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Accelerate Protein SamplePreparation and Analysis
www.pall.com/lab
Products for Proteomics, BiopharmaceuticalResearch, Diagnostics, and Protein Chemistry
2
Reliable sample preparation and fractionation processes are
key to the success of proteomic research. Effective steps
can facilitate the discovery of life-saving and life-enhancing
diagnostic and pharmaceutical products. Ineffective steps
can lead to loss of valuable samples and time.
Pall Life Sciences manufactures membranes and
chromatography resins that exhibit high resolution
and binding capacities with low non-specific binding
for the purification and concentration of proteins. We
incorporate these media into devices that use fast, gentle
methods and minimize handling to protect your samples.
Our attention to product quality and performance is
expressed in reproducible, reliable results.
Pall also offers a variety of products for protein detection
including PVDF and nitrocellulose membranes for western
blotting, available as cut membranes or integrated into
multi-well filter plates.
Inspiring Confidence in Critical Proteomic Steps
OptimizedPall offers the largest selection of membrane andchromatography resin chemistries and manufactures these under precise, highly controlled conditions to ensure product quality. Applying a unique blend of LeanManufacturing and Six-Sigma principles, our qualityassurance procedures result in superior products withexceptional lot-to-lot reproducibility. Pall is one of the fewcompanies to control device manufacturing through allstages, from media production to housing material selectionto final device assembly. This gives us a distinct qualityadvantage, allowing us to maximize processing accuracy,speed, safety and reliability.
“The results of my work can change people’s lives.That’s why I count on Pall technologies.”
www.pall.com3
Global reach
Pall Corporation is the largest filtration, separation and
purification company in the world. Our diversity and global
reach provide us with unique application insights and
product development opportunities that we use to benefit
our customers. Pall scientific laboratories worldwide work to
solve complex customer problems, test products, explore
new product applications, and provide ongoing technical
support. Our global manufacturing facilities and distribution
networks ensure product availability, easy ordering, and
fast delivery.
Personalized solutions
With over 50 years of experience, Pall knows how to
combine the optimal materials to improve performance in
a range of applications. Our goal is to provide you with the
best products for your application and help you use them
to their fullest potential. Although we offer thousands of
standard product configurations, Pall product teams
work diligently with our clients and other industry
leaders to develop leading-edge technologies and
optimize product configurations.
Scaleable
Whether you are processing a single sample or detecting
thousands of samples, Pall offers a variety of device
configurations to support your techniques. And when
scale-up is a factor, Pall offers product platforms that
incorporate the same membranes and materials of
construction to allow precise scale-up of processing
volumes from lab to process scale.
Automation compatible
Pall’s high throughput products meet industry guidelines
to ensure compatibility with all standard robotic equipment.
Our filter plates meet the ANSI/SBS X-2004 specifications
for smooth operation and worry-free performance. We
partner with industry-leading equipment manufacturers
to develop products and optimize the automation for
both routine and unique applications.
“When we need support, Pall has theresources that link service to science.”
ARRAYS (Pages 13, 29)
MULTI-WELL FILTER PLATES (Pages 14 - 17)
CENTRIFUGAL DEVICES/SPIN FILTERS (Pages 18 - 19)
PROTEIN PURIFICATION KITS (Pages 20 - 21)
TANGENTIAL FLOW FILTRATION PRODUCTS (Pages 22 - 25)
PREFILTRATION/CLARIFICATION (Pages 26 - 27)
DETECTION PRODUCTS (Pages 28 - 29)
CHROMATOGRAPHY PRODUCTS (Pages 8 - 13)
Ultrogel® AcA, Trisacryl® GF Size Exclusion Chromatography Resins
Ceramic HyperD® Ion Exchange Chromatography Resins
Trisacryl, HyperD, HyperCel™ Affinity Chromatography Resins
SDR HyperD Solvent-Detergent Removal Resins
MEP HyperCel Hydrophobic Charge Induction Chromatography Resins
Mustang® Ion Exchange Membrane Devices
Omega™ Ultrafiltration Membrane for Size Exclusion
AcroPrep™ Plates with Hydrophilic Filtration Membranes
AcroPrep Plates with Ion Exchange Membranes
AcroPrep Plates with Prefilters
AcroPrep Plates with Ultrafiltration Membranes for Size Exclusion
AcroPrep Plates with Glass Fiber
AcroWell™ Plates with Binding Membranes
Microfiltration Centrifugal Devices
Ultrafiltration Centrifugal Devices
Enchant™ Kit for Albumin Depletion
Enchant Kits for IgG Purification
Enchant Multi-Protein Affinity Separation Kit
Minimate™ TFF Capsules
Ultrasette™ Lab TFF Devices
LV Centramate™ Lab TFF Systems
Centramate Lab TFF Systems
Acrodisc® Syringe Filters with Prefilters
VacuCap® Vacuum Filtration Devices
Vivid™ Gene Array Slides
FluoroTrans® PVDF Membranes
BioTrace™ PVDF Membrane
BioTrace NT Nitrocellulose Membrane
UltraBind™ Affinity Membrane
Immunodyne® ABC Membrane
Protein Chip System Based on Arrays
Vivid Gene Array Slides
4
Sample complexity reduction is an important first step to facilitate access to
the low abundant proteins of interest for disease research and diagnostics.
The process for human serum and plasma frequently includes depletion of
highly abundant proteins such as albumin and IgG in combination with other
fractionation technologies prior to 2D-Gel or LC-MS/MS separation. This can
be achieved with a combination of specific affinity ligand-based depletion and
ion exchange chromatography-based fractionation technologies. Pall provides
separation technologies and devices to address these challenging needs in
both low and high throughput applications.
Addressing the Challenges ofProtein Sample Preparation
“Sample preparation...it’s the last thing I want to worry about but the first thing on my mind. That’s why I trust Pall.”
Application guide
Sample Preparation Sample DetectionOptimization
www.pall.com
† In combination with appropriate chromatography resin.
5
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6
Pall has thousands of media chemistries from which to
select, and we are constantly modifying and developing
new chemistries to meet your specific application
challenges. Combined with superior housing materials
in a multitude of configurations and industry-leading
research, development, and manufacturing resources,
Pall is uniquely positioned to deliver products exactly
to your specifications.
Finding the Perfect FitFor Your Application
Versatile separation technologies
Pall gives you more filtration and separation material
options than any other organization in the world. This
means you have the best technologies available to optimize
your application performance. Our quality control procedures
result in the production of media with exceptional lot-to-lot
reproducibility and uniformity to give you the consistent,
accurate results you require.
Reliable device configurations
While the heart of Pall is media development and
manufacturing, the design and manufacture of devices
is the ultimate expression of Pall technologies. This is
where it all comes together – high performance media,
outstanding housing materials, and devices designed to
maximize processing accuracy, speed, safety, reliability,
and ease of use.
The charts on pages 5 and 7 will help you evaluate
media and device options based on your applications
and desired material characteristics.
www.pall.com7
Characteristic
Captu
re,Re
lease
,
Conc
entra
teHig
h Nucle
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idBin
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High Pr
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Low
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Binds
Leuk
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Viral
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Relea
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Hydro
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Compa
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Chem
icals
Chrom
atogra
phy
Filtra
tion
Prefi
ltratio
n
Membrane Name Medium Recommended Applications
Bio-Inert® Membrane Modified Nylon Hydroxyl • • • Filtration of protein solutions, low protein binding,general filtration, clarification of cell lysate and tissue homogenates
Fluorodyne® II Membrane PVDF Hydroxyl • • • • Filtration of low concentration proteins,sample preparation, general filtration
GH Polypro Membrane Polypropylene Proprietary • • • • Time-resolved fluorescence, bead-based assays,(GHP) fluorescent detection of analytes, sample prep
prior to HPLC, general filtration of aqueous and organic solvents
HT Tuffryn® Membrane Polysulfone Proprietary • • • Proven performance in documented procedures
LoProdyne® LP Membrane Nylon Hydroxyl • • • • Filtration of low concentration proteins,sample preparation
Pallflex® Media Glass Fiber Varies • • • • • Prefilters, DNA extraction
PTFE Membrane PTFE PTFE • • • • Vent filters, chemical and molecular synthesis
Supor® Membrane Polyethersulfone Proprietary • • • • General filtration applications, low protein binding,bead-based assays
Supor R Membrane Polyethersulfone RepelTM Treated • • • Vent filters
Versapor® Membrane Acrylic Copolymer Proprietary • • • General filtration applicationson a Nonwoven Support
Versapor R Membrane Acrylic Copolymer Repel Treated • • • Vent filterson a Nonwoven Support
OmegaTM Membrane Modified Proprietary • • • • • Sample preparation, PCR cleanup, sequencing Polyethersulfone cleanup, ultrafiltration separations, nucleic acid and
protein purification, concentration and fractionation
BioSepra® Size Exclusion Varies • • Fractionation, purification, desaltingResins (Ultrogel®, Trisacryl®)
BioSepra Q Ion Exchange Composite Quaternary • •Resins (HyperD®) Material (ceramic) Amine
BioSepra S Ion Exchange Composite Sulfopropyl • •Resins (HyperD) Material (ceramic)
BioSepra DEAE Ion Composite Diethylaminocthyl • •Exchange Resins (HyperD) Material (ceramic)
BioSepra CM Ion Exchange Composite Carboxymethyl • •Resins (HyperD) Material (ceramic)
BioSepra Affinity Resins Varies Varies • • Abundant protein removal, lgG purification,(Blue Trisacryl M, Protein A Ceramic glycoprotein enrichment, lipoprotein purificationHyperD F, Heparin HyperD M,Lysine HyperD, IMAC HyperCel™)
BioSepra Solvent-Detergent Composite Hydrophobic • • Detergent removalRemoval Resins (SDR HyperD) Material (silica) Polymer Moiety
BioSepra Hydrophobic Charge Cellulose Polymer 4-Mercapto- • • Purification of poly and monoclonal antibodies of Induction Chromatography ethyl-pyridine various species, enzymes and recombinant proteinsResins (MEP HyperCel)
Mustang® E Membrane Polyethersulfone Quaternary • • • Removes endotoxin from buffers, water, neutral (positively-charged) Ammonium sugar solutions, and certain biological solutions
Mustang Q Membrane Polyethersulfone Quaternary • • • • • Strong anionic exchanger for DNA clearance,(positively-charged) Amine nucleic acids and negatively-charged proteins,
viral particle purification/concentration
Mustang S Membrane Polyethersulfone Sulfonic Acid • • • • Strong cationic exchanger for positively- (negatively-charged) charged proteins, viral particle
purification/concentration
Biodyne® A Membrane Nylon 6,6 • • • • Macroarrays, microarrays, Southern blots,(amphoteric) northern blots, dot blots, reverse dot blots, DNA
fingerprinting, colony and plaque lifts, ELISA
Biodyne B Membrane Nylon 6,6 Quaternary • • • Macroarrays, microarrays, Southern blots,(positively-charged) Ammonium northern blots, dot blots, reverse dot blots, DNA
fingerprinting, binds negatively-charged molecules
Biodyne C Membrane Nylon 6,6 Carboxyl • • Reverse dot blots, ELISA,(negatively-charged) binds positively-charged molecules
Biodyne Plus Membrane Nylon 6,6 Quaternary • • • Macroarrays, microarrays, Southern blots,(positively-charged) Ammonium northern blots, dot blots, DNA fingerprinting, ELISA
BioTraceTM NT Membrane Nitrocellulose • • • • Western blots, colony/plaque lifts,Southern blots, protein/nucleic acid dot blots,flow-through diagnostic tests, northern blots
BioTrace PVDF Membrane PVDF • • Western blots, protein dot blots
FluoroTrans® PVDF Membrane PVDF • • N-terminal protein sequencing, lowest levels of autofluorescence
FluoroTrans W Membrane PVDF • • Western blots, Southern blots
Immunodyne® ABC Membrane Modified Nylon Proprietary • • • • Oligonucleotide arrays, reverse dot blots,Activated protein arrays, immunoassaysSurface
Leukosorb® Membrane Proprietary Proprietary • • Leukodepletion, nucleic acid extraction,in situ PCR
UltraBindTM Membrane Modified Aldehyde • • Affinity chromatography,Polyethersulfone ELISA, ELISPOT(unsupported)
SurfaceChemistry
Protein purificationand analysis media
Separation Technologies
Microfiltration
Ultrafiltration
Binding
Chromatography
Protein concentration, protein fractionation,contaminant removal, separation based on charge
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portfolio and expands our offering to include resins. The
BioSepra line of chromatography resins greatly simplifies
protein purification and fractionation. These broad lines of
chromatography products exhibit superior performance
and are useful for affinity, ion exchange, size exclusion,
and hydrophobic interaction chromatography (HIC).
Unique mixed-mode BioSepra products also exist to
provide solutions to current sample preparation challenges
such as detergent removal and antibody purification.
Special features
True Scalability
The resins Pall offers for small-scale discovery
applications are the same ones offered to our
customers currently manufacturing biopharma-
ceuticals. The ability to scale up is essential for
those working in drug discovery, development, and
manufacturing. These resins can be used in varying size
chromatography columns, as well as in batch mode for
single prep or high-throughput mode. This is ideal for
quick preps or in situations where optimizing purification
conditions is required.
Versatile Product Line
Pall bottled resins can be used for small and large sample
sizes involving single use or high throughput methods of
purification. Our base resin varies depending on targeted
applications. These resins can be used in combination
with Pall device configurations such as multi-well filter
plates and spin devices.
High Binding Capacities and Fast Flow Rates
By tailoring attributes such as chemistry, pore size,
and resin diameter to specific applications, Pall
chromatography resins exhibit the highest
performance characteristics possible while
ensuring reliable, reproducible protein isolation.
Chromatography Products Expand Separation OptionsPall offers chromatography products to facilitate research
needs, scale-up, and polishing. Our chromatography
solutions are available in resin or membrane formats to
support your specific application. Choose from our extensive
portfolio of media including flat sheet membrane, bulk resin,
and media incorporated into specific product housings. Pall
products allow you to tailor your product selection to the
nature of the purification you desire.
BioSepra® resins facilitate high capacity purification
Chromatography continues to be an essential technology
for the purification of biomolecules. Pall’s recent acquisition
of BioSepra products complements our current technology
www.pall.com9
ApplicationsDetergent removal (SDR HyperD Resin)
Protein fractionation (Q, S, DEAE, CM Ceramic HyperD Resins)
IgG purification from various sample types (Protein A Ceramic HyperD F Resin)
IgG purification from cell culture supernatant (MEP HyperCel™ Resin)
Albumin depletion (Blue Trisacryl® M Resin)
Desalting (Trisacryl GF05 M and Ultrogel® AcA 202 Resins)
Tagged biomolecule purification (IMAC HyperCel Resin)
Reference materialSell Sheet, BioSepra Chromatography Media, PN 33400
BioSepra Ion Exchange Resins Exhibit Extremely High Dynamic Binding Capacity
The HyperD line of ion exchange resins shows high dynamicbinding capacity (50-110 mg BSA/mL of resin for resins testedhere). Dynamic binding capacity is measured in a 1 mL packedcolumn by pumping BSA (anion) or lysozyme (cation) at 5 mg/mLin a suitable binding buffer until the column capacity is exceeded. The capacity is then calculated by estimating the volume of proteinrequired to achieve this “breakthrough” and expressed as mg/mLmedia volume. Anion and cation chemistries are available in both 50 µm (HyperD F resins) and 20 µm (HyperD 20 resins) particlesizes for improved resolution.
Flow Rate (mL/min)Media 1 5 10Q Ceramic HyperD® 20 106.0 mg/mL 91.5 mg/mL 82.5 mg/mL
DEAE Ceramic HyperD F 101.5 mg/mL 87.5 mg/mL 77.5 mg/mL
S Ceramic HyperD F 80.5 mg/mL 61.5 mg/mL 53.5 mg/mL
S Ceramic HyperD 20 98.0 mg/mL 89.5 mg/mL 83.5 mg/mL
CM Ceramic HyperD F 108.0 mg/mL 87.5 mg/mL 73.5 mg/mL
Efficient Removal of Detergents from ProteinSolutions Using the BioSepra SDR HyperD Resin
Detergent Protein SolutionsTriton (DBC = 60-80 mg/mL) IgG AT-III Bovine SerumInitial Conc. (ppm) 10,000 10,000 10,000
Final Conc. (ppm) < 10 < 10 340
Removal Efficiency > 99.9% > 99.9% > 95.2%
SDR HyperD resin binds detergents used in viral inactivation processes (e.g., TnBP and Triton* X-100), as well as other common detergents used in protein procedures (e.g., CHAPS,SDS, ASB14). High recovery of proteins (exclusion limit 10 kDa) is obtained. This product exhibits high adsorption capacity for small hydrophobic molecules and is stable in acidic, polar organic and oxidizing solutions.
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Membranes are recommended in chromatography
applications when there is a need to purify large molecules
or in situations where faster flow is required. Membrane
chromatography is extremely economical because flow rates
are significantly faster than traditional resin chromatography,
decreasing processing time and increasing throughput. Pall’s
Mustang® membranes possess large convective pores and
have dynamic binding capacities that are relatively insensitive
to the effects of high flow rates, even for large molecules
such as plasmids and viruses.
Membrane devices for ionexchange chromatographyspeed processing
Special features
Scaleable
For laboratory-scale applications, Mustang membranes are
available in Acrodisc® units for single samples and AcroPrep™
96-well filter plates for high throughput sample processing.
Devices with Mustang S and Mustang Q membranes can be
scaled up to larger-capacity capsules and cartridges from Pall.
Application-specific Membrane Chemistries
Mustang Q membrane is a strong anion exchanger that
effectively binds plasmid DNA, negatively-charged proteins,
and viral particles. Mustang S membrane is a strong cation
exchanger that effectively binds positively-charged proteins
and viral particles.
Fast Flow Rates for Rapid Separations
Mustang membranes withstand high flow rates to render
faster purification without affecting recovery rates.
www.pall.com11
ApplicationsContaminant removal such as DNA viral particles, host cell proteins, or endotoxin
Isolation via capture and release of plasmid DNA, virus, or target protein from a complex mixture
Protein fractionation or capture
Antibody purification
Reference materialProduct Data, Acrodisc Unit with Mustang S Membrane, PN 33256
Product Data, Acrodisc Unit with Mustang Q Membrane, PN 33255
0
5
10
15
20
25
30
min 0.6 1.2 1.9 2.5 3.1 3.8 4.4 5.0 5.7 6.3Time (min)
Abso
rban
ce28
0nm
(mAU
)
0
5
10
15
20
25
Cond
uctiv
ity(m
s/cm
)
Goat IgG
BSA
AbsorbanceConductivity
Unbound Proteinfrom Goat IgG
0
200
400
600
800
1000
1200
1400
1600
1800
0 5 10 15 20 25
Time (min)
Prot
ein
(mg/
mL)
Elution Peak
54 mg/mL at 0Breakthrough
Acrodisc Unit with Mustang Q Membrane:Resolution with BSA and Goat lgG
Acrodisc Unit with Mustang Q Membrane:Dynamic Binding with BSA
The conditions used to generate data for the resolution graphabove include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 columnvolume (CV); flow rate: 2.3 mL/min (13 cv/min); sampleloading: 4% of total binding capacity.
A solution of 0.524 mg/mL BSA was pumped through theAcrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1minutes and was calculated as 54 mg/mL using:
flow rate (2.3 mL/min) X initial protein BSA concentration (0.524 mg/mL) X time (8.1 min)
membrane bed volume of Mustang Q membrane in 25 mm Acrodisc unit (0.18 mL)
Particle SizeChromatography Type Product Description (Average) Capacity Primary Applications
Size Exclusion (Gel Filtration)
Separation by Molecule Size Bulk Resin
Ultrogel® AcA Ultrogel AcA are polymeric resins for size exclusion composed 100 µm N/A Fractionation, purification of biomolecules of polyacrylamide and agarose, characterized by narrow particle by size, molecular weight determinationsize distribution.
Trisacryl® GF05 M Trisacryl GF are highly hydrophilic copolymer resins designed 60 µm N/A Lowest exclusion limit for desalting and other for medium pressure gel filtration. small molecule removal
Trisacryl GF2000 LS Trisacryl GF are highly hydrophilic copolymer resins designed 120 µm N/A Purification of macromoleculesfor medium pressure gel filtration.
Ion Exchange
Separation by Charge Bulk Resin
Q Ceramic HyperD® 20 Strong anion exchanger. Ceramic HyperD ion exchangers employ 20 µm > 85 mg/mL (4) Polypeptide and plasmid purificationa high capacity hydrogel polymerized within the large pores of a rigid ceramic bead.
S Ceramic HyperD 20 Strong cation exchanger. Ceramic HyperD ion exchangers employ 20 µm > 85 mg/mL (5) Polypeptide purification a high capacity hydrogel polymerized within the large pores of a rigid ceramic bead.
Q Ceramic HyperD F Strong anion exchanger. Ceramic HyperD ion exchangers employ 50 µm > 85 mg/mL (4) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of plasmid, vaccine purification, capture stepa rigid ceramic bead.
S Ceramic HyperD F Strong cation exchanger. Ceramic HyperD ion exchangers employ 50 µm > 75 mg/mL (5) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of vaccine purification, capture stepa rigid ceramic bead.
DEAE Ceramic Weak anion exchanger. Ceramic HyperD ion exchangers employ 50 µm > 85 mg/mL (4) Recombinant proteins, monoclonal antibodies,HyperD F a high capacity hydrogel polymerized within the large pores of plasmid, vaccine purification, capture step
a rigid ceramic bead.
CM Ceramic HyperD F Weak cation exchanger. Ceramic HyperD ion exchangers employ 50 µm > 60 mg/mL (6) Recombinant proteins, monoclonal antibodies,a high capacity hydrogel polymerized within the large pores of vaccine purification, capture stepa rigid ceramic bead.
Q HyperZ® Q HyperZ are specifically designed for high productivity 75 µm > 80 mg/mL (9) Expanded bed and packed bed separationsexpanded bed chromatography and efficient capture of biomolecules directly from crude, unclarified samples in a single pass operation.
CM HyperZ CM HyperZ are specifically designed for high productivity 75 µm ~ 50 mg/mL (10) Expanded bed and packed bed separationsexpanded bed chromatography and efficient capture of biomolecules directly from crude, unclarified samples in a single pass operation.
Membrane Filter Plates and Devices
Mustang® Q Strong anion exchanger. Also available in AcroPrep™ 96 filter plates N/A 50 - 60 mg/mL Protein fractionation350 µL or 1 mL, and Acrodisc® syringe filters.
Mustang S Strong cation exchanger. Also available in AcroPrep 96 filter plates N/A 45 - 50 mg/mL Protein fractionation350 µL or 1 mL, and Acrodisc syringe filters.
Affinity
Separation Using Bulk ResinSpecific Ligands
Blue Trisacryl M Blue Trisacryl M is an affinity chromatographic resin used for the 60 µm HSA: 10 - 15 mg/mL; Albumin depletionpurification of a wide variety of enzymes and proteins such as kinases, BSA: 5 - 7 mg/mL (1)albumin, interferons and some coagulation factors. The basic matrix is Trisacryl GF2000, a macroporous non-ionic resin on which Cibacron* blue is covalently immobilized.
IMAC HyperCel™ IMAC HyperCel uses tridentate IDA (imino-diacetic-acid) as a 90 µm 30 - 60 µmol Tagged biomolecule purificationchelating agent. The ligand is immobilized on the HyperCel Cu++/mL resinbase sorbent, a stable and robust resin.
Protein A Ceramic Protein A Ceramic HyperD F is a high capacity affinity resin 50 µm > 30 mg/mL (2) IgG purification/depletionHyperD F prepared using a rigid proprietary ceramic bead. Recombinant
Protein A is immobilized to a specially formulated hydrogel within the porous ceramic bead.
Heparin HyperD M Heparin HyperD M composite chromatography resin is used to purify 80 µm > 25 mg/mL (3) Purification of coagulation factors,biological molecules that bind to heparin such as coagulation factors, lipoproteins, growth hormones, growth growth factors and lipoproteins. Heparin HyperD M is composed of a factors, nucleic acid binding enzymesporous rigid mineral bead containing heparin bound hydrogel filled pores.
Lysine HyperD Lysine HyperD is used to purify biological molecules that bind to lysine 70 µm N/A Purification of glycoproteinssuch as glycoproteins. Lysine HyperD is comprised of a porous rigid mineral bead containing lysine (L-lysine) bound hydrogel filled pores.
Kits
Enchant™ Albumin For the depletion of albumin from plasma or serum. Includes all N/A > 2 mg albumin Albumin depletion or isolationDepletion Kit buffer and devices needed for 25 purifications. per purification
Enchant Protein A Kit For the purification of IgG. Includes all buffers and devices needed N/A 11 - 19 mg human IgG depletion or purificationfor IgG Purification for 50 purifications. IgG/mL of gel, 6 - 8 mg
mouse IgG/mL of gel
Enchant Protein G Kit For the purification of IgG. Includes all buffers and devices N/A 10 - 15 mg human IgG depletion or purificationfor IgG Purification needed for 10 purifications. IgG/mL of gel
Enchant Multi-Protein For the removal of 99% of albumin and IgG from N/A > 99% removal Albumin and IgG fractionationAffinity Separation Kit serum/plasma samples.
12
Chromatography products selection guide
Particle SizeChromatography Type Product Description (Average) Capacity Primary Applications
Mixed Mode and Hydrophobic Charge Induction (HCIC)
Bulk Resin
MEP HyperCel MEP HyperCel (4-mercapto-ethyl-pyridine) resin is specifically 90 µm > 20 mg/mL (7) Purification/depletion of polyclonal anddesigned for the capture and purification of monoclonal and polyclonal monoclonal antibodies of most speciesantibodies. In contrast to Protein A resins, IgG binding on MEP HyperCel is essentially independent of subclass or species.Weakly binding variants (e.g., murine IgG, rat IgG) are well retained.
SDR HyperD SDR HyperD is a mixed mode of size exclusion, normal phase 80 µm 60 - 80 mg/mL (11) Solvent and detergent removaland reversed phase. It is a unique resin designed to eliminate solvent and detergent while recovering NATIVE protein. SDR HyperD is a composite resin that combines a silica bead moiety filled with long chain aliphatic polymers that are cross-linked to provide a 3D mesh with a low size exclusion limit of 10 kDa which excludes proteins.
Hydroxyapatite
Bulk Resin
HA Ultrogel HA Ultrogel hydroxyapatite resin is composed of cross-linked 120 µm Cytochrome C: Immunoglobulin separation,agarose beads with micro-crystals of hydroxyapatite entrapped > 7 mg/mL (8) glycoproteins, vaccinesin the agarose mesh.
www.pall.com13
Process proteomics centers enhance service
The identification and optimization of protein purification
parameters can be a tedious task. Process proteomics
methodologies can be used to streamline the initial scouting
of protein purification conditions as well as for some of
the optimization steps. Process proteomics is performed
using common chromatographic chemistries (e.g., anion
exchange, cation exchange, IMAC, etc.) on a protein
chip, in the wells of a multi-well filter plate, or using small
columns. Using this approach, multiple binding, washing
and elution conditions can be tested on your sample
simultaneously. Successful scale-up from these small-scale
experiments to traditional column chromatography has
proven to be quite useful.
Pall's Process Proteomics Service Centers assist customers
in selecting and optimizing resins and membranes for the
purification of proteins used in the scale-up and production
of therapeutic proteins and other bioprocess applications.
With access to a large portfolio of both resin and media
technologies, Pall can provide highly integrated solutions
for our customers.
A Unique Combination ofChromatography Modes
(1) capacity determined in PBS buffer using 5 mg/mL
(2) dynamic binding capacity, 10% breakthrough, 100 cm/h, determined using 10 mg/mL hu IgG in PBS, pH 7.4; elution in 0.1 M sodium citrate, pH 2.5; column 4.6 ID x 100 mm
(3) dynamic binding capacity at 600 cm/h, using hu ATIII at 72.5 UI/mL in 20 mM Tris-HCl, 0.3 M NaCl, pH 7.4; elution with 20 mM Tris-HCl, 2 M NaCl, pH 7.4; 10 cm bed height
(4) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
(5) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL lysosome in 50 mM sodium acetate, pH 4.5
(6) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL hu IgG in 50 mM sodium acetate,100 mM NaCl, pH 7.4
(7) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in PBS, flow rate: 60 cm/h
(8) capacity for cytochrome c, determined using 5 mg/mL cytochrome c diluted 50/50 in 1 mM phosphate buffer,pH 6.8; at 12.5 cm/h
(9) dynamic binding capacity, 10% breakthrough determined using 5 mg/mL BSA in 50 mM Tris-HCl buffer,pH 8.6; 150 mM NaCl
(10) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in 50 mM sodium acetate buffer, pH 4.7; 150 mM NaCl
(11) dynamic binding capacity, 10% breakthrough at 300 cm/h, determined using 5 mg/mL Triton* in PBS, pH 7.4
Protein Interaction withCalcium Phosphate
14
As samples get smaller and more numerous, the need for
novel methods to purify proteins and improve assays has
led Pall to develop a broad line of multi-well filter plates
that target specific application challenges. AcroPrep™
and AcroWell™ filter plates feature individually sealed
membranes that eliminate crosstalk and solution weeping.
The proprietary sealing technology allows us to seal virtually
any type of membrane or media configuration into a
device platform to meet ever-changing industry needs.
Special features
Broad Selection to Suit Your Application
The unique nature of protein science results in a variety
of device requirements for each application type. Pall
understands this need and has a full portfolio of 96- and
384-well filter plates that can be optimized for your assay.
Our portfolio includes a selection of single- and multi-layer
membranes, plate colors, well volumes, and outlet tips.
Multi-well Filter Plates Speed Your Rate of Discovery
Pall’s filter plate product line includes two platforms that
address different application needs:
AcroPrep filter plates are engineered with special outlet tipsand splash guards, and can be used for both filtrate- and retentate-based applications. Membranes are individually cut, placed and sealed in the wells using a proprietary sealing process that ensures seal integrity. A distinctive valve technology eliminates sample leaking.
AcroWell filter plates are designed to support retentate and hybridization-based binding applications. These plates are constructed of two membrane layers; the bottom layer protects the upstream functional membrane and acts as a barrier to passive flow.
Using a plate optimized for your application will reduce
sample loss, make automation easy, and add consistency
and reliability throughout your entire process.
www.pall.com15
Automation Compliant
Pall’s plates are designed in accordance with the standards
of the ANSI/SBS X-2004. Rigid construction enables the
plates to be easily maneuvered by robotic instrumentation
and assures that the plates will seat properly on vacuum
manifolds, wash stations, hotel carousels and deck
platforms. Plates are compatible with industry leading
workstations including: Tecan, Qiagen Inc., Tomtec,
PerkinElmer Life and Analytical Sciences, Beckman Coulter
Inc., Caliper Life Sciences, Inc., Waters, Proteodyne, and
Hamilton Company.
Mass Spec Friendly, Chemically Resistant, and Low Binding
The polypropylene housing assembly has been tested to
ensure that the materials of construction do not contribute
to ion suppression/enhancement. In addition, the housing
materials and media have been optimized and tested to
reduce extractables, ensuring that unwanted materials are
not introduced to your sample. The housing is compatible
with a broad range of aqueous and organic liquids, and is
noted for its low biomolecule binding that minimizes non-
specific adsorption of samples to the plates.
No Crosstalk
Specially engineered fluid directors and outlet tips on the
bottom of the AcroPrep plate are designed to reduce the
potential for downstream crosstalk. Elimination of crosstalk
upstream is assured through individually sealing a membrane
in each well using Pall’s proprietary sealing technology. Pall
understands the critical nature of each sample, and we
know that seal failure will cause sample loss. To ensure
integral sealing of each well, we test each lot of product for
seal integrity prior to release. You can be assured that
AcroPrep and AcroWell filter plates will provide a robust
platform that will eliminate concerns of sample loss and
cross contamination.
16
Efficient Optimization of Protein PurificationConditions: AcroPrep™ 96 Filter Plates as “Mini-chromatography” Columns
Although size separation applications can be done effectively
using ultrafiltration, a more specific affinity Immobilized Metal
Affinity Chromatography (IMAC) system is typically needed
to purify tagged biomolecules from crude lysates. Pall
has demonstrated the use of our multi-well filter plates to
perform high throughput IMAC. The low protein binding
and low weeping properties of the AcroPrep 96 filter plate
with low protein binding membrane are ideal for convenient
incubation of the sample directly in wells. The biomolecule-
friendly AcroPrep 96 filter plate allows the researcher to
rapidly and reliably screen numerous samples under a
variety of conditions to determine protein purification
parameters. A single filter plate can be matrixed to:
Screen for metal ions for both custom and pre-charged resins.
Optimize elution conditions.
Optimize resin-to-load ratio.
Aliquots of Ni-NTA resin (Qiagen) were mixed with E. coli inclusionbody lysate containing a His-tagged TEV protease construct(load). The slurry was either incubated in a microfuge tube andthen transferred to a filter plate (left panel) or incubated directly in a well of an AcroPrep 96 filter plate with 0.2 µm Bio-Inert®
membrane (right panel). After washing, the samples were seriallyeluted with either 3 X 200 µL (left panel) or 3 X 50 µL (right panel)of elution buffer. The load (L), flow through (FT), wash (W1 andW2), and elution (E1, E2, and E3) were analyzed by SDS-PAGE.The incubation of sample/resin slurry directly in the wells of thefilter plate gave similar recoveries to those incubated in microfugetubes, allowing the simplification of sample handling. Based onthis observation, the on-plate incubation procedure was used for the subsequent experiments.
The AcroPrep 96 filter plate shows consistent well-to-well
performance, giving protein biochemists an edge in the
development of protein purification protocols.
M L FT W1 W2 E1 E2 E3
Incubation in Centrifuge Tubes
Incubation in AcroPrep 96 Plates
M FT E1 E2 E3
www.pall.com17
Efficient Desalting and High Protein Recovery Using AcroPrep 96 Filter Plates
The efficiency of AcroPrep 96 ultrafiltration filter plates to
remove salts and other small molecules does not diminish
sample recovery. Pall has demonstrated that when using
an AcroPrep 96 filter plate with 10K Omega™ membrane,
the recovery of ovalbumin proteins (45 kDa) at two different
concentrations (0.1 and 1.0 mg/mL) was greater than 90%.
Salt removal efficiencies were also greater than 95%. Using
BSA (66 kDa) and a 30K Omega membrane, similar protein
recoveries and desalting efficiencies were observed.
300 µL of indicated protein solutions was added to wells of 10K or 30K plates. Each test plate was matched to a receiver plate and the assembly spun at 2,000 x g for 40 min. Following centrifugation,retained proteins were collected by adding 300 µL of buffer to eachassay well, then allowing the plate to stand at room temperature for 5 min. before pipetting up and down 10 times to remove sample to fresh tubes. Protein concentration was determined using UVspectrophotometric analysis (n = 3). Percentage of salt removal was determined using a conductivity meter. A representative experiment is shown.
AcroPrep 96 Filter Plate with 10K Omega Membrane
AcroPrep 96 Filter Plate with 30K Omega Membrane
99.1 9497.3 93
0
1020
30
40
5060
70
8090
100
% Salt Removed % Protein Recovered
Ovalbumin (45kDa) 0.1 mg/mL,500 mM NaCl
Ovalbumin (45kDa)1.0 mg/mL200 mM NaCl
99.2 9498.1 95
0
10
20
30
40
50
60
70
80
90
100
% Salt Removed % Protein Recovered
BSA (66kDa) 0.1 mg/mL,500 mM NaCl
BSA (66kDa) 1.0 mg/mL,200 mM NaCl
ApplicationsBead-based applications Lysate clarification
Protein concentration Protein fractionation
Protein purification Gross fractionation
Protein desalting Size exclusion separations
Reference materialSell Sheet, AcroPrep and AcroWell Multi-well, Membrane-bottom Plates, PN 33287
Product Data, AcroPrep Membrane-bottom Plates, PN 33296
Product Data, AcroWell 96 Membrane-bottom Plates, PN 33306
Protocol, Desalting/Buffer Exchange for Biomolecules Using AcroPrep 96 Ultrafiltration Filter Plates, PN 33309
Protocol, Lysate Clearance for Prokaryotic DNA Isolation Using the AcroPrep 96 Filter Plate, PN 33308
Protocol, Automated Purification of Combinatorial Libraries Using AcroPrep 96 Filter Plate with GHP Membrane, PN 33245
Protocol, Biomolecule Binding and Blocking Procedures for AcroWell 96 Filter Plates with BioTrace™ NT and BioTrace PVDF Membranes, PN 33189
Protocol, Using the AcroWell 96 Filter Plate for Receptor/Ligand Binding, PN 33179
Technical Report, IMAC Purification of Polyhistidine-tagged Protein Using the AcroPrep 96 Filter Plate, PN 33354
Technical Report, Automated Plate ELISA and Dot-Blot Assays Using AcroWell 96 Filter Plates and a Robotic Workstation with Integrated Plate Reader, PN 33293
Technical Report, Development of a Fluorescent Ligand-Binding Assay Using the AcroWell Filter Plate, PN 33220
Technical Report, The AcroWell 96 Filter Plate: Low Fluore-scence Background Using the DELFIA* System, PN 33137
Technical Report, The AcroWell Filter Plate Minimizes Crosstalk, see www.pall.com/proteomics
18
Pall’s ultrafiltration centrifugal devices simplify many common
protein handling procedures. These devices provide efficient
concentration and salt removal of samples from 50 µL to
60 mL in just minutes. Choose from membranes that have
been developed to assure low nonspecific biomolecule
binding and provide consistent, high recovery of target
molecules. Ultrafiltration reduces the amount of handling
that can cause damage to samples, leaving concentrated
samples ready for direct incorporation into downstream
applications at critical stages in the discovery process.
Centrifugal Devices Facilitate Pure,Concentrated Product with High Recoveries
Pall’s microfiltration centrifugal devices are used in protein
separation and small-scale general filtration procedures.
These devices can be used in combination with
chromatography resins to create a fast, efficient
method for purifying proteins of interest.
Special features
Rapid Processing
Achieve high recoveries in as little as five to ten minutes.
High Performance Membranes
Omega™ polyethersulfone ultrafiltration membrane provides
higher flow rates and is lower protein binding than competitive
membranes. This results in lower processing time and the
highest possible recoveries.
Low Protein Binding
Devices are constructed of low-binding materials
to maximize sample recovery.
Variety of MWCO’s and Pore Sizes
Available with ultrafiltration membranes for rapid concentrating
and/or desalting of proteins. Also available with low-binding
microfiltration membranes for particulate removal or
chromatography separations.
Easy to Use
Once you have identified the right MWCO or pore size ranging
from 1 kD to 0.45 µm, the devices are color-coded for easy
visual identification.
Ideal for Fast Batch Mode Chromatography Applications
Nanosep® centrifugal devices with microfiltration membrane
serve as a perfect housing for chromatography resin. The spin
device can be filled with the resin of choice to perform the
desired protein purification application.
www.pall.com19
Match device size to sample volume
Pall’s centrifugal devices are available in a range of
sizes to accommodate your specific sample volumes.
AcroPrep™ filter plates can be used with smaller sample
volumes in similar applications.
Device Sample VolumeAcroPrep 384 filter plate < 100 µL
AcroPrep 96 filter plate, 350 µL < 350 µL
AcroPrep 96 filter plate, 1 mL < 1 mL
Nanosep device < 0.5 mL
Microsep™ device 0.5 - 3.5 mL
Macrosep® device 3 - 15 mL
Jumbosep™ device 15 - 60 mL
Easy-to-assemble stirred cell systems process 2 to 150 mL
Pall’s Stirred Cell Systems
provide a versatile format that
can be disposed of when working
with biologically hazardous or
radioactive materials, or cleaned
and reused up to 20 times.
These devices are 100% integrity tested to ensure the
membrane and cell reservoir are integral. Ultrasonically
sealed membranes eliminate the need for O-rings that can
leak. The devices are easy to assemble, use and clean up,
and feature as much as 50% more effective filtration area
than conventional stirred cells of the same volume.
ApplicationsConcentrate, purify and desalt peptides and proteins
Separate proteins from acrylamide gels
Prepare samples for HPLC analysis
Fractionate proteins
Reference materialSell Sheet, Centrifugal Devices, PN 33327
Product Data, Centrifugal Devices for Ultrafiltration and Microfiltration, PN 32984
Product Data, Stirred Cell Systems and Ultrafiltration Membrane Disc Filters, PN 32985
Protocol, Desalting/Buffer Exchange for Biomolecules Using AcroPrep 96 Ultrafiltration Filter Plates, PN 33309
Protocols, Nanosep Centrifugal Devices, PN 32989
Technical Report, Fast and Efficient Elution of Proteins from Polyacrylamide Gels Using Nanosep Centrifugal Devices, see www.pall.com/proteomics
Technical Report, Purification and Handling of DNA Fragments, see www.pall.com/proteomics
Technical Report, Nanosep Centrifugal Ultrafiltration Devices and PCR: Before and After, see www.pall.com/proteomics
Technical Report, Single-tube DNA Purification and Cloning Using Ultrafiltration Devices, see www.pall.com/proteomics
Nanosep Devices Exhibit Fast Spin Times and High Recoveries
Samples of 0.5 mL of a 1.0 mg/mL solution were centrifuged at 14,000 x g andconcentrated to a volume of 10 to60 µL using Nanosep centrifugaldevices with Omega membrane.
MWCO 3K 10K 30K 100K 300KSolute Solute MW (Kd) Spin Time (min.) 15 10 8 5 3Vitamin B12 1,335 % Recovery 7 - - - -
Aprotinin 6,200 % Recovery 99 51 11 - -
Cytochrome C 12,400 % Recovery 100 89 77 1.8 -
Chymotrypsinogen A 25,000 % Recovery - 97 94 2.1 -
Ovalbumin 45,000 % Recovery - 97 92 3 -
BSA 67,000 % Recovery - - 100 26 1.5
Phosphorylase B 97,400 % Recovery - - 95 91 1
IgG 156,000 % Recovery - - - 97 1.5
Thyroglobulin (1 mg/mL) 677,000 % Recovery - - - 100 91
20
The first step in isolating new drug targets is critical, and
reliable purification with minimal loss is key. Biospecific
affinity ligand technologies such as Pall’s Enchant Protein A
or G antibody purification/depletion kits are widely used in
immunoglobulin purification for research and therapeutic
applications. Enchant Protein Purification Kits rapidly deplete
unwanted abundant proteins and unmask low abundant
biomarkers from human and animal-derived serum and
plasma samples. Alternately, these same kits can be used
to collect the abundant protein fraction for further analysis.
Convenient kit formats eliminate the need to handle messy
slurries or deal with column packing. These all-in-one kits
include the protocol, purification columns and buffers, and
offer one of the lowest costs per sample available.
Enchant™ Protein Purification Kits Expedite Proteomic Sample Prep
Special features
Enchant Albumin Depletion or Purification KitEffectively process up to 100 µL of serum or plasma in 5 simple steps.
Remove > 2 mg of albumin per column.
Remove albumin from multiple species including human, rat, goat, calf, and bovine.
Albumin can be discarded or recovered for further analysis.
Enchant IgG Depletion or Purification Kits High binding. Bind between 11-19 mg of human IgG/mL of gel (typical binding capacity).
Purify a variety of IgG molecules from a broad range of species including human, horse, mouse, rat, cow, goat, etc.
Each column can be regenerated and used for ten purifications.
Convenient kits contain all components necessary to affinity purify or deplete IgG with either Protein A or Protein G affinity resin from ascites fluid, serum or plasma.
IgG can be discarded or recovered for further analysis.
Enchant Multi-Protein Affinity Separation KitAchieve 99% removal of albumin and IgG from human serum or plasma samples.
High specificity. Will not remove low abundant, low molecular weight biomarkers.
No loss of protein on the fractionation columns.
Can process up to 50 µL human serum or plasma in just 15 minutes.
Albumin and IgG fractions can be further fractionated and analyzed.
Reference materialSell Sheet: Enchant Albumin Depletion Kit, PN 33355
Sell Sheet: Enchant IgG Purification Kits, PN 33356
Product Data, Enchant Life Science Kits Albumin Depletion, see www.pall.com/proteomics
Product Data, Enchant Life Science Kits IgG Purification, see www.pall.com/proteomics
www.pall.com21
Effective Depletion of Human Serum Albumin (HSA)and IgG from Plasma and Serum Samples
Depletion of both IgG and HSA using Enchant Abundant ProteinDepletion kits. One mL of human plasma was processed using theEnchant Protein A IgG purification kit. Then, 30 µL of the columnflow through was treated with the Enchant albumin depletion kit.Samples were loaded onto 4-12% SDS-PAGE gels and resolved in MOPS/SDS running buffer under non-reducing conditions.Proteins were visualized with colloidal Coomassie* blue. Left panelshows results for human plasma; right panel shows results forhuman serum. Lane 1 in both panels shows starting material, 1 µL. Lane 2 shows IgG removal from the Protein A column flowthrough. Lane 3 shows subsequent removal of albumin.
1 2 3 1 2 3
IgG
HSA
Visualize Low Abundant Proteins with EffectiveAlbumin Depletion
Two dimensional gel electrophoresis (2DGE) analysis of humanplasma following treatment using the Enchant Albumin Depletion kit.Briefly, 20 µL of human plasma was diluted with 30 µL of bindingbuffer and added to the Enchant albumin depletion column.Sample was incubated for 10 minutes at room temperature thenspun at 12,000 x g for one minute. Filtrate was recovered, addedback to resin, incubated for 2 minutes and spun again. Therecovered filtrate and starting material were analyzed by 2DGE byfocusing in the first dimension on a pH 4-7 IPG strip then resolvingthe second dimension on an 8-16% tris-glycine gel under reducingconditions. Proteins were visualized with colloidal Coomassie blue.
Versatile Purification and Depletion of IgG and HSA from Multiple Species: Protein A Kit
Purification of different species’ IgG’s using Enchant Protein A IgGPurification kit. One mL of plasma (human, rat) or serum (rabbit,mouse) was processed according to insert instructions. The plasma or serum starting material (1 µL – Lanes 1, 3, 5, 7) and one eluatefrom each species with an A280 of approximately 1.0 (10 µL – Lanes2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolvedunder non-reducing conditions in MOPS/SDS running buffer. Proteinswere visualized with colloidal Coomassie blue staining. Efficientdepletion occurred with each species processed.
1 2 3 4 5 6 7 8
IgG
IgG HC
IgG LC
Human Rabbit Mouse Rat
Versatile Purification and Depletion of IgG and HSA from Multiple Species: Protein G Kit
Purification of different species’ IgG’s using Enchant Protein G IgGPurification kit. One mL of plasma (human, rat) or serum (rabbit,mouse) was processed according to insert instructions. The plasmaor serum starting material (1 µL – Lanes 1, 3, 5, 7) and one eluatefrom each species with an A280 of approximately 1.0 (10 µL – Lanes2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolvedunder non-reducing conditions in MOPS/SDS running buffer. Proteinswere visualized with colloidal Coomassie blue staining. Efficientdepletion occurred with each species processed.
IgG
IgG HC
IgG LC
Non-depleted
Depleted
Plasma Serum
1 2 3 4 5 6 7 8
Human Rabbit Mouse Rat
22
Tangential Flow Filtration (TFF) is a rapid and efficient
method for separating and purifying biomolecules. Pall’s
TFF products combine low protein binding ultrafiltration or
microfiltration membranes with optimized flow path design
to quickly concentrate samples while achieving high
concentration factors for sample volumes from 10 mL
to thousands of liters. Systems are designed for easy set
up and use. By incorporating the same path length and
materials of construction throughout our TFF product line,
conditions established during pilot-scale trials can easily be
applied to process-scale applications.
Increase Productivity Using Tangential Flow Filtration
Special features
Easy Set Up and Use
Simply connect the TFF device to a pump and pressure
gauge(s), add sample, and process.
High Concentration Factors
Low hold-up volumes allow high concentration factors
to be achieved from small starting volumes.
Fast and Efficient Processing
Higher concentrations can be achieved in less time than with
centrifugal devices or stirred cells. Sample concentration and
diafiltration can be achieved on the same system, saving
time and avoiding product loss.
Scale Up or Down
Identical fluid path lengths and materials of
construction allow precise linear scale-up to
larger systems. The membrane area of a
smaller device can be increased simply by
connecting multiple devices or adding cassettes.
Assuring predictable performance saves time when
scaling a process from pilot to production.
Economical
TFF devices and cassettes can be cleaned and reused, or
disposed of after a single use. A simple integrity test can be
performed to confirm that membrane and seals are intact.
www.pall.com23
General Product Selection Based on Starting Sample Volume
Recommended Typical Filtrate Retentate Flow Rate/ Minimum
TFF Capsule Membrane Area/ Flow Rate** at Capsule or Cassette Starting Sample Concentratedor Cassette* Capsule or Cassette 50 LMH 20 °C for Screen Channel Volume Range Volume***
LAB SCALE/SCALE-UPMinimate™ 50 cm2 (0.05 ft2) 4 mL/min 30 - 80 mL/min 25 - 1000 mL < 10 mL
LV Centramate™ 0.01 m2 (0.1 ft2) 8 mL/min 60 - 80 mL/min 40 - 2000 mL 10 mL
LV Centramate 0.02 m2 (0.2 ft2) 15 mL/min 120 - 160 mL/min 60 - 4000 mL 15 mL
PROCESS DEVELOPMENT AND SMALL-SCALE PRODUCTIONUltrasette™ 0.084 m2 (0.9 ft2) 4 L/hr 1200 - 1500 mL/min 0.2 - 5 L 100 mL
Centramate 0.093 m2 (1.0 ft2) 4.6 L/hr 600 - 800 mL/min 0.2 - 25 L 100 mL
* Data is per unit or cassette. Centramate holder can hold fivecassettes. Other column data can be calculated by multiplying table values by the number of cassettes installed in the holder.
** Typical filtrate flow rate is based on an average filtrate flow rate of 50 LMH and a process time of about four hours. Actual valuemay be higher or lower depending on the MWCO of membrane,
sample composition and viscosity, operating conditions, i.e.,transmembrane pressure, cross flow rate, temperature, etc.
*** Minimum concentrated volume depends on system hold-upvolume, reservoir design and pump type and speed. Smallervolumes can be achieved by minimizing tubing lengths and use of properly sized components, tubing, fittings, etc.
Optimize performance byselecting the proper device
Choosing the appropriate cassette or device size depends
on the total sample volume, the required process time, and
the desired final sample volume. Performance parameters
for Pall’s laboratory TFF devices are presented below.
24
Minimate™ TFF system streamlines lab-scale concentration, desalting, andbuffer exchange processes
The Minimate TFF system efficiently concentrates samples
from up to one liter to as little as 5 mL, enabling high
concentration factors. Subsequent desalting or buffer
exchange steps can be run on the same system with
minimal user intervention. The system works with Pall’s
Minimate TFF capsule, a disposable device designed to
accelerate and simplify scale-up applications.
www.pall.com25
Gain Precise Control of Protein Concentration with the Minimate TFF System and a LiquidChromatography System
-50
0
50
100
150
200
250
5 10 15 20 25 30 35 40
Time (min)
Abso
rban
ce(m
AU)
0
5
10
15
20
25
Cond
uctiv
ity(m
S/cm
)
Conductivity
A280
A600
The concentration of a 1 mg/mL BSA solution was accomplishedby leaving the diafiltration feed line open to air. The run, using aMinimate 10K capsule, was processed at 25 mL/min recirculationrate. Salt, protein (A280) and turbidity (A600) were monitored using in-line sensors.
Facilitate Sequential Buffer Exchange andConcentration with the Minimate Capsule and a Liquid Chromatography System
-100
100
300
500
700
900
1100
80 100 120 140 160 180 200 220 240 260
Time (min)
Abso
rban
ce(m
AU)
0
10
20
30
40
50
60
70
80
90
100
Cond
uctiv
ity(m
S/cm
)
Conductivity
A280
A600
Sequential diafiltration followed by concentration was documentedfor a single run using a 1 mg/mL BSA solution in 1X PBS, 1MNaCl starting solution. The run using a Minimate 10K capsule wasprocessed at 25 mL/min recirculation rate with the buffer exchangefrom high salt to low salt. The diafiltration buffer feed line was thenopened to air, allowing the concentration of the sample. Salt,protein (A280) and turbidity (A600) were monitored using in-line sensors.
ApplicationsConcentration and desalting proteins and peptides
Protein fractionation
Sample preparation prior to or post chromatography
Reference materialBrochure, Improve Biopurification Processes, PN 33377-BIO
Data Sheet, Minimate Tangential Flow Filtration System and Minimate TFF Capsule, PN 33366
Technical Report, Increased Productivity Using Minimate Capsules to Replace Stirred Cell Systems, PN 33342
Technical Report, The Partnership of the Minimate TFF Capsulewith Liquid Chromatography Systems Facilitates Lab-scale Purifications and Process Development Through In-line Monitoring, PN 33339
Technical Report, Desalting and Buffer Exchange by Dialysis, Gel Filtration or Diafiltration, PN 33290
Technical Report, Diafiltration: A Fast, Efficient Method for Desalting or Buffer Exchange of Biological Samples, PN 33289
Technical Report, Introduction to Tangential Flow Filtration for Laboratory and Process Development Applications, PN 33213
Minimate TFF Capsule Frequently Asked Questions, see www.pall.com/proteomics
26
Although a basic filtration concept, the clarification and
prefiltration of samples remains an important function within
proteomics. When filtration is used as a prefilter, matching
the proper filter media and device to the application is
critical. Here, larger pore size filter materials are used to filter
solutions prior to more detailed analysis. When selecting the
best product for your application, numerous factors need to
be considered. Sample viscosity, sample volume and
sample recovery are just some of the aspects that will drive
the selection of the optimal device.
Simplify Prefiltration and Clarification Procedures
Device
VacuCap® PFBottle-top Filters
Serum Acrodisc®Syringe Filters
Acrodisc PSFSyringe Filters
Acrodisc PFSyringe Filters
AcroPrepTM 96Filter Plates
46284638
4524 4525
AP-4523
46584187
5053 5041 5046
Sample Viscosity
Recommended Part Numbers
Sam
ple
Volu
me
Pall offers a number of media and device options for fast,
effective filtration with minimal sample hold-up for both
single sample and high throughput processing. From
sample volumes of a few microliters to multiple liters, Pall
can supply the best product solution for your application.
www.pall.com27
Rapid, Effective Clarification of Plasmid Purification Lysates Increases Recoveries
The use of filtration for the clarification of plasmid purification
lysates enables a rapid and effective alternative to centrifugal
sedimentation. The AcroPrep 96 filter plate with an integral
prefilter configuration improves the time and effectiveness
of filtration by allowing the prefiltration of viscous samples.
The use of an integral prefilter shortens filtration times,
allows greater flow rates, and results in high recoveries
equal to sedimentation.
Filtration times were measured for 100 µL and 200 µL lysatesamples. Average flow rates were calculated for the AcroPrep 96filter plate with Bio-Inert® membrane (PN 5042), AcroPrep 96 filterplate with Glass Fiber prefilter over Bio-Inert membrane (PN 5046),and a Competitor hydrophilic plate containing a floating prefilter(COMP). Error bars indicate standard error (n=8).
For the complete protocol see, “Lysate Clearance for
Prokaryotic DNA Isolation Using the AcroPrep 96 Filter
Plate,” PN 33308.
12
10
8
6
4
2
0
Flow
Rate
(mL/
sec)
100 µL 200 µLVolume Filtered
PN 5042PN 5046COMP
Built-in Prefilter Enhances Throughput of Viscous,Particulate-laden or Proteinaceous Solutions
The more particulate-laden and/or the higher the protein
concentration in a solution, the more difficult it is to filter.
These types of liquids may clog filters prematurely. The
integration of a prefiltration media dramatically increases both
the throughput and flow rates of proteinaceous solutions.
Acrodisc and Acrodisc PF syringe filters with 0.2 µm Supor®
membrane were challenged with bovine serum or a bacterialculture (107 cfu/mL) at a constant pressure of 1.4 bar (140 kPa, 20 psi). The use of a prefilter (PF) device significantly increased throughput and flow rate.
100%
Calf
Seru
m(m
L)
Time (sec)
0
2
4
6
8
10
0 5 10 15 20
B.di
min
uta
(mL)
Time (sec)
0
10
20
30
40
50
0 5 10 15 20
0.2 µm PF
Reference materialBrochure, Improve Biopurification Processes, PN 33377-BIO
Product Data, Sterile Acrodisc Syringe Filters, PN 33175
Product Data, VacuCap and VacuCap PF Vacuum Filtration Devices, PN 32914
Product Data, AcroPrep Membrane-bottom Filter Plates, PN 33296
Technical Report, Syringe Filter Efficiency and Effect of Filtration on HPLC Column Life, PN 33312
28
For protein characterization, the use of membrane tech-
nology is ideal for obtaining detailed structural information.
Pall offers an impressive range of membranes compatible
with all common protein detection procedures. Our
membranes feature superior binding capacities and
extremely low background to facilitate the transfer and
retention of rare and low-abundant proteins for detection
and further characterization.
Pall’s strict quality manufacturing specifications ensure
consistent, precise membrane performance from
lot to lot and blot to blot. Our membranes set industry
standards for reproducible results, durability, and high
signal-to-noise ratios. Choose from a range of commonly
used formats including rolls, discs, sheets, or custom cuts.
PVDF Membranes
FluoroTrans®, FluoroTrans W, and BioTrace™ PVDF
membranes are hydrophobic in nature and strongly bind
proteins of interest. FluoroTrans membrane is recommended
for protein sequencing and is compatible with all reagents
involved in the Edman reaction. FluoroTrans W and BioTrace
PVDF membranes exhibit low background and high tensile
strength, and are the best membranes available for detection
of protein after Western transfer. All FluoroTrans membranes
exhibit exceptionally low burn through.
Nitrocellulose Membranes
BioTrace NT pure nitrocellulose membrane can be used
for Western transfers and ELISpot assays. The membrane
offers high protein binding and is compatible with a wide
range of protein stains and immunodetection techniques.
Activated Membranes
Pall offers two activated membranes for covalent protein bind-
ing: UltraBind™ modified polyethersulfone has a high ratio of
covalent to non-covalent protein binding; Immunodyne® ABC
membrane offers the sharp spot geometry of nylon with a
proprietary covalent attachment chemistry.
Obtain High Sensitivity for Protein Detection
Gain sensitive, detailed structural information
Rabbit reticulocyte lysate (GE Healthcare) was loaded in lanes of polyacrylamide gels at full strength, 1/3 and 1/10 dilutions. After electrophoresis, proteins were transferred to membranes.Membranes were stained with 0.1% Amido Black, 45% methanol,2% acetic acid for 4 minutes and were then destained for 5 minutes with two changes of 90% methanol, 2% acetic acid. Stained membranes were rinsed in water and air dried.
FluoroTrans Membrane has Excellent Sensitivity,Signal, and Extremely Low Background in Western Transfers
FluoroTransmembrane
FluoroTrans Wmembrane
Competitor PVDF membrane
Avoid membrane damage, make filter handling easy
Pall’s forceps feature flat, smooth tips that gently handle
membrane filters. Polypropylene finger grips provide a
comfortable and secure hold. Choose traditional black
or multi-colored finger grips for forceps that are easy
to identify, track, and see on the lab bench.
www.pall.com29
High Sensitivity Double Antibody Assay for HSR Using Vivid Microarray Slides
1st antibody: Mouse anti-human HSA. 2nd antibody: Goat anti-mouse IgGconjugated to APC. Eight dilutions of HSAwere printed onto a VividGene Array slide using a Genomic Solutions G3 robot; 4x4 blocks; 4 blocks per dilution.Spots are visible at the lowest level printed (10 pg).
pg/spot
1,250
625
312
156
78
39
20
10
Direct fluorescent detection with Vivid™ microarray slides
Vivid Microarray Slides exhibit good signal to noise and
dose response along with excellent sensitivities when used
for double antibody type assays. Easy protocols, simple
immobilization steps, and automation-friendly design make
Vivid slides the ideal choice for consistent, detectable results.
ApplicationsWestern transfers
N-terminal protein sequencing
ELISA
Affinity separation
ELISpot Assays
Reference materialProduct Data, Membranes for Transfer and Immobilization, PN 33082
Product Data, AcroWell 96 Membrane-bottom Filter Plates, PN 33306
Protocol, Double Antibody Nanoimmunoassay with Direct Fluorescent Detection, PN 33273
Protocol, Biomolecule Binding and Blocking Procedures for AcroWell 96 Filter Plates with BioTrace NT and BioTrace PVDF Membranes, PN 33189
Protocol, Transfer and Detection Procedures for Pall Life Sciences Membranes, PN 33167
Technical Report, Automated Plate ELISA and Dot-Blot Assays Using AcroWell 96 Filter Plates and a Robotic Workstation with Integrated Plate Reader, PN 33293
AcroWell™ 96 filter plates expand detection potential
For protein detection in a multi-well format, AcroWell 96 filter
plates are excellent for parallel or automated applications.
Choose plates with BioTrace NT (nitrocellulose) or PVDF
membrane. See pages 14 – 17 for more information on
filter plates.
30
Ordering InformationChromatography resins(lab scale volumes)
Blue Trisacryl® M Affinity Chromatography ResinPart Number Description Packaging
25896-051 Blue Trisacryl M 5 mL
25896-045 Blue Trisacryl M 25 mL
25896-010 Blue Trisacryl M 100 mL
25896-028 Blue Trisacryl M 1000 mL
DEAE and SP Spherodex® LS Silica/DextranComposite Ion Exchange ResinsPart Number Description Packaging
26455-023 DEAE Spherodex LS 100 g
20080-024 SP Spherodex LS 100 mL
HA Ultrogel® HydroxyapatiteChromatography ResinPart Number Description Packaging
24775-075 HA Ultrogel Hydroxyapatite 5 mL
24775-082 HA Ultrogel Hydroxyapatite 25 mL
24775-025 HA Ultrogel Hydroxyapatite 100 mL
24775-041 HA Ultrogel Hydroxyapatite 1000 mL
Heparin HyperD® M Affinity Chromatography ResinPart Number Description Packaging
20029-062 Heparin HyperD M 5 mL
20029-039 Heparin HyperD M 25 mL
20029-021 Heparin HyperD M 100 mL
20029-013 Heparin HyperD M 1000 mL
IMAC HyperCel™ Chromatography ResinPart Number Description Packaging
20093-069 IMAC HyperCel 5 mL
20093-010 IMAC HyperCel 25 mL
20093-028 IMAC HyperCel 100 mL
Lysine HyperD Chromatography ResinPart Number Description Packaging
20059-058 Lysine HyperD 5 mL
20059-036 Lysine HyperD 25 mL
20059-028 Lysine HyperD 100 mL
20059-010 Lysine HyperD 1000 mL
MEP HyperCel Hydrophobic Charge Induction Chromatography (HCIC) ResinPart Number Description Packaging
12035-069 MEP HyperCel 5 mL
12035-010 MEP HyperCel 25 mL
12035-028 MEP HyperCel 100 mL
12035-036 MEP HyperCel 1000 mL
Methyl Ceramic HyperD F Chromatography ResinPart Number Description Packaging
20051-033 Methyl Ceramic HyperD F 25 mL
20051-025 Methyl Ceramic HyperD F 100 mL
20051-071 Methyl Ceramic HyperD F 1000 mL
Protein A Ceramic HyperD F AffinityChromatography ResinPart Number Description Packaging
20078-036 Protein A Ceramic HyperD F 5 mL
20078-028 Protein A Ceramic HyperD F 25 mL
20078-010 Protein A Ceramic HyperD F 100 mL
20078-044 Protein A Ceramic HyperD F 1000 mL
SDR HyperD Solvent-Detergent Removal Chromatography ResinPart Number Description Packaging
20033-065 SDR HyperD 5 mL
20033-031 SDR HyperD 25 mL
20033-023 SDR HyperD 100 mL
20033-015 SDR HyperD 1000 mL
Q and CM HyperZ® Ion Exchange ResinsPart Number Description Packaging
21012-010 Q HyperZ 50 g
21012-020 Q HyperZ 250 g
21012-030 Q HyperZ 1 kg
21011-010 CM HyperZ 50 g
21011-020 CM HyperZ 250 g
21011-030 CM HyperZ 1 kg
Q, S, DEAE, CM Ceramic HyperD,CM Trisacryl M Ion Exchange ResinsPart Number Description Packaging
20040-051 Q Ceramic HyperD 20 5 mL
20040-044 Q Ceramic HyperD 20 25 mL
20040-036 Q Ceramic HyperD 20 100 mL
20040-028 Q Ceramic HyperD 20 500 mL
20040-010 Q Ceramic HyperD 20 1000 mL
20038-055 S Ceramic HyperD 20 5 mL
20038-048 S Ceramic HyperD 20 25 mL
20038-030 S Ceramic HyperD 20 100 mL
20038-022 S Ceramic HyperD 20 500 mL
20038-014 S Ceramic HyperD 20 1000 mL
20066-098 Q Ceramic HyperD F 5 mL
20066-031 Q Ceramic HyperD F 25 mL
20066-023 Q Ceramic HyperD F 100 mL
20066-015 Q Ceramic HyperD F 1000 mL
20062-089 S Ceramic HyperD F 5 mL
20062-030 S Ceramic HyperD F 25 mL
20062-022 S Ceramic HyperD F 100 mL
20062-014 S Ceramic HyperD F 1000 mL
20067-070 DEAE Ceramic HyperD F 5 mL
20067-039 DEAE Ceramic HyperD F 25 mL
20067-021 DEAE Ceramic HyperD F 100 mL
20067-013 DEAE Ceramic HyperD F 1000 mL
20050-084 CM Ceramic HyperD F 5 mL
20050-035 CM Ceramic HyperD F 25 mL
20050-027 CM Ceramic HyperD F 100 mL
20050-019 CM Ceramic HyperD F 1000 mL
26708-016 CM Trisacryl M 300 mL
Trisacryl GF Size Exclusion Chromatography ResinsPart Number Description Packaging
25914-060 Trisacryl GF05 M 100 mL
25914-037 Trisacryl GF05 M 1000 mL
25916-040 Trisacryl GF05 LS 100 mL
25916-016 Trisacryl GF05 LS 1000 mL
26064-055 Trisacryl GF2000 M 100 mL
26064-022 Trisacryl GF2000 M 1000 mL
26065-045 Trisacryl GF 2000 LS 100 mL
26065-011 Trisacryl GF 2000 LS 1000 mL
Ultrogel AcA Size Exclusion Chromatography ResinsPart Number Description Packaging
23013-025 Ultrogel AcA 22 100 mL
23013-014 Ultrogel AcA 22 1000 mL
23015-025 Ultrogel AcA 34 100 mL
23015-019 Ultrogel AcA 34 1000 mL
23022-024 Ultrogel AcA 44 100 mL
23022-015 Ultrogel AcA 44 1000 mL
23019-023 Ultrogel AcA 54 100 mL
23019-011 Ultrogel AcA 54 1000 mL
24892-022 Ultrogel AcA 202 100 mL
24892-010 Ultrogel AcA 202 1000 mL
Ion exchange chromatography devices
AcroPrep™ 96 Filter Plates, 350 µL WellPart Number Description Packaging
5047 Mustang® Q membrane, natural 10/pkg
5048 Mustang S membrane, natural 10/pkg
AcroPrep 96 Filter Plates, 1 mL WellPart Number Description Packaging
5062 Mustang Q membrane, natural 5/pkg
5063 Mustang S membrane, natural 5/pkg
Acrodisc® Units, Mustang MembranesPart Number Description Packaging
MSTG25Q6 Acrodisc unit with Mustang Q 10/pkgmembrane, 0.8 µm, 25 mm,(blister non-sterile packs)
MSTG25S6 Acrodisc unit with Mustang S 10/pkgmembrane, 0.8 µm, 25 mm,(blister non-sterile packs)
www.pall.com31
Multi-well filter plates
AcroPrep™ 96 Filter Plates, 350 µL WellPart Number Description Packaging
5033 3K Omega™ membrane, natural 10/pkg
5034 10K Omega membrane, natural 10/pkg
5035 30K Omega membrane, natural 10/pkg
5036 100K Omega membrane, natural 10/pkg
5045 0.2 µm GHP membrane, natural 10/pkg
5030 0.45 µm GHP membrane, natural 10/pkg
5043 0.45 µm GHP membrane, white 10/pkg
5044 0.45 µm GHP membrane, black 10/pkg
5037 0.2 µm PTFE membrane, natural † 10/pkg
5038 0.45 µm PTFE membrane, 10/pkgnatural †
5042 0.2 µm Bio-Inert® membrane, 10/pkgnatural
5046 3.0 µm glass fiber media/0.2 µm 10/pkgBio-Inert membrane, natural
5031 1.0 µm glass fiber, natural †† 10/pkg
5032 1.0 µm glass fiber, white †† 10/pkg
5029 0.45 µm Supor® membrane, 10/pkgnatural
5039 1.2 µm Supor membrane, natural 10/pkg
5041 Prefilter material/1.2 µm 10/pkgSupor membrane, natural
5047 Mustang Q ion exchange 10/pkgmembrane, natural
5048 Mustang S ion exchange 10/pkgmembrane, natural
5049 54 µm screen, natural 10/pkg
AcroPrep 96 Filter Plates, 1 mL Well Part Number Description Packaging
5052 0.2 µm GHP membrane, natural 5/pkg
5054 0.45 µm GHP membrane, natural 5/pkg
5055 0.2 µm PTFE membrane, 5/pkgnatural ††
5056 0.45 µm PTFE membrane, 5/pkgnatural ††
5051 1.0 µm glass fiber media, 5/pkgnatural †
5053 3.0 µm glass fiber 5/pkgmedia/0.2 µm Bio-Inert membrane, natural
5062 Mustang Q ion exchange 5/pkgmembrane, natural
5063 Mustang S ion exchange 5/pkgmembrane, natural
AcroWell™ 96 Filter Plates, 350 µL WellPart Number Description Packaging
5020 0.45 µm GHP membrane, natural, 10/pkg350 µL well
5021 0.45 µm GHP membrane, white, 10/pkg350 µL well
5022 0.2 µm BioTrace™ NT 10/pkgmembrane, white
5025 0.2 µm BioTrace NT membrane, 10/pkgblack
5023 0.45 µm BioTrace PVDF 10/pkgmembrane, natural
5026 0.45 µm BioTrace PVDF 10/pkgmembrane, black
5027 0.45 µm BioTrace PVDF 10/pkgmembrane, white
AcroPrep 384 Filter Plates, 100 µL WellPart Number Description Packaging
5076 10K Omega™ membrane, 10/pkglong tips, natural
5077 10K Omega membrane, 10/pkgshort tips, natural
5078 30K Omega membrane, 10/pkglong tips, natural
5079 30K Omega membrane, 10/pkgshort tips, natural
5080 100K Omega membrane, 10/pkglong tips, natural
5081 100K Omega membrane, 10/pkgshort tips, natural
5070 0.45 µm GHP membrane, 10/pkglong tips, natural
5071 0.45 µm GHP membrane, 10/pkgshort tips, natural
5072 1.0 µm glass fiber media, 10/pkglong tips, natural
5072W 1.0 µm glass fiber media, 10/pkglong tips, white
5073 1.0 µm glass fiber media, 10/pkgshort tips, natural
5073W 1.0 µm glass fiber media, 10/pkgshort tips, white
5084 1.2 µm Supor membrane, 10/pkglong tips, natural
5085 1.2 µm Supor membrane, 10/pkgshort tips, natural
Vacuum ManifoldPart Number Description Packaging
5017 Multi-well plate vacuum manifold 1/pkg
5014 1 mL receiver plate spacer block 1/pkg
5015 350 µL receiver plate 1/pkgspacer block
5016 Replacement accessory kit 1/pkg(includes O-ring, gasket,allen wrench)
AccessoriesPart Number Description Packaging
5225 Adapter collar for centrifugation 2/pkg
5230 96-well plate cap mats 5/pkg
5231 Multi-well plate lids 10/pkg
Centrifugal devices
Nanosep® Centrifugal Devices,Omega MembranePart Number Description Packaging
OD003C33 3K, gray 24/pkg
OD003C34 3K, gray 100/pkg
OD003C35 3K, gray 500/pkg
OD010C33 10K, blue 24/pkg
OD010C34 10K, blue 100/pkg
OD010C35 10K, blue 500/pkg
OD030C33 30K, red 24/pkg
OD030C34 30K, red 100/pkg
OD030C35 30K, red 500/pkg
OD100C33 100K, clear 24/pkg
OD100C34 100K, clear 100/pkg
OD100C35 100K, clear 500/pkg
OD300C33 300K, orange 24/pkg
OD300C34 300K, orange 100/pkg
OD300C35 300K, orange 500/pkg
Nanosep MF Centrifugal Devices,Bio-Inert MembranePart Number Description Packaging
ODM02C33 0.2 µm, aqua 24/pkg
ODM02C34 0.2 µm, aqua 100/pkg
ODM02C35 0.2 µm, aqua 500/pkg
ODM45C33 0.45 µm, wildberry 24/pkg
ODM45C34 0.45 µm, wildberry 100/pkg
ODM45C35 0.45 µm, wildberry 500/pkg
Nanosep MF Centrifugal Devices,GHP MembranePart Number Description Packaging
ODGHPC34 0.45 µm, clear 100/pkg
ODGHPC35 0.45 µm, clear 500/pkg
Microsep™ Centrifugal Devices,Omega MembranePart Number Description Packaging
OD001C41 1K, yellow 24/pkg
OD001C46 1K, yellow 100/pkg
OD003C41 3K, gray 24/pkg
OD003C46 3K, gray 100/pkg
OD010C41 10K, blue 24/pkg
OD010C46 10K, blue 100/pkg
OD030C41 30K, red 24/pkg
OD030C46 30K, red 100/pkg
OD050C41 50K, green 24/pkg
OD050C46 50K, green 100/pkg
OD100C41 100K, clear 24/pkg
OD100C46 100K, clear 100/pkg
OD300C41 300K, orange 24/pkg
OD300C46 300K, orange 100/pkg
OD990C41 1000K, purple 24/pkg
OD990C46 1000K, purple 100/pkg
Microsep MF Centrifugal Devices,Bio-Inert MembranePart Number Description Packaging
ODM02C67 0.2 µm, aqua 24/pkg
ODM02C68 0.2 µm, aqua 100/pkg
ODM45C67 0.45 µm, wildberry 24/pkg
ODM45C68 0.45 µm, wildberry 100/pkg
Macrosep® Centrifugal Devices,Omega MembranePart Number Description Packaging
OD001C36 1K, yellow 6/pkg
OD001C37 1K, yellow 24/pkg
OD001C38 1K, yellow 100/pkg
OD003C36 3K, gray 6/pkg
OD003C37 3K, gray 24/pkg
OD003C38 3K, gray 100/pkg
OD010C36 10K, blue 6/pkg
OD010C37 10K, blue 24/pkg
OD010C38 10K, blue 100/pkg
OD030C36 30K, red 6/pkg
OD030C37 30K, red 24/pkg
OD030C38 30K, red 100/pkg
† PTFE plates have polyester support.†† Glass Fiber plates have polypropylene support.
32
Ordering InformationCentrifugal devices
Macrosep® Centrifugal Devices,Omega™ MembranePart Number Description Packaging
OD050C36 50K, green 6/pkg
OD050C37 50K, green 24/pkg
OD050C38 50K, green 100/pkg
OD100C36 100K, clear 6/pkg
OD100C37 100K, clear 24/pkg
OD100C38 100K, clear 100/pkg
OD300C36 300K, orange 6/pkg
OD300C37 300K, orange 24/pkg
OD300C38 300K, orange 100/pkg
OD990C36 1000K, purple 6/pkg
OD990C37 1000K, purple 24/pkg
OD990C38 1000K, purple 100/pkg
Jumbosep™ Centrifugal Device Starter KitsPart Number Description Packaging
FD000K65 Generic starter kit, 4/pkg(no membrane inserts)
FD003K65 3K starter kit, gray 4/pkg
FD010K65 10K starter kit, blue 4/pkg
FD030K65 30K starter kit, red 4/pkg
FD100K65 100K starter kit, clear 4/pkg
FD300K65 300K starter kit, orange 4/pkg
Jumbosep Centrifugal Device Membrane InsertsPart Number Description Packaging
OD003C65 3K membrane insert, gray 12/pkg
OD010C65 10K membrane insert, blue 12/pkg
OD030C65 30K membrane insert, red 12/pkg
OD100C65 100K membrane insert, clear 12/pkg
OD300C65 300K membrane insert, orange 12/pkg
Jumbosep Device Accessory ProductsPart Number Description Packaging
FD001X65 Filtrate receiver and cap 12/pkg
FD002X65 Sample reservoir and cap 12/pkg
FD003X65 Insert release 24/pkg
Stirred cell systems
10 mL Omega Membrane-cell PacksPart Number Description Packaging
OC001C30 1K, yellow 10/pkg
OC003C30 3K, gray 10/pkg
OC005C30 5K, tan 10/pkg
OC010C30 10K, blue 10/pkg
OC030C30 30K, red 10/pkg
OC050C30 50K, green 10/pkg
OC100C30 100K, clear 10/pkg
OC300C30 300K, orange 10/pkg
150 mL Omega Membrane-cell PacksPart Number Description Packaging
OC001C60 1K, yellow 5/pkg
OC003C60 3K, gray 5/pkg
OC005C60 5K, tan 5/pkg
OC010C60 10K, blue 5/pkg
OC030C60 30K, red 5/pkg
OC050C60 50K, green 5/pkg
OC100C60 100K, clear 5/pkg
OC300C60 300K, orange 5/pkg
Stirred Cell Generic Starter KitsPart Number Description Packaging
FC000K30 10 mL Generic Starter Kit 1 kit
FC000K60 150 mL Generic Starter Kit 1 kit
Enchant™ protein purification kits
Enchant Life Science Kit Albumin DepletionPart Number Description Packaging
5300- Enchant Albumin Depletion 25 ALBDEP Kit (25 Nanosep® 0.45 µm samples
GHP centrifugal devices,25 Nanosep filtrate tubes, 25 albumin-depleting discs, 6.25 mL binding/wash buffer)
Enchant Life Science Kits IgG PurificationPart Number Description Packaging
5300- Enchant Protein A IgG 50IGGPROA Purification Kit (5 Protein A purifications
affinity purification columns,5 desalting columns, 1 liter Protein A binding buffer,500 mL Protein A elution buffer)
5300- Enchant Protein G IgG 10 IGGPROG Purification Kit (1 Protein G purifications
affinity purification column,5 desalting columns, 240 mL Protein G binding buffer,120 mL Protein G elution buffer)
Enchant Multi-Protein Affinity Separation KitPart Number Description Packaging
5300- Enchant Multi-Protein Affinity 24AFFMPS Separation Kit (24 Nanosep purifications
centrifugal devices, 5 mL anti-HSA resin, 5 mL anti-IgG resin, 20 mL wash buffer, 20 mL elution buffer)
Omega Membrane Discs (25, 43, 47, 50, 62, and 76 mm provided 12/pkg; 90 and 150 mm provided 6/pkg)MWCO 25 mm 43 mm 47 mm 50 mm 62 mm 76 mm 90 mm 150 mm1K OM001025 OM001043 OM001047 OM001050 OM001062 OM001076 OM001090 OM001150
3K OM003025 OM003043 OM003047 OM003050 OM003062 OM003076 OM003090 OM003150
5K OM005025 OM005043 OM005047 OM005050 OM005062 OM005076 OM005090 OM005150
10K OM010025 OM010043 OM010047 OM010050 OM010062 OM010076 OM010090 OM010150
30K OM030025 OM030043 OM030047 OM030050 OM030062 OM030076 OM030090 OM030150
50K OM050025 OM050043 OM050047 OM050050 OM050062 OM050076 OM050090 OM050150
100K OM100025 OM100043 OM100047 OM100050 OM100062 OM100076 OM100090 OM100150
300K OM300025 OM300043 OM300047 OM300050 OM300062 OM300076 OM300090 OM300150
www.pall.com33
*One Minimate TFF accessory kit is included in each Minimate TFF capsule package.
Tangential flowfiltration products
Minimate™ TFF Capsules,Omega™ MembranePart Number Description Packaging
OAD65C12 650D 1/pkg
OA001C12 1K 1/pkg
OA003C12 3K 1/pkg
OA005C12 5K 1/pkg
OA010C12 10K 1/pkg
OA030C12 30K 1/pkg
OA050C12 50K 1/pkg
OA070C12 70K 1/pkg
OA100C12 100K 1/pkg
OA300C12 300K 1/pkg
OA500C12 500K 1/pkg
OA990C12 1000K 1/pkg
Minimate TFF Capsule Accessory ProductPart Number Description Packaging
88216 Minimate Fitting Kit* 1/pkgConsists of male luer to 3.2 mm (1/8 in.) hose barb, female luer to 3.2 mm (1/8 in.) hose barb,3.2 mm (1/8 in.) i.d. tubing,tubing screw clamp, tubing clamps, adhesive strips (loop and hook)
Minimate TFF SystemsPart Number Description Packaging
OAPMP110 115V AC 50/60 Hz 1/pkg
OAPMP220 230V AC 50/60 Hz 1/pkg
OAPMP220UK 230V AC 50/60 Hz 1/pkgwith UK plug
Minimate TFF Reservoir AssemblyPart Number Description Packaging
OARES110 Reservoir Assembly 1/pkg115V AC 50/60 Hz
OARES220 Reservoir Assembly 1/pkg230V AC 50/60 HZ
OARES220UK Reservoir Assembly 1/pkg230V AC 50/60 HZ with UK plug
LV Centramate™ TFF Holder and AccessoriesPart Number Description Packaging
FS003K10 LV Centramate cassette holder 1/pkg
FS007X01 Bronze nuts and washers 4/pkg
FS710M01 Pressure gauge assembly 1/pkg[2 needed (filtrate and retentate); 3 needed if monitoring required]
FS700X10 Male:male connector to attach 8/pkgpressure gauges to holder
LV Centramate Cassettes, Alpha™ Membrane Medium Suspended
MWCO EFA Screen Screen
10K 0.01 m2 (0.1 ft2) AS010C12P1 AS010C11P1
10K 0.02 m2 (0.2 ft2) AS010C12P2 AS010C11P2
LV Centramate Cassettes, Supor® Membrane Medium Suspended
Pore Size EFA Screen Screen
0.1 µm 0.01 m2 (0.1 ft2) PSM10C12P1 PSM10C11P1
0.1 µm 0.02 m2 (0.2 ft2) PSM10C12P2 PSM10C11P2
0.2 µm 0.01 m2 (0.1 ft2) PSM20C12P1 PSM20C11P1
0.2 µm 0.02 m2 (0.2 ft2) PSM20C12P2 PSM20C11P2
0.45 µm 0.01 m2 (0.1 ft2) PSM45C12P1 PSM45C11P1
0.45 µm 0.02 m2 (0.2 ft2) PSM45C12P2 PSM45C11P2
0.65 µm 0.01 m2 (0.1 ft2) PSM65C12P1 PSM65C11P1
0.65 µm 0.02 m2 (0.2 ft2) PSM65C12P2 PSM65C11P2
0.8 µm 0.01 m2 (0.1 ft2) PSM80C12P1 PSM80C11P1
0.8 µm 0.02 m2 (0.2 ft2) PSM80C12P2 PSM80C11P2
LV Centramate Cassettes, Omega Membrane Medium Suspended
Pore Size EFA Screen Screen
1K 0.01 m2 (0.1 ft2) OS001C12P1 OS001C11P1
1K 0.02 m2 (0.2 ft2) OS001C12P2 OS001C11P2
3K 0.01 m2 (0.1 ft2) OS003C12P1 OS003C11P1
3K 0.02 m2 (0.2 ft2) OS003C12P2 OS003C11P2
5K 0.01 m2(0.1 ft2) OS005C12P1 OS005C11P1
5K 0.02 m2 (0.2 ft2) OS005C12P2 OS005C11P2
10K 0.01 m2 (0.1 ft2) OS010C12P1 OS010C11P1
10K 0.02 m2 (0.2 ft2) OS010C12P2 OS010C11P2
30K 0.01 m2 (0.1 ft2) OS030C12P1 OS030C11P1
30K 0.02 m2 (0.2 ft2) OS030C12P2 OS030C11P2
50K 0.01 m2 (0.1 ft2) OS050C12P1 OS050C11P1
50K 0.02 m2 (0.2 ft2) OS050C12P2 OS050C11P2
70K 0.01 m2 (0.1 ft2) OS070C12P1 OS070C11P1
70K 0.02 m2 (0.2 ft2) OS070C12P2 OS070C11P2
100K 0.01 m2 (0.1 ft2) OS100C12P1 OS100C11P1
100K 0.02 m2 (0.2 ft2) OS100C12P2 OS100C11P2
300K 0.01 m2 (0.1 ft2) OS300C12P1 OS300C11P1
300K 0.02 m2 (0.2 ft2) OS300C12P2 OS300C11P2
500K 0.01 m2 (0.1 ft2) OS500C12P1 OS500C11P1
500K 0.02 m2 (0.2 ft2) OS500C12P2 OS500C11P2
1000K 0.01 m2 (0.1 ft2) OS990C12P1 OS990C11P1
1000K 0.02 m2 (0.2 ft2) OS990C12P2 OS990C11P2
0.16 µm 0.01 m2 (0.1 ft2) OS994C12P1 OS994C11P1
0.16 µm 0.02 m2 (0.2 ft2) OS994C12P2 OS994C11P2
Centramate Cassette HoldersPart Number Description Packaging
FS001K10 Includes stainless steel 1/pkgCentramate cassette holder,assorted fittings, torque wrench, and socket
FS002K10 Includes polyethylene holder 1/pkgwith Centramate cassette,stainless steel top and bottom brace plates, assorted fittings,torque wrench, and socket
Centramate Sanitary Gauge Fitting PackagesPart Number Description Packaging
FS005K10 2-gauge fitting package consists 1/pkgof (8) 1/2 in. EPDM gaskets,(2) 1-1/2 in. EPDM gaskets,(2) 1/2 in. x 1-1/2 in. TC tees,(3) 1/2 in. TC to 1/4 in. ID tube barbed fittings, (8) 1/2 in. TC clamps, (1) 1/2 in. diaphragm valve, (1) filtrate manifold,(2) 0-60 PSIG glycerin-filled gauges, (3) 3/4 in. TC to 1/2 in.ID tube barbed fittings, and (2) 1-1/2 in. TC sanitary clamps
FS006K10 3-gauge fitting package consists 1/pkgof (10) 1/2 in. EPDM gaskets,(3) 1-1/2 in. EPDM gaskets,(3) 1/2 in. x 1-1/2 in. TC tees,(3) 1/2 in. TC to 1/4 in. ID tube barbed fittings, (13) 1/2 in. TC clamps, (2) 1/2 in. diaphragm valves, (1) filtrate manifold,(3) 0-60 PSIG glycerin-filled gauges, (3) 3/4 in. TC to 1/2 in.ID tube barbed fittings, and (3) 1-1/2 in. TC sanitary clamps
Centramate PE Fitting PackagesPart Number Description Packaging
FS007K10 2-gauge fitting package 1/pkgconsists of (2) 1/4 in. NPT nipples, (2) 1/4 in. NPT tees,(2) 1/4 in. NPT to 1/4 in.barbed fittings, (1) 1/4 in. NPT to 1/4 in. barbed elbow fitting,(2) PSIG glycerin-filled gauges,(1) 1/4 in. ID tubing, (2) 1/4 in.NPT to 1/2 in. barbed fittings,(5) stainless steel hose clamps,and (1) 1/4 in. NPT to 1/4 in.barbed tee fitting
FS008K10 3-gauge fitting package 1/pkgconsists of (4) 1/4 in. NPT nipples, (4) 1/4 in. NPT tees,(3) 1/4 in. NPT to 1/4 in.barbed fittings, (2) 1/4 in. NPT to 1/4 in. barbed elbow fittings,(3) PSIG glycerin-filled gauges,(1) 1/4 in. ID tubing, (2) 1/4 in.NPT to 1/2 in. barbed fittings,and (5) stainless steel hose clamps
34
Ordering InformationTangential flowfiltration products
Centramate™ Cassette Hardware SystemsPart Number Description Packaging
FS010K10 Centramate system, 1/pkg2-gauge includes PN FS001K10 and FS005K10
FS011K10 Centramate system, 1/pkg3-gauge includes PN FS001K10 and FS006K10
Centramate PE Cassette Hardware SystemsPart Number Description Packaging
FS012K10 Centramate PE system, 1/pkg2-gauge includes PN FS002K10 and FS007K10
FS013K10 Centramate PE system, 1/pkg3-gauge includes PN FS002K10 and FS008K10
Centramate Cassettes, Omega™ Membrane Fine Medium Suspended
MWCO Screen Screen Screen
1K OS001C10 OS001C12 OS001C11
3K OS003C10 OS003C12 OS003C11
5K OS005C10 OS005C12 OS005C11
10K OS010C10 OS010C12 OS010C11
30K OS030C10 OS030C12 OS030C11
50K OS050C10 OS050C12 OS050C11
70K OS070C10 OS070C12 OS070C11
100K OS100C10 OS100C12 OS100C11
300K N/A OS300C12 OS300C11
500K N/A OS500C12 OS500C11
1000K N/A OS990C12 OS990C11
0.16 µm N/A OS994C12 OS994C11
Centramate Cassettes, Supor® MembraneMedium Suspended
Pore Size Screen Screen
0.03 µm PSM03C12 PSM03C11
0.1 µm PSM10C12 PSM10C11
0.2 µm PSM20C12 PSM20C11
0.45 µm PSM45C12 PSM45C11
0.65 µm PSM65C12 PSM65C11
0.8 µm PSM80C12 PSM80C11
Centramate Cassettes, Alpha™ MembraneFine Medium Suspended
MWCO Screen Screen Screen
10K AS010C10 AS010C12 AS010C11
Ultrasette™ Device Packages,Omega Membrane
Suspended MWCO Screen Channel Screen
1K, yellow OS001C70 OS001C72
3K, gray OS003C70 OS003C72
5K, tan OS005C70 OS005C72
10K, blue OS010C70 OS010C72
30K, red OS030C70 OS030C72
50K, green OS050C70 N/A
70K, brown OS070C70 N/A
100K, clear OS100C70 OS100C72
300K, orange OS300C70 OS300C72
Device packages include (1) device in the MWCO of your choice, (2) storagecaps for feed/retentate, filtrate outlet cap,(2) tubing clamps, and 0.6 m (24 in.) of 4.8 mm (3/16 in.) tubing.
Ultrasette Device Accessory ProductsPart Number Description Packaging
FS002X70 Accessory kit consists of 1/pkg1.8 m (6 ft.) of PharMed* #24 feed/retentate tubing,0.6 m (24 in.) of 4.8 mm (3/16 in.) Tygon filtrate tubing, (8) stainless steel tubing clamps, (1) screw clamp, and (1) barbed fitting
FS005X70 Gauge fitting package 1/pkgconsists of (1) 0 - 4.1 bar (0 - 60 psi) 3.2 mm (1/8 in.) NPT pressure gauge,(2) 6.4 mm (1/4 in.) O.D.polypropylene barbed tube to 3.2 mm (1/8 in.) NPT connectors, (1) 3.2 mm (1/8 in.) threaded polypropylene tee,(1) screw clamp,(2) stainless steel tubing clamps
FS001X70 Mounting bracket, 1/pkgholds Ultrasette device securely during operation; suction cups on the bottom of the bracket allow for placement on smooth surfaces
Ultralab™ Systems with 115 V PumpPart Number Description Packaging
FS006X75 2 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex* L/S* variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately
FS007X70 5 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex L/S variable speed peristalic pump and Ultrasette accessory kit; connects to Ultrasette device sold separately
Ultralab Systems with 230 V PumpPart Number Description Packaging
FS016X75 2 L Ultralab system 1/pkgconsists of an Ultrareservoir container, Masterflex L/S variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately
FS017X70 5 L Ultralab system 1/pkgconsists of an Ultrareservoir container Masterflex L/S variable speed peristalic pump, and Ultrasette accessory kit; connects to Ultrasette device sold separately
Ultrareservoir™ ContainersPart Number Description Packaging
FS005X75 2 L container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with Ultrasette device
FS006X70 5 L container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with Ultrasette device; fittings supplied are 6.4 mm
FS007X75 500 mL container includes 1/pkg0 - 4.2 bar pressure gauge,4.0 mm fittings, 6.4 mm fittings,and 3-way valve; suitable for use with LV Centramate system
www.pall.com35
Prefiltration and clarification
Acrodisc® PF Syringe Filters,Supor MembranePart Number Description Packaging
4187 0.8/0.2 µm, 25 mm, sterile 50/pkg
4658 0.8/0.2 µm, 32 mm, sterile 50/pkg
Acrodisc PSF GxF Syringe Filter, Glass FiberPart Number Description Packaging
AP-4523 GXF/Glass, 25 mm, 50/pkg,non-sterile 200/cs
Serum Acrodisc Syringe Filter,Supor MembranePart Number Description Packaging
4525 GF/0.2 µm, 37 mm, sterile 20/pkg
Acrodisc Syringe Filter, Glass FiberPart Number Description Packaging
4524 1 µm (nominal), 37 mm, 15/pkg,non-sterile 60/cs
VacuCap® 60 PF Device,Supor MembranePart Number Description Packaging
4638 0.8/0.2 µm, 60 mm, sterile 10/pkg
VacuCap 90 PF Device,Supor MembranePart Number Description Packaging
4628 0.8/0.2 µm, 90 mm, sterile 10/pkg
Protein detection products
Biodyne® A MembranePart Number Description Packaging
60113 0.2 µm, 30 cm x 3 m roll 1/pkg
60102 0.45 µm, 82 mm discs 50/pkg
60103 0.45 µm, 85 mm discs 50/pkg
60104 0.45 µm, 132 mm discs 50/pkg
60105 0.45 µm, 137 mm discs 50/pkg
60101 0.45 µm, 7 x 8.5 cm sheets 10/pkg
60100 0.45 µm, 20 x 20 cm sheets 10/pkg
60120 0.45 µm, 20 cm x 3 m roll 1/pkg
60106 0.45 µm, 30 cm x 3 m roll 1/pkg
60108 1.2 µm, 30 cm x 3 m roll 1/pkg
Biodyne B Membrane, 0.45 µmPart Number Description Packaging
60202 82 mm discs 50/pkg
60203 85 mm discs 50/pkg
60204 132 mm discs 50/pkg
60205 137 mm discs 50/pkg
60201 7 x 8.5 cm sheets 10/pkg
60200 20 x 20 cm sheets 10/pkg
60209 20 cm x 1 m roll 1/pkg
60208 20 cm x 3 m roll 1/pkg
60207 30 cm x 3 m roll 1/pkg
Biodyne C Membrane, 0.45 µmPart Number Description Packaging
60316 82 mm discs 50/pkg
60317 85 mm discs 50/pkg
60318 132 mm discs 50/pkg
60319 137 mm discs 50/pkg
60315 7 x 8.5 cm sheets 10/pkg
60314 20 x 20 cm sheets 10/pkg
60251 29 cm x 3 m roll 1/pkg
Biodyne Plus Membrane, 0.45 µmPart Number Description Packaging
60402 82 mm discs 50/pkg
60403 85 mm discs 50/pkg
60404 132 mm discs 50/pkg
60405 137 mm discs 50/pkg
60401 7 x 8.5 cm sheets 10/pkg
60400 20 x 20 cm sheets 10/pkg
60406 30 cm x 3 m roll 1/pkg
BioTrace™ NT Nitrocellulose TransferMembranePart Number Description Packaging
66487 82 mm discs 50/pkg
66595 85 mm discs 50/pkg
66518 132 mm discs 50/pkg
66488 137 mm discs 50/pkg
66593 7 x 8.5 cm sheets 10/pkg
66489 20 x 20 cm sheets 10/pkg
66485 30 cm x 3 m roll 1/pkg
FluoroTrans® MembranePart Number Description Packaging
PVM020C-160 7 x 8.4 cm sheets 10/pkg
PVM020C-195 8.5 x 9 cm sheets 20/pkg
PVM020C1015 10 x 15 cm sheets 10/pkg
PVM020C-196 13 x 14 cm sheets 10/pkg
PVM020C2020 20 x 20 cm sheets 10/pkg
PVM020C-099 26 cm x 3.3 m roll 1/pkg
FluoroTrans W MembranePart Number Description Packaging
BSP0158 7 x 9 cm sheets 10/pkg
BSP0157 10 x 15 cm sheets 10/pkg
BSP0159 20 x 20 cm sheets 10/pkg
BSP0161 26 cm x 3.3 m roll 1/pkg
BioTrace PVDF Transfer MembranePart Number Description Packaging
66594 7 x 8.5 cm sheets 10/pkg
66542 20 x 20 cm sheets 10/pkg
66547 20 cm x 1 m roll 1/pkg
66543 30 cm x 3 m roll 1/pkg
UltraBind™ Affinity MembranePart Number Description Packaging
66544 20 x 20 cm sheets 10/pkg
66545 30 cm x 3 m roll 1/pkg
Immunodyne® ABC MembranePart Number Description Packaging
NBCH3RI 0.45 µm, 30 cm x 3 m roll 1/pkg
NNCH3RI 1.2 µm, 30 cm x 3 m roll 1/pkg
Vivid™ Gene Array SlidesPart Number Description Packaging
5110 Vivid Gene Array slides 10/pkgwith modified Nylon
5111 Vivid Gene Array slides 20/pkgwith modified Nylon
AcroWell™ 96 Filter Plates,BioTrace NT Membrane, 350 µLPart Number Description Packaging
5022 BioTrace NT membrane, white 10/pkg
5025 BioTrace NT membrane, black 10/pkg
AcroWell 96 Filter Plates,BioTrace PVDF Membrane, 350 µLPart Number Description Packaging
5023 BioTrace PVDF membrane, natural 10/pkg
5026 BioTrace PVDF membrane, black 10/pkg
5027 BioTrace PVDF membrane, white 10/pkg
Visit us on the Web at www.pall.com/lab
E-mail us at Lab@pall.com
Pall LIfe Sciences600 South Wagner RoadAnn Arbor, MI 48103-9019 USA
800.521.1520 toll free in USA734.665.0651 phone 734.913.6114 fax
On-demand Support & In-demand RewardsProteomics Scientific and Educational Resource Centerwww.pall.com/proteomics
Pall’s online resource center offers a complete library oftechnical resources, including technical protocols andproduct information, for all aspects of proteomic research.Visit us today to see how Pall Life Sciences can helpstreamline your proteomics workflow.
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© 2006 Pall Corporation. Pall, , Acrodisc, AcroPrep, AcroWell, Alpha, Biodyne, Bio-Inert, BioSepra,BioTrace, Centramate, Enchant, Fluorodyne, FluoroTrans, HyperCel, HyperD, HyperZ, Immunodyne, Jumbosep, Leukosorb, LoProdyne, Macrosep, Microsep, Minimate, Mustang, Nanosep, Omega, Pallflex, Repel, Spherodex, Supor, Trisacryl, Tuffryn, UltraBind, Ultralab, Ultrareservoir, Ultrasette, Ultrogel, VacuCap,Versapor, and Vivid are trademarks of Pall Corporation. ® indicates a registered trademark in the USA.
is a service mark of Pall Corporation. *DELFIA is a registered trademark of PerkinElmer, Inc. Triton is a registered trademark of Rohm & Haas Company. Coomassie is a registeredtrademark of Imperial Chemicals Industries, Ltd. PharMed is a registered trademark of Norton Company. L/S and Masterflex are registered trademarks of Cole Parmer Instrument Company. Cibacron is a registeredtrademark of CIBA-Geigy Ltd.
7/06, PDF, GN05.1256 V2 PN 33411
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