adolescence of force feedback microscopy · system analysis of force feedback microscopy, journal...

17/03/2014 Luca Costa 1

Adolescence of Force Feedback Microscopy

L. Costa1,2, M. S. Rodrigues3, S. Carpentier4, M. Vitorino1,3, J.

Chevrier2,4,1, F. Comin1

1) European Synchrotron Radiation Facility, 6 rue Jules Horowitz BP 220, 38043 Grenoble

Cedex, France 2) UniversitéJoseph Fourier BP 53, 38041 Grenoble Cedex 9, France 3) CFMC/Dep. Fisica, Faculdade de Ciencias, Universidade de Lisboa, Campo Grande, 1749-

016 Lisboa,Portugal 4) Institut Néel CNRS BP 166, 38042 Grenoble Cedex 9, France

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OUTLINE • Force Feedback Microscope

• Interactions at the nanoscale

• Biomolecules

• Spectroscopy on living cells

• Optical beam deflection operational schemes

• Conclusions

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Introduction

Imaging modes

Optical beam deflection

Force Feedback Microscope:

an AFM where the total force acting on the tip is zero: Σi F

i = 0

An additional force Ffeedback

has to be applied

Ffeedback

= - Finteraction

Xt is kept constant

by a feedback loop

Force Feedback Microscope

Interaction curves

Elastic and inelastic interactions

Ffeedback

Finteraction

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AFM cantilever

Introduction

Imaging modes



Interaction curves


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Ffeedback

Finteraction

Introduction

Imaging modes



Interaction curves


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Approach – Retract force curves between a silicon nitride

probe and mica in deionized water

M. S. Rodrigues, L. Costa, J. Chevrier, and F. Comin, Applied Physics Letters 101, 203105 (2012)

Approach – Retract force curves between a silicon probe

and hydrophilic silicon native oxide surface in air

Introduction

Imaging modes



Interaction curves


Solid/liquid interface

Solid/air interface

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Introduction

Imaging modes



Interaction curves


Approach – Retract force curve between a silicon nitride probe and

mica in deionized water sample

M. S. Rodrigues, L. Costa, J. Chevrier, and F. Comin, Journal of Applied Physics 115, 054309(2014)

Force gradient & Dissipation

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Introduction

Imaging modes



Interaction curves


“Take-home” message - Σ

i F

i = 0 on the tip

- linear regime AFM with small oscillation amplitudes,

typically 1 – 2 Ångström → ɣ(d) and ∂zF(z)

- the frequency is arbitrarily chosen

- no jump to contact

M. S. Rodrigues, L. Costa, J. Chevrier, and F. Comin, Journal of Applied Physics 115, 054309(2014)

M. S. Rodrigues, L. Costa, J. Chevrier, and F. Comin, Applied Physics Letters 101, 203105 (2012)

17/03/2014 Luca Costa 9

Introduction

Imaging modes


Lipids

Living cells

(a) Topography

(b) Force

(c) Stiffness

(d) Dissipation

L. Costa, M. S. Rodrigues, E. Newman, C. Zubieta, J. Chevrier, and F. Comin, Journal of Molecular Recognition, 26: 689-693 (2013)

Phospholipids DSPE

Excitation frequency of the tip not linked

to resonance

Young modulus E ≈ 7 MPa

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Introduction

Imaging modes


Lipids

Living cells

Static indentation on living cells Quasi-static conditions: Young modulus ≈ 1 kPa

L. Costa, M. S. Rodrigues, N. Benseny-Cases, V. Mayeux, J. Chevrier, and F. Comin, in revision in PlosOne (2014)

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Introduction

Imaging modes


Lipids

Living cells

FFM indentation Quasi-static conditions: Young modulus ≈ 1 kPa

Blue = 1.13 kHz

Red = 5.13 kHz

Green = 7.13 kHz

Black = 11.13 kHz

L. Costa, M. S. Rodrigues, N. Benseny-Cases, V. Mayeux, J. Chevrier, and F. Comin, in revision in PlosOne (2014)

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Introduction

Imaging modes


Principle

Preliminary results

Black = No interaction

Red = cantilever base

inclined with interaction

Red dotted = cantilever

base inclined without

interaction

M. V. Vitorino, S. Carpentier, L. Costa, M. S. Rodrigues, in preparation (2014)

lf = illuminated cantilever length

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Introduction

Imaging modes


Principle

Preliminary results

Capillary condensation

M. V. Vitorino, S. Carpentier, L. Costa, M. S. Rodrigues, in preparation (2014)

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Introduction

Imaging modes


Conclusions

- F, ɣ(d) and ∂zF(z)

simultaneously

- the frequency is

arbitrarily chosen

- no jump to contact

Interferometric operational

schemes


operational schemes

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Surface Science Lab. Team

Fabio Comin

Joel Chevrier

Mario S. Rodrigues

..... and you for your kind attention

Introduction

Imaging modes

Living cells

Viscoelasticity vs frequency

Viscoelasticity: a local information

Conclusions

Simon Carpentier

Miguel V. Vitorino

Why do atomic force microscopy force curves still exhibit jump to contact? , Applied Physics Letters 101, 203105 (2012)

System analysis of force feedback microscopy, Journal of Applied Physics 115, 054309 (2014)

Imaging material properties of biological samples with a force feedback microscope, Journal of Molecular Recognition, 26: 689-693 (2013)

Spectroscopic investigation of local mechanical impedance of living cells, in revision in PlosOne (2014), arXiv:1310.6201

Force feedback Microscopy on a laser beam deflection scheme, Brownian motion and capillary bridges, in preparation (2014)

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Additional Slides

Images with a commercial AFM (Asylum Cypher)

Mica in water

Bacteriorhodopsin

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DMASP

Additional Slides

Digital Instruments Dimension 3100

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Additional Slides

Biomolecules images with a commercial AFM (Asylum MFP3D)

LIPIDS DNA Proteins

TBK1 OPTN

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Additional Slides

Tip power spectral density in air and liquid as a function of the tip-

fiber distance

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Additional Slides

The Force Feedback Microscope

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Additional Slides

Another FFM image on PC12 living cells

adolescence of force feedback microscopy · system analysis of force feedback microscopy, journal...

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