alpha amylase restriction map

7/24/2019 alpha amylase restriction map

http://slidepdf.com/reader/full/alpha-amylase-restriction-map 1/12

BioEdit version 7.2.5 (12/11/2013) Restriction Mapping Utility(c)1998, Tom Hall

gb|CP012712.1|:845921-847621 Geobacillus thermoglucosidasius strain DSM 2542, complete genome Restriction Map2/2/2016 3:08:52 PM1701 base pairs

Translations: none

Restriction Enzyme Map:

1 ATGAAAAAATGTGATGTAATGGCACAAGTAGAACAAAAGTTGAAAGAAATATATGGACAGGAATACAAAGAGGAATATGC 801 TACTTTTTTACACTACATTACCGTGTTCATCTTGTTTTCAACTTTCTTTATATACCTGTCCTTATGTTTCTCCTTATACG 80 TspDTI MnlI

81 AGTGGCATTCCGCTCTTTCATCGAGCGTTGGGAAGCAAAACAATGGCGAAAAAACGAGCCGCTGTCAGAGAAGAATGTTT 16081 TCACCGTAAGGCGAGAAAGTAGCTCGCAACCCTTCGTTTTGTTACCGCTTTTTTGCTCGGCGACAGTCTCTTCTTACAAA 160 BsmI BsrBI Hin4I MspA1I

Hin4ITspRI

BtsI

TspDTI

161 ATTTAATCACGTATGGTGACAGCATTTTTGAAAAAGGGAAACCAGCGTTAGAGACACTGCATCGATTTTTGAAGGAGCAA 240161 TAAATTAGTGCATACCACTGTCGTAAAAACTTTTTCCCTTTGGTCGCAATCTCTGTGACGTAGCTAAAAACTTCCTCGTT 240 MboII BsaAI HphI Hin4I BtsI TspRISfaNI Hin4I

Hin4I ClaI

Hin4I BsmAI

241 GTAGGCGACATCATTACAGATGTCCACTTGCTTCCGATGTTTCCATATACATCCGATGACGGTTTTTCAGTGACAGATTA 320241 CATCCGCTGTAGTAATGTCTACAGGTGAACGAAGGCTACAAAGGTATATGTAGGCTACTGCCAAAAAGTCACTGTCTAAT 320 Hpy8I FokI BstF5I Hin4I

TspRI

Hin4IFokI



TspDTI

321 TCGGAAAATTCATCCGAAACTTGGCGGTTGGGAACAAATTGAAGCGATGGCGGCGGATTATCGTCTCATGTTTGACTTTG 400

321 AGCCTTTTAAGTAGGCTTTGAACCGCCAACCCTTGTTTAACTTCGCTACCGCCGCCTAATAGCAGAGTACAAACTGAAAC 400 ApoI Hin4I MwoI EciI Tth111I

BstF5I BslI HpyF10VIBsmBI

Hin4I BsmAI

401 TCGCTAATCATATTTCGAAATCGAGCGAATGGTTTCAGCGGTATTTAGCGGACGATCCAAAATATGAGCATTATTTTATT 480401 AGCGATTAGTATAAAGCTTTAGCTCGCTTACCAAAGTCGCCATAAATCGCCTGCTAGGTTTTATACTCGTAATAAAATAA 480 BstBI XmnI MspA1I AlwI

TspGWI

481 CCGAAAGATCCGTCTTTTGATACAAGCAACGTCATTCGTCCGCGCACGACACCGTTGTTCCATGAGTATCAAGGGATTCA 560481 GGCTTTCTAGGCAGAAAACTATGTTCGTTGCAGTAAGCAGGCGCGTGCTGTGGCAACAAGGTACTCATAGTT

CCCTAAGT 560 AlwI TspDTI

BstYI

561 TCGGGTGAAAACGGCTTGGACAACATTTAGCGAAGATCAAATAGATTTGAATTTCCGGCATTTCCCTGTGCTATTAGAAA 640561 AGCCCACTTTTGCCGAACCTGTTGTAAATCGCTTCTAGTTTATCTAAACTTAAAGGCCGTAAAGGGACACGATAATCTTT 640 HphI BceAI MboII

ApoI

641 TGACCGATATTTTGCTTGAGTATGCGCATAGACACGGCACGAGCATCCGGTTAGATGCGATCGGCTTCATTTGGAAGGAA 720641 ACTGGCTATAAAACGAACTCATACGCGTATCTGTGCCGTGCTCGTAGGCCAATCTACGCTAGCCGAAGTAAACCTTCCTT 720

SmlI FspAI BpuEI BstF5I SfaNI BsiEIBbvI

TaqII FspI BssSI BsaWI TspDTI



FokI SfaNI PvuI

BceAI

721 TCTGGTACAAGCTGCATGCACTTGCCGCAAACGCATGCAATTATTCAGTTATGGCGCCTTCTTCTCGACTATTTTCAGCC 800721 AGACCATGTTCGACGTACGTGAACGGCGTTTGCGTACGTTAATAAGTCAATACCGCGGAAGAAGAGCTGATAAAAGTCGG 800 Cac8I MwoI MwoI NspI MboII Hpy188III

NspI HpyF10VI Cac8I BanI

SphI HpyF10VI KasI

SphI BsaHI

NarI

NlaIV

SfoI

BbeI

HaeII

801 GAACACGAATATCATTACAGAAACGAACGTGCCGCATGATGAAAATATTCGTTATTTTGGTAATGGGATGAACGAGGCGC 880801 CTTGTGCTTATAGTAATGTCTTTGCTTGCACGGCGTACTACTTTTATAAGCAATAAAACCATTACCCTACTTGCTCCGCG 880 XmnI TspDTI MnlI FokI

SspIBstF5I

881 ATATGGTTTACCAATTTTCTTTGCCGCCGCTCGTGTTATATACGTTTACGGTCCATCATTCTCGCAAGTTGACAGAGTGG 960881 TATACCAAATGGTTAAAAGAAACGGCGGCGAGCACAATATATGCAAATGCCAGGTAGTAAGAGCGTTCAACTGTCTCACC 960 NdeI Hpy8I BsrBI Hpy8IHincII

TspDTI BssSIHpy8I

961 GCGAAAACGATTGATAAAGTTTCCGACAGCGCTACGTATTTTAACTTTTTAGCGAGCCACGACGGAATCGGC



ATGCGTCC 1040961 CGCTTTTGCTAACTATTTCAAAGGCTGTCGCGATGCATAAAATTGAAAAATCGCTCGGTGCTGCCTTAGCCGTACGCAGG 1040 AfeI MmeI Cac8I HgaI

Cac8IHaeII

NspI

BsaAISphI

SnaBITspGWI

1041 GACAGAAGGGATTTTGACGGAAGAAGAGAAACAACTGCTTGTGGACAAAGTATTACAAAACGGTGGGAAAGTGTCCTATA 11201041 CTGTCTTCCCTAAAACTGCCTTCTTCTCTTTGTTGACGAACACCTGTTTCATAATGTTTTGCCACCCTTTCACAGGATAT 1120

BslI EarI TspGWI Hpy8I

MmeI MboII

MboII

1121 AAACGAACACGGATGGCAGCCAATCGGTGTATGAGCTGAATATTAATTATTTTGATGCGCTTATTAACAAAGGTGTCGAT 12001121 TTTGCTTGTGCCTACCGTCGGTTAGCCACATACTCGACTTATAATTAATAAAACTACGCGAATAATTGTTTC

CACAGCTA 1200 BstF5I BbvI SspI

TspGWI AseI

FokI SfaNI

1201 GTGACCGAGCAGCAGCAAGTTCAAAAAATGCTTGCAGCTCATGCGATTTTGTTATCGGTCATTGGTGTTCCGGCGATTTA 12801201 CACTGGCTCGTCGTCGTTCAAGTTTTTTACGAACGTCGAGTACGCTAAAACAATAGCCAGTAACCACAAGGCCGCTAAAT 1280 TaqII Cac8I BstAPI Hin4I

BbvI MwoI BbvI Hin4I

BbvI HpyF10VI

TaqII

1281 TTATCACTCTTTACTCGGTTCGCGCAATGATTATAAAGGGCTGGAACAATCGGGAATTCCGCGCCGGATAAA



TCGCGAAA 13601281 AATAGTGAGAAATGAGCCAAGCGCGTTACTAATATTTCCCGACCTTGTTAGCCCTTAAGGCGCGGCCTATTTAGCGCTTT 1360 Hin4I BsrDI Hpy188III

Hpy188IIIHin4I PsiI ApoI

NruI

EcoRI

1361 AACTGGAGTACAGCCAAATTGTTCGTGAACTACAAACGAATTCTCGGCGCTGCGCGATTTTCCGTGGATTGAAACGAATG 14401361 TTGACCTCATGTCGGTTTAACAAGCACTTGATGTTTGCTTAAGAGCCGCGACGCGCTAAAAGGCACCTAACTTTGCTTAC 1440 BsrI BpmI BbvI HaeII BsaJI

FokI EciI

TatI Eco57MI ApoI TspGWI BtgI

Hpy188III EcoRI

Hpy8I

1441 ATCGCCATCCGCCGCACACAATCGGCATTTTCCCCGTACGCCCCGCAGCAAGTGATCGAACTAGGCGATCACGTATTTGC 15201441 TAGCGGTAGGCGGCGTGTGTTAGCCGTAAAAGGGGCATGCGGGGCGTCGTTCACTAGCTTGATCCGCTAGTG

CATAAACG 1520 BsaBI BsiWI FauI BbvI BceAI BsaAI

BstF5I

1521 CGTAAAAAGAACGAACGAACAGACTGGCGACTCGATATATTTTGTTGTCAACGTGACACCAGCCGAAATTGCTGTCGATT 16001521 GCATTTTTCTTGCTTGCTTGTCTGACCGCTGAGCTATATAAAACAACAGTTGCACTGTGGTCGGCTTTAACGACAGCTAA 1600 MlyI HincII MwoI

PleI Hpy8IHpyF10VI

BsrI

1601 TAGGAGTAAGCGGGGAAGATTTATGGACGGGAGAAAAAATAAACAGATCGTTCCGGTTGCTTCCGTATCAGTTTGTGTGG 1680

1601 ATCCTCATTCGCCCCTTCTAAATACCTGCCCTCTTTTTTATTTGTCTAGCAAGGCCAACGAAGGCATAGTCAAACACACC 1680 FauI MboII TspGWI T



aqII Hin4IBsaWI

BsaXI

1681 GTGAAGGAGAAATTGTCTTAA 17011681 CACTTCCTCTTTAACAGAATT 1701 HphI

Restriction table:

Enzyme Recognition frequency Positions __________________________________________________________________________ AfeI AGC'GCT 1 991AlwI GGATCnnnn'n_ 2 449, 482

ApoI r'AATT_y 4 327, 610, 1335, 1399AseI AT'TA_AT 1 1164BanI G'GyrC_C 1 774BbeI G_GCGC'C 1 778BbvI GCAGCnnnnnnnn'nnnn_ 7 719, 1149, 1222, 1225, 1247,1397 1498BceAI ACGGCnnnnnnnnnnnn'nn_ 3 588, 691, 1505BpmI CTGGAGnnnnnnnnnnnnnn_nn' 1 1385BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 677BsaAI yAC'GTr 3 171, 996, 1513BsaBI GATnn'nnATC 1 1445BsaHI Gr'CG_yC 1 775

BsaJI C'CnnG_G 1 1423BsaWI w'CCGG_w 2 687, 1653BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 1679BsiEI CG_ry'CG 1 702BsiWI C'GTAC_G 1 1476BslI CCnn_nnn'nnGG 2 341, 1046BsmI GAATG_Cn' 1 86BsmAI GTCTCn'nnnn_ 2 206, 389BsmBI CGTCTCn'nnnn_ 1 389BsrI ACTG_Gn' 2 1368, 1549BsrBI CCG'CTC 2 93, 910BsrDI GCAATG_nn' 1 1312BssSI C'ACGA_G 2 679, 911BstAPI GCAn_nnn'nTGC 1 1241BstBI TT'CG_AA 1 416BstF5I GGATG_nn' 6 290, 331, 684, 873, 1138, 1446BstYI r'GATC_y 1 487BtgI C'CryG_G 1 1423BtsI GCAGTG_nn' 2 87, 215Cac8I GCn'nGC 5 737, 756, 1015, 1034, 1233ClaI AT'CG_AT 1 223EarI CTCTTCn'nnn_ 1 1059EciI GGCGGAnnnnnnnnn_nn' 2 389, 1439Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 1 1385

EcoRI G'AATT_C 2 1335, 1399FauI CCCGCnnnn'nn_ 2 1491, 1604FokI GGATGnnnnnnnnn'nnnn_ 6 277, 318, 671, 880, 1145, 143



3FspI TGC'GCA 1 666FspAI rTGC'GCAy 1 666HaeII r_GCGC'y 3 778, 993, 1411HgaI GACGCnnnnn'nnnnn_ 1 1025Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 8 128, 205, 237, 304, 336, 1267 1299, 1679

Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 7 160, 205, 237, 304, 336, 1267 1299HincII GTy'rAC 2 951, 1570HphI GGTGAnnnnnnn_n' 3 188, 577, 1693Hpy8I GTn'nAC 7 265, 889, 927, 951, 1084, 1388 1570Hpy188III TC'nn_GA 4 785, 1332, 1355, 1385HpyF10VI GCn_nnnnn'nGC 5 372, 746, 752, 1242, 1590KasI G'GCGC_C 1 774MboII GAAGAnnnnnnn_n' 6 163, 605, 773, 1073, 1076, 1628

MlyI GAGTCnnnnn' 1 1544MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 2 1008, 1064MnlI CCTCnnnnnn_n' 2 64, 868MspA1I CmG'CkG 2 142, 439MwoI GCnn_nnn'nnGC 5 371, 745, 751, 1241, 1589NarI GG'CG_CC 1 775NdeI CA'TA_TG 1 882NlaIV GGn'nCC 1 776NruI TCG'CGA 1 1356NspI r_CATG'y 3 739, 758, 1036PleI GAGTCnnnn'n_ 1 1544PsiI TTA'TAA 1 1314PvuI CG_AT'CG 1 702

SfaNI GCATCnnnnn'nnnn_ 4 229, 693, 685, 1165SfoI GGC'GCC 1 776SmlI C'TyrA_G 1 656SnaBI TAC'GTA 1 996SphI G_CATG'C 3 739, 758, 1036SspI AAT'ATT 2 847, 1162TaqII GACCGAnnnnnnnnn_nn' 3 659, 1220, 1246TaqII CACCCAnnnnnnnnn_nn' 1 1669TatI w'GTAC_w 1 1368TspDTI ATGAAnnnnnnnnn_nn' 7 17, 88, 320, 548, 697, 855, 884TspGWI ACGGAnnnnnnnnn_nn' 6 480, 1038, 1073, 1145, 1412,1653TspRI _nnCAsTGnn' 3 87, 222, 315Tth111I GACn'n_nGTC 1 398XmnI GAAnn'nnTTC 2 432, 846

Enzymes that cut five or fewer times

Enzyme Recognition frequency Positions __________________________________________________________________________ AfeI AGC'GCT 1 991AlwI GGATCnnnn'n_ 2 449, 482ApoI r'AATT_y 4 327, 610, 1335, 1399

AseI AT'TA_AT 1 1164BanI G'GyrC_C 1 774BbeI G_GCGC'C 1 778



BceAI ACGGCnnnnnnnnnnnn'nn_ 3 588, 691, 1505BpmI CTGGAGnnnnnnnnnnnnnn_nn' 1 1385BpuEI CTTGAGnnnnnnnnnnnnnn_nn' 1 677BsaAI yAC'GTr 3 171, 996, 1513BsaBI GATnn'nnATC 1 1445BsaHI Gr'CG_yC 1 775BsaJI C'CnnG_G 1 1423

BsaWI w'CCGG_w 2 687, 1653BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 1679BsiEI CG_ry'CG 1 702BsiWI C'GTAC_G 1 1476BslI CCnn_nnn'nnGG 2 341, 1046BsmI GAATG_Cn' 1 86BsmAI GTCTCn'nnnn_ 2 206, 389BsmBI CGTCTCn'nnnn_ 1 389BsrI ACTG_Gn' 2 1368, 1549BsrBI CCG'CTC 2 93, 910BsrDI GCAATG_nn' 1 1312BssSI C'ACGA_G 2 679, 911

BstAPI GCAn_nnn'nTGC 1 1241BstBI TT'CG_AA 1 416BstYI r'GATC_y 1 487BtgI C'CryG_G 1 1423BtsI GCAGTG_nn' 2 87, 215Cac8I GCn'nGC 5 737, 756, 1015, 1034, 1233ClaI AT'CG_AT 1 223EarI CTCTTCn'nnn_ 1 1059EciI GGCGGAnnnnnnnnn_nn' 2 389, 1439Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 1 1385EcoRI G'AATT_C 2 1335, 1399FauI CCCGCnnnn'nn_ 2 1491, 1604FspI TGC'GCA 1 666

FspAI rTGC'GCAy 1 666HaeII r_GCGC'y 3 778, 993, 1411HgaI GACGCnnnnn'nnnnn_ 1 1025HincII GTy'rAC 2 951, 1570HphI GGTGAnnnnnnn_n' 3 188, 577, 1693Hpy188III TC'nn_GA 4 785, 1332, 1355, 1385HpyF10VI GCn_nnnnn'nGC 5 372, 746, 752, 1242, 1590KasI G'GCGC_C 1 774MlyI GAGTCnnnnn' 1 1544MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 2 1008, 1064MnlI CCTCnnnnnn_n' 2 64, 868MspA1I CmG'CkG 2 142, 439MwoI GCnn_nnn'nnGC 5 371, 745, 751, 1241, 1589NarI GG'CG_CC 1 775NdeI CA'TA_TG 1 882NlaIV GGn'nCC 1 776NruI TCG'CGA 1 1356NspI r_CATG'y 3 739, 758, 1036PleI GAGTCnnnn'n_ 1 1544PsiI TTA'TAA 1 1314PvuI CG_AT'CG 1 702SfaNI GCATCnnnnn'nnnn_ 4 229, 693, 685, 1165SfoI GGC'GCC 1 776SmlI C'TyrA_G 1 656SnaBI TAC'GTA 1 996

SphI G_CATG'C 3 739, 758, 1036SspI AAT'ATT 2 847, 1162TaqII GACCGAnnnnnnnnn_nn' 3 659, 1220, 1246



TaqII CACCCAnnnnnnnnn_nn' 1 1669TatI w'GTAC_w 1 1368TspRI _nnCAsTGnn' 3 87, 222, 315Tth111I GACn'n_nGTC 1 398XmnI GAAnn'nnTTC 2 432, 846

Position Enzyme(s) __________________________________________________________________________ 17 TspDTI ATGAAnnnnnnnnn_nn'64 MnlI CCTCnnnnnn_n'86 BsmI GAATG_Cn'87 TspRI _nnCAsTGnn'87 BtsI GCAGTG_nn'88 TspDTI ATGAAnnnnnnnnn_nn'93 BsrBI CCG'CTC128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'142 MspA1I CmG'CkG160 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'

163 MboII GAAGAnnnnnnn_n'171 BsaAI yAC'GTr188 HphI GGTGAnnnnnnn_n'205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'205 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'206 BsmAI GTCTCn'nnnn_ 215 BtsI GCAGTG_nn'222 TspRI _nnCAsTGnn'223 ClaI AT'CG_AT229 SfaNI GCATCnnnnn'nnnn_ 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'237 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'265 Hpy8I GTn'nAC

277 FokI GGATGnnnnnnnnn'nnnn_ 290 BstF5I GGATG_nn'304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'304 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'315 TspRI _nnCAsTGnn'318 FokI GGATGnnnnnnnnn'nnnn_ 320 TspDTI ATGAAnnnnnnnnn_nn'327 ApoI r'AATT_y331 BstF5I GGATG_nn'336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'336 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'341 BslI CCnn_nnn'nnGG371 MwoI GCnn_nnn'nnGC372 HpyF10VI GCn_nnnnn'nGC389 EciI GGCGGAnnnnnnnnn_nn'389 BsmBI CGTCTCn'nnnn_ 389 BsmAI GTCTCn'nnnn_ 398 Tth111I GACn'n_nGTC416 BstBI TT'CG_AA432 XmnI GAAnn'nnTTC439 MspA1I CmG'CkG449 AlwI GGATCnnnn'n_ 480 TspGWI ACGGAnnnnnnnnn_nn'482 AlwI GGATCnnnn'n_ 487 BstYI r'GATC_y

548 TspDTI ATGAAnnnnnnnnn_nn'577 HphI GGTGAnnnnnnn_n'588 BceAI ACGGCnnnnnnnnnnnn'nn_



605 MboII GAAGAnnnnnnn_n'610 ApoI r'AATT_y656 SmlI C'TyrA_G659 TaqII GACCGAnnnnnnnnn_nn'666 FspAI rTGC'GCAy666 FspI TGC'GCA671 FokI GGATGnnnnnnnnn'nnnn_

677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn'679 BssSI C'ACGA_G684 BstF5I GGATG_nn'685 SfaNI GCATCnnnnn'nnnn_ 687 BsaWI w'CCGG_w691 BceAI ACGGCnnnnnnnnnnnn'nn_ 693 SfaNI GCATCnnnnn'nnnn_ 697 TspDTI ATGAAnnnnnnnnn_nn'702 BsiEI CG_ry'CG702 PvuI CG_AT'CG719 BbvI GCAGCnnnnnnnn'nnnn_ 737 Cac8I GCn'nGC

739 NspI r_CATG'y739 SphI G_CATG'C745 MwoI GCnn_nnn'nnGC746 HpyF10VI GCn_nnnnn'nGC751 MwoI GCnn_nnn'nnGC752 HpyF10VI GCn_nnnnn'nGC756 Cac8I GCn'nGC758 NspI r_CATG'y758 SphI G_CATG'C773 MboII GAAGAnnnnnnn_n'774 BanI G'GyrC_C774 KasI G'GCGC_C775 BsaHI Gr'CG_yC

775 NarI GG'CG_CC776 NlaIV GGn'nCC776 SfoI GGC'GCC778 BbeI G_GCGC'C778 HaeII r_GCGC'y785 Hpy188III TC'nn_GA846 XmnI GAAnn'nnTTC847 SspI AAT'ATT855 TspDTI ATGAAnnnnnnnnn_nn'868 MnlI CCTCnnnnnn_n'873 BstF5I GGATG_nn'880 FokI GGATGnnnnnnnnn'nnnn_ 882 NdeI CA'TA_TG884 TspDTI ATGAAnnnnnnnnn_nn'889 Hpy8I GTn'nAC910 BsrBI CCG'CTC911 BssSI C'ACGA_G927 Hpy8I GTn'nAC951 HincII GTy'rAC951 Hpy8I GTn'nAC991 AfeI AGC'GCT993 HaeII r_GCGC'y996 BsaAI yAC'GTr996 SnaBI TAC'GTA1008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

1015 Cac8I GCn'nGC1025 HgaI GACGCnnnnn'nnnnn_ 1034 Cac8I GCn'nGC



1036 NspI r_CATG'y1036 SphI G_CATG'C1038 TspGWI ACGGAnnnnnnnnn_nn'1046 BslI CCnn_nnn'nnGG1059 EarI CTCTTCn'nnn_ 1064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'1073 TspGWI ACGGAnnnnnnnnn_nn'

1073 MboII GAAGAnnnnnnn_n'1076 MboII GAAGAnnnnnnn_n'1084 Hpy8I GTn'nAC1138 BstF5I GGATG_nn'1145 TspGWI ACGGAnnnnnnnnn_nn'1145 FokI GGATGnnnnnnnnn'nnnn_ 1149 BbvI GCAGCnnnnnnnn'nnnn_ 1162 SspI AAT'ATT1164 AseI AT'TA_AT1165 SfaNI GCATCnnnnn'nnnn_ 1220 TaqII GACCGAnnnnnnnnn_nn'1222 BbvI GCAGCnnnnnnnn'nnnn_

1225 BbvI GCAGCnnnnnnnn'nnnn_ 1233 Cac8I GCn'nGC1241 BstAPI GCAn_nnn'nTGC1241 MwoI GCnn_nnn'nnGC1242 HpyF10VI GCn_nnnnn'nGC1246 TaqII GACCGAnnnnnnnnn_nn'1247 BbvI GCAGCnnnnnnnn'nnnn_ 1267 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'1267 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'1299 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'1299 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'1312 BsrDI GCAATG_nn'1314 PsiI TTA'TAA

1332 Hpy188III TC'nn_GA1335 ApoI r'AATT_y1335 EcoRI G'AATT_C1355 Hpy188III TC'nn_GA1356 NruI TCG'CGA1368 BsrI ACTG_Gn'1368 TatI w'GTAC_w1385 BpmI CTGGAGnnnnnnnnnnnnnn_nn'1385 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'1385 Hpy188III TC'nn_GA1388 Hpy8I GTn'nAC1397 BbvI GCAGCnnnnnnnn'nnnn_ 1399 ApoI r'AATT_y1399 EcoRI G'AATT_C1411 HaeII r_GCGC'y1412 TspGWI ACGGAnnnnnnnnn_nn'1423 BsaJI C'CnnG_G1423 BtgI C'CryG_G1433 FokI GGATGnnnnnnnnn'nnnn_ 1439 EciI GGCGGAnnnnnnnnn_nn'1445 BsaBI GATnn'nnATC1446 BstF5I GGATG_nn'1476 BsiWI C'GTAC_G1491 FauI CCCGCnnnn'nn_ 1498 BbvI GCAGCnnnnnnnn'nnnn_

1505 BceAI ACGGCnnnnnnnnnnnn'nn_ 1513 BsaAI yAC'GTr1544 MlyI GAGTCnnnnn'



1544 PleI GAGTCnnnn'n_ 1549 BsrI ACTG_Gn'1570 HincII GTy'rAC1570 Hpy8I GTn'nAC1589 MwoI GCnn_nnn'nnGC1590 HpyF10VI GCn_nnnnn'nGC1604 FauI CCCGCnnnn'nn_

1628 MboII GAAGAnnnnnnn_n'1653 TspGWI ACGGAnnnnnnnnn_nn'1653 BsaWI w'CCGG_w1669 TaqII CACCCAnnnnnnnnn_nn'1679 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'1679 BsaXI ACnnnnnCTCCnnnnnnn_nnn'1693 HphI GGTGAnnnnnnn_n'

Enzymes that do not cut: _________________________________________________________ AarI, AatII, AccI, Acc65I, AclI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI

AlwNI, ApaI, ApaLI, AscI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BanII, BbsIBbvCI, BcgI, BcgI, BciVI, BclI, BfrBI, BglI, BglII, BlpI, Bme1580I, BmgBI, BmrIBmtI, BplI, Bpu10I, BsaI, BsaXI, BseMII, BseRI, BseYI, BsgI, BsiHKAI, BsmFIBsp1286I, BspCNI, BspEI, BspHI, BspMI, BsrFI, BsrGI, BssHII, BstEII, BstXIBstZ17I, Bsu36I, DraI, DraIII, DrdI, EaeI, EagI, Eco57I, EcoICRI, EcoNI, EcoO109IEcoRV, FalI, FseI, HindIII, HpaI, KpnI, MfeI, MluI, MscI, MslI, NaeI, NcoI, NgoMIVNheI, NotI, NsiI, PacI, PciI, PflMI, PmeI, PmlI, PpiI, PpiI, PpuMI, PshAI, PspOMIPsrI, PsrI, PstI, PvuII, RsrII, SacI, SacII, SalI, SanDI, SapI, SbfI, ScaI, SexAISfcI, SfiI, SgrAI, SmaI, SpeI, SrfI, StuI, StyI, SwaI, XbaI, XcmI, XhoI, XmaI

ZraI

alpha amylase restriction map

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