understand the concept of vector preparation, gene manipulation and
gene transfer technologies.
OBJECTIVE
On successful completion of the subject, students should have understood: Biology of
plasmid, usability of plasmid and viral particles as vectors and cloning strategies.
CONTENTS
UNIT-I
Introduction to Gene manipulation: Steps involved in gene cloning, Enzymes used in Gene
manipulation- Restriction endonucleases- Types and features - adaptors and linkers -
Methylases, End modifying enzymes and Ligases.
UNIT-II
Purification of DNA from living cells: Preparation of Total cell DNA from E. coli,
Preparation of Plasmid DNA, Preparation of Bacteriophage DNA- Lambda and M13.
Chemical synthesis of DNA.
UNIT-III
Vectors: Properties of a vector; Plasmids - Natural plasmid vectors - pSC101 and 2µm
Plasmid, Artificial Plasmid vectors - pBR322, pUC 8. Bacteriophage vectors -M13 phage
CIA – 25
ESE – 75
94 Director, SBS
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Page No.17
vectors and lambda phage vectors - insertional and replacement vectors. Cosmids, phagemids
and phasmids, Yeast vectors- YEP, YAC.
UNIT-IV
Gene Transfer Methods and clone identification: Transformation in E. coli - Competent cell
preparation, Selection and identification of recombinants - Recombinant selection with
pBR322 (Direct and insertional inactivation methods), Recombinant selection with pUC;
Introduction of phage DNA into bacterial cells- Transfection, in vitropackaging of λcloning
vectors, Identification of recombinant phages; Construction of cDNA library, Clone
identification- Colony and plaque hybridization.
UNIT-V
Identification of gene location and sequencing: Methods used to locate a gene on small and
large DNA; DNA sequencing- Sanger Coulson method and Maxam Gilbert method. rDNA
amplification by PCR.
TEXT BOOKS:
1. Introduction to gene cloning – Brown T.A, Stanley, (1998). 3rd Ed. Thomas Pub ltd,
Germany.
2. Principles of gene manipulation. Old, R.W. and Primrose S.B. (2003). 6th Ed. Black
Well Sci Ltd, Germany.
3. Gene Biotechnology – Jogdand, (1997), Himalaya Publishing House, Mumbai.
REFERENCE BOOKS:
1. From Genes to Clones – Ernst. L. Winnacker, (2003). 3rd Ed. Panima Publishing
Corporation, New Delhi.
2. Genes VIII, Benjamin Lewin (2004), Pearson Education Corporation, New Jersy.
3. Molecular Biology. Friefielder. D, (1987). 2nd ed. Narosa publishing house, New
Delhi.
4. Recombinant DNA Technology. J. D. Watson, (2001). 2nd Ed. WH Freeman and
Company, New York.
5. Molecular Biotechnology. Glick and Pasternak, (1996). Panima Publishing
Corporation, New Delhi.
95 Director, SBS
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Page No.18
6. Molecular Cloning: A Laboratory Manual. Sambrook, J., Fritsch, E. F and
Maniatis, T., (2000). Cold Spring Harbor Laboratory Press, New York.
96 Director, SBS
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Page No.19
SEMESTER V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core paper IX: Virology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This course presents the basics of virology which deals with the structure and properties of
viruses.
GOALS
To enable students to learn about different types of viruses, it’s structural and genetic
properties and its pathogenicity.
OBJECTIVES
On successful completion of the course the students will be aware of structural features of
different classes of viruses.
CONTENTS
UNIT-I
Early development of virology – general properties of viruses- cultivation of Viruses - virus
purification and assays. The structure of viruses- virion size - General structure properties-
helical capsids, icosahedral capsid - nucleic acids - Viral envelopes and enzymes - virus
classification.
UNIT-II
Reproduction of DNA phages- double stranded DNA lytic phages- lytic cycle of T4 phage.
The one step growth- adsorption to the host cell and penetration - synthesis of Phage nucleic
acids and protein assembly of phage particles- release of phage particles. Example of single
stranded DNA phage- фX 174 –Rolling circle replication.
UNIT-III
CIA – 25
ESE – 75
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Lysogeny- temperate bacteriophages - lambda phage - induction of lysogens - Generation of
defective phages and their uses. Example of RNA phage and its reproduction.
UNIT-IV
Viruses of Eukaryotes - General Classification of plant viruses –Structure, Reproduction and
life cycle and pathogenicity of plant viruses –Tobacco Mosaic Virus, Cauliflower Mosaic
Virus,Potato virusX
UNIT-V
Viruses of Eukaryotes General Classification of animal viruses – Structure, Reproduction and
life cycle and pathogenicity and diagnosis of Hepatitis (A.B). Mumps, AIDS, Rabies,
Influenza, Measles, Rubella, Herpes simplex I & II. Oncoviruses-DNA Virus-Epstein Barr
Virus and Human Papilloma Virus, RNA virus-Hepatitis C Virus and Human T lymphocytic
virus.
TEXT BOOKS:
1. Introduction to Viruses - Amita Biswas, 2008. Vikas Publishing House (P) Ltd. New
Delhi.
REFERENCE BOOKS:
1. General Virology. Luria S.E. Darnel, J.E Jr. Baltimore. D and Campbell A. 1978, 3rd
edition, Wiley and sons.
2. Microbiology. Prescott L.M, Harley, J.P Klein D.A. 1900.WMC Publishers.
3. Introduction to modern virology. Dimmok and Primrose S.B.1994, 4th edition,
Blackwell scientific company publications.
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SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core paper X – Immunology
NUMBER OF HOURS/WEEK: 4Hrs
SUBJECT DESCRIPTION
This subject describes the defense mechanisms of the higher vertebrates against invading
pathogens, tumor immunology and autoimmunity.
GOALS: To understand the basics of human defense mechanisms against the invading
foreign bodies.
OBJECTIVES
On successful completion of the subject, the student will be aware of what immunity is, how
the immune system discriminates self from non-self antigens, how it is regulated, as well as
basic immunological techniques and their applications.
CONTENTS
UNIT-I
History and Scope of Immunology - Cell and Organs involved in immune system- T cells (TH
cells, TC cells, TS cells), B cells and NK cells, Monocytes and Macrophages, Dendritic cells,
Neutrophils, Eosinophils, Basophils and Mast cells, Organization and structure of lymphoid
organs- Primary lymphoid organs- Bone marrow, Thymus- Secondary lymphoid tissues -
spleen, lymph nodes, gut associated lymphoid tissue.
UNIT-II
Antigen - types- characteristics - Immunoglobulins – general structure, types and biological
functions. Complement pathways - classical and alternate. Immunity – Innate and acquired
immunity. Types of immunity- Humoral and cell mediated, Cytokines and their functions.
CIA – 25
ESE – 75
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UNIT-III
Quantitative study of antigen- antibody reactions – Agglutination – Hemeagglutination,
Bacterial agglutination and precipitation – Immunodiffusion and Immunoelectrophoresis.
ELISA, Radioimmunoassay (RIA) – monoclonal antibodies and its applications (Hybridoma
Technology). Immunohematology - Blood transfusion - ABO grouping - Rh factor.
UNIT-IV
Allergy and hypersensitivity - classification types and Mechanisms - Autoimmune disorders:
systemic and organ specific disorders. Immunodeficiency diseases – SCID and AIDS.
Vaccines - Killed, Attenuated, Subunit and DNA vaccines.
UNIT-V
Major Histocompatibility Complex (MHC): types, - Class I, II and III distribution and
function. Tissue transplantation - HLA typing - mechanism of acceptance and rejection.
Tumor immunology- tumor antigens, role of NK cells in tumor evasion, cancer
immunotherapy.
TEXT BOOKS:
1. Kuby Immunology - Thomas J. Kindt, Richard A. Goldsby, Barbara A. Osborne
(2006) 6th ed. W.H. Freeman & Company, New York.
REFERENCE BOOKS:
1 Roitt’s Essential Immunology - Peter J. Delves, Seamus J. Martin, Dennis R. Burton
and Ivan M. Roitt, (2017) 13th ed. Blackwell Scientific Publications, Oxford.
2 Fundamentals of Immunology – William E Paul (2012) Raven Press, New York
3 Cellular and Molecular Immunology - Abul K. Abbas, Andrewtt, Lichtman &
Jordan S. Pober (2017) W.B. Saunder’s Company, A Harcourt Health Sciences
Company, NY.
4 Immunology - Tizard (1995) 4th ed. Saunders College Publishers, New York.
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SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective-I: Group A – Food and Dairy Microbiology
NUMBER OF HOURS/WEEK: 4 HRS
SUBJECT DESCRIPTION
This subject provides an overview of food related microbes and its interaction with food as
substrate, food spoilage and food-borne illness manifested by food borne microbes, dairy
products and its fermentation.
GOALS
To cover the fundamental aspects of Food Microbiology including food spoilage, illness and
dairy product fermentation.
OBJECTIVES
Students would be aware of factors influencing microbial growth in food, different source of
microbes in food spoilage and food-borne illness, and would acquire an overview in
fermentation process of dairy products.
CONTENTS
UNIT-I
Food and microorganisms - Important microorganism in food (Bacteria, mold and yeast)-
Factors affecting the growth of the microorganisms in food- pH, moisture, OR potential,
nutrient content, inhibitory substances and biological structures.
UNIT-II
Principles of preservations- general principles and application methods - asepsis - technique
of removal - use of temperature (low and high). Drying, radiation and chemical preservatives
(Benzoate, propionate, sodium chloride and sugar).
CIA – 25
ESE – 75
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UNIT-III
Spoilage of food: Cereal products, vegetables and fruits, eggs and milk- canned foods.
UNIT-IV
Fermented foods - Oriental foods- Soy sauce, misu, tempeh, sufu, and angkak. Fermented
dairy products- Acidophilus milk, cultured butter milk, yoghurt, kefir, koumiss and cheese
production and its types (blue veined cheese and cheddar cheese).
UNIT-V
Food borne diseases- Food borne infections and food intoxications - bacterial and
mycotoxins- investigations of food poisoning outbreak.
TEXTBOOKS:
1. Food Microbiology – Frazier, WC, Westhoff, DC. (2003) TATA Mc Graw Hill, New
Delhi
REFERENCE BOOKS:
1. Food Microbiology – Adams, MR, and Moss, MO. (2005)1st ed. Reprinted, Published
byNew Age International (P) Limited. Publishers, New Delhi.
2. Modern Food Microbiology – James M. Jay (2000) 4th ed. CBS Publishers &
Distributors, New Delhi.
3. Basic Food Microbiology – Banwart, GJ, (2004) 2nd ed. CBS Publishers &
Distributors, New Delhi.
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SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective-I: Group B – Parasitology
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
This course emphasizes on the importance of medical parasitology.
GOALS
To study the morphological characteristics for identification of parasites to taxonomic group
and the life history stage.
OBJECTIVES
On successful completion of this course, the student should Understand the evolution of
parasitic groups, as well as other aspects of the parasites discussed.
CONTENTS
UNIT-I
Introduction to Medical parasitology - classification, host-parasite relationships. Types of
infections-methods of infection. Epidemiology, life cycle, pathogenic mechanisms, lab
diagnosis, treatment of parasites.
UNIT-II
Morphology, life cycle and pathogenecity, lab diagnosis and treatment of Protozoa causing
human infections – Entamoeba histolytica, Toxoplasma gondii, Cryptosporidium,
Leishmania, Trypanasoma, Giardia, Trichomonas and Balantidium.
UNIT-III
Morphology, life cycle and pathogenicity, laboratory diagnosis and treatment of: Helminths:
Cestodes – T. solium, T. saginata and T. echinococcus.
CIA – 25
ESE – 75
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UNIT-IV
Morphology, life cycle and pathogenicity, laboratory diagnosis and treatment of – trematodes-
Fasciola hepatica, Fascio lopsisbuski, Paragonimus and Schistosomes.
UNIT-V
Nematodes: Ascaris, Ankylostoma, Trichuris, Trichinella, Enterobius, Strongyloides and
Wuchereria.
TEXT BOOKS:
1. Text book of Medical Parasitology – Protozoology and Helminthology. Subhash
Chandra Parija, 2004. 2nd edition, published by All India Publishers and Distributors,
Medical book publisher, New Delhi.
2. Text book of Medical Parasitology - JeyaramPaniker, 2004. 5th edition, JAYPEE
brothers, Medical Publishers (P) Ltd, New Delhi.
REFERENCE BOOKS:
1. Medical Parasitology - Ichpujani, R.L. and Rajesh Bhatia, 2003. 3rd edition, JAYPEE
brothers, Medical publishers (P) Ltd, New Delhi.
2. Diagnostic Microbiology - Bailey and Scott’s., 1990. Eighth edition. The Mosby
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SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective-I: Group C – Biomolecules
NUMBER OF HOURS/WEEK: 4 Hrs
SUBJECT DESCRIPTION
This course emphasizes on the biomolecules and their importance.
GOALS
To make the student to learn and understand the chemistry of biomolecules
OBJECTIVES
On successful completion of this course, the student must have gained the knowledge
regarding individual biomolecules and its importance.
CONTENTS
UNIT-I
Carbohydrates: Classification, structure, properties & chemical reactions of
monosaccharides and disaccharides (Maltose, Lactose and Sucrose). Polysaccharides -
Structure & biological functions of Homo & Hetero polysaccharides (Starch and Glycogen).
UNIT-II
Lipids: Definition, classification of lipids, physiochemical properties. Storage lipids - fatty
acids, triglycerides. Structural lipids - phospholipids, glycolipids & sphingolipids. Structure &
Biological role of cholesterol, prostaglandins, thromboxanes and leukotrienes.
UNIT-III
Classification of amino acids, general properties, Non-protein amino acids. Peptide bond -
structure & conformation, Ramachandran Plot. Protein classification, Physiochemical
properties of proteins. An over view on the organization of protein Structure – Primary,
Secondary, Tertiary, and Quaternary structure.
CIA – 25
ESE – 75
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UNIT-IV
Structures of Purines, pyrimidines, Nucleosides & Nucleotides. Properties of nucleic acids.
DNA- Double helical structure - Isoforms. RNA - Types & functions.
UNIT-V
Minerals in biological system & their importance - Iron, Calcium, Phosphorous, Iodine,
Copper and Zinc. Vitamins: Definition, classification - Fat-soluble (Vitamins A, D, E, K) and
water soluble vitamins (Vitamin B & C) - Sources, functions and deficiencies.
TEXT BOOKS:
1. Fundamentals of Biochemistry - J. L. Jain, S. Chand publications 6th edition.
2. Principles of Biochemistry – Lehninger, W. H. Freeman & Comp New York. 5th
edition
REFERENCE BOOKS:
1. Biochemistry – Zubay, 4th edition William. C. Brain publishers.
2. Biochemistry – Voet & Voet, 4th edition John Wiley & Sons (Asia) pvt ltd.
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SEMESTER – V
COURSE: B.SC., MICROBIOLOGY
SUBJECT TITLE: Skill Based Subject 3 – Clinical Laboratory Technology-I
NUMBER OF HOURS/WEEK: 3 Hrs
SUBJECT DESCRIPTION
This paper deals with the basic hospital laboratory techniques
GOALS
The goal of this subject is to provide students with a working knowledge in Clinical
biochemical techniques.
OBJECTIVES
On completion of this subject the students will gain knowledge on the clinical assessment of
various diseased conditions & Routine test followed in the laboratory.
CONTENTS
UNIT-I
Laboratory Safety: An overview of Laboratory maintenance – documentation and quality
control – introduction of biological samples – preparation of the reagents and storage –
maintenance and handling of equipment – cleaning of glass ware – documentation of the
samples and results.
Collection, Transport and analysis of Specimen: Blood, Urine, Faeces, Sputum, semen,
CSF.
Disposal of Laboratory/Hospital Waste: Non-infectious waste, Biomedical wastes, infected
sharp waste disposal, infected non-sharp waste disposal- colour coding as per guidelines.
UNIT-II
Haematology: Anticoagulants, Separation of serum and plasma. Enumeration of formed
elements: RBC Count - WBC counts - Total and differential - Platelet count. Haemoglobin
CIA – 20
ESE – 30
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estimation – Sahli’s method, Erythrocyte sedimentation rate - Westergen’s method,
Hematocrit- Wintrobe’s method, Reticulocyte count - Preparation of blood films - Stains used
in Haematology-Wright’s stain, Giemsa stain.
Clinical Chemistry: Blood sugar Analysis- glucometer based analysis, Fasting, Random,
Post Prandial, HbA1C, and GTT.
UNIT-III
Routine Analysis of Urine: Collection preservation, Color, odour, volume, Specific gravity
& pH Test for protein – Coagulation of Protein, Bence Jones protein test. Glucose –
Benedict’s qualitative test and Fehling’s test. Ketone Bodies test – Rothera’s test and
Gerhardt’s test. Bile salt - Hay’s test. Bile Pigment – Fouchet’s Test and Smith’s test.
Urobilin – Bogomolow’s test, Urobilinogen – Ehrlich’s aldehyde test. Microscopical
examination of Urine.
UNIT-IV
Histopathology: Introduction to histopathology. - Receiving Specimens in Laboratory -
Grossing techniques. Various fixatives - Mode of action, Indications, Preparation,
Decalcification of calcified tissue before sectioning - Processing of tissues for routine paraffin
sections and other methods of embedding (histopathological examination of pathogenic
sample-nephrotic syndrome).
UNIT-V
Blood Banking: Blood Group (ABO & Rh) – methods of grouping & reverse grouping. Basic
blood banking procedures – collection of blood, anticoagulants used, cross matching, different
screening tests including Coomb’s Test (direct and indirect), preparation of different blood
components for use and how to serve a requisition. Preparation of red cell suspension. Blood
transfusion & hazards.
REFERRENCES
TEXT BOOKS:
1. Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl
and Edward R. Ashwood, David E. Burns, 4th edition, (2005), W.B. Saunders
Company.
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2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006, Jaypee Brothers
Medical Publishers (P) Ltd.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005), 4th Edition, CBS Publishers
and Distributors.
2. Medical Laboratory Technology Vol.1 Kanai L. Mukherjee, 2010, Tata McGraw Hill
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SEMESTER – V
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Practical III: Medical, Industrial And Agricultural
Microbiology.
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
These practical gives a representation of Medical Microbiology Industrial and Agricultural
Microbiology.
GOAL
These practical helps the student the practical knowledge of various molecular techniques and
also helps in understanding the processing techniques of a clinical sample
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of a
medically important pathogen and also various techniques involved in industries and in
agricultural field.
CONTENTS
I. MEDICAL MICROBIOLOGY
1. Collection and Processing of clinical specimens and identification of pathogens
from clinical specimen
a. Urine
b. Blood
c. Pus
d. Throat swab
II. ANTIBIOGRAM FOR THE CLINICAL ISOLATES
2. Kirby bauer technique
III. HAEMATOLOGY
3. Identification of WBC –Basophils, Eosinophils, Neutrophils
CIA – 40
ESE – 60
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4. Blood grouping test
IV. SEROLOGY
5. WIDAL
6. RPR
7. ASO
8. CRP
9. Immunodiffusion –Radial and Double Immunodiffusion (ODD)
10. Immuno electrophoresis
V. INDUSTRIAL MICROBIOLOGY
Production and estimation of
11. Microbial enzymes – Amylase and Protease
12. Wine
VI. ENVIRONMENTAL, FOOD AND AGRICULTURAL MICROBIOLOGY
13. Analysis of the spoilers
Vegetables and fruits,
Beverages –soft drinks
Ice-cream
14. MBRT –milk quality testing
15. Water potability test -MPN
16. Isolation of nitrogen fixing microorganisms – symbiotic and free living
17. Isolation of Phosphate solubilizers
18. Isolation of fungal phytopathogens
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REFERENCES:
1. Experiments in Microbiology plant pathology and biotechnology – Aneja, KR.
(2003) 4th ed. New age international publ.
2. Microbiology – Laboratory Manual - James G. Cappuccino and Natalie Sherman
(1999) 6th ed. Benjamin/Cummings Science Publishing.
3. Microbiology Manual – Kannan, N, (2000).Palani Paramount Publishing.
4. Experimental procedures in Lifesciences - Rajan, S. (2010) Anjana book house.
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SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper XI: Medical Microbiology – II
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
Medical microbiology is a branch of medicine concerned with the prevention, diagnosis and
treatment of infectious diseases. In addition, this field of science studies various clinical
applications of microbes for the improvement of health.
GOALS
To gain knowledge on the aspects of the interrelationship between infectious agents and their
hosts.
OBJECTIVES
On successful completion of the subject, the student will know about the mechanism of
infection and its types & provides the information about the prevention of diseases.
CONTENTS
UNIT-I
Laboratory methods in basic Mycology- Collection and transport of clinical specimens-
Direct Microscopic examination, Culture media and incubation, Serological tests for fungi -
Antifungal susceptibility testing.
UNIT-II
Medical Mycosis - Identification, and Transport of fungal specimens - pathogenesis of
superficial mycosis - Tinea, Piedra. Cutaneous mycosis - Dermatophytosis. Subcutaneous
mycosis - Sporotrichosis, Mycetoma, Systemic mycosis - Blastomycosis and Histoplasmosis.
Opportunistic mycosis - Candidiasis, Cryptococcosis and Aspergillosis.
UNIT-III
Introduction to parasitology: Types of parasite and host. Lab diagnosis- Clinical and
parasitic. Diagnosis of parasitic Infections-Collection, processing and examination of
CIA – 25
ESE – 75
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specimen from stool, sputum, cerebrospinal fluid and duodenal content. Examination of blood
for blood parasites.
UNIT-IV
Morphology, life cycle, pathogenicity and lab diagnosis of Entamoeba histolytica, Giardia
intestinalis, Plasmodium falciparum, Toxoplasma gondii and Taeniasaginata.
UNIT-V
Morphology, life cycle, pathogenicity and lab diagnosis of Trichuris trichura, Ancylostoma
duodenale, Enterobius vemiculari, Ascaris lumbricoides and Wuchereria bancrofti.
TEXT BOOKS:
1. Introduction to the History of Mycology - Ainsworth (2009), Cambridge University
Press
2. Text book of Medical Parasitology – Protozoology and Helminthology. Subhash
Chandra Parija, 2004. 2nd edition, published by All India Publishers and Distributors,
Medical book publisher, New Delhi.
3. Text book of Medical Parasitology – Jeyaram Paniker, 2004. 5th edition, JAYPEE
brothers, Medical Publishers (P) Ltd, New Delhi.
REFERENCE BOOKS:
1. An introduction to Mycology - Mehrotra, R.S. and Aneja, K.R., 2006. Reprinted and
Published by New Age International (P) Ltd, Publishers, New Deli.
2. A Text Book of Medical Mycology – Jegadish Chander, 1996. Interprint, New Delhi.
3. Medical Parasitology - Ichpujani, R.L. and Rajesh Bhatia, 2003. 3rd edition, JAYPEE
brothers, Medical publishers (P) Ltd, New Delhi.
4. Diagnostic Microbiology - Bailey and Scott’s., 1990. Eighth edition. The Mosby
Company.
5. Medical laboratory techniques - Abdul Khader, 2003, First edition. Frontline
Publications, Hyderabad.
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SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Paper XII: Industrial Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of Industrial microbiology, Upstream and Downstream process
used in the production of Primary and Secondary Metabolites.
GOAL
To make the Students to understand the fundamental processes involved in upstream and
down streaming process in the productions of microbial products.
OBJECTIVE
Students on completion of this paper will acquire a thorough knowledge on schematic process
of fermentation techniques and product development.
CONTENTS
UNIT-I
Industrially important strains- Screening methods - Strain development for improved yield -
Mutation, Recombination and protoplasmic fusion.
UNIT-II
Fermentors: Component parts of CSTR – Types of Fermenter (tower, cylindroconical &
airlift) – Fermentation: Submerged and solid state- Batch fermentation- Continuous
fermentation.
UNIT-III
Fermentation media formulation - Inoculum development for bacterial and mycelial
processes- oxygen transfer -Newtonian fluid and non-Newtonian fluids sterilization of media -
air.
CIA – 25
ESE – 75
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UNIT-IV
Downstream process - intracellular and extra cellular products, liquid- liquid extraction,
precipitation, filtration, centrifugation, solvent extraction, ion exchange resin and drying.
UNIT-V
Production of beverages - beer and wine - vitamin B12 and riboflavin- antibiotics- penicillin
and streptomycin -Bio fuel: Production of Bio ethanol from sugar Molasses, Starch and
cellulosic materials, ethanol recovery.
TEXT BOOKS:
1. Industrial Microbiology - Patel, A.H. (2005) Macmillan India Ltd, New Delhi.
2. Industrial Microbiology - Casida, J. R. (2005) New Age International (P) Limited,
New Delhi.
REFERENCE BOOKS:
1. Industrial Microbiology - Samuel C Presscott and Ceril G Dunn (2005) Agrobios
(India), Agro house, Jodhpur.
2. Microbial Technology - Peppler, H.J. and Pearlman, D. (2004). 2nd edition, Academic
press.
3. Principles of Fermentation Technology – Stanbury A. Whitaker and S.J. Hall
(1997). 2nd edition, Pergamon press.
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SEMESTER-VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Core Practical III: Medical, Industrial and Agricultural
Microbiology.
NUMBER OF HOURS/WEEK: 5 Hrs
Refer Semester V Core Practical III for syllabus
CIA – 40
ESE – 60
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SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective-II: Group A – Environmental and Agricultural
Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper brings in the importance of soil microorganisms, its interactions and the role of
microorganisms in the environment in cycling of elements.
GOALS
To make the students to understand the importance of soil microorganisms and its role in the
mineralization of compounds. It also adds the knowledge of the role of microbes in the
environment.
OBJECTIVES
On successful completion of the course the students understand the various role of
microorganisms in the environment and agriculture.
CONTENTS
UNIT-I
Soil environment Microbial communities in soil - bacteria, actinomycetes, algae, fungi, virus,
protozoans & nematodes and their significance. Microbial associations – symbiotic proto
cooperation, ammensalism, commensalisms, syntropism, parasitism and predation with
suitable examples.
UNIT-II
Biogeochemical cycling of compounds- Carbon cycle- Nitrogen cycle - nitrogen fixing
microorganisms - Root nodule bacteria - non symbiotic nitrogen fixers - Phosphorous cycle.
Mycorrhizae -Ectomycorrhiza, Endomycorrhiza & Ectendomycorrhizae.
CIA – 25
ESE – 75
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UNIT-III
Biofertilizers: Mass production of Bacterial biofertilizers (Rhizobium and Phosphate
solubilizing bacteria) - cyanobacterial biofertiliser and fungal (mycorrhizae) biofertilizers -
their significance and practice. Microbial decomposition; Cellulose, hemi cellulose, lignin,
pectin and chitin. Aerobic and anaerobic degradation of organic matter.
UNIT-IV
Aero Microbiology- aerosol, droplet nuclei, air pollution- sources- (Microbiological) – air
quality analysis- air sampling devices. Air borne diseases.
UNIT-V
Aquatic Microbiology- Algae - Phytoplankton – Eutrophication - Distribution of
Microorganisms in different water systems – Factors influencing the distribution of
Microorganisms. Waste water recycling - Primary, secondary and tertiary treatment.
Potability of drinking water - MPN technique.
TEXT BOOKS:
1. Environmental Microbiology - Vijaya Ramesh K (2004). 1st Edition, MJP Publishers
(A Unit of Tamil Nadu Book House) Chennai.
2. Environment Aspects of Microbiology - Joseph C Daniel (1999). 1st Edition, Bright
sun Publications, Chennai.
REFRENCE BOOKS:
1. Biofertilizers in Agriculture and Forestry - Subbarao NS 1995. 3rd edition,
Oxfordand IBH pub, co. pvt ltd, NewDelhi.
2. Agricultural Microbiology - Rangaswami G & DG Bagyaraj , 2002. 2nd Edition,
Prentice- Hall of India, New Delhi.
3. Microbial Ecology - Atlas RN & Bartha R 1998, 4th edition, Benjamin cummings.
4. Introduction to soil Microbiology - Alexander M 1977. John Wiley & sons, Inc.
Newyork.
5. Soil Microbiology - Subba Rao NS 2004, 4th edition, Oxford & IBH
PublishingCo.pvt.Ltd., NewDelhi.
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SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective - II: Group B – Medical Biochemistry
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This course emphasizes on the importance of medical Biochemistry.
GOALS
To make the student to learn and understand the chemistry of biomolecules
OBJECTIVES
On successful completion of this course, the student must have gained the knowledge
regarding individual biomolecules and its importance.
CONTENTS
UNIT-I
Disorders of carbohydrate metabolism – Diabetes mellitus, Glucose tolerance tests, sugar
levels in blood, renal threshold for glucose, factors influencing blood glucose level, glycogen
storage diseases, pentosuria and galactosemia.
UNIT-II
Disorders of lipids- Plasma lipoproteins, cholesterol, triglycerides and phospholipids in health
and disease, hyperlipidemia, hyperlipoproteinemia, Gaucher’s disease, Tay-Sach’s, ketone
bodies and ß-lipoproteinemia.
UNIT-III
Disorders of liver and kidney- Jaundice, fatty liver, normal and abnormal functions of liver
and kidney, inulin and urea clearance.
CIA – 25
ESE – 75
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UNIT-IV
Abnormalities in nitrogen metabolism-Uremia, hyperurecemia, porphyria and factors
affecting nitrogen balance.
UNIT-V
Blood - composition and functions, properties and functions of haemoglobin. Blood Clotting-
disturbances in blood clotting mechanisms- haemorrhagic disorders, haemophilia, purpura,
thrombocytopenic purpura, disseminated intravascular coagulation, acquired prothrombin
complex disorders, circulating anticoagulants.
TEXT BOOKS:
1. Fundamentals of biochemistry. A. C. Deb. 8th edition. New central book agency (p)
ltd. India
2. Biochemistry. U. Sathyanarayana. 2nd edition. Books and allied pvt ltd.
REFERENCE BOOKS:
1. Textbook of biochemistry with clinical correlations. Thomas M Deblin. 4th edition.
John Wiley and sons, Inc., publications., New York.
2. Fundamental of Biochemistry for medical students. Ambika Shanmugam. Revised
Edition (2003). Published by the Author, 17. III Cross street, west CIT nagar,Chennai-
35
121 Director, SBS
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Page No.44
SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective-II: Group C – Plant Biotechnology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This course emphasizes on the importance of Plant Biotechnology.
GOALS
To make the student to learn and understand the concepts of plant tissue culture and its
importance.
OBJECTIVES
On successful completion of this course, the student must have gained the knowledge on
how to prepare the plantlets and handling procedures.
CONTENTS
UNIT-I
Plant Tissue culture – History, Plant tissue culture media, types, constituents and preparation
of media, selection of suitable medium.
UNIT-II
Protoplast culture and somatic hybridization, production of Haploid plants, Somaclonal
variations, Clonal propagation (micro propagation) germplasm conservation and
cryopreservation.
UNIT-III
Genetic Engineering of plants - Gene transfer methods – vector mediated gene transfer, virus -
mediated gene transfer, Direct or Vectorless DNA transfer.
CIA – 25
ESE – 75
122 Director, SBS
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Page No.45
UNIT-IV
Application of Transgenic plants – Resistance to biotic stresses – Insect resistance plant virus,
bacteria and fungi resistance, abotic stress - herbicide resistance plants.
UNIT-V
Molecular Marker Aided plant Breeding - Molecular markers, Molecular marker assisted
selection, Arid & semi-arid plant Biotech, Green house & Green home technology.
TEXT BOOKS:
1. Biotechnology. Dr. U. Sathyanarayana. Books and Allied Publications
REFERENCE BOOKS:
1. From genes to clones. Introduction to Gene Technology. Winnacker, E.L. 1989.
VCH Weinhein.
2. Principles of Gene Manipulation. Old, R,M. and S.B. Primrose. 1995. Blackwell
Scientific Publication. London.
3. Molecular Biotechnology – Principles and applications of recombinant DNA.
Glick, B.K. and Pasternak, J.J. 1994. ASM Press, Washington.
123 Director, SBS
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Page No.46
SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective III: Group A – Pharmaceutical Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of enzyme and antimicrobial products production related to
pharmacy. The information about antibiotics and their mode of action indicates the usage of
advancement in pharmacy research.
GOAL
This paper in microbiology has been designed to provide the student with a firm foundation in
the pharmaceutical aspects of microbiology, which forms a base for their future research. This
paper also provides the basics in microbial spoilage and sterilization of pharmaceutical
products.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of
production aspects of pharmaceutical important enzymes, products and diagnostic aids.
CONTENTS
UNIT-I
Antibiotics and synthetic antimicrobial agents (Aminoglycosides, β-lactams, tetracyclines,
ansamycins, acrolid antibiotics) Mechanism of action of antibiotics (inhibitors of cell wall
synthesis, nucleic acid and protein synthesis) Antifungal antibiotics, antitumor substances.
Peptide antibiotics, Chloramphenicol, Sulphonamides and Quinolinone antimicrobial agents.
Chemical disinfectants, antiseptics and preservatives.
UNIT-II
Types of microbial spoilage, Factors affecting the microbial spoilage of pharmaceutical
products, Sources and types of microbial contamination, Microbial standards for non-sterile
CIA – 25
ESE – 75
124 Director, SBS
Anx.UMb 11,3B.Sc. MB (CMS College) 2017 2015-18 batch
Page No.47
pharmaceuticals, Assessment of microbial contamination and spoilage, Microbial limit tests,
Preservative efficacy test: challenge test
UNIT-III
Sterilizing pharmaceutical products, Different methods - dry heat, moist heat, gaseous,
radiation and filtration. Sterilization monitors, D-value, Z-value, Sterility testing of
pharmaceutical products.
UNIT-IV
Chemical classification of different disinfectants, dynamics and factors affecting on
disinfectant action and selection of disinfectants, methods used for evaluation of disinfectant,
Phenol coefficient test.
UNIT-V
Manufacturing procedures and in process control of pharmaceuticals. Other pharmaceuticals
produced by microbial fermentations (streptokinase, streptodornase). New vaccine
technology, DNA vaccines, synthetic peptide vaccines and multivalent subunit vaccines.
TEXT BOOKS:
1. Pharmaceutical biotechnology - Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. and Russel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS:
1. Gene therapy - Templeton and Lasic (2000) Marcel and Dekkn.
2. The basic science of gene therapy – Richard C. Mulligan (1993) American
Association for the Advancement of Science.
3. Comprehensive biotechnology - Murray Moo-Young (Ed) Vol-3 (2004) Permagon
Press.
4. Pharmaceutical biotechnology - Purohit (2003) 2nd ed. Agrobios.
125 Director, SBS
Anx.UMb 11,3B.Sc. MB (CMS College) 2017 2015-18 batch
Page No.48
SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective III: Group B – Entrepreneurial Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This course emphasizes on the various aspect for the students to become a successful
entrepreneur.
GOALS
To make the student to learn the concepts of entrepreneurship.
OBJECTIVES
On successful completion of this course, the student must have gained the knowledge on self-
employment in relation to Microbiology.
CONTENTS
UNIT-I
Entrepreneur development, activity, Institutes involved, Government contributions to
entrepreneurs, risk assessment. Industrial Microbiology, Definition, scope and historical
development.
UNIT-II
Microbial cells as fermentation products- Bakers yeast, food and feed yeasts, Bacterial
Insecticides, Legume Inoculants, Mushrooms, Algae. Enzymes as fermentation products-
Bacterial and Fungal Amylases, Proteolytic Enzymes, Pectinases, Invertases, and other
enzymes.
UNIT-III
Mushroom cultivation and Composting- Cultivation of Agaricuscampestris,
Agaricusbisporus, and Volvariellavolvaciae; Preparation of compost, filling tray beds,
CIA – 25
ESE – 75
126 Director, SBS
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Page No.49
spawning, maintain optimal temperature, casing, watering, harvesting, storage. Biofertilizers-
Historical background, Chemical fertilizers versus biofertilizers, organicfarming. Rhizobium
sp, Azospirillum sp, Azotobacter sp, as Biofertilizers.
UNIT-IV
Patents and secret processes, History of patenting, composition, subject matter and
characteristics of a patent, Inventor, Infringement, cost of patent. Patents in India and Other
countries.
UNIT-V
Brewing- Media components, preparation of medium, Microorganisms involved, maturation,
carbonation, packaging, keeping quality, contamination, by products. Production of Industrial
alcohol.
TEXT BOOKS:
1. Entrepreneurial Development in India – Arora
REFERENCE BOOKS:
2. Industrial Microbiology – L. E. Casida, Jr, New age International publication.
3. Experiments in Microbiology, Plant pathology, Tissue culture and Mushroom
production technology – K. R. Aneja, New age International publication.
127 Director, SBS
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Page No.50
SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: Elective III: Group C – Bionanotechnology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
The paper describes about the study of the controlling of matter on an atomic and molecular
scale. Nanotechnology is a find that turned the world around.
GOALS
To gain knowledge about the nanoparticles of various sizes and their biological importance.
OBJECTIVES
On successful completion of the subject, the student will know about the projected ability to
construct items from the bottom up, using techniques and tools being developed today to
make complete, high performance products.
CONTENTS
UNIT-I
Introduction- Definition, about Bionanomachines, Molecular Bionanotechnology. History of
Bionanotechnology; Richard Feynman and his contributions. Biotechnology versus
Bionantechnology. Natural Bionanamachines.
UNIT-II
Structural Principles of Bionanaotechnology- Environment in which the Bionanomachines
Functions. Principles behind design of Natural Bionanaomachines- Covalent bonding,
Dispersions and repulsion forces. Hydrogen bonding, Electrostatic Interaction, Hydrophobic
effect. Hierarchical strategy in construction of Bionanomachines – Selfassembly, Self-
organization.
CIA – 25
ESE – 75
128 Director, SBS
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Page No.51
UNIT-III
Functional Principles of Bionanotechnology- Information storage- Nucleic acid, Ribosomes
as assembler to construct proteins. Energetics- Energy from Light, electrontransport
pathways, electrochemical gradient. Biocatalysts- Enzymes and its regulation. Biomaterials.
Biomolecular motors. Molecular sensing- Biosensors.
UNIT-IV
Tools and technique required for Bionanaotechnology- Recombinant DNA technology; site
directed mutagenesis, Fusion proteins. X-Ray Crystallography, NMR, Electron Microscopy,
Atomic force Microscopy. Bioinformatics- Molecular Modeling, Docking, Computer assisted
Molecular design.
UNIT-V
Applications of Bionanotechnology- Nanomedicines; Immunotoxins, Liposomes as drug
carriers, Gene therapy, Personalised Medicines; Lab on chip concept. DNA Computers,
Artificial Life, Hybrid materials, Biosensors.
TEXT BOOKS:
1. Bionanotechnology – Goodsell
REFERENCE BOOKS:
1. Nanomedicines. Parag Diwan and Asish Bharadwaj. Pentagan Press, 2006.
2. Nanoparticles as Drug Carriers – Vladimir P Torchilin,. Imperial College Press,
North Eastern University, USA. 2006.
129 Director, SBS
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Page No.52
SEMESTER – VI
COURSE: B.Sc., Microbiology
SUBJECT TITLE: SBS –IV– Clinical Laboratory Technology-II
NUMBER OF HOURS/WEEK: 3 Hrs
SUBJECT DESCRIPTION
This paper deals with the advanced diagnostic methods for microbial infections.
GOALS
This subject is extremely important for the student as it paves the ways to easily understand
various professional courses such as parasitology, mycology and others.
OBJECTIVES
On completion of this subject the students will be of well trained, qualified, competent and
task oriented medical laboratory technologists.
CONTENTS
UNIT-I
Laboratory techniques in Bacteriology: Pathogenic organisms- Laboratory diagnosis of
UTI, Sore throat, diarrhoea, acute pyogenic meningitis, Food poisoning. Methods for Testing
Antimicrobial Susceptibility-Kirby Bauer Disc Diffusion method, MIC determination, ESBL
testing.
UNIT-II
Laboratory techniques in Mycology: Collection, handling & processing of clinical
mycology specimens: Direct examination of specimens: i) Saline Wet Mount, ii) KOH
Preparation. Stains: i) Lactophenol cotton blue and ii) Acid-Fast Stains. Fungal Culturing:
Selective media for isolation.
CIA – 25
ESE – 75
130 Director, SBS
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Page No.53
Laboratory techniques in Virology: Direct detection (Electron Microscopy with one
example), virus isolation (Cytopathic effect with one example), serological identification with
one example (ELISA-Hbs-Ag)
UNIT-III
Laboratory techniques in Parasitology: Identification of Parasite, nematode & protozoa,
Occult blood test. Examination of faeces for ova and cysts – worm burden, concentration
methods. Floatation and sedimentation techniques. Staining by iron haematoxylin method,
blood smear examinations (thick / thin smear) – cultivation of protozoan parasites.
UNIT-IV
Clinical Biochemistry: Liver function test – SGOT, SGPT, ALP, ACP, LDH, bilirubin.
Renal function test - Glomerular Filtration Rate (GFR), Clearance test – Urea, Inulin
Clearance & Creatinine Clearance. Fish berg concentration and dilution test.
UNIT-V
Laboratory diagnosis of gastrointestinal system:
Gastric function test: Introduction, The insulin stimulation test, determination of Gastrin in
serum and Tubeless gastric analysis.
Pancreatic Function: Introduction, serum amylase and lipase.
Intestinal function: Introduction, test of monosaccharide absorption (xylose excretion test)
and determination of total protein (Lowry’s method).
TEXT BOOKS:
1. Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl
and Edward R. Ashwood, David E.Burns, 4th edition, (2005), W.B. Saunders
Company.
2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006, Jaypee Brothers
Medical Publishers (P) Ltd.
131 Director, SBS
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Page No.54
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical laboratory technology Vol.1 Kanai L. Mukherjee, (2007),.Tata McGraw
Hill
3. Advanced techniques in Diagnostic Microbiology-Yi-Wei Tang, Charles W.
Straston, 2006, Springer.
4. Practical Biochemistry Principles and Techniques-Wilson K, Walker J. 2nd Edition
(2000), Cambridge University Press.
132 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS) Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
I Year M.Sc., Microbiology
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS)
(2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017)
133 Director, SBS
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Page No.2
DEPARTMENT OF MICROBIOLOGY
VISION OF THE DEPARTMENT
To produce competent professionals in every field of Microbiology by providing inspirational
learning process to complement our national needs and to be recognized as a Centre of
Excellence in Research, Innovation, Consultancy and Entrepreneurship.
MISSION OF THE DEPARTMENT
To provide facilities and offer opportunities for graduate and post-graduate education in
microbiology through advanced instruction/teaching methodologies, quality training and
innovative research;
To prosecute and promote research in microbiology for the advancement of knowledge
and for the betterment of society.
To serve as a centre for fostering co-operation and exchange of ideas between the
academic, research and industrial sector.
M.Sc., MICROBIOLOGY COURSE
PROGRAM EDUCATIONAL OBJECTIVES
PEO1: To acquire basic knowledge on core concepts in diverse field of Microbiology.
PEO2: To inculcate practical training on basic techniques and processes in Microbiology.
PEO3: To impart skill-based education for entrepreneurial development.
PEO4: To adapt with the continuously evolving technologies in industry.
After completion of the program the graduates will be able to
PO1: Execute the knowledge in identifying problems and to develop a solution.
PO2: improve analytical and technical skills to enhance employment potential.
PO3: Implement the skill set to become a successful entrepreneur.
PO4: Acquire a holistic professional career in hospitals, food and pharmaceutical industries.
134 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.3
MAPPING OF PEO’S AND PO’S
PEO1 PEO2 PEO3 PEO4
PO1
PO2
PO3
PO4
DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT@ No of Papers Marks
@ Credits
I Subjects and Practicals$ 17 1700 68
II Elective Subjects 4 400 16
III Project – 100 4
IV Industrial/Institutional Training** – 50 2
Total 22 2250 90
Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects respectively.
$ In core subjects both theory and practical’s are included wherever applicable.
The following parameters are considered throughout study period.
i. Regularity of Attendance
ii. Active participation in classes/Camps/Games (College/District//University)
iii. Exemplary awards/certificates/prizes
iv. Other Social Components (Blood Camp, Fine Arts, etc)
Note: II
The Credit points and Lecture Hours are not linked.
135 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.4
Annexure No. PMB1 BOS.DT:12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049
SCHOOL OF BIOLOGICAL SCIENCES
M.Sc., MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017)
Sem
este
r
Par
t
Su
bje
ct C
ode
Subject Title
Inst
. H
rs p
er w
eek
Examination Details
Du
rati
on i
n
Hou
rs.
CIA
En
d S
em
Exa
m
Tot
al M
ark
s
Cre
dit
s
SEMESTER I
I
Paper I Fundamentals of Microbiology 5 3 25 75 100 4
Paper II Microbial physiology and
Metabolism 5 3 25 75 100 4
Paper III Food and Dairy Microbiology 5 3 25 75 100 4
Paper IV Molecular Genetics 5 3 25 75 100 4
Practical-I
Practical-I Microbial
physiology, Molecular genetics
and Food microbiology
5 9 40 60 100 4
Elective-I Elective I 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER II
II
Paper V Environmental Microbiology 5 3 25 75 100 4
Paper VI Fermentation Microbiology 5 3 25 75 100 4
Paper VII Recombinant DNA Technology 5 3 25 75 100 4
136 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.5
Paper VIII Research Methodology and
Bioanalytical Techniques 5 3 25 75 100 4
Practical-II
Practical II: Environmental
Microbiology, Fermentation
Microbiology and Recombinant
DNA Technology
5 9 40 60 100 4
Elective-II Elective II 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER III
III
Paper-IX Medical Microbiology 5 3 25 75 100 4
Paper-X Virology 5 3 25 75 100 4
Paper-XI Immunology and
Immunotechnology 5 3 25 75 100 4
Paper-XII Genomics and Proteomics 5 3 25 75 100 4
Practical-III Practical III: Medical
Microbiology and Immunology 5 9 40 60 100 4
Elective-III Elective III 5 3 25 75 100 4
Industrial / Institutional
training. - - - - 50 2
Total 30 - 165 435 650 26
SEMESTER IV
IV
Paper-XIII Soil and Agricultural
Microbiology 5 3 25 75 100 4
Practical-IV Practical IV: Soil and
Agricultural Microbiology 5 9 40 60 100 4
Elective-IV Elective IV 5 3 25 75 100 4
Project 15 - 60 40 100 4
Total 30 - 145 250 400 16
Grand Total - - - - 2250 90
137 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.6
Each paper carries an internal component
There is a pass minimum for an external component
Theory: Internal assessment: 25 marks; External component: 75 marks
Practical: Internal assessment: 40 marks; External component: 60 marks
* Project: 2 Reviews (20+20) and Attendance (20): 60 marks; Final Viva voce: 40 marks
* The project report is the bonafide work carried out by the candidate under the guidance of a
faculty authenticated and countersigned by the Director. This project work must be presented
and defended by the candidate in the department attended by all faculties and reviewed by
external examiner.
** Industrial / Institutional training:
Each student has to undergo an Industrial / Institutional training during the summer vacation
prior to Semester III. This should be of a minimum duration of 15 working days at any off-
campus site approved by the Director. Students are required to submit three copies of
Industrial / Institutional training report with attendance certificate upon completion and the
bonafide report carried out by the candidate under the guidance of a faculty authenticated and
countersigned by the Director. A report on the Industrial / Institutional training must be
presented by the candidate in the Department (at the end of third semester) attended by all
faculty and reviewed by the respective guide as examiner.
@ Industrial / Institutional training :
Attendance and internal assessment by the guide = 30 marks (Internal)
Final Viva Voce = 20 marks (External) (Dissertation = 10 marks + Viva = 10 marks)
COMPONENTS OF INTERNAL ASSESSMENT*
Theory: Two tests in each semester (one Internal Test and one Model Test). Marks from both
Tests will be taken: Total 15 marks (5 marks Internal Test and 10 marks Model Test)
Assignments and seminar: 2.5 marks + 2.5marks = 5 marks
Overall performance: 5 marks
138 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.7
Practicals I, II, III and IV (for 40 marks):
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Practical: Marks should be awarded to each practical by the course teacher and the average
of the best ten practicals be taken for 40 marks.
Project: 60 marks should be awarded as internal assessment marks (40 marks for two
monthly reviews conducted by the project guide, course coordinator and the Director, 20
marks for attendance).
* Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge, subject in charge and course coordinator. The final
decision to be made by the Director with the approval from the Principal.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I / Sem I Bioethics, Biosafety,
TQM and IPR
Quality assessment in
pharmaceuticals
Basic biological
sciences
Paper II / Sem II Bioinformatics
Communicable and
non-communicable
disease
Biophysics and
Biochemistry
Paper III / Sem III Pharmaceutical
Microbiology
Health care of the
community
Molecular cytology
and tissue Engineering
Paper IV / Sem IV
Diagnostic
Microbiology and
Molecular
Diagnostics
Good Manufacturing/
Laboratory Practices
Computational
Molecular Biology
139 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.8
Annex EDC: PG BS BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE AND COMMERCE, (AUTONOMOUS)
COIMBATORE – 641 049.
M. Sc. Biotechnology/Biochemistry/Microbiology/Applied Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2015 onwards)
Note:
The student has to compulsorily select one Extra Disciplinary Course offered by other
departments and is eligible to get 4 extra credits. This paper is offered as self study
from the 2015 batch onwards.
Par
t
Su
b c
od
e
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m
in
hrs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
SEMESTER III
Organic farming - 3 25 75 100 4
Total - - - - 100 4
140 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.9
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049.
M.Sc., MICROBIOLOGY
MODULAR COURSE
Post-Graduate Diploma in Clinical Laboratory Technology (PG Dip. in CLT)
(For the students admitted during the academic year 2017 onwards)
(*SS – Self study paper, T – Taught programme).
Note:
The student has to undergo compulsorily a PG Diploma course offered by the
department and the student is eligible to get 2 extra credits for each paper. This PG
Diploma course consists of module 1, 2 and 3. Module 1 consists of 2 papers, Module
2 consists of 3 papers and Module 3 consists of 3 papers. Total credits of the modular
course are 16. After the successful completion of the Module I, II and III, students are
eligible to get a PG Diploma in Clinical Laboratory Technology
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ se
m
Examinations
Du
r.
Exa
m
in
hrs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Module I – Paper I – Introduction to Clinical Laboratory Technology (T*)
30 min 90 min 10 40 50 2
Module I – Paper II – Techniques in Clinical Laboratory Technology (T*)
30 min 90 min 10 40 50 2
Module II – Paper I - Human Physiology (SS*)
30 min 90 min 10 40 50 2
Module II – Paper II – Clinical Biochemistry (T*)
30 min 90 min 10 40 50 2
Module II – Paper III – Clinical Microbiology (T*)
30 min 90 min 10 40 50 2
Module III – Paper I – Endocrinology and Tumour markers (T*)
30 min 90 min 10 40 50 2
Module III – Paper II – Immunology, Serology and Parasitology (SS*)
30 min 90 min 10 40 50 2
Module III– Paper III – Molecular Diagnostics (SS*)
30 min 90 min 10 40 50 2
Total 240 - - 400 16
141 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.10
Minimum 20 days of clinical laboratory training is compulsory during Ist and IInd year.
Year Odd semester Even semester Module details
I 1st paper, 2nd paper
(Module 1) - teaching
-
1st paper (Module 2) –
Self Study
2nd paper (module 2) –
Teaching
Module 1 & 2
II 3rd paper (Module 2) –
teaching
1 paper (Module 3) –
teaching
2 paper (Module 3) –
Self Study
3 paper (Module 3) –
Self Study
Module 2 & 3
1. Upon completion of 60 hours in module – 1, the students are eligible to receive
“Certificate in Clinical Laboratory Technology” along with four extra credits.
2. Upon completion of 150 hours inclusive of module – 1 & 2, the students are eligible
to receive “Diploma in Clinical Laboratory Technology” along with two extra credits.
3. The PG students those who complete the module – 1 & 2 along with module – 3 with
a total of 90 hours are eligible to receive “Post-Graduate Diploma in Clinical
Laboratory Technology”.
4. The PG students those who have already completed the Module – 1 & 2 in their UG
stream can directly appear for module – 3 and upon completion are eligible to receive
“Post-Graduate Diploma in Clinical Laboratory Technology”.
5. Students will be eligible for credits only upon completion of entire module.
6. Instructional hours for each paper shall comprise 20 contact hours, 2 hours for end
semester examination and 8 hours of activity which may include any of the following;
Case study/field visit/lab hours/industrial training.
7. For UG certificate and Diploma programme all the papers will have contact classes.
For PG Diploma, 1 paper will be contact and other 2 papers will be self-study.
142 Director, SBS
Anx.PMB11, 1M.Sc. MB (CMS College) 2017 2017-19 batch
Page No.11
SEMESTER – I
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper I – Fundamentals of Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
The paper will provide a basic understanding of microbes, their biology, and their importance
and describe basic concepts of microbiology.
Impact/role of microorganism on life and provide a foundation for later study in biological
sciences.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Recall the important events occurred in the field of Microbiology. (K1)
CO2: Assess the phylogenetic relationship among the Microorganisms. (K5)
CO3: Understand the sterilization techniques and staining methods. (K2)
CO4: Describe the classification of Algae and Fungi. (K5)
CO5: Describe the Exploitation of Microbes. (K5)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
.
CIA – 25 ESE – 75
143 Director, SBS
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CONTENTS
UNIT-I
Historical developments of Microbiology: Contributions of Leeuwenhoek, John Needham,
Lazzaro Spallanzani, Theodar Schwann, John Tyndall, Louis Pasteur, Robert Koch,
Winogradsky (Winogradsky column), Beijerinck and Joseph Lister.
UNIT-II
Microbial Taxonomy: Whittaker’s five kingdom approach. Major characteristics used in
taxonomy – Morphological, Physiological and Metabolic, Genetic and Molecular- Numerical
taxonomy. Bergey’s manual of systematic bacteriology – first and second edition - and its
importance.
UNIT-III
Techniques in Microbiology: Methods of Sterilization - Physical and chemical agents,
radiation & filtration, Microbiological media: Enriched medium, enrichment medium,
transport medium, selective medium& pure culture techniques. Staining techniques- Simple,
Differential, Spores and Capsule.
UNIT-IV
Outline classification of Fungi and Algae: General structure and characteristics and
lifecycle: Fungi– Ascomycetes (Saccharomyces) Zygomycetes (Rhizopus) and
Deuteromycetes (Aspergillus) and Basidiomycetes (Agaricus). Algae: Chlorophyta
(Chlamydomonas) Rhodophyta (Polysiphonia).
UNIT-V
Exploitation of Microbes by Human: Microbes as sources of protein (Agaricus &
Spirullina) – Biofertiliser (Rhizobium), Biopesticide (Bt), Antibiotics (Streptomycin),
recombinant vaccines (FMD vaccine & interferons).
TEXT BOOKS:
1. Microbiology. Prescott, L.M., J.P. Harley and D.A.Klein. 1993. 2nd edition. WMC
Brown publishers.
2. Microbiology. Michael J. Pelczar Jr.1993, Concepts and Applications McGraw-Hill.
Inc.
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REFERENCE BOOKS:
1. Microbiology. An introduction. Gerald J Tortora, Berdell R Funke, Christine L Case
(1995), 5th Edition; The Benjamin Cummings Publishing Co.Inc. USA.
2. Microbiology. Principles and Explorations. Jacquelyn G.Black (2008) 7th Edition;
John Wiley & Sons Pte. Ltd. Asia.
3. Biology of Microorganisms - Brock, TD. Smith D.W. and Madigan M.T. 1984.
4thedition.Englewood, cliffs. N.J. Prentice Hall.
4. Microbiology- Fundamentals and Applications. Ronald M. Atlas. 1989 II edition.
Maxwell Macmillan International Editions.
PEDAGOGY: Chalk and talk, OHP, PPT
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Page No.14
SEMESTER – I
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper II – Microbial Physiology and Metabolism
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about structure and functions of microbial cells,
biomolecule production and its nutritional requirements. The major aim is to make the
students to gain knowledge about physiology of microorganisms and their energy production.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the structure of prokaryotic and eukaryotic cells and transport of molecules. (K2)
CO2: Define the nutrition and growth of microorganisms along with types of culture
techniques. (K1)
CO3: Comprehend the different metabolic pathways, catabolism of biomolecules and
anaerobic respiration. (K2)
CO4: Detailing on important biosynthetic pathways in microorganisms. (K1)
CO5: Describe on pathways and energy generation in photoautotrophs and other
extremophiles. (K5)
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CONTENTS
UNIT-I
Ultra-structure of prokaryotic and Eukaryotic cells: Sub cellular structures – Cell wall,
Cell membrane, Cell envelope, Liposomes. Membrane transport: Diffusion, Active and
Passive transport, group translocation. Iron uptake and sporogenesis.
UNIT-II
Nutrition and growth of microorganisms: Nutritional types of microorganisms, Nutritional
requirements. Factors influencing the growth of microorganisms – Temperature, pH, Pressure
(Osmotic, Hydrostatic), nutrients, moisture and radiations. Physiology of growth – growth
curve and growth measurements. Types of culture techniques: Batch, continuous,
synchronous and Diauxic growth.
UNIT-III
Respiratory metabolism: EMP pathway, HMP Pathway, Entner-Doudoroff pathway,
glyoxalate pathway. Krebs cycle-oxidative and substrate level phosphorylation. Electron
transport system and ATP production. Bioluminescence - Pasteur Effect; Fermentations of
carbohydrates-lactic acid, butyric acid and mixed acid. Lipid catabolism- oxidation
Anaerobic respiration: sulfur compounds, nitrate and CO2 as electron acceptors.
UNIT-IV
Biosynthesis of aspartate, pyruvate, histidine and serine amino acid families – Purine and
Pyrimidine nucleotides, salvage pathway-denovo pathway. Biosynthesis of fatty acids and
phospho lipids. Biosynthesis of Gram positive and Gram negative cell wall.
UNIT-V
Photoautotrophs- Brief account of photosynthetic and accessory pigments-oxygenic-
anoxygenic photosynthesis autotrophic generation of ATP; fixation of CO2 - C3 pathway.
Chemolithotrophic metabolism: Iron, Hydrogen, Nitrogen and sulfur oxidations. General
account of Archaebacteria. Basic physiology of halophiles, thermophiles and methanogens.
TEXT BOOKS:
1. Microbial Physiology. Albert G. Moat & John W. Foster. 1988. 4th ed. John Wiley
and sons.
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REFERENCE BOOKS:
1. Microbial physiology and Metabolism: Caldwell, D.R. 1995. Brown publishers.
2. Chemical Microbiology: An introduction to Microbial physiology: Rose A.
H.1986, Wiley and sons.
3. Bacterial Metabolism - Gottschalk, G .1986, 2ndedition, NY: Springer –Verlag.
4. Bacterial Metabolism - Doelle. H. W. 2005, 2ndEdition.Elsevier publications.
PEDAGOGY: chalk and talk, OHP, PPT
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SEMESTER – I
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper III – Food and Dairy Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge on overview of food related microbes and its interaction with food as
substrate, food spoilage and food-borne illness manifested by food borne microbes, dairy
products and its fermentation.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basic Importance of studying Food and Dairy Microbiology. (K2)
CO2: Evaluate contamination and spoilage of foods and its control through preservation. (K5)
CO3: Exemplify the concepts of food borne diseases and detection methods. (K1)
CO4: Understanding the types of Food Fermentations and Introduction to Probiotics. (K2)
CO5: Explore the concepts of HACCP and its applications in food industry. (K3)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
Food and Dairy Microbiology: Food as a substrate for microorganisms-primary sources of
microorganisms in foods. Factors influencing microbial growth in foods- extrinsic and
intrinsic factors. Microorganisms important in Food Microbiology: Molds, Yeasts and
Bacteria- General Characteristics- Classification and importance.
UNIT-II
Contamination and spoilage: Cereals, vegetables and fruits, meat and meat products, Fish
and sea food - milk, Spoilage of canned foods. Principles of food preservation- Asepsis-
Removal of microorganisms, anaerobic conditions- High temperature- Low temperature -
Drying - Chemical preservatives (Benzoate, sugar and salt).
UNIT-III
Food Borne diseases and microbial analysis in foods: Food borne infections and
intoxications (Bacterial and Fungal) – Botulism - Staphylococcal food poisoning -
Salmnellosis, Shigellosis, Aflatoxin, Patulin and Ochratoxin. Milk borne infection and
intoxication - Indicator organisms in food - Direct examination - microscopic culture
techniques - direct epifluorescent filter technique- enumeration methods - Viable & Total
Count - Dye reduction tests - Chemical methods- ATP bioluminescence and Radiometry-
Immunological methods - ELISA and RIA, Molecular methods – PCR.
UNIT-IV
Food fermentations: Microorganisms as food – Single cell protein – organisms as SCP –
production process– carbon substrates – SYMBA process, PRUTTEN process. Fermented
dairy products - Butter milk, Cream, Yoghurt, Kefir, Koumiss, Acidophilus milk and Cheese
production (Roquefort and Camembert). Food produced by microbes -Bread, Vinegar and
oriental fermented foods (Soy sauce, tempeh and miso). Introduction to Probiotics as foods-
therapeutic and nutritional value (Lactobacillus, Bifidobacterium).
UNIT-V
HACCP in Food Industry: Definition- principles of HACCP –Flow diagram development
of HACCP worksheet and plan form, HACCP audit in food processing plants, HACCP
manual – records for HACCP audit – formats for HACCP records –Applications and
limitations of HACCP.
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TEXT BOOKS:
1. Food Microbiology. Adams MR and MossMO, 2005, 1st Edition, Reprinted,
Published By New Age International (P) Limited, Publishers, New Delhi.
2. Food Microbiology. Frazier WC, Westhoff DC, 1988., TATA McGraw Hill.
REFERENCE BOOKS:
1. Modern Food Microbiology. James M Jay, 2000. 4th Edition, CBS Publishers &
Distributors, New Delhi.
2. Basic Food Microbiology. Banwart GJ, 2004. 2nd Edition, CBS Publishers &
Distributors, New Delhi.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – I
COURSE: M. Sc., Microbiology
PAPER TITLE: PAPER-IV – Molecular Genetics
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVES
To Demonstrate in-depth knowledge of what genes are and how they work; clarify the
mechanisms for the flow of information from genes to proteins, and how these processes are
regulated.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate the molecular machinery of living cells. (K3)
CO2: Describe the process of transcription in Prokaryotes and Eukaryotes. (K1)
CO3: Understand the regulation of gene expression in Prokaryotes and Eukaryotes. (K2)
CO4: Illustrate the mechanism of gene mapping. (K2)
CO5: Describe and identify the mechanism of mutations and its repair. (K1)
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CONTENTS
UNIT-I
Genes and Chromosomes: Mendelian principles, organization of genes, DNA Chemistry &
Structure, Physical and chemical properties of DNA, forms of DNA, DNA Replication
(Prokaryotes and Eukaryotes) - enzymes of replication, semi-conservative model of
replication, theta and rolling circle replication.
UNIT-II
Processing of genetic information: Non-coding RNA – Small, long and circular,
Transcription in prokaryotes and eukaryotes.RNA polymerases (RNAP I, II & III) - inhibitors
of RNAP. Post transcriptional modifications (for all types of RNA) - Capping,
polyadenylation and splicing mechanism, Transcriptional control of gene expression
(promoters, enhancers and CpG islands).
UNIT-III
Gene expression and regulation: Genetic code – Basic Features: Codons, Anticodons,
Universality, Overlapping, Non-overlapping and Redundancy. Translation (initiation,
elongation and termination & factors responsible), post-translational modifications,
Regulation of gene expression (lac and trp operon).
UNIT-IV
Mechanism of genetic exchange: Transformation- competence factors- mechanism of DNA
uptake, transduction- generalized (P1) and specialized (lambda), mapping by co-transduction
frequency, conjugation- F plasmid- F’- Hfr mapping- mapping of unselective recessive
markers, transposons (Tn10 and retrotransposons), genetic recombination.
UNIT-V
Mutation & Genetic disorders: Spontaneous- transition, transversion, missense, non-sense,
and frame shift mutations, Mutagens- physical and chemical- survival curve, mechanism of
repair- light and dark mechanism, SOS. Genetic disorders – β-thalassemia, Sickle cell
anemia, and Down syndrome. Cancer genetics: Loss of cell cycle control, oncogenes, tumor
suppressor genes and apoptosis. Abnormalities in DNA repair mechanisms.
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TEXT BOOKS:
1. Principles of Genetics. Gardner (2001), John Wiley & Sons Inc, New York
2. Principles of Genetics. Robert Tamarin, (1996) 5thEdition.WMC Brown publication,
Boston.
REFERENCE BOOKS:
1. DNA Structure and Function - Richard R. Sinden, (2012), Academic Press.
2. DNA Replication- Arthur Kornberg, 2nd Ed, (2005), University Science Books.
3. Genes IX - Benjamin Lewin, (2007), Jones & Bartlett Learning, New Jersey.
4. Molecular Cell Biology - Harvey Lodish, Arnold Berk, Paul Matsudaira, Chris A. Kaiser,
Monty Krieger, Matthew P. Scott, Lawrence Zipursky, and James Darnell, 8th edition (2016),
Published by W.H freeman and Company, New York.
5. Molecular Biology of the Gene. James D. Watson, Tania A. Baker, Stephen P. Bell&
Alexander Gann, 7th edition (2010), Published by Pearson Education PVT, Ltd. Singapore..
6. Microbial genetics by Stanley R Maloy, (1994), Jones and Bartlett Publishers.
PEDAGOGY: Chalk and talk, OHP, PPT
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Page No.23
SEMESTER – I
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Practical I – Microbial Physiology, Molecular Genetics and Food
Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
The core objectives of the paper is designed for students to study in detail including preparing
stained smears, culturing microorganisms, performing tests to identify bacteria and fungi, and
studying microbial growth control methods
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basic importance of studying Microbiology. (K2)
CO2: Demonstrate fundamental microbiological techniques. (K3)
CO3: Exemplify the concepts of identification and detection methods by biochemical tests. (K1)
CO4: Identify the important pathogens and spoilage microorganisms in foods and the
conditions under which they will grow. (K1)
CO5: Explore the effect of physical mutagen on DNA. (K3)
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Page No.24
CONTENTS
Fundamentals of Microbiology
1. Sterilization – Principles and applications.
2. Media preparation – Liquid and solid media, agar deep, slant and plate.
3. Pure culture technique – Streak plate, pour plate and spread plate.
4. Motility determination – SIM and hanging drop method.
5. Enumeration of Microorganisms – Bacteria, actinomycetes and fungi from soil.
6. Staining techniques – Simple, differential, spore, negative and acid fast staining.
7. Lactophenol cotton blue mounting for identification of fungal morphology.
8. Chromatographic techniques: Paper, TLC.
9. Estimation of sugar by DNSA method.
10. Estimation of protein – Lowry et al., method.
Microbial Physiology
11. Measurement of microbial growth and determination of generation time –
Turbidometric method for bacteria.
12. Anaerobic culture technique – Wright tube method and McIntosh anaerobic jar
method.
13. Biochemical characteristics of microorganisms
Indole production test
Methyl red test
Voges Proskauer test
Citrate utilisation test
Oxidase test
Catalase test
Urease test
Nitrate reduction test
Carbohydrate fermentation test (Glu, Suc, Lac and Mannitol) and TSI test
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Food and Dairy Microbiology
14. Determination of milk quality –MBRT.
15. Isolation and enumeration of microorganisms from spoiled food - (Bacteria & Fungi).
Molecular Genetics
16. Induced Mutagenesis- physical method.
17. Induction of lac operon (ONPG).
REFERENCES:
1. Experiments in Microbiology, Plant pathology & Biotechnology. Aneja, K.R.
2005, 5th Ed NAI Publ.
2. Microbiology, A lab manual, Cappuccino, Sherman, 2008, 6thEdn. Pearson
Education.
3. Microbiology-Practical Lab Manual. Kannan. K, 2002, Palani Paramount Publ.
4. Experimental procedures in Lifesciences. Rajan. S.
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SEMESTER – I
COURSE: M. Sc., Microbiology
SUBJECT TITLE: Paper VIII: Bioethics, Biosafety, TQM and IPR
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVES
To provide basic concepts and importance of bioethics and biosafety, TQM and IPR
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate Ethics and Ethical issues in GMO’s. (K3)
CO2: Describe the Biosafety levels of microbes, plants and animals. (K1)
CO3: Understand the Trade Quality Management. (K2)
CO4: Illustrate the IPR. (K2)
CO5: Describe the concepts of Plant breeding and its rights for protection. (K1)
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Page No.27
CONTENTS
UNIT-I
Bioethics in plant and animal genetic engineering & transgenics - Ethics of genetically
modified microbes, ethics in genetically modified food, ethical issues in human
biotechnology, ethics in stem cell research, gene therapy and biowarfare.
UNIT-II
Biosafety guidelines in India and international level, physical and biological containment,
biosafety levels for microbes, plants and animals, risk assessment and management of
laboratory research, risk assessment of biotechnological products, field trials with genetically
modified plants, planned introduction of genetically engineered microbes.
UNIT-III
Principles of TQM, Tools, steps, techniques and methods for TQM (Six sigma, charts,
Ishikawa diagram, tree diagram, RCA and PDCA cycle), Requirements for supplementing
TQM - steps for supplementing TQM - questionnaire - assessment through questionnaire,
mission statement, benefits of TQM, check list for implementing TQM, Case study.
UNIT-IV
Intellectual property rights- protection of biotechnological inventions, patents- types,
patenting of genes, biological organisms, plants, animals, microbes and transgenic organisms,
trade secrets, copyright, World Intellectual Property Rights organization (WIPO), GATT
(General agreement of tariff and trade), biodiversity bill of India.
UNIT-V
IPR for plant breeding-plant variety protection in India, plant breeder’s rights, farmer’s
rights, protection & implementation of plant varieties, farmer’s rights act, national gene fund,
Benefits of PBR, mechanism involved in terminator technology for seed protection,
technology for pure lines & hybrid seed protection, traitor technology.
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TEXT BOOKS:
1. Intellectual Property Rights, Radhakrishnan R. and Balasubramanian, S. Excel
Books, I ed., 2008.
2. Handbook of Indian Patent Law and Practice, Subbaram N. R.,S. Viswanathan
(Printers and Publishers) Pvt. Ltd., I ed., 1998.
REFERENCE BOOKS:
1. Bioethics and Biosafety in Biotechnology, Sree Krishna, V, New Age International
Publishers, I ed., 2007.
2. Technology Transfer, Goel Cohen, Sage Publications, 2004.
3. Twelve management skills for success- Ram Narain, Viva books private limited,
Chennai.
4. Across functional perspectives of TQM. Rao, Carr, Dambolena and Kopp. John wiley
and sons, Newyork, 1996.
5. Ethics in engineering, Martin. M.W. and Schinzinger.R. 3rd Ed, Tata McGraw-Hill,
New Delhi. 2003.
6. Biosafety issues related to transgenic crops, DBT guidelines, Biotech Consortium
India Limited, New Delhi, 2005.
PEDAGOGY: Chalk and talk, OHP, PPT
160 Director, SBS
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Page No.29
SEMESTER – I
COURSE: M.SC., Microbiology
SUBJECT TITLE: Elective I - Group B: Quality Assessment in Pharmaceuticals
TOTAL TEACHING HOURS/WEEK: 5 HRS
COURSE OBJECTIVE
This paper presents the study of various aspects involved in product quality and quality
assurance, the methodology necessary to establish and implement quality assurance program
for a manufacturing plant with good manufacturing practices.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand different types of antimicrobial drugs and its action. (K2)
CO2: Develop knowledge on microbial spoilage and sterile pharmaceutical products. (K6)
CO3: Comprehend the concepts of sterilization control and methods of sterility testing. (K2)
CO4: Identify criteria for qualified personnel, safety and identification methods along with
biofilms and disinfection agents. (K1)
CO5: Explore the perceptions on validation methods, regulatory requirements and auditing in
pharmaceutical industry. (K3)
MAPPING WITH PROGRAM OUTCOMES
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161 Director, SBS
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Page No.30
CONTENTS
UNIT-I
An introduction to pharmaceutical microbiology. Chemical growth control. Chemical
antimicrobial agents for external use, synthetic antimicrobial drugs, naturally occurring
antimicrobial drugs: Antibiotics. Antibiotics from prokaryotes, antiviral drugs, antifungal
drugs, antimicrobial drug resistance, the search for new antimicrobial drugs.
UNIT-II
Types of spoilage, Factors affecting microbial spoilage – assessment of microbial spoilage –
preservation. Ecology of microorganisms as it affects the pharmaceutical industry – Sterile
pharmaceutical products – injections, Non injectable sterile fluids, ophthalmic preparations,
dressings and implants.
UNIT-III
Sterilization control - methods of sterility testing- sterilization monitors and Quality
assurance of products. The microbiological quality and regulatory requirements for natural
and nutraceutical products, The regulatory control and quality assurance of immunological
products, Containment system integrity – sterile products, Regulatory guidelines
(microbiology) for veterinary antimicrobial products.
UNIT-IV
The role of the Qualified Person in microbiological quality assurance, Safety in
microbiology, Rapid enumeration and identification methods, Selection and use of cleaning
and disinfection agents in pharmaceutical manufacturing, Prevention and elimination of
microbial biofilms in the manufacturing environment using Clean-in-Place, Cleanroom
design, operation and regulatory standards.
UNIT-V
Microbiological quality assurance. Validation of aseptic processing and media fills,
International disinfectant testing protocols, Measurement of biocide effectiveness,
Microbiological quality and regulatory requirements for biotherapeutics and manufactured
products, The role of the microbiologist in HACCP, Auditing the pharmaceutical
microbiology department.
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Page No.31
TEXT BOOKS:
1. Pharmaceutical biotechnology - Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. andRussel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS:
1. Gene therapy - Templeton and Lasic (2000) Marcel and Dekkn.
2. The basic science of gene therapy - Richard C. Mulligan (1993) American
Association for the Advancement of Science.
3. Comprehensive biotechnology - Murray Moo-Young (Ed) Vol-3 (2004) Permagon
Press.
4. Pharmaceutical biotechnology - Purohit (2003) 2nded. Agrobios.
PEDAGOGY: Chalk and talk, OHP, PPT
163 Director, SBS
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Page No.32
SEMESTER – I
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective I- Group C: Basic Biological Sciences
TOTAL TEACHING HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about structure and function of cells, cellular
energetic, protein trafficking and cellular development. Understanding the structural and
functional aspects of the cell provide the student with a strong foundation in the molecular
mechanisms underlying cellular function.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand structure/function of prokaryotic and eukaryotic cells and their intracellular
organelles. (K2)
CO2: Develop knowledge on basic composition and association of various biomolecules. (K6)
CO3: Comprehend the physiology of microbes, animals and plants. (K2)
CO4: Review the origin and evolution of different life forms. (K2)
CO5: Appraise the role of different components of ecosystem in an environment. (K4)
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Page No.33
CONTENTS
UNIT-I
Cell Biology: Structure and function of cells and intracellular organelles (of both prokaryotes
and eukaryotes), mechanism of cell division including (mitosis and meiosis) and cell
differentiation: Cell-cell interaction; Dosage compensation and mechanism of sex
determination.
UNIT-II
Biochemistry: Structure of atoms, molecules and chemical bonds; Principles of physical
chemistry: Thermodynamics, Kinetics, dissociation and association constants; Nucleic acid
structure, genetic code, replication, transcription and translation: Structure, function and
metabolism of carbohydrates, lipids and proteins; Enzymes and coenzyme; Respiration and
photosynthesis.
UNIT-III
Physiology: Response to stress: Active transport across membranes; Plant and animal
hormones: Nutrition (including vitamins); Reproduction in plants, microbes and animals.
UNIT-IV
Evolutionary Biology: Origin of life (including aspects of prebiotic environment and
molecular evolution); Concepts of evolution; Theories of organic evolution; Mechanisms of
speciation; Hardyweinberg genetic equilibrium, genetic polymorphism and selection; Origin
and evolution of economically important microbes, plants and animals.
UNIT-V
Environmental Biology: Concept and dynamics or ecosystem, components, food chain and
energy flow, productivity and biogeochemical cycles; Types of ecosystems, Population
ecology and biological control; Community structure and organisation; Environmental
pollution; Sustainable development; Economic importance of microbes, plants and animals.
TEXT BOOKS:
1. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology – P S Verma &
VK Agarwal., 2004 S. Chand & Sons. Ltd., New Delhi.
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Page No.34
2. Fundamental Processes in Ecology. 2007. Wilkenson DM.
3. Terrestrial Ecosystems - Aber J.D. & Melillo J M. 1991
REFERENCE BOOKS:
1. Economic Zoology. Manju Yadav. Discovery publishing house, New Delhi.
2. Phycology - Lee R E., 1999.
PEDAGOGY: Chalk and talk, OHP, PPT.
166 Director, SBS
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Page No.35
SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper V – Environmental Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a broad knowledge in the field of environmental microbiology such as
generation of waste, different types of pollution, its impact. This paper also discusses the role
of microbes in biological degradation and measures taken to reduce pollution and its effects.
On completion of this paper student will acquire a thorough knowledge of the role of
microorganisms in the environment.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Discuss the kinds of microorganisms in soil and its role in degradation. (K2)
CO2: Explain air pollution, its effects and measures to reduce it. (K5)
CO3: Understand the standards for drinking water, its analysis, types of effluents and water
borne diseases. (K2)
CO4: To classify solid and liquid wastes, its characterization and treatment methods. (K2)
CO5: Understand the microbial degradation of different compounds. (K2)
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CONTENTS
UNIT-I
Microorganism and environment: Microbial population in soil – Bacteria, fungi,
actinomycetes, algae and protozoa – Role of microbes in cellulose, hemicellulose and lignin
degradation. Effect of environmental factors on growth of microbes – Temperature, water,
pH, oxygen and organic matter.
UNIT-II
Air pollution: Sources and major air pollutants – adverse effect on living organisms – acid
rain and its impact on ecosystem– gaseous emissions – Greenhouse Effect – Global warming
– Ozone layer depletion and its effect– Droplet nuclei – Aerosol– Assessment of air quality –
Airborne diseases and preventive measures.
UNIT-III
Water microbiology: Potability of Drinking water – MPN technique. Quality standards for
drinking water. Water pollution – sources and nature of pollutants in water – Sewage –
industrial effluent – Agrochemicals – Eutrophication. Waterborne diseases and preventive
measures.
UNIT-IV
Types of wastes: Types and sources of solid and liquid wastes – Characterization. Solid
waste treatment – Saccharification – Pyrolysis – Composting. Waste water treatment –
Primary treatment: screening, skimming with coagulants, flocculation, and filtration;
Secondary treatment: Aerobic processes – activated sludge, trickling filter, oxidation ponds.
Anaerobic digestion, anaerobic filters, USAB. Tertiary treatment: Activated carbon
treatment, reverse osmosis and electro dialysis.
UNIT-V
Principles and applications of bioaccumulation and biomagnification. Biodegradation:
Microbial degradation of hydrocarbons – alkanes, cycloalkanes, aromatics, polyaromatics
(PAHs). Biodegradation of pesticides – Organo chlorine (DDT) and Organophosphates
(Parathion).
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TEXT BOOKS:
1. Microbial Ecology. Atlas and Bartha,
REFERENCE BOOKS:
1. Environmental Microbiology. 1981. Grant WD, Long PL. Blackie Glasgow and
London.
2. Environmental chemistry. De. A.K. 5th edition. New Age International Publishers.
3. Elements of Environmental Science and Engineering. Meenakshi, P. Prentice Hall.
4. Environmental Biotechnology. Jogdand. P., New Age Publishers.
PEDAGOGY: Chalk and talk, OHP, PPT.
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: PAPER I: Fermentation Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a broad knowledge in the field of Fermentation microbiology and its
processes involved in industries. To provide knowledge in upstream and downstream
processes.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Discuss the industrially important microorganisms and strain improvement. (K2)
CO2: Explain different types of fermenters. (K5)
CO3: Understand the media formulations and types of fermentation. (K2)
CO4: Analyze the methods in downstream process. (K4)
CO5: Understand the commercial production of primary and secondary metabolites. (K2)
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CONTENTS
UNIT-I
General concepts of bioprocess technology: Introduction on upstream processing and
downstream processing - Isolation of industrially important microorganisms - Screening of
microorganisms for industrial products - Strain improvement for increased yield of products -
natural variants, mutation& recombination and Immobilisation of cells- Preservation of
Microbial cultures.
UNIT-II
Fermentors and its functions: Basic component parts-asepsis and containment requirements
temperature control-aeration and agitation systems-mass transfer of oxygen - concepts of
Newtonian and non-newtonian fluids-sterilization of fermenter, air supply and medium;
aseptic inoculation methods-sampling methods - valve systems. Types of fermentor-
Cylindroconical, airlift, deepjet, fluidized bed tower and cyclone.
UNIT-III
Upstream processing: Media formulation - antifoam agents - development of inocula for
bacterial and mycelial fermentation. Types of fermentation - submerged and SSF.
Fermentation systems - Batch, fed batch and continuous. Scale up of fermentation processes -
Application of computer in bioprocess technology.
UNIT-IV
Downstream processing: Recovery and purification of fermentation products - precipitation
methods - filtration devices and filter aids - centrifugation - cell disruption methods - liquid-
liquid extraction - solvent recovery - chromatography - two-phase aqueous extraction -
Super-critical fluid extraction -Ultrafiltration - Crystallization, whole broth processing and
lyophilisation.
UNIT-V
Production of microbial products: Organic acids -Citric acid, Lactic acid and Acetic acid.
Amino acids -Glutamic acid and lysine. Alcoholic beverages - Beer and Wine. Enzymes -
proteases, amylases. Antibiotics -Penicillin.
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TEXT BOOKS
1. Industrial Microbiology: Patel AH 2005. Macmillan India ltd., New Delhi.
2. Industrial Microbiology: Casida, JR., 2005. Published by New Age International
(p) Limited, New Delhi.
REFERENCE BOOKS
1. Industrial Microbiology: Samuel C Presscottand Ceril G Dunn 2005,Agrobios
(India), Agro house, Jodhpur.
2. Microbial Technology: Peppler H.J. and D. Pearlman 2004. 2nd edition, published by
Academic press (An imprint of Elsvier). Volume1 and 2.
3. Principles of Fermentation Technology: Stanbury P.F, A.Whitaker and S.J. Hall
1997, 2nd edition, Pergamum press.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper VII – Recombinant DNA Technology
TOTAL TEACHING HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To provide the students about the basic steps in cloning, the enzymes used and the screening
techniques followed in the Recombinant DNA Technology.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand the enzymes and the prokaryotic vectors which are useful in cloning (K2).
CO2: Understand the use and properties of eukaryotic vectors. Also able to understand the
different methods used for screening of the recombinant clones (K2).
CO3: Understand the sequencing techniques, construction of libraries, and DNA
amplification techniques (K2).
CO4: Understand the use of site directed mutagenesis and the use of DNA in diagnosis of
genetic disorder and infectious disease (K2).
CO5: Understand the methods for creating transgenic plants, animals and microbes (K2).
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CONTENTS
UNIT-I
Gene cloning: Basic steps in cloning, Gene analysis techniques: Isolation of DNA and RNA.
Enzymes used in gene manipulation- Restriction enzymes, Reverse transcriptase, End
modifying enzymes, Methylases, Ligation- DNA ligases, use of linkers & adaptors.
Vectors: Prokaryotic vectors: Plasmid as cloning vectors – Features, pBR322, pUC,
Bacteriophage vectors-λ (insertional and replacement vectors), M13, Cosmids, Phagemids,
Phasmids and BAC.
UNIT-II
Eukaryotic vectors: Yeast Vectors-Yeast integrative vectors, YAC. Animal vectors-SV40,
Retroviral vectors, Plant vectors- Ti Plasmid, plant viral vectors. Specialized vectors-
Expression vectors, Shuttle vectors. Gene Transfer Methods, Selection and Analysis of
recombinant Clones- Direct screening – insertional inactivation and visual screening. Indirect
screening – Nucleic acid blotting (Northern and Southern), Colony, Plaque and Dot blot
hybridization, immunoscreening.
UNIT-III
Construction of Genomic and cDNA Libraries- Strategies for construction of genomic
libraries, Mapping techniques- Chromosome walking and jumping, Restriction mapping-
RFLP, RAPD. DNA sequencing– Maxam and Gilbert method, Sanger’s method. PCR-
Principle, types and Applications.
UNIT-IV
Site directed mutagenesis- Methods, Screening of recombinants for SDM by SSCP and
heteroduplex analysis, DNA foot printing and finger printing, Nucleic acid micro arrays-
Affimetrix®. DNA based diagnosis of genetic disorders- Cystic fibrosis, Diagnosis of
Infectious diseases using DNA- Tuberculosis, Gene therapy- types, strategies and
applications.
UNIT-V
Engineering Genes: Engineering microbes – Insulin production in E. coli, Production of
Vaccines for Hepatitis B Virus using yeast. Engineering plants – Delayed ripening, Pathogen
(insect) resistance. Engineering animals – Tissue plasminogen activator production,
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embryonic stem cells and nuclear transfer methods. Applications of Transgenesis-
Transgenic microbes as biopesticides, Transgenic cattle- increased milk production,
Transgenic fish- improving growth rate, Transgenic sheep- enhanced wool production.
TEXT BOOKS:
1. Gene cloning – An introduction. T. A. Brown, 3rd edition, (1995), Stanley Thomas
publications limited.
2. Principles of Gene Manipulation and Genomics. S.M. Primrose and R.M Twyman,
7th edition, (2006), Blackwell Publishing, Australia.
REFERENCE BOOKS:
1. From Genes to Clones. Ernst. L. Winnacker, 22nd edition, (2003), Panima publishing
Corporation, New Delhi
2. Genes VIII. Benjamin Lewin (2003), Pearson Education Corporation, New Jersy.
3. Essentials of Molecular Biology. Freifelder, George M. Malacinski, 4th Edition,
(2008), Narosa Publishing House, New Delhi.
4. Recombinant DNA technology. J.D. Watson, 2nd edition, (2001), WH Freeman and
Company, New York.
5. Molecular Biotechnology. Glick and Pasternak (1996), Panima Publishing
Corporation, New Delhi.
6. Molecular Cloning: A Laboratory Manual. Sambrook J, Fritsch EF, and Maniatis T
(2000), Coldspring Harbor Laboratory Press, New York.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective I: Group A: Research Methodology and Bioanalytical
Techniques
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge about basics of research methodology, principles and applications of
different instruments, use of statistics in research, understand concepts to handle basic
instruments.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basics of research methodology. (K2)
CO2: Develop knowledge on collection, classification and tabulation of data. (K6)
CO3: Exemplify the concepts of Sampling distribution. (K1)
CO4: Determining views on Microscopy, Spectroscopy and Chromatography. (K4)
CO5: Explore the concepts of different instrumental techniques. (K3)
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CONTENTS
UNIT-I
Introduction to research methodology: Defining the research problem, significance of
research, Stages in research - formulation of hypothesis, objectives of research,
experimental/research design, literature collection, literature citation, investigation, data
collection and analysis, research report, format of thesis, manuscript/research article.
Interpretation and report writing and bibliography.
UNIT-II
Organizing a statistical survey - Planning and execution; Source of data - Primary and
secondary data; Data Collection - methods; Classification and tabulation of data;
Diagrammatic & graphical representation of data. Sampling methods; size of sample.
Measures of central tendency: arithmetic mean, median, mode, quartiles, deciles and
percentiles. Measures of variation: standard deviation. Correlation analysis: Scatter diagram,
Karl Pearson's coefficient of correlation and Spearman's rank method. Regression analysis.
UNIT-III
Sampling distribution and test of significance - Null and alternate hypothesis; Testing of
hypothesis; errors in hypothesis testing; standard error and sampling distribution; sampling of
variables (large samples and small samples). Students’‘t’ test. Chi - square test and goodness
of fit. Analysis of variance: one way and two-way classification, Duncan’s Multiple Range
Test.
UNIT-IV
Methods in Microscopy: Principle and applications of Light, fluorescence, phase contrast,
Confocal, electron microscopy and scanning tunnelling microscopy. Spectrometry and
Spectroscopy: Colorimetry, UV-Visible, Principle of IR and atomic absorption spectroscopy.
Chromatography - Principle and applications: Paper, Thin layer, Ion exchange, Gel filtration,
Affinity, GC, HPLC, GC-MS and LC-MS.
UNIT-V
Centrifugation: Principle and types of centrifugation. Electrophoretic techniques: Agarose,
PAGE, isoelectric focusing and 2D gel electrophoresis, Southern, Northern and Western
blotting techniques. Radiolabeling techniques: An overview of radioisotopes used in biology,
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their detection and measurement; molecular imaging of radioactive material and safety
guidelines.
TEXT BOOKS:
1. Research Methodology, Methods and techniques. C. R. Kothari, New Age
publication
2. Statistical Methods. S.P. Gupta, Sulthan Chand & Sons
3. Principles & Techniques of Practical Biochemistry. Keith Wilson, Kenneth H.
Goulding, Cambridge University Press.
REFERENCE BOOKS:
1. Instrumental methods of chemical analysis. B. K. Sharma, 11th edition, Blackwell
publications.
2. Statistical Methods. Stephen W. Looney (Humana publications).
3. Biostatistics: A Methodology for the Health Sciences. Gerald Van belle, 2nd
Edition, Wiley – Interscience publication.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: PRACTICALS – II (Environmental Microbiology, Fermentation
Microbiology and Recombinant DNA Technology)
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This course provides an overview of environmental and industrial aspects of microbiology
along with nucleic acid methods. It covers the analytical and applied aspects such as
pollutants and its biodegradation along with water quality tests. Students would be aware of
various techniques involving nucleic acids.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Describe the process of quantifying microorganisms from air and water quality tests. (K5)
CO2: Demonstrate the analytical techniques for pollutants and its degradation by microbes. (K3)
CO3: Understand the production process for alcohol, enzymes and its immobilization. (K2)
CO4: Illustrate various nucleic acid methods. (K2)
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Environmental Microbiology
1. Quantification of microorganisms in air – solid impingement techniques.
2. Analysis of Biological oxygen demand.
3. Biodegradation of phenol compounds.
4. Microbial response to heavy metal contaminants.
5. Water quality test-MPN and membrane filtration.
Fermentation Microbiology
6. Wine production and analysis of sugar and alcohol content in wine.
7. Production of extracellular enzyme by submerged fermentation – Protease & amylase.
8. Immobilization of microbes for industrial production of metabolites.
rDNA Technology
9. Isolation of nucleic acid from bacteria.
10. Isolation of plasmids.
11. Restriction digestion and ligation of DNA - Demo
12. Southern blotting for DNA - Demo
13. PCR - Demo
14. Isolation of RNA (Demo).
REFERENCES
1. Experiments in Microbiology, Plant pathology & Biotechnology. Aneja, K.R.
2005, 5th Ed. NAI Publ.
2. Microbiology, A lab manual, Cappuccino, Sherman, 2008, 6thEdn. Pearson
Education.
3. Microbiology-Practical Lab Manual. Kannan. K, 2002, Palani Paramount Publ.
4. Experimental procedures in Lifesciences. Rajan. S.
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective II: Bioinformatics
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge about various tools in bioinformatics, understand basic concepts to
determine the alignment between the sequences and to enable the students to learn the basic
computational skills in bioinformatics-sequence analysis.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basic bioinformatics concepts. (K2)
CO2: Develop knowledge on biological databases. (K6)
CO3: Exemplify the concepts of file format, data storage and retrieval in bioinformatics. (K2)
CO4: Determining views on sequence alignment and how structure can be visualized. (K4)
CO5: Explore the concepts of drug discovery, SNPs and molecular docking studies in
bioinformatics. (K6)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
Introduction to bioinformatics and data generation: Bioinformatics and its relation with
molecular biology. Scope of Bioinformatics, Bioinformatics and Internet, Useful
Bioinformatics sites on WWW, Introduction to single letter code of aminoacids, Symbols
used in nucleotides, Data retrieval- Entrez and SRS. Tools in Bioinformatics (FASTA,
BLAST, BLAT, RASMOL), databases (GENBANK, Pubmed, PDB). Applications of
Bioinformatics.
UNIT-II
Biological Database: Types, introduction of Biological Databases: Nucleic acid databases
(NCBI, DDBJ and EMBL). Protein databases (Primary, Composite and Secondary).
Carbohydrate databases. Specialized Genome databases: (SGD, TIGR and ACeDB).
Structure databases (CATH, SCOP and PDB). Ligand Databases: (Chembank and Ligand
Explorer). Sequence retrieval (Demo).
UNIT-III
Data storage, retrieval, and interoperability: Flat files, relational, object oriented databases
and controlled vocabularies. File Format- (Genbank, DDBJ, FASTA, PDB, SwissProt).
Importance of PERL and PHYTHON (BIOPHYTHON).
UNIT-IV
Sequence Alignments and Visualization: Introduction to Sequences, Alignments and
Dynamic Programming: Local alignment and Global alignment (algorithm and example),
Pairwise alignment - BLAST (Demo) and FASTA Algorithm; Multiple sequence alignment -
Clustal W algorithm (Demo) and Phylogenetics (Building Phylogenetic trees) (Demo). 3D
structure viewers – Rasmol (Demo), SPDBv, Chime, Cn3D and PyMol.
UNIT-V
Introduction to Drug discovery: Introduction: Areas influencing drug discovery, ADME
properties, Molecular Docking, Pharmacogenetics and Pharmacogenomics applications,
Analysis of Single Nucleotide Polymorphisms, an important parameters in drug discovery.
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TEXT BOOKS:
1. Bioinformatics methods and applications: Rastogi.S.C et al., 1993. Prentice Hall of
India Pvt Ltd, NewDelhi, 4th ed., India.
REFERENCE BOOKS:
1. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. Andreas
D. Baxevanis (Editor) and B.F. Francis Ouellete (Editor). Third Edition.
2. Bioinformatics Sequence and Genome Analysis, 2004. David W. Mount, 2nd
Edition.
3. Trends in bioinformatics, 2006. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
4. Principles of Bioinformatics, 2005. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
5. Introduction to Bioinformatics, Attwood A.T and D.J Parry-smith, Pearson
education ltd.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective II-Group B – Communicable and Non-communicable
Diseases
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper deals with the different types of diseases that are communicable and non-
communicable. In addition, this field of science studies various clinical applications of
microbes for the improvement of health and on the aspects of the interrelationship between
infectious agents and their hosts. On successful completion of the subject, the student will
know about the mechanism of infection and its types.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Detailed account on respiratory tract infections. (K1)
CO2: Understandings on intestinal infections. (K2)
CO3: Comprehend the types of vector borne infections. (K2)
CO4: Defining the views on mycological infections. (K1)
CO5: Understand the concepts of non-communicable diseases. (K2)
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CONTENTS
UNIT-I
Respiratory infections – Influenza, Mumps, Measles, Rubella, Acute respiratory infections
and TB.
UNIT-II
Intestinal infections – Polio, Cholera, Acute diarrhea diseases, Food poisoning, Typhoid,
Amoebiasis Ascariasis, Hook worm, Tapeworm, Pinworm infections.
UNIT-III
Vector borne Infections – Leprosy, STD – AIDS – Diagnostic Techniques and Treatment.
UNIT-IV
Superficial mycoses – Dermatophytoses – Opportunistic fungal infections – Candidiasis –
Diagnostics Techniques and Treatment.
UNIT-V
Non-communicable diseases – Hyper Tension – Diabetes – Coronary Heart diseases –
Cancer, Obesity, Blindness, Accidents – Preventive measures.
TEXT BOOKS:
1. Textbook of Microbiology – Ananthanarayanan, R. and JayaramPanicker, C.K.
(2005) 7th edition.Orient Longman Pvt., Ltd.
2. Textbook of Medical parasitology – Subash Chandra Parija, 2nd edition. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, MelnickandAdelberg’s (2001). 22nd edition.
McGraw Hill Medical Publication Division.
2. Medical Microbiology – David Greenwood. Richard Slack and John Peutherer
(2000) 15th ed. ChurchHill Living stone Publication.
3. Medical Parasitology – Chatterjee K.D. (2007). 7th edition.
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4. Medical Microbiology – Patric R. Murray (1990) Mosby Publications.
5. Textbook of Medical parasitology – Jayaram Panicker C.K. (2004) JP Brothers,
Medical publishers, New Delhi.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective II-Group C – Biophysics and Biochemistry
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about the basic biophysical and biochemical
techniques involved in the field of biological sciences. Students after completion of this paper
will be learning on the methods used for the analysis of biomolecules.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Comprehensive account on principles of biophysical methods. (K2)
CO2: Understand principles of nucleic acid techniques. (K2)
CO3: Comprehend the techniques involving radioactive isotopes. (K2)
CO4: Defining the views on metabolism and catabolism of sugars, amino acids, lipids, fatty
acids and sterols. (K1)
CO5: Understand the molecular aspects of cancer. (K2)
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CONTENTS
UNIT-I
Principles of biophysical methods used for analysis of biopolymer structure, X-ray
diffraction, Hydrodynamic methods; plasma emission spectroscopy.
UNIT-II
Principles and techniques of nucleic acid hybridization and Cot curves; Sequencing of
Proteins and nucleic acids; Methods for measuring nucleic acid and protein interactions.
Structural polymorphism of DNA, RNA and three dimensional structure of tRNA.
UNIT-III
Principles and applications of tracer techniques in biology; Radiation dosimetry; Radioactive
isotopes and half-life of isotopes;Effect of radiation on biological system.
UNIT-IV
Interconversion of hexoses and pentoses; Amino acid metabolism; Coordinated control of
metabolism; Oxidation of lipids; Biosynthesis of fatty acids; Triglycerides; Phospholipids;
Sterols, Group transfer and Coupled reactions.
UNIT-V
Biochemistry and molecular biology of cancer; Oncogenes; Chemical carcinogenesis;
Genetic and metabolic disorders; Hormonal imbalances; Drug metabolism and detoxification.
TEXT BOOKS:
1. Introduction to Biophysics - Cortell
2. Molecular and Cellular Biophysics - Meyer B Jackson (2006), Cambridge)
3. Physical Chemistry for Life Sciences - Peter Atkins and Julio de Paula, 2006,
Oxford Press.
4. Biochemistry - Strayer
5. Molecular Cell Biology - Lodish
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REFERENCE BOOKS:
1. Molecular and Cellular Biophysics - Meyer B Jackson (2006), Cambridge)
2. Text Book of Biophysics - R N Roy, New Central Agency (P) Ltd, Culcutta
3. Physical Chemistry for the Biosciences - Raymond Chang,(2004), University book
4. Science Biological Thermo dynamics - Donald, T Hayine, (2007), Cambridge
5. The Cell Molecular Approach - G Cooper and R Hausman (2007) ASM Press
6. Molecular Biology - D Roberties, 8th edition. SAE.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
MODULAR COURSE-MODULE 1
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper I – Introduction to Clinical Laboratory Technology
NUMBER OF HOURS/SEMSETER: 30 Hrs
COURSE OBJECTIVES
To gain knowledge on the clinical assessment of various diseased conditions and routine test
followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: To understand the basics of clinical laboratory. (K2)
CO2: Learn about the preparation of reagents and its storage. (K2)
CO3: Understand the importance of biological samples. (K2)
CO4: To know about the reporting of tests. (K4)
CO5: Disposal of biomedical wastes. (K6)
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CONTENTS
UNIT I : Basic laboratory principles - Code of conduct of medical laboratory personnel.The
use of the laboratory - Basic laboratory principles - Organization of Laboratory Functional
components of clinical laboratories (Cleanliness – Methods of hand wash, Cleaning of lab
instruments, Laboratory floors and table tops, precautions to be taken WRT patients, reports,
analysis). Basic needs of clinical laboratory technician, awareness of soft skills. Safety
measures - Medical laboratory professional and professionalism in laboratory workers - clinic
borne infection and personnel hygiene.
UNIT II: Reagent preparation: preparation of molar, normal, percent solutions. Buffers,
Acid, Base solutions and its storage. Common Laboratory Equipment’s use, principle and
maintenance-spectrophotometer, Incubator, Hot Air Oven, Water Bath Centrifuge, Autoclave
,Microscopy. Glassware – Description of Glassware, its use, handling and care.
UNIT-III: Basic Steps for Specimen collection-types of specimens- Requirement of
Collection - methods of Collection of biological fluids - Anticoagulants - Preservation of
samples - Chemical preservatives . An overview on the transportation of the samples.
UNIT IV: Process of analysing the specimens- labeling the samples. Maintaining the sample
record. Colour code for the samples. Reporting results: a. Basic format of a test report, b.
Release of examination results c. Alteration in reports Quality Management system : Internal
and External quality control. Accuracy, Precision, and Reference value. Levi-Jenning’s chart
(LJ).
UNIT V: Biomedical waste management in a clinical laboratory: Disposal of used samples,
reagents and other biomedical waste - colour coding as per guidelines.
TEXT BOOKS:
1. Medical laboratory technology. Kanai L. Mukherjee, 2007, Vol.1.Tata McGraw
Hill
2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
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REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical laboratory technology Vol.1 Kanai L. Mukherjee, 2007,. Tata McGraw
Hill.
PEDAGOGY: Chalk and Talk, OHP and PPT.
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SEMESTER – III
MODULAR COURSE-MODULE 1
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper II – Techniques in Clinical Laboratory Technology
NUMBER OF HOURS/SEMESTER: 30Hrs
COURSE OBJECTIVES
To gain the knowledge on the clinical assessment of various diseased conditions and routine
test followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1.To understand the procedures of clinical laboratory. (K2)
CO2. Learn about the preparation of reagents and its storage. (K2)
CO3. Understand the importance of biological samples. (K2)
CO4. To know about the histopathogical procedures. (K2)
CO5. To know about blood banking procedures. (K2)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 10 ESE – 40
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CONTENTS
UNIT-I
Basic Hematological diagnosis and Clinical Chemistry: Preparation of Blood Smears-
method (Coverslip, spreader slide and Wedge), Types and causes of poor blood smears (in
diseased condition), Staining Techniques- Leishman’s stain, Wright’s stain, Field’s stain and
Romanowsky stains, Criteria for a good stain: Problem encountered during staining –
Troubleshooting. Total cell count: RBC, WBC and Platelet. Estimation of hemoglobin, PCV
& MCV, MCH, MCHC. Erythrocyte sedimentation Rate (ESR)-Westergen method and its
importance, Differential count, Determination of bleeding time, clotting time and
prothrombin time. Clinical Chemistry: Blood sugar Analysis- glucometer based analysis,
Fasting, Random, Post Prandial, HbA1C, and GTT.
UNIT-II
Collection of Urine - Biohazard management - Routine urine analysis - Colour - Clarity -
Odour - Volume - Chemical Examination - Benedict’s Test - Fehling’s test - Chemistrip
method - Test for Protein in Urine - Heat and Acetic Acid Test - Sulphosalicylic Acid Test -
Heller’s Test. Rotheral’s test, Gerhardt’s test, Fouchet’s Test – Hay’s sulphur test- Test for
Hematuria - Benzidine Test - Guaiacum Test - Gregersen’s Test. Microscopic Examination of
Urine: Crystals, casts and cells. Examination of stool- physical, chemical & microscopic
examination
UNIT-III
Body Fluids: Characteristics of Cerebrospinal Fluid, Synovial fluid, Pleural fluid, Pericardial
fluids, Peritoneal fluids and amniotic fluid. Semen analysis- physical, chemical &
microscopic examination, sperm count and motility.
UNIT-IV
Introduction to Histopathology- Basic steps for Tissue Processing- Fixing, Embedding-
methods- Microtomy- Staining- Mounting, methods of decalcifications –cryopreservation -
Equipments - Microtome -Types, Uses, Parts, different types of microtome knives, care &
maintenance. Automated tissue processor – components-Staining Methods - Hematoxylin &
Eosin stain -Mounting –visualization. Cryostat.
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UNIT-V
Blood banking: Blood Group (ABO & Rh) – methods of grouping & reverse grouping.
Basic blood banking procedures – collection of blood, anticoagulants used, cross matching,
different screening tests including Coomb’s Test (direct and indirect), preparation of different
blood components for use and how to serve a requisition. Preparation of red cell suspension.
Blood transfusion hazards - hemolytic disease of newborn. Apheresis.
TEXT BOOKS:
TEXT BOOKS:
1.Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl
and Edward R. Ashwood, David E. Burns fourth edition, W.B. Saunders Company.
2.Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
3.Handbook of Histopathological and Histochemical technique -
C.F.A.Culling.3rd edition- Butterworths. London.
4.Textbook of MLT, P.B.Godkar. 2nd edition. 2003, Bhalani publication.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical laboratory technology. Vol.1 Kanai L. Mukherjee, 2007,.Tata McGraw Hill
PEDAGOGY: Chalk and Talk, OHP and PPT.
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SEMESTER II
MODULAR COURSE - MODULE II
COURSE: M.Sc., BIOCHEMISTRY
SUBJECT TITLE: PAPER I: HUMAN PHYSIOLOGY
NUMBER OF HOURS/WEEK: 30 HRS
COURSE OBJECTIVE: To provide the students with in-depth knowledge on the functions
of major organs of human body.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL: CO1: To Understand the structure and functions of cells (K2).
CO2: To understand the mystery of complicated function aspects of individual organs in the
body (K2).
CO3: To understand the principles of human physiology (K2).
CO4: To distinguish the cells involved in each system (K4).
CO5: To exemplify the knowledge on the regulation of body functions (K2).
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CONTENTS UNIT I Human Anatomy and Functions- Cell and their functions – Types of muscles and mechanism of muscle contraction – Immune system – Blood components. UNIT II Anatomy and Physiology of Cardiovascular system - Anatomy and Physiology of central nervous system - Anatomy and Physiology of respiratory system. UNIT III Anatomy and physiology of Digestive system, Anatomy and Physiology of excretory system.
CIA – 10 ESE – 40
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UNIT IV Anatomy and Physiology of sensory organs, Anatomy and Physiology of endocrine system. UNIT V Anatomy and Physiology of reproductive system – Menstruation – pregnancy. REFERENCES:
1. Human Physiology - Chaterjee C C , Vol I & II, 11th edition.
2. Review of Medical Physiology - Ganong, , 24th Edition.
3. The Anatomical Basis of Clinical Practice- Gray's Anatomy, 40th Edition.
4. Textbook of Human Anatomy - Ranganathan T S, 5th edition.
5. Text book of Medical Physiology – Sembulingam and PremaSembulingam– 6th
Edition, Jaypee Brothers Medical Publishers (P) Ltd.
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SEMESTER – II COURSE: M. Sc., BIOCHEMISTRY SUBJECT TITLE: MODULE II – PAPER II CLINICAL BIOCHEMISTRY NUMBER OF HOURS: 30 Hrs COURSE OBJECTIVE: Understanding the diagnostic importance of diseases and
disorders.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL: CO 1: To understand the disorders and test associated with various organs (K2). CO 2: To understand the gastric and pancreatic function tests (K2) CO 3: To understand the importance of minerals and acid base balance in human system.
(K2)
CO 4: To assess the abnormalities with respect to various hormones (K5) CO 5: To enable, distinguish and interpret the diseases. (K4)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CONTENTS UNIT I:
An overview of metabolism of carbohydrates, proteins, lipids and nucleic acids – Lipid
profile –Biochemical test for Blood glucose – Fasting, Post prandial, GTT and Hemoglobin
A1C, Renal function test (RFT).
UNIT II:
CIA – 10 ESE – 40
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Gastric, pancreatic function and Liver function (LFT) tests.
UNIT III:
Mineral metabolism - Analysis of Sodium and Potassium electrolytes and acid base balance –
using electrolyte analyser.
UNIT IV:
Biologically important hormones: Insulin, Glucagon, Epinephrine, Thyroid, Growth
Hormone, Parathyroid, Pituitary, Steroid Hormones. Hormone analysis – TFT, pregnancy
test.
UNIT V:
Characteristics of growing tumor cells – biochemical and morphological changes. Cancer
markers – AFP and CEA.
REFERENCES :
1. Tietz Fundamentals of Clinical Chemistry- Teitz, 6th Edition.
2. Practical Clinical Biochemistry -Harold Varley, 2006
3. Introduction to medical laboratory technology – F.J. Baker, R.E. Silverton, 5th
Edition, Butterworth & Co Publishers Ltd.
199 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS) Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
II Year M.Sc., Microbiology
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS)
(2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
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DIVISION OF MICROBIOLOGY
M.Sc., Microbiology
REGULATIONS
MICROBIOLOGY: Activities of microorganisms are very important to almost every sector
of concern to mankind. From a perusal of the foregoing topics, one can find applications
(uses) of microorganisms to agriculture, forestry, food, industry, medicine, and environment.
The scope and significance of microbiology has enlarged manifold, particularly when
importance of environment was realized globally and the word environment was used in a
much wider sense in terms of totality to include almost everything in every bit of nature
OBJECTIVES
After completing the Program of Study in Microbiology, students should be able to:
demonstrate an understanding of core concepts of microbiology, including the
evolution and diversity of microbes; cell structure and function; metabolism;
information flow and the role of microbes in ecosystems.
Display proficiency in basic microbiological skills.
Analyze, interpret and evaluate a range of scientific literature in Microbiology.
Develop an information base for making personal health decisions in regard to
infectious diseases.
ELIGIBILITY
A pass in Under Graduate Examination with any Bioscience Degree or any other examination
accepted as equivalent there by the syndicate B.Sc in Biology / Botany / Zoology/ Chemistry/
Nursing / Biochemistry / Microbiology /or other examinations accepted as equivalent there to
by the Syndicate, subject to such other conditions as may be prescribed therefore.
DURATION OF PG COURSE
The course shall extend over a period of two years comprising of four semesters, with two
semesters per year (six periods – 50 minutes duration each /day). There shall not be less than
ninety instructional days during each semester. Examination shall be conducted at the end of
each semester for the respective subject.
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DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT@ No of Papers Marks
@ Credits
I Subjects and Practicals$ 17 1700 68
II Elective Subjects 4 400 16
III Project 1 150 6
Total 22 2250 90
Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects respectively.
$ In core subjects both theory and practical’s are included wherever applicable
The following parameters are considered throughout study period
i) Regularity of Attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other Social Components (Blood Camp, Fine Arts, etc)
Note: II
The Credit points and Lecture Hours are not linked.
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Page No.4
Annexure No. PMB11 BOS.DT:12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049
SCHOOL OF BIOLOGICAL SCIENCES
M.Sc., MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
Sem
este
r
Par
t
Su
bje
ct C
ode
Subject Title
Inst
. Hrs
per
wee
k
Examination Details
Du
rati
on i
n
Hou
rs.
CIA
En
d S
em
Ex
am
Tot
al M
ark
s
Cre
dit
s
SEMESTER I
I
Paper-I Fundamentals of Microbiology 5 3 25 75 100 4
Paper-II Microbial physiology and
Metabolism 5 3 25 75 100 4
Paper-III Food and Dairy Microbiology 5 3 25 75 100 4
Paper-IV Molecular Genetics 5 3 25 75 100 4
Practical-I
Practicals- I Microbial
physiology, Molecular
genetics & Food
microbiology
5 9 40 60 100 4
Elective-I Elective I 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER II
II
Paper-V Environmental Microbiology 5 3 25 75 100 4
Paper-VI Fermentation Microbiology 5 3 25 75 100 4
Paper-VII Recombinant DNA
Technology 5 3 25 75 100 4
Paper-VIII Bioethics, Biosafety, TQM
and IPR 5 3 25 75 100 4
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Practical -II
Practical II: Environmental
Microbiology, Fermentation
Microbiology and
Recombinant DNA
Technology
5 9 40 60 100 4
Elective-II Elective II 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER III
III
Paper-IX Medical Microbiology 5 3 25 75 100 4
Paper-X Virology 5 3 25 75 100 4
Paper-XI Immunology and
Immunotechnology 5 3 25 75 100 4
Paper-XII Genomics and Proteomics 5 3 25 75 100 4
Practical-III
Practical III: Medical
Microbiology and
Immunology
5 9 40 60 100 4
Elective-III Elective III 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER IV
IV
Paper-XIII Soil and Agricultural
Microbiology 5 3 25 75 100 4
Practical-IV Practical IV: Soil and
Agricultural Microbiology 5 9 40 60 100 4
Elective-IV Elective IV 5 3 25 75 100 4
Project 15 - 100 50 150 6
Total 30 - 190 260 450 18
Grand Total - - - - 2250 90
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Page No.6
Each paper carries an internal component
There is a pass minimum for an external component
Theory: Internal assessment: 25 marks; External component: 75 marks
Practical: Internal assessment: 40 marks; External component: 60 marks
PROJECT AND VIVA VOCE:
Project: 2 Reviews + Dissertation: 150 marks; Final Viva voce: 50 marks
The project report is the bonafide work carried out by the candidate under the
guidance of a faculty authenticated and countersigned by the Director. This project
work must be presented and defended by the candidate in the department attended by
all faculties and reviewed by external examiner. Candidate who has presented the
work as ‘Not qualified as per CBCS’ must resubmit the project again in the ensuing
academic year.
COMPONENTS OF INTERNAL ASSESSMENT*
Theory: Two tests in each semester (one Internal Test and one Model Test). Marks from both
Tests will be taken: Total 15 marks (5 marks Internal Test and 10 marks Model Test)
Assignments and seminar: 2.5 marks + 2.5marks = 5 marks
Overall performance: 05 marks
Practicals I, II, III and IV (for 40 marks):
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Practical: Marks should be awarded to each practical by the course teacher and the average
of the best ten practicals be taken for 40 marks.
Project: 100 marks should be awarded as internal assessment marks (80 marks for two
monthly reviews (40 + 40) conducted by the project guide and the Director, 20 marks for
Dissertation).
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* Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge, subject in charge. The final decision to be made by the
Director.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I / Sem I Research
Methodology
Environment and
Health
Basic biological
sciences
Paper II / SemII Bioinformatics
Communicable and
non-communicable
disease
Biophysics and
Biochemistry
Paper III / Sem III Pharmaceutical
Microbiology
Health care of the
community
Molecular cytology
and tissue
Engineering
Paper IV / Sem IV
Diagnostic
Microbiology and
Molecular
Diagnostics
Nanotechnology Marine Biology and
Aquaculture
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Page No.8
Annex EDC: PG BS BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049.
M. Sc. Biotechnology/Biochemistry/Microbiology/Applied Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2015 onwards)
Note:
The students has to compulsorily select one Extra Disciplinary Course offered by
other departments and are eligible to get 4 extra credits. This paper is offered as self
study from the 2015 batch onwards.
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m
in h
rs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Semester III
Organic farming - 3 25 75 100 4
Total - - - - 100 4
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COURSE: EXTRA DISCIPLINARY COURSE
SUBJECT TITLE: Organic Farming
NUMBER OF HOURS/WEEK: Nil
SUBJECT DESCRIPTION
This course deals with the principles and practices of organic farming for sustainable
development.
GOALS
To make the student to understand basics, importance and advantages of organic farming.
OBJECTIVES
On successful completion of subject student would know about Organic farming,
vermicomposting and their applications.
CONTENTS
UNIT-I
Organic farming- Concept - Principles - Importance- Types- Its relevance to Indian
agriculture - Soil fertility - Nutrient recycling - Crop rotations - Requirements for organic
farming - Advantages and applications of organic farming.
UNIT-II
Methods of organic farming - Land preparation and mulching - Water management-green
manure - Composting methods - Vermicomposting – Different types of earthworms -
Different methods for vermi composting – Vermiwash - Advantages and applications - Value
addition as biofertilizer and marketing strategies.
UNIT-III
Organic farming for sustainable agriculture and crop production - Importance and different
types of biofertilizers - Production of Azolla, Azospirillum, Azotobacter and Rhizobium
biofertilizers- Ecofriendly applications of biofertilizers.
CIA – 25 ESE – 75
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UNIT-IV
Disease and pest management in organic farming - Biological pest control- Bio pesticides -
Neem insecticides - Integrated pest management - Bt insecticides - Advantages and
applications – Control of weeds.
UNIT-V
Integrated farming system - Objectives- Components - Factors affecting ecological balance-
Inspection, Certification and accreditation of organic product - Marketing and export
potential - Organic farming and national economy.
REFERENCES:
1. Hand Book of Organic Farming. Sharma A. 2002. Agrobios.
2. Organic Farming. Lampin N. 1990. Press Books, Lpswitch, UK.
3. Organic Farming and Sustainable Agriculture. Veeresh GK, Shivashankar K &
Suiglachar MA. 1997.
4. Organic Farming – Theory and Practice. Palaniappan SP and Anandurai K. 1999.
Scientific Publ.
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Page No.11
SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper IX: Medical Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
Medical microbiology is a branch of medicine concerned with the prevention, diagnosis and
treatment of infectious diseases. In addition, this field of science studies various clinical
applications of microbes for the improvement of health.
GOALS
To gain knowledge on the aspects of the interrelationship between infectious agents and their
hosts.
OBJECTIVES
On successful completion of the subject, the student will know about the mechanism of
infection and its types & provides the information about the prevention of diseases.
CONTENTS
UNIT-I
Introduction to Medical Microbiology: Role of Microorganisms in medicine, classification
of Medically Important microbes - Normal microflora. Infections: sources and mode of
transmission, infectious disease cycle, epidemic, endemic, pandemic diseases. Antibiotic and
chemotherapeutic agents-Mode of action. Antibiotic resistance – ABST and CLSI guidelines
UNIT-II
Medical Bacteriology: Morphology, cultural characteristics, pathogenicity, laboratory
diagnosis and treatment of: Staphylococcus aureus, Streptococcus pneumonia, Bacillus
anthracis, Corynebacterium diphtheriae, Clostridium tetani, Mycobacterium tuberculosis and
M. lepra, E.coli, Salmonella typhi, Shigella dysentriae, Klebsiella pneumoniae, Proteus
vulgaris, Vibrio cholerae, Pseudomonas aeruginosa and Neisseria gonnorhoea.
CIA – 25 ESE – 75
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UNIT-III
Medical Mycology: Pathogenicity, laboratory diagnosis and treatment of superficial mycoses
(Tinea, Piedra), cutaneous mycoses – Dermatophytoses, Candidosis, Subcutaneous mycoses–
(Sporotrichosis, Mycetoma), Systemic mycosis (Cryptococcosis, Histoplasmosis).
UNIT-IV
Medical parasitology: Morphology and Identification of parasites. Pathogenicity, laboratory
diagnosis and treatment of Protozoan infection of Entamoeba histolytica, Giardia intestinalis,
Trypanosoma cruzi, Plasmodium falciparum, Toxoplasma gondii, Taenia saginata,
Ancylostoma duodenale, Enterobius vemiculari, Ascaris lumbricoides and Wuchereria
bancrofti.
UNIT-V
Systemic Infection: UTI, URTI, LRTI, STD, Skin infections, Nosocomial, PUO– collection
transport and processing of samples.
TEXT BOOKS:
1. Textbook of Microbiology – Ananthanarayanan, R. and JayaramPanicker, C.K.
(2005) 7th ed .Orient Longman Pvt., Ltd.
2. Textbook of Medical parasitology – Subash Chandra Parija, 2nd ed. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, Melnick and Adelberg’s (2001) 22nd ed. McGraw
Hill Medical Publication Division.
2. Medical Microbiology – David Greenwood. Richard Slack and John Peutherer
(2000) 15th ed. Church Hill Living stone Publication.
3. Medical Parasitology – Chatterjee K.D. (2007)7thed.
4. Medical Microbiology – Patric R. Murray. (1990). Mosby Publications.
5. Textbook of Medical parasitology – Jayaram Panicker C.K. (2004). JP Brothers,
Medical publishers, New Delhi.
211 Director, SBS
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Page No.13
SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper X: Virology
NUMBER OF HOURS/WEEK: 5Hrs
SUBJECT DESCRIPTION
This paper presents the study of virus related plant and animals. It provides knowledge about
the epidemiology, pathogenesis and prophylaxsis of DNA and RNA viruses.
GOAL
This paper, virology has been designed to provide the student with a firm foundation in the
aspects of role of viruses in Microbiology, which forms a base for their future research. This
paper also provides the basics about different plant and animal viruses including oncogenic
viruses.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding
about the different forms of viruses related to plant and animals. Isolation, cultivation and
purification of viruses can be understood.
CONTENTS
UNIT-I
Introduction to Virology: History - general properties of viruses-techniques in virology-
cultivation –enumeration – isolation and purification. General introduction to Mycophages,
Phycophages and insect viruses.
UNIT-II
Animal viruses–Classification – structure and replication, epidemiology, pathogenesis,
prophylaxis of DNA viruses – Pox virus, Herpes virus, Adeno virus and Hepatitis virus.
CIA – 25 ESE – 75
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UNIT-III
Animal viruses– Classification – structure and replication, epidemiology, pathogensis,
prophylaxis of RNA viruses –Picorna virus, Orthomyxo and Paramyxo, Polio, Rabies, HIV
Togaand Rota virus and other oncogenic viruses.
UNIT-IV
Plant viruses: Classification – Structure –mode of transmission, symptoms, prevention and
control of RNA viruses – TMV. Double stranded DNA virus- CaMV. Single stranded DNA
virus Geminiviruses. Satellites viruses and viroids.
UNIT-V
Bacteriophages - Classification – single stranded DNA containing phages – structure and
biology of phage 174 and M13. Double stranded DNA containing phages - structure and
biology of T4 and λ. Viral vaccines, interferons and antiviral drugs.
TEXT BOOKS
1. Virology – Lewy, JA, Fraenled, HC. and Owens, RA. (1994) 3rd ed. Prentice Hall.
2. Virology – Saravanan, P (2009) M.J.P. Publishers.
REFERENCE BOOKS
1. Textbook of Microbiology – Ananthanarayanan, R and JayaramPanicker, CK. (2005)
7th ed. Orient Longman, Pvt. Ltd.
2. Introduction to modern virology - Dimmok and Primrose, SB. (1994) 4thed.
Blackwell Scientific Company Publications.
3. An Introduction to viruses – Biswass S.B. and Amita Biswass (1984) 3rd ed. Vikas
Publishing House Pvt. Ltd.
4. Medical Microbiology – Jawetz, E and Melnic, JL. (2001), Tata McGraw Publishing
House, New Delhi.
5. Diagnostic Microbiology – Baron E.J, Peterson, L.R. and Finegold, S.M. (1994)
Bailey N. Scotts, 9th Ed. Mosby Publications.
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Page No.15
SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper XI-Immunology and Immunotechnology
NUMBER OF HOURS/WEEK: 5 hours
SUBJECT DESCRIPTION
This subject describes the defense mechanisms of the higher vertebrates against invading
pathogens, tumor immunology and autoimmunity.
GOALS
To understand the basics of human defense mechanisms against the invading foreign bodies.
OBJECTIVES
On successful completion of the subject, the student will be aware of immune system,
immunity and basic immunological techniques and their applications.
CONTENTS
UNIT-I
Introduction to Immunology, History and scope of Immunology – Cells of immune system –
Hematopoiesis and differentiation – Structure and functions of Lymphocytes – T cells (TH
cells, TC cells, TS cells), B cells and NK cells, Monocytes and Macrophages, Neutrophils,
Eosinophils, Basophils and Mast cells, Organization and structure of lymphoid organs –
Primary lymphoid organs – Bone marrow, Bursa of Fabricius and Thymus. Secondary
lymphoid tissues – Spleen, lymph nodes, MALT, BALT & GALT.
UNIT-II
Primary and Secondary immune response. Immunity – Innate immunity – Different barriers
involved in innate immunity – Acquired immunity – Humoral and cell mediated Immunity.
Antigens – Factors influencing immunogenicity, Adjuvants, Epitopes, Haptens, Super
antigens and mitogens. Antibodies – Structure and functions of different immunoglobulin
classes, Production and applications of monoclonal and polyclonal antibodies. Definition and
CIA – 25 ESE – 75
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Classification of Exo and Endo toxins. Vaccines: Active and Passive immunization, Types of
vaccines: Killed, attenuated and recombinant vaccines.
UNIT-III
Major Histocompatibility Complex (MHC): types – Class I, II and III – distribution and
function – Antigen recognition and presentation – Activation of B and T Lymphocytes –
Cytokines and their role in immune recognition – Structure, types and biology of
complement system – Hypersensitivity reactions – Type I, II, III and IV. Autoimmune
disorders – Organ specific autoimmune diseases – Hashimoto’s thyroiditis & Systemic
autoimmune diseases – SLE and AIDS.
UNIT-IV
Tumor immunology – tumor antigens – immune response to tumors – Cancer
immunotherapy. Transplantation immunology (Graft vs Host reaction (GVHR), Host vs Graft
reaction (HVGR), MLR, HLA typing – Organ transplantation (Bone marrow transplantation,
Kidney transplantation, heart and liver transplantation) – Immunosuppressants and
immunosuppressive therapy.
UNIT-V
Immunotechnology: Salient features of antigen – antibody interactions, In vitro tests –
Precipitation reactions: Precipitation reaction in fluids, Precipitation reaction in gels: Radial,
immunodiffusion, Double immunodiffusion, Immunoelectrophoresis. Agglutination
reactions: Haemagglutination, Bacterial and Passive agglutination. ABO blood grouping,
Coomb’s test, Widal, VDRL, ASO, CRP, ELISA and complement fixation test.
Immunofluorescent techniques – FISH, FACS and immuno electron microscopy.
Immunohistochemistry – An Overview.
TEXT BOOKS:
1. Kuby Immunology. Thomas J. Kindt, Richard A. Goldsby, Barbara A. Osborne
(2007) 6th edition, W.H. Freeman & Company, New York.
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REFERENCE BOOKS:
1. Essentials of Immunology. Ivan Riot (1988), 6th edition, Blackwell Scientific
Publications, Oxford.
2. Fundamentals of Immunology. Paul W.E. (1988) Raven Press, New York
3. Immunology – Jan Klein and Vaclav Horejsi (1999) Wiley-Blackwell, New York
4. Janeway’s Immunobiology – Kenneth Murphy and Casey Weaver – 9th Edison,
Garland Science, New York.
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SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper XII: Genomics and Proteomics
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper deals with genome map, comparative genomics, structural genomics, functional
genomics, protein structure prediction, function and various tools for analysis of proteins.
GOALS
To make the students familiar with genome map, comparative genomics, structural and
functional genomics and proteomics-extensively used in drug discovery and in learning
various tools for analysis of proteins.
OBJECTIVES
To understand the genome architecture and extracting information like gene function, gene
regulation, protein evolution and targets for drug designing.
CONTENTS
UNIT-I
Genomics: Introduction to Genomics, Genome topology: Chromatin, super coiling and
packaging. Genome organization: Genome organization in prokaryotic and eukaryotic
systems: Operon concept. Genome Sequencing-Shot gun, clone-contings, pyrosequening.
NGS (Next Generation Sequencing).
Genome analysis - Chromosome analysis: Basic strategy for genetic analysis in human:
Linkage mapping, physical mapping, genetic mapping and restriction mapping.
UNIT-II
Annotation of the Genome: Various approaches in gene prediction, ORF prediction, Gene
prediction in prokaryotes, Gene prediction in eukaryotes, Hidden Markov Model, Pattern
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discrimination, Evaluation of gene prediction method and Prediction of promoter sequences.
Applications of Genomics
UNIT-III
Functional Genomics: Gene expression analysis by cDNA micro arrays, SAGE. EST
databases (DBEST, UNIGENE).
Genomic Diversity- General Purpose of Comparative Genomics Database: Cog- Cluster of
Orthologous Groups, Kegg Kyoto Encyclopedia of Genes and Genomes, MBGD Microbial
Genome Database, Tools for Genomic Comparison and functions of functional genomics.
UNIT-IV
Principles of Protein classification: Based on Structural features, Phylogenetic relationship,
CATH - Classification by Class, Architecture, Topology, Homology, SCOP - Structural
Classification of Protein, FSSP – Fold classification based on structure - structure alignment,
MMDB - Molecular Modeling Database. Secondary structure prediction: Chou – Fasman /
GOR method, Neural network.
UNIT-V
Analytical proteomics: Sample preparation and processing, Proteome analysis techniques:
2D PAGE, Capillary Electrophoresis, Spectroscopy: NMR, MS and MALDI-TOF and its
variants. 3D structural analysis: X-ray crystallography / X-ray diffraction analysis.
Applications of proteomics.
TEXT BOOKS:
1. Bioinformatics - Methods and Applications, Genomics, Proteomics & Drug
discovery –S.C. Rastogi, Namita Mendiratta & Parag Rastogi. Printice Hall of India
private limited, 4th ed., 2013, New Delhi.
2. Principles of Genome analysis and genomics – S.B. Primrose and Twyman (2003)
3rd Edition, Blackwell publishing.
3. Bioinformatics - Sequence and Genome analysis- David W Mount, CBS publishers
and distributors, 2nd ed., 2005.
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4. Bioinformatics - A practical guide to the analysis of genes and proteins- Andreas
D Baxevanis & B F Francis Ouellette , A John Wiley and Sons, INC., Publications,
2nd ed., 2002.
5. Genomics – The science and technology behind the human genome project –
Charles R. Cantor, 4th ed., 1999.
6. Proteomics. S.R. Pennigton and M.J. Dunn. 2002. 2nd., Viva Books Private Limited.
New Delhi (for Units III and IV and V.)
REFERENCE BOOKS:
1. Proteomics- From protein sequence to function - S R Pennington & M J Dunn
(2002), 2nd ed., Viva Books Pvt. Ltd.
2. Genes VII - Lewin B (2000),7th ed., Oxford University Press, UK.
3. Essential Bioinformatics - Jin Xiong (2006), Cambridge University Press.
4. Recent advances in Bioinformatics - Irfan A Khan & Atiya Khunum (2003), Ukaaz
Publications.
5. Introduction to Proteomics – Principles and applications by Nawin Mishra, 2010.
6. Proteomics in practice – Reiner Westermeier and Tom Naven, 2002.
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SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective III: Group: A- Pharmaceutical Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of enzyme and antimicrobial products production related to
pharmacy. The information about gene therapy and diagnostic aids indicates the usage of
advancement in pharmacy research. Concepts of intellectual property rights and international
trade in the syllabus infer importance of patenting and trading of pharmaceutical products.
GOALS
This paper in microbiology has been designed to provide the student with a firm foundation
in the pharmaceutical aspects of microbiology, which forms a base for their future research.
This paper also provides the basics in IPR for better understanding of industrialization of the
pharmaceutical products.
OBJECTIVES
On the successful completion of the course, the students will get an overall understanding of
production aspects of pharmaceutical important enzymes and products, diagnostic aids and
intellectual property and international trade.
CONTENTS
UNIT-I
Introduction to Pharmaceutical microbiology - The ecology of microorganisms affecting
pharmaceutical industry - The atmosphere, water, skin & respiratory flora of personnel, raw-
materials, packing, equipments, building, utensils etc. Types of microorganisms occurring in
pharmaceutical products. Types of antimicrobial agents and their uses (alcohol, phenolic
compounds, formaldehyde).
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UNIT-II
Antimicrobial agents: Types of Antibacterial, antifungal and antiviral compounds.
Commercial production and mode of action - penicillin, cephalosporin.
UNIT-III
Assay for growth inhibiting substances - Assay for non-medicinal antimicrobials (Phenol
coefficient / Rideal-Walker Coefficient). Drug sensitivity testing methods and their
importance. Assay for antibiotics - Determination of MIC, the liquid tube assay, solid agar
tube assay, agar plate assay (disc diffusion, agar well and cylinders cup method).
UNIT-IV
Gene therapy and diagnostic aids: Disease prevention by vaccines (DNA vaccines), disease
diagnosis probes, monoclonal antibodies, disease treatment products from recombinant
organisms, interferon, antisense nucleotides as therapeutic agents, drug delivery (Viral
delivery and therapeutic strategies, non-viral delivery, gene delivery to skin, use of liposomes
as drug delivery system).
UNIT-V
Intellectual Property: Concepts regarding Intellectual Property (IP) Intellectual Property
Protection (IPP) and Intellectual Property rights (IPR). Economic Importance mechanisms for
protection of Intellectual Property - patents, copy rights, trademarks, Factors affecting choice
of IP Protection penalties for violation, Role of IP in Pharma industry: Global ramifications
and financial implications.
TEXT BOOKS:
1. Pharmaceutical biotechnology – Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. And Russel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS:
1. Gene therapy – Templeton and Lasic. (2000). Marcel and Dekkn.
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2. The basic science of gene therapy – Richard C. Mulligan. (1993). American
Association for the Advancement of Science.
3. Comprehensive biotechnology – Murray Moo-Young (Ed) Vol-3 (2004) Permagon
Press.
4. Pharmaceutical biotechnology – Purohit. (2003). 2nd ed. Agrobios.
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SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective III: Group B – Health care of the community
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
The paper describes about the contact between the individual and the health system.
GOALS
To gain knowledge about the types of health care system provided in the society.
OBJECTIVES
On successful completion of the subject, the student will know about the personal health care,
physical and mental health.
CONTENTS
UNIT-I
Nutrition and health - Balanced Diet, food surveillance, food fortifications- addition of
vitamins and minerals - Adulteration and preventive steps.
UNIT-II
Physical health-care of skin, hair, teeth, eyes, ears, hands and feet-physical exercises and their
importance - Walking and jogging – Yoga and meditation – stress Relief.
UNIT-III
Health Programmes and health education – Malaria control – TB control – AIDS control
programmes and Immunization programmes.
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UNIT-IV
Social sciences and mental health - Sociology; Social structure, culture and customs - social
problems-Mental health - cases of mental illness Alcoholism and drug dependence -
prevention Rehabilitation.
UNIT-V
Family planning, Maternal and child health - Antenatal and Postnatal care - Reproductive and
child Health programme (RCH).
REFERENCES
1. Park’s Text books of preventive and social medicine
2. Immune – biotechnology by Naha & Narain
3. Immunology by Dulsy Fatima & N.Arumugam
4. Food and Nutrition by L.Swaminathan
5. Dietics by Srilakshmi
6. Practice of fertility control and Comprehensive manual. 6th edition by
S.K.Choudhary
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SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective III: Group C – Molecular Cytology and Tissue
Engineering.
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
Molecular Cytology and tissue engineering deals with the study of the structure and functions
of the cells of animals and plants; especially, the study of the cells of the human body, and of
the significance of changes.
GOAL
To provide the knowledge of tissue engineering in the use of a combination
of cells, engineering and materials methods, and suitable biochemical and physicochemical
factors to improve or replace biological functions.
OBJECTIVE
Understanding the principles of basic cell molecular biology and the fundamental and
quantitative principles of tissue engineering.
CONTENTS
UNIT-I
Molecular basis of signal transduction in bacteria, plants and animals; Model membranes;
proteinsorting, secretory and endocytic pathways, cell cycle; Dosage compensation and sex
determination and sex-linked inheritance.
UNIT-II
The law of DNA constancy and C-value paradox; Numerical, and structural changes in
chromosomes; Molecular basis of spontaneous and induced mutations and their role in
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evolution; polypoidy; Environmental mutagenesis and toxicity testing; Population genetics.
Environmental regulation of gene expression.
UNIT-III
Cell and tissue culture in plants and animals; Primary culture; Cell line; Cell clones; Callus
cultures; Somaclonal variation; Micropropagation; Somatic embryogenesis; Haploidy;
Protoplast fusion and somatic hybridization; Cybrids.
UNIT-IV
Genetransfer methods in plants and in animals; Transgenic biology; Allopheny; Artificial
seeds; Gene targeting. Applications of genetic engineering in agriculture, health and industry.
UNIT-V
Histology –Basics of mammalian systems, nutrition, digestion and absorption; Circulation
(open and closed circular, lymphatic systems, blood composition and function); Excretion
and osmoregulation: Homeostatis (neural and hormonal); Bioluminiscence.
TEXT BOOKS:
1. Molecular Biology of the Cell. Alberts, B. et al., (2008). 5th Ed. Garland Publishing
House.
2. The World of the Cell. Becker. (2009). 7th ed. Benjamin-Cummings.
3. Genomes 2. Brown, T. A. (2002). Wilely-Liss.
REFERENCE BOOKS:
1. The Cell. Cooper, G. M. (2004). 3rd ed. ASM Press.
2. Introduction to genetic analysis. Griffiths, A. J. F., Wessler, S. R., Lewontin, R. C.
& Carroll, S. B. 2008. 9th ed. W. H. Freeman and Company, New York.
3. Modern Genetic Analysis: Integrating Genes and Genomics. Griffiths, A. J. F.
(2002). 2nd ed. W. H. Freeman and Company, New York.
4. Tissue Engineering. Clemens Van Blitterswick (2008). Academic Press 1st edition
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SEMESTER – III
COURSE: M.Sc., Microbiology
SUBJECT TITLE: PRACTICAL III: Medical Microbiology and Immunology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This Practical paper deals with the pathogenic microorganism of human beings, serology and
immunological techniques.
GOAL
This paper gives strong basic knowledge in sample collection and identification of various
pathogenic microorganisms.
OBJECTIVE
On the successful completion of the course, the students will get an overall knowledge in
medical microbiology.
CONTENTS
Medical Microbiology
1. Collection, transport and processing of specimens – Urine, sputum, pus, blood, throat
swab, stools and wound exudates.
2. Isolation and identification of specimen associated bacterial pathogens – Escherichia
coli, Klebsiella Sp., Proteus Sp., Salmonella Sp., Shigella Sp., Pseudomonas Sp.,
Staphylococcus Sp., Streptococcus Sp.
3. Antibiotic sensitivity pattern of clinical isolates.
4. Cultivation of animal virus in embryonated eggs (Demonstration)
Serology
A. Agglutination Reactions
5. ABO blood grouping
CIA – 40 ESE – 60
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6. Antistreptolysin-O test
7. C-reactive protein test
8. WIDAL test (qualitative and quantitative)
B. Precipitation Reactions
9. Counter-current immunoelectrophoresis
10. Immunoelectrophoresis
11. Ouchterlony’s double immunodiffusion test
12. Radial immunodiffusion test
13. Dot-ELISA test
REFERENCES:
1. Experiments in microbiology plant pathology and biotechnology – Aneja, K.R.
(2003). 4th ed. New age international publ.
2. Microbiology: Laboratory Manual – James G. Cappuccino and Natalie Sherman
(1999). 6th ed. Benjamin/Cummings Science Publishing.
3. Microbiology Manual – Kannan, N, (2000). Palani Paramount Publishing.
4. Microbiology-Laboratory Manual – Sundarajan, M. (2001)
5. Experimental Procedures in Life Sciences – Rajan, S. (2010) Anjana book house.
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SEMESTER – IV
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Paper XIII – S oil and Agricultural Microbiology
NUMBER OF HOURS/WEEK: 5Hrs
SUBJECT DESCRIPTION
This paper presents the study of soil, microbial inhabitants and its association with plant and
other organisms.
GOAL
This paper is designed to give basic knowledge about types and structure of the soil, both
beneficial and harmful microorganisms present in the soil.
OBJECTIVE
After the completion of this paper, the Students will understand the interaction of
microorganisms with the plants and the production of bio fertilizers.
CONTENTS
UNIT- I
Classification of soils: Physical and chemical characteristics of soil. Soil microflora:
bacteria, actinomycetes, algae, fungi, virus, protozoans & nematodes and their significance.
UNIT-II
Microbial interactions with plants: Phyllosphere and Rhizosphere. Microbial interactions
withanimals-insect and rumen. Brief account on microbial interactions - symbiosis -
mutualism - commensalism - competition - amensalism - synergism - parasitism - predation.
UNIT-III
Biogeochemical cycles:Mobilization and immobilization of elements - carbon cycle –
nitrogen cycle, Biological nitrogen fixation - nitrogenase enzyme - nif genes - symbiotic
nitrogen fixation (Rhizobium, Frankia)- non symbiotic nitrogen fixation (Azotobacter,
Azospirillum), VAM - ecto, endo & ectendomycorrhizae, phosphorous and sulphur, iron.
CIA – 25 ESE – 75
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UNIT-IV
Biofertilizers and Biopesticides: Biofertilizers and their importance in crop productivity –
Mass production of Bacterial biofertilizers (Rhizobium, Azospirillum, Azotobacter and
Phosphate solubilizing bacteria) - Algal, cyanobacterial and fungal (mycorrhizae)
biofertilizers - their significance and practice. Biopesticides: Bacterial (Bt pesticides), fungal
(Trichoderma), Viral biopesticides - Baculovirus, NPV insecticides. Biomass production -
formulation - large scale application.
UNIT-V
Plant pathology: Symptoms, disease cycle and control measures of Bacterial diseases -
Blight of rice, citrus canker, wilt of potato, fungal diseases - Blast of rice, rust of wheat, smut
of sugarcane, red rot of sugarcane and viral diseases - Vein clearing disease, TMV.
Transmission of Viral infections through vectors - grasshopper and aphids.
TEXT BOOKS:
1. Microbial Ecology. Atlas RN and Bartha R. 1998. 4th ed. Benjamin Cummings.
2. Environmental Microbiology. Vijayaramesh. 2008.
3. Soil Microbiology. SubbaRao NS. 2004. 4th ed. Oxford & IBH Publishing Co. Pvt.
Ltd., New Delhi.
REFERENCE BOOKS:
1. Soil Microbiology - Mishra RR 2004. 1st edition, CBS Publishers and distributor
New Delhi.
2. Agricultural Microbiology. Rangaswami G and D.G. Bagyaraj, 2002. 2nd ed.
Prentice Hall of India, New Delhi.
3. Biofertilizers in Agriculture and Forestry, Subbarao N.S. 1995. 3rd ed. Oxford and
IBH publishing Co. Pvt. Ltd, New Delhi.
4. Microbial Ecology – Atlas R.N. and Bartha R. 1998. 4th ed. Benjamin Cummings.
5. Soil Microbiology – Robert L Tate. 1995. 1st ed. John Wiley and Sons., Inc. Newyork.
6. Introduction to Soil Microbiology. Alexander M. 1977. John Wiley & Sons., Inc.
New York.
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SEMESTER – IV
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective IV: Group: A – Diagnostic Microbiology and Molecular
Diagnostics
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of diagnosis of various infectious agents.
GOALS
This paper has been designed to understand the basics of medically important
microorganisms and to diagnosis of a pathogen through safe handling and processing
techniques
OBJECTIVES
On the successful completion of the course, the students will have an overall understanding
of various techniques required to isolate the given pathogen.
CONTENTS
UNIT-I
General concepts in specimen collection and handling in bacterial infections: Basic principles
of specimen Collection – Preservation, storage, transport of specimen (urine, stool, pus and
blood) and processing of clinical samples – Direct examination – Microscopy – Culture
methods for bacteria.
UNIT-II
Emerging technologies in detection of bacterial infections: Microscopic methods for rapid
detection – (Vitek, Microscan, Sensititre and Pasco methods). Direct microbial antigenic
detection – Precipitation test (Immunodiffusion test), agglutination test (Latex),
Immunofluorescence assay, Enzyme immunoassay (ELISA). Serological diagnosis –
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complement fixation. Neutralization test (ASO test), Western blot technique. Molecular
Methods – Nucleic acid hybridization and PCR.
UNIT-III
Lab diagnosis of viral infections: Specimen selection and collection – throat, naspharyngeal
swabs, bronchial washes, rectal swabs, stool and skin lesions. Virus detection methods –
cytology and histology, Electron microscopy, Immunodiagnosis – antigen detection, PCR and
cell culture (cell line and cytopathic effect).
UNIT-IV
Lab diagnosis of fungal infections: collection and transport of clinical specimens –
respiratory tract secretions, CSF, blood and urine. Identification methods – direct detection,
microscopy and cultivation of dermatophytes. Opportunistic mycoses – Aspergillus spp,
Systemic mycoses – Histoplasma capsulatum and Candida albicans
UNIT-V
Lab diagnosis of parasitic infections: General specimen collection and transport of specimens
- Approaches in identification - O&P examination - pinworm examination - Biopsy
examination - blood smear staining (thick and thin). Cultivation - Larval stages, nematodes
and protozoa. Organism identification in body organs - amoeba and flagellates.
TEXT BOOKS:
1. Diagnostic Microbiology (Bailey & Scott’s) - Betty A Forbes and Daniel Sahm, 12th
edition, Mosby Elsevier publications.
2. Textbook of Microbiology– Ananthanarayanan, R. and Jayaram Panicker, C.K.
(2005). 7th ed. Orient Longman Pvt., Ltd.
3. Textbook of Medical parasitology- Subash Chandra Parija, 2nd ed. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, Melnick and Adelberg’s (2001). 22nd ed. McGraw
Hill Medical Publication Division.
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2. Medical Microbiology - David Greenwood. Richard Slack and John Peutherer.
(2000). 15th ed. Church Hill Living stone Publication.
3. Medical Parasitology – Chatterjee K.D. (2007) 7th ed.
4. Medical Microbiology - Patric R. Murray (1990) Mosby Publications.
5. Textbook of Medical parasitology– Jayaram Panicker C.K. (2004). JP Brothers,
Medical publishers, New Delhi.
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SEMESTER – IV
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Elective IV: Group: B – Nanotechnology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
The paper describes about the study of the controlling of matter on an atomic and molecular
scale. Nanotechnology is a find that turned the world around.
GOALS
To gain knowledge about the nanoparticles of various sizes and their biological importance.
OBJECTIVES
On successful completion of the subject, the student will know about the projected ability to
construct items from the bottom up, using techniques and tools being developed today to
make complete, high performance products.
CONTENTS
UNIT-I
Basics of Nanotechnology I: Background to Nanotechnology – scientific revolutions – types
of nanotechnology and nanomachines – atomic structure molecules & phases – molecularand
atomic size – surfaces and dimensional space – top down and bottom up Nanoscale
formation.
UNIT-II
Forces between atoms and molecules: Strong intermolecular forces - covalent and coulomb
interactions – interactions involving polar molecules and polarization - weak intermolecular
forces and total intermolecular pair potentials - Van der Waals forces - repulsive forces;
special interactions such as hydrogen - bonding, hydrophobic and hydrophilic interactions.
CIA – 25 ESE – 75
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UNIT-III
Nanostructures and their properties: Definition of nano systems - dimensionality and size
dependent phenomena in Quantum dots, and Quantum wires - size dependent variation in
magnetic, electronic transport properties.
UNIT-IV
High vacuum technology: Evaporation theory – different sources for evaporation - working
principles of rotary and diffusion pumps – cryogenic pumps – cryo sorption and getter pumps
- vacuum materials.
TEXT BOOKS:
1. Nanotechnology: basic science and emerging technologies – Mick Wilson, Kamali
Kannangara, Geoff Smith, Michelle Simmons, Burkhard Raguse, Overseas Press
(2005)
2. Amorphous and Nanocrystalline Materials: Preparation, Properties, and
Applications, A. Inoue, K.Hashimoto (Eds.,) (2000)
REFERENCE BOOKS:
1. Understanding Nanotechnology. (2002). Scientific American, editors at Scientific
American, Warner Books.
2. Introduction to Nanotechnology. (2003). Charles P. Poole, Frank J. Owens, Wiley-
Interscience.
3. Nanotechnology: A Gentle introduction to the Next Big Idea. (2002). Mark A.
Ratner, Daniel Ratner, Mark Ratne. 1st edition. Prentice Hall PTR
4. Fundamentals of Surface and Thin Film Analysis. Leonard C. Feldman and James
W. Mayer.
5. Hand book of thin film technology. L.I. Maissel and R. Glang. McGraw-Hill Book
Company.
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SEMESTER – IV
COURSE: M.Sc., Microbiology
PAPER TITLE: Elective-IV- Group C – Marine Biology and Aquaculture
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper deals with the aquatic environment and the types of biological community present
in it and adds on the fisheries management and its pathology.
GOAL
The completion of this paper provides the students to get are of the marine environment and to
create basic understanding of the nutritional requirements of fish/shellfish.
OBJECTIVE
To provide a deep knowledge on the marine biology and aquaculture.
CONTENTS
UNIT-I
Biological Oceanography: The sea as a Biological environment: classification of marine
environment; general characters of the populations of the primary biotic divisions (plankton,
nekton and benthos) - Introduction of plankton; general classification and composition of
plankton; floating mechanism of plankton. Collection of plankton; general account of
instruments and nets employed; methods of fixation and preservation of plankton; analysis of
samples, methods of estimating standing crop of plankton.
UNIT-II
Biology of Marine Organisms-I: -Nutrition: Types of food, general mechanisms of feeding;
transport of food through gut: digestion, digestive enzymes- Excretion – Nitrogen excretion;
mode of nitrogen excretion, Elimination of nitrogenous waste - Respiration respiratory
mechanisms: factors effecting respiration (Salinity, temperature and oxygen tension);
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respiratory pigments: role in transport of oxygen and carbon dioxide. Osmotic regulation and
ion regulation: mechanisms: general account.
UNIT-III
Estuaries and Coastal Zone management: Estuarine environment: classification and
physico - chemical parameters of estuaries. Distribution of estuarine plankton, nekton and
benthos. Estuarine birds, estuarine food web. Mangroves, sea-grasses, marine fungi- Coastal
Zone Management: Coastal resources i.e. finish, shellfish and non-living resources and their
management. Impact of dredging, mining and pollution on coastal habitats and their
management methods. Remote sensing application in coastal zone management. Coastal zone
regulations.
UNIT-IV
Basics of fish and shellfish health management: Host-pathogen-environment relationship,
Management of culture systems, Environmental stress. Diseases in aquaculture: parasitic,
bacterial, viral and fungal pathogens of fish and shellfish. Water, soil, environmental
parameters and their effects on fish health. Disease in hatcheries and grow out systems.
Epidemiology of diseases, nutritional pathology.
UNIT-V
Defense system in fish and shellfish: Defense systems in fish, innate and acquired
immunity, inflammation response to diseases. Antibody and cell mediated immunity in fish
and shellfish. Techniques in health management: Microbiological, haematological,
histopathological, immunological and molecular techniques. Disease surveillance and
reporting.
TEXT BOOKS:
1. Marine Biology. Friedrich H., Sidgwick & Jackson, 1969.
2. Ecology of estuaries. Mc Lusky D.S., Hinmann, 1971.
3. Fish diseases. Aline, W. 1980. Spranger – Verlay, Berlin Heidelberg, New York.
237 Director, SBS
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Page No.39
REFERENCE BOOKS:
1. Fish diseases (Vol. I & II). Schaperclaus, W. 1986. Oxonian Press Pvt. Ltd.
2. Environmental Stress and Fish Diseases. Wedmeyer, G. Meyer, F.P. and Smith, L.,
1999. Narendra Publishing House, New Delhi.
3. Fish Pathology. Ronald J., Roberts E. 1989. 2nd Edition, Baillere Tindall, London.
4. Principal diseases of Marine Fish and Shelfish. Vol. I & II. 1990. Carl, J.
Sindermann. 2nd Edition. Academic Press.
238 Director, SBS
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SEMESTER – IV
COURSE: M.Sc., Microbiology
SUBJECT TITLE: Practical IV- Soil and Agricultural Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
CONTENTS
1. Isolation of nitrifiers, denitrifiers and ammonifiers
2. Isolation of microbes from rhizosphere
3. Isolation of microorganism from phyllosphere
4. Isolation of cellulase producers from soil
5. Isolation of phosphate solubilisers from soil
6. Isolation of free living and symbiotic nitrogen fixers
7. Isolation of plant pathogens
(a) Bacteria
(b) Fungi
8. Production of vermicompost from vegetable waste.
9. Production of blue green algae.
10. Production of Rhizobium as biofertilizer.
REFERENCES:
1. Experiments in Microbiology plant pathology and biotechnology – Aneja, KR.
(2003) 4th ed. New age international publ.
2. Microbiology – Laboratory Manual – James G. Cappuccino and Natalie Sherman
(1999) 6th ed. Benjamin/Cummings Science Publishing.
3. Microbiology Manual – Kannan, N, (2000) Palani Paramount Publishing.
4. Microbiology-Laboratory Manual – Sudarajan, M. (2001)
5. Experimental procedures in Lifesciences – Rajan, S. (2010) Anjana book house.
CIA – 40 ESE –60
239 Director, SBS
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SEMESTER – IV
COURSE: M.Sc., Microbiology
PROJECT WORK
(Duration: 1st December to 28th February)
TOTAL PROJECT HOURS/WEEK: 15
Rules and regulations to be followed:
Project can be carried out at the college premises or at any research institution* or
renowned industry* for a period of three months (*prior permission is required from
the Director).
Allotment of guides will be done as per the Lot system in the third semester.
Review of the project work will be conducted in two stages in the month of Jan and
Feb (after internal and model examinations) for Internal and External students.
For Internal Projects:
The concern guides will maintain the attendance. At the end of each month they have
to submit a consolidated attendance to the class in charge.
Requirement of chemicals and glasswares for the project work will be submitted to
the Director with the recommendation from concerned guides on or before 30th of
October. Purchase of expensive items (chemicals or imported chemicals or special
glasswares) will not be permitted.
Students who are doing their project work at CMS shall submit a weekly report of
their research work to their respective guides. Moreover, they have to submit a
monthly report (in MS word format as attachment) to the Director and a copy to be
mailed to cmssbs@gmail.com on or before 30th of each month.
For External Projects:
After getting the permission students can approach the research institute or industry.
Request letter to the Industry or institution can be received from the Director.
CIA – 100 ESE – 50
240 Director, SBS
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Page No.42
After acceptance, a confirmation/acceptance letter or email to be submitted to the
Director through proper channel (ie., signed by the guide and CC).
Students who are doing their project work in external labs shall submit a weekly
report of their research work to their guide by letter or email. Moreover, they have to
submit a monthly report (in MS word format as attachment) to the Director by email
and a copy to cmssbs@gmail.com on or before 30th of each month.
At the time of completion of their project work in the month of February, students
have to submit original attendance certificate (in letter pad) from research lab or
Industry to the Director duly recommended and forwarded by the guides and CCs.
Based on the recommendations by the guide, attendance will be provided to the
students. The original certificate approved by the Director to be enclosed in the thesis.
Dissertation:
A Common format of the dissertation to be followed by all the students of SBS. A
template of dissertation will be given to each student by email in the month of
January.
Final copy will be given for printing after the approval by the concerned guide.
At least one publication of their project work in any journal with ISSN number is
recommended.
Students have to submit three copies of their dissertation duly signed by the guide, CC
and the Director (One original and two copies neatly bound) at the time of viva voce
examination.
Any deviations from above mentioned rules and regulations will not be considered.
***************************************************************************
241 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
I Year M.Sc., Applied Microbiology
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS)
(2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017
242 Director, SBS
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Page No.2
DEPARTMENT OF MICROBIOLOGY
VISION OF THE DEPARTMENT
To produce competent professionals in every field of Microbiology by providing inspirational
learning process to complement our national needs and to be recognized as a Centre of
Excellence in Research, Innovation, Consultancy and Entrepreneurship.
MISSION OF THE DEPARTMENT
To provide facilities and offer opportunities for graduate and post-graduate education in
microbiology through advanced instruction/teaching methodologies, quality training and
innovative research;
To prosecute and promote research in microbiology for the advancement of knowledge
and for the betterment of society.
To serve as a centre for fostering co-operation and exchange of ideas between the
academic, research and industrial sector.
M.Sc., APPLIED MICROBIOLOGY COURSE
PROGRAM EDUCATIONAL OBJECTIVES
PEO 1: To acquire basic knowledge on core concepts in application oriented fields of
Microbiology.
PEO 2: To inculcate practical training on basic techniques and processes in Microbiology.
PEO 3: To impart skill-based education for entrepreneurial development.
PEO 4: To adapt with the continuously evolving technologies in industry.
After completion of the program the graduates will be able to
PO 1: Display proficiency in advanced microbiological skills.
PO 2: Analyze, interpret and evaluate a range of scientific literature in microbiology.
PO 3: Improve analytical and technical skills to enhance employment potential
PO 4: Acquire a holistic professional career in hospitals, food and pharmaceutical industries.
243 Director, SBS
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Page No.3
MAPPING OF PEO’S AND PO’S
PEO1 PEO2 PEO3 PEO4
PO1
PO2
PO3
PO4
DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT@ No of Papers Marks
@ Credits
I Subjects and Practicals$ 17 1700 68
II Elective Subjects 4 400 16
III Project – 100 4
IV Industrial/Institutional Training** – 50 2
Total 22 2250 90
Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects respectively.
$ In core subjects both theory and practical are included wherever applicable.
** Student has to undergo compulsory training for a minimum period of 15 days.
The following parameters are considered throughout study period
i) Regularity of Attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other Social Components (Blood Camp, Fine Arts, etc)
Note: II
The Credit points and Lecture Hours are not linked.
244 Director, SBS
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Page No.4
Annexure: PAMB11 BOS.DT: 12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049
SCHOOL OF BIOLOGICAL SCIENCES
M.Sc., APPLIED MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION - CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2017)
Sem
este
r
Par
t
Su
bje
ct C
ode
Subject Title
Inst
. H
rs p
er
wee
k
Examination Details
Du
rati
on
in H
ours
.
CIA
En
d S
em.
Exa
m
Tot
al
Mar
ks
Cre
dit
s
SEMESTER I
I
Paper-I Fundamentals of Microbiology 5 3 25 75 100 4
Paper-II Microbial physiology and Metabolism
5 3 25 75 100 4
Paper-III Food and Dairy Microbiology 5 3 25 75 100 4
Paper-IV Molecular Genetics 5 3 25 75 100 4
Practical-I Practical-I: Microbial physiology, Molecular genetics and Food microbiology
5 9 40 60 100 4
Elective-I Elective I 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER II
II
Paper -V Microbial Techniques 5 3 25 75 100 4
Paper -VI Industrial Microbiology 5 3 25 75 100 4
Paper-VII Recombinant DNA Technology
5 3 25 75 100 4
Paper-VIII Research Methodology and Bioanalytical Techniques
5 3 25 75 100 4
245 Director, SBS
Anx.PAMB11, 1M.Sc.AMB (CMS College) 2017 2017-19 batch
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Practical-II
Practical II: Microbial Techniques, Industrial Microbiology and Recombinant DNA Technology
5 9 40 60 100 4
Elective-II Elective II 5 3 25 75 100 4
Total 30 - 165 435 600
24
SEMESTER III
III
Paper-IX Medical Microbiology 5 3 25 75 100 4
Paper-X Virology 5 3 25 75 100 4
Paper-XI Immunology and Immunotechnology
5 3 25 75 100 4
Paper-XII Genomics and Proteomics 5 3 25 75 100 4
Elective III Elective III 5 3 25 75 100 4
Practical-III Practical III: Medical Microbiology and Immunology
5 9 40 60 100 4
Industrial / Institutional training.
- - - - 50 2
Total 30 - 165 435 650
26
SEMESTER IV
IV
Paper-XIII Environmental and Agricultural Microbiology
5 3 25 75 100 4
Elective-IV Elective IV 5 3 25 75 100 4
Practical-IV Practical IV: Environmental and Agricultural Microbiology
5 9 40 60 100 4
Project 15 - 60 40 100 4
Total 30 - 150 250 400 16
246 Director, SBS
Anx.PAMB11, 1M.Sc.AMB (CMS College) 2017 2017-19 batch
Page No.6
Grand Total - - - - 2250 90
Each paper carries an internal component
There is a pass minimum for an external component
Theory: Internal assessment: 25 marks; External component: 75 marks
Practical: Internal assessment: 40 marks; External component: 60 marks
* Project: 2 Reviews (20+20) and Attendance (20): 60 marks; Final Viva voce: 40 marks
* The project report is the bonafide work carried out by the candidate under the guidance of a
faculty authenticated and countersigned by the Director. This project work must be presented
and defended by the candidate in the department attended by all faculties and reviewed by
external examiner.
** Industrial / Institutional training:
Each student has to undergo an Industrial / Institutional training during the summer vacation
prior to Semester III. This should be of a minimum duration of 15 working days at any off-
campus site approved by the Director. Students are required to submit three copies of
Industrial / Institutional training report with attendance certificate upon completion and the
bonafide report carried out by the candidate under the guidance of a faculty authenticated and
countersigned by the Director. A report on the Industrial / Institutional training must be
presented by the candidate in the Department (at the end of third semester) attended by all
faculty and reviewed by the respective guide as examiner.
@ Industrial / Institutional training :
Attendance and internal assessment by the guide = 30 marks (Internal)
Final Viva Voce = 20 marks (External) (Dissertation = 10 marks + Viva = 10 marks)
COMPONENTS OF INTERNAL ASSESSMENT*
Theory: Two tests in each semester (one Internal Test and one Model Test). Marks from both
Tests will be taken: Total 15 marks (5 marks Internal Test and 10 marks Model Test)
247 Director, SBS
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Page No.7
Assignments and seminar: 2.5 marks +2.5marks = 5 marks
Overall performance: 5 marks
Practical I, II, III and IV (for 40 marks):
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 5 marks
Practical: Marks should be awarded to each practical by the course teacher and the average of
the best ten practicals be taken for 40 marks.
Project: 60 marks should be awarded as internal assessment marks (40 marks for two
monthly reviews conducted by the project guide, course coordinator and the Director, 20
marks for attendance).
* Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge, subject in charge and course coordinator. The final
decision to be made by the Director with the approval from the Principal.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I/Sem I Bioethics, Biosafety,
TQM and IPR Quality assessment in
pharmaceuticals Basic biological
sciences
Paper II/ Sem II Bioinformatics Communicable and non-communicable disease
Biophysics and Biochemistry
Paper III/Sem III
Pharmaceutical Microbiology
Health care of the community
Molecular cytology and tissue
Engineering
Paper IV/Sem IV
Diagnostic Microbiology and
Molecular Diagnostics
Good Manufacturing/Laboratory
Practices
Computational Molecular Biology
248 Director, SBS
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Page No.8
Annex EDC: PG AMB BOS DT: 12:06:2017
CMS COLLEGE OF SCIENCE AND COMMERCE, (AUTONOMOUS)
COIMBATORE – 641 049.
M. Sc. Biotechnology/Biochemistry/Microbiology/Applied Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2015 onwards)
Note:
The student has to compulsorily select one Extra Disciplinary Course offered by other
departments and is eligible to get 4 extra credits. This paper is offered as self study from
the 2015 batch onwards.
Par
t
Su
b c
od
e
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m
in h
rs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
SEMESTER III
Organic farming - 3 25 75 100 4
Total - - - - 100 4
249 Director, SBS
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Page No.9
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641 049.
M.Sc., MICROBIOLOGY
MODULAR COURSE
Post-Graduate Diploma in Clinical Laboratory Technology (PG Dip. in CLT)
(For the students admitted during the academic year 2017 onwards)
(*SS – Self study paper, T – Taught programme).
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ se
m
Examinations
Du
r.
Exa
m
in
hrs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Module I – Paper I – Introduction
to Clinical Laboratory
Technology (T*) 30 1.5 10 40 50 2
Module I – Paper II – Techniques
in Clinical Laboratory
Technology (T*) 30 1.5 10 40 50 2
Module II – Paper I - Human
Physiology (SS*) 30 1.5 10 40 50 2
Module II – Paper II – Clinical
Biochemistry (T*) 30 1.5 10 40 50 2
Module II – Paper III – Clinical
Microbiology (T*) 30 1.5 10 40 50 2
Module III – Paper I –
Endocrinology and Tumour
markers (T*) 30 1.5 10 40 50 2
Module III – Paper II –
Immunology, Serology and
Parasitology (SS*) 30 1.5 10 40 50 2
Module III– Paper III – Molecular
Diagnostics (SS*) 30 1.5 10 40 50 2
Total 240 - - 400 16
250 Director, SBS
Anx.PAMB11, 1M.Sc.AMB (CMS College) 2017 2017-19 batch
Page No.10
Note:
The student has to undergo compulsorily a PG Diploma course offered by the
department and the student is eligible to get 2 extra credits for each paper. This PG
Diploma course consists of module 1, 2 and 3. Module 1 consists of 2 papers, Module
2 consists of 3 papers and Module 3 consists of 3 papers. Total credits of the modular
course are 16. After the successful completion of the Module I, II and III, students are
eligible to get a PG Diploma in Clinical Laboratory Technology
Minimum 20 days of clinical laboratory training is compulsory during Ist and IInd year.
Year Odd semester Even semester Module details
I 1st paper, 2nd paper
(Module 1) - teaching
1st paper (Module 2) –
Self Study
2nd paper (module 2) –
Teaching
Module 1 & 2
II 3rd paper (Module 2) –
teaching
1 paper (Module 3) –
teaching
2 paper (Module 3) –
Self Study
3 paper (Module 3) –
Self Study
Module 2 & 3
1. Upon completion of 60 hours in module – 1, the students are eligible to receive
“Certificate in Clinical Laboratory Technology” along with four extra credits.
2. Upon completion of 150 hours inclusive of module – 1 & 2, the students are eligible to
receive “Diploma in Clinical Laboratory Technology” along with two extra credits.
3. The PG students those who complete the module – 1 & 2 along with module – 3 with a
total of 90 hours are eligible to receive “Post-Graduate Diploma in Clinical
Laboratory Technology”.
4. The PG students those who have already completed the Module – 1 & 2 in their UG
stream can directly appear for module – 3 and upon completion are eligible to receive
“Post-Graduate Diploma in Clinical Laboratory Technology”.
5. Students will be eligible for credits only upon completion of entire module.
251 Director, SBS
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Page No.11
6. Instructional hours for each paper shall comprise 20 contact hours, 2 hours for end
semester examination and 8 hours of activity which may include any of the following;
Case study/field visit/lab hours/industrial training.
7. For UG certificate and Diploma programme all the papers will have contact classes.
For PG Diploma, 1 paper will be contact and other 2 papers will be self-study.
252 Director, SBS
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Page No.12
SEMESTER – I
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: PAPER I: Fundamentals of Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
The paper will provide a basic understanding of microbes, their biology, and their importance
and describe basic concepts of microbiology. Impact/role of microorganism on life and
provide a foundation for later study in biological sciences.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Recall the important events occurred in the field of Microbiology. (K1)
CO2: Assess the phylogenetic relationship among the Microorganisms. (K5)
CO3: Understand the sterilization techniques and staining methods. (K2)
CO4: Describe the classification of Algae and Fungi. (K5)
CO5: Describe the Exploitation of Microbes. (K5)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 25
ESE – 75
253 Director, SBS
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Page No.13
CONTENTS
UNIT-I
Historical developments of Microbiology: Contributions of Leeuwenhoek, John Needham,
Lazzaro Spallanzani, Theodar Schwann, John Tyndall, Louis Pasteur, Robert Koch,
Winogradsky, (Winogradsky column), Beijerinck and Joseph Lister.
UNIT-II
Microbial Taxonomy: Whittaker’s five kingdom approach. Major characteristics used in
taxonomy – Morphological, Physiological and Metabolic, Genetic and Molecular- Numerical
taxonomy. Bergey’s manual of systematic bacteriology – first and second edition - and its
importance.
UNIT-III
Techniques in Microbiology: Methods of Sterilization - Physical and chemical agents,
radiation & filtration, Microbiological media: Enriched medium, enrichment medium,
transport medium, selective medium & pure culture techniques. Staining techniques - Simple,
Differential, Spores and Capsule.
UNIT-IV
Outline classification of Fungi and Algae: General structure and characteristics and
lifecycle: Fungi – Ascomycetes (Saccharomyces) Zygomycetes (Rhizopus) and
Deuteromycetes (Aspergillus) and Basidiomycetes (Agaricus). Algae: Chlorophyta
(Chlamydomonas) Rhodophyta (Polysiphonia).
UNIT-V
Exploitation of Microbes by Human: Microbes as sources of protein (Agaricus &
Spirullina)–Biofertiliser (Rhizobium), Biopesticide (Bt), Antibiotics (Streptomycin),
recombinant vaccines (FMD vaccine & interferons).
TEXT BOOKS:
1. Microbiology. Prescott, L.M.; J.P. Harley and D.A.Klein. 1993. 2nd edition. Wm.c.
Brown publishers.
254 Director, SBS
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Page No.14
2. Microbiology. Michael J. Pelczar JR.1993, Concepts and Applications McGraw-Hill.
Inc.
REFERENCE BOOKS
1. Microbiology. An introduction. Gerald J Tortora, Berdell R Funke, Christine L Case
(1995), 5th Edition; The Benjamin Cummings Publishing Co.Inc. USA.
2. Microbiology. Principles and Explorations. Jacquelyn G.Black (2008) 7th Edition;
John Wiley & Sons Pte. Ltd. Asia.
3. Biology of Microorganisms-Brock, TD. Smith D.W. and Madigan M.T. 1984.
4thedition.Englewood, cliffs. N.J. Prentice Hall.
4. Microbiology- Fundamentals and Applications. Ronald M.Atlas. 1989 II edition.
Maxwell Macmillan International Editions.
PEDAGOGY: Chalk and talk, OHP, PPT
255 Director, SBS
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Page No.15
SEMESTER – I
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper-II – Microbial Physiology and Metabolism
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about structure and functions of microbial cells,
biomolecule production and its nutritional requirements. The major aim is to make the
students to gain knowledge about physiology of microorganisms and their energy production.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the structure of prokaryotic and eukaryotic cells and transport of molecules. (K2)
CO2: Define the nutrition and growth of microorganisms along with types of culture
techniques. (K1)
CO3: Comprehend the different metabolic pathways, catabolism of biomolecules and
anaerobic respiration. (K2)
CO4: Detailing on important biosynthetic pathways in microorganisms. (K1)
CO5: Describe on pathways and energy generation in photoautotrophs and other
extremophiles. (K5)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 25
ESE – 75
256 Director, SBS
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Page No.16
CONTENTS
UNIT-I
Ultra structure of prokaryotic and Eukaryotic cells: Sub cellular structures – Cell wall,
Cell membrane, Cell envelope, Liposomes. Membrane transport: Diffusion, Active and
Passive transport, group translocation. Iron uptake and Sporogenesis.
UNIT-II
Nutrition and growth of microorganisms: Nutritional types of microorganisms, nutritional
requirements. Factors influencing the growth of microorganisms – Temperature, pH, Pressure
(Osmotic, Hydrostatic), nutrients, moisture and radiations. Physiology of growth–growth
curve and growth measurements. Types of culture techniques: Batch, Continuous,
Synchronous and Diauxic growth.
UNIT-III
Respiratory metabolism: EMP pathway, HMP Pathway, Entner-Doudoroff pathway,
Glyoxalate pathway, Krebs cycle- oxidative and substrate level phosphorylation, Electron
transport system and ATP production. Bioluminescence - Pasteur Effect; Fermentation of
carbohydrates-lactic acid,butyric acid and mixed acid. Lipid catabolism- oxidation and
αoxidation. Anaerobic respiration: Sulfur compounds, nitrate and CO2 as electron acceptors.
UNIT-IV
Biosynthesis of aspartate, pyruvate, histidine and serine amino acid families - purine and
pyrimidine nucleotides, salvage pathway- de novo pathway. Biosynthesis of fatty acids and
phospholipids. Biosynthesis of Gram positive and Gram negative cell wall.
UNIT-V
Photoautotrophs: Brief account of photosynthetic and accessory pigments-oxygenic-
anoxygenic photosynthesis autotrophic generation of ATP; fixation of CO2 - C3 pathway.
Chemolithotrophic metabolism: Iron, Hydrogen, Nitrogen and sulfur oxidations. General
account of Archaebacteria. Basic physiology of halophiles, thermophiles and methanogens.
TEXT BOOK:
1. Microbial Physiology - Albert G.Moat and John W.Foster. 1988. 4th ed. John Wiley
and sons.
257 Director, SBS
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Page No.17
REFERENCE BOOKS:
1. Microbial physiology and Metabolism - Caldwell, D.R. 1995. Brown publishers
2. Chemical Microbiology: An introduction to Microbial physiology- Rose.A.H. 1986
Wiley and sons.
3. Bacterial Metabolism - GottsChalk, G .1986, 2nd edition, NY.
4. Bacterial Metabolism - Doelle.H.W.2005.2nd edition. Elsevier publications.
PEDAGOGY: Chalk and talk, OHP, PPT
258 Director, SBS
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Page No.18
SEMESTER – I
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper III - Food and Dairy Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge on overview of food related microbes and its interaction with food as
substrate, food spoilage and food-borne illness manifested by food borne microbes, dairy
products and its fermentation.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basic Importance of studying Food and Dairy Microbiology. (K2)
CO2: Contamination and spoilage of foods and its control through preservation. (K5)
CO3: Exemplify the concepts of food borne diseases and detection methods. (K1)
CO4: Understanding the types of Food Fermentations and Introduction to Probiotics. (K2)
CO5: Explore the concepts of HACCP and its applications in food industry. (K3)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 PO3 PO4
CO1
CO2
CO3
CO4
CO5
CIA – 25
ESE – 75
259 Director, SBS
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Page No.19
CONTENTS
UNIT-I
Food and Dairy Microbiology: Food as a substrate for microorganisms-primary sources of
microorganisms in foods. Factors influencing microbial growth in foods- extrinsic and
intrinsic factors. Microorganisms important in Food Microbiology: Molds, Yeasts and
Bacteria- General Characteristics- Classification and importance.
UNIT-II
Contamination and spoilage: Cereals, vegetables and fruits, meat and meat products, Fish
and sea food - milk, Spoilage of canned foods. Principles of food preservation- Asepsis-
Removal of microorganisms, anaerobic conditions- High temperature- Low temperature -
Drying - Chemical preservatives (Benzoate, sugar and salt).
UNIT-III
Food borne diseases and microbial analysis in foods: Food borne infections and
intoxications (Bacterial and Fungal) - Botulism-Staphylococcal food poisoning-Salmnellosis,
Shigellosis, Aflatoxin, Patulin and Ochratoxin. Milk borne infection and intoxication -
Indicator organisms in food - Direct examination - microscopic culture techniques -direct
epifluorescent filter technique- enumeration methods - Viable & Total Count - Dye reduction
tests - Chemical methods- ATP bioluminescence and Radiometry- Immunological methods -
ELISA and RIA, Molecular methods – PCR.
UNIT-IV
Food fermentations: Microorganisms as food – Single cell protein – organisms as SCP –
production process– carbon substrates – SYMBA process, PRUTTEN process. Fermented
dairy products - Butter milk, Cream, Yoghurt, Kefir, Koumiss, Acidophilus milk and Cheese
production (Roquefort and Camembert). Food produced by microbes -Bread, Vinegar and
oriental fermented foods (Soy sauce, tempeh and miso). Introduction to Probiotics as foods-
therapeutic and nutritional value (Lactobacillus, Bifidobacterium).
UNIT-V
HACCP in Food Industry: Definition- principles of HACCP –Flow diagram development of
HACCP worksheet and plan form, HACCP audit in food processing plants, HACCP manual –
records for HACCP audit – formats for HACCP records –Applications and limitations of
HACCP.
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TEXT BOOKS
1. Food Microbiology. Adams MR and Moss MO, 2005, 1st Edition, Reprinted,
Published By New Age International (P) Limited, Publishers, New Delhi.
2. Food Microbiology. Frazier WC, Westhoff DC, 1988., TATA McGraw Hill.
REFERENCE BOOKS
1. Modern Food Microbiology. James M Jay, 2000. 4th Ed, CBS Publishers &
Distributors, New Delhi.
2. Basic Food Microbiology. Banwart GJ, 2004. 2nd Ed, CBS Publishers & Distributors,
New Delhi.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – I
COURSE: M. Sc., Applied Microbiology
PAPER TITLE: Paper IV - Molecular Genetics
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVES
To Demonstrate in-depth knowledge of what genes are and how they work; clarify the
mechanisms for the flow of information from genes to proteins, and how these processes are
regulated.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate the molecular machinery of living cells. (K3)
CO2: Describe the process of transcription in Prokaryotes and Eukaryotes. (K1)
CO3: Understand the regulation of gene expression in Prokaryotes and Eukaryotes. (K2)
CO4: Illustrate the mechanism of gene mapping. (K2)
CO5: Describe and identify the mechanism of mutations and its repair. (K1)
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CONTENTS
UNIT-I
Genes and Chromosomes: Mendelian principles, organization of genes, DNA Chemistry &
Structure, Physical and chemical properties of DNA, forms of DNA, DNA Replication
(Prokaryotes and Eukaryotes) - enzymes of replication, semi-conservative model of
replication, theta and rolling circle replication.
UNIT-II
Processing of genetic information: Non-coding RNA – Small, long and circular,
Transcription in prokaryotes and eukaryotes.RNA polymerases (RNAP I, II & III) - inhibitors
of RNAP. Post transcriptional modifications (for all types of RNA) - Capping,
polyadenylation and splicing mechanism, Transcriptional control of gene expression
(promoters, enhancers and CpG islands).
UNIT-III
Gene expression and regulation: Genetic code – Basic Features: Codons, Anticodons,
Universality, Overlapping, Non-overlapping and Redundancy. Translation (initiation,
elongation and termination & factors responsible), post-translational modifications,
Regulation of gene expression (lac and trp operon).
UNIT-IV
Mechanism of genetic exchange: Transformation- competence factors- mechanism of DNA
uptake, transduction- generalized (P1) and specialized (lambda), mapping by co-transduction
frequency, conjugation- F plasmid- F’- Hfr mapping- mapping of unselective recessive
markers, transposons (Tn10 and retrotransposons), genetic recombination.
UNIT-V
Mutation & Genetic disorders: Spontaneous- transition, transversion, missense, non-sense,
and frame shift mutations, Mutagens- physical and chemical- survival curve, mechanism of
repair- light and dark mechanism, SOS. Genetic disorders – β-thalassemia, Sickle cell anemia,
and Down syndrome. Cancer genetics: Loss of cell cycle control, oncogenes, tumor
suppressor genes and apoptosis. Abnormalities in DNA repair mechanisms.
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TEXT BOOKS:
1. Principles of Genetics. Gardner (2001), John Wiley & Sons Inc, New York
2. Principles of Genetics. Robert Tamarin, (1996) 5thEdition.WMC Brown publication,
Boston.
REFERENCE BOOKS:
1. DNA Structure and Function - Richard R. Sinden, (2012), Academic Press.
2. DNA Replication- Arthur Kornberg, 2nd Ed, (2005), University Science Books.
3. Genes IX - Benjamin Lewin, (2007), Jones & Bartlett Learning, New Jersey.
4. Molecular Cell Biology - Harvey Lodish, Arnold Berk, Paul Matsudaira, Chris A. Kaiser,
Monty Krieger, Matthew P. Scott, Lawrence Zipursky, and James Darnell, 8th edition (2016),
Published by W.H freeman and Company, New York.
5. Molecular Biology of the Gene. James D. Watson, Tania A. Baker, Stephen P. Bell&
Alexander Gann, 7th edition (2010), Published by Pearson Education PVT, Ltd. Singapore..
6. Microbial genetics by Stanley R Maloy, (1994), Jones and Bartlett Publishers.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – I
COURSE: M. Sc., Applied Microbiology
SUBJECT TITLE: Practical-I – Microbial physiology, Molecular genetics and Food
Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To divulge knowledge on the practical aspects on fundamentals, physiology of
microorganisms such as growth of microbes and their identification along with genetics of
microbes, quality features of food and dairy products.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Comprehend the basics of microbiology such as sterilization, media preparation,
isolation and differentiation of microorganisms. (K2)
CO2: Illustrate knowledge on isolation of anaerobes and to determine the growth of
microorganisms along with their identification by biochemical tests. (K2)
CO3: Understand the isolation of microorganisms responsible for food spoilage and to assess
the quality of milk. (K2)
CO4: Instantiate knowledge on the process of mutagenesis and operon system. (K2)
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Fundamentals of Microbiology
1. Sterilization – principles and applications.
2. Media preparation – Liquid and solid media, agar deep, slant and plate.
3. Pure culture techniques – streak plate, pour plate and spread plate.
4. Motility determination – SIM and hanging drop method.
5. Enumeration of microorganisms – bacteria, actinomycetes and fungi from soil.
6. Staining techniques – simple, differential, spore, negative and acid fast staining.
7. Lactophenol cotton blue mounting for identification of fungal morphology.
Microbial Physiology
8. Measurement of microbial growth and determination of generation time – Turbidimetric
method for bacteria.
9. Anaerobic culture technique – Wright’s tube method and McIntosh anaerobic jar
Method.
10. Biochemical characteristics of microorganisms
Indole test
Methyl red test
Voges-Proskauer test
Citrate utilisation test
Oxidase test
Catalase test
Urease test
Nitrate reduction test
Carbohydrate fermentation test (Glu, Suc, Lac and Mannitol) and TSI test
Food and Dairy Microbiology
11. Determination of milk quality – MBRT.
12. Isolation and enumeration of microorganisms from spoiled food – (Bacteria & Fungi).
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Molecular Genetics
13. Induced Mutagenesis – physical method.
14. Induction of lac operon (ONPG).
REFERENCES:
1. Experiments in Microbiology, Plant Pathology and Biotechnology – Aneja, K.R.
2005, 5th Edition. NAI Publishers.
2. Microbiology – A lab Manual. Cappuccino, Sherman, 2008, 6th Edition. Pearson
Education.
3. Microbiology – Practical Lab Manual. Kannan. K, 2002, Palani Paramount
Publishers.
4. Experimental procedures in Lifesciences. Rajan. S.
PEDAGOGY: Chalk and talk, OHP, PPT, Demonstration
267 Director, SBS
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SEMESTER – I
COURSE: M. Sc., Microbiology
SUBJECT TITLE: Paper VIII: Bioethics, Biosafety, TQM and IPR
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVES
To provide basic concepts and importance of bioethics and biosafety, TQM and IPR
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate Ethics and Ethical issues in GMO’s. (K3)
CO2: Describe the Biosafety levels of microbes, plants and animals. (K1)
CO3: Understand the Trade Quality Management. (K2)
CO4: Illustrate the IPR. (K2)
CO5: Describe the concepts of Plant breeding and its rights for protection. (K1)
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CONTENTS
UNIT-I
Bioethics in plant and animal genetic engineering & transgenics - Ethics of genetically
modified microbes, ethics in genetically modified food, ethical issues in human
biotechnology, ethics in stem cell research, gene therapy and biowarfare.
UNIT-II
Biosafety guidelines in India and international level, physical and biological containment,
biosafety levels for microbes, plants and animals, risk assessment and management of
laboratory research, risk assessment of biotechnological products, field trials with genetically
modified plants, planned introduction of genetically engineered microbes.
UNIT-III
Principles of TQM, Tools, steps, techniques and methods for TQM (Six sigma, charts,
Ishikawa diagram, tree diagram, RCA and PDCA cycle), Requirements for supplementing
TQM - steps for supplementing TQM - questionnaire - assessment through questionnaire,
mission statement, benefits of TQM, check list for implementing TQM, Case study.
UNIT-IV
Intellectual property rights- protection of biotechnological inventions, patents- types,
patenting of genes, biological organisms, plants, animals, microbes and transgenic organisms,
trade secrets, copyright, World Intellectual Property Rights organization (WIPO), GATT
(General agreement of tariff and trade), biodiversity bill of India.
UNIT-V
IPR for plant breeding-plant variety protection in India, plant breeder’s rights, farmer’s rights,
protection & implementation of plant varieties, farmer’s rights act, national gene fund,
Benefits of PBR, mechanism involved in terminator technology for seed protection,
technology for pure lines & hybrid seed protection, traitor technology.
TEXT BOOKS:
1. Intellectual Property Rights. Radhakrishnan R. and Balasubramanian, S. Excel
Books, I ed., 2008.
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2. Handbook of Indian Patent Law and Practice, Subbaram N. R.,S. Viswanathan
(Printers and Publishers) Pvt. Ltd., I ed., 1998.
REFERENCE BOOKS:
1. Bioethics and Biosafety in Biotechnology, Sree Krishna, V, New Age International
Publishers, I ed., 2007.
2. Technology Transfer, Goel Cohen, Sage Publications, 2004.
3. Twelve management skills for success- Ram Narain, Viva books private limited,
Chennai.
4. Across functional perspectives of TQM. Rao, Carr, Dambolena and Kopp. John wiley
and sons, Newyork, 1996.
5. Ethics in engineering, Martin. M.W. and Schinzinger.R. 3rd Ed, Tata McGraw-Hill,
New Delhi. 2003.
6. Biosafety issues related to transgenic crops, DBT guidelines, Biotech Consortium
India Limited, New Delhi, 2005.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – I
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective I- Group B: Quality Assessment in Pharmaceuticals
Total teaching hours/week: 5 Hrs
COURSE OBJECTIVE
This paper presents the study of various aspects involved in product quality and quality
assurance, the methodology necessary to establish and implement quality assurance program
for a manufacturing plant with good manufacturing practices.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand different types of antimicrobial drugs and its action. (K2)
CO2: Develop knowledge on microbial spoilage and sterile pharmaceutical products. (K6)
CO3: Comprehend the concepts of sterilization control and methods of sterility testing. (K2)
CO4: Identify criteria for qualified personnel, safety and identification methods along with
biofilms and disinfection agents. (K1)
CO5: Explore the perceptions on validation methods, regulatory requirements and auditing in
pharmaceutical industry. (K3)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
An introduction to pharmaceutical microbiology. Chemical growth control. Chemical
antimicrobial agents for external use, synthetic antimicrobial drugs, naturally occurring
antimicrobial drugs: Antibiotics. Antibiotics from prokaryotes, antiviral drugs, antifungal
drugs, antimicrobial drug resistance, the search for new antimicrobial drugs.
UNIT-II
Types of spoilage, Factors affecting microbial spoilage – assessment of microbial spoilage –
preservation. Ecology of microorganisms as it affects the pharmaceutical industry – Sterile
pharmaceutical products – injections, Non injectable sterile fluids, ophthalmic preparations,
dressings and implants.
UNIT-III
Sterilization control - methods of sterility testing- sterilization monitors and Quality assurance
of products. The microbiological quality and regulatory requirements for natural and
nutraceutical products, The regulatory control and quality assurance of immunological
products, Containment system integrity – sterile products, Regulatory guidelines
(microbiology) for veterinary antimicrobial products.
UNIT-IV
The role of the Qualified Person in microbiological quality assurance, Safety in microbiology,
Rapid enumeration and identification methods, Selection and use of cleaning and disinfection
agents in pharmaceutical manufacturing, Prevention and elimination of microbial biofilms in
the manufacturing environment using Clean-in-Place, Cleanroom design, operation and
regulatory standards.
UNIT-V
Microbiological quality assurance. Validation of aseptic processing and media fills,
International disinfectant testing protocols, Measurement of biocide effectiveness,
Microbiological quality and regulatory requirements for biotherapeutics and manufactured
products, The role of the microbiologist in HACCP, Auditing the pharmaceutical
microbiology department.
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TEXT BOOKS:
1. Pharmaceutical biotechnology - Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. andRussel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS:
1. Gene therapy - Templeton and Lasic (2000) Marcel and Dekkn.
2. The basic science of gene therapy - Richard C. Mulligan (1993) American
Association for the Advancement of Science.
3. Comprehensive biotechnology - Murray Moo-Young (Ed) Vol-3 (2004) Permagon
Press.
4. Pharmaceutical biotechnology - Purohit (2003) 2nded. Agrobios.
PEDAGOGY: Chalk and talk, OHP, PPT
273 Director, SBS
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SEMESTER – I
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective I- Group C: Basic Biological Sciences
TOTAL TEACHING HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about structure and function of cells, cellular
energetic, protein trafficking and cellular development. Understanding the structural and
functional aspects of the cell provide the student with a strong foundation in the molecular
mechanisms underlying cellular function.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand structure/function of prokaryotic and eukaryotic cells and their intracellular
organelles. (K2)
CO2: Develop knowledge on basic composition and association of various biomolecules. (K6)
CO3: Comprehend the physiology of microbes, animals and plants. (K2)
CO4: Gain insights on qualified personnel, safety and identification methods along with
biofilms and disinfection agents. (K2)
CO5: Appreciate views on origin and evolution of different life forms. (K4)
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CONTENTS
UNIT-I
Cell Biology: Structure and function of cells and intracellular organelles (of both prokaryotes
and eukaryotes), mechanism of cell division including (mitosis and meiosis) and cell
differentiation: Cell-cell interaction; Dosage compensation and mechanism of sex
determination.
UNIT-II
Biochemistry: Structure of atoms, molecules and chemical bonds; Principles of physical
chemistry: Thermodynamics, Kinetics, dissociation and association constants; Nucleic acid
structure, genetic code, replication, transcription and translation: Structure, function and
metabolism of carbohydrates, lipids and proteins; Enzymes and coenzyme; Respiration and
photosynthesis.
UNIT-III
Physiology: Response to stress: Active transport across membranes; Plant and animal
hormones: Nutrition (including vitamins); Reproduction in plants, microbes and animals.
UNIT-IV
Evolutionary Biology: Origin of life (including aspects of prebiotic environment and
molecular evolution); Concepts of evolution; Theories of organic evolution; Mechanisms of
speciation; Hardyweinberg genetic equilibrium, genetic polymorphism and selection; Origin
and evolution of economically important microbes, plants and animals.
UNIT-V
Environmental Biology: Concept and dynamics or ecosystem, components, food chain and
energy flow, productivity and biogeochemical cycles; Types of ecosystems, Population
ecology and biological control; Community structure and organisation; Environmental
pollution; Sustainable development; Economic importance of microbes, plants and animals.
TEXT BOOKS:
1. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology – P S Verma &
VK Agarwal., 2004 S. Chand & Sons. Ltd., New Delhi.
275 Director, SBS
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2. Fundamental Processes in Ecology. 2007. Wilkenson DM.
3. Terrestrial Ecosystems - Aber J.D. & Melillo J M. 1991
REFERENCE BOOKS:
1. Economic Zoology - Manju Yadav. Discovery publishing house, New Delhi.
2. Phycology - Lee R E., 1999.
PEDAGOGY: Chalk and talk, OHP, PPT.
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SEMESTER – II
COURSE: M.Sc., Applied Microbiology
SUBJECT TILTLE: Paper-V: Microbial Techniques
TOTAL TEACHING HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
By learning students can acquire the basic working principle and other instrumentation
aspects of various advanced analytical instruments used in various fields of microbiology.
Analyzing the microbial biomolecules for pathogen identification to improve the quality and
precision of microbial diagnostic techniques.
COURSE OUTCOME AND ITS COMPETENCY LEVEL
CO1: Review the concepts of basic type of microscopes and also to evaluate the efficiency of
new and advanced microscopic types in analyzing the nano-size based aspects of microbes
and the application of microbial products in various scientific fields. (K2)
CO2: Understanding the advanced and basic types of spectroscopic types for microbial
product analysis and exploring their working principle. (K2)
CO3: Analyzing the microbial biomolecules using molecular techniques can help the students
to understand the microbial living system better. (K4)
CO4: Evaluate the pathogenic nature of microbes using their nucleic acid content which can
help the man kind to be secure from pathogens present in their basic needs like water and
food. (K5)
CO5: Molecular differential diagnosis, the growing modernized diagnostic technique can be
understood by students and the basic needs for installing molecular differential diagnostic
laboratory is also described to the students. (K2)
CIA – 25
ESE – 75
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CONTENTS
UNIT-I
Microscopy: Basic principle and applications of high resolution-TEM (HRTEM), atomic
force microscopy, scanning tunneling microscopy, energy dispersive x-ray analysis.
UNIT-II
Spectroscopy: Basic principle and applications of Raman spectroscopy, IR spectroscopy, X-
ray photoelectron spectroscopy, FTIR, wide-angle X-ray diffraction spectroscopy, photon
correlation spectroscopy.
UNIT-III
Molecular detection and identification of microbes-I: Restriction endonuclease analysis -
pulse field gel electrophoresis (PFGE), denaturing gradient gel electrophoresis (DGGE),
amplified fragment length polymorphism (AFLP), terminal restriction fragment length
polymorphism (T-RFLP), capillary electrophoresis single-strand confirmation polymorphism
(CESSCP), Metagenomics.
UNIT-IV
Molecular detection and identification of microbes-II: Biosensors-detection of bacterial
UTI using electrochemical DNA biosensors, HIV detection using surface plasmon resonance
(SPR)-based optical techniques, light-addressable potentiometric sensor (LAPS) for food-
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borne pathogen detection. Flow cytometry in microbiology, microarrays, catalysed reporter
deposition-fluorescence in situ hybridization (CARD-FISH).
UNIT-V
Molecular diagnostic techniques: Rapid antigen testing – Lateral flow immuno-
chromatography, molecular diagnosis of STD - probe hybridization, gene amplification, ligase
chain reaction (LCR), strand displacement amplification. Molecular diagnosis of drug
resistant microbes – Mycobacterium tuberculosis and MRSA, VISA, VRSA, Molecular
differential diagnosis (MDD) of infectious diseases.
TEXT BOOKS:
1. Microbiology. Prescott, L.M.; J.P. Harley and D.A.Klein. 1993. 2nd edition. Wm.c.
Brown publishers.
2. Microbiology. Michael J. Pelczar JR.1993, Concepts and Applications McGraw-Hill.
Inc.
REFERENCE BOOKS:
1. Advanced techniques in Diagnostic Microbiology-Yi-Wei Tang, Charles W.
Straston, 2006, Springer.
2. Biomedical Nanostructures-Kenneth, E.,Gonsalves, Cato, L., Laurencin, Craig, R.,
Halberstadt, Lakshmi S. Nair, 2008 JohnWiley&Sons, Inc.
3. Practical Biochemistry Principles and Techniques-Wilson K, Walker J. 1995.
Cambridge University Press.
PEDAGOGY: This subject can be taught using chalk and talk method, over-head projector
usage, power point slides presentation using LCD projector.
279 Director, SBS
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Page No.39
SEMESTER – II
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper VI - Industrial Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a broad knowledge in the field of industrial Microbiology and its
processes involved in variety of industries. To elaborate the students on the fundamentals of
upstream and downstream processing and production of microbial products of commercial
importance.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the screening and strain improvement of industrially important
microorganisms. (K2)
CO2: Recognize knowledge on fermentors, basic parts, types and its functioning. (K2)
CO3: Appraise insights on steps involved in upstream process and different types of
fermentation. (K5)
CO4: Summarize knowledge on the downstream processes involved in recovery and
purification of fermentation products. (K2)
CO5: Comprehend the steps followed in production of commercially important microbial
products. (K2)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
General concepts of Industrial microbiology: Isolation of industrially important
microorganisms – Screening of microorganisms for industrial products (Primary and
Secondary) Characteristics of industrially important microbes. Strain development strategies –
natural variants, mutation and recombination. Immobilization of cells. Preservation of
microorganisms for industrial products.
UNIT-II
Fermentors and its functions: Basic components – asepsis and containment requirements –
temperature control – aeration and agitation systems – mass transfer of oxygen. Concepts of
newtonian and non–newtonian fluids – sterilization of fermenter, air supply and medium;
aseptic inoculation methods – sampling methods. Types of fermentor: – Cylindroconical,
airlift, deepjet, fluidised bed tower and cyclone.
UNIT-III
Fermentation systems: Upstream processing – Media formulation – Antifioam agents –
Development of inocula for bacterial and mycelial fermentation. Types of fermentation –
Submerged and solid state fermentation. Fermentation systems – Batch, Fed batch and
continuous. Scale up of fermentation processes.
UNIT-IV
Downstream processing: Recovery and purification of fermentation products – precipitation
methods – filtration devices and filter aids – centrifugation – cell disruption methods – liquid–
liquid extraction – solvent recovery – chromatography – two–phase aqueous extraction –
super– critical fluid extraction – ultrafiltration – crystallisation, whole broth processing and
lyophilisation.
UNIT-V
Production of microbial products: Organic acids – citric acid, lactic acid and acetic acid.
Amino acids – glutamic acid and lysine. Alcohols and beverages – ethanol, butanol, beer and
wine. Enzymes – proteases, amylases, lipases. Antibiotics – penicillin and streptomycin. (any
one anti-fungal antibiotic)
281 Director, SBS
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TEXT BOOKS:
1. Industrial Microbiology. Patel AH. (2005). Macmillan India Ltd. New Delhi.
2. Industrial Microbiology. LE Casida, Jr. (2005). New Age International (p) Ltd. New
Delhi.
REFERENCE BOOKS:
1. Industrial Microbiology. Samuel C Presscott and Ceril G Dunn. (2005). Agrobios
(India), Agro house, Jodhpur.
2. Microbial Technology. H. J. Peppler and D. Perlman. (2004). 2nd edition, Academic
press.
3. Principles of Fermentation technology. Stanbury PF, A Whitaker and SJ Hall 1997.
2nd edition, Pergamon press.
PEDAGOGY: Chalk and talk, OHP, PPT
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Page No.42
SEMESTER – II
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper VII - Recombinant DNA Technology
TOTAL TEACHING HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To provide the students about the basic steps in cloning, the enzymes used and the screening
techniques followed in the Recombinant DNA Technology.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand the enzymes and the prokaryotic vectors which are useful in cloning (K2).
CO2: Understand the use and properties of eukaryotic vectors. Also able to understand the
different methods used for screening of the recombinant clones (K2).
CO3: Understand the sequencing techniques, construction of libraries, and DNA
amplification techniques (K2).
CO4: Understand the use of site directed mutagenesis and the use of DNA in diagnosis of
genetic disorder and infectious disease (K2).
CO5: Understand the methods for creating transgenic plants, animals and microbes (K2).
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CONTENTS
UNIT-I
Gene cloning: Basic steps in cloning, Gene analysis techniques: Isolation of DNA and RNA.
Enzymes used in gene manipulation- Restriction enzymes, Reverse transcriptase, End
modifying enzymes, Methylases, Ligation- DNA ligases, use of linkers & adaptors.
Vectors: Prokaryotic vectors: Plasmid as cloning vectors – Features, pBR322, pUC,
Bacteriophage vectors-λ (insertional and replacement vectors), M13, Cosmids, Phagemids,
Phasmids and BAC.
UNIT-II
Eukaryotic vectors: Yeast Vectors-Yeast integrative vectors, YAC. Animal vectors-SV40,
Retroviral vectors, Plant vectors- Ti Plasmid, plant viral vectors. Specialized vectors-
Expression vectors, Shuttle vectors. Gene Transfer Methods, Selection and Analysis of
recombinant Clones- Direct screening – insertional inactivation and visual screening. Indirect
screening – Nucleic acid blotting (Northern and Southern), Colony, Plaque and Dot blot
hybridization, immunoscreening.
UNIT-III
Construction of Genomic and cDNA Libraries- Strategies for construction of genomic
libraries, Mapping techniques- Chromosome walking and jumping, Restriction mapping-
RFLP, RAPD. DNA sequencing– Maxam and Gilbert method, Sanger’s method. PCR-
Principle, types and Applications.
UNIT-IV
Site directed mutagenesis- Methods, Screening of recombinants for SDM by SSCP and
heteroduplex analysis, DNA foot printing and finger printing, Nucleic acid micro arrays-
Affimetrix®. DNA based diagnosis of genetic disorders- Cystic fibrosis, Diagnosis of
Infectious diseases using DNA- Tuberculosis, Gene therapy- types, strategies and
applications.
UNIT-V
Engineering Genes: Engineering microbes - Insulin production in E. coli, Production of
Vaccines for Hepatitis B Virus using yeast. Engineering plants - Delayed ripening, Pathogen
(insect) resistance. Engineering animals - Tissue plasminogen activator production, embryonic
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stem cells and nuclear transfer methods. Applications of Transgenesis- Transgenic microbes
as biopesticides, transgenic cattle - increased milk production, transgenic fish - improving
growth rate, transgenic sheep - enhanced wool production.
TEXT BOOKS:
1. Gene cloning - An introduction. T. A. Brown, 3rd edition, (1995), Stanley Thomas
publications limited.
2. Principles of Gene Manipulation and Genomics. S.M. Primrose and R.M Twyman,
7th edition, (2006), Blackwell Publishing, Australia.
REFERENCE BOOKS:
1. From Genes to Clones. Ernst. L. Winnacker, 22nd edition, (2003), Panima publishing
Corporation, New Delhi
2. Genes VIII. Benjamin Lewin (2003), Pearson Education Corporation, New Jersy.
3. Essentials of Molecular Biology. Freifelder, George M. Malacinski, 4th Edition,
(2008), Narosa Publishing House, New Delhi.
4. Recombinant DNA technology. J.D. Watson, 2nd edition, (2001), WH Freeman and
Company, New York.
5. Molecular Biotechnology. Glick and Pasternak (1996), Panima Publishing
Corporation, New Delhi.
6. Molecular Cloning: A Laboratory Manual. Sambrook J, Fritsch EF, and Maniatis T
(2000), Coldspring Harbor Laboratory Press, New York.
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SEMESTER – II
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective I: Group A: Research Methodology and Bioanalytical
Techniques
Number of Hours/Week: 5 Hrs
COURSE OBJECTIVE
To impart knowledge about basics of research methodology, principles and applications of
different instruments, use of statistics in research, understand concepts to handle basic
instruments.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand the basics of research methodology. (K2)
CO2: Develop knowledge on collection, classification and tabulation of data. (K6)
CO3: Exemplify the concepts of Sampling distribution. (K1)
CO4: Determining views on Microscopy, Spectroscopy and Chromatography. (K4)
CO5: Explore the concepts of different instrumental techniques. (K3)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
Introduction to research methodology: Defining the research problem, significance of
research, Stages in research - formulation of hypothesis, objectives of research,
experimental/research design, literature collection, literature citation, investigation, data
collection and analysis, research report, format of thesis, manuscript/research article.
Interpretation and report writing and bibliography.
UNIT-II
Organizing a statistical survey - Planning and execution; Source of data - Primary and
secondary data; Data Collection - methods; Classification and tabulation of data;
Diagrammatic & graphical representation of data. Sampling methods; size of sample.
Measures of central tendency: arithmetic mean, median, mode, quartiles, deciles and
percentiles. Measures of variation: standard deviation. Correlation analysis: Scatter diagram,
Karl Pearson's coefficient of correlation and Spearman's rank method. Regression analysis.
UNIT-III
Sampling distribution and test of significance - Null and alternate hypothesis; Testing of
hypothesis; errors in hypothesis testing; standard error and sampling distribution; sampling of
variables (large samples and small samples). Students’‘t’ test. Chi - square test and goodness
of fit. Analysis of variance: one way and two-way classification, Duncan’s Multiple Range
Test.
UNIT-IV
Methods in Microscopy: Principle and applications of Light, fluorescence, phase contrast,
Confocal, electron microscopy and scanning tunnelling microscopy. Spectrometry and
Spectroscopy: Colorimetry, UV-Visible, Principle of IR and atomic absorption spectroscopy.
Chromatography - Principle and applications: Paper, Thin layer, Ion exchange, Gel filtration,
Affinity, GC, HPLC, GC-MS and LC-MS.
UNIT-V
Centrifugation: Principle and types of centrifugation. Electrophoretic techniques: Agarose,
PAGE, isoelectric focusing and 2D gel electrophoresis, Southern, Northern and Western
blotting techniques. Radiolabeling techniques: An overview of radioisotopes used in biology,
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their detection and measurement; molecular imaging of radioactive material and safety
guidelines.
TEXT BOOKS:
1. Research Methodology, Methods and techniques. C. R. Kothari, New Age
publication
2. Statistical Methods. S.P. Gupta, Sulthan Chand & Sons
3. Principles & Techniques of Practical Biochemistry. Keith Wilson, Kenneth H.
Goulding, Cambridge University Press.
REFERENCE BOOKS:
1. Instrumental methods of chemical analysis. B. K. Sharma, 11th edition, Blackwell
publications.
2. Statistical Methods. Stephen W. Looney (Humana publications).
3. Biostatistics: A Methodology for the Health Sciences. Gerald Van belle, 2nd Edition,
Wiley – Interscience publication.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M. Sc., Applied Microbiology
SUBJECT TITLE: PRACTICALS-II – Microbial Techniques, Industrial Microbiology
and Recombinant DNA Technology
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This subject provides an overview of instruments, its working principle and operation of the
instruments along with the application in the field of microbiology and its laboratory
techniques. It also covers the fundamental aspects and principles of microbial analysis
including their usefulness in industrial and molecular biology. Students would be aware of
handling the microbes, analyzing and producing product using the microbial isolates.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Demonstrate the analytical techniques for amino acids, carbohydrates and protein. (K3)
CO2: Describe the process of production of alcoholic beverages and enzymes. (K5)
CO3: Understand the production process for antibiotics and organic acids. (K2)
CO4: Illustrate various nucleic acid methods. (K2)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
Microbial Techniques:
1. Chromatographic techniques: Paper, TLC.
2. Estimation of sugar by DNSA method.
3. Estimation of protein –Lowry et al., method.
Industrial Microbiology:
1. Wine production and analysis of sugar and alcohol content
2. Production of extracellular enzyme by submerged fermentation
a. Protease
b. Amylase
3. Water quality test-MPN and Membrane Filtration.
4. Production and quantification of Antibiotics-Penicillin.
5. Production of citric acid
6. Analysis of Penicillin activity against bacterial strains.
Recombinant DNA Technology:
7. Isolation of nucleic acid from bacteria.
8. Isolation of bacterial plasmids.
9. Restriction digestion of bacterial DNA
10. Ligation of restricted bacterial DNA
11. Southern blotting for DNA (DEMO)
12. Transformation
TEXT BOOKS:
1. Molecular Cloning: A Laboratory Manual - Sambrook, J, Fritsch, EF and Maniatis
(2000), Coldspring Harbour Laboratory Press, New York.
2. Experiments in Microbiology, Plant pathology & Biotechnology - Aneja,K.R.
(2005), 5thed. NAI Publications.
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3. Microbiology, A lab manual - Cappuccino, Sherman (2008), 6th ed. Pearson
Education.
REFERENCE BOOKS:
1. Practical Biochemistry Principles and Techniques- Wilson K, Walker J. (1995),
Cambridge University Press.
2. Advanced techniques in Diagnostic Microbiology- Yi-Wei Tang, Charles W.
Straston, (2006), Springer.
3. Genetic analysis of pathogenic bacteria – Stanley R. Maloy, Vally J. Stewart,
Ronald K. Taylor (1996), Coldspring Harbour Lab Press
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Applied Microbiology,
SUBJECT TITLE: Elective II: Bioinformatics
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
To impart knowledge about various tools in bioinformatics, understand basic concepts to
determine the alignment between the sequences and to enable the students to learn the basic
computational skills in bioinformatics-sequence analysis.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Understand the basic bioinformatics concepts. (K2)
CO2: Develop knowledge on biological databases. (K6)
CO3: Exemplify the concepts of file format, data storage and retrieval in bioinformatics. (K2)
CO4: Determining views on sequence alignment and how structure can be visualized. (K4)
CO5: Explore the concepts of drug discovery, SNPs and molecular docking studies in
bioinformatics. (K6)
MAPPING WITH PROGRAM OUTCOMES
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CIA – 25
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CONTENTS
UNIT-I
Introduction to bioinformatics and data generation: Bioinformatics and its relation with
molecular biology. Scope of Bioinformatics, Bioinformatics and Internet, Useful
Bioinformatics sites on WWW, Introduction to single letter code of aminoacids, Symbols used
in nucleotides, Data retrieval- Entrez and SRS. Tools in Bioinformatics (FASTA, BLAST,
BLAT, RASMOL), databases (GENBANK, Pubmed, PDB). Applications of Bioinformatics.
UNIT-II
Biological Database: Types, introduction of Biological Databases: Nucleic acid databases
(NCBI, DDBJ and EMBL). Protein databases (Primary, Composite and Secondary).
Carbohydrate databases. Specialized Genome databases: (SGD, TIGR and ACeDB). Structure
databases (CATH, SCOP and PDB). Ligand Databases: (Chembank and Ligand Explorer).
Sequence retrieval (Demo).
UNIT-III
Data storage, retrieval, and interoperability: Flat files, relational, object oriented databases
and controlled vocabularies. File Format- (Genbank, DDBJ, FASTA, PDB, SwissProt).
Importance of PERL and PHYTHON (BIOPHYTHON).
UNIT-IV
Sequence Alignments and Visualization: Introduction to Sequences, Alignments and
Dynamic Programming: Local alignment and Global alignment (algorithm and example),
Pairwise alignment - BLAST (Demo) and FASTA Algorithm; Multiple sequence alignment -
Clustal W algorithm (Demo) and Phylogenetics (Building Phylogenetic trees) (Demo). 3D
structure viewers – Rasmol (Demo), SPDBv, Chime, Cn3D and PyMol.
UNIT-V
Introduction to Drug discovery: Introduction: Areas influencing drug discovery, ADME
properties, Molecular Docking, Pharmacogenetics and Pharmacogenomics applications,
Analysis of Single Nucleotide Polymorphisms, an important parameters in drug discovery.
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TEXT BOOKS:
1. Bioinformatics methods and applications: Rastogi.S.C et al., 1993. Prentice Hall of
India Pvt Ltd, NewDelhi, 4th ed., India.
REFERENCE BOOKS:
1. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. Andreas
D. Baxevanis (Editor) and B.F. Francis Ouellete (Editor). Third Edition.
2. Bioinformatics Sequence and Genome Analysis, 2004. David W. Mount, 2nd
Edition.
3. Trends in bioinformatics, 2006. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
4. Principles of Bioinformatics, 2005. Dr. P. Shanmugavel, Pointer publishes, Jaipur.
5. Introduction to Bioinformatics, Attwood A.T and D.J Parry-smith, Pearson
education ltd.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective II- Group B: Communicable and Non-communicable
Diseases
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper deals with the different types of diseases that are communicable and non
communicable. In addition, this field of science studies various clinical applications of
microbes for the improvement of health and on the aspects of the interrelationship between
infectious agents and their hosts. On successful completion of the subject, the student will
know about the mechanism of infection and its types.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Detailed account on respiratory tract infections. (K1)
CO2: Understandings on intestinal infections. (K2)
CO3: Comprehend the types of vector borne infections. (K2)
CO4: Defining the views on mycological infections. (K1)
CO5: Understand the concepts of non-communicable diseases. (K2)
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CONTENTS
UNIT-I
Respiratory infections – Influenza, Mumps, Measles, Rubella, Acute respiratory infections
and TB.
UNIT-II
Intestinal infections – Polio, Cholera, Acute diarrhea diseases, Food poisoning, Typhoid,
Amoebiasis Ascariasis, Hook worm, Tapeworm, Pinworm infections.
UNIT-III
Vector borne Infections – Leprosy, STD – AIDS – Diagnostic Techniques and Treatment.
UNIT-IV
Superficial mycoses – Dermatophytoses – Opportunistic fungal infections – Candidiasis –
Diagnostics Techniques and Treatment.
UNIT-V
Non-communicable diseases – Hyper Tension – Diabetes – Coronary Heart diseases – Cancer,
Obesity, Blindness, Accidents – Preventive measures.
TEXT BOOKS:
1. Textbook of Microbiology – Ananthanarayanan, R. and JayaramPanicker, C.K.
(2005) 7th edition.Orient Longman Pvt., Ltd.
2. Textbook of Medical parasitology – Subash Chandra Parija, 2nd edition. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, MelnickandAdelberg’s (2001). 22nd edition.
McGraw Hill Medical Publication Division.
2. Medical Microbiology – David Greenwood. Richard Slack and John Peutherer (2000)
15th ed. ChurchHill Living stone Publication.
3. Medical Parasitology – Chatterjee K.D. (2007). 7th edition.
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4. Medical Microbiology – Patric R. Murray (1990) Mosby Publications.
5. Textbook of Medical parasitology – Jayaram Panicker C.K. (2004) JP Brothers,
Medical publishers, New Delhi.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
COURSE: M.Sc. Applied Microbiology
SUBJECT TITLE: Elective II- Group C: Biophysics and Biochemistry
NUMBER OF HOURS/WEEK: 5 Hrs
COURSE OBJECTIVE
This paper provides a thorough knowledge about the basic biophysical and biochemical
techniques involved in the field of biological sciences. Students after completion of this paper
will be learning on the methods used for the analysis of biomolecules.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL
CO1: Detailed account on principles of biophysical methods. (K2)
CO2: Insights on principles of nucleic acid techniques. (K2)
CO3: Comprehend the techniques involving radioactive isotopes. (K2)
CO4: Defining the views on metabolism and catabolism of sugars, amino acids, lipids, fatty
acids and sterols. (K1)
CO5: Understand the molecular aspects of cancer. (K2)
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT-I
Principles of biophysical methods used for analysis of biopolymer structure, X-ray diffraction,
Hydrodynamic methods; plasma emission spectroscopy.
UNIT-II
Principles and techniques of nucleic acid hybridization and Cot curves; Sequencing of
Proteins and nucleic acids; Methods for measuring nucleic acid and protein interactions.
Structural polymorphism of DNA, RNA and three dimensional structure of tRNA.
UNIT-III
Principles and applications of tracer techniques in biology; Radiation dosimetry; Radioactive
isotopes and half-life of isotopes;Effect of radiation on biological system.
UNIT-IV
Interconversion of hexoses and pentoses; Amino acid metabolism; Coordinated control of
metabolism; Oxidation of lipids; Biosynthesis of fatty acids; Triglycerides; Phospholipids;
Sterols, Group transfer and Coupled reactions.
UNIT-V
Biochemistry and molecular biology of cancer; Oncogenes; Chemical carcinogenesis; Genetic
and metabolic disorders; Hormonal imbalances; Drug metabolism and detoxification.
TEXT BOOKS:
1. Introduction to Biophysics - Cortell
2. Molecular and Cellular Biophysics - Meyer B Jackson (2006), Cambridge)
3. Physical Chemistry for Life Sciences - Peter Atkins and Julio de Paula, 2006, Oxford
Press.
4. Biochemistry - Strayer
5. Molecular Cell Biology - Lodish
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REFERENCE BOOKS:
1. Molecular and Cellular Biophysics - Meyer B Jackson (2006), Cambridge)
2. Text Book of Biophysics - R N Roy, New Central Agency (P) Ltd, Culcutta
3. Physical Chemistry for the Biosciences - Raymond Chang,(2004), University book
4. Science Biological Thermo dynamics - Donald, T Hayine, (2007), Cambridge
5. The Cell Molecular Approach - G Cooper and R Hausman (2007) ASM Press
6. Molecular Biology - D Roberties, 8th edition. SAE.
PEDAGOGY: Chalk and talk, OHP, PPT
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SEMESTER – II
MODULAR COURSE-MODULE 1
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper I: Introduction to Clinical Laboratory Technology
NUMBER OF HOURS/SEMESTER: 30 Hrs
COURSE OBJECTIVE
To gain knowledge on the clinical assessment of various diseased conditions and routine test
followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: To understand the basics of clinical laboratory. (K2)
CO2: Learn about the preparation of reagents and its storage. (K2)
CO3: Understand the importance of biological samples. (K2)
CO4: To know about the reporting of tests. (K4)
CO5: Disposal of biomedical wastes. (K6)
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CONTENTS
UNIT-I
Basic laboratory principles - Code of conduct of medical laboratory personnel.The use of the
laboratory - Basic laboratory principles - Organization of Laboratory Functional components
of clinical laboratories (Cleanliness – Methods of hand wash, Cleaning of lab instruments,
Laboratory floors and table tops, precautions to be taken WRT patients, reports, analysis).
Basic needs of clinical laboratory technician, awareness of soft skills. Safety measures -
Medical laboratory professional and professionalism in laboratory workers - clinic borne
infection and personnel hygiene.
UNIT-II
Reagent preparation: preparation of molar, normal, percent solutions. Buffers, Acid, Base
solutions and its storage. Common Laboratory Equipment’s use, principle and maintenance-
spectrophotometer, Incubator, Hot Air Oven, Water Bath Centrifuge, Autoclave ,Microscopy.
Glassware – Description of Glassware, its use, handling and care.
UNIT-III
Basic Steps for Specimen collection-types of specimens- Requirement of Collection -
methods of Collection of biological fluids - Anticoagulants - Preservation of samples -
Chemical preservatives . An overview on the transportation of the samples.
UNIT-IV
Process of analysing the specimens- labeling the samples. Maintaining the sample record.
Colour code for the samples. Reporting results: a. Basic format of a test report, b. Release of
examination results c. Alteration in reports Quality Management system : Internal and
External quality control. Accuracy, Precision, and Reference value. Levi-Jenning’s chart (LJ).
UNIT-V
Biomedical waste management in a clinical laboratory: Disposal of used samples, reagents
and other biomedical waste - colour coding as per guidelines.
TEXT BOOKS:
1. Medical laboratory technology Kanai L. Mukherjee, 2007, Vol.1.Tata McGraw
Hill
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2. Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical laboratory technology Vol.1 Kanai L. Mukherjee, 2007,. Tata McGraw Hill.
PEDAGOGY: Chalk and Talk, OHP and PPT.
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SEMESTER – III
MODULAR COURSE-MODULE 1
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper II: Techniques in Clinical Laboratory Technology
NUMBER OF HOURS/SEMSTER: 30 Hrs
COURSE OBJECTIVE
To gain the knowledge on the clinical assessment of various diseased conditions and routine
test followed in the laboratory with the outline of clinical laboratory.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: Understand the procedures of clinical laboratory. (K2)
CO2: Learn about the preparation of reagents and its storage. (K2)
CO3: Understand the importance of biological samples. (K2)
CO4: Recognize histopathogical procedures. (K1)
CO5: Distinguish about blood banking procedures. (K4)
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CONTENTS
UNIT-I
Basic Hematological diagnosis and Clinical Chemistry: Preparation of Blood Smears-
method (Coverslip, spreader slide and Wedge), Types and causes of poor blood smears (in
diseased condition), Staining Techniques- Leishman’s stain, Wright’s stain, Field’s stain and
Romanowsky stains, Criteria for a good stain: Problem encountered during staining –
Troubleshooting. Total cell count: RBC, WBC and Platelet. Estimation of hemoglobin, PCV
& MCV, MCH, MCHC. Erythrocyte sedimentation Rate (ESR)-Westergen method and its
importance, Differential count, Determination of bleeding time, clotting time and prothrombin
time. Clinical Chemistry: Blood sugar Analysis- glucometer based analysis, Fasting,
Random, Post Prandial, HbA1C, and GTT.
UNIT-II
Collection of Urine - Biohazard management - Routine urine analysis - Colour - Clarity -
Odour - Volume - Chemical Examination - Benedict’s Test - Fehling’s test - Chemistrip
method - Test for Protein in Urine - Heat and Acetic Acid Test - Sulphosalicylic Acid Test -
Heller’s Test. Rotheral’s test, Gerhardt’s test, Fouchet’s Test – Hay’s sulphur test- Test for
Hematuria - Benzidine Test - Guaiacum Test - Gregersen’s Test. Microscopic Examination of
Urine: Crystals, casts and cells. Examination of stool- physical, chemical & microscopic
examination
UNIT-III
Body Fluids: Characteristics of Cerebrospinal Fluid, Synovial fluid, Pleural fluid, Pericardial
fluids, Peritoneal fluids and amniotic fluid. Semen analysis- physical, chemical & microscopic
examination, sperm count and motility.
UNIT-IV
Introduction to Histopathology- Basic steps for Tissue Processing- Fixing, Embedding-
methods- Microtomy- Staining- Mounting, methods of decalcifications –cryopreservation -
Equipments - Microtome -Types, Uses, Parts, different types of microtome knives, care &
maintenance. Automated tissue processor – components-Staining Methods - Hematoxylin &
Eosin stain -Mounting –visualization. Cryostat.
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UNIT-V
Blood banking: Blood Group (ABO & Rh) – methods of grouping & reverse grouping. Basic
blood banking procedures – collection of blood, anticoagulants used, cross matching, different
screening tests including Coomb’s Test (direct and indirect), preparation of different blood
components for use and how to serve a requisition. Preparation of red cell suspension. Blood
transfusion hazards - hemolytic disease of newborn. Apheresis.
TEXT BOOKS:
1.Tietz Text book of Clinical Chemistry and Molecular Diagnosis, Burtis A. Carl
and Edward R. Ashwood, David E. Burns fourth edition, W.B. Saunders Company.
2.Medical Laboratory Technology- Ramnik Sood –5th edition, 2006.
3.Handbook of Histopathological and Histochemical technique -C.F.A.Culling.3rd
edition- Butterworths. London.
4.Textbook of MLT, P.B.Godkar. 2nd edition. 2003, Bhalani publication.
REFERENCE BOOKS:
1. Practical Clinical Biochemistry - Harold Varley (2005). 4th Edition, CBS Publishers
and Distributors.
2. Medical laboratory technology. Vol.1 Kanai L. Mukherjee, 2007,.Tata McGraw Hill
PEDAGOGY: Chalk and Talk, OHP and PPT.
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SEMESTER II
MODULAR COURSE - MODULE II
COURSE: M.Sc., BIOCHEMISTRY
SUBJECT TITLE: PAPER I: HUMAN PHYSIOLOGY
NUMBER OF HOURS/WEEK: 30 HRS
COURSE OBJECTIVE: To provide the students with in-depth knowledge on the functions
of major organs of human body.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO1: To Understand the structure and functions of cells (K2).
CO2: To understand the mystery of complicated function aspects of individual organs in the
body (K2).
CO3: To understand the principles of human physiology (K2).
CO4: To distinguish the cells involved in each system (K4).
CO5: To exemplify the knowledge on the regulation of body functions (K2).
MAPPING WITH PROGRAM OUTCOMES
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CONTENTS
UNIT I
Human Anatomy and Functions- Cell and their functions – Types of muscles and mechanism
of muscle contraction – Immune system – Blood components.
UNIT II
Anatomy and Physiology of Cardiovascular system - Anatomy and Physiology of central
nervous system - Anatomy and Physiology of respiratory system.
UNIT III
Anatomy and physiology of Digestive system, Anatomy and Physiology of excretory system.
UNIT IV
Anatomy and Physiology of sensory organs, Anatomy and Physiology of endocrine system.
UNIT V
Anatomy and Physiology of reproductive system – Menstruation – pregnancy.
REFERENCES:
1. Human Physiology - Chaterjee C C , Vol I & II, 11th edition.
2. Review of Medical Physiology - Ganong, , 24th Edition.
3. The Anatomical Basis of Clinical Practice- Gray's Anatomy, 40th Edition.
4. Textbook of Human Anatomy - Ranganathan T S, 5th edition.
5. Text book of Medical Physiology – Sembulingam and PremaSembulingam– 6th
Edition, Jaypee Brothers Medical Publishers (P) Ltd.
308 Director, SBS
Anx.PAMB11, 1M.Sc.AMB (CMS College) 2017 2017-19 batch
Page No.68
SEMESTER – II
COURSE: M. Sc., BIOCHEMISTRY SUBJECT TITLE: MODULE II – PAPER II CLINICAL BIOCHEMISTRY
NUMBER OF HOURS: 30 Hrs
COURSE OBJECTIVE: Understanding the diagnostic importance of diseases and disorders.
COURSE OUTCOMES AND ITS COMPETENCY LEVEL:
CO 1: To understand the disorders and test associated with various organs (K2).
CO 2: To understand the gastric and pancreatic function tests (K2)
CO 3: To understand the importance of minerals and acid base balance in human system.
(K2)
CO 4: To assess the abnormalities with respect to various hormones (K5)
CO 5: To enable, distinguish and interpret the diseases. (K4)
MAPPING WITH PROGRAM OUTCOMES
PO1 PO2 P03 PO4
CO1
CO2
CO3
CO4
CO5
CONTENTS
UNIT I:
An overview of metabolism of carbohydrates, proteins, lipids and nucleic acids – Lipid profile
–Biochemical test for Blood glucose – Fasting, Post prandial, GTT and Hemoglobin A1C,
Renal function test (RFT).
CIA – 10
ESE – 40
309 Director, SBS
Anx.PAMB11, 1M.Sc.AMB (CMS College) 2017 2017-19 batch
Page No.69
UNIT II:
Gastric, pancreatic function and Liver function (LFT) tests.
UNIT III:
Mineral metabolism - Analysis of Sodium and Potassium electrolytes and acid base balance –
using electrolyte analyser.
UNIT IV:
Biologically important hormones: Insulin, Glucagon, Epinephrine, Thyroid, Growth
Hormone, Parathyroid, Pituitary, Steroid Hormones. Hormone analysis – TFT, pregnancy test.
UNIT V:
Characteristics of growing tumor cells – biochemical and morphological changes. Cancer
markers – AFP and CEA.
REFERENCES :
1. Tietz Fundamentals of Clinical Chemistry- Teitz, 6th Edition.
2. Practical Clinical Biochemistry -Harold Varley, 2006
3. Introduction to medical laboratory technology – F.J. Baker, R.E. Silverton, 5th
Edition, Butterworth & Co Publishers Ltd.
310 Director, SBS
CMS COLLEGE OF SCIENCE AND COMMERCE
(AUTONOMOUS)
Chinnavedampatti, Coimbatore - 641 049
An autonomous Institution, Affiliated to Bharathiar University
College with Potential Excellence (by UGC)
Re – Accredited by NAAC with ‘A’ Grade (CGPA-3.53 out of 4)
Phone No: 2666465
Email: info@cmscbe.com
Website: www.cmscbe.com
SCHOOL OF BIOLOGICAL SCIENCES
DIVISION OF MICROBIOLOGY
II Year M.Sc., Applied Microbiology
CURRICULUM, SCHEME OF EXAMINATION AND SYLLABI (CBCS) (2017)
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
311 Director, SBS
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Page No.2
REGULATIONS
MICROBIOLOGY: Activities of microorganisms are very important to almost every sector
of concern to mankind. From a perusal of the foregoing topics, one can find applications
(uses) of microorganisms to agriculture, forestry, food, industry, medicine, and environment.
The scope and significance of microbiology has enlarged manifold, particularly when
importance of environment was realized globally and the word environment was used in a
much wider sense in terms of totality to include almost everything in every bit of nature.
OBJECTIVES
After completing the Program of Study in Microbiology, students should be able to:
demonstrate an understanding of core concepts of Microbiology, including the
evolution and diversity of microbes; cell structure and function; metabolism;
information flow and the role of microbes in ecosystems;
Display proficiency in basic microbiological skills.
Analyze, interpret and evaluate a range of scientific literature in Microbiology.
Develop an information base for making personal health decisions in regard to
infectious diseases.
ELIGIBILITY
A pass in Under Graduate Examination with any Bioscience Degree or any other examination
accepted as equivalent there by the syndicate B. Sc in Biology / Botany / Zoology/ Chemistry/
Nursing / Biochemistry / Microbiology /or other examinations accepted as equivalent there to
by the Syndicate, subject to such other conditions as may be prescribed therefore.
DURATION OF PG COURSE
The course shall extend over a period of two years comprising of four semesters, with two
semesters per year (six periods – 50 minutes duration each /day). There shall not be less than
ninety instructional days during each semester. Examination shall be conducted at the end of
each semester for the respective subject.
312 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.3
DISTRIBUTION OF THE MARKS AND CREDITS UNDER CBCS
PART SUBJECT@ No of
Papers
Marks
@ Credits
I Subjects and Practicals$ 17 1700 68
II Elective Subjects 4 400 16
III Project 1 150 6
Total 22 2250 90
Note: I
@ Includes 25/40% continuous assessment marks for theory and practical subjects respectively.
$ In core subjects both theory and practical are included wherever applicable.
The following parameters are considered throughout study period
i) Regularity of Attendance
ii) Active participation in classes/Camps/Games (College/District//University)
iii) Exemplary awards/certificates/prizes
iv) Other Social Components (Blood Camp, Fine Arts, etc)
Note: II
The Credit points and Lecture Hours are not linked.
313 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.4
Annexure No: PAMB11 BOS.DT: 12.6.2017
CMS COLLEGE OF SCIENCE AND COMMERCE (AUTONOMOUS)
COIMBATORE – 641049
SCHOOL OF BIOLOGICAL SCIENCES
M.Sc., APPLIED MICROBIOLOGY DEGREE COURSE
SCHEME OF EXAMINATION- CBCS PATTERN
(FOR THE STUDENTS ADMITTED DURING THE ACADEMIC YEAR 2016)
Sem
este
r
Par
t
Su
bje
ct C
od
e
Subject Title
Inst
. H
rs p
er w
eek
Examination Details
Du
rati
on i
n
Hou
rs.
CIA
En
d S
em
Exa
m
Tot
al M
ark
s
Cre
dit
s
SEMESTER I
I
Paper-I Fundamentals of microbiology 5 3 25 75 100 4
Paper-II Microbial physiology and
metabolism 5 3 25 75 100 4
Paper-III Food and dairy microbiology 5 3 25 75 100 4
Paper-IV Molecular genetics 5 3 25 75 100 4
Practical-I
Practical-I: Microbial physiology,
Molecular genetics and Food
microbiology
5 9 40 60 100 4
Elective-I Elective I 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER II
II
Paper-V Microbial Techniques 5 3 25 75 100 4
Paper-VI Industrial Microbiology 5 3 25 75 100 4
Paper-VII Recombinant DNA Technology 5 3 25 75 100 4
314 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.5
Paper-VIII Bioethics, Biosafety, TQM and
IPR 5 3 25 75 100 4
Practical-II
Practical II: Microbial
Techniques, Industrial
Microbiology and Recombinant
DNA Technology
5 9 40 60 100 4
Elective-II Elective II 5 3 25 75 100 4
Total 30 - 165 435 600 24
SEMESTER III
III
Paper-IX Medical Microbiology 5 3 25 75 100 4
Paper-X Virology 5 3 25 75 100 4
Paper-XI Immunology and
Immunotechnology 5 3 25 75 100 4
Paper-XII Genomics and Proteomics 5 3 25 75 100 4
Elective III Elective III 5 3 25 75 100 4
Practical-III Practical III: Medical
Microbiology and Immunology 5 9 40 60 100 4
Total 30 - 165 435 600 24
SEMESTER IV
IV
Paper-XIII Environmental and Agricultural
Microbiology 5 3 25 75 100 4
Elective-IV Elective IV 5 3 25 75 100 4
Practical-IV Practical IV: Environmental and
Agricultural Microbiology 5 9 40 60 100 4
Project 15 - 100 50 150 6
Total 30 - 190 260 450 18
Grand Total - - - - 2250 90
315 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.6
Each paper carries an internal component
There is a pass minimum for an external component
Theory: Internal assessment: 25 marks; External component: 75 marks
Practical: Internal assessment: 40 marks; External component: 60 marks
PROJECT AND VIVA VOCE:
Project: 2 Reviews + Dissertation: 150 marks; Final Viva voce: 50 marks
The project report is the bonafide work carried out by the candidate under the guidance
of a faculty authenticated and countersigned by the Director. This project work must be
presented and defended by the candidate in the department attended by all faculties and
reviewed by external examiner. Candidate who has presented the work as ‘Not qualified
as per CBCS’ must resubmit the project again in the ensuing academic year.
COMPONENTS OF INTERNAL ASSESSMENT*
Theory: Two tests in each semester (one Internal Test and one Model Test). Marks from both
Tests will be taken: Total 15 marks (5 marks Internal Test and 10 marks Model Test)
Assignments and seminar: 2.5 marks + 2.5 marks = 5 marks
Overall performance: 5 marks
Practicals I, II, III and IV (for 40 marks):
Minimum 10 experiments to be conducted/ semester: 20 marks
Average of two tests: 15 marks
Record work: 05 marks
Practical: Marks should be awarded to each practical by the course teacher and the average of
the best ten practicals be taken for 40 marks.
Project: 100 marks should be awarded as internal assessment marks (80 marks for two
monthly reviews (40 + 40) conducted by the project guide and the Director, 20 marks for
Dissertation)
316 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.7
* Retest for internal examination to be conducted for genuine cases as per the
recommendations of class in charge, subject in charge. The final decision to be made by the
Director.
Electives: List of Group Elective papers (Students can choose any one of the Group papers as
electives)
Papers Group A Group B Group C
Paper I / Sem I Research
Methodology
Environment and
Health
Basic biological
sciences
Paper II / Sem II Bioinformatics
Communicable and
non-communicable
disease
Biophysics and
Biochemistry
Paper III / Sem III Pharmaceutical
Microbiology
Health care of the
community
Molecular cytology and
tissue Engineering
Paper IV / Sem IV
Diagnostic
Microbiology and
Molecular
Diagnostics
Quality assessments in
pharmaceuticals
Marine Biology and
Aquaculture
317 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.8
Annex EDC: PG BS
BOS DT: 02:06:2017
CMS COLLEGE OF SCIENCE & COMMERCE, (AUTONOMOUS)
COIMBATORE – 641 049.
M. Sc. Biotechnology/Biochemistry/Microbiology/Applied Microbiology
EXTRA DISCIPLINARY COURSE (EDC)
(For the students admitted during the academic year 2015 onwards)
Note:
The student has to compulsorily select one Extra Disciplinary Course offered by other
departments and is eligible to get 4 extra credits. This paper is offered as self study from the
2015 batch onwards.
Par
t
Su
b c
ode
Subject
Ins.
hrs
/ w
eek
Examinations
Du
r.
Exa
m
in
hrs
CIA
ES
E
Tot
al
mar
ks
Cre
dit
Semester III
Organic farming - 3 25 75 100 4
Total - - - - 100 4
318 Director, SBS
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Page No.9
COURSE: EXTRA DISCIPLINARY COURSE
SUBJECT TITLE: Organic Farming
NUMBER OF HOURS/WEEK: Nil
SUBJECT DESCRIPTION
This course deals with the principles and practices of organic farming for sustainable
development.
GOALS
To make the student to understand basics, importance and advantages of organic farming.
OBJECTIVES
On successful completion of subject student would know about Organic farming,
vermicomposting and their applications.
CONTENTS
UNIT-I
Organic farming- Concept - Principles - Importance- Types- Its relevance to Indian agriculture
- Soil fertility - Nutrient recycling - Crop rotations - Requirements for organic farming -
Advantages and applications of organic farming.
UNIT-II
Methods of organic farming - Land preparation and mulching - Water management-green
manure - Composting methods - Vermicomposting – Different types of earthworms -
Different methods for vermi composting – Vermiwash - Advantages and applications - Value
addition as biofertilizer and marketing strategies.
UNIT-III
Organic farming for sustainable agriculture and crop production - Importance and different
types of biofertilizers - Production of Azolla, Azospirillum, Azotobacter and Rhizobium
biofertilizers- Ecofriendly applications of biofertilizers.
CIA – 25
ESE – 75
319 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.10
UNIT-IV
Disease and pest management in organic farming - Biological pest control- Bio pesticides -
Neem insecticides - Integrated pest management - Bt insecticides - Advantages and
applications – Control of weeds.
UNIT-V
Integrated farming system - Objectives- Components - Factors affecting ecological balance-
Inspection, Certification and accreditation of organic product - Marketing and export potential
- Organic farming and national economy.
REFERENCES:
1. Hand Book of Organic Farming. Sharma A. 2002. Agrobios.
2. Organic Farming. Lampin N. 1990. Press Books, Lpswitch, UK.
3. Organic Farming and Sustainable Agriculture. Veeresh GK, Shivashankar K and
Suiglachar MA. 1997.
4. Organic Farming – Theory and Practice. Palaniappan SP and Anandurai K. 1999.
Scientific Publishers.
320 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.11
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper IX: Medical Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
Medical microbiology is a branch of medicine concerned with the prevention, diagnosis and
treatment of infectious diseases. In addition, this field of science studies various clinical
applications of microbes for the improvement of health.
GOALS
To gain knowledge on the aspects of the inter relationship between infectious agents and their
hosts.
OBJECTIVES
On successful completion of the subject, the student will know about the mechanism of
infection and its types & provides the information about the prevention of diseases.
CONTENTS
UNIT-I
Introduction to Medical Microbiology: Role of Microorganisms in medicine, classification
of Medically Important microbes - Normal microflora. Infections: sources and mode of
transmission, infectious disease cycle, epidemic, endemic, pandemic diseases. Antibiotic and
chemotherapeutic agents- Mode of action. Antibiotic resistance – ABST and CLSI guidelines.
UNIT-II
Medical Bacteriology: Morphology, cultural characteristics, pathogenicity, laboratory
diagnosis and treatment of: Staphylococcus aureus, Streptococcus pneumonia, Bacillus
anthracis, Corynebacterium diphtheriae, Clostridium tetani, Mycobacterium tuberculosis and
M. leprae, E.coli, Salmonella typhi, Shigella dysentriae, Klebsiella pneumoniae, Proteus
vulgaris, Vibrio cholerae, Pseudomonas aeruginosa and Neisseria gonorrheae.
CIA – 25
ESE – 75
321 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.12
UNIT-III
Medical Mycology: Pathogenicity, laboratory diagnosis and treatment of superficial mycoses
(Tinea, Piedra), cutaneous mycoses – Dermatophytoses, Candidosis, Subcutaneous mycoses–
(Sporotrichosis, Mycetoma), Systemic mycosis (Cryptococcosis, Histoplasmosis).
UNIT-IV
Medical parasitology: Morphology and Identification of parasites. Pathogenicity, laboratory
diagnosis and treatment of Protozoan infection of Entamoeba histolytica, Giardia intestinalis,
Trypanosoma cruzi, Plasmodium falciparum, Toxoplasma gondii, Taeniasaginata,
Ancylostoma duodenale, Enterobius vemiculari, Ascaris lumbricoides and Wuchereria
bancrofti.
UNIT-V
Sytemic Infection: UTI, URTI, LRTI, STD, Skin infections, Nosocomial, PUO– collection
transport and processing of samples. Molecular diagnostics of Microbial infections: TB,
Malaria, Aspergillosis and toxoplasmosis.
TEXT BOOKS:
1. Textbook of Microbiology– Ananthanarayanan, R. and JayaramPanicker, C.K. (2005)
7th ed.Orient Longman Pvt., Ltd.
2. Textbook of Medical parasitology- Subash Chandra Parija, 2nd ed. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, Melnick and Adelberg’s (2001)22nd ed. Mc Graw
Hill Medical Publication Division.
2. Medical Microbiology - David Greenwood. Richard Slack and John Peutherer (2000)
15th ed. Church Hill Living stone Publication.
3. Medical Parasitology – Chatterjee K.D. (2007)7thed.
4. Medical Microbiology - Patric R. Murray (1990) Mosby Publications.
5. Textbook of Medical parasitology – Jayaram Panicker C.K. (2004) JP Brothers,
Medical publishers, New Delhi.
322 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.13
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Core Paper XII: Virology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of virus related to bacteria, plants and animals. It provides
knowledge about the viral morphology, replication, epidemiology, pathogenesis and
prophylaxsis of DNA and RNA viruses.
GOAL
This paper, virology has been designed to provide the student with a firm foundation in the
aspects of role of viruses in Microbiology, which forms a base for their future research. This
paper also provides the basic information about different bacterial, plant and animal viruses
including oncogenic viruses.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding
about the different form viruses related to bacteria, plant and animals. Viral life cycle
including infection manifestation, multiplication, clinical aspects and prophylaxis has been
understood.
CONTENTS
UNIT-I
Viruses - Discovery of viruses, virus taxonomy and classification, origin and properties of
viruses, multiplication of viruses and its multiplication cycle. Structure of virus particles –
filamentous viruses, isometric viruses and enveloped viruses.
UNIT-II
Viral infection - Viral attachment and entry of genome into target cell, infection of viruses in
bacterial cells, animal cells and plant cells. Viral DNA replication - linear ss DNA and linear
CIA – 25
ESE – 75
323 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.14
ds DNA, circular ss DNA and circular ds DNA. Viral RNA replication - nature and diversity
of RNA virus genomes, regulatory elements for RNA viral genome synthesis, RNA
replication with DNA intermediate.
UNIT-III
Bacterial virology - Classification and characteristics of bacteriophages, topography of
bacteriophages and their components, the virus infection cycle, plaque assay, T-series of DNA
bacteriophages - temperate, small DNA, DNA, RNA bacteriophages, bacteriophage growth
curve, replication of T-even phages, transfer of bacterial genes by transduction, phage
conversion.
UNIT-IV
Plant virology - Properties and morphology of plant viruses, transmission of plant viruses,
disease symptoms of plant virus, structure and morphology, mode of transmission, replication,
diagnostics and prevention of RTMV, CaMV, Rice stripe virus, Sugarcane mosaic potyvirus,
Cotton leaf crumple Bigemini virus, Potato virus X, Tomato yellow mosaic virus.
UNIT-V
Animal virology - Classification, structure, genome, replication, clinical aspects, laboratory
diagnosis and treatment and control of - DNA viruses: Pox viruses, Herpes simplex virus,
Adenovirus, Hepatitis B virus, Retrovirus. RNA viruses: Influenza virus, Rhabdo virus, Flavi
virus, HIV, Picorna virus, Reovirus.
TEXT BOOKS:
1. Virology – Saravanan .P (2009) MJP Publishers.
2. Introduction to modern virology - Dimmok and Primrose, S.B. (1994) 4th Ed.
BlackwellScientific Company Publications.
REFERENCE BOOKS:
1. Textbook of Microbiology – Ananthanarayanan, R and Jayaram Panicker, C.K.
(2005) 7thEd. Orient Longman, Pvt. Ltd.
324 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.15
2. An Introduction to viruses - Biswass,S.B. and Amita Biswass (1984) 3rd Ed. Vikas
Publishing House Pvt. Ltd.
3. Medical Microbiology - Jawetz, E and Melnic, J.L. (2001) Tata Mc Graw Publishing
House, New Delhi.
4. Diagnostic Microbiology- Baron E.J, Peterson L.R. and Finegold, S.M.(1994)
BaileyN. Scotts, 9thEd.Mosby Publications.
5. Virology - Lewy, J.A., Fraenled, H.C. and Owens, R.A. (1994) 3rdEd. Prentice Hall.
325 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.16
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper XI – Immunology and Immunotechnology
NUMBER OF HOURS/WEEK: 5 hours
SUBJECT DESCRIPTION
This subject describes the defense mechanisms of the higher vertebrates against invading
pathogens, tumor immunology and autoimmunity.
GOALS
To understand the basics of human defense mechanisms against the invading foreign bodies.
OBJECTIVES
On successful completion of the subject, the student will be aware of immune system,
immunity and basic immunological techniques and their applications.
CONTENTS
UNIT-I
Introduction to Immunology, History and scope of Immunology – Cells of immune system –
Hematopoiesis and differentiation – Structure and functions of Lymphocytes – T cells (TH
cells, TC cells, TS cells), B cells and NK cells, Monocytes and Macrophages, Neutrophils,
Eosinophils, Basophils and Mast cells, Organization and structure of lymphoid organs –
Primary lymphoid organs – Bone marrow, Bursa of Fabricius and Thymus. Secondary
lymphoid tissues – Spleen, lymph nodes, MALT, BALT & GALT.
UNIT-II
Primary and Secondary immune response. Immunity – Innate immunity – Different barriers
involved in innate immunity – Acquired immunity – Humoral and cell mediated Immunity.
Antigens – Factors influencing immunogenicity, Adjuvants, Epitopes, Haptens, Super
antigens and mitogens. Antibodies – Structure and functions of different immunoglobulin
classes, Production and applications of monoclonal and polyclonal antibodies. Definition and
CIA – 25
ESE – 75
326 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.17
Classification of Exo and Endo toxins. Vaccines: Active and Passive immunization, Types of
vaccines: Killed, attenuated and recombinant vaccines.
UNIT-III
Major Histocompatibility Complex (MHC): types – Class I, II and III – distribution and
function – Antigen recognition and presentation – Activation of B and T Lymphocytes –
Cytokines and their role in immune recognition – Structure, types and biology of complement
system – Hypersensitivity reactions – Type I, II, III and IV. Autoimmune disorders – Organ
specific autoimmune diseases – Hashimoto’s thyroiditis & Systemic autoimmune diseases –
SLE and AIDS.
UNIT-IV
Tumor immunology – tumor antigens – immune response to tumors – Cancer immunotherapy.
Transplantation immunology (Graft vs Host reaction (GVHR), Host vs Graft reaction
(HVGR), MLR, HLA typing – Organ transplantation (Bone marrow transplantation, Kidney
transplantation, heart and liver transplantation) – Immunosuppressants and
immunosuppressive therapy.
UNIT-V
Immunotechnology: Salient features of antigen – antibody interactions, In vitro tests –
Precipitation reactions: Precipitation reaction in fluids, Precipitation reaction in gels: Radial,
immunodiffusion, Double immunodiffusion, Immunoelectrophoresis. Agglutination reactions:
Haemagglutination, Bacterial and Passive agglutination. ABO blood grouping, Coomb’s test,
Widal, VDRL, ASO, CRP, ELISA and complement fixation test. Immunofluorescent
techniques – FISH, FACS and immuno electron microscopy. Immunohistochemistry – An
Overview.
TEXT BOOKS:
1. Kuby Immunology. Thomas J. Kindt, Richard A. Goldsby, Barbara A. Osborne
(2007) 6th edition, W.H. Freeman & Company, New York.
327 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.18
REFERENCE BOOKS:
1. Essentials of Immunology. Ivan Riot (1988), 6th edition, Blackwell Scientific
Publications, Oxford.
2. Fundamentals of Immunology. Paul W.E. (1988) Raven Press, New York
3. Immunology – Jan Klein and Vaclav Horejsi (1999) Wiley-Blackwell, New York
4. Janeway’s Immunobiology – Kenneth Murphy and Casey Weaver – 9th Edison,
Garland Science, New York.
328 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.19
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper XII: Genomics and Proteomics
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper deals with genome map, comparative genomics, structural genomics, functional
genomics, protein structure prediction, function and various tools for analysis of proteins.
GOALS
To make the students familiar with genome map, comparative genomics, structural and
functional genomics and proteomics - extensively used in drug discovery and in learning
various tools for analysis of proteins.
OBJECTIVES
To understand the genome architecture and extracting information like gene function, gene
regulation, protein evolution and targets for drug designing.
CONTENTS
UNIT-I
Genomics: Introduction to Genomics, Genome topology: Chromatin, super coiling and
packaging. Genome organization: Genome organization in prokaryotic and eukaryotic
systems: Operon concept. Genome sequencing - Shot gun, clone-contings, pyrosequening.
NGS (Next Generation Sequencing).
Genome analysis - Chromosome analysis: Basic strategy for genetic analysis in human:
Linkage mapping, physical mapping, genetic mapping and restriction mapping.
UNIT-II
Annotation of the Genome: Various approaches in gene prediction, ORF prediction, Gene
prediction in prokaryotes, Gene prediction in eukaryotes, Hidden Markov Model, Pattern
CIA – 25
ESE – 75
329 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.20
discrimination, Evaluation of gene prediction method and Prediction of promoter sequences.
Applications of Genomics
UNIT-III
Functional Genomics: Gene expression analysis by cDNA micro arrays, SAGE. EST
databases (DBEST, UNIGENE).
Genomic Diversity- General Purpose of Comparative Genomics Database: Cog- Cluster of
Orthologous Groups, Kegg Kyoto Encyclopedia of Genes and Genomes, MBGD Microbial
Genome Database, Tools for Genomic Comparison and functions of functional genomics.
UNIT-IV
Principles of Protein classification: Based on Structural features, Phylogenetic relationship,
CATH - Classification by Class, Architecture, Topology, Homology, SCOP - Structural
Classification of Protein, FSSP – Fold classification based on structure - structure alignment,
MMDB - Molecular Modeling Database. Secondary structure prediction: Chou – Fasman /
GOR method, Neural network.
UNIT-V
Analytical proteomics: Sample preparation and processing, Proteome analysis techniques:
2D PAGE, Capillary Electrophoresis, Spectroscopy: NMR, MS and MALDI-TOF and its
variants. 3D structural analysis: X-ray crystallography / X-ray diffraction analysis.
Applications of proteomics.
TEXT BOOKS:
1. Bioinformatics - Methods and Applications, Genomics, Proteomics & Drug
discovery –S.C. Rastogi, Namita Mendiratta & Parag Rastogi. Printice Hall of India
private limited, 4th ed., 2013, New Delhi.
2. Principles of Genome analysis and genomics – S.B. Primrose and Twyman (2003)
3rd Edition, Blackwell publishing.
3. Bioinformatics - Sequence and Genome analysis- David W Mount, CBS publishers
and distributors, 2nd ed., 2005.
330 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.21
4. Bioinformatics - A practical guide to the analysis of genes and proteins- Andreas
D Baxevanis & B F Francis Ouellette , A John Wiley and Sons, INC., Publications, 2nd
ed., 2002.
5. Genomics – The science and technology behind the human genome project –
Charles R. Cantor, 4th ed., 1999.
6. Proteomics. S.R. Pennigton and M.J. Dunn. 2002. 2nd., Viva Books Private Limited.
New Delhi (for Units III and IV and V.)
REFERENCE BOOKS:
1. Proteomics- From protein sequence to function - S R Pennington & M J Dunn (2002),
2nd ed., Viva Books Pvt. Ltd.
2. Genes VII - Lewin B (2000), 7th ed., Oxford University Press, UK.
3. Essential Bioinformatics - Jin Xiong (2006), Cambridge University Press.
4. Recent advances in Bioinformatics - Irfan A Khan & Atiya Khunum (2003), Ukaaz
Publications.
5. Introduction to Proteomics – Principles and applications by Nawin Mishra, 2010.
6. Proteomics in practice – Reiner Westermeier and Tom Naven, 2002.
331 Director, SBS
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Page No.22
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective III: Group A- Pharmaceutical Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of enzyme and antimicrobial products production related to
pharmacy. The information about gene therapy and diagnostic aids indicates the usage of
advancement in pharmacy research. Concepts of intellectual property rights and international
trade in the syllabus infer importance of patenting and trading of pharmaceutical products.
GOAL
This paper in microbiology has been designed to provide the student with a firm foundation in
the pharmaceutical aspects of microbiology, which forms a base for their future research. This
paper also provides the basics in IPR for better understanding of industrialization of the
pharmaceutical products.
OBJECTIVE
On the successful completion of the course, the students will get an overall understanding of
production aspects of pharmaceutical important enzymes and products, diagnostic aids and
intellectual property and international trade.
CONTENTS
UNIT-I
Introduction to Pharmaceutical microbiology - The ecology of microorganisms affecting
pharmaceutical industry – The atmosphere, water, skin & respiratory flora of personnel, raw-
materials, packing, equipments, building, utensils etc. Types of microorganisms occurring in
pharmaceutical products. Types of antimicrobial agents and their uses (alcohol, phenolic
compounds, formaldehyde).
CIA – 25
ESE – 75
332 Director, SBS
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UNIT-II
Antimicrobial agents: Types of Antibacterial, antifungal and antiviral compounds.
Commercial production and mode of action - penicillin, cephalosporin.
UNIT-III
Assay for growth inhibiting substances – Assay for non-medicinal antimicrobials (Phenol
coefficient/Rideal-Walker Coefficient). Drug sensitivity testing methods and their importance.
Assay for antibiotics – Determination of MIC, the liquid tube assay, solid agar tube assay,
agar plate assay (disc diffusion, agar well and cylinders cup method).
UNIT-IV
Gene therapy and diagnostic aids: Disease prevention by vaccines (DNA vaccines), disease
diagnosis probes, monoclonal antibodies, disease treatment products from recombinant
organisms, interferon, antisense nucleotides as therapeutic agents, drug delivery (Viral
delivery and therapeutic strategies, non-viral delivery, gene delivery to skin, use of liposomes
as drug delivery system).
UNIT-V
Intellectual Property: Concepts regarding Intellectual Property (IP) Intellectual Property
Protection (IPP) and Intellectual Property rights (IPR). Economic Importance mechanisms for
protection of Intellectual Property - patents, copy rights, trademarks, Factors affecting choice
of IP Protection penalties for violation, Role of IP in Pharma industry: Global ramifications
and financial implications.
TEXT BOOKS
1. Pharmaceutical biotechnology – Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. andRussel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS
1. Gene therapy - Templeton and Lasic (2000) Marcel and Dekkn.
333 Director, SBS
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Page No.24
2. The basic science of gene therapy – Richard C. Mulligan (1993) American
Association for the Advancement of Science.
3. Comprehensive biotechnology - Murray Moo-Young (Ed) Vol-3 (2004) Permagon
Press.
4. Pharmaceutical biotechnology - Purohit (2003) 2nded. Agrobios.
334 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.25
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective III: Group B – Health Care of the Community
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
The paper describes about the contact between the individual and the health system.
GOALS
To gain knowledge about the types of health care system provided in the society.
OBJECTIVES
On successful completion of the subject, the student will know about the personal health care,
physical and mental health.
CONTENTS
UNIT-I
Nutrition and health - Balanced Diet, food surveillance, food fortifications- addition of
vitamins and minerals - Adulteration and preventive steps.
UNIT-II
Physical health-care of skin, hair, teeth, eyes, ears, hands and feet-physical exercises and their
importance - Walking and jogging – Yoga and meditation – stress Relief.
UNIT-III
Health Programmes and health education – Malaria control – TB control – AIDS control
programmes Immunization programmes.
CIA – 25
ESE – 75
335 Director, SBS
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UNIT-IV
Social sciences and mental health – Sociology; Social structure, culture and customs – social
problems-Mental health – cases of mental illness Alcoholism and drug dependence –
prevention Rehabilitation.
UNIT-V
Family planning, Maternal and child health – Antenatal and Postnatal care – Reproductive and
child Health programme. (RCH).
REFERENCES:
1. Park’s Text books of preventive and social medicine
2. Immune-biotechnology by Naha & Narain
3. Immunology by Dulsy Fatima & N.Arumugam
4. Food and Nutrition by L.Swaminathan
5. Dietics by Srilakshmi
6. Practice of fertility control and Comprehensive manual - S.K.Choudhary. 6th
edition.
336 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.27
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective III: Group C – Molecular Cytology and Tissue Engineering
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
Molecular Cytology and tissue engineering deals with the study of the structure and functions
of the cells of animals and plants; especially, the study of the cells of the human body, and of
the significance of changes
GOAL
To provide the knowledge of tissue engineering in the use of a combination
of cells, engineering and materials methods, and suitable biochemical and physicochemical
factors to improve or replace biological functions.
OBJECTIVE
Understanding the principles of basic cell molecular biology and the fundamental and
quantitative principles of tissue engineering.
CONTENTS
UNIT-I
Molecular basis of signal transduction in bacteria, plants and animals; Model membranes;
protein sorting, secretory and endocytic pathways, cell cycle; Dosage compensation and sex
determination and sex-linked inheritance
UNIT-II
The law of DNA constancy and C-value paradox; Numerical, and structural changes in
chromosomes; Molecular basis of spontaneous and induced mutations and their role in
evolution; polypoidy; Environmental mutagenesis and toxicity testing; Population genetics.
Environmental regulation of gene expression
CIA – 25
ESE – 75
337 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
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UNIT-III
Cell and tissue culture in plants and animals; Primary culture; Cell line; Cell clones; Callus
cultures; Somaclonal variation; Micropropagation; Somatic embryogenesis; Haploidy;
Protoplast fusion and somatic hybridization; Cybrids.
UNIT-IV
Gene transfer methods in plants and in animals; Transgenic biology; Allopheny; Artificial
seeds; Gene targeting. Applications of genetic engineering in agriculture, health and industry.
UNIT-V
Histology –Basics of mammalian systems, nutrition, digestion and absorption; Circulation
(open and closed circular, lymphatic systems, blood composition and function); Excretion and
osmoregulation: Homeostatis (neural and hormonal); Bioluminiscence.
TEXT BOOKS:
1. Molecular Biology of the Cell. Alberts, B. et al. (2008). 5th Ed. Garland Publishing
House.
2. The World of the Cell. Becker. (2009). 7th ed. Benjamin-Cummings.
3. Genomes 2. Brown, T. A. (2002). Wilely-Liss.
REFERENCE BOOKS:
1. The Cell. Cooper, G. M. (2004). 3rd ed. ASM Press.
2. Introduction to genetic analysis. Griffiths, A. J. F., Wessler, S. R., Lewontin, R. C.
and Carroll, S. B. 2008. 9th ed. W. H. Freeman and Company, New York.
3. Modern Genetic Analysis: Integrating Genes and Genomics. Griffiths, A. J. F.
(2002). 2nd ed. W. H. Freeman and Company, New York.
4. Tissue Engineering - Clemens Van Blitterswick (2008). Academic Press 1st edition
338 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.29
SEMESTER – III
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Practical III: Medical Microbiology and Immunology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This Practical paper deals with the pathogenic microorganism of human beings, serology and
immunological techniques.
GOAL
This paper gives strong basic knowledge in sample collection and identification of various
pathogenic microorganisms.
OBJECTIVE
On the successful completion of the course, the students will get an overall knowledge in
medical microbiology.
CONTENTS
Medical Microbiology:
1. Collection, transport and processing of specimens – Urine, sputum, pus, blood, throat
swab, stools and wound exudates.
2. Isolation and identification of specimen associated bacterial pathogens - Escherichia
coli, Klebsiella sp., Proteus sp., Salmonella sp., Shigella sp., Pseudomonas sp.,
Staphylococcus sp., Streptococcus sp.
3. Antibiotic sensitivity pattern of clinical isolates.
4. Cultivation of animal virus in embryonated eggs (Demonstration)
Serology:
A. Agglutination Reactions
5. ABO blood grouping
CIA – 40
ESE – 60
339 Director, SBS
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Page No.30
6. Antistreptolysin- O test
7. C-reactive protein test
8. WIDAL test (qualitative and quantitative)
B. Precipitation Reactions
9. Counter-current immunoelectrophoresis
10. Immunoelectrophoresis
11. Ouchterlony’s double immunodiffusion test
12. Radial immunodiffusion test
13. Dot-ELISA test
REFERENCES:
1. Experiments in microbiology plant pathology and biotechnology – Aneja, K.R.
(2003) 4thed. New age international publ.
2. Microbiology – Laboratory Manual - James G. Cappuccino and Natalie Sherman
(1999) 6th ed. Benjamin/Cummings Science Publishing.
3. Microbiology Manual – Kannan, N, (2000) Palani Paramount Publishing.
4. Microbiology-Laboratory Manual – Sudarajan, M. (2001)
5. Experimental Procedures in Life Sciences - Rajan, S. (2010) Anjana book house.
340 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.31
SEMESTER – IV
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Paper-XIII-Environmental and Agricultural Microbiology
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of soil, microbial inhabitants and its association with plant and
other organisms.
GOAL
This paper is designed to give basic knowledge about types and structure of the soil, both
beneficial and harmful microorganisms present in the soil.
OBJECTIVE
After the completion of this paper, the Students will understand the interaction of
microorganisms with the plants and the production of bio fertilizers.
CONTENTS
UNIT-I
Soil and Microorganisms: Physical and chemical characteristics of soil, Soil microflora-
bacteria, actinomycetes, algae, fungi, virus, protozoans & nematodes and their significance.
Microbial interactions with plants: Phyllosphere and Rhizosphere, Microbial interactions with
animals - insect and rumen, Brief account on microbial interactions - symbiosis, mutualism,
commensalism, competition, amensalism, synergism, parasitism, predation.
UNIT-II
Pollution Types: Air-pollution, sources, effects, Biological treatment of toxic gases-aerosol,
droplet nuclei- air quality analysis-air sampling device-air borne diseases. Water-sources
(microbiological)-water quality analysis, MPN technique and Membrane filtration, biological
treatment methods (aerobic, anaerobic methods) for effluent, Water borne diseases-Typhoid,
CIA – 25
ESE – 75
341 Director, SBS
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cholera, Amoebic dysentery. Microbial indicators of pollution. BOD and COD, Solid waste
management methods.
UNIT-III
Biogeochemical cycles: Mobilization and immobilization of elements - carbon cycle –
nitrogen cycle. Biological nitrogen fixation - nitrogenase enzyme, nif genes, symbiotic
nitrogen fixation (Rhizobium, Frankia), non-symbiotic nitrogen fixation (Azotobacter sp and
Azospirillum sp), VAM – ecto, endo & ectendomycorrhizae - phosphorous, sulfur and Iron
cycle. Biodegradation of xenobiotics, Biosorption and Biomining.
UNIT-IV
Biofertilizers and Biopesticides: Biofertilizers and their importance in crop productivity,
Mass production of bacterial biofertilizers (Rhizobium, Azospirillum, Azotobacterand
Phosphate solubilizing bacteria), Algal, Cyanobacterial and fungal (Mycorrhizae)
biofertilizers - significance and practice, Biopesticides: Bacterial (Bt pesticides), fungal
(Trichoderma), Viral biopesticides – Baculovirus, NPV insecticides, Biomass production,
formulation and large scale application.
UNIT-V
Plant pathology: Symptoms, disease cycle and control measures of Bacterial diseases -
Blight of rice, citrus canker, wilt of potato, fungal diseases - Blast of rice, rust of wheat, smut
of sugarcane, red rot of sugarcane and viral diseases - Vein clearing disease, TMV.
Transmission of Viral infections through vectors - grasshopper and aphids.
TEXT BOOKS:
1. Soil Microbiology – Subba Rao, N.S. (2004) 4th edition, Oxford & IBH Publishing
Co.pvt. Ltd. New Delhi.
2. Soil Microbiology - Mishra, R.R. (2004) 1st edition, CBS Publishers and distributors,
New Delhi.
3. Diseases of Crop Plants in India - Rangaswamy, G &Mahadevan, A. (2002) 4th
edition, Prentice-Half of India Pvt Ltd, New Delhi.
4. Biofertilizers in Agriculture and Forestry - Subbarao, N.S. (1995) 3rd edition,
Oxford Ltd, New Delhi.
342 Director, SBS
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REFERENCE BOOKS:
1. Agricultural Microbiology - Rangaswami, G & D.G. Bagyaraj, 2nd Edition, Prentice-
Hall of India, New Delhi.
2. Microbial Ecology - Atlas R.N &Bartha, R. 4th edition, Benjamin-Cummings.
3. Soil Microbiology - Robert, L. Tate, 1st edition, John Wiley & Sons, Inc. Newyork.
4. Introduction to Soil Microbiology - Alexander, M. John Wiley & Sons, Inc.
Newyork.
343 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.34
SEMESTER – IV
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Elective IV: Group: A – Diagnostic Microbiology and Molecular
Diagnosis
NUMBER OF HOURS/WEEK: 5 Hrs
SUBJECT DESCRIPTION
This paper presents the study of diagnosis of various infectious agents.
GOALS
This paper has been designed to understand the basics of medically important microorganisms
and to diagnosis of a pathogen through safe handling and processing techniques
OBJECTIVES
On the successful completion of the course, the students will have an overall understanding of
various techniques required to isolate the given pathogen.
CONTENTS
UNIT-I
General concepts in specimen collection and handling in bacterial infections: Basic principles
of specimen Collection – Preservation, storage, transport of specimen (urine, stool, pus and
blood) and processing of clinical samples – Direct examination – Microscopy – Culture
methods for bacteria.
UNIT-II
Emerging technologies in detection of bacterial infections: Microscopic methods for rapid
detection – (Vitek, Microscan, Sensititre and Pasco methods). Direct microbial antigenic
detection – Precipitation test (Immunodiffusion test), agglutination test (Latex),
Immunofluorescence assay, Enzyme immunoassay (ELISA). Serological diagnosis –
complement fixation. Neutralization test (ASO test), Western blot technique. Molecular
Methods – Nucleic acid hybridization and PCR.
CIA – 25
ESE – 75
344 Director, SBS
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UNIT-III
Lab diagnosis of viral infections: Specimen selection and collection – throat, naspharyngeal
swabs, bronchial washes, rectal swabs, stool and skin lesions. Virus detection methods –
cytology and histology, Electron microscopy, Immunodiagnosis – antigen detection, PCR and
cell culture (cell line and cytopathic effect).
UNIT-IV
Lab diagnosis of fungal infections: collection and transport of clinical specimens – respiratory
tract secretions, CSF, blood and urine. Identification methods – direct detection, microscopy
and cultivation of dermatophytes. Opportunistic mycoses – Aspergillus spp, Systemic
mycoses – Histoplasma capsulatum and Candida albicans
UNIT-V
Lab diagnosis of parasitic infections: General specimen collection and transport of specimens
– Approaches in identification – O&P examination – pinworm examination – Biopsy
examination – blood smear staining (thick and thin). Cultivation – Larval stages, nematodes
and protozoa. Organism identification in body organs – amoeba and flagellates.
TEXT BOOKS:
1. Diagnostic Microbiology (Bailey& Scott’s) - Betty A Forbes and Daniel Sahm, 12th
edition, Mosby Elsevier publications.
2. Textbook of Microbiology– Ananthanarayanan, R. and Jayaram Panicker, C.K.
(2005). 7th ed. Orient Longman Pvt., Ltd.
3. Textbook of Medical parasitology- Subash Chandra Parija, 2nd ed. All India
Publishers & Distributors.
REFERENCE BOOKS:
1. Medical Microbiology – Jawetz, Melnick and Adelberg’s (2001). 22nd ed. McGraw
Hill Medical Publication Division.
2. Medical Microbiology - David Greenwood. Richard Slack and John Peutherer.
(2000). 15th ed. Church Hill Living stone Publication.
345 Director, SBS
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Page No.36
3. Medical Parasitology – Chatterjee K.D. (2007) 7th ed.
4. Medical Microbiology - Patric R. Murray (1990) Mosby Publications.
5. Textbook of Medical parasitology– Jayaram Panicker C.K. (2004). JP Brothers,
Medical publishers, New Delhi.
346 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.37
SEMESTER – IV
COURSE: M.Sc: Applied Microbiology
PAPER TITLE: Elective-IV: Group B – Quality Assessment In Pharmaceuticals
NUMBER OF HOURS/WEEK: 5 hrs
SUBJECT DESCRIPTION
This paper presents the study of various aspects involved in product quality and quality
assurance, maintenance of quality with good manufacturing practices.
GOALS
This paper has been designed to understand the principles of quality assurance as a function of
total quality management and the methodology necessary to establish and implement quality
assurance program for a manufacturing plant.
OBJECTIVES
On the successful completion of the course, the students will have an overall understanding of
a comprehensive review of quality assurance, from the concepts and practical applications of
total quality management to all aspects of the manufacturing procedures. The information also
provides students with a broad foundation in this area of pharmaceutical industries.
CONTENTS
UNIT-I
An introduction to pharmaceutical microbiology. Chemical growth control. Chemical
antimicrobial agents for external use, synthetic antimicrobial drugs, naturally occurring
antimicrobial drugs: Antibiotics. Antibiotics from prokaryotes, antiviral drugs, antifungal
drugs, antimicrobial drug resistance, the search for new antimicrobial drugs.
UNIT-II
Types of spoilage, Factors affecting microbial spoilage – assessment of microbial spoilage –
preservation. Ecology of microorganisms as it affects the pharmaceutical industry – Sterile
CIA – 25
ESE – 75
347 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.38
pharmaceutical products – injections, Non injectable sterile fluids, Ophthalmic preparations,
dressings & implants.
UNIT-III
Sterilization control - methods of sterility testing- sterilization monitors and Quality assurance
of products. The microbiological quality and regulatory requirements for natural and
nutraceutical products, The regulatory control and quality assurance of immunological
products, Containment system integrity – sterile products, Regulatory guidelines
(microbiology) for veterinary antimicrobial products.
UNIT-IV
The role of the Qualified Person in microbiological quality assurance, Safety in microbiology,
Rapid enumeration and identification methods, Selection and use of cleaning and disinfection
agents in pharmaceutical manufacturing, Prevention and elimination of microbial biofilms in
the manufacturing environment using Clean-in-Place, Cleanroom design, operation and
regulatory standards.
UNIT-V
Microbiological quality assurance. Validation of aseptic processing and media fills,
International disinfectant testing protocols, Measurement of biocide effectiveness,
Microbiological quality and regulatory requirements for biotherapeutics and manufactured
products, The role of the microbiologist in HACCP, Auditing the pharmaceutical
microbiology department.
TEXT BOOKS:
1. Pharmaceutical biotechnology - Purohit, Kulkarni, Saluja (2003) Agrobios.
2. Pharmaceutical Microbiology – Hugo, WB. And Russel, AD. Blackwell Scientific,
Oxford.
REFERENCE BOOKS:
1. Gene therapy - Templeton and Lasic. (2000). Marcel and Dekkn.
348 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.39
2. The basic science of gene therapy – Richard C. Mulligan. (1993). American
Association for the Advancement of Science.
3. Comprehensive biotechnology - Murray Moo-Young (Ed) Vol-3. (2004). Permagon
Press.
4. Pharmaceutical biotechnology – Purohit. (2003). 2nded. Agrobios.
349 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.40
SEMESTER – IV
COURSE: M. Sc., Applied Microbiology
PAPER TITLE: Elective-IV: Group C – Marine Biology and Aquaculture
NUMBER OF HOURS/WEEK: 5 hrs
SUBJECT DESCRIPTION
This paper deals with the aquatic environment and the types of biological community present
in it and adds on the fisheries management and its pathology.
GOAL
The completion of this paper provides the students to get are of the marine environment and to
create basic understanding of the nutritional requirements of fish/shellfish.
OBJECTIVE
To provide a deep knowledge on the marine biology and aquaculture.
CONTENTS
UNIT-I
Biological Oceanography: The sea as a Biological environment: classification of marine
environment; general characters of the populations of the primary biotic divisions (plankton,
nekton and benthos) - Introduction of plankton; general classification and composition of
plankton; floating mechanism of plankton. Collection of plankton; general account of
instruments and nets employed; methods of fixation and preservation of plankton; analysis of
samples, methods of estimating standing crop of plankton.
UNIT-II
Biology of Marine Organisms-I: Nutrition: Types of food, general mechanisms of feeding;
transport of food through gut: digestion, digestive enzymes. Excretion – Nitrogen excretion;
mode of nitrogen excretion, Elimination of nitrogenous waste. Respiration respiratory
mechanisms: factors effecting respiration (Salinity, temperature and oxygen tension);
CIA – 25
ESE – 75
350 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.41
respiratory pigments: role in transport of oxygen and carbon dioxide. Osmotic regulation and
ion regulation: mechanisms: general account.
UNIT-III
Estuaries and Coastal Zone management: Estuarine environment: classification and
physico – chemical parameters of estuaries. Distribution of estuarine plankton, nekton and
benthos. Estuarine birds, estuarine food web. Mangroves, sea-grasses, marine fungi. Coastal
Zone Management: Coastal resources i.e. finish, shellfish and non-living resources and their
management. Impact of dredging, mining and pollution on coastal habitats and their
management methods. Remote sensing application in coastal zone management. Coastal zone
regulations.
UNIT-IV
Basics of fish and shellfish health management: Host-pathogen-environment relationship,
Management of culture systems, Environmental stress. Diseases in aquaculture: parasitic,
bacterial, viral and fungal pathogens of fish and shellfish. Water, soil, environmental
parameters and their effects on fish health. Disease in hatcheries and grow out systems.
Epidemiology of diseases, nutritional pathology.
UNIT-V
Defence system in fish and shellfish: Defence systems in fish, innate and acquired immunity,
inflammation response to diseases. Antibody and cell mediated immunity in fish and shellfish.
Techniques in health management: Microbiological, haematological, histopathological,
immunological and molecular techniques. Disease surveillance and reporting.
TEXT BOOKS:
1. Marine Biology. Friedrich H. Sidgwick & Jackson, 1969.
2. Ecology of estuaries. Mc Lusky D.S. Hinmann, 1971.
3. Fish diseases. Aline, W. 1980. Springer – Verlay, Berlin Heidelberg, New York
REFERENCE BOOKS:
1. Fish diseases (Vol. I & II). Schaperclaus, W. 1986. Oxonian Press Pvt. Ltd.
351 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.42
2. Environmental Stress and Fish Diseases. Wedmeyer, G. Meyer, F.P. and Smith, L.,
1999. Narendra Publishing House, Delhi.
3. Fish Pathology. Ronald J. Roberts Ed. 1989. 2nd Edition, Baillere Tindall, London.
4. Principal diseases of Marine Fish and Shelfish. Vol. I & II - Carl, J. Sindermann
1990. 2nd Edition. Academic Press.
352 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.43
SEMESTER – IV
COURSE: M.Sc., Applied Microbiology
SUBJECT TITLE: Practical IV: Environmental and Agricultural microbiology
NUMBER OF HOURS/WEEK: 5Hrs
SUBJECT DESCRIPTION
This Practical paper deals with the Environmental and agricultural aspects of Microbiology
GOAL
This paper gives strong basic knowledge about the microbial load of soil, rhizosphere and
phyllosphere.
OBJECTIVE
On the successful completion of the course, the students will get an overall knowledge in
biofertilizer production and nitrogen fixation.
CONTENTS:
1. Isolation of microbes from rhizosphere
2. Isolation of microorganism from phyllosphere
3. Isolation of phosphate solubilisers from soil
4. Isolation of free living and symbiotic nitrogen fixers
5. Isolation of plant pathogens
(a) Bacteria
(b) Fungi
6. Estimation of BOD
7. Estimation of COD
8. Microbial response to heavy metal contaminants.
9. Water Quality – MPN Technique.
CIA – 40
ESE – 60
353 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.44
10. Production of Bacterial Biofertiliser –Demo
11. Testing of antagonistic activity of soil microbes
REFERENCES:
1. Experiments in Microbiology, Plant pathology & Biotechnology - Aneja, K.R.
(2005) 5th edn. NAI Publ.
2. Microbiology: A lab manual. Cappuccino and Sherman, (2008). 6th edn. Pearson
Education,
3. Microbiology-Practical Lab Manual - Kannan, K. (2002). Palani Paramount
Publishers.
354 Director, SBS
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Page No.45
SEMESTER – IV
COURSE: M.Sc., Applied Microbiology
PROJECT WORK (Duration: 1st December to 28th February)
TOTAL PROJECT HOURS/WEEK: 15
Rules and regulations to be followed:
Project can be carried out at the college premises or at any research institution* or
renowned industry* for a period of three months (*prior permission is required from
the Director).
Allotment of guides will be done as per the Lot system in the third semester.
Review of the project work will be conducted in two stages in the month of Jan and
Feb (after internal and model examinations) for Internal and External students.
For Internal Projects:
The concern guides will maintain attendance. At the end of each month they have to
submit a consolidated attendance to the class in charge.
Requirement of chemicals and glasswares for the project work will be submitted to the
Director with the recommendation from concerned guides on or before 30th of
October. Purchase of expensive items (chemicals or imported chemicals or special
glasswares) will not be permitted.
Students who are doing their project work at CMS shall submit a weekly report of
their research work to their respective guides. Moreover, they have to submit a
monthly report (in MS word format as attachment) to the Director and a copy to be
mailed to cmssbs@gmail.com on or before 30th of each month.
For External Projects:
After getting the permission students can approach the research institute or industry.
Request letter to the Industry or institution can be received from the Director.
CIA – 100 ESE – 50
355 Director, SBS
Anx.PAMB11,2M.Sc.AMB (CMS College) 2017 2016-18 batch
Page No.46
After acceptance, a confirmation/acceptance letter or email to be submitted to the
Director through proper channel (ie., signed by the guide and CC).
Students who are doing their project work in external labs shall submit a weekly report
of their research work to their guide by letter or email. Moreover, they have to submit
a monthly report (in MS word format as attachment) to the Director by email and a
copy to cmssbs@gmail.com on or before 30th of each month.
At the time of completion of their project work in the month of February, students
have to submit original attendance certificate (in letter pad) from research lab or
Industry to the Director duly recommended and forwarded by the guides and CCs.
Based on the recommendations by the guide, attendance will be provided to the
students. The original certificate approved by the Director to be enclosed in the thesis.
Dissertation:
A Common format of the dissertation to be followed by all the students of SBS. A
template of dissertation will be given to each student by email in the month of January.
Final copy will be given for printing after the approval by the concerned guide.
At least one publication of their project work in any journal with ISSN number is
recommended.
Students have to submit three copies of their dissertation duly signed by the guide, CC
and the Director (One original and two copies neatly bound) at the time of viva voce
examination.
Any deviations from above mentioned rules and regulations will not be considered.
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356 Director, SBS
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