ac dermal respiratory factor advanced pf - active concepts

AC Dermal Respiratory Factor Advanced PF

Anti-Aging Technology Using Saccharomyces cerevisiae

Increases Cell Respiration + Collagen & Elastin Synthesis + Enhances Cell Metabolism


Technical Information:

Product Code: 20219PF

INCI Name: Water + Saccharomyces Lysate Extract

INCI Status: Conforms

Suggested Use Levels: 0.5 – 1.0%

Suggested Applications: Increases Cellular Respiration + Enhances Cellular Metabolism + Collagen/ Elastin Synthesis + Anti-Aging

Solubility: Water Soluble

AC DERMAL RESPIRATORY FACTOR ADVANCED PF

Saccharomyces cerevisiae

• Saccharomyces cerevisiae live yeast cells are struck with specific wave lengths of UV radiation

Prompts live yeast cells to secrete active compound

Biofermenation and various filtration techniques are used to then isolate and extract the live yeast secretion

• Non-Animal Derived + Non-Dairy

• Secretion produced from the most well-known yeast Baker’s Yeast/ Brewer’s Yeast

AC DERMAL RESPIRATORY FACTOR ADVANCED PF

Product Benefits:

Increases cellular respiration

Increases cellular proliferation

Enhances cellular metabolism

Promotes collagen + elastin synthesis

Extraordinary skin soothing properties

Ideal for use in baby care, after sun care, sensitive skin, scalp care applications and to provide potent anti-aging benefits

Live Yeast Cell Derivatives (LYCD)

LYCD

• Commercial use began in the American Home Product’s market

LYCD was the main active in an anti-haemorrhoid cream – Prep H

• Adventurous fashion models can be thanked for the crossover of LYCD into cosmetic applications for soothing

• Sleep deprivation caused bags under the eyes• Models used this LYCD containing cream to reduce the

appearance of bags and swelling

In North America + South America + Europe LYCD is primarily a cosmetic potentiater: Stimulates Oxygen Consumption

Combats Irritation

Reduces Irritation: Mechanism of Action

Live Yeast Cell Derivatives

• LYCD’s role in irritation reduction became clearer with a more focused study of Heat Shock Proteins (HSP)

• When cells are subjected to stress, proteins are produced to:

Duplicate the functions of disabled cell Protect the cell against additional stress

• HSPs are a component of LYCD – these specific Heat Shock Proteins are similar to human cells & can be applied to help reduce or reverse the actual stress in human cells

Potentiation of Activity

Live Yeast Cell Derivatives

• LYCD is a global metabolic stimulant

• Capable of potentiating the activity of other materials in a cosmetic/ personal care formulation

• LYCD utility is evidenced in Avon’s patents US 5,676,956 and US 5,643,587where it’s used to increase the activity of Vitamin C derivatives

Yeast Species

Saccharomyces cerevisiae

• Fermentation abilities

• Very similar to human cells in composition and structure• True eukaryote• Only 17 chromosomes (humans have 23) but

structurally similar• Two genders, reproduces with cells (similar to

human cell fertilization)

• First organism to have a completely mapped genome

• Egyptians are thought to have discovered the beneficial properties of yeast:• Over 6,000 years ago• Leavened breads, wine and beer

Yeast + Fermentation

Discovering Fermentation

• Said that yeast cells found their way to an open barrel of crushed grapes and the barrel contents turned into wine! Residual yeast sediment was then added to

subsequent grape barrels Wine production process born!

• In the 1850’s, Louis Pasteur discovered that fermentation was simply the metabolic by-product of yeast

• In 1896, German chemist Eduard Buchner discovered that fermentation enzymes in yeast remained active even after cell extraction – this paved the way for the delineation of biochemical process of fermentation

Yeast + Biofermentation

Dr. George Sperti

• 1930’s – Performed research using UV radiation and yeast cells

• Live yeast cells were injured with UV radiation and secretions from the cells were collected

• His findings and methods of LYCD production were immortalized in three patents!

• Discoveries after secretion extraction was added to normal cells:

Significant Increase in Cellular Respiration + Increase in Cellular Replication + Enhanced Glycolysis + Enhanced Cellular Metabolism

Isolated LYCD were shown to stimulate cells from other eukaryotes

In-vivo Sensorial Soothing AssayProtocol

• Evaluate the ability of AC Dermal Respiratory Factor Advanced PF to reduce the discomfort produced by overexposure to UV radiation

• 20 panelist (m/f) evaluated two lotions containing either AC DRF Advanced PF (2.8% w/w) or Benzocaine (0.5% w/w)

• Panelists applied lotions immediately after sun exposure

• Recorded initial soothing perception and perception after 2 hours (1 to 5 with 5 being complete reduction in pain)

Topical application of AC Dermal Respiratory Factor Advanced PF is capable of producing consumer perceivable reduction in both erythema and pain

caused due to overexposure to UV light.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

0.1 0.2 0.15

1.45

1.151.3

3.6

3.95

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

t = 0 Hr t = 2 Hr t = 0 Hr t = 2 Hr t = 0 Hr t = 2 Hr t = 0 t = 2 Hr

Benzocain AC DRF Advanced PF Benzocaine AC DRF Advanced PF

Reduction of Erythema Reduction of Sunburn Pain

Per

ceiv

ed

Red

uct

ion


0

30

60

90

120

150

180

50mg/mL IGF-1 .01% AC DRF Advanced .1% AC DRF Advanced 1.0% AC DRF Advanced

Per

cen

t (%

) In

crea

se

Protocol

• Collagen is a major component of the dermis & provides structure & elasticity to the skin

• Three dose levels were compared to the control – .01%, .1% & 1%

• Results were determined by the ELISA Assay

• According to the results, all concentrations of AC Dermal Respiratory Factor Advanced PF was effective at increasing collagen synthesis

In-vitro Collagen Production Assay

Figure 2. Increase in collagen production following application of AC Dermal Respiratory Factor Advanced PF .


Procollagen Assay

Protocol

• An ELISA assay was used to assess AC DRF Advanced impact on collagen synthesis

• The results indicate that AC DRFAdvanced PF is capable ofincreasing the expression of type1-C Peptide in a fibroblast cellculture model

• Useful in cosmetic formulations intended to increase collagen synthesis

Figure 3. Comparison of the effects on procollagen levels following treatment.

0

500

1000

1500

2000

2500

3000

3500

1% Magnesium

Ascorbyl Phosphate

0.5% Magnesium

Ascorbyl Phosphate

1% AC DRF Advanced

PF

0.5% AC DRF

Advanced PF

4mM Sodium Butyrate Untreated

Type

1 C

-Pep

tide

(ng/

ml)


ORAC Assay

97

13

116

46

15

0

20

40

60

80

100

120

140

100µM Trolox 12.5µM Trolox 2% 20219 AC DRFAdvanced

1% 20219 AC DRFAdvanced

0.5% 20219 ACDRF Advanced

An

tio

xid

ant

Cap

acit

y (µ

MTE

)

Protocol

• Trolox is used as the positive control

• Solutions were prepared at two concentrations for a reference

• Fluorescent measurements were taken every 2 minutes for 2 hours

• AC Dermal Respiratory Factor Advanced PF showed antioxidant activity at all concentrations tested.

Figure 4. Results of antioxidant capacity of test materials.


Pigmentation Assay

Protocol

• Equipment: DermaLab Skin Combo

• 10 volunteers M/F between the ages of 23 and 45

• Measure the erythema levels of the subject’s volar forearms

• Baseline pigmentation readings were taken on day one of the study

• AC Dermal Respiratory Factor Advanced PF is capable of decreasing erythema when compared to both the untreated control as well as the base lotion

Figure 5. Pigmentation Results for Individual Test Sites.

10.00

10.50

11.00

11.50

12.00

12.50

13.00

13.50

T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks

ErythemaAverage Results for Individual Test Sites

Experimental (2.0% AC DRF Advanced PF

+ Base Lotion)

Base Lotion

Untreated


Pigmentation Assay

Protocol



• Measure the erythema levels of the subject’s volar forearms

• Baseline pigmentation readings were taken on day one of the study

• AC Dermal Respiratory Factor Advance PF showed reductions in erythema at a 2.0% concentration

Figure 6. Pigmentation Results Compared in Percent Difference between Test Sites.

-20.00%

-15.00%

-10.00%

-5.00%

0.00%

5.00%

10.00%


Perc

ent

(%)

Diff

eren

ce

ErythemaComparative Data Between Two Sites

Base Lotion vs. Untreated

Experimental (2.0% AC DRF

Advanced PF + Base Lotion) vs.

Untreated


Advanced PF + Base Lotion) vs. Base

Lotion


High Resolution Ultrasound Skin Imaging Assay

Protocol



• Determine the skin density of the subject’s volar forearms

• Apply 2 mg of each test material on their volar forearms

• Concentration: 2%

• AC Dermal Respiratory Factor Advanced PF is capable of improving skin density when compared to both the untreated control as well as the base lotion

Figure 7. Ultrasound Results Comparing Test Sites to Untreated Site.

-10.00%

-5.00%

0.00%

5.00%

10.00%

15.00%

20.00%

25.00%

T = 0 T = 1 Week T = 2 WeeksT = 3 WeeksT = 4 Weeks

Perc

ent

(%)

Diff

eren

ce

Collagen UltrasoundComparison of Experimental + Control to Untreated Site




Untreated


Hyaluronic Synthesis Assay

Protocol

• In-vitro Hyaluronic Acid Synthesis was performed using human keratinocytes

• Cultured in KSFM medium and incubated for a period of 24 hours at a temperature of 37C with the concentration of carbon dioxide in the incubator being limited to 5%

• Concentrations: 0.5%, 1.0%

• Incubated under the same conditions as before for a period of 72 hours

• AC Dermal Respiratory Factor Advanced PF is capable of increasing the percent concentration of hyaluronic acid by approximately 42% in comparison to the control

Figure 8. Hyaluronic Acid Production comparing AC Dermal Respiratory Factor Advanced PF and Control.

0

20

40

60

80

100

120

140

160

Control 0.5% AC Dermal RespiratoryFactor Advanced PF

1.0% AC Dermal RespiratoryFactor Advanced PF

Per

cen

t (%

) In

crea

se


Cellular Viability Assay Analysis

Protocol• Human dermal fibroblasts were seeded into

96-well tissue culture plates

• Concentrations: 0.1%, 0.01%

• AC Dermal Respiratory Factor Advanced PF, nor the preservatives exhibited any inhibition of cell viability

• It can therefore be concluded that at normal use concentrations AC Dermal Respiratory Factor Advanced PF is not cytotoxic

-15%

-10%

-5%

0%

5%

10%

15%

0

20000

40000

60000

80000

100000

120000

Complete Media 0.1% AC DermalRespiratory Factor

Advanced PF

0.01% AC DermalRespiratory Factor

Advanced PF

MFU

MFU

% Change

Figure 9. Cellular Metabolism of AC Dermal Respiratory Factor Advanced PF-

treated fibroblasts expressed in terms of percent of control.


Moisturization AssayProtocol• Equipment: DermaLab Skin Combo


• Apply on volar forearms

• Moisture levels were improved by 44.60% after 24 hours and by 95.0% after 4 weeks when compared to the untreated control

• When compared to the base cream, AC DRF Advanced PF improved moisturization by 33.74% after 4 weeks

0.00

50.00

100.00

150.00

200.00

250.00

T = 0 T = 24Hours

T = 1Week

T = 2Weeks

T = 3Weeks

T = 4Weeks

T = -24Hours

T = -1Week

Mo

istu

riza

tio

n (μ

Sie

me

ns)

Average Moisture Readings

Experimental (2.0% ACDRF Advanced PF +Base Lotion)

Base Lotion

Untreated

Figure 10. Average increase in moisturization per test site.


Moisturization Assay

0.00

10.00

20.00

30.00

40.00

50.00

60.00

70.00

80.00

90.00

100.00

T = 0 T = 24Hours

T = 1Week

T = 2Weeks

T = 3Weeks

T = 4Weeks

T = -24Hours

T = -1Week

Pe

rce

nt

(%)

Dif

fere

nce

Be

twe

en

Te

st S

ite

s

Percent (%) Difference


Experimental (2.0% ACDRF Advanced PF + BaseLotion) vs. Untreated

Experimental (2.0% ACDRF Advanced PF + BaseLotion) vs. Base Lotion

Figure 11. Percent difference in moisturization between two test sites over four weeks.

Protocol• Equipment: DermaLab Skin Combo



• 2.0% AC DRF Advanced PF + Base Lotion moisturized the skin 19.76% better after 24 hours and was still 9.01% more effective in moisturizing the skin when reading were taken one week after the applications of both test materials ceased


Moisturization Assay

Figure 12. Regression in skin moisturization after application of experimental material ceased.

Protocol• Equipment: DermaLab Skin Combo



• AC Dermal Respiratory Factor Advanced PF is not only capable of providing functional benefits but it is also capable of providing moisturizing and skin hydrating benefits when added to cosmetic applications

0.00

10.00

20.00

30.00

40.00

50.00

60.00

70.00

80.00

90.00

100.00

T = 0 T = 24 Hours T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks T = -24 Hours T = -1 Week

Perc

ent

(%)

Diff

eren

ce

Moisture RegressionExperimental Treatment vs. Untreated


TEWL AssayProtocol• Equipment: DermaLab Skin Combo


• Measure TEWL on the subject’s volar forearms; apply 2 mg of each test material

• AC Dermal Respiratory Factor Advanced PF showed very effective moisture retention capabilities at a 2.0% concentration

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00


Op

en

Ch

amb

er

Wat

er

Leve

ls

Average Moisture Loss Readings

Experimental (2.0%AC DRF AdvancedPF + Base Lotion)

Base Lotion

Untreated

Figure 13. TEWL measurements taken at individual test sites


TEWL AssayProtocol• Equipment: DermaLab Skin Combo


• Measure TEWL on the subject’s volar forearms; apply 2 mg of each test material

• When compared to the base cream, AC Dermal Respiratory Factor Advanced PF was shown to decrease transepidermal water loss by 29.24% and by 50.00% when compared to the untreated control after 4 weeks

• Promotes moisture retention benefits when added to cosmetic applications

-60.00%

-50.00%

-40.00%

-30.00%

-20.00%

-10.00%

0.00%Base Lotion vs. Untreated Experimental (2.0% AC DRF


Untreated


Advanced PF + Base Lotion) vs. Base

Lotion

Perc

ent

(%)

Diff

eren

ce

Transepidermal Water LossComparison of Test Sites - Water Loss Reduction Over Time

T = 0

T = 4 Weeks

Figure 14. Comparison of percent reduction in water loss over time between two test sites.


In-vitro Scratch AssayProtocol• Human dermal fibroblasts were

seeded into 6-well tissue culture plates

• 5% concentration of AC Dermal Respiratory Factor Advanced PF was added to the serum-free DMEM and incubated with fibroblasts

• Scratch took place every 24 hours and up to 96 hours

• AC Dermal Respiratory Factor Advanced PF was able to increase cell migration and close the scratch at a rate comparable to the positive control

• Product has healing abilities and cell proliferation properties

0

0.5

1

1.5

2

2.5

3

3.5

0 24 48 72 96

Scra

tch

Are

a (m

m2)

Time Pose Scratch (Hours)

Area Enclosure

Positive Control Negative Control AC Dermal Respiratory Factor Advanced PF

0

20000

40000

60000

80000

100000

120000

24 48 72 96

Mig

rati

on

Rat

e (n

m2/

ho

ur)

Time Post Scratch (Hours)

Migration Rate

Positive Control Negative Control AC Dermal Respiratory Factor Advanced PF


Additional Data• Dermal and Ocular Irritation Tests: Non-Irritating


Technical Information:

Product Code: 20219PF

INCI Name: Water + Saccharomyces Lysate Extract

INCI Status: Conforms

Suggested Use Levels: 0.5 – 1.0%

Suggested Applications: Increases Cellular Respiration + Enhances Cellular Metabolism + Collagen/ Elastin Synthesis + Anti-Aging

Solubility: Water Soluble

THANK YOU

For more information –Visit our website!www.activeconceptsllc.com


http://activeconceptsllc.com/

ac dermal respiratory factor advanced pf - active concepts

Documents