a s y · clear path to scale up all infors ht bioreactors are based on equivalent technical...
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Benchtop Bioreactor
MiniforsUnpack, Install and Ferment
May be subject to technical amendments
Designed for
easy handlin
g
Simple Operat
ion
Complete Pack
ages
Exellent Value
Builds Bridges
www.infors-ht.com
++ HANDLING
++
++
EASY ++
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Maximum capability for minimum price
The basic system delivers practically all the usual process parameters for fermentation.• Stirrer speed• Temperature• pH• pO2• Antifoam/level• Feed• PC Software: Iris
With the optional 2 addi tional analogue, inputs/outputs, the system can cater for additional parameters if required, such as, Mass-Flow, turbidity, exit gas analysis and additional external analogue pumps.
Designed for Easy Handling Minifors is designed to make handling as easy as possible for new users
with „plug’n’pump”, the use of a heating block rather than a jacket and an integral frame for carrying all components to the autoclave together.
Simple Operation Setting up the Minifors is made as easy as possible with a control panel that
is very similar to that used on our Multitron incubator shakers.
Complete Packages Minifors comes as a package with Cell and Bacterial versions plus two ves-
sel sizes. It can be set up „out of the box” with the necessary accessories provided.
Excellent Value The price/performance ratio of Minifors is high with a full system being
comparable in cost to a large incubator shaker but with the ability to provi-de more culture in less space.
Builds Bridges Minifors provides the link between shake flasks and larger-scale fermenta-
tion by providing the shake flask user with the additional features they need most i.e. volume, sampling, pH measurement/control, oxygen control, the ability to add feed and PC data-logging/control.
Benchtop Bioreactor | Minifors
Complete Specifications Additional parameters
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Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
Infors GmbH
Dachauer Str. 6D-85254 EinsbachT +49 (0)8135 83 00F +49 (0)8135/83 [email protected]
Infors UK Ltd
The Courtyard Business Centre, Dovers Farm Lonesome Lane, ReigateSurrey, RH2 7QT, VK T +44 (0)1737 22 31 00F +44 (0)1737 24 72 [email protected]
Infors Benelux BV
Fabriekstraat 387005 AR DoetinchemP.O. Box 613NL-7000 AP DoetinchemT + 31(0)314-36 44 50F + 31(0)314-37 80 [email protected]
Infors Canada
8350 rue BombardierAnjou, QuebecCanada H1J 1A6T + 1 514 352 50 95F + 1 514 352 56 [email protected]
Infors Bio-Technology (Beijing) Co., Ltd.Room 505C, Building 106Lize ZhongyuanWangjing New Industrial ZoneChaoyang District, Peking100102 Volksrepublik ChinaT +86 10 51652068F +86 10 [email protected]
For more information please visit: www.infors-ht.com
Infors Sarl
2 rue du Buissonsaux fraisesBâtiment D13FR-91300 Massy T +33 (0)169 30 95 04F +33 (0)169 30 95 [email protected]
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++ R ES ULTS
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RAPID ++
Multifors 2Parallel bioreactor for rapid and statistically accurate process data
Benchtop Bioreactor
May be subject to technical amendments www.infors-ht.com
Faster results w
ith optimised par
allel handling
Flexible and a
pplication-optim
ised configurati
ons
Space-saving,
compact design
OPC server for
unlimited conn
ection
Precise control
of your bioproc
ess
100% aseptic
sampling
Clear path to s
cale up
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Small scale parallel bioreactor
Benchtop Bioreactor | Multifors 2
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Faster results with optimised parallel handling Multifors has been designed to make user handling as easy as possible. A sup
port frame carries both culture vessels, 4 reagent bottles, 8 pump heads and 2 sample bottles all together for autoclaving (see images on the right).
Flexible and application- optimised configurations With a choice of preconfigured packages and an extensive list of options,
Multifors 2 is always application optimized for your microbial or cell culture bioprocess.
Space-saving compact design An extremely compact freestanding device, with two completely individual
bioreactors located within a single base unit. Up to 6 bioreactors can be connected to one controller and require approx. 1.2 meter of bench space.
OPC server for unlimited connection Connecting external devices such as HPLC, MS, glucose analyser, etc., is pos
sible via analog in/out signals, Modbus or OPC XML DA. The integrated OPC server also provides a link to SCADA software via a network.
Precise control of your bioprocess Bioprocess control begins with the quality and flexibility of the local control
ler. The Multifors 2 touch screen controller provides accurate and reliable measurement, control and communication with supervisory software.
100% aseptic sampling The INFORS HT Super Safe Sampler allows you to take smallest samples with
out wasting any culture. The truly aseptic design makes contamination during sampling impossible.
Clear path to scale up All INFORS HT bioreactors are based on equivalent technical features and
control systems. This guarantees uniform and consistent handling from process development to pilot scale. What is your next step?
Dimensions (WxDxH): 350 x 500 x ~850 mm Working volumes: 180–500 mL / 320–1000 mL (bacterial system); 150–500 mL / 220–750 mL (cell culture), in pairsMaximum expansion: 3 base units per touch screen controller, i.e. 6 vesselsSpeed ranges: 100–1200 rpm for bacterial systems, 30–300 rpm for cell culturesTemperature range: ~5°C above coolant to 60°C Standard parameters: stirrer speed, temperature, pH, pO2, antifoam/level, feed, gas mix, gas flow
Key technical data
• Process development• Process optimisation• Scale up• Scale down• Statistical studies• Process analytical technology (PAT)• Media development• Growth studies• Toxicity testing• High throughput screening
Applications
4 easy steps and you’re ready to go
1) Replace vessel 2) Fit plates with holder on base unit pump heads
3) Prime pumps 4) Synchronised
automatically start of all in groups bioreactors
For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
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May be subject to technical amendmentswww.infors-ht.com
FOR FLEXIBIL
ITY
+D
ESIGNED+
Benchtop Bioreactor
Labfors 5Universal applicability for single or parallel bioprocesses with no compromises
Flexible and a
pplication-optim
ised
configurations
Splashproof to
uch screen with
OPC server
Parallel biorea
ctor option
Faster results w
ith optimised han
dling
100% aseptic
operation and sa
mpling
Space-saving,
compact design
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Flexible and application-optimised configurations
The Labfors 5 bench-top bioreactor provides an easy-to-handle, flexible and upgradable system for culture volumes from 0.5 L up to 10 L. Pre-configured packages for cell cultures and microbials are available. Special options even qualify the Labfors 5 for cultivation of phototrophic organ-isms, halophile microorganisms and for biofuel applications. A single-use vessel option adds further flexibility.
Splashproof touch screen with integrated OPC server
Bioprocess control begins with the quality and flexibility of the local control-ler. The Labfors 5 splashproof touch screen controller provides accurate and reliable measurement and control for up to 6 vessels. Connecting external devices such as balances, HPLC, MS, Glucose analyser, SCADA software, etc., is easily possible via OPC.
Parallel bioreactor option The Labfors 5 features a new parallel bioreactor option which allows up to
6 vessels to be independently controlled from a single touch screen. Special features optimise the system for parallel bioreactor operation.
Faster results with optimised handling
The Labfors 5 high-precision pump heads can be removed on a single plate in groups of 4 and autoclaved with the vessel. This reduces handling time and handling errors considerably as tubing does not need to be threaded around the pump heads and adjusted before use.
100% aseptic sampling The INFORS HT Super Safe Sampler allows you to take smallest samples
without wasting any culture. The truly aseptic design makes contamination during sampling impossible.
Space-saving, compact design With a footprint as low as 465 x 465 mm, the Labfors 5 saves valuable
bench space. This reduces the overall cost and allows greater research ca-pacity in the same space.
Benchtop Bioreactor | Labfors 5
Universal applicability
• High-density cultures for protein production
• Photosensitive cultivations
• Biofuels• Process Analytical
Technology (PAT)• Process development
• Process optimization• Scale up• Scale down• Statistical studies• Growth studies• Anaerobic cultures• Halophile strains• Brewing research
Applications
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Minimum dimensions: 465 x 465 x 900 mm (W x D x H)Vessels: 2; 3.6; 7.5; 10; 13 L total volumeMaximum expansion: Up to 6 base units per touch screen controllerSpeed range: 10–1500 rpm direct drive, 20– 300 rpm magnetic drive (depending on vessel and motor options)Temperature range: ~5°C above coolant to 60°C (water jacket) or 95°C (heating pad) Pump flow rate: 0.03– 3.3 mL/min (standard tubing),0.2–17.9 mL/min (large-size tubing), 0.001–1.4 mL/min (small-size tubing)Standard parameters: Stirrer speed, temperature, pH, pO2, antifoam/level, feed, gas mix, gas flow
Key technical data
For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
Parallel bioreactor option
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May be subject to technical amendmentswww.infors-ht.com
LED Flat Panel OptionPerfect for biofuel research
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++ E N E R G
Y+
+
++
GREEN ++
High-power LE
D lighting
Ideal light dist
ribution
Airlift
Scale up
Controllable lig
ht intensity
Choice of light
spectra
Multiple appli
cations
Labfors 5 Lux | Option
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2May be subject to technical amendments YP 792005eMay be subject to technical amendments YP 792005e
For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
High-power LED lighting A total of 260 water-cooled high-power LEDs, focused with reflectors, offer
a reproducible light source with a long service life. The maximal irradiation intensity of approx. 3000 µmol/m²s is comparable to Caribbean sunlight. The energy efficiency is approx. five times greater than for fluorescent tubes.
Ideal light distribution With a layer thickness of only 2 cm, the flat panel culture vessel offers a uni-
form irradiation strength for the entire culture without shadowing effects.
Airlift The mixing of the culture is achieved gently yet effectively using the Airlift
process. Foam formation and biofouling are both reduced thanks to the asymmetrical shape of the head space.
Scale up A scaling up of the flat panel culture vessel to a pilot or production scale is
possible.
Controllable light intensity Continuously variable electronic dimming from 0–100% facilitates the pre-
cise adjustment of light intensity. When used in conjunction with Iris parallel bioprocess control software, not only are on/off cycles possible, but even the simulation of a daylight curve.
Choice of light spectra Warm white light in the standard design offers an ideal spectrum in the
visible range that is similar to sunlight and contains a high proportion of photosynthetically-active light. Alternatively, other light colours such as red, blue, UV-A or infrared can be selected. The dimming of three colours inde-pendently of one another, allowing a dynamic change to the spectrum to be achieved, is optional.
Multiple applications Designed for the cultivation of algae, plant cells and cyanobacteria, the
Labfors 5 Lux is ideal for every photosynthetic process, e.g. investigations into the feasibility of biofuel production using algae. The Labfors 5 Lux control unit is also perfectly suited for other applications, e.g. for bacteria or mammalian cell cultures – depending on the specification.
Labfors 5 Lux | Option
High standard – high productivity
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Total volume: 1.9 LWorking volume: 1.6–1.8 L Light: approx. 3000 µmol/m2s(approx. 180,000 lux)
Standard parameters:• Temperature• light• pH• pO2• antifoam/level• feed• Gas mix• Gas flow
Additional parameters: Easy integration of many online systems, e.g. extra sensors, balances, external pumps• 4 x Analogue In• 6 x Analogue Out• 2 x Digital OutAdvanced process control features via Iris software, e.g. regulated control of medium feed
Key technical data
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May be subject to technical amendmentswww.infors-ht.com
LED Stirred Tank OptionThe traditional Labfors 5 Lux stirred tank for photosynthesis applications
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+++ V E R S I O
N+
++
++
+NEW+++
New: LED light
ing
Extremely adap
table
Traditional sti
rred tank vessel
Controllable lig
ht intensity
Choice of light
spectra
Multiple appli
cations
Labfors 5 Lux | Option
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2May be subject to technical amendments YP 792005eMay be subject to technical amendments YP 792005e
For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
New: LED lighting A total of 16 LED strips with air cooling offer a reproducible light source with
a long service life. The maximum light intensity of approx. 700 µmol/m²s is entirely sufficient for the majority of applications. The energy efficiency is approx. five times greater than for fluorescent tubes.
Extremely adaptable The LED strips are replaceable individually, which in turn also allows the
light spectrum to be matched to the application at a later point.
Traditional stirred tank vessel The standard cultivation technique on a laboratory scale.
Controllable light intensity Continuously variable electronic dimming from 0–100% facilitates the pre-
cise adjustment of light intensity. When used in conjunction with Iris parallel bioprocess control software, not only are on/off cycles possible, but even the simulation of a daylight curve.
Choice of light spectra Warm white light in the standard design offers an ideal spectrum in the
visible range that is similar to sunlight and contains a high proportion of photosynthetically-active light. Alternatively, other light colours such as red, blue, UV-A or infrared can be selected. The dimming of three colours inde-pendently of one another, allowing a dynamic change to the spectrum to be achieved, is optional.
Multiple applications Designed for the cultivation of algae, plant cells and cyanobacteria, the
Labfors 5 Lux is ideal for every photosynthetic process, e.g. investigations into the feasibility of biofuel production using algae. The Labfors 5 Lux control unit is also perfectly suited for other applications, e.g. for bacteria or mammalian cell cultures – depending on the specification.
Labfors 5 Lux | Option
Now high tech with LED!
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Total volume: 3.6 LWorking volume: 0.6–2.4 L Light: 600–700 µmol/m2s (approx. 50,000 lux)
Standard parameters:• Stirrer speed • temperature• light• pH• pO2• antifoam/level• feed• Gas mix• Gas flow
Additional parameters: Easy integration of many online systems, e.g. extra sensors, balances, external pumps• 4 x Analogue In• 6 x Analogue Out• 2 x Digital OutAdvanced process control features via Iris software, e.g. regulated control of medium feed
Key technical data
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LabCIP Automatic cleaning and sterilisation at the push of a button
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Labfors 5 Accessories | LabCIP
Automatic clea
ning (CIP)
Automatic ster
ilisation (SIP)
High through
put
Easiest operati
on
Robust, prove
n technology
Small footprin
t
Cost-saving
+TH RO U GH
PUT
+
++
D
OUBLE++
« The Labfors 5 with LabCIP allowed us to double the throughput from 8 to 16 protein expressions per week. As a result, we make a greater contribution to the success of drug discovery projects.» Dr. Alvar Gossert, Investigator II, Novartis AG, Basel, Switzerland
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Just science! No cleaning.
Labfors 5 Accessories | LabCIP
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For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
Automatic cleaning (CIP) The reliable and reproducible base and/or acid cleaning is done automat-
ically with variable cycles. This cleaning method is used as a standard in GMP cleaning processes, and can be validated.
Automatic sterilisation (SIP) Sterilisation of the Labfors 5, e.g. with hot sodium hydroxide solution, and
the final rinse with sterile water (integrated sterile filter) proceeds automat-ically. All parts in contact with the product including the air feed and ex-haust system of the bioreactor are included in the rinse. The process condi-tions (temperature, time, pH) can be configured flexibly. Typical sterilisation conditions are 60°C, pH 12.8, for a duration of 2 h.
High throughput The CIP/SIP saves you an enormous amount of time. The Labfors 5 can be
cleaned and sterilised automatically, e.g. overnight, and is then immediately ready for use on the next morning. This gives you more time for science and allows you to increase the throughput up to two-fold.
Easiest operation There is no need to dismantle and reassemble the bioreactor for cleaning
purposes or to transport the vessel to the autoclave and back. This elim-inates the sources of error and handling risks inherent in these steps.
Robust, proven technology There is no single-use technology applied in the bioreactor. Its sustainability
and robustness are arguments in favour of the Labfors 5 with LabCIP. The precise sensors and the powerful stirrer of the Labfors 5 ensure high cell densities and protein titers.
Small footprint The LabCIP needs less bench space than an A4-sized sheet of paper and can
be placed behind the Labfors 5.
Cost-saving The unit saves valuable working time, since it takes less than 5 minutes to
get the bioreactor ready for use. The downtime of the Labfors 5 is thus min-imised. There are hardly any running expenses associated with preparation.
Dimensions (W x D x H): 210 x 295 x 1000 mm Labfors 5 compatibility: All Labfors 5 bioreactors (microbial configuration) are compatible with the LabCIP: 2; 3,6; 7,5; 13 l total volume
Key technical data
• High throughput bioprocesses
• Fermentation• High throughput
protein expression, e.g. antibodies
• Microbial bioprocesses (E. coli, P. pastoris etc.)
• Process development / process optimisation
• Scale down / Scale up
Applications
Possible flow diagram of an automatic cleaning and sterilisation processThe parameters, pH, temperature, time, number of cycles, can be configured individually. The individual phases can be repeated as desired.
2x CIP (base): e.g. NaOH, 60°C, 30 min
Neutralisation, discard and rinsing
CIP (acid): e.g. H3PO4, 40°C, 30 min
Neutralisation, discard and rinsing
Sterilisation in place: e.g. NaOH, 60°C, 2 h
Neutralisation, discard
Sterile rinsing: deion. H2O via integrated sterile filter
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+ USER FRIEND
LY +
CO
MPACT &
All around acc
ess
Compact
Easy navigatio
n
Simple yet Flex
ible
Low volume Sp
ecialist
Pilot Bioreactor
Techfors-SThe solid basis for your Pilot Fermentation
May be subject to technical amendments www.infors-ht.com
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High-Tech for an entry-level price
Pilot Bioreactor | Techfors-S
All around access Techfors-S has the inlet and exit filters mounted behind the top plate surface
for easy access to all parts. Being mobile, Techfors-S can even be swivelled for rapid access to the open-frame components at the rear.
Compact The single column design of Techfors-S makes the unit compact and capa-
ble of fitting into almost any laboratory. A steam generator can be integra-ted into the same footprint, if required.
Easy navigation The Touchscreen ontroller uses tabs and a colour touch screen display for easy, intuitive navigation. Both new users and advanced operators are catered for.
* The X-DDC operating panel with navigation knob is still available as an option on Techfors and Techfors-S – see separate brochure for details.
Simple yet Flexible Techfors-S was designed to bring in-situ sterilizable systems to people new
to this class of fermenter and it achieves this while allowing for expansion, re-configuration and open-frame gas mixing.
Low volume Specialist The jacket of each Techfors-S vessel extends below the sides of the vessel,
providing good temperature control even when low volumes are used – vi-tal for modern fed-batch applications.
Bacterial and Cell Version:• Vessel size 7,5 L ; 15 L or 30 L TV• Sterilisation In Situ• Temperature Ambiant/coolant +5 – 70°C• Vessel types 316L; Double Jacket• Speed
Bacterial Version 50–1200 RPM Cell Version 20–300 RPM
• Vessel Seal Bacterial Version Mechanical seal Cell Version Magnetic coupling
• Gassing options Bacterial Version Rotameter (options: mass
flow valve and oxygene supplementation
Cell Version Rotameter and gas mix (options: mass flow valve)
• Number of pump 3 (option: +1 pump)• Parameters °C, RPM, pH, pO2, AF, gas
flow, level, pressure, weight, feed, analog pump
Specification
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Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
Infors GmbH
Dachauer Str. 6D-85254 EinsbachT +49 (0)8135 83 33F +49 (0)8135/83 [email protected]
Infors UK Ltd
The Courtyard Business Centre, Dovers Farm Lonesome Lane, ReigateSurrey, RH2 7QT, VK T +44 (0)1737 22 31 00F +44 (0)1737 24 72 [email protected]
Infors Sarl
2, rue du Buissonaux FraisesBâtiment D13FR-91300 Massy T +33 (0)169 30 95 04F +33 (0)169 30 95 [email protected]
Infors Benelux BV
Fabriekstraat 387005 AR DoetinchemP.O. Box 613NL-7000 AP DoetinchemT + 31(0)314-36 44 50F + 31(0)314-37 80 [email protected]
Infors Canada
8350 rue BombardierAnjou, QuebecCanada H1J 1A6T + 1 514 352 50 95F + 1 514 352 56 [email protected]
Infors Biotechnology (China) Co., Ltd.Room 505C, Building 106Lize ZhongyuanWangjing New Industrial ZoneChaoyang District, Peking100102 Volksrepublik ChinaT +86 10 51652068F +86 10 [email protected]
For more information please visit: www.infors-ht.com
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Terrafors-ISA solid state, in situ sterilisable bioreactor
May be subject to technical amendmentswww.infors-ht.com
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Solid State Bioreactor | Terrafors-IS
Compact, for la
boratory use
Automatic in s
itu sterilisation
Vessel with 15
L total volume
Monitoring usi
ng exit gas ana
lysis
Simple and int
uitive local cont
rol
Enhanced pro
cess understand
ing
+ CO N T RO
L +
+ A
CCURATE +
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Powerful Solid State Bioreactor
Solid State Bioreactor | Terrafors-IS
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Dimensions (WxDxH): 950 x 980 x 1650 mm Total volume: 15 LWorking volumes: 3–4 kg solids/semi-solids or 7 L liquidsSpeed range: 0.1–10 rpmTemperature range: ~5°C above coolant to 80°C (134°C for sterilisation) Standard parameters: Rotation speed/direction, tem-perature (including in situ sterilisation), feed, gas flowOptional parameters: Exit gas analysis, pressure control
Key technical data
• Production of enzymes and other biologically active compounds
• Bioremediation of soils, slurries etc.• Biofuel production through conversion of agricul-
tural waste and similar material• Wastewater remediation
Applications
For more information and your local sales office please visit:
www.infors-ht.com
Infors AGHeadoffice, Switzerland
Rittergasse 27CH-4103 BottmingenT +41 (0)61 425 77 00F +41 (0)61 425 77 [email protected]
Compact, for laboratory use Terrafors-IS is mounted on a single column with the vessel to one side and
the instrumentation to the other for minimum use of space in the laboratory.
Automatic in situ sterilisation system
The automatic in situ sterilisation system uses direct steam injection with a maximum temperature of 134°C. An optional steam generator can be mounted on the base for ease of use.
Vessel with 15 L total volume The Terrafors-IS vessel is made from 316L stainless steel and provides a total
capacity of 15 L. Maximal working volumes include 3–4 kg of solids (e.g., soil), semi-solids (e.g., sludge) or up to 7 L of liquids (e.g., oil). Two ports on the top plate are included for substrate addition/removal, and sampling.
Monitoring using exit gas analysis With the additional INFORS HT Gas Analyser the evolution of CO2 can be
related to rates of substrate utilisation and parameters such as respiratory quotient (RQ) determined using oxygen and carbon dioxide ratios. These can, in turn, be used to e.g. control feed rates.
Simple and intuitive local control Terrafors-IS is equipped with a high-performance touch screen controller.
The responsive touch screen features a simple and intuitive local op-eration and allows easy access to all functions. Peripherals such as the INFORS HT Gas Analyser for exit gas measurement and analysis can be readily connected to the system.
Up to 24 parameters: Rotation speed/direction; Temperature (including
in situ sterilisation); Feed; Gas flow; + several free channels, e.g., for exit gas analysis (O2 and CO2) using the INFORS HT Gas Analyser or for pressure measurement and control up to 2 bar.
Enhanced process understanding Terrafors-IS can be linked to the Parallel Bioprocess Control Software Iris
for data logging, customisable feedback control, and data analysis. The Iris Software allows automatic rotation sequences, collection of off-gas sam-pling with automatic analysis and provides for integration of off-line data. Thus Iris is the centre for true biological process understanding and control.