biotechnology rauful hossain leutrim cahni. recombinant dna gel electrophoresis pcr plasmid
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BiotechnologyRauful Hossain Leutrim Cahni
What is it?
• Biological processes that enable us to quantify molecular biology and can be modified for certain purposes.
It includes• Gel electrophoresis• PCR- Polymerase Chain Reaction• Plasmids• Transformation/Transfection• Recombinant DNA
Recombinant DNA Gel Electrophoresis
PCR Plasmid
Molecules containing DNAfrom two or more organisms
Restriction enzymes added to DNA containing solutionTo cut certain, specific DNA sequences.
Gel is placed with a positive charge at one end, and a negative Charge on the other. Electricity runs through it to separate
DNA by size
DNA primers are addedTo pre-existing strands
To be able and replicate DNA
Circular piece of DNA containingOne chromosome and is easily modified
Via addition of genes
Transformation/Transfection
When a recombinant DNA is inserted into a host cell in order to producemore copies of the desired gene.
Its done by injecting the plasmid throughthe membrane after the bacteria have undergoneheat shock
JEOPARDY
Gel Electrophoresis
Recombinant DNA
Gene Manipulation
PCR Transformation/Transfection
Applications in the world
To cut up certain, specific DNA sequences in an attempt to separate them for evaluation
Restriction Enzymes Collection of DNA fragments
DNA primers added for replication
Recombinant DNA inserted into host cell
Used as innovations to improve lifestyle
To make it easier to observe the DNA through the gel
Same sequences read from opposite sides
Its size DNA or RNA molecule that acts as starting point for 3 prime chain growth
Electroporation, Chemically treating the cell
It can recognize DNA sequences
To separate the DNA strands based on length and size
Molecules that bond and carry foreign DNA to cells
Selectable marker genes
DNA template Opens up the membrane pores to let in the vector
Vaccines contain a viral strand of DNA that has been made non lethal or weakened- through removing genes.
How long certain gene sequences are and whose DNA matches
DNA ligase cDNA RNA transcriptase Normal vectors don’t have genes supporting the expressing of those genes
Health risks, not natural, and obstruction with destiny.
This is when Biologists or forensic specialists analyze a gel electrophoresis experiment.
Plasmids contain no introns, which cause many disruptions to gene addition. But, eukaryotic DNA contains plenty.
Stem cells, can be transformed with recombinant DNA
denaturation, annealing, elongation
Transgenic Animal The gene coding for insulin wouldn’t have been able to be transfected into our somatic cells
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QUESTIONS
Why use restriction enzymes?
What enzymes are used to cut DNA sequences?
What is produced to cut DNA molecules?
What is a genomic library?
What is a vector? What is biotech generally used for?
Why use a dye on the DNA?
What is a palindrome? What makes E.coli easy to manipulate?
What is a primer? What are methods to insert DNA into host cells?
What can electrophoresis do in criminal investigations?
What is the role of the electrical current running through the gel?
What are vectors? What genes confer resistance to antibodies?
What is targeted by the PCR?
What does the heat shock do to the host cell?
How are vaccines made?
What can seeing how DNA separates lead you to conclude?
Which enzyme is used to bind foreign DNA to a vector plasmid
What can be used to make genes transcribed to a particular tissue?
What is not required in PCR reactions?
Why aren’t normal vectors able to be used in transfection?
What ethical issues are anti-biotech?
What are RFLPs? How are plant cells good hosts for recombinant DNA?
What are the 3 main steps in a PCR cycle?
What is an animal that has gained new genetic information from foriegn DNA
Without biotech, why would diabetes be a bigger problem in the world?
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