acqknowledge 4 software guide

Upload: barbiegirl93

Post on 06-Jul-2018

260 views

Category:

Documents


0 download

TRANSCRIPT

  • 8/17/2019 Acqknowledge 4 Software Guide

    1/565

     

    Acq Knowledge® 4 Software Guide

    Check BIOPAC.COM > Support > Manuals for updates 

    For Life Science Research Applications

    Data Acquisition and Analysis with BIOPAC Hardware Systems

    Reference Manual for

    Acq Knowledge®

     4.4.1 Software & MP150/MP36R, BioHarness, B-Alert, Mobita, or StellarHardware/Firmware

    on Windows® 10, 8, and 7 or Mac OS® X 10.7-10.10

    42 Aero Camino, Goleta, CA 93117

    Tel (805) 685-0066 | Fax (805) 685-0067

    [email protected] | www.biopac.com

    http://www.biopac.com/LabManuals.asphttp://www.biopac.com/LabManuals.asphttp://www.biopac.com/http://www.biopac.com/http://www.biopac.com/LabManuals.asp

  • 8/17/2019 Acqknowledge 4 Software Guide

    2/565

    2   AcqKnowledge 4 Software Guide 

    Visit the online support center at www.biopac.com

    TABLE OF CONTENTS 

    PREFACE TO ACQKNOWLEDGE  SOFTWARE GUIDE .................................................. 15 Welcome ..................................................................................................................................................................15 

    Supported Platforms .................................................................................................................................................15 

    What’s new for Acq Knowledge 4.4.1 ......................................................................................................................16 

    User Support System ................................................................................................................................................19 

    Where do I find help? ...............................................................................................................................................20 

    PART A — GETTING STARTED ....................................................................................... 23 

    Chapter 1  MP Systems Overview ............................................................................................................................... 23 

    MP36R support ........................................................................................................................................................24 

    MP System Requirements ........................................................................................................................................24 

    Automator Integration and Scripting Support ..........................................................................................................25 

    MP System Features.................................................................................................................................................26 

    Application Features ................................................................................................................................................29 

    Application Notes ....................................................................................................................................................30 

    Chapter 2  Acq Knowledge Overview ........................................................................................................................... 31 

    Launching the Acq Knowledge software ..................................................................................................................32 

    Setting up channels using Module Setup (MP150 Hardware) .................................................................................34 Setting up channels manually...................................................................................................................................34 

    Basic Analog Channel Information ..........................................................................................................................35 

    Basic Digital Channel Information ..........................................................................................................................35 

    Basic Calculation Channel Information ...................................................................................................................35 

    Selecting Hardware ..................................................................................................................................................35 

    Setting Up Acquisitions ...........................................................................................................................................36 

    Starting an Acquisition .............................................................................................................................................37 

    Stopping an Acquisition ...........................................................................................................................................37 

    Display Modes .........................................................................................................................................................38 

    Data Views ...............................................................................................................................................................44 

    Analysis ...................................................................................................................................................................45 

    Selecting a waveform ...............................................................................................................................................47 Show/Hide Channel .................................................................................................................................................47 

    Collapsing Channels ................................................................................................................................................48 

    Zoom ........................................................................................................................................................................49 

    Select an area ...........................................................................................................................................................49 

    Keyboard data selection ...........................................................................................................................................49 

    Transform data .........................................................................................................................................................50 

    Measurements ..........................................................................................................................................................50 

    Events (Markers) ......................................................................................................................................................51 

    Grids .........................................................................................................................................................................51 

    Horizontal Split View ..............................................................................................................................................51 

  • 8/17/2019 Acqknowledge 4 Software Guide

    3/565

    Table of Contents  3

    AcqKnowledge 4 Software Guide 

    Autoscroll Horizontal Axis Controls ....................................................................................................................... 52 

    Journals .................................................................................................................................................................... 53 

    Saving data ............................................................................................................................................................... 53 

    Format change warnings ............................................................................................................................................ 53 

    “Data Snapshot” — Embedded Archive .................................................................................................................. 54 

    Print.......................................................................................................................................................................... 55 

    Chapter 3  User Interface & Context Menu Features ............................................................................................... 56 

    Toolbars ............................................................................................................................................................................. 57 

    Main, Display and Scaling Toolbars ........................................................................................................................ 58 

    Hardware Toolbar .................................................................................................................................................... 58 

    Cursor Toolbar ......................................................................................................................................................... 58 

    Selection Palette ....................................................................................................................................................... 62 

    Start/Stop Toolbar .................................................................................................................................................... 62 

    Channel Button Toolbar ........................................................................................................................................... 63 

    Event Toolbar .......................................................................................................................................................... 63 

    Focus Area Toolbar ................................................................................................................................................. 63 

    Measurements Toolbar............................................................................................................................................. 63 

    Custom Toolbars for Transformations and Analysis .......... .......... ........... ........... .......... ........... .......... ........... .......... . 63 

    Toolbar Position Retention and Changes ................................................................................................................. 64 Axis Controls ........................................................................................................................................................... 64 

    Enable Cursor Tools During Acquisitions ............................................................................................................... 64 

    Button Transparency ................................................................................................................................................ 65 

    Customizable Chart Track Dividers ......................................................................................................................... 65 

    Plotting Background Colors ..................................................................................................................................... 65 

    Spectrum Analyzer Palette ....................................................................................................................................... 65 

    Keyboard Shortcuts .................................................................................................................................................. 65 

    Tooltips .................................................................................................................................................................... 69 

    Mouse Controls ........................................................................................................................................................ 70 

    Mouse Scrollwheel Support ..................................................................................................................................... 70 

    Modification History ................................................................................................................................................ 70 

    Cancelling Transformations and Transformation Progress Bar ............................................................................... 71 Typed Event Label Drawing Improvements ............................................................................................................ 71 

    Choose MP150 Help Button .................................................................................................................................... 71 

    Tooltips .............................................................................................................................................................................. 71 

    Channel Label, Units Length and Tooltips ........... .......... ........... .......... ........... ........... .......... ........... .......... ........... .... 71 

    Graph Window Tooltip Improvements .................................................................................................................... 72 

    Menu Item Tooltips ................................................................................................................................................. 72 

    Chapter 4  Editing and Analysis Features .................................................................................................................. 73 

    Scroll bars ................................................................................................................................................................ 73 

    Scaling ..................................................................................................................................................................... 74 Horizontal axis ........................................................................................................................................................... 74 

    Vertical (Amplitude) axis .......................................................................................................................................... 75 Adaptive Scaling ...................................................................................................................................................... 76 

    Show Textual Value Display ................................................................................................................................... 76 

    Grid Details .............................................................................................................................................................. 78 Grid Options .............................................................................................................................................................. 81 

    Journal Details ......................................................................................................................................................... 83 

    Journal Contextual Menu ......................................................................................................................................... 83 

    Rich Journals.............................................................................................................................................................. 84 

    Journal Toolbar Buttons ............................................................................................................................................. 84 

    Journal Numerical Table Tools .................................................................................................................................. 85 

    Example of Sum, Mean or Standard .......................................................................................................................... 86 

    Example of Evaluate Expression ............................................................................................................................... 87 

  • 8/17/2019 Acqknowledge 4 Software Guide

    4/565

    4   AcqKnowledge 4 Software Guide 

    Visit the online support center at www.biopac.com

    Adding a hyperlink to the Journal .............................................................................................................................. 87 

    Embedding PDFs in Journals ..................................................................................................................................... 88 

    Select a waveform / channel .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........ 88 

    Channel Labels .........................................................................................................................................................88 

    Show/Hide Channel .................................................................................................................................................89 

    Focus Areas ..............................................................................................................................................................89 

    Creating Focus Areas ...............................................................................................................................................89 

    Measurements ..........................................................................................................................................................91 Measurement Display ................................................................................................................................................ 92 Measurement Area ..................................................................................................................................................... 92 

    Measurements and Measurement Presets ................................................................................................................... 94 

    Measurement Validation ............................................................................................................................................ 94 

    Measurement Info / Parameters ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ... 94 Measurement Interpolation ........................................................................................................................................ 95 

    Exporting measurements ......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .... 95 

    PART B—ACQUISITION FUNCTIONS: THE HARDWARE MENU ................................ 106 Overview ................................................................................................................................................................106 

    Set Up Data Acquisition > Data Acquisition Settings ...........................................................................................107 

    Chapter 5  Set Up Channels ....................................................................................................................................... 109 

    Set Up Channels—The Basics ........................................................................................................................................ 109 

    Module-based analog channel setup ......................................................................................................................109 

    View by Channels ..................................................................................................................................................111 

    Set Up Channels—Advanced ......................................................................................................................................... 114 

    Analog channels .....................................................................................................................................................114 

    Increased Channel Count Support (Acq Knowledge  4.3 and above) ......................................................................115 

    Analog channels MP36R .......................................................................................................................................116 

    Offset .....................................................................................................................................................................116 

    Adjustable, user defined, digital IIR filters for MP36R .........................................................................................117 

    High Pass Filters MP36R .......................................................................................................................................117 

    Additional controls in MP36R Input Channel Parameters .....................................................................................118 

    MP36R Advanced Preset Settings .........................................................................................................................118 

    MP36R Transducer SSID Table .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .........121 

    Digital Channels .....................................................................................................................................................122 

    Calculation Channels .............................................................................................................................................122 

    Metachannel ...........................................................................................................................................................123 

    Chapter 6  Calculation Channel Presets .................................................................................................................... 126 

    Integrate Calculation .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... ....... 127 

    Smoothing Calculation ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... .......... .... 132 

    Rate Calculation ....................................................................................................................................................... 134 Signal Parameters Tab ............................................................................................................................................. 135 

    Output Tab ............................................................................................................................................................... 136 Function Calculation .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... ....... 138 

    Filter IIR Calculation ............................................................................................................................................... 139 

    Expression ................................................................................................................................................................ 141 Delay Calculation ..................................................................................................................................................... 148 

    Fourier Linear Combiners: FLC, WFLC, CWFLC Calculations ............................................................................. 153 

    Basic FLC ................................................................................................................................................................ 153 

    Weighted-Frequency FLC ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... ......... 153 

    Coupled WFLC/FLC................................................................................................................................................ 153 

    Adaptive Filtering Calculation ................................................................................................................................. 154 Comb Band Stop Filter Calculation ......................................................................................................................... 154 

    Metachannel ............................................................................................................................................................. 154 

    Rescale Calculation .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... ......... 155 

  • 8/17/2019 Acqknowledge 4 Software Guide

    5/565

    Table of Contents  5

    AcqKnowledge 4 Software Guide 

    Slew Rate Limiter .................................................................................................................................................... 156 

    Filter - FIR ............................................................................................................................................................... 157 

    Chapter 7  Data Acquisition ...................................................................................................................................... 158 

    Data Acquisition—The Basics ............................................................................................................................... 158 

    Multiple Hardware ................................................................................................................................................... 161 

    Averaging (MP150 Hardware only) .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .. 162 

    Overview .................................................................................................................................................................. 162 

    Averaging Setup ...................................................................................................................................................... 164 Advanced Averaging—P300 ................................................................................................................................... 165 

    Repeating ................................................................................................................................................................. 167 

    Starting an acquisition............................................................................................................................................ 169 

    Stopping an Acquisition ......................................................................................................................................... 169 

    Rewind ................................................................................................................................................................... 169 

    Saving acquisition data .......................................................................................................................................... 169 

    Timers (Stop watches and Elapsed timers) ............................................................................................................ 170 

    Timer Types ........................................................................................................................................................... 170 

    Electrode Checker .................................................................................................................................................. 171 

    Chapter 8  Set Up Triggering ..................................................................................................................................... 173 

    Digital Triggers (MP150 only) .............................................................................................................................. 173 

    Analog Triggers ..................................................................................................................................................... 174 

    Chapter 9  Set Up Stimulator..................................................................................................................................... 176 

    Analog Output for MP150 Users ........................................................................................................................... 180 

    Dual Stimulation .................................................................................................................................................... 181 

    Square waves ......................................................................................................................................................... 182 

    Tone Stimuli .......................................................................................................................................................... 183 

    Ramp Waves .......................................................................................................................................................... 183 

    Arbitrary Waveform............................................................................................................................................... 184 

    MP150 Stimulator Sample Rates ........................................................................................................................... 185 

    Analog Output Upper Limits Summary ................................................................................................................. 186 

    Chapter 10  Output Control ..................................................................................................................................... 187 CH# to Output ........................................................................................................................................................ 189 

    MP36R Input > Output Scaling .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... ......... 190 

    Digital Outputs Control.......................................................................................................................................... 191 

    Pulses Output Control ............................................................................................................................................ 192 

    Stimulator – BSLSTM Output Control .................................................................................................................. 192 

    Stimulator – Low Voltage Output Control .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .... 192 

    Stimulator Human Stimulator (STMHUM) Output Control .................................................................................. 192 

    Pulse Sequence Output Control ............................................................................................................................. 194 

    Visual Stim Controllable LED – OUT4 Output Control .......... ........... .......... ........... .......... ........... .......... ........... .... 198 

    Sound Sequence Output Control ............................................................................................................................ 199 

    Pulse Defititions ..................................................................................................................................................... 201 

    Output Control Panel Descriptions ........................................................................................................................ 202 Usage Guidelines & Setup Summary for BSLSTM Output Control .............. ........... .......... ........... .......... ........... .. 211 

    Chapter 11  Set Up Event Marking......................................................................................................................... 213 

    Events (Markers) .................................................................................................................................................... 213 Event (Marker) Overview ........................................................................................................................................ 213 

    Event Toolbar .......................................................................................................................................................... 214 

    Event Tooltips .......................................................................................................................................................... 214 

    Preferences for Events ............................................................................................................................................. 214 Event Marking Setup ............................................................................................................................................... 216 

    Create/Toggle Focus Area Action............................................................................................................................ 217 

    Event Palette ............................................................................................................................................................ 218 

  • 8/17/2019 Acqknowledge 4 Software Guide

    6/565

    6   AcqKnowledge 4 Software Guide 

    Visit the online support center at www.biopac.com

    Audio ....................................................................................................................................................................... 223 

    Event Type Options ................................................................................................................................................. 223 

    Printing Events ......................................................................................................................................................... 228 

    Events and Waveform Editing ................................................................................................................................. 229 

    Constructing Graph Selections from Events ............................................................................................................ 229 

    Event Plotting and Variable Sampling Rate ............................................................................................................. 229 

    Chapter 12  Other Hardware Menu Commands ................................................................................................... 230 

    Show Input Values .................................................................................................................................................230 Manual Control (MP150 only) ...............................................................................................................................231 

    Set Up Linked Acquisitions ...................................................................................................................................233 

    Limitations on Linked Acquisitions synchronization methods ..............................................................................234 

    Manage Hardware Connections .............................................................................................................................235 

    MP150 Info ............................................................................................................................................................236 

    Segment Labels ......................................................................................................................................................236 

    Sound Feedback .....................................................................................................................................................236 

    Gauge .....................................................................................................................................................................238 Gauge Preferences.................................................................................................................................................... 238 

    Segment Timer “Stopwatch” option ........................................................................................................................ 240 

    Autoplot and Scroll ................................................................................................................................................242 

    Warn on Overwrite.................................................................................................................................................242 

    Organize Channel Presets ......................................................................................................................................243 

    PART C—ANALYSIS FUNCTIONS ................................................................................. 244 Toolbars ................................................................................................................................................................... 244 

    Shortcuts .................................................................................................................................................................. 244 

    Chapter 13  File Menu Commands .......................................................................................................................... 245 

     New .......... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ...........245 Graph Window ......................................................................................................................................................... 245 

     New > Graph-specific Journal ....... ........... .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ...... 245 

     New > Independent Journal ........... ........... .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ...... 245  New > Data View .......... .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .. 245 

     New > Batch Acquisition .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... 246 Batch Errors ............................................................................................................................................................. 246 

    Open .......................................................................................................................................................................248 

    Open Recent ...........................................................................................................................................................254 

    Open for Playback ..................................................................................................................................................254 

    SMI BeGaze Import ...............................................................................................................................................254 

    Dataquest Import ....................................................................................................................................................255 

    Close ......................................................................................................................................................................256 

    Save ........................................................................................................................................................................257 

    Save As ..................................................................................................................................................................257 

    Save Selection As...................................................................................................................................................263 

    Save Journal Text As .............................................................................................................................................264 

    File Format prompts ................................................................................................................................................. 264 Send Email Attachment .........................................................................................................................................265 

    Copy to Dropbox – Open from Dropbox ...............................................................................................................265  

    Page setup ..............................................................................................................................................................266 

    Print ........................................................................................................................................................................266 

    Quit ........................................................................................................................................................................267 

    Chapter 14  Edit Menu Commands ......................................................................................................................... 268 

    Undo / Can’t undo ..................................................................................................................................................269 

    Cut ..........................................................................................................................................................................269 

    Copy .......................................................................................................................................................................270 

    Paste .......................................................................................................................................................................270 

  • 8/17/2019 Acqknowledge 4 Software Guide

    7/565

    Table of Contents  7

    AcqKnowledge 4 Software Guide 

    Clear ....................................................................................................................................................................... 270 

    Clear all .................................................................................................................................................................. 270 

    Select All ............................................................................................................................................................... 271 

    Insert waveform ..................................................................................................................................................... 271 

    Duplicate waveform ............................................................................................................................................... 271 

    Remove waveform ................................................................................................................................................. 271 

    Remove last appended segment ............................................................................................................................. 271 

    Create Data Snapshot ............................................................................................................................................. 271 

    Merge Graphs ........................................................................................................................................................ 272 

    Merging graphs as data segments .......................................................................................................................... 273 

    Clipboard ............................................................................................................................................................... 273 

    Journal .................................................................................................................................................................... 275 

    Chapter 15  Transform Menu Commands .............................................................................................................. 278 

    Recently Used Transformations ............................................................................................................................. 279 

    Digital Filters ......................................................................................................................................................... 279 FIR Filters ................................................................................................................................................................ 282 

    Digital filter dialog ................................................................................................................................................... 282 IIR Filters ................................................................................................................................................................. 284 

    Adaptive Filtering .................................................................................................................................................... 285 

    Comb Band Stop Filter ............................................................................................................................................ 285 Fourier Linear Combiners ...................................................................................................................................... 288 

    Basic FLC ................................................................................................................................................................ 288 

    Weighted-Frequency FLC ....................................................................................................................................... 288 

    Math Functions ...................................................................................................................................................... 290 

    Template Functions ................................................................................................................................................ 292 

    Set Template ............................................................................................................................................................ 292 

    Remove mean .......................................................................................................................................................... 293 

    Template algorithms ................................................................................................................................................ 294 Adaptive Template Matching................................................................................................................................... 296 

    Integral ................................................................................................................................................................... 297 

    Derivative .............................................................................................................................................................. 298 

    Integrate ................................................................................................................................................................. 299 

    Output Reset ............................................................................................................................................................ 299 Smoothing .............................................................................................................................................................. 302 

    Difference .............................................................................................................................................................. 303 

    Resample ................................................................................................................................................................ 304 Resample Graph ....................................................................................................................................................... 304 

    Resample Waveform ................................................................................................................................................ 304 

    Expression .............................................................................................................................................................. 305 

    Delay ...................................................................................................................................................................... 305 

    Rescale ................................................................................................................................................................... 306 

    Waveform Math ..................................................................................................................................................... 306 

    Slew Rate Limiter .................................................................................................................................................. 307 

    Chapter 16  Analysis Menu Commands .................................................................................................................. 309 

    Histogram .............................................................................................................................................................. 309 

    Autoregressive Modeling .......... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... ......... 310 

     Nonlinear Modeling ........... .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ...... 311 

    Power Spectral Density .......................................................................................................................................... 313 

    AR Time-Frequency Analysis ............................................................................................................................... 313 

    FFT Fast Fourier Transformation ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ...... 314 

    Inverse FFT .............................................................................................................................................................. 317 

    DWT Discrete Wavelet Transformation ................................................................................................................ 319 

    Inverse DWT.......................................................................................................................................................... 319 

    Principal Component Analysis............................................................................................................................... 319 

    Inverse PCA ............................................................................................................................................................. 319 

  • 8/17/2019 Acqknowledge 4 Software Guide

    8/565

    8   AcqKnowledge 4 Software Guide 

    Visit the online support center at www.biopac.com

    Independent Component Analysis .........................................................................................................................320 

    Inverse ICA .............................................................................................................................................................. 320 

    Find Cycle (Peak Detector) ....................................................................................................................................321 

    Cycles/Peaks tab ...................................................................................................................................................... 321 

    Find Next Cycle .....................................................................................................................................................322 

    Find All Cycles in Graph .......................................................................................................................................322 

    Find in Selected Area .............................................................................................................................................322 

    Find All Cycles in Focus Areas .............................................................................................................................322 

    Find First Cycle ......................................................................................................................................................322 

    Preview (Selection tab) ..........................................................................................................................................322 

    Find Cycle definitions ............................................................................................................................................322 Selection tab ............................................................................................................................................................. 325 

    Output tab................................................................................................................................................................. 326 Output Measurements .............................................................................................................................................. 326 

    Output: Averaging—Offline .................................................................................................................................... 326 

    Output 3D Surface ................................................................................................................................................... 327 

    Output Events ........................................................................................................................................................... 328 

    Event definition ........................................................................................................................................................ 328 

    Event Location Table ............................................................................................................................................... 330 Output Focus Area ................................................................................................................................................... 331 

    Output: Clustering .................................................................................................................................................... 332 

    Algorithm Overview ................................................................................................................................................ 332 Clustering Settings ................................................................................................................................................... 333 

     Number of clusters .......... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... 333 Locate Cluster Centers ............................................................................................................................................. 333 

    Manually .................................................................................................................................................................. 333 

    By Learning ............................................................................................................................................................. 334 

    Training Set Definition ............................................................................................................................................ 334 

    Max. iterations ......................................................................................................................................................... 334 

    Tolerance.................................................................................................................................................................. 334 

    Remove Outliers ...................................................................................................................................................... 334 

    Clustering Criteria .................................................................................................................................................... 335 

    Clustering Output ..................................................................................................................................................... 335 

    Find Rate ................................................................................................................................................................338 

    Modes of Operation ...............................................................................................................................................338 Additional Find Rate Dialog Settings, Output Tab .......... .......... ........... .......... ........... .......... ........... .......... ........... .... 343 

    Specialized Analysis ..............................................................................................................................................344 

    Chapter 17  Specialized Analysis .......... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... . 345 

    Detect and Classify Heartbeats ..............................................................................................................................348 

    Locate Human ECG Complex Boundaries ............................................................................................................348 

    Locate Animal ECG Complex Boundaries ............................................................................................................349 

    Gastric Wave Analysis ...........................................................................................................................................349 

    Gastric Wave Coupling ..........................................................................................................................................349 

    Chaos Analysis .......................................................................................................................................................350 Detrended Fluctuation Analysis ............................................................................................................................... 350 

    Optimal Embedding Dimension ............................................................................................................................... 350 

    Optimal Time Delay................................................................................................................................................. 350 

    Plot Attractor ............................................................................................................................................................ 351 

    Correlation Coefficient ..........................................................................................................................................351 

    Electrodermal Activity ...........................................................................................................................................351 

    Derive Phasic EDA from Tonic ............................................................................................................................... 352 

    Event-related EDA Analysis .................................................................................................................................... 353 

    Sorting Options ........................................................................................................................................................ 355 

    Locate SCRs ............................................................................................................................................................. 358 

    EDA Measurements ................................................................................................................................................. 359 

    Electroencephalography .........................................................................................................................................361 Compute Approximate Entropy ............................................................................................................................... 361 

    Delta Power Analysis ............................................................................................................................................... 361 

  • 8/17/2019 Acqknowledge 4 Software Guide

    9/565

    Table of Contents  9

    AcqKnowledge 4 Software Guide 

    Derive Alpha RMS .................................................................................................................................................. 362 

    Derive EEG Frequency Bands ................................................................................................................................. 362 

    EEG Frequency Analysis ......................................................................................................................................... 363 

    Seizure Analysis ...................................................................................................................................................... 364 

    Seizure Analysis Parameters .................................................................................................................................... 365 

    Remove EOG Artifacts ............................................................................................................................................ 366 

    Preferences… ........................................................................................................................................................... 367 

    Electromyography .................................................................................................................................................. 368 

    Derive Average Rectified EMG ............................................................................................................................... 368 Derive Integrated EMG ............................................................................................................................................ 368 

    Derive Root Mean Square EMG .............................................................................................................................. 368 EMG Frequency and Power Analysis ...................................................................................................................... 369 

    Locate Muscle Activation ........................................................................................................................................ 369 

    Preferences… ........................................................................................................................................................... 370 

    Ensemble Average ................................................................................................................................................. 370 

    Epoch Analysis ...................................................................................................................................................... 372 

    Focus Areas between Events and Segments ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... . 373 

    Hemodynamic Analysis .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... 373 

    ABP Classifier ......................................................................................................................................................... 374 Arterial Blood Pressure ............................................................................................................................................ 374 

    ECG Interval Extraction .......................................................................................................................................... 375 

    Estimate Cardiac Output from ABP ......................................................................................................................... 376 Left Ventricular Blood Pressure .............................................................................................................................. 377 

    LVP Classifier.......................................................................................................................................................... 378 Monophasic Action Potential ................................................................................................................................... 379 

    MAP Classifier ........................................................................................................................................................ 380 

    Preferences ............................................................................................................................................................... 380 

    HRV and RSA Analysis......................................................................................................................................... 381 

    Multi-epoch HRV – Statistical ................................................................................................................................. 381 

    Multi-epoch HRV and RSA – Spectral .................................................................................................................... 382 

    R-R Poincare Plot .................................................................................................................................................... 383 

    Respiratory Sinus Arrhythmia (RSA Time-series) .................................................................................................. 384 Single-epoch HRV – Spectral .................................................................................................................................. 385 

    Frequency Bands .................................................................................................................................................... 387 

    PSD Options .......................................................................................................................................................... 387 Improvements to PSD Options (Acq Knowledge 4.3 and higher)........................................................................... 389 

    Impedance Cardiography Analysis ........................................................................................................................ 391 Body Surface Area ................................................................................................................................................... 391 

    dZ/dt Derive from Raw Z ........................................................................................................................................ 391 

    dZ/dt Classifier ........................................................................................................................................................ 391 

    ICG Analysis............................................................................................................................................................ 394 

    Ideal Body Weight ................................................................................................................................................... 397 

    PEP Pre-ejection Period ........................................................................................................................................... 397 

    dZ/dt Remove Motion Artifacts ............................................................................................................................... 398 

    VEPT ....................................................................................................................................................................... 398 Preferences ............................................................................................................................................................... 399 

    Magnetic Resonance Imaging ................................................................................................................................ 400 

    Artifact Frequency Removal .................................................................................................................................... 400 Artifact Projection Removal .................................................................................................................................... 402 

    Median Filter Artifact Removal ............................................................................................................................... 402 

    Signal Blanking........................................................................................................................................................ 403 

     Neurophysiology ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... ........... 404 

    Amplitude Histograms ............................................................................................................................................. 404 

    Average Action Potentials ....................................................................................................................................... 404 

    Classify Spikes ......................................................................................................................................................... 405 

    Dwell Time Histograms ........................................................................................................................................... 405 

    Find Overlapping Spike Episodes ............................................................................................................................ 406 

    Generate Spike Trains .............................................................................................................................................. 406 

    Locate Spike Episodes ............................................................................................................................................. 406 

    Set Episode Width and Offset .................................................................................................................................. 407 

  • 8/17/2019 Acqknowledge 4 Software Guide

    10/565

  • 8/17/2019 Acqknowledge 4 Software Guide

    11/565

    Table of Contents  11

    AcqKnowledge 4 Software Guide 

    Scaling Toolbar ........................................................................................................................................................ 433 

    Scope........................................................................................................................................................................ 433 

    Selection Palette ..................................................................................................................................................... 433 

    Spectrum Analyzer Palette ..................................................................................................................................... 434 

    Timers .................................................................................................................................................................... 434 

    Location Palette ..................................................................................................................................................... 434 

    Stacked Plot Options .............................................................................................................................................. 436 

    Step plot ................................................................................................................................................................. 436 

    Toolbar ................................................................................................................................................................... 436 

    X/Y ........................................................................................................................................................................ 436 Customize Toolbars ................................................................................................................................................. 437 

    Spectrum Analyzer Palette Details .......................................................................................................................... 438 

    Channel Information .............................................................................................................................................. 441 

    Preferences... .......................................................................................................................................................... 441 Measurements Preferences .......... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... ......... 445 

    Waveforms Preferences ........................................................................................................................................... 446 

    Event Summary Preferences .................................................................................................................................... 447 

    Graph Preferences .................................................................................................................................................... 447 

    Journal Preferences .................................................................................................................................................. 448 

    Hardware Preferences .............................................................................................................................................. 448 

    Performance Preferences ......................................................................................................................................... 448 

     Networking Preferences .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ........... .. 449 Other Preferences ..................................................................................................................................................... 449 

    Window Preferences ................................................................................................................................................ 450 

    Focus Areas Preferences .......................................................................................................................................... 450 

    Location Preferences ................................................................................................................................................ 450 

    Resetting All Preferences to Factory Default ........................................................................................................... 450 

    Scroll options ........................................................................................................................................................... 451 

    Size window... .......................................................................................................................................................... 451 

    Cursor Style ........................................................................................................................................................... 451 

    Create Data View ................................................................................................................................................... 451 

    Create Focus Area .................................................................................................................................................. 452 

    Organize Data Snaphots ......................................................................................................................................... 452 

    Show All Data Snaphots ........................................................................................................................................ 452 

    Load All Data Into Memory .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .......... ........... .... 452 

    Chapter 19  Program & OS Menus.......................................................................................................................... 453 

    Acq Knowledge menu ............................................................................................................................................. 453 

    Window menu ........................................................................................................................................................ 453 

    Bring All to Front .................................................................................................................................................... 453 

    Help menu .............................................................................................................................................................. 453 

    Chapter 20  Media Menu .......................................................................................................................................... 455 

    Synchronization Tip ........... .......... ........... .......... ........... ........... .......... ........... .......... ........... .......... ........... .......... ...... 456 

    Media > Set Up ...................................................................................................................................................... 457 

    Linked Media ......................................................................................................................................................... 458 

    Media > Capture .................................................................................................................................................... 458 Playback Preview ................................................................................................................................................... 458 

    Media Playback Example ...................................................................................................................................... 459 

    Media Capture Setup with CAM-HFR-A High Speed Camera .......... .......... ........... .......... ........... .......... ........... .... 461 

    PART D — LICENSED FUNCTIONALITIES ................................................................... 463 

    Chapter 21  Licensed Functionality: Network Data Transfer ............................................................................... 464 

    Data Connections ................................................................................................................................................... 465 

    Variable Sampling Rates ........................................................................................................................................ 465 

    Transfer Types ....................................................................................................................................................... 465 

  • 8/17/2019 Acqknowledge 4 Software Guide

    12/565

    12   AcqKnowledge 4 Software Guide 

    Visit the online support center at www.biopac.com

    Single Connection .................................................................................................................................................... 465 

    Multiple Connection ................................................................................................................................................ 466 

    XML-RPC ................................................................................................................................................................ 467 

    Transport Protocol..................................................................................................................................................467 

    TCP/IP ..................................................................................................................................................................... 467 UDP ......................................................................................................................................................................... 467 

    XML-RPC ................................................................................................................................................................ 468 

    Real-time Delivery Guarantees ..............................................................................................................................468 

    Data Formats ..........................................................................................................................................................469 Default Data Connection Settings ..........................................................................................................................469 

    Locating Acq Knowledge Servers ...........................................................................................................................470 

    Control Connections ..............................................................................................................................................470 

    TCP Port ................................................................................................................................................................470 

    Control Procedure Calls .........................................................................................................................................470 Channel Index Parameter Structures ........................................................................................................................ 470 

    Querying Acquisition Parameters ............................................................................................................................ 471 

    Data Connection Configuration Commands ............................................................................................................ 472 

    Reading Data During Acquisition ............................................................................................................................ 475 

    Other Control Connection Commands ..................................................................................................................... 476 

    Chapter 22  Licensed Functionality: Vibromyography .......................................................................................... 478 

    Sampling Rate Restrictions ...................................................................................................................................... 478 Transducer Setup...................................................................................................................................................... 478 

    Post-Analysis Selection Adjustment ........................................................................................................................ 479 

    Data Modification History Name ............................................................................................................................. 479 VMG Calculation Channel Preset ............................................................................................................................ 479 

    VMG Sample Data Files .......................................................................................................................................... 480 

    Chapter 23  Licensed Functionality: Scripting .............................................................................