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Alkaloids bio synthesis in Am aryllidacea e plant in vitro y ~ m

Vasil ' cor giev* I , Ivan Iva nov' , M ilen Georg iev ', Mladenka Ilieva' , Strahil Berko vo J.J! JAt nas Pavlov '

'Departme nt of M icrob ial Biosynthesis and Biotechno logies - La r.uory ill Plovdiv, The StephanAngelof f Institute of Mi crobiology , Bulgaria n Academy o f Sciences, 26 Maritza Blvd " 4002 Plovdiv,nd a Bulgaria, E-mai l: Jbpmhas@yah oo.comh ve

obiat2De partament de Productes Na tura ls, B iologia Vegeta l i Edafologia, Faeultat de Farmacia, Univers itatmos t de Barce lona , Av, Joan XXIII sin, 0802 8 Ba rce lo na, Spai n

ge s : "Presenti ng authorronhigh-

The plants of AmarylJidaceae family altracts a great attention due to the synthesis ofof speci fic alkaloids, with a wide range of bio logical and pharmacological activi ties such asx for

tide s AC hE inhib itory, citotoxic, an tiviral, an tifun gal among ot hers (Bas tida et al., 2006). One ofthem, galanthamine is a long acting AC hE inh ibi tor markete d for the treatment of

elop Alzheimer 's disease, Although chem ical syn the sis of galantharnine has been successfullyia ! inperfo rmed (Selles et al ., 199 7) , the ma in commercial sources for the production of thegalantharnine-based med icines are Arnaryllidaceae plan ts (Pav lov et al., 2007), Nowadays,prescription regime of the utilization of more of these plant species has been imposed and,from these perspectives, gala nthami ne production from in vitro cultures is consider ed andrug

te ly attrac tive alternative.or Leucojum aestivum L. (Amaryllidaceae), known as summer snowflake, is a threatened

plant speci es that is currently used as a commercial source of galantharnine in Bulgaria . It isgathered from the natural habitats for ind ustrial purposes, which causes inc reasing pro blemswith depletion of the wi ld populations.

Pancratium maritimum L. (sea daffodil) is a bul biferous plant belonging to the familyknot-93 Arnary llidaceac . It grows at ext reme enviro nmental conditions in sal ty and sandy soils alongfor the coastl ines of the Mediterranean, the Atlantic, the Black, and Caspian seas. Previous

ph ytochem ical studies resulted in identification of a num ber of bioactive alkaloids includingand ga lanthamine as we ll as several phenolic compounds ,ppl

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The lecture wi ll attend to both biotech approach for galanthamine production wi thLeucojum aestivum shoot culture and alka loid profili ng in Pancratioum maritimum in vitrocultures at d ifferent stages of differentiation - calli, sho ot-clumps and differentiated plantlets .

Acknow ledgments:Thi s research was supported by Bulgarian Science Fo und under contract TK -B

Re fere nces:

Bastida , J., Lavilla, R. and Yiladomat, F. (2006) Chapter 3 Chemical and Bio logical AspectsofNarcissus Alkaloids. Alkaloids: Chemistry and Biology. pp . 87-1 79.

Pavlov , A., Be rkov, S., Courot, E. , Gocheva, T., Tuneva , D., Pandova, B., Geo rgiev, M.,Georgiev, Y., Yaney , S., Burrus, M. and Ilieva, M . (2007) Galanthami ne productionby Leucoj um aestivurn in vitro systems. Process Biochemistry, 42 ,734 -739.

Selles, M., Bergonon , S., Vi ladornat, F., Bastida, J. and Codina , C. ( [997) Effect of sucroseon growth and galanthamine product ion in shoot-clump cultures ofNarcissus confususin liquid -shake medium . Plant Cell, Tissue and Organ Culture, 49, 129- 136.

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