buffers used in dna overstretching and unzipping experiments
Post on 14-Jul-2015
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Buffers used in DNA-overstretching and unzipping experiments
• Popping Buffer
• PBS
We primarily use popping buffer and PBS for DNA-unzipping and overstretching experiments
Popping Buffer
Chemicals used in popping buffer:• EDTA:
• Sodium Phosphate• Monobasic:
• Dibasic
• NaCl
• Tween20:
• Water H2O:
• D2O
Quantities and measurements
CalculationsLabView
Weight & Measure
Store
PBS
Chemicals used in PBS: PBS and H2O or D2O
• Dissolve 1 table in 200mL of H2O or D2O
BGBBlotting Grade Blocker
To make 5mg/ml BGB solution in popping buffer:•First wiight out 15mg of BGB poweder•Add 1.5 ml of 1x pop and mix by vortexing•Run through .2μM syringe filter using 3ml syringe.•Now store 5mg/ml BGB in 1x pop buffer (minus amount of protein that stuck to filter) at 3C.This should be good for few weeks.
Filter
Anti-Dig
Microspheres
DNA
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