active transport in microtubules...
TRANSCRIPT
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Active Transport in Microtubules Networks
Rony GranekBiotechnology Engineering, BGU
Coworkers: Aviv Kahana, Gilad Kenan, Mario Feingold
BGUMichael Elbaum
WIS
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An idealized animal cell
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The cytoskeleton
http://img.sparknotes.com/figures/D/d479f5da672c08a54f986ae699069d7a/cytoskeleton.gif http://campus.queens.edu/faculty/jannr/cells/cell/pics/cytoskeleton.jpg%20
Microtubules are directional: (-) ends originate from the centrosome (MTOC)
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Motor proteins
• Kinesin moves toward (+) end, Dynein toward (-) end.
• Low processivity, ~1 s in bound state (step time ~6ms).
• Velocity ~1µm/s.
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Microtubules
Global order vs. local disorder
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Questions:
• What is the purpose of the finite motor processivity?
• What is the effect of local disorder of the microtubule network on the active transport in the cell?
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In vitro experiment: 3-D with orientational order
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In vitro study
• Fluorescently labeled ssDNA-protein complex including a nuclear localization signal (NLS) peptide. Motor protein assisted transport.
• Particle tracking assays using a camera & designated software.
H. Salman, A. Abu-Arish, S. Oliel, A.
Loyter, J. Klafter, R. Granek, and
M. Elbaum,
Biophys. J. (2005)
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Results
• pl – labeled complex without NLS
• an – labeled complex with NLS
• an+Noc – labeled complex with NLS without microtubules (destroyed by Nocodazole)
Question:
Why does the active transport appear as simple diffusion?
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Random velocity model in 1+1 dimensions
Diffusion
Act
ive
tran
sport
y
x
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2/32~)( tty
Simulation results:
• Balanced tracks (% up=% down) – fits the theory of ZKB.
• diffusion with drift – a crossover from short-time super-diffusion
to long-time diffusion
explained by a scaling argument
Random velocity model in 1+1 dimensions
2/32~)( tty
diffusion:-Exact result, Super
G. Zumofen, J. Klafter, A. Blumen, PRA (1990)S. Redner, PRE (1997)J.-P. Bouchaud, A. Georges, P. Le Doussal, J. Physique (1987)
Scaling argument:
22
,0
2)()( tvtPty
x where
2/1
,0~)(
ttP
x
The probability of return to the origin in 1-D
tty ~)(2
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MSD
Drift
define:
When >>1 RVM, when <<1 Diffusion
For p=0.51 & q=0.49: =1 at t=2500
0
2/12
/2 tpqx
0
tqpx
0
2/12
/
12
tqp
pq
x
x
RVM – Unbalanced diffusion
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2-D network model
22
,0
2)()( tvtPtx
y where
)(
1
2,0
ty
tPy
By symmetry
2
)()()(
2
22t
tytx
3/42~)( tt
Scaling argument:
The probablity of return to the origin along the y axis,assuming Gaussian PDF
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consistent calculation:-More accurate self
From symmetry
Assuming Gaussian PDFs
3/43/23/4
3/1
3/2
222 9tvyx
22yx
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2-D network model
3/422~ tyx
vtqpx
ty ~2
consistent theory:-Self
Simulation results:
• Balanced network – fits theory
•Unbalanced network, long times:
unbalanced direction RVM with drift
perpendicular direction Long-time diffusion
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Slope=1.25 (4/3)
Slope=1.02
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Slope=1.33
Slope=1.45
(3/2)
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f-Processivities
Slope=1.33
Processivity dependence
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3-D network model
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consistent calculation:-More accurate self
Scaling argument:
22
,0
2)()( tvtPtx
yz where
The probablity of return to the origin in the y-z plane
)(
1
)(
1)()(
22,0,0,0
tzty
tPtPtPzyyz
By symmetry
3
)()()()(
2
222tr
tztytx
ttr ~)(2
1/2
2ln
vτ
ttvAtr
4.2A
thermal)-like, but active (non-Diffusion
where
vv
is the mesh size,
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Slope=0.96
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In vitro experiment: 3-D with orientational order
zx
y
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In vitro study
• Fluorescently labeled ssDNA-protein complex including a nuclear localization signal (NLS) peptide. Motor protein assisted transport.
• Particle tracking assays using a camera & designated software.
H. Salman, A. Abu-Arish, S. Oliel, A.
Loyter, J. Klafter, R. Granek, and
M. Elbaum,
Biophys. J. (2005)
![Page 26: Active Transport in Microtubules Networksonline.itp.ucsb.edu/online/biopoly11/granek/pdf/Granek_Biopolymers... · y 2 (t) ~ t3 / 2 Simulation results: •Balanced tracks (% up=% down)](https://reader035.vdocuments.mx/reader035/viewer/2022071214/6043456d3b366b2f5b0c2918/html5/thumbnails/26.jpg)
Results
• pl – labeled complex without NLS
• an – labeled complex with NLS
• an+Noc – labeled complex with NLS without microtubules (destroyed by Nocodazole)
Question:
Why does the active transport appear as simple diffusion?
![Page 27: Active Transport in Microtubules Networksonline.itp.ucsb.edu/online/biopoly11/granek/pdf/Granek_Biopolymers... · y 2 (t) ~ t3 / 2 Simulation results: •Balanced tracks (% up=% down)](https://reader035.vdocuments.mx/reader035/viewer/2022071214/6043456d3b366b2f5b0c2918/html5/thumbnails/27.jpg)
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consistent calculation:-More accurate self
Scaling argument:
22
,0
2)()( tvtPtx
yz where
The probablity of return to the origin in the y-z plane
Dtty
tPtPtPzyyz
1
)(
1)()(
2,0,0,0
By symmetry )(,0,0
tPtPxzyz
and
2
)()()(
2
22t
tytx
tt ~)(2
3/2
3/1
3/4
2ln
vτ
tt
D
vAt
2.1A
thermal)-like, but active (non-Diffusion
where
vv
is the mesh size,
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Slope=0.63
3/2]
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-50-40
-30-20
-100
1020
3040
50
-50
-40
-30
-20
-10
0
10
20
30
40
50
-50
-40
-30
-20
-10
0
10
20
30
40
50
3-D animal cell model
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Simulations of “First Exit” problem:
• Kinesin mediated transport:
(i) Probability to arrive from the nucleus to the membrane until time t.
(ii) Probability to arrive from the nucleus to
a localized target in the cell (e.g., ribosome)
until time t.
• Dynein mediated transport: Probability to arrive from the membrane to the nucleus until time t.
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Kinesin mediated transport: From nucleus to membraneMany cells averaging
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Dynein mediated transport: From membrane to nucleus
Many cells averaging
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Kinesin mediated transport:
From nucleus to a localized target (e.g. ribosome)Radiative boundary conditions at the membrane
Many cells averaging
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Kinesin mediated transport:
From nucleus to a localized target (e.g. ribosome)Reflective boundary conditions at the membrane
Many cells averaging
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Short times
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What else?
Unusual Response to Force (?)
f
Assumption –
Linear-like response of a single motor walking on a single MT:
or
i.e. stall force is
fvv
0
fvv 0
0
stall
vf
mobility -
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Along the force:
tfx
*2/3
*3/4
2
for
for~
ttt
tttxx
Perpendicular to the force:
*
*3/4
2
for
for~
ttt
ttty
33
2
0*
f
vt
linear response
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Conclusions:
•Increase of polarity (velocity) field and Euclidean dimensions
leads to a decrease of the anomalous diffusion exponent.
•In 3-D disordered networks active transport may appear
diffusive-like (with minor logarithmic factors hinting to its
origin) consistent with experiments.
• The finite, intermediate, processivity of the microtubule
associated motor proteins appears “optimize” the efficiency of
transport between the different network tasks: transport from
nucleus to the membrane and vice-versa, and between
localized cell compartments.
• The local disorder of the microtubule network in the cell also
appears to enhance the efficiency of transport between
different locations.
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Thank you
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Slope=1.50
Slope=1.01
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Slope=1.333