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A Food Composition Database for Bangladesh with Special reference to Selected Ethnic Foods Final Report PR #11/08 By Sheikh Nazrul Islam, Principal Investigator Md. Nazrul Islam Khan, Co-Investigator M. Akhtaruzzaman, Co-Investigator Institute of Nutrition and Food Science University of Dhaka November 2010 This study was carried out with the support of the National Food Policy Capacity Strengthening Programme

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Page 1: A Food Composition Database for Bangladesh with Special ...fpmu.gov.bd/agridrupal/sites/default/files/Final... · A Food Composition Database for Bangladesh with Special reference

A Food Composition Database for Bangladesh with Special reference to

Selected Ethnic Foods

Final Report PR #11/08

By

Sheikh Nazrul Islam, Principal Investigator

Md. Nazrul Islam Khan, Co-Investigator M. Akhtaruzzaman, Co-Investigator

Institute of Nutrition and Food Science

University of Dhaka

November 2010

This study was carried out with the support of the

National Food Policy Capacity Strengthening Program me

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This study was financed under the Research Grants Scheme (RGS) of the National Food Policy Capacity Strengthening Programme (NFPCSP). The purpose of the RGS was to assist in improving research and dialogue within civil society so as to inform and enrich the implementation of the National Food Policy. The NFPCSP is being implemented by the Food and Agriculture Organization of the United Nations (FAO) and the Food Planning and Monitoring Unit (FPMU), Ministry of Food and Disaster Management with the financial support of EU and USAID.

The designation and presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of FAO nor of the NFPCSP, Government of Bangladesh, EU or USAID and reflects the sole opinions and views of the authors who are fully responsible for the contents, findings and recommendations of this report.

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Executive Summary

A food composition database (FCD) provides essential information on the nutritive value

of foods for which updated data is available. FCD is required for formulating diets,

calculating the nutritive value of diets, quantitatively assessing diets for individuals or

different population groups and for diet therapy and management. FCD can also be used

as a guideline for food analysis in estimating nutrient levels of foods prior to actual

analysis. This is particularly useful in nutrition labeling. On the whole FCD provides the

basis for planning food, nutrition and health related policy tools. Bangladesh is in the

process of revisiting the existing FCD, with the purpose of updating and analyzing the

nutrient composition of general and ethnic foods. Presently, the nutrient values of many

of the foods have been obtained from food composition tables prepared by the Institute of

Nutrition and Food Science (INFS), University of Dhaka and Helen Keller International

(1988), wherein most of the nutrient data is based on the analysis that was car long ago,

and some that was drawn from the FCD of neighbouring countries, notably India. In the

ensuing decades, major changes have occurred in the nature and complexity of the food

chain as also in the environment, soil composition, cropping patterns and intensity. Little

is known about the nutrient composition of most of the new high yielding varieties of rice,

wheat, maize, potatoe s, fruits, vegetables, fish and livestock that have become part of

the nation’s production and consumption systems. Also, the nutrient composition of the

indigenous foods grown and consumed in the Chittagong Hill Tracts (CHT) and other

tribal areas is not known. To prepare dietary guidelines and determine standard dietary

intake, the true nutrient content of these foods needs to be known.

The present study has been undertaken to prepare a FCD with special reference to

general and ethnic foods. The study was designed to (i) conduct a comprehensive food

consumption survey (CFCS) among general and ethnic populations to identify the key

food items and (ii) carry out analysis for nutrient values of key food items. The survey

was conducted on a randomly selected sample of 2015 households covering 1210

general and 805 ethnic households. A total of 75 general and ethnic foods have been

selected for analysis of 22 nutrients and calorie. Validated standard and AOAC methods

have been employed for analysis of the nutrients in the selected 75 key foods. The

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nutrient profiling comprised proximate principles such as protein, fat, carbohydrate,

dietary fiber, phytate, selected micronutrients and related compounds such as total

carotenoids, β-carotene, vitamin C and minerals. Nutrient data obtained have been

compared with reported values published in different articles and books, most of which

are consistent with the reported value. The data has been compared with the FCT and

the Thai FCT. This food composition database would serve as an important primary

source for updating FCT in Bangladesh which is an essential tool in food policy planning

and program.

Keywords: Food Composition Database, General food, Ethnic food, Bangladesh

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Contents

Executive summary 2

Chaper s 4

Tables 6

Figures 7

Abbreviations

8

Chapter 1 Introduction

1 Introduction 10

1.1 Background 10

1.2 Rationale of the study 11

1.3 Objectives and approach 14

Chapter 2 Materials and Methods

2 Materials and methods 16

2.1 Identification of key food items through CFCS 16

2.1.1 Comprehensive Food Consumption Survey (CFCS) 17

2.1.1.1 Sample size determination 18

2.1.1.2 Selection of general households 19

2.1.1.3 Selection of ethnic household 21

2.1.1.4 Questionnaire design, enumerator training and pre-testing 25

2.1.1.5 Comprehensive food consumption survey 26

2.1.1.5.1 Data collection, management and analysis 26

2.1.2 Focus group discussions (FGD) 27

2.1.3 Lifestyle characteristics of the general and ethnic population 28

2.1.4 Selection of key food items 28

2.2 Analysis of nutrients in key foods 35

2.2.1 Food sampling protocol 35

2.2.1.1 General food sampling protocol 37

2.2.1.2 Ethnic food sampling 39

2.2.2 Procedure for food sample collection 40

2.2.3 Identification of collected food samples 40

2.2.4 Sample preparation for analysis 41

2.2.5 Chemicals 43

2.2.6 Methods of nutrient analysis 43

2.2.6.1 Analysis of moisture 45

2.2.6.2 Estimation of protein 45

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2.2.6.3 Estimation of total fat and fatty acids 45

2.2.6.4 Estimation of ash content 45

2.2.6.5 Analysis of crude fibre and dietary fibre 46

2.2.6.6 Analysis of phytic acid 46

2.2.6.7 Calculation of carbohydrate and energy 47

2.2.6.8 Analysis of vitamin C 47

2.2.6.9 Analysis of carotenoids 47

2.2.6.10 Analysis of β-carotene 48

2.2.6.11 Analysis of mineral profile 48

2.2.7 Quality assurance programme (QAP) 48

Chapter 3 Results and Discussion

3 Results and Discussion 50

3.1 Key food identification 51

3.1.1 Comprehensive Food Survey (CFCS) 51

3.1.2 Focus group discussions (FGDs) 60

3.1.3 Lifestyle characteristics of general and ethnic people 66

3.1.4 Identification of key foods 74

3.1.5 Selection of key food 76

3.2 Collection of food sample 82

3.3 Nutrient composition of key foods 85

3.3.1 Proximate Nutrients 86

3.3.2 Water in key Foods 87

3.3.3 Dietary fiber 87

3.3.4 -Phytate content 87

3.3.5 Vitamins and Minerals in key Foods 88

Key Findings 103

Policy Implications and Recommendations 105

Policy Recommendat ions 107

Future Research 109

Conclusion 109

Acknowledgements 110

References 112

Research team 116

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Tables page

Table 2.1 Ethnic household selection representing 70% of ethnic population 22

Table 2.2 Focus group discussions 28

Table 2.3 Food items consumed by only general people (percent frequency ≥ 5%) 31

Table 2.4 Food items consumed by only ethnic households (percent frequency ≥ 5%) 32

Table 2.5 Food items commonly consumed by both General and Ethnic people

(Percent frequency ≥ 5% households)

33

Table 2.6 Ethnic food items listed from ethnic CFCS and FGDs 34

Table 2.7. Nutrients analysed and the analytical techniques employed 44

Table 3.1 Location and descriptive of CFCS the data collection among the native

local/Indigenous population

54

Table 3.2 Location and descriptionof CFCS data collection among the ethnic population 55

Table 3.3 FGDs settings 60

Table 3.4 FGD outcome: Food consumption pattern of the Marma, Chakma, Tanchanga and

Tripura communities

61

Table 3.5 Socioeconomic profile of general households 68

Table 3.6 Food security by households’ type in general population 69

Table 3.7 Morbidity and its treatment by household type in general population 70

Table 3.8 Socioeconomic profile of ethnic households 71

Table 3.9 Food security of ethnic tribes 72

Table 3.10 Morbidity and its treatment by ethnic tribes 73

Table 3.11 Key food list consumed by both the native general and *ethnic people 78

Table 3.12 Exclusive ethnic food list 79

Table 3.13 Proximate nutrient composition of cereals and leafy vegetables 90

Table 3.14 Vitamin C, carotenoids and micromineral composition of cereals and leafy

vegetables

91

Table 3.15 Macromineral composition of cereals and leafy vegetables 92

Table 3.16 Proximate composition of roots & tuber, non-leafy vegetables and fruits 93

Table 3.17 Vitamin C, carotenoids and micromineral composition of roots & tuber, non-leafy vegetables

94

Table 3.18 Macromineral composition of of roots & tuber, non-leafy vegetables 95

Table 3.19 Proximate composition of fish, egg and meat 96

Table 3.20 Micromineral composition of fish, egg and meat 97

Table 3.21 Macromineral composition of fish, egg and meat 98

Table 3.22 β-carotene content in general and ethnic foods 99

Table 3.23 Dietary fiber in key food items 100

Table 3.24 Phytic acid content in key food items 101

Table 3.25 Comparision of protein value in the present FCD with IFCT, DKPM, Thai

FCT

102

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Figures page

Figure 2.1 Sampling plan of general households 20

Figure 2.2 Sampling plan for ethnic households 23

Figure 2.3 Geographical locations of ethnic CFCS 24

Figure 2.4 Multi-regions sampling plan for general food sample 38

Figure 2.5 Multi-regions sampling plan for ethnic food 39

CFCS activities 52

Figure 3.1 Distribution of general and ethnic households 56

Figure 3.2 Distribution of selected general households by division and household type 57

Figure 3.3 Distribution of ethnic households by districts 58

Figure 3.4 Distribution of ethnic households by tribes 59

FGDs activities 62

Figure 3.5 Number of food item consumed by population type 74

Figure 3.6: Distribution of common food item consumed by ≥5% HH 75

Figure 3.7 Distribution of ethnic food of food items consumed by ≥5% HH 75

Figure 3.8 Distribution of general food items consumed by ≥5% HH 75

General key foods 80

Ethnic key food 81

Ethnic food collection activities 82

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Abbreviations

AOAC Association of Official Analytical Chemists

CFCS Comprehensive Food Consumption Survey

CHT Chittagong Hill Tracts

CV Co-efficient of Variance

DAE Department of Agricultural Extension

DKPM

EP

Dhesio Khadder Pustiman

Edible Portion

EU European Union

ES External Standard

FAO Food and Agriculture Organization of the United Nations

FCDB Food Composition Database

FCT Food Composition Tables

FGDs Focus group discussions

HKI Helen Keller International

HYV High Yielding Varieties

IFCT Indian Food Composition Tables

INFS Institute of Nutrition and Food Science

IS Internal Standard

NFCD National Food Composition Database

SRM Standard Reference material

SEM Standard Error of Mean

TAT Technical Advisory Team

TDF Total Dietary Fiber

USAID United States Agency for International Development

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Chapter 1

Introduction

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1 Introduction

A food composition database (FCDB) provides detailed information on the nutrient

composition of foods. FCDBs provide values for energy and nutrients (e.g. protein,

vitamins and minerals) and other important food components or bioactive compounds

that are important for human nutrition. This includes the nutrient profile of key foods

commonly taken by the population. The key food list comprises the local staples,

cereals, fish, meat, vegetables, fruits, milk and others. The nutritive values are either

based on chemical analysis which are carried out in analytical laboratories or are

estimated from other appropriate data. The earliest known food composition table

was produced in 1818 (Somogyi, 1974). The current knowledge of nutrition is still

incomplete, and studies are still required, often at ever increasing level of

sophistication, into the composition of foods and the role of these components and

their interactions in health diseases (Greenfield and Southgate, 2003a). Food

composition database will serve to address the basic need for nutrient information,

public health problems in the country, the current knowledge in nutrition, and for food

safety and toxicity.

1.1 Background

Food is one of the essential components for human survival. Good health needs a

balanced diet. In order to achieve this, the nutrient composition of most frequently

consumed foods has to be made well-known and available to the mass population.

Food composition database is of great importance in health and nutrition. It is used in

research studies dealing with the effects of diets on health, reproduction and

development. There is a significant relationship between diet and health and

diseases. Lack of proper dietary habits contributes to the development of many

diseases. In this regard, there is a worldwide call for updating or establishing the

Food Composition Database. Many countries, particularly in the developing world,

lack the resources needed for setting up a national food composition programme.

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Some countries are collaborating on food composition analyses among the

institutions in their own country and in the region. Accordingly Bangladesh has

undertaken steps in generating its own FCD.

Bangladesh is an agriculture based country. Agriculture produces around 90% of its

food need including cereals and vegetables (FAO/WFP CFSAM 2008; WFP, 2010). It

has been blessed with high yielding varieties (HYV) of rice, and plenty of vegetables

and fruits. There are 141 varieties of leafy vegetables (commonly known as shak)

and 25 varieties of non-leafy vegetables in Bangladesh (Maksuda, 2010). Among the

leafy vegetables, 97 items are identified as ethnic varieties, and the rest are

consumed by both the general and ethnic people. A good number of shaks grow as

weeds or during cultivation of other crops. Many of the poor and landless people

depend on these indigenous foods (SANFEC, 2005). Several the indigenous fruits

and vegetables are known to be nutritionally rich with vitamins and minerals. The

biologically rich open water bodies include 260-500 species of inland fish, and some

seventy five of these species are regularly consumed by poor communities (Minkin et

al, 1997; Rahman and Minkin, 2003; Rahman, 2005; FAO/CINE, 2009 ). The nutrient

content of these foods should be incorporated into the food composition table as a

valuable source of information on nutrition and food diversity. The nutritive values of

these abundantly produced foods as well as the ethnic foods needs to be analyzed

and incorporated in the Food Composition Database.

1.2 Rationale of the study

The national food intake pattern in Bangladesh is dominated by cereals contributing

up to 74-76% of total dietary energy as against the internationally accepted value 54-

55% for developing countries (WHO/FAO, 2003; WHO/FAO, 2004; Murshid et al.,

2008; Yusuf et al, 2009). Vegetables comprise one-fifth of total diet for rural people.

Protein and micronutrient rich foods account for less than 10 percent of the rural

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person’s diet. Intake of vegetables and fruits has increased considerably. It is still

very low, although their consumption is vital for a diversified and nutritious diet (BBS,

2007). The high intake of cereal based food and low intake of micronutrient rich foods

results in an unmbalanced diet and causes different health disorders. Diets rich in

vegetables and fruits contribute to micronutrients that have specific antioxidant

functions and many of which reduce the risk of many health disorders including

cardiovascular complications, diabetes related damage, cancers (Connealy, 2008;

Liu, 2003; Kaur and Kapoor, 2001), even HIV infection (Oguntibeju, 2009; Baeten et al,

2001). Additionally they provide phytochemicals that have marked health significance.

Therefore, it is important to identify the food sources of various nutrients that are

required for the maintenance of good health.

Over the last decade, food composition activities have increasingly been undertaken

by several agencies and programmes for its ever growing importance. Many national,

regional and international organizations recognize its significance. The food

composition data are used primarily for the planning, assessment and establishment

of human energy and nutrient requirements and intakes. Its importance is versatile.

It is required for nutrition planning and in agriculture, health and nutrition assessment;

formulation of national; institutional and therapeutic diets; nutrition education and

training; formulation of food based dietary guidelines; research on nutrition,

agriculture and epidemiology; product development; nutrition labeling; setting food

standards and establishing food safety regulations.

Until now, data on nutrient values have been obtained from food composition tables

(FCT) prepared for Bangladesh by the Institute of Nutrition and Food Science,

University of Dhaka (INFS, DU, 1986) and Helen Keller International (HKI, 1988).

Most of the nutrient data in these FCT were analyzed long ago with uch of the data

borrowed from neighboring countries. Moreover, the nutrient composition of ethnic

foods is not available in the Bangladesh food composition table. With the increasing

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concern of the relationships between diet, food habits and degenerative lifestyle

diseases, there is increased interest in food composition data. At the same time,

there is a call for attention to the major limitations in the available data and to support

a variety of research activities in this area (Greenfield and Southgate, 2003a),

particularly in food security mapping. This would help to bridge the lack of

information on the nutrient and non-nutrient content of different foodstuffs consumed

by different populations and subgroups including ethnic populations.

Further, changes in the food chain due to emergence of high yielding varieties (HYV)

newer foods and changes in soil composition (due to environmental changes,

increased use of fertilizers and crop intensity) have resulted in possible changes in

the composition of nutrient in the foods now being grown. The food chain of the

country has been modified during the last decades. Nutritive values of these local

food items need to be analyzed and incorporated in the food composition database.

All these facts call for a renewed look and analysis of the most frequently consumed

foods.

It is time to prepare a Food Composition Database with nutrient data through

analysis of general, ethnic and relatively newer foods. Such a Food Composition

Database will help in formulating dietary guidelines for different people to meet their

nutrient requirements. This is also in line with one of the key areas of intervention of

the National Food Policy Plan of Action (2008-2015).

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1.3 Objectives and approach

Considering the importance of having a National Nutrient Database, this study aimed

to prepare a Food Composition Database with reference to general and ethnic

foods. To this end, the study was designed to:

� identify the most frequently consumed foods of the general and ethnic

people of Bangladesh through a comprehensive food consumption

survey(CFCS);

� prepare a key foods list that contributes 75% of any one nutrient need

(key food list);

� analyse macronutrients, micronutrients, and anti-nutrients in the selected

key foods (nutrient value of food);

� develop a comprehensive National Food Composition Database (NFCD)

with the analytical results obtained; and

� provide recommendations for food policy planning and program.

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Chapter 3

Materials and Methods

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2 Materials and methods

Food composition database gives detailed information on the nutrient composition of

foods providing values of nutrients, energy and other important food components for

each food. Nutrient values can be obtained by chemical analysis of foods in

laboratories (direct method) or can be estimated from published literature,

unpublished laboratory reports (indirect method) or by combining data of direct and

indirect methods containing lab analytical values together with the values taken from

the literature and other database as well as imputed and calculated values.

Therefore, the types of food composition data are of original analytical values (lab

generated analytical, published or unpublished), imputed values (analytical values

obtained for a similar food), calculated values, borrowed values and presumed

values.

This study has aimed to prepare a food composition database with nutrient

composition of general and ethnic foods based on nutrient data generated by

laboratory analysis of key foods. Thus, this study comprised-

• Identification of key food items through Comprehensive Food

Consumption Survey (CFCS) and

• Analysis of nutrients in the selected key foods.

2.1. Identification of key food items through CFCS

The key foods provide 75% of daily nutrient need (Haytowitz et al, 1996; 2000;

2002). Identifying and prioritizing the most significant foods and nutrients for sampling

and analysis is essential in preparation of national food composition database. Key

foods can be listed by data obtained from food consumption surveys that determine a

food's relative nutrient contribution to the diet of a population. Diet has been

implicated in the etiology of chronic diseases in many populations. It is further noted

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that some food source as those consumed by specific population contribute

substantially to the nutrient of their diets. Therefore, alternative methods of collecting

information, such as small localized surveys and interviews were carried out.

Key food items were indentified through a Comprehensive Food Consumption

Survey (CFCS) among the general and ethnic population of Bangladesh.

General and Ethnic Foods

In this study, general foods are referred to those foods which are consumed by local

general people (Rahman et al, 2001; Rashid et al, 2007) who constitute the majority

of the Bangladeshi population. Ethnic foods are those foods which are consumed by

ethnic tribal people who are the inhabitants of the Chittagong Hill Tracts (CHT) region

and other specific locations in Bangladesh.

The majority of the foods that have been analysed for the nutrient content are

commonly consumed by both the general and ethnic people of Bangladesh. Some

foods which are uncommon in the food consumption list have also been included for

analysis of their nutrient profile.

2.1.1 Comprehensive Food Consumption Survey (CFCS)

Food consumption surveys form the basis for food intake surveys or dietary surveys.

The aim of the CFCS was to collect food consumption data of the general and ethnic

population that included the types and amounts of food intake, frequency of intake

and dietary practices. CFCS was also conducted to prepare a comprehensive

database that would be useful for food safety risk assessmen. It would also provide a

valuable resource for health protection and public health policy planning.

In this study, the comprehensive food consumption survey (CFCS) was conducted to

collect data on the diversity of food items that are most frequently consumed by

general and ethnic people in Bangladesh. The aim of this survey was to obtain a key

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food list that includes the most frequently and commonly consumed foods by both

groups of population. In addition, the CFCS also collected information on the lifestyle,

socioeconomic information, food security and health related knowledge of the

general and ethnic people.

The CFCS was conducted among a cross-sectional population of adults and older

groups of population of general and ethnic origins (Kuhnlein et al, 2006). A pretested

questionnaire was used to conduct the survey. Pretesting was performed by trained

enumerators in cluster mapping locations.

2.1.1.1 Sample size determination

In the study, households were taken as the sampling unit. This is based on the

principle that in most cases, food is first purchased in the household and then

consumed by the members of the household. To determine the sample size required

the following statistical formula was used:

n = {Z2P(1-P)}/ d 2 where,

n = Minimum sample size

P = Expected proportion of the household consuming the diversified food items

Z = Standard error corresponding to a given confidence level

d = Precision of the estimate which is considered to be 0.05 at 95% confidence level.

Considering the prevalence of diversity in food consumption by the households and

by the individuals at 0.15% and the standard scores of the estimate at 95%

confidence level with precision of 0.05, the above equation gave a value of sample

size of 196 households equivalent to 200 households as minimum sample size from

each of the six divisions of Bangladesh. Thus, it comprised a total of 1200 general

households. It was selected to get the percentage of households consuming the

specific food items throughout the year by the general population in Bangladesh.

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2.1.1.2 Selection of general households

In selecting the 1200 general representative households, a three stage sampling

technique was used.

Bangladesh, administratively, is divided into six divisions. In selecting the 1200

households, 200 households were selected from each of the six divisions in the first

stage. To select the 200 households from each division in the second stage, two

districts were randomly selected from each of the six divisions and then 100

households were selected from each of the selected 12 districts. Finally in the third

stage, 50 households from urban setting (district city) and 50 households from

multiple rural settings under the same district were randomly selected. The

household sampling plan is presented in the following diagram.

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Figure 2.1: Sampling plan of general households

Chittagong HH # 200

Rajshahi HH # 200

Barisal HH # 200

D1

Dhaka HH # 200

Khulna HH # 200

Sylhet HH # 200

D1 D2 D1 D2 D1 D2 D2 D2 D1 D2 D1

Bangladesh HH # 1200

R U R U R U R U R U R U R U R U R U R U R U R U

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2.1.1.3 Selection of ethnic households

Twenty eight tribes comprising 2,33,417 number of households have been living in

Bangladesh (BBS survey, 1991). Among them, the tribes which have at least 1.5%

representation in the total ethnic households living in Bangladesh were taken into the

study. This stood at 11 tribes that had ≥5% representation in the total tribal population

living in Bangladesh. These included Marma, Chakma, Tanchanga, Tripura, Bam,

Murang, Monipuri, Khashia, Shaotal, Garo and Hajong, which comprise 1,64,667

households representing 70.54% of total ethnic households living in Bangladesh. Ethnic

people of the 11 tribes live in the four divisions of Bangladesh namely Dhaka (Durgapur

Upazilla under Netrokona districts), Sylhet (Kamalgonj Upazilla under Moulavi Bazar

district), Chittagong (Khagrachari, Rangamati and Bandarban Sadar Upazilla) and

Rajshahi (Godagari Upazilla under Rajshahi district).

On the basis of probability proportions (PPS) to the size, a total of 400 households were

selected from the 11 tribes. In selecting the households on the PPs basis, the

household numbers, in the case of some tribes, were found to be less than 30 in

number. To have the normality in the distribution, the household’s size was increased to

at least 30 in number. In doing this, the total number of households to be selected stood

at 500 households. The 500 households were selected randomly from the 11

representative tribes. The selected ethnic household list by tribes is given in table 2.1

and figure 2.2. They were interviewed using a pretested questionnaire.

Following the presentation of the study’s interim findings at the Workshop in Rangamati,

CHT on 18th March, 2010 a careful review showed that there was need to have an

appropriate inclusion of ethnic foods for nutrient analysis. Suggestions were also made

by some of the CHT ethnic members. It was, therefore, decided to include some more

ethnic food items so as to have the nutrient profile of an adequate number of ethnic

foods. In consultation with FAO Technical Assistance Team (TAT) members, a CFCS

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was, therefore, conducted on another 300 ethnic households in Khagrachari and

Rangamati districts of CHT. It was undertaken among Marma, Chakma, Tripura and

Tangchaga ethnic community during March and April, 2010. Thus the CFCS on ethnic

households was carried out on a total of 805 households. The selection criteria

employed to recruit the ethnic households are described in table 2.1 and figure 2.2 and

2.3.

Table 2.1: Ethnic household selection representing 70% of ethnic population

Tribe name No. of household in respective tribe

PPS-Households in respective tribe

Projected PPS-Households in respective tribe

Targeted Households selected in the study

Chakma 44730 108 136 238

Marma 29137 71 88 171

Tanchanga 4043 10 12 51

Tripura 15220 37 46 87

Bam 2681 7 8 30

Murong 4273 10 13 30

Monipuri 3559 9 11 25

Khasia 7500 19 23 25

Santal 36406 88 111 89

Garo 12867 31 39 31

Hajong 4251 10 13 28

Total households 1,64,667 (>70%) 400 500 805

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Figure 2.2: Sampling plan for e thnic household s

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Figure 2.3: Geographical locations of ethnic CFCS

Moulavi Bazar

Mymensingh

Rajshahi

Khagrachari

Rangamati

Bandarban

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2.1.1.4 Questionnaire design, enumerator training and pre-testing

Questionnaire design: The major components included in the questionnaire were- types

of food consumed by the households throughout the year, socioeconomic profile, family

food security, nutritional knowledge, knowledge on nutritional deficiency diseases etc of

the projected households. This stand that whole process of collecting information on

food items commonly consumed by the household throughout the year, socioeconomic

condition and other lifestyle factors related to questionnaire. It was conducted through

direct interview to the household’s respondent during the survey. A semi precode

formatted questionnaire was used as the basic data collection tool to get the household

information. Considering the importance of the study in the national context and its

objectives, information on the variable collected were meticulously included in the

questionnaire, discussed with the Technical Advisory Team (TAT) members and

carefully examined so that all the relevant information were taken and recorded during

the comprehensive consumption survey.

The questionnaire was designed in the light of experience achieved from the National

Nutrition Survey and various other large scale surveys conducted in Bangladesh

focusing on the required variables to answer the objectives as well as purpose of the

study. The questionnaire was field tested prior to actual use and was modified on the

basis of the feed-back received from the field tests.

The questionnaire and selection of survey site were finalized and approved in

consultation with Technical Advisory Team (TAT) members of this Programme.

Enumerator recruitment and training: A team consisting of four enumerators with one

supervisor were recruited and trained to conduct the field survey. All the enumerators

recruited were university graduates and postgraduates. In the five member’s team, two

enumerators belonged to general community and three were ethnic who were fluent in

speaking and understanding the general people’s language as well as the tribal people’s

language. More ethnic members were recruited because they were familiar with the

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difficulties in tribal locations where the ethnic people are mostly concentrated as well as

to facilitate the data collection within the stipulated time. Initially, all field staffs received 7

days’ orientation training consisting of familiarization of the questionnaire through guided

readings and field trials.

Pretesting questionnaire: The enumerator team spent a considerable time in the office

and at the field-testing sites in practicing the techniques of recording types of food

consumed by the household throughout the year and the other related variables included

in the questionnaire as well as the related data collection activity. Fifty households

comprising general and ethnic people were interviewed in pretesting the questionnaire.

2.1.1.5 Comprehensive food consumption survey

To identify the most common food items consumed by the general and ethnic people,

2015 households was selected comprising 1210 general and 805 ethnic households that

were interviewed with a precoded and pretested questionnaire. Though there is

disproportionate distribution of general population in rural and urban locations, in order

to obtain the maximum diversity in consumption of different food items, an equal number

of households were selected from both the urban and rural locations. Further, to get the

factual data on food consumption in the rural and urban population, a weighted food

frequency was calculated giving the actual weighted representation of the rural urban

population proportion in the country.

2.1.1.5.1 Data collection, management and analysis

Data collection: Data were collected from the selected locations and households

through home visits during the period January to May 2009 and during April to May,

2010. To get the information related to food purchase, consumption and other variables,

the household head (male) and the spouse were interviewed. Every day, the collected

information/data was checked, coded and cross checked by the interviewers and finally

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by the supervisor at the field sites in order to avoid any misreporting. Any confusion

arising out of this matter was settled on the following day during subsequent home visits.

This process of scrutinizing the data was performed during the entire period of CFCS.

Data management and analysis: The questionnaire was edited and entered into

SPSS program. Data entry was done by the computer data entry personnel of INFS, DU

and this was followed by an extensive period of logical checking to identify any error in

data entry, which were then corrected by consulting the original questionnaires.

2.1.2 Focus group discussions (FGDs)

The focus group is a type of group interview (http://www.extension.iastate.edu/publications/

pm1969b.pdf). It provides qualitative approaches to research aiming to obtain in-depth

information on concepts, perceptions and ideas of a group on certain specific topic in

short time at relatively low cost. The FGD supplements the survey data. In case of

health and nutrition, it is primarily done to get information regarding the lifestyle, food

consumption, food security, health and nutrition knowledge of a community. The

activities of conducting a focus group include- identification of the objectives of the focus

group discussions, preparation of questions, selection of participants, selection of

location and facilitator, note-taker and planning of session. It produces high quality data

if it is employed for the right purposes using the right procedures.

The FGD comprises a group of approximately 6-12 participants with key informants such

as community leaders and a critique, and the discussion may last for one hour to one

and half hour (IDRC, http://www.idrc.ca/en/ev-56615-201-1-DO_TOPIC.html). It is an important tool for

acquiring feedback regarding the topic, and it facilitates the enumerators to talk to the

people in a more natural setting than a one-to-one interview. In presence of the critique,

the participants and key informants are directly asked about their perceptions, opinions,

beliefs and attitudes towards a particular topic. Their responses are discussed, criticized

and recorded.

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It has a high apparent validity - since it is easy to understand, and the results are

believable. FGD is relatively easy to assemble, good for getting rich data in participants'

own words and developing deeper insights, good for obtaining data from children and/or

people with low levels of literacy, identifying factual errors or extreme views. Its

limitations are -the responses of each participant are not independent, a few dominant

focus group members can skew the session. Focus groups require a skilled and

experienced moderator and the data analysis requires expertise and experience.

In the present study, FGD was conducted among the ethnic communities of Marma,

Chakma, Tripura and Tangchaga living in Khagrachari and Rangamati during March and

April, 2010 (table 2.2). It was carried out to obtain information on their food consumption

pattern.

Table 2.2: Focus group discussions

Division District Upazilla Time of visit Location Type of HHs

Chittagong

Khagrachari Khagrachari sadar

31/03/2010 Marma palli Marma

Rangamati Rangamati sadar

03/04/2010 Chakma palli Chakma

Rangamati Rangamati sadar

08/04/2010 Tanchanga para,

Tanchanga

Khagrachari Khagrachari sadar

21/04/2010 Tripura para Tripura

2.1.3 Lifestyle characteristics of the general and ethnic population

Although the primary aim of the CFCS was to obtain the information on the food

consumption pattern of the general and ethnic people, information on their lifestyle such

as socioeconomic profile, food security and morbidity and care taken for it were also

collected, analysed and addressed.

2.1.4 Selection of key food items

It is documented that the key foods contribute up to 80 percent of any nutrient, but the

total nutrient contribution of key foods in a diet accounts for approximately 90 percent of

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the nutrient contents of the diet. In selecting the key foods, priority was given to those

foods that contribute primarily to the energy anf key nutrients of the diet. In addition,

considerations were given to- the basic need for nutrient composition, public health

problems in the country, current knowledge on nutrition and toxicity, availability of

existing data, existence of adequate analytical methods, and feasibility of analytical

works. Special focus was given to the distribution of nutrients in foods with emphasis on

β-carotene, vitamin C, calcium and iron content. Importance of food trading was also

considered in making the key food list (Greenfield and Southgate, 2003e).

Analysis of CFCS data indicated that food items consumed by the ≥5% households

included a list of 120 foods comprising 20 foods consumed only by the general people

(table 2.2), 46 foods consumed only by ethnic people (table 2.3) and 54 common food

items consumed by both the general and ethnic population (table 2.4).

The study undertook preparation of a database with nutrient composition of 50 key food

items. In preparation of the list of 50 food items out of 120 items, the following criteria

were used:

� food items that were consumed by ≥15% of the households were included in the

key food list.

� some of the ethnic foods were excluded though consumed by >15% households

of the ethnic population on the basis that these are being consumed by a very

minor group of population. The above exclusion criteria condensed the food list

to 70 food items.

� further to make the list to 50 items, the foods containing poor micronutrients (less

or no β-carotene) were excluded.

� thus the key food list included 50 food items.

The 50 key food items were initially selected in consultation with Technical Assistance

Team (TAT) members. Later in compliance with the recommendation made by some

ethnic participants at Rangamati workshop for inclusion of more ethnic tribal foods, a

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critical review and discussion were made with TAT members, and it was decided to

survey on additional 300 ethnic households to include an adequate number of ethnic

foods for nutrient analysis.

Inclusion of additional ethnic foods made the key food list of 75 food items. This revised

key food list comprised 53 general food items (most of which are consumed by ethnic

people) and 22 ethnic foods.

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Table 2.3: Food items consumed by only general peop le (percent frequency ≥ 5%)

Sl no English name Bengali name Scientific name Urban Rur al weighted % frequency

Leafy vegetables

1 Spleen Amaranth Data shak Amaranthus dubius 14

2 Jute Pat shak Corchorus capsularis 11

3 Swamp Morning-glory Kalmi shak Ipomoea aquatica 17

4 Coco-yam Sobuj kochu shak Colocasia esculenta 5

Non-Leafy vegetables

5 Spleen Amaranth Data Amaranthus dubius 13

6 Bean Broad Makhon shim Canavalia gladiata 5

7 Drumstick Shajna data Moringa olefera 7

Fruits

8 Apple Apel Pyrus malus 7

9 Bullocks Heart Atafol Annona reticulata 5

10 Water melon Tormuz Citrullus vulgaricus 22

Fish and Meat

11 Sunfish Mola mach Mola mola 14

12 Taki fish Taki mach Channa puncpatus 10

13 Bailla Bele mach Awaous guamensis 17

14 Ganges River Gizzard Shad Chapila mach Gonialosa manmina 6

15 Zig-zag eel/Tire track eel Baim mach Mastacembelus armatus 5

16 Hilsha Fish Ilish mach Tenualosa ilisha 7

17 Chingri mach Shrimp Macrobrachium rosenberghii 29

18 Striped dwarf catfish Tengra Fish (Taja) Mystus vittatus 23

19 Beef Garor mangsha Beef cattle 26

20 Chicken egg (farm) Murgir dim (farm) Gallus bankiva murghi 45

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Table 2.4: Food items consumed by only ethnic house holds (percent frequency ≥ 5%)

Sl. no English name Bengali/Local name

Scientific name Urban Rural weighted % frequency native

general people

% frequency of Ethnic people food

consumption

CEREALS 1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 99 34 2 Lentil (deshi) Masur dal Lens culinaris 78 35

LEAFY VEGETABLES 3 Joseph’s Coat Lalshak Amaranthus gangeticus 84 49 4 Bottle Gourd Lau shak Lagenaria siceraria 47 42 5 Indian spinach Pui shak Basella alba 64 28 6 Radish Mula shak Raphanus sativus 7 Spinach Palong sag Spinacea oleracea 41 17 8 Coco-yam Sobuj kochu shak Colocasia esculenta 18 17 9 Bathua Pigweed Chenopodium album 13 7

ROOTS & TUBERS 10 Potato Gol Alu Solanum tuberosum 93 93 11 Radish Mula Raphanus sativus 44 40 12 Coco-yam Sobuj kochu Colocasia esculenta 33 37

NON-LEAFY VEGETABLES 13 Egg plant Begun Solanum melongena 81 80 14 Bean Shim Dolichos lablab 70 75 15 Cabbage Badha Kopi Brassica oleracea var. capitata 80 58 16 Cauliflower Foolkopi Brassica oleracea var. botrytis 90 74 17 Cow pea Borboti Vigna catjang 38 8 18 Cucumber Shasha Cucumis sativus 20 21 19 Folwal Potol Trichosanthes dioica 49 16 20 Gourd (Ash) Chal kumra Benincasa cerifera 31 21 21 Bitter Gourd Karola Momordica charantia 43 42 22 Sweet pumpkin Misti kumra Cucurbita maxima 40 39 23 Kakrol Kakrol Momordica cochinchinensis 20 8 24 Ladies finger Dherosh Abelmoschus esculentus 43 24 25 Bottle gourd Lau Lagenaria siceraria 68 56 26 Snake gourd Chichinga Trichosanthes anguina 53 19 27 Jackfruit (immature) Kacha kathal Artocarpus heterophyllus 8 23 28 Green papaya Kacha papay Carica papaya 30 27 29 Plantan (green) Kacha kola Musa paradisiaca 12 18 30 Tomato (green) Kacha tomato Lycopersicon lycopersicum 21 33 31 Yam Stem Kachur data/loti Colocasia esculenta 28 12

FRUITS 32 Mango ripe(deshi) Paka Am Mangifera indica 66 56 33 Black berry (deshi) Kalojam Syzygium cumini 17 8 34 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 60 56 35 Banana (ripe) Paka kala Musa sapientum 29 17 36 Bitter Plum Boroi Zizyphus mauritiana 38 36 37 Pine Apple (Jaldugi) Anarash (Jaldugi) Ananas comosus 12 5 38 Tomato (ripe) Tomato paka Lycopersicon lycopersicum 61 52

FISH 39 Carp Katol mach Labeo rohita 21 7 40 Tilapia Tilapia mach Anabus testudineus 20 25 41 Dragon Fish Pangash Pangasius pangasius 44 26 42 Fry (very small) Choto puti Puntius ticho 56 27 43 Sunfish Mola mach Mola mola 11 9 44 Shrimp(dry) Chingri (shukna) Heterocarpus ensifer 7 22 45 Rohu Rui Labeo ruhita 45 35 46 Shrimp Chingri Heterocarpus ensifer 30 6

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Table 2.5: Food items commonly consumed by both Gen eral and Ethnic people (Percent frequency ≥ 5% households)

Sl no

English name

Bengali/

Local name

Scientific name Urban-Rural weighted % frequency native general people

% frequency of Ethnic people food consumption

Sl. no English name

Bengali/

Local name

Scientific name Urban_Rural weighted % frequency native general people

% frequency of Ethnic people food consumption

CEREALS 27 Folwal Potol Trichosanthes dioica 49 16 1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 99 34 28 Ash Gourd Chal kumra Benincasa cerifera 31 21 PULSE 29 Bottle Gourd Lau Lagenaria siceraria 68 56 2 Lentil (deshi) Masur dal Lens culinaris 78 35 30 Snake Gourd Chichinga Trichosanthes anguina 53 19 LEAFY VEGETABLES 31 Jackfruit immature Kacha Kathal Artocarpus heterophyllus 8 23 3 Joseph’s Coat Lalshak Amaranthus gangeticus 84 49 FRUITS 4 Bottle Gourd Lau shak Lagenaria siceraria 47 42 32 Mango ripe (deshi) Paka Am Mangifera indica 66 56 5 Indian spinach Poi shak Basella alba 64 28 33 Black berry (deshi) Kalojam Syzygium cumini 17 8 6 Radish Mula shak Raphanus sativus 38 34 34 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 60 56 7 Spinach Palong sag Spinacia oleracea 41 17 35 Banana (ripe) Paka kala Musa sapientum 29 17 8 Coco-yam Sobuj kochu shak Colocasia esculenta 18 17 36 Pineapple (jaldogi) Anarosh Ananas comosus 12 5 9 Bathua leaves Batua shak Chenopodium album 13 7 37 Bitter Plum Boroi Zyzyphus mauritiana 38 36

ROOTS & TUBERS 38 Tomato (Ripe) Tomato paka Lycopesicon lycopersicum 61 52 10 Potato Gol Alu Solanum tuberosum 93 93 FISHES 11 Radish Mula Raphanus sativus 44 40 39 Carp (small) Nala Labeo rohita 24 16 12 Coco-yam Sobuj kochu Colocasia esculenta 33 37 40 Ruhi Ruhi Labeo rohita 46 35 13 Coco-yam stem Sobuj kochu Colocasia esculenta 41 Carp Katol mach Catla catla 21 7 Non-LEAFY VEGETABLES 42 Tilapia Tilapia mach Anabus testudineus 20 25 14 Egg plant Begun Solanum melongena 81 80 43 Dragon Fish Pangash Pangasius pangasius 44 26 15 Bitter Gourd Karola Momordica charantia 43 42 44 Sunfish Mola mach Mola mola 11 9 16 Sweet pumpkin Misti kumra Cucurbita maxima 40 39 45 Silver Carp Silver Carp Hypophthalmichthys nobilis 42 13 17 Kakrol Kakrol Momordica cochinchinensis 20 8 46 Taki fish Taki mach Channa puncpatus 10 13 18 Ladies finger Dherosh Abelmoschus esculentus 43 24 47 Painted catfish Tengra (dry) Pseudolaguvis shawi 23 9 19 Green papaya Kacha papay Carica papaya 30 27 48 Fry (very small) Choto puti Puntius ticho 56 27 20 Green tomato Kacha tomato Lycopersicon lycopersicum 21 33 49 Shrimp (dry) Chingri (dry) Heterocarpus ensifer 7 22 21 Green banana Kacha kala Musa sapientum 12 18 50 Shrimp Chingri Heterocarpus ensifer 30 6 22 Bean Shim Lablab purpureus 70 75 51 Puti fish (rotten) Chepa Puntius puntio 12 16 23 Cabbage Badha Kopi Brassica oleracea var capitata 80 58 52 Laitta fish Laitta mach na 7 12 24 Cauliflower Foolkopi Brassica oleracea var. bortrytis 90 74 MEAT 25 Cow pea Borboti Vigna catjang 38 8 53 Chicken (farm) Farm murgi Gallus bankiva 40 33 26 Cucumber Shasha Curcumis sativus 20 21 54 Beef Garor mangsha Beef cattle 26 5

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Table 2.6: Ethnic food items listed from ethnic CFC S and FGDs Sl no

English name Bengali name Scientific name % household consume (n=805)

Sl no

English name Bengali name Scientific name % household consume (n=805)

CEREALS 33 na Hahnagulu na 1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 6 34 Gourd (Ridge) na na 5 2 Rice sunned* Atap chal Oryza sativa 58 35 Plantain Flower na na 14 3 Radish Mula shak Raphanus sativus 8 36 Yam Pan/jhum alu* na 4 Sweet pumpkin Misti kumra shak Cucurbita maxima 5 37 Yam (Elephant) Ole kachu na 42 5 Thankuni Thankuni Pata Centella asiatica 12 38 Plantain Stem Kolar thore na 11 6 Bitter gourd Karala pata* Momordica charantia 18 39 Olekopi Olekopi na 34 7 Rashun Leaves Rashun shak na 5 FRUITS 6 8 Dheki leaves Dheki shak na 39 40 Pamelo (Red) Jambura (Lal) na 9 Jarul Khambang na 13 41 Papaya (ripe) Paka pepey Carica papaya 9 10 Dumurshomi Leaves Dumurshumi shak na 7 42 Pineapple (wild ) Anarash (bonno) na 6 11 Seneya Leaves Seneha shak na 13 43 Wild Melon Sindera* Cumis melo 35 12 Lelom Leaves Lelom shak na 23 44 na Roshko* Syzygium balsameum 40 13 na Sabarang* Ajuga macrosperma 33 45 Bead tree kusumgulu* Elaeocarpus angustifolius 55 14 Roselle Amila pata* Hibiscus sabdariffa 32 FISH, MEAT AND EGG 15 na Lalam pata* Premna obtusifolia 30 46 Ilsha (salted) Ilish mach Tenualosa ilisha 6 16 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa 38 47 Kachki Fish Kachki mach Corica soborna 11 17 na Ojan shak* Spilanthes calva 48 48 Poa fish Poa mach Glassogobius giuris 29 18 na Ghanda batali* Paederia foetida 54 49 Lota Fish Lota mach na 10 19 na Orai balai Premna esculenta 28 50 Churi Fish (Dried) Churi mach na 38 20 Purslane Bat slai* Portulaca oleracea 32 51 Prawns whole (dried) Chingri shampurna Heterocarpus ensifer 14 21 Yellow saraca Maytraba Saraca thaipingensis 26 52 Nappi paste Nappi na 56 22 Yellow Flower Holud fool na 9 53 Zhinuk Shell Mollusk shell 7 23 Ginger Flower Ada shak na 5 54 Crabs Kakra Liocarcinus vernalis 24 24 Sime Flower Sime fool na 13 55 Shark Hangar Carcharhinus amblyrhynchos 13 NON -LEAFY VEGETABLES 56 Shark (Dried) Hangar shutki Carcharhinus amblyrhynchos 21 25 Pea eggplant Mistti begun* Solanum spinosa 31 57 Kuchia fish Kuchia Monopterus cuchia 20 26 Solanum Tak begun* Solanum virginianum 35 58 Snails (Small) Shamuk (choto) Helix pomatia 39 27 Sigon data Sigon data* Lasia spinosa 40 59 Snails (Large) Shamuk (Boro) Helix pomati 8 28 Tara (Like Kochu data) Tara data na 19 60 Rat Idur Rattus norvegicus) 6 29 Basher Korol Basher korol na 39 61 Frog Beng Litoria caerulea 33 30 na Banchalta* na 50 62 Egg Dim Gallus bankiva 13 31 na Fakong na 48 63 na Gobar poka na na 32 Wild mushroom Edur kan na na 64 Pork Shukurer mangsha Sus scrofa domestica 54 *ethnic food **raw na: not vailable

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2.2. Analysis of nutrients in key foods

In generation of nutrient data for food composition database, designing and executing

sampling protocol, preparation of analytical samples and portions, selection of analytical

method, execution of analytical procedures with appropriate number of analytes and

analytical replicates, involvement of skilled lab personnel, evaluation of analytical values

and documentation of data are of utmost important (Greenfield and Southgate, 2003b).

Lapse in any of the process would result in error in the representative nutrient data. The

basic principles of producing quality data should give attention on-

• the collection and preparation of food sample

• the selection of the analytical method and its validation within the

laboratory carrying out the analysis of a particular food

• proper execution of methods, and

• review of the values obtained.

Therefore, adequate and appropriate care and precaution were taken in designing and

addressing these approaches.

2.2.1. Food sampling protocol

A sampling plan is the predetermined procedure for selection, collection, preservation,

transportation and preparation of the analytical portion to be used from a lot as samples.

A sampling plan should be a well organized document for program objectives (Proctor et

al, 2003).

Foods are biological materials and exhibit variation in composition, particularly prone to

variation in water, carbohydrate and vitamin contents. This variation is related to a

number of factors such as cultivation place (cultivated, wild, garden), geographical

location, seasons, state of maturity, cultivar and breed, etc. Therefore, collection of food

sample needs to be specific in terms of timing and frequency to reflect these variations.

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Food sampling is one of the most important aspects for compositional analysis. It

determines the analytical data quality that needs to provide representative nutrient value

to the users. However, food sampling is a difficult because of its variability and

heterogeneity in composition. The primary objective in food sampling is to collect a

representative food sample and to ensure that changes in nutrient composition do not

occur between collection and analysis (Greenfield and Southgate, 2003c).

Sampling error arises with using a part of total food sample. It is because of

heterogeneity nature of foods. Taking small portions at the primary sampling stage can

lead to sampling error. Sampling error is also associated with poor labeling &

documentation, non-conforming sample use, incorrect mixing, and also inappropriate

storage. In practice, 100-500g represents a convenient sample size. The larger sample

size the more reliable the sampling; however, sample size is limited by time, cost,

sampling methods and logistics of sample handling, analysis and data processing.

Therefore, replicate samples of representative amount must always be taken when

estimating the composition of food.

It is further noted that food samples should be representative of the food “as

consumed”, and as “available for consumption”. Since the database is for mass people

consumption, food samples were collected from the points from where mass people

take it for their consumption (Greenfield and Southgate, 2003b).

To minimize the geographical variation, food samples were collected from wholesale

markets located at four the entry points (figure 2.4) to Dhaka city, where consumable

matured food items come from all over the country for mass people consumption. Two

samples were collected from cultivation fields. Also to avoid sampling error, a large

portion (approximately 2.0kg) of replicate samples for every item was collected from

each collection point. Since there is limited scope to study the seasonal variation in

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nutrient composion in the food composition database, food items, particularly the

vegetables, fruits and fishes were collected during their peak available period.

2.2.1.1 General food sampling protocol

In this study a multi-regions sampling plan was used to collect representative food

samples. The identified and selected key food items were collected from four different

wholesale markets located at the four entry points to Dhaka city, and from two

cultivation fields (figure 2.4). Every two samples were pooled together to make a single

analyte (test sample), thus made three analytes for each food item, which were then

analyzed for their nutrient profile (figure 2.4). Sampling of general food item was started

at June 2009, particularly cereals and it was continued upto March, 2010.

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Figure 2.4: Multi-regions sampling plan for general food sample

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2.2.1.2 Ethnic food sampling

Ethnic food items were collected from local weekly markets at Rangamati and

Khagrachari. Three food samples for each food item were collected from each market.

Every two food samples were pooled together to make three analytes (test sample),

which were analyzed for their nutrient profile. The ethnic food sampling plan is depicted

in figure 2.5. A few ethnic foods were collected during September through December,

2009, but most of the ethnic foods were collected during April-May, 2010.

A C Analyte-I

Rangamati Bnorupa bazar

Sample A Sample B Sample C Sample F Sample E Sample D

D F Analyte-I B E

Analyte-I

Khargrachari Bazar

Figure 2.5: Multi -regions sampling plan for ethnic food

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2.2.2 Procedure for food sample collection

Representative food samples were collected from the selected wholesale markets

where food items come from all over the country and from cultivation fields. Attempt was

taken to collect tender fresh sample. Collection was made in new clean plastic poly

bags. In case of field sample collection, some water was sprayed on the vegetable

samples during packing into the poly bag, and thus kept it moistened during

transportation from the field to the lab.

For collection of general food items, particularly the vegetable items, replicate samples

of approximately 2.0kg of each food was purchased from each of the four selected

wholesale markets and from the two cultivation fields. These replicate samples were

mixed together to make a single sample for each collection point, and thus made six

samples for six collection sites. Two samples were then pooled to make a single analyte

and thus made three analytes for each food item.

Ethnic food samples were collected from weekly wholesale markets at Rangamati and

Khagrachari. Three samples for each food items of approximately 1.5kg were purchased

from each market. The samples were water sprayed and packed into new clean plastic

poly bags for transportation to the lab.

2.2.3 Identification of collected food samples

Nutrient profile in food composition database needs to be representative of the foods-

“what the mass people consume” and “from where they collect it”? To minimize the

compositional variations that may arised by geographical locations, timing of collection,

sample preparation; the food samples, particularly vegetables, fruits and fishes, were

collected from the wholesale markets where the foods arrive from four geographical

regions of the country. It thus ensured the representative consumable food items of all

geographical locations. Samples were collected at very early morning from the collection

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points, taken to the lab and immediately processed for analyte preparation at adequate

required lab environment with trained and skilled lab personnel.

Rice, maize and lentil

A market survey was conducted in different wholesale and local rice markets in and

around the Dhaka City to find out the rice varieties which were consumed by the

majority of population. It was then identified and certified by an expert at the Grain

Quality and Nutrition Division, Bangladesh Rice Research Institute, Gazipur. It was the

BRRI -29 variety. The lentil deshi and maize deshi varieties were also indentified and

certified by BRRI.

Vegetables and fruits

The vegetable and fruit items were categorically identified and certified by personnel of

Department of Agricultural Extension (DAE) and the taxonomist of the Department of

Botany, Dhaka University. In case of ethnic foods, food samples were purchased from

the weekly wholesale markets with the help of local ethnic DAE staff, who confirmed its

identity. After taking the food sample to the lab, the taxonomic expert further identified it

for its scientific and English name.

Fish, meat and eggs

The identified fresh fish samples were purchased from wholesale and local markets at

Dhaka city. Meat and egg samples were also purchased from the local market. They

were then rapidly processed for estimation of moisture content. The dried samples were

used for analysis of proximate nutrients and mineral contents.

2.2.4 Sample preparation for analysis

Generation of nutrient values employs a range of analytical procedures and it requires a

number of analytical sample portions. Taking of analytical portions and size depend on

the analytical method to be used. When food samples are used for analysis of a range

of nutrients, it is convenient to store some analytical portions (at least 3 portions) at -40

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or -70oC (Greenfield and Southgate, 2003c). Care should be taken to separate the

edible portion and inedible portion. When analytical portions are taken repetitively from

stored samples for analysis of different nutrients, it is convenient to store multiple

identical sample units in the freeze.

In this study, the properly collected food items were first rinsed with tape water followed

by washing with distilled water, then gently swabbed with tissue paper and air dried. The

cleaned air-dried sample was diced or cut into small pieces (peeled where needed)

using a cleaned stainless knife on a cleaned plastic cutting surface. Hand gloves were

used throughout the process. The diced food sample was taken to a stainless steel bowl

and mixed with a plastic spatula. Adequate precautions were taken to avoid any metal

contamination. In case of vitamin analysis, these operations were performed very fast in

dim light to avoid any degradation by oxygen and light, and for some food items,

portions of fresh process sample(s) were kept frozen. Where required, the clean air

dried sample was homogenated with a lab blender, and the required portion of the

sample analyte was taken from the homogenated material.

Vegetable and fruit analytical sampling

The vegetables and fruits were subjected to multiple nutrient analyses. Accordingly, they

were processed for analytical samplings and stored in multiple portions as –

(a) 3x5g taken for carotenoid analysis, (b) 3x5g taken for vitamin C analysis, (c) 3x20g for B-

vitamins analysis, (d) 3x10g for sugar analysis (for fruits), (d) 3x10g for dietary fiber analysis, (e)

3x10g for crude fiber analysis, (f) 3x10g taken for nitrogen analysis, (g) 3x10g taken for mineral

analysis, (h) 3x25g taken for moisture analysis, and (i) remaining portion in multiple units frozen

and stored at -20oC & -40oC depending on nutrient to be analysed.

Fish, meat and egg analytical sampling

Fish: Approximately 1.0-1.5kg fish of consuming size of each variety was collected from

3 wholesale and from 3 local markets located at Dhaka and its peripheries from where

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people purchased fish for their consumption. The fish samples were brought to lab

quickly to avoid any spoilage during transport. Ice box was used during collection of fish

from the peripheral points. Taking the sample in lab, it was cleaned and processed for

edible portion. Small fish was taken as a whole.

Meat: About 20-25 meat cuts at least 2-3 pieces from each of five regions of the

slaughted animal were purchased from butcher shop at two markets and brought in

clean plastic poly bags to the lab, where it was processed for analytical sampling.

Egg: Twelve eggs of each variety were collected from the local markets from where

mass people taken for their consumption. Each 4 eggs were pooled together to process

to make a single analyte, and thus prepared three analytes for each variety.

2.2.5 Chemicals

All chemicals and reagents used in the analysis of the nutrient profile were of analytical

grade and were purchased from Merck (Darmstadt, Germany, BDH (UK), Sigma

Chemical Co (St. Louis, MO, USA). Ascorbic acid, β-carotene, and B-vitamins, were

procured from Sigma Chemical Co. (St. Louis, MO, USA).

2.2.6 Methods of nutrient analysis

Use of appropriate and accurate methods employing skilled analysts can only ensure

reliable data for preparation of a food composition database. However, the choice of

analytical methods is limited to equipment facilities and technical staffs available.

The original project proposition was aimed to analyse 50 food items for their nutrient

profile comprising proximate composition, minerals, vitamin C, total carotenoids,

carotene profile and B-vitamins. Because of the fund constraint and time limitation,

arising out of the inclusion of additional 25 food items in the analysis, the number of

nutrients to be analyzed was reduced to proximate nutrients, minerals, vitamin C and

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carotenoid for the 75 food items. Analysis of β-carotene was limited to 20 vegetables

and fruits of both general and ethnic origin. The nutrients analysed and the analytical

techniques employed are summarized in the table 2.7.

Table 2.7: Nutrients analysed and the analytical techniqu es employed

Nutrient class Nutrients AOAC and Standards methods

Macronutrients Moisture Drying in Air oven at 100-105oC (AOAC, 1998a)

Protein Micro-Kjeldahl method (AOAC, 1998b)

Fat Soxhlet extraction (Raghuramulu et al, 2003a)

Fatty acids By calculation (Greenfield & Southgate, 2003)

Crude fiber Gravimetric (Raghuramulu et al, 2003b)

Ash Muffle furnace (AOAC, 199c)

Dietary Fiber Sigma Kit (AOAC, 1998d; Sigma TDF-100A)

Carbohydrate By Calculation (Rand et al, 1991)

Micronutrients

Vitamin Carotenoids Spectrophotometry (Roriguez-Amaya and Kimura, 2004;

Rahman et al, 1990)

β-carotene HPLC (Roriguez-Amaya and Kimura, 2004)

Vitamin C Spectrophotometry (AOAC, 1998e)

Mineral Cu, Zn, Fe, Mn, Ca,

Mg, Na, K, P

Atomic Absorption Spectrophotometry (Petersen, 2002)

Antinutrients Phytate Spectrophotometry (Wheefer and Ferral, 1971)

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2.2.6.1 Analysis of moisture

Moisture content is one of the most variable components, particularly in the plant foods.

This variability affects the food composition as a whole. Therefore, the moisture value

remains as an essential component in food composition database.

The moisture content in the food items was determined by measuring the amount of

water removed from the food (AOAC, 1998a). It was done by direct heating the food in

an Air oven at 100-105oC to constant weight.

2.2.6.2 Estimation of protein

Protein content in the food items was determined by indirect method estimating total

nitrogen in the food. It was calculated by multiplying the total nitrogen using the

respective factor as estimated by Micro-Kjeldahl method (AOAC, 1998b).

2.2.6.3 Estimation of total fat and fatty acids

The most frequently used method for fat estimation in food is the continuous extraction

of fat with petroleum ether or diethyl ether. For some specific foods, mixture of

chloroform and methanol is also used to extract fat.

In this study, dried food was subjected to continuous extraction with petroleum ether in a

Soxhlet extractor (AOAC, 1998c). Chloroform-methanol extraction was also used in

isolation of fat in some particular food items such as meat and eggs (Raghuramulu et al,

2003).

Total fatty acid content in the foods was estimated by calculation and by multiplication of

total fat content by a factor (Greenfield and Southgate, 2003d).

2.2.6.4 Estimation of ash content

In ash estimation, dried food sample is ignited at 600oC to burn out all organic materials.

The inorganic material which is ignited at this temperature is the ash.

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In this study, ash in the food sample was estimated by heating the dried sample in a

Muffle furnace at 600oC for 3h (AOAC, 1998d). Ash content was calculated from weight

difference.

2.2.6.5 Analysis of crude fibre and dietary fibre

Crude fibre was estimated by gravimetric method as described by Raghuramulu et al

(2003). The dried and fat free food sample was treated with boiling sulphuric acid at

constant volume, cooled, filtered, washed with hot water, made alkaline, boiled, filtered

and washed with water followed by ethanol and ether wash. The residue was then

heated in a Muffle furnace at 600oC for 3h. Crude fibre was finally calculated from the

weight difference.

Dietary fibre was analysed by AOAC method (1998d) using total dietary fibre assay kit

(TDF-100, Sigma Chemical Co., Saint Louis, Missouri, USA). In this method, a

combination of enzymatic and gravimetric techniques was used. Dried fat free sample

was gelatinized with heat stable α-amylase, then enzymatically digested with protease

and amyl glycosidase to remove the protein and starch present in the food sample.

Ethanol was added to precipitate the soluble dietary fibre. The residue was filtered and

washed with ethanol and acetone. After drying, half of the residue was analysed for

protein and half for ash. Total dietary fibre was the weight of the residue minus the

weight of the protein and ash.

2.2.6.6 Analysis of phytatic acid

Phytic acid was determined by spectrophotometric method (Wheeler and Ferrat, 1971).

Phytic acid in the food sample reacting with ferric chloride developed red colour with

potassium thiocyanate. This colour difference was read in the spectrophotometer at

485nm against the water blank. Intensity of the colour is proportional to ferric ion

concentration, which was used in the calculation of phytic acid content in the food

sample.

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2.2.6.7 Calculation of carbohydrate and energy

The content of available carbohydrate in the food sample was determined by difference.

Carbohydrate was calculated by subtracting the sum percentage of moisture, protein,

fat, ash, crude and dietary fibre (Rand et al, 1991; FAO, 2003).

The energy content in the food sample was calculated by the sum of protein, fat and

carbohydrate using respective Atwater factors (Rand et al, 1991).

2.2.6.8 Analysis of vitamin C

Ascorbic acid in food sample was estimated by spectrophotometric method (AOAC,

1998e). The fresh food sample (vegetable or fruit) was homogenized in a mortar with

pestle using metaphosphoric acid, filtered, treated, and incubated at 60oC for 60 minutes

with 2, 4-dinitrophenyl hydrazine. Adding 85% sulphuric acid, it was read at 520nm in

spectrophotometer (UV-1601, UV-Visible, Shimadzu).

2.2.6.9 Analysis of carotenoids

Carotenoid content in the vegetable or fruit sample was determined by acetone-

petroleum-ether extraction followed by spectrophotometric measurement (Roriguez-

Amaya and Kimura, 2004). Extraction of carotenoid was performed by grinding of

processed food sample in mortar and pestle, filtration through sintered glass filter under

vacuum and separation from acetone to petroleum ether.

When the color of the eluent is orange like, it was read at 450nm in a spectrophotometer

(UV-1601, UV-Visible, Shimadzu) for concentration of total carotenoids; when it was

green color containing chlorophyll, the extract was passed through a column packed with

activated 1:1 alumina and sodium anhydrous to remove the green pigments. The column

eluent was then read at 450nm. All preparative and extractive procedures were

performed in dim light to avaoid phoyosensitive damage.

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2.2.6.10 Analysis of β-carotene

Reverse phase HPLC (Shimadzu PC based Binary Gradient HPLC Prominence System

with PDA Detector, SPD-M20A; Solvent delivery System, LC-20AT; LC Solution Multi

Workstation Software) was used to determine the β-carotene (Roriguez-Amaya and

Kimura, 2004). The nitrogen dried carotenoid was reconstituted with mobile phase

(acetonitrile: methanol: 2-propanol-) and 50µl reconstituted sample was injected into the

VYDAC reverse phase C18 column (5µm particle size). The column was re-equilibrated

with the mobile phase for at least five minutes before the next injection. β-carotene was

purchased from Sigma Chemical Co. USA and was used as standard analytes.

2.2.6.11 Analysis of mineral profile

Mineral content in the food sample was analysed by Atomic absorption

spectrophotometric method (Petersen, 2002). Dried food sample was subjected to wet

digestion with nitric acid and perchloric acid in an auto- digestor at 325oC. The digested

sample after appropriate dilution was aspirated into the spectrophotometer where it was

burned into atomic components and it was read at their respective wavelength.

Sigma standard elements were used as standard analytes.

2.2.7 Quality assurance programme (QAP)

Method standardization and validation were carried out with internal standard (IS),

external standard (ES), intra and inter lab analysis of particular food and percent

recovery. Data quality was maintained by precision (co-efficient of variance, CV),

accuracy (Standard Reference material, SRM) and well documented foods, standard

error of mean (SEM).

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Chapter 3

Results and Discussion

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3 Results and Discussions

Healthy well-nourished people are the outcome of successful social and economic

development and constitute an essential input into the development process. Good

health needs balanced diets, which could be obtained and designed from nutrient

composition of key foods. Therefore, nutrient composition of key foods is to be well-

known and available to the mass population.

Public health nutrition activities, nutrition, agricultural, health and epidemiological

research, food industries and trade decision and government policy planning concerning

nutrition and agriculture, all depend on an accurate knowledge of what is in food. It is the

nutrient composition of food that can provide this information. Currently these data are

not adequate to meet the existing needs of planners, practioners, and professionals in

Bangladesh. Often the data are incomplete, inconsistent and inaccessible.

There is a worldwide call for updating food composition databases. The third world

countries are far behind to address this attempt. Like most of the developing countries,

Bangladesh does not have food composition database. The current food composition

table (FCT) - ‘Deshio Khadder Pustiman’ prepared by the Institute of Nutrition and Food

Science (INFS), University of Dhaka, later edited by Helen Keller International (HKI) in

english version- ‘Tables of Nutrient Composition of Bangladeshi Foods’ was prepared

long back; most of the nutrient data used were analyzed long ago, and some were

assumed to be borrowed from neighboring countries, and did not have the nutrient data

of ethnic foods.

Over the last decade food composition activities have increasingly been addressed by

many agencies. As an effort to contribute to this need, this study has been undertaken

with an aim to prepare a food composition database with reference to general and ethnic

foods of Bangladesh.

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3.1 Key food identification

Nutrient profiling of a food is expensive in term of its identification, collection, processing

and analysis. Therefore, analyzing every food item for every nutrient and meeting all

user requirements is difficult. Consequently, priorities must be determined. Key foods

have been identified as those foods that contribute up to 75% of any one nutrient to the

dietary intake (Haytowitz et al, 1996; 2000; 2002). Key foods can be documented by

food consumption survey.

In this study, the key foods was indentified through CFCS and FGDs and priorities made

in consultation with TAT members.

3.1.1 Comprehensive Food Consumption Survey (CFCS)

Food consumption survey comprises collection of information about food intake

frequency and amount of food consumed (Brussaard et al, 2002). It is performed by

household survey. The aim of CFCS is to generate food consumption statistics. Food

consumption data and nutrient values help to generate Key Foods list. In identifying the

key foods, nutrient contribution of the food and public health significance of nutrients are

taken into consideration.

The proposal was to conduct CFCS on 1700 households comprising 1200 general

households and 500 ethnic tribal households. Later on as per recommendation received

from the 5th dissemination workshop at Rangamati on the 18th March 2010, more ethnic

households were included in the CFCS to make a total 805 ethnic households

CFCS activities

To select the key food items to be investigated for their nutrient profiling, CFCS was

carried out to collect food consumption data of the general and ethnic tribal population.

Before starting it, survey locations were mapped out, a questionnaire was developed

and pretested and sample size was determined. These activities were finalized and

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approved in consultation with FAO Technical Advisory Team members. CFCS sampling

plan of the general and ethnic households are described in the table 3.1 and 3.2, and

some CFCS activities in ethnic tribes are highlighted in the photographs.

CFCS Team

Co-Investigator and DAE enumerator with ethnic peo ple

Enumerator taking interview

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Enumerator with ethnics

PI and enumerator with ethnics taking interview

Co- Investigator and enumerator with ethnics taking interview

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Table 3.1: Location and description of CFCS data collection among the general population

Division District Upazilla Date of visit Location No of HH interviewed

Type of HH

Dhaka Netrokona Netrokona sadar

31.01.09 to 02.02.09 West chakpara 50 Urban Mohandrapur 54 Rural

Manikgonj Saturia 08.02.09 to 11.02.09 Sawdagar para & Uttarkaunna

50 Urban

12.02.09 to 16.02.09 Char saturia 50 Rural Sylhet Moulavibazar Moulavibazar

Sadar 21.02.09 to 22.02.09 Suvro 52 Urban 23.02.09 to 25.02.09 Kodupur 50 Rural

Habigonj Madhobpur 25.02.09 to 27.02.09 Godampara & Krishnanagar

51 Urban

28.02.09 to 02.03.09 West madhobpur 50 Rural Chittagong Feni Feni Sadar 16.03.09 to 17.03.09 North Charipur 50 Urban

18.03.09 to 19.03.09 Nagarkandi, Mathiara 50 Rural Comilla Comilla Sadar 20.03.09 to 21.03.09 Gabindapur 50 Urban

22.03.09 to 24.03.09 Kashinathpur 50 Rural Rajshahi Natore Natore Sadar 03.04.09 to 04.04.09 Uttar Patua para 51 Urban

Ulupur 49 Rural Rajshahi Rajpara 01.04.09 to 02.04.09 Terkhadia 52 Urban

Kashia danga 52 Rural Khulna Jessore Jessore

Kotoali 04.04.09 to 06.04.09 Shangkarpur 50 Urban

Mubarak Kathi 49 Rural Jhenaidah Kaligonj 08.04.09 to 09.04.09 Arpara Nadir par 50 Urban

Mithapukur 50 Rural Barisal Barisal Barisal Kotoali 11.04.09 to 14.04.09 Ganopara 50 Rural

Rupatoli 50 Urban Jhalokathi Jhalokathi

Sadar 13.04.09 to 14.04.09 Krishnakathi 50 Urban

Rajapremhar 50 Rural Total 1210

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Table 3.2: Location and description of CFCS data co llection among the ethnic population

Division District Upazilla Time of visit Location No of HH interviewed

Type of

household

Dhaka Netrokona Durgapur 03.02.09 to 07.02.09 Gopalpur, Nolua 28 Hajong

Debdul 31 Garo

Sylhet Moulavi Bazar Kamolgonj 18.02.09 to 20.02.09 Tilokpur 25 Monipuri

Magurchara & Kashiapunji 25 Khasia

Chittagong Khagrachari Khagrachari sadar

31.03.10 to 15.04.10

17.04.10 to 23.04.10

Nilkantipara 70 Marma

Dewanpara 60 Chakma

Soyanundarpara 50 Tripura

Rangamati Rangamati sadar

03.04.10 to 10.04.10 Haja Chara, Diglibak, Shap Chari,

69 Chakma

Naraichari, Vhulu Chari, 21 Tanchanga

Tanchanga para, Banna Chari 30 Marma

Bandarban Bandarban sadar

06.03.09 to 07.03.09 Raicha Senior para 30 Tanchanga

07.03.09 to 08.03.09 Kalaghata 37 Tripura

09.03.09 to 12.03.09 Balaghata Biddopara, Painchara, Parjatan Chakma para, Pain para, Nadir par, Balaghata bazar

109 Chakma

13.03.09 to 14.03.09 Bameri para 30 Murang

Faruk para 30 Bam

Puratan and nutun choroi para 71 Marma

Rajshahi Rajshahi Godagari 29.03.09 to 31.03.09 Nimghat para, Nobai bottala, Dangapara, Nimghatu para

89 Shaotal

Total 805

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Figure 3.1: Distribution of general and ethnic households by number

1210

805

General Ethnic

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Figure 3.2: Distribution of selected general househ olds by division and household type

1

21

41

61

81

101

104 100 100 101 99 100

100 103 100 103 100 100

Rural Urban

Number of

households

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Figure 3.3: Distribution of ethnic households by di strict

0

50

100

150

200

250

300

350

59 50

180

120

307

89

Number of

households

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Figure 3.4: Distribution of ethnic households by tr ibe and number

238

17151

8730

30

25

2589

31

28

Chakma Marma Tanchanga Tripura

Bam Murong Monipuri Khashia

Shantal Garo Hajong

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3.1.2 Focus group discussions (FGDs)

In the present study, FGDs were conducted to enrich and supplement the CFCS food

consumption data. It carried out among the ethnic community of Marma, Chakma,

Tripura and Tangchaga tribes living in Khagrachari and Rangamati. It was done during

March and April, 2010. The FGD composed of 8-12 community participants, 2 key

informants- one from the community NGO person and one was DAE block supervisor,

and a critique- the agriculture officer. The composition, characteristics and activities of

the FGDs are depicted in the table 3.3 and photographs.

The key question was the type of foods that the ethnic people consume throughout the

year. Their response to this issue was discussed, criticized and recorded carefully. In

the CFCS it is indicated that ethnic people consumed about 46 food items, most of

which are also consumed by the general people; therefore, these are not absolutely

ethnic. To explore the true ethnic foods, the FGDs were conducted among the ethnic

communities. FGDs showed that aboutt 47 foods comprising leafy vegetables, non-

leafy vegetables, fruits, fish and meat of wild origin are consumed by the ethnic

people. The outcome of the FGDs is listed in the table 3.4.

Table 3.3: FGDs settings

FGD community

Objective Location No. of participant s

Duration of discussion

Marma Type of food intake throughout the year

Pankhaiya para, Khagrachari, CHT 12 90 minutes

Chakma South Rangapani, Bidhadhan Chakma Bari, Chakma palli, Rangamati, CHT

8 60 minutes

Tanchanga Tanchaga para, Dharmaraj Babu Bari, Kotoali, Rangamati, CHT

8 75 minutes

Tripura Ghasbhan no 2 project Gram, Jagonnath Mandir, Khagrachari, CHT

10 90 minutes

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Table 3.4: FGD outcome: Food consumption pattern of the Marma, Chakma, Tanchanga and Tripura communities

na: not available * ethnic food

Sl no English name Bengali name Scientific name Sl no English name Bengali name Scientific name LEAFY VEGETABLE S 25 na Banchalta* na 1 Rashun Leaves Rashun shak na 26 na Fakong na 2 Dheki leaves Dheki shak na 27 na Hahnagulu na 3 Jarul Khambang na 28 Yam Pan/jhum alu* na 4 Dumurshomi Leaves Dumurshumi shak na FRUITS 5 Seneya Leaves Seneha shak na 29 Pamelo (red) Jambura (Lal) na 6 Lelom Leaves Lelom shak na 30 Pineapple (wild ) Anarash (bonno) na 7 na Sabarang* Ajuga macrosperma 31 Wild Melon Sindera* Cumis melo 8 Roselle Amila pata* Hibiscus sabdariffa 32 na Roshko* Syzygium balsameum 9 na Lalam pata* Premna obtusifolia 33 Bead tree kusumgulu* Elaeocarpus angustifolius 10 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa FISH AND MEAT 11 na Ojan shak* Spilanthes calva 34 Lota Fish Lota mach Na 12 na Ghanda batali* Paederia foetida 35 Churi Fish (Dried) Churi mach na 13 na Orai balai Premna esculenta 36 Nappi paste Nappi na 14 Purslane Bat slai* Portulaca oleracea 37 Zhinuk Shell Mollusk shell 15 Yellow saraca Maytraba Saraca thaipingensis 38 Crabs Kakra Liocarcinus vernalis 16 Yellow Flower Holud fool na 39 Shark Hangar Carcharhinus amblyrhynchos

17 Ginger Flower Ada shak na 40 Shark (dried) Hangar shutki Carcharhinus amblyrhynchos

18 Sime Flower Sime fool na 41 Kuchia fish Kuchia Monopterus cuchia NON –LEAFY VEGETABLES 42 Snails (small) Shamuk (choto) Helix pomatia

19 Pea eggplant Mistti begun* Solanum spinosa 43 Snails (large) Shamuk (Boro) Helix pomati 20 Solanum Tak begun* Solanum virginianum 44 Rat Idur Rattus norvegicus) 21 Sigon data Sigon data* Lasia spinosa 45 Frog Beng Litoria caerulea 22 Tara (Like Kochu data) Tara data na 46 na Gobar poka na 23 Basher Korol Basher korol na 47 Pork Shukurer mangsha Sus scrofa domestica 24 Wild mushroom Edur kan na

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FGDs activities

FGD in Marma community in Marma palli, Khagrachari

FGD in Marma community in Marma palli, Khagrachari

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64

FGD in Chakma community in Chakma palli, Rangamati

FGD in Chakma community in Chakma palli, Rangamati

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65

FGD in Tanchanga community in tanchanga palli in Ra ngamati

FGD in Tanchanga community in tanchanga palli in Ra ngamati

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66

FGD in Tripura community in Tripura palli, Khagrach ari

FGD in Tripura community in Tripura palli, Khagrach ari

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67

3.1.3 Lifestyle characteristics of general and ethnic people

The key objective of the CFCS was to obtain food consumption information of the

general and ethnic population of Bangladesh. In addition to collecting the food

consumption data, the lifestyle profile of this population was also addressed.

Analysis of socioeconomic data showed that amongst the 1210 general households

(table 3.1) only 65 households were female headed and the rest were the male headed.

The male-headed urban household heads were more educated in numbers than their

counter part in rural locations (tables 3.5). Their main occupation was found to be earth

cutting. It may be because of their low educational level as well as currently running

road and civil works in the rural and semi urban areas. Female headed household

heads were mostly engaged in household works. Mean age of the male headed

household heads were similar in rural and urban areas. Female headed household

heads were comparatively older than the male head. The monthly income and

expenditure of both the urban and rural households were found similar.

Prevalence of illiteracy was high among the Marma and Shaontal while Chakma and

Tripura were more educated, and consequently Chakma and Tripura people were

employed in services (table 3.8). The monthly family income was found to be highest

among the Tripura followed by Chakma, and lowest income was found in the Marma

and Shaontal tribes.

Food security data indicated that almost 3% households frequently experienced food

shortage, while 12% percent reported to have food shortage infrequently (tables 3.6,

3.9). Food insecurity was high in February of the year. Rural (46%) and Urban (54%)

households reported to have infrequent balanced diet. Almost 9% household ate less

than three times a day. In food shortage, adult women had to eat less and it was higher

among the rural than the urban households. Compared to the general population, food

insecurity was high among the ethnic people. It was found higher among Marma and

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68

Shaontal while food security was comparatively better among the Tripura, Tanchanga

and Chakma tribes.

In term of morbidity, most of the rural and urban household heads reported the suffering

of their under five children from diarrhea in the last one month (tables 3.7, 3.10). Most of

them did not take any specific care for the treatment of diarrhea. Comparing the

prevalence of diarrhea among the general population, prevalence of diarrhoea among

the ethnic under five children was found too high. It was found to be lowest among the

Tanchanga and highest among the Tripura children.

The lifestyle data reveal that the ethnic people are far behind the general population in

terms of socioeconomic situation, food security and health care access facilities.

Special care should be taken to address these problems.

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69

Table 3.5: Socioeconomic profile of general households

Parameters Urban Rural

Frequency Percent Frequency Percent

Type of household (HH) 606 50.1 604 49.9

Gender of household head Male Female

575 31

94.9 5.1

570 34

94.4 5.6

Education of male headed HH head Below primary Below SSC Below HSC HSC to Below BSc BSc to MSc Illiterate can sign only Can read and sign Total

Education of female headed HH head Below primary Below SSC Below HSC Illiterate can sign only Can read and sign Total

106 162 37 39 4

61 75 121 606

1 6 3 3

12 6

31

17.5 26.8 6.1 6.5 0.7

10.1 12.30 20.0 100.0

3.4

17.2 10.3 10.1 39.0 20.0 100.0

129 156 34 32 2

222 28 -

604

7 1 0 12 9 5 34

21.3 25.9 5.7 5.3 0.4 36.7 4.7 -

100.0

20.0 3.3 -

36.7 25.0 15.0

100.0

Occupation of male headed HH head Agri (work) Earth cutting Rickshaw / van driver Others Total

20 574

2 10 606

3.3 94.7 0.4 1.6

100.0

13 583

8 -

604

2.1 96.5 1.4 -

100.0

Occupation of female headed HH head Agri (work) Earth cutting Household work NGO worker Others Total

1 6

18 4 2

31

3.4 17.2 58.6 13.9 6.9

100.0

- 2 30 20 -

34

-

6.7 86.6 6.7 -

100.0 Mean ± Sd Percent Mean ± Sd Percent

Age of male headed HH head (Year) 15-30 30-45 45-60 60-75 Total

26.68±3.40 38.67±4.35 51.90±3.87 66.10±3.53 40.73±1.42

23.3 45.9 25.4 5.4

100.0

27.30 ± 2.95 38.37± 4.28 53.11 ± 4.76 67.15 ± 3.49

41.23 ± 11.64

21.3 48.9 23.9 5.9

100.0 Age of female headed HH head (Year)

15-30 30-45 45-60 60-75 Total

27.25±3.20 40.08 ±3.77 52.78 ±4.24 66.67 ±2.89 45.00 ±11.79

13.8% 44.8% 31.0% 10.3%

100.0%

26.00 ± 0.00 39.23 ± 4.02 55.00 ± 4.88 70.00 ± 0.00

48.20 ± 11.21

3.3 43.3 46.7 6.7

100.0

Monthly total income (Tk.) <5000 5001 – 8000 5001 – 8000 8000 – 11000 11000 – 14000 >14000 Total

4093.82 ± 926.68 6673.87 ± 827.44 9653.40 ± 749.35 12520.00± 699.55

18976.00 ±5949.64 7837.54 ± 4556.45

30.69 36.63 17.00 7.43 8.25 100.0

4058.38 ± 931.65 6714.47 ± 843.55 9493.94 ±745.71

12411.36 ±790.41 20924.00 ±7222.35 8006.99 ± 5075.75

28.64 39.40 16.40 7.28 8.28

100.0

Monthly average total expenditure (Tk.) 6259.28 ± 3148.04 100.0 6169.38 ± 3324.94 100.0

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70

Table 3.6: Food security by households’ type in general population

Parameters

Urban Rural

Frequency Percent Frequency Percent

Experience food shortage in family Never ever Some times Often/always Total

517 74 15 606

85.3 12.2 2.5

100.0

522 64 18 604

86.5 10.6 3.0

100.0

Time of food shortage January February Whole year Total

21 64 4

89

23.60 71.91 4.49

100.0

22 58 2

82

26.83 70.73 2.44

100.0 Status of getting balance food

Always Never ever some times Total

242 38 326 606

39.9 6.3 53.8

100.0

282 42 280 604

46.7 7.0 46.4

100.0 HH head ate < 3 times a day

Yes No Total

54 552 606

8.9 91.1

100.0

57 547 604

9.4 90.6

100.0

Children ate <3 times a day Yes No Total

25 581 606

4.1 95.9

100.0

16 588 604

1.7 97.5

100.0 Children starve d whole day

Yes No Total

12 594 606

2.0 98.0

100.0

5

598 604

1.0 99.0

100.0 Adult member starve d whole day

Yes No Total

29 577 606

4.8 95.2

100.0

19 585 604

3.1 96.9

100.0

Weight loss any member Yes No Did not verify Total

8 573 25 606

1.32 94.55 4.13

100.0

8 581 15 604

1.33 96.19 2.48

100.0

Who ate less during food s hortage None response Adult women Adult men Total

500 86 20 606

82.51 14.19 3.30

100.0

456 133 15 604

75.50 22.02 2.48

100.0

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71

Table 3.7: Morbidity and its treatment by household type in general population

Response

Urban Rural

Frequency Percent Frequency Percent

Eating adequately but not gainin g weight Yes No Don’t understand Total

23 530

53 606

3.8 87.5

8.7 100.0

19 524

61 604

3.1 86.8 10.1

100.0

Member suffers from stomach ache Yes No Don’t know Total

29

575 2

606

4.8

94.9 0.3

100.0

28

576 -

604

4.6

95.4 -

100.0 Know ledge about reasons of diarrhoea

Answered rightly Answer partly right Wrongly answered Total

446 117

43 606

73.7 19.3

7.1 100.0

397 162

45 604

65.6 26.8 7.5

100.0 Diarr hoea in any <5 children in last month

Didn’t experienced Last week One month ago More than one month ago Total

471

14 24 97

606

77.6

2.3 4.0

16.0 100.0

478

13 33 80

604

79.2 2.2 5.5

13.2 100.0

Measures taken to get rid of diarrhoea Didn’t experienced Fed home prepared saline Fed packet saline Medicine Medicine and oral saline Total

471

14 64 4

53 606

77.8

2.3 10.6

0.7 8.7

100.0

478

10 62

4 50

604

79.1 1.7

10.3 0.7 8.2

100.0 Giving anti helminthes drug regularly to <6y children

Cannot remember Yes No Total

309 245

52 606

51.0 40.5

8.6 100.0

334 223

47 604

55.3 36.9 7.8

100.0

Immuniz ation to child ren Don’t know Complete Incomplete Total

339 260

7 606

55.9 42.9

1.2 100.0

357 236

11 604

59.1 39.1 1.8

100.0

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72

Table 3.8: Socioeconomic profile of ethnic households

Parameters CHAKMA MARMA SHAONTAL TRIPURA TANCHANGA OTHERS

Type of household Frequency % Frequency % Frequency % Frequency % Frequency % Frequency % Education Level HH Head

Below primary Below SSC Below HSC HSC and above Illiterate can sign only Can read and sign Total

20 48 26 51 82 9 2

238

8.3

20.4 11.1 21.3 34.3 3.7 0.9

100.0

9 -

18 -

127 7 -

161

5.6 -

11.3 -

78.9 4.2 -

100.0

2

27 4 1

50 4 -

88

2.3 10.2 4.5 1.1 56.8 4.5 -

100.0

-

19 33 9 24 2 -

87

-

21.6 37.8 10.8 27.0 2.7 -

100.0

2 19 5 -

33 12 -

70

3.3 267 6.7 -

46.7 16.7

- 100.0

14 34 15 11 70 23 -

166

8.1 20.3 9.3 6.4 41.9 14.0

- 100.0

Occupation of HH head Agri (work) (1) Earth cutting (2) Rickshaw / van driver (5) Business (7) Jobless (9) Service (11) Others (12) Total

79 2 7

11 -

79 60 238

33.3 0.9 2.8 4.6 -

33.3 25.0 100.0

63 - 7

11 9 -

70 161

39.4

- 3.2 7.0 5.6 -

43.7 100.0

38 - 4 - 5

12 29 88

43.2

- 4.5 -

5.7 13.6 33.0

100.0

- - - 7 -

71 9 87

- - -

8.1 -

81.1 10.8 100.0

19 - 2 9 - 2 37 70

26.7

- 3.3 13.3

- 3.3 53.3

100.0

46 - 4 8 2

20 86 166

27.9

- 2.3 4.7 1.2 12.2 51.7

100.0

n Mean±sd n Mean±sd n Mean±sd n Mean±sd n Mean±sd n Mean±sd Age (y) dist ribution of HH Head

15-30 30-45 45-60 60-75 Total

31 145 53 9

238

26.5±3.01 38.3±3.82 53.1±4.57 69.5±4.20 41.2±10.50

20 63 57 20 161

28.1±2.52 38.5±3.96 53.5±4.27 66.4±3.09 46.2±12.4

14 42 28 4

88

27.6±2.41 37.2±4.27 52.8±3.50 66.0±4.24 42.0±11.1

12 49 16 9 87

27.4±4.22 39.7±3.88 51.6±3.69 66.3±2.99 43.1±11.3

14 33 19 5 70

28.2±1.94 38.4±4.07 52.8±4.98 70.0±1.09 42.3±12.1

18 78 58 13 166

27.8±2.07 38.1±4.30 52.8±4.25 66.5±4.29 44.3±11.3

Family monthly income (taka) <5000 5001 – 8000 8000 – 11000 11000 – 14000 >14000 Total

145 57 18 11 7

238

3090±949 6965±884 9375±694 12600±894 1667±2887 5306±3462

138 20 - - -

161

2780±1088 6444±846

- - -

3265±1635

82 5 1 - -

88

2343±1268 6100±548 10000.0

- -

2643±1704

24 19 24 9 12 87

3640±1418 6875±991 9500±667 12375±478

23200±11344 9510±7292

-

56 7 5 2 70

-

2739±1066 7000±1000 9500±707 14000±210 4034±2997

-

130 25 9 2

166

- 3055±1182 6301±832 9709±923

12000±304 4004±2286

Family monthly expenditure 238 6574±4392 161 3768±1754 88 3125±998 87 10512±1189 70 5345±1979 166 5478±2137

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73

Table 3.9: Food security of ethnic tribes

Parameters CHAKMA MARMA SHAONTAL TRIPURA TANCHANGA OTHERS Freq. % Freq. % Freq. % Freq. % Freq. % Freq. %

Experience food sho rtage in family Never ever Some times Often/always Total

163 64 11

238

68.5 26.9 4.6

100.0

98 61 2

161

60.6 38.0 1.4

100.0

30 36 22 88

34.1 40.9 25.0

100.0

73 14

- 87

83.8 16.2

- 100.0

51 12 7

70

73.3 16.7 10.0

100.0

113 36 17

166

68.0 21.5 10.5

100.0 Time of food shortage

January February Whole year Total

162 64 11

237

68.3 26.9 4.8

100.0

98 42 21

161

61.1 25.9 13.0

100.0

31 45 12 88

35.0 51.0 14.0

100.0

85

- 2

87

97.3

- 2.7

100.0

48 17 3

68

68.0 24.0 4.0 78

105 37 19

151

63.0 22.1 11.7 96.8

Status of getting balance food Always Never ever some times Total

95 13

130 238

39.8 5.6

54.6 100.0

41

- 120 161

25.4

- 74.6

100.0

12 3

73 88

14.0 3.0

83.0 100.0

54

- 33 87

62.2

- 37.8

100.0

26 2

42 70

36.7 3.3

60.0 100.0

44 16

105 166

26.7 9.9

63.4 100.0

HH head ate < 3 times a day Yes No Total

68

170 238

28.7 71.3

100.0

25

136 161

15.5 84.5

100.0

46 42 88

52.3 47.7

100.0

-

87 87

-

100.0 100.0

16 54 70

23.3 76.7

100.0

43

123 166

25.7 74.3

100.0 Children ate <3 times a day

Yes No Total

51

187 238

21.3 78.7

100.0

11

150 161

7.0

93.0 100.0

40 48 88

45.5 54.5

100.0

0

87 87

-

100.0 100.0

9

61 70

13.3 86.7

100.0

20

146 166

12.2 87.8

100.0 Children starved whole day

Yes No Total

2

236 238

0.9

99.1 100.0

2

159 161

1.4

98.6 100.0

7

81 88

8.0

92.0 100.0

-

87 87

-

100.0 100.0

-

70 70

-

100.0 100.0

4

162 166

2.3

97.7 100.0

Adult member starved whole day Yes No Total

13

225 238

5.6 94.4

100.0

9

152 161

5.6

94.4 100.0

13 75 88

14.8 85.2

100.0

-

87 87

-

100.0 100.0

5

66 70

6.7

93.3 100.0

14

152 166

8.2

91.8 100.0

Weight loss in any member Not respond Yes No Did not verify Total

82 2

79 75

238

34.3 0.9

33.3 31.5

100.0

36

- 70 54

161

22.5

- 43.7 33.8

100.0

21

- 30 37 88

23.9

- 34.1 42.0

100.0

31

- 56

- 87

35.1

- 64.9

- 100.0

12 2

47 9

70

16.7 3.3

66.7 13.3

100.0

19 5

111 31

166

11.6 2.9

66.9 18.6

100.0 Who ate less during food shortage

Non response Adult women Adult men Others Total

162

7 60 9

238

68.2 2.8

25.2 3.7

100.0

88 40 25 8

161

54.4 24.6 15.8 5.3

100.0

33 19 34 2

88

37.5 21.6 38.6 2.3

100.0

87

- - -

87

100.0

- - -

100.0

49 5

16 -

70

70.0 6.7

23.3 -

100.0

102 22 23 20

166

61.5 13.0 13.7 11.8

100.0

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74

Table 3.10: Morbidity and its treatment by ethnic tribes

Parameters CHAKMA MARMA SHAONTAL TRIPURA TANCHANGA OTHERS

Freq. % Freq. % Freq. % Freq. % Freq. % Freq. % Eating adequately but not gaining weight

Not respond Yes No Don’t understand Total

15 15

137 71

238

6.5 6.5 57.4 29.6

100.0

- -

93 68

161

- -

57.7 42.3

100.0

- 1 71 16 88

-

1.1 80.7 18.2

100.0

- 5 80 2 87

-

5.4 91.9 2.7

100.0

- -

65 5 70

- -

93.3 6.7

100.0

1 10

140 15

166

-

0.6 5.8 84.3 9.3

Member suffers from stomach ache Yes No Don’t know Total

9

220 9

238

3.7 92.6 3.7

100.0

2

159 -

161

1.4 98.6

- 100.0

-

88 -

88

-

100.0 -

100.0

-

87 -

87

-

100.0 -

100.0

12 54 5 70

16.7 76.7 6.7

100.0

19

146 1

166

11.6 87.8 0.6

100.0 Knowledge about reasons of diarrhea

Answered rightly Answer partly right Wrongly answered Total

134 71 33

238

56.5 29.6 13.9

100.0

68 73 20

161

42.2 45.1 12.7

100.0

32 43 12 88

36.8 49.4 13.8

100.0

87 - -

87

100.0

- -

100.0

42 12 16 70

60.0 16.7 23.3

100.0

97 52 17

166

58.1 31.4 10.5 100.0

Diarrhea in any ≤5 children in last month Didn’t experienced Last week One month ago More than one month ago Cannot remember Total

117 2 -

86 33

238

49.1 0.9 -

36.1 13.9

100.0

60 3 -

49 49

161

37.1 1.6 -

30.6 30.6

100.0

48 1 3 21 15 88

54.0 1.1 3.4 24.1 17.2

100.0

40 - 2 16 28 87

28 2 5 16 19 70

40.0 3.3 6.7 23.3 26.7

100.0

74 1 18 26 46

100

44.8 0.6 10.9 15.8 27.9

100.0

44.8 0.6 10.9 15.8 27.9 100.0

Measures taken to get relief of diarrhoea Didn’t experienced Fed packet saline Medicine Medicine and oral saline Total

152 48 13 24

238

63.9 20.4 5.6 10.2

100.0

126 12 18 6

161

78.2 7.3 10.9 3.6

100.0

63 15 3 7 88

71.3 17.2 3.4 8.0

100.0

68 9 2 7 87

78.4 10.8 2.7 8.1

100.0

37 28 2 2 70

53.3 40.0 3.3 3.3

100.0

122 19 14 11

166

73.3 11.2 8.7 6.8

100.0 Giving anti helminthics regularly to <6y children

Cannot remember Yes No Total

108 79 51

238

45.4 33.3 21.3

100.0

44 76 41

161

27.1 47.1 25.7

100.0

37 22 29 88

42.0 25.0 33.0

100.0

33 49 5 87

37.8 56.8 5.4

100.0

21 40 9 70

30.0 56.7 13.3

100.0

74 68 24

166

44.8 40.7 14.5 100.0

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75

3.1.4 Identification of Key foods

The key food approach is used to identify and select food items for analysis of nutrient

profile. It concentrates to utilize analytical resource on those foods that contribute significant

amounts of nutrients of public health significance to the diet (Haytowitz et al, 1996). It is

done by analysis of CFCS data. The purpose of key food list is to select important foods for

human nutrition and to provide nutrients of public health benefit.

In this study, CFCS and FGDs identified a total of 138 food items comprising- 54 foods

consumed by both the general and ethnic population, 20 foods consumed only by the

general and 64 foods consumed only by the ethnic people (figure 3.5). The distribution of

food groups in the common, general and ethnic foods are depicted in the figures 3.6, 3.7 and

3.8.

Figure 3.5: Number of food item (n=138) consumed by population type

64

20

54

Only Ethnic Only General Both

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76

Figure 3.6: Distribution of common food item (n =54) consumed by ≥5% HH

Figure 3.7: Distribution of ethnic food (n=64) of f ood items consumed by ≥5% HH

Figure 3.8: Distribution of general (n=20) food items consumed by ≥5% HH

117

23

16

8

Cereal Pulses Leafy Veg Non-Leafy Veg Fruits Animal

4

3

10

3

Leafy Veg Non-Leafy Animal Fruit

2

22

16

8

16

Cereal Leafy Veg Non-Leafy Veg Fruits Animal Foods

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77

3.1.5 Selection of key foods

The CFCS and FGDs identified 138 key food items. From this list, 75 food items were

selected for analysis of their nutrient profile aiming to prepare the food composition

database.

The objective of food composition database is to ensure inclusion of a range of food items

eaten by the population for which the database is being prepared. However, ideally a truly

“comprehensive database” is, in fact, an impossible objective. It is primarily because of very

large number of foods in the human diet. The volume of analytical work required for nutrient

profiling and resource implications also make it difficult. Therefore, a strategy needs to be

developed for establishing priorities in selecting food items for inclusion into the database

(Greenfield and Southgate, 2003e).

In addition to considering the nutrient input of the foods, nutrient contribution of the food to

energy intake should be focused first. Food items of public health significance also need to

be addressed. In Bangladesh, micronutrient deficiency is a public health issue; deficiencies

in vitamin A, iron, and iodine are acute problems (WFP: Micronutrient deficiencies in Bangladesh;

Country summary-Bangladesh). Zinc deficiency is widespread; highly prevalent in children in

developing countries (Zinc Nutritive Initiative: http://www.zinc-crops.org/why_zinc.html). Bangladeshi

children are also suffering from zinc deficiency (Nutrition Country Profiles: Bangladesh;

http://www.tulane.edu/~internut/ Countries/Bangladesh/bangladeshlxx.html). Bangladesh currently exports

certain vegetables. Therefore, importance of food trade also needs to be taken into account

in selection of key foods.

Food grouping is important in the selection of key foods. This ensures that the diet as a

whole is considered and that the focus is not distorted by emphasizing one food group at the

expense of the diet as a whole. Most food composition database have between 10 and 25

food groups, however, it is culturally dependent. It is to be noted that food group should

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focus food intakes by population rather than food intake by individual (Greenfield and

Southgate, 2003e).

The project proposition was to analyze 50 foods for their nutrient profile including proximate,

vitamin C, carotenoids, carotene profile, B-vitamins, fatty acids, antinutrient phytate, and

minerals. In compliance with the recommendation made at Rangamati workshop for

inclusion of more ethnic tribal foods, the food list was increased to 75 and the nutrient profile

was condensed to concomitantly in analysis of proximates, vitamin C, carotenoids, and β-

carotene, antinutrient phytate and minerals for the 75 food items.

In selecting this 75 key food, priority selection criteria were employed in which- food items

consumed by ≥15% of households were included in the key food list; the ethnic foods

consumed by >15% households but yet by a very minor group of ethnic population, were

excluded; also foods containing less micronutrient (poor health significance, such as foods

contain less or no β-carotene) were excluded. The key food list which are consumed by

both the general and ethnic, and food items consumed by only the ethnic are presented in

tables 3.11 and 3.12.

Because of the limitation to the number of food to be included, priority was given to focus the

food groups in selecting the key foods so that the selected key foods represent a whole diet.

The selected key foods included the most commonly and frequently consumed food groups

such as- cereals, lentil, vegetables, fruits, fishes, eggs and meats.

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Table 3.11: Key food list consumed by both the native general and *ethnic people

Sl no English name Bengali name Scientific name Sl no English name Bengali name Scientific name Sl

no English name Bengali name Scientific name

CEREAL 26 Yellow saraca Maytraba Saraca thaipingensis 51 Amla Amloki Emblica officinalis

1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa ROOTS & TUBER 52 Melon (mix) Melon (mix) Bangi/futi

2 Rice sunned** Atap chal Oryza sativa 27 Potato Gol Alu Solanum tuberosum 53 Wood apple Bael Aegle marmelos

3 Maize Vutta Zea mays 28 Sweet potato (red) Misti alu Ipomoea batatas 54 Palm (ripe) Paka tal Borassus flabellifer

PULSE Dal 29 Carrot Gazor Daucus carota 55 Pineapple (jaldogi) Anarosh Ananas comosus

4 Lentil (deshi) Masur dal Lens culinaris non-LEAFY VEGETABLES 56 Monkey jack* Monkey jack* Deuwa*

LEAFY VEGETABLES 30 Egg plant Begun Solanum melongena 57 Burmese grape* Burmese grape* Lotkon*

5 Joseph’s Coat Lalshak Amaranthus gangeticus 31 Bitter Gourd Karola Momordica charantia 58 Wild Melon Sindera* Cumis melo

6 Spleen Amaranth Data shak Amaranthus dubius 32 Sweet pumpkin Misti kumra Cucurbita maxima 59 Roshko* Syzygium balsameum

7 Bottle Gourd Lau shak Lagenaria siceraria 33 Kakrol Kakrol Momordica cochinchinensis 60 Bead tree kusumgulu* Elaeocarpus angustifolius

8 Radish Mula shak Raphanus sativus 34 Ladies finger Dherosh Abelmoschus esculentus FISHES

9 Coco-yam Sobuj kochu shak Colocasia esculenta 35 Green papaya Kacha papay Carica papaya 61 Carp Ruhi Labeo rohita

10 Jute Pat shak Corchorus capsularis 36 Folwal Potol Trichosanthes dioica 62 Tilapia Tilapia mach Anabus testudineus

11 Indian spinach Poi shak Basella alba 37 Green chilli Kacha marich Capsicum frutescens 63 Dragon Fish Pangash Pangasius pangasius

12 Spinach Palong sag Spinacia oleracea 38 Pea eggplant Mistti begun* Solanum spinosa 64 Sunfish Mola mach Mola mola

13 Swamp Morning-glory Kalmi shak Ipomoea aquatica 39 Solanum Tak begun* Solanum virginianum 65 Arguskala Kachki mach Scatophagus argus

14 Thankuni Thankuni Pata Centella asiatica 40 Sigon data Sigon data* Lasia spinosa 66 Taki fish Taki mach Channa puncpatus

15 Coriander Dhane pata Coriandrum sativum 41 Yam Pan/jhum alu* Dioscorea pentaphylla 67 Silver Carp Silver Carp Hypophthalmichthys nobilis

16 Spearmint Pudina pata Mentha viridis 42 Banchalta Banchalta* Dillenia pentagyna 68 Poa fish Poa mach Glassogobius giuris

17 Bitter gourd Karala pata* Momordica charantia 43 Fekong Fakong Alpinia nigra EGGS

18 na Sabarang* Ajuga macrosperma FRUITS 69 Chicken egg (farm) Murgir dim (f) Gallus bankiva murghi

19 Roselle Amila pata* Hibiscus sabdariffa 44 Mango ripe(deshi) Paka Am Mangifera indica 70 Chicken egg (deshi) Murgir dim (d) Gallus bankiva murghi

20 na Lalam pata* Premna obtusifolia 45 Black berry (deshi) Kalojam Syzygium cumini 71 Duck egg Hasher dim Anas platyrhyncha

21 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa 46 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus MEAT

22 na Ojan shak* Spilanthes calva 47 Lichi (deshi)) Lichu Lichi sinensis 72 Chiken (farm) Farm murgi Gallus bankiva murghi

23 na Ghanda batali* Paederia foetida 48 Banana (ripe) Paka kala Musa sapientum 73 Chiken (deshi) Desi murgi Gallus bankiva murghi

24 na Orai balai Premna esculenta 49 Water melon Tormuz Citrullus vulgaricus 74 Beef Garor mangsha Beef cattle

25 Purslane Bat slai* Portulaca oleracea 50 Papaya (ripe) Paka pepey Carica papaya 75 Pork* Shukor Pot bellied pig

*ethnic food ** raw

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Table 3.12: Exclusive ethnic food list

English name Local name Scientific name

Leafy vegetables

1 Bitter gourd leaves Karala pata Momordica charantia

2 na Sabarang Ajuga macrosperma

3 Roselle Amila pata Hibiscus sabdariffa

4 na Lalam pata Premna obtusifolia

5 Indian Ivy-rue Baruna Shak Xanthoxylum rhetsa

6 na Ojan shak Spilanthes calva

7 na Ghanda batali Paederia foetida

8 na Orai balai Premna esculenta

9 Purslane Bat slai Portulaca oleracea

10 Yellow saraca Maytraba Saraca thaipingensis

non-LEAFY VEGETABLES

11 Pea eggplant Mistti begun Solanum spinosa

12 Solanum Tak begun Solanum virginianum

13 na Sigon data Lasia spinosa

14 Yam Pan/jhum alu Dioscorea pentaphylla

15 na Banchalta Dillenia pentagyna

16 na Fakong Alpinianigra

Fruits

17 Monkey jack Deuwa Artocarpus lakoocha

18 Burmese grape Lotkon Pirardia sapida

19 Wild Melon Sindera Cumis melo

20 na Roshko Syzygium balsameum

21 Bead tree Kusumgulu Elaeocarpus angustifolius

Meat

22 Pork Shukor Pot bellied pig

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General key foods

Pat shak

Potato Black berry

Deuwa

non -Leafy vegetables

Kalmi shak

Bael Pui shak Lotokon

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Ethnic key food items

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3.2 Collection of food sample

Procedure for collection of food sample is important to get reliable representative

nutrient values. Care should be taken to avoid risk of inadvertent moisture loss and

deterioration of nutrients during transport from the collection point to the lab.

The food samples that were collected from distant points such as field samples and

ethnic foods were water sprayed to keep moisten, well packed in clean dark plastic poly

bags to prevent water loss and damage by light, and then transported to lab within

shortest time span. Some activities of ethnic food sampling are shown in following

photographs.

Ethnic food collection

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Ethnic food collection

Ethnic food collection

Ehtnic food collection

Ehtnic food sorting

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Team member with DAE personnel

Ethnic food display

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3.3 Nutrient Compositions of Key Foods

Food composition database needs to be comprehensive. Its aim is to include food items

that are most commonly consumed by mass population for maintenance of their health

and nutrition. It is expected to be the primary source of nutritive information for food

policy program planning, designing dietary guideline, therapeutic diet formulation,

nutrition and agriculture research and training. By prioritizing the food items, the foods

that provide important nutrients for human nutrition as well as foods of public health

significance, are to be selected for analysis of nutrient profile and the analysis of every

sample for its content of all the nutrients is not required (Haytowitz et al, 2000).

Analysis of a range of foods contributing nutrients to the diet of human nutrition and

health is important. It is not truly possible, primarily, because a very large number of

foods form the human diet. The volume of analytical works and resource implications

for this work further make it impossible. Therefore, the strategy of prioritizing in

selecting food items and nutrients to be analysed has to be addressed properly.

In the present study, by prioritizing the consumption frequency and nutrient contribution

to the diet, seventy five key foods were selected for analysis of nutrient profile, which

contribute to human nutrition and which is of public health significance. The nutrient

profile included proximate nutrients and nutrients of health significance such as vitamin

C, carotenoids, β-carotene, and minerals. The results of proximate nutrients and

micronutrients are presented in the tables 3.13 to 3.24.

Foods, being biological materials, have variations in composition; therefore a database

cannot accurately predict the composition of any single sample of a food. It is especially

true for labile nutrients such as vitamin C, folates and carotenoids. As a result, FCD

cannot be used as literatures for comparision with values obtained for the foods

elsewhere. Nutrient values from one country are to be compared with values obtained in

other countries by reference to the original literature. However, FCD can be used as

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reference when the nutrient values are known to be based on original analytical values

(Greenfield and Southgate, 2003a).

The present study has analysed 75 key foods for their nutrient profile comprising 23

components grouped as proximate nutrients, vitamins, minerals and antinutrient. The

values obtained were reviewed and compared with the values reported in different food

composition databases such as- Dhesio Kgadder Pustiman (Ahmed et al, 1977), HKI –

Food CompositionTable (FCT) (Darnton-Hill et al, 1988), Nutritive values of Indian

Foods (IFCT: Indian Food Composition Tables; Gopalan et al, 2004), Thai FCT

(Puwastien et al, 1999) and with the values reported in literatures. It is to be noted that

some of the nutrients which have been analysed and included in this database are

missing or do not have in the other FCTs.

3.3.1 Proximate nutrients

The principal proximate nutrients are protein, fat and carbohydrate. They are oxidized in

the body to give energy. In addition to providing energy, the primary function of protein

is to supply amino acids for building body proteins. Fats, besides being a concentrated

source of energy, provide essential fatty acids having vitamin like function in the body.

Water is an essential element, with which the proximate principles form the bulk of the

diet. Dietary fibers are indigestible complex molecules, contribute to the bulk and have

some important function in the digestive tract.

The values obtained for proximate nutrients were found to be very much consistent with

those reported in other FCTs. For example, the protein values obtained in the present

study for some key foods were almost similar to those reported in the IFCT, DKPM and

Thai FCT (table 3.19). It is also consistent with literature data (Alam and

Rahman:http://www.cepis.org.pe/bvsacd/arsenico/arsenic/zahangir.pdf). Such as protein

value was 6.96g/100g ep for BRRI-29 rice (table 3.13) which ranges 6.4-7.4g/100g ep in

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IFCT, DKPM, Thai FCT. Somewhat similar outcomes were also obtained for other

proximate values in most of the key foods analysed.

3.3.2 Water in key foods

Water is an essential constituent in food composition database because water content is

one of the most variable components, particularly in plant foods. This variability affects

the composition of the food as a whole. The water content estimated in the key food

items was shown to be very much matched with those reported in other FCTs. Such as

moisture value in BRRI-29 rice is 12.14g/100 ep (table 3.13) which is almost same as

reported in the IFCT (13.3g/100g ep) and Thai FCT (11.2g/100g ep). Moisture content

in the other tested food items also has the comparable results.

3.3.3 Dietary fibre

Dietary fiber is the indigestible portion of plant foods. It has a number of physiological

functions and benefits including reduced appetite, lower variance in blood sugar levels,

reduced risks of heart disease, metabolic syndromes, diabetes, colorectal cancer and

constipation (http://en.wikipedia.org/wiki/Dietary_fiber). It also facilitates and improves

absorption of minerals. Therefore, information on dietary fiber content in plant foods is

important. This study has estimated dietary fiber content in a number of key foods.

Most of the values were consistent with the reported data; such as dietary fiber contents

in Amaranth leaves, Spinach leaves, Coriander leaves, Mint leaves, Carrot and

Pumpkin (table 3.23) were almost equivalent to those reported in IFCT and literatures

(http://www.dietary-fiber.info/; Punna et al, 2004).

3.3.4 Antinutrient-Phytate content

Phytic acid is a common constituent of many plant foods. It is a phytonutrient and has

antioxidant effect. Phytic acid, by binding with minerals, inhibits their absorption and

consequently induces mineral deficiencies to people who consume diet containing high

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phytate. Phytic acid as antioxidant is effective in prevention of colon cancer

(http://en.wikipedia.org/wiki/Phytic_acid).

In the present work, phytic acid content has been estimated for 35 key food items. The

data were compared with IFCT and also with literature value. It was indicated that some

values were matched with either IFCT or literature value and some did not. Such as

phytic acid content in Maize and Lentil were estimated to be 959.85 and 516.12mg/100g

ep (table 3.24) respectively which are almost same as reported elsewhere (Hidvegi and

Lasztity, 2002; Dost and Tokul, 2006) for the same foods. Phytic acid level estimated

for vegetables was also somewhat within the range as reported by Udosen and

Ukpanah (1993). Potato contained approximately same amount of phytic acid (16.36 vs

14.00mg/100g ep) as reported in IFCT.

3.3.5 Vitamins and minerals in key foods

Vitamins assist the enzymes that release energy from carbohydrates, proteins and fats.

Minerals are used for shaping of body structure and skeleton. They enhance the

immune system, support normal growth and development, and help cells and organs to

their functions. Vitamins and minerals are widely available from the natural foods we

eat.

Vitamin content Vitamins analysed in the key food items included carotenoid, vitamin C and beta-

carotene. There was a fairly good variation in the values obtained in this study as

compared to the other FCTs, but some of the values were found almost consistent. For

examples- carotenoid values for Sabuj Kochu Sak, Corriendar leaves, Mula Sak and

Mango (ripe) obtained in this study were estimated 8.35, 6.83, 4.22 and 2.56mg/100g

ep (table 3.14, 3.17) against the values 10.278, 6.918, 5.295 and 2.743mg/100g ep

respectively in the IFCT. Similarly the vitamin C values for Sweet potato, Black berry

and Amla in the present study was found to be 23.92, 65.58 and 434.05mg/100g ep

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respectively (table 3.17) while the values for the same foods were 24.00, 60.00 and

600mg/100g ep respectively in the IFCT. There was a wide variation in beta-carotene

value as compared to other FCTs. However, a few food items tested was found to have

a value of little variation as compared to IFCT value, such as the beta–carotene content

in Sobuj kochu shak was 7146.59µg/100g ep (table 3.23) as against a value

5920µg/100g ed in the IFCT. ,

Mineral content

Minerals are indispensable for normal life processes. They are required for metabolic

activities which are critical for cell differentiation and replication. Minerals, particularly

the trace elements, are essential to form endogenous antioxidant enzymes that are

required for endogenous antioxidant activity and immune modulation (Percival, 1998;

Shankar and Prasad, 1998).

A total of nine minerals –copper, zinc, iron, manganese, calcium, magnesium, sodium,

potassium and phosphorous were estimated in this study. Attempt has been made to

compare the values of these minerals with the data reported in other FCTs and

literatures. Some values are found to be consistent and some are inconsistent. It is

noted that most of the mineral content in rice, maize and lentil were found almost

matched with the data of IFCT and to some extent with Thai values. Calcium,

magnesium and phosphorous; and iron and manganes values for rice obtained in this

study were 12.75, 42.72 and 125.96 mg/100g ep and 990 and 612.45 µg/100g ed (table

3.14, 3.15) while these values are 9.0, 61.00 and 143mg/100g ep; and 1000.00 and 660

µg/100g ep respectively for the same foods in the IFCT. Similarly, copper value for

maize; calcium, magnesium. phosphorous, iron and manganese values for lentil were

also nearly consistent with IFCT values. In case of vegetables, copper value for Dheros

and Mistikumra; zinc value for Mistikumra, Carrot and Bael; iron value for Palong sak,

Begun, Potato, Sweet potato were almost same as compared to the IFCT value for

these foods.

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Table 3.13: Proximate nutrient composition of cereals and leafy vegetables

*ethnic na: not available

Sl. No. English name Bengali/Local name Scientific name Moisture Protein Fat FA CF Ash CHO Energy

g/100g edible portion Kcal/100g CEREALS

1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 12.14±0.03 6.96±0.08 0.31±0.00 0.26 0.24±0.00 0.60±0.01 79.75 349.63 2 Rice sunned* Atap chal Oryza sativa 12.98±0.13 7.74±0.04 0.43±0.00 0.37 0.26±0.00 0.54±0.01 78.11 347.29 3 Maize Vutta Zea mays 11.18±0.02 10.99±0.11 2.89±0.13 1.94 2.53±0.03 1.38±0.03 71.98 357.90

PULSE 4 Lentil (deshi) Masur dal Lens culinaris 11.38±0.13 23.91±0.13 0.73±0.01 0.7 0.69±0.02 2.63±0.01 60.66 344.85

LEAFY VEGETABLES 5 Joseph’s Coat Lalshak Amaranthus gangeticus 90.75±0.11 2.39±0.58 0.19±0.02 0.15 0.9± 0.02 1.42± 0.1 4.35 28.67 6 Spleen Amaranth Data shak Amaranthus dubius 91.40±0.22 2.36±0.55 0.30±0.02 0.24 0.88±0.01 0.93±0.04 4.13 28.66 7 Bottle Gourd Lau shak Lagenaria siceraria 92.82±0.30 2.58±0.70 0.22±0.01 0.18 1.17±0.02 2.19±0.12 1.02 16.38 8 Radish Mula shak Raphanus sativus 95.33±0.86 1.82±0.23 0.25±.02 0.2 0.62±0.01 1.12±0.22 0.85 12.97 9 Coco-yam Sobuj kochu shak Colocasia esculenta 89.29±0.40 2.45±0.92 0.41±0.02 0.33 0.77±0.03 2.14±0.16 4.94 33.25 10 Jute Pat shak Corchorus capsularis 85.70±0.07 5.2±0.95 0.63±0.02 0.50 1.36±0.52 2.31±0.05 8.47 60.35 11 Indian spinach Poi shak Basella alba 93.84±0.02 1.5±0.65 0.22±0.01 0.18 0.54±0.03 0.99±0.04 2.91 19.62 12 Spinach Palong shag Spinacia oleracea 89.93±0.07 2.26±1.11 0.21±0.04 0.17 0.73±0.01 2.12±0.06 4.75 29.93 13 Swamp morning-glory Kalmi shak Ipomoea aquatica 92.32±0.12 1.99±0.80 0.32±0.04 0.26 0.95±0.01 0.63±0.10 3.79 26.00 14 Thankuni Thankuni Pata Centella asiatica 81.84±0.06 2.3±1.30 0.85±0.01 0.68 0.90±0.02 1.70±0.17 12.41 66.49 15 Corriander Dhane pata Coriandrum sativum 88.99±0.33 3.04±1.00 0.23±0.05 0.18 0.99±0.03 2.17±0.18 4.58 32.55 16 Spearmint Pudina pata Mentha viridis 87.16±0.49 3.07±1.02 0.42±0.03 0.34 1.36±0.02 1.23±0.11 6.76 43.10 17 Bitter gourd Karola pata* Momordica charantia 91.57±0.14 2.13±0.11 0.15±0.00 0.12 0.62±0.01 1.70±0.09 3.83 25.19 18 na Sabarang* Ajuga macrosperma 88.63±0.24 2.57±0.06 1.29±0.08 1.03 1.25±0.06 1.7±0.05 4.56 40.13 19 Roselle Amila pata* Hibiscus sabdariffa 90.56±0.21 2.86±0.02 1.53±0.10 1.22 1.20±0.03 0.75±0.04 3.10 37.61 20 na Lalam pata* Premna obtusifolia 86.91±0.08 3.38±0.08 1.30±0.06 1.04 1.79±0.06 2.18±0.06 5.44 42.98 21 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa 77.70±0.39 3.17±0.06 1.82±0.08 1.46 2.51±0.09 1.95±0.06 12.85 80.46 22 na Ojan shak* Spilanthes calva 89.03±0.08 3.10±0.03 1.08±0.11 0.86 1.31±0.05 1.92±0.06 3.56 36.36 23 na Ghanda batali* Paederia foetida 82.87±0.52 2.90±0.02 2.84±0.01 2.27 3.41±0.10 1.79±0.07 6.19 61.92 24 na Orai balai Premna esculenta 78.81±1.16 4.22±0.03 2.44±0.01 1.95 3.71±0.05 3.05±0.23 7.77 69.92 25 Purslane Bat slai* Portulaca oleracea 91.68±0.34 1.95 ± 0.03 0.66±0.02 0.53 0.89±0.04 2.12±0.06 2.70 24.54 26 Yellow saraca Maytraba Saraca thaipingensis 78.72±1.12 7.80 ± 0.12 2.79±0.08 2.23 2.70±0.06 2.46±0.11 5.53 78.43

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Table 3.14: Vitamin C, carotenoids and micromineral composit ion of cereals and leafy vegetables

nd: not done na: not available *ethnic

Sl.No.

English name Bengali/Local name Scientific name Vitamin C Carotenoids Copper Zinc Iron Manganese

µg/100g edible portion CEREALS

1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa nd nd 510.00±17.13 310.31±19.72 990±10 612.45±19.23 2 Rice sunned* Atap chal Oryza sativa nd nd 490.13±11.31 740.49±10.17 910±10 592.27±31.79 3 Maize Vutta Zea mays nd nd 430.21±13.99 400.90±27.90 1310±20 552.97±18.31

PULSE 4 Lentil (deshi) Masur dal Lens culinaris nd nd 1620.91±80.12 6040.71±99.8 6130.53±50.29 987.83±37.19

LEAFY VEGETABLES 5 Joseph’s Coat Lalshak Amaranthus gangeticus 22.55±0.35 4.31±0.03 444.03±11.01 1128.58±1.82 2368.18±2.02 4995.37±4.72 6 Spleen Amaranth Data shak Amaranthus dubius 26.32±5.68 4.45±0.78 87.55±1.13 977.31±2.99 2897.03±2.97 4205.93±10.04 7 Bottle Gourd Lau shak Lagenaria siceraria 22.2±1.78 3.05±0.02 157.71±2.92 659.5±0.93 2107. 53±2.02 243.73±4.12 8 Radish Mula shak Raphanus sativus 68.85±0.73 4.22±0.16 89.34±1.27 457.85±2.98 904.52±1.00 89.34±2.16 9 Coco-yam Sobuj kochu shak Colocasia esculenta 60.09±5.20 8.35±0.10 226.74±3.85 684.49±1.20 10 05.35±0.01 1155.08±4.00 10 Jute Pat shak Corchorus capsularis 54.43±1.27 9.14±0.14 20.99±1.00 1469.27±1.0 9715.14±2.00 1619.19±1.00 11 Indian spinach Poi shak Basella alba 55.59±3.95 8.17±0.06 49.29±1.91 431.33±1.18 985.83±2.00 739.37±0.82 12 Spinach Palong shag Spinacia oleracea 22.44±2.93 4.35±0.07 60.24±2.05 512.01±2.00 1566.26±3.86 1430.72±2.11 13 Swamp morning-glory Kalmi shak Ipomoea aquatica 41.83±4.90 5.66±0.10 2010.74±0.14 767.46±2.68 1089.79±0.96 414.43±1.99 14 Thankuni Thankuni Pata Centella asiatica 37.77±1.68 7.47±0.21 508.17±2.02 2431.94±1.97 3702.36±2.56 2250.45±1.40 15 Corriander Dhane pata Coriandrum sativum 76.56±4.47 6.83±0.03 1233.48±2.01 1585.9±5.01 4977.97±3.00 462.56±8.08 16 Spearmint Pudina pata Mentha viridis 57.03±5.60 7.61±0.02 183.97±2.00 1760.84±0.03 3968.46±2.02 289.09±2.00 17 Bitter gourd Karola pata* Momordica charantia 107.90±3.40 10.47±0.49 66.45±2.13 865.95±2.99 1348.88±2.01 99.92±5.97 18 na Sabarang* Ajuga macrosperma 12.92±0.03 5.97±0.15 1159.09±0.01 522.62±0.47 2818.18±0.00 1659.09±0.00 19 Roselle Amila pata* Hibiscus sabdariffa 16.08±0.37 4.41±0.08 1026.61±0.41 513.31±0.31 3954.37±0.00 2737.64±0.73 20 na Lalam pata* Premna obtusifolia 18.86±0.09 3.03±0.13 1396.57±0.50 1554.67±0.00 3847.17±0.88 4295.13±0.88 21 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa 38.04±1.81 6.11±0.27 312.53±0.52 312.45 0.05 4375.00±0.01 12946.43±0.02 22 na Ojan shak* Spilanthes calva 15.11±0.02 4.61±0.38 351.26±0.00 461.03±0.03 2634.47± 0.40 2678.38±0.06 23 na Ghanda batali* Paederia foetida 7.36±0.02 6.99±0.10 305.02±0.01 135.59±0.01 3423.73±1.00 4779.66±0.29 24 na Orai balai Premna esculenta 22.94±2.82 4.45±0.78 253.69±0.58 1818.19±0.09 3551.79±0.80 175.48±0.21 25 Purslane Bat slai* Portulaca oleracea 3.24±0.05 2.24±0.14 215.77±0.01 414.94±0.04 27 21.99±0.00 2356.86±0.09 26 Yellow saraca Maytraba Saraca thaipingensis 92.6±0.00 13.18±1.15 251.57±0.01 1006.29±0.21 1425.58±0.43 1299.79±0.11

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Table 3.15: Macromineral composition of cereals and leafy v egetables

Sl.No. English name Bengali/Local name Scientific name Calcium Magnesium Sodium Potassium Phosphorous mg/100gm edible portion

CEREALS 1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 12.75±0.61 42.72±0.81 10.97±0.01 109.89±0.06 125.96±0.4 2 Rice sunned* Atap chal Oryza sativa 11.67±0.38 43.29±1.38 5.43±0.01 108.62±0.17 140.67±6.69 3 Maize Vutta Zea mays 21.53±0.56 176.76±5.22 13.79±0.08 248.4±1.58 28 1.99±1.92

PULSE 4 Lentil (deshi) Masur dal Lens culinaris 66.12±2.47 104.16±1.82 33.15±0.05 561.18±0.08 313.26±3.44

LEAFY VEGETABLES 5 Joseph’s Coat Lalshak Amaranthus gangeticus 90.75±0.01 27.94±0.95 83.26±.01 277.52±1.01 41. 63±1.01 6 Spleen Amaranth Data shak Amaranthus dubius 104.89±0.01 25.74±1.87 78.53±0.94 261.78±0.93 34.9±0.91 7 Bottle Gourd Lau shak Lagenaria siceraria 85.66±1.96 18.49±1.20 35.84±1.16 222.22±1.01 26 .89±0.91 8 Radish Mula shak Raphanus sativus 83.92±1.93 14.13±1.00 83.75±1.04 223.34±0.85 22 .33±0.95 9 Coco-yam Sobuj kochu shak Colocasia esculenta 77.75±1.90 26.1±0.91 53.48±0.99 374.33±1.09 42. 78±0.81

10 Jute Pat shak Corchorus capsularis 132.98±1.99 41.83±0.85 59.97±0.99 224.89±0.99 59.97±0.97 11 Indian spinach Poi shak Basella alba 55.14±0 .92 19.1±1.01 104.74±1.03 110.91±1.07 18.48±0.91 12 Spinach Palong sag Spinacia oleracea 47.65±1.90 22.36±0.84 248.49±1.10 173.19±1.33 24.47±1.04 13 Swamp morning-glory Kalmi shak Ipomoea aquatica 34.08±1.99 16.42±0.81 107.44±1.06 207.21±0.77 36.45±1.10 14 Thankuni Thankuni Pata Centella asiatica 147.37±1.89 50.09±1.00 199.64±1.02 508.17±0.99 45.37±0.90 15 Corriander Dhane pata Coriandrum sativum 113.33±1.80 28.19±0.92 121.15±0.99 396.48±0.90 30.29±0.90 16 Spearmint Pudina pata Mentha viridis 110.12±0.98 33.25±0.86 78.84±1.00 354.8±0.90 36 .14±0.96 17 Bitter gourd Karala pata* Momordica charantia 170.94±1.99 22.9±0.99 66.61±0.86 258.12±0.98 22 .9±0.91 18 na Sabarang* Ajuga macrosperma 49.34±0.01 0.0±0.00 0.40±0.02 268.18±1.00 52.2 7±1.0 19 Roselle Amila pata* Hibiscus sabdariffa 30.57±0.50 0.49±0.00 0.31±0.02 144.50±0.50 38 .02±0.00 20 na Lalam pata* Premna obtusifolia 35.84±0.05 0.00±0.0 0.45±0.03 376.81±0.00 44.79±0.00 21 Indian Ivy-rue Baruna Shak* Xanthoxylum rhetsa 84.82±0.10 0.0±0.00 0.67±0.02 348.21±0.20 44.7 9±0.26 22 na Ojan shak* Spilanthes calva 26.23±0.19 0.00±0.00 0.46±0.02 338.08±0.01 50.5 8±0.44 23 na Ghanda batali* Paederia foetida 64.51±0.00 0.07±0.00 0.51±0.04 298.31±0.31 40.85±0.17 24 na Orai balai Premna esculenta 54.41±0.01 0.13±0.00 0.88±0.04 600.42±0.43 43.65±1.37 25 Purslane Bat slai* Portulaca oleracea 20.28±0.20 8.30±0.00 0.50±0.02 285.47±0.70 24.3 4±1.11 26 Yellow saraca Maytraba Saraca thaipingensis 39.83±0.19 2.64±0.01 0.71±0.04 469.60±0.59 109.94±0.93

na: not available *ethnic

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Table 3.16: Proximate composition of roots & tuber, non-leafy ve getables and fruits Sl.no English name Bengali/Local name Scientific name Moisture Protein Fat FA CF Ash CHO Energy

g/100g edible portion Kcal/100g ROOTS & TUBER

27 Potato Gol Alu Solanum tuberosum 79.65±0.15 2.07±0.03 0.62±0.01 0.5 0.36±0.01 0.76±0.01 16.54 80.02 28 Sweet potato (red) Misti alu Ipomoes batatas 65.05±0.07 1.17±0.03 0.29±0.01 0.23 0.78±0.01 1 .05±0.02 31.66 133.93 29 Carrot Gazor Daucus carota 89.67±0.40 0.81±0.03 1.00±0.03 0.8 0.57±0.02 0.92±0.09 10.33 41.32

NON-LEAFY VEGETABLES 0 Egg plant Begun Solanum melongena 93.42±0.05 1.21±0.81 0.05±0.00 0.04 0.74±0.01 1.14±0.09 3.44 19.05 31 Bitter Gourd Karola Momordica charantia 93.91±0.39 1.11±0.54 0.07±0.01 0.06 1.16±0.07 0 .87± 0.02 2.88 16.59 32 Sweet pumpkin Misti kumra Cucurbita maxima 93.33±0.04 0.59±0.03 0.08±0.00 0.06 0.23±0.06 0 .67±0.07 5.10 23.48 33 Kakrol Kakrol Momordica cochinchinensis 89.33±0.58 1.47±0.22 0.10±0.00 0.08 1.55±0.04 1 .25±0.02 6.30 31.98 34 Ladies finger Dherosh Abelmoschus esculentus 92.65±0.08 1.31±0.22 0.19±0.02 0.15 0.57±0.00 1.19±0.06 4.09 23.31 35 Green papaya Kacha papay Carica papaya 93.85±0.03 0.60±0.20 0.05±0.00 0.04 0.64±0.01 1 .32±0.02 3.54 17.01 36 Folwal Potol Trichosanthes dioica 92.89±0.14 1.31±0.02 0.07 0.00 0.06 1.44±0.02 0.58±0.02 4.29 23.03 37 Green chilli Kacha marich Capsicum frutescens 84.83±0.18 2.86±0.95 0.83±0.04 0.66 4.9±0.14 1.13±0.16 5.45 40.71 38 Pea eggplant Mistti begun* Solanum spinosa 84.43±0.40 2.45±0.03 2.13±0.21 1.70 4.21±0.07 1.12±0.04 5.66 51.61 39 Solanum Tak begun* Solanum virginianum 78.94±0.16 2.70±0.03 5.27±0.35 4.22 6.97±0.06 1. 57±0.03 4.55 76.43 40 Sigon data Sigon data* Lasia spinosa 96.09±0.25 0.66±0.02 0.32±0.01 0.26 0.59±0.05 0.83±0.02 1.51 11.56 41 Yam Pan/jhum alu* Dioscorea pentaphylla 66.05±0.59 2.69±0.04 1.17±0.13 0.14 1.72±0.05 1. 14±0.11 28.23 125.21 42 Banchalta Banchalta* Dillenia pentagyna 89.58±0.30 2.12±0.03 0.63±0.01 0.51 1.22±0.06 1.34±0.10 5.11 34.59 43 Fekong Fakong Alpinia nigra 97.00±0.11 0.44±0.02 0.27±0.01 0.22 0.89±0.04 0. 79±0.03 0.61 6.63

FRUITS 44 Mango ripe (deshi) Paka Am Mangifera indica 86.84±0.28 0.61±0.20 0.63±0.07 0.50 0.73±0.85 0 .35±0.04 10.84 51.47 45 Black berry (deshi) Kalojam Syzygium cumini 86.32±0.04 0.62±0.07 0.27±0.02 0.22 1.08±0.06 1.05±0.20 11.66 51.55 46 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 77.88±0.53 1.53±0.07 0.14±0.02 0.11 0.58±0.12 0 .79±0.03 19.08 83.70 47 Lichi (deshi)) Lichu Lichi sinensis 83.70±0.36 1.26±0.09 0.93±0.07 0.74 0.66±0.20 0.8±0.06 13.45 67.21 48 Banana (ripe) Paka kala Musa sapientum 74.56±0.38 1.31±0.07 0.36±0.05 0.29 0.26±0.02 0 .97±0.05 22.54 98.64 49 Water melon Tormuz Citrullus vulgaricus 92.97±0.47 0.73±0.09 0.20±0.00 0.16 0.09±0.03 0.36±0.01 5.65 27.32 50 Papaya (ripe) Paka papay Carica papaya 91.14±0.58 0.61±0.10 0.14±0.01 0.11 0.74±0.03 0 .53±0.04 6.84 31.06 51 Amla Amloki Emblica officinalis 82.52±0.12 0.60±0.19 0.12±0.01 0.10 0.85±0.00 1.18±0.10 14.73 62.40 52 Melon (mix) Bangi/futi Cucumus melo 95.02±0.33 0.19±0.06 0.21±0.00 0.17 0.17±0.45 0 .25±0.03 4.16 19.29 53 Wood apple Bael Aegle marmelos 61.86±0.55 3.55±0.06 2.56±0.07 2.05 1.33±0.01 0.22±0.05 30.48 159.16 54 Palm (ripe) Paka tal Borassus flabellifer 81.21±0.11 0.66±0.08 0.42±0.03 0.34 0.97±0.07 0 .92±0.03 30.48 159.16 55 Pineapple (jaldogi) Anarosh Ananas comosus 85.08±0.18 0.61±0.10 0.58±0.02 0.46 1.06±0.02 0.45±0.01 12.22 56.54 56 Monkey jack* Deuwa* Artocarpus lakoocha 60.74±0.75 1.97±0.01 8.73±0.16 6.70 3.63±0.06 0 .98±0.12 24.31 180.45 57 Burmese grape* Lotkon* Pirardia sapida 90.54± 0.49 1.61±0.20 2.49±0.10 1.99 4.2±0.18 0.52±0.06 0.64 31.41 58 Wild Melon Sindera* Cumis melo 95.88±0.11 0.36±0.03 0.52±0.05 0.42 0.79±0.03 0. 54±0.02 1.91 13.76 59 na Roshko* Syzygium balsameum 87.12±0.25 0.70±0.03 1.54±0.01 1.23 1.32±0.07 1. 33±0.08 7.99 48.62 60 Bead tree kusumgulu* Elaeocarpus angustifolius 92.51±0.08 0.95±0.03 0.94±0.13 0.75 0.88±0.09 0. 81±0.03 3.91 27.90

na: not available *ethnic

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Table 3.17: Vitamin C, carotenoids and micromineral composition of roots & tuber, non-leafy vegetables Sl.No. English name Bengali/Local name Scientific name Vitamin C Carotenoids Copper Zinc Iron Manganese

mg/100g edible µg/100g edible portion ROOTS & TUBER

27 Potato Gol Alu Solanum tuberosum 8.80±0.84 nd 290.00±10.00 790.00±10.00 400.00±0.00 10.43 ±1.05 28 Sweet potato (red) Misti alu Ipomoea batatas 23.92±3.09 0.25±0.02 100.00±20.00 170.00±30.00 250.00±0.00 12.31±2.51 29 Carrot Gazor Daucus carota 11.17±0.76 8.56±0.49 53.72±0.98 327.39±0.05 638.56±20.33 3.82 ±0.84

NON-LEAFY VEGETABLES 30 Egg plant Begun Solanum melongena 6.66±0.58 nd 184.09±1.01 197.24±3.00 289.28±0.90 65.75 ± 1.8 31 Bitter Gourd Karola Momordica charantia 136.39±10.46 1.79±0.03 182.04±1.99 388.35±0.15 400.49±5.00 254.85 ± 6.07 32 Sweet pumpkin Misti kumra Cucurbita maxima 12.12±0.41 3.81±0.13 40.03±1.00 306.87±3.97 400 .27±2.02 0.13 ± 0.01 33 Kakrol Kakrol Momordica cochinchinensis 119.06±7.01 0.27±0.06 2401.66±4.00 476.19±4.01 538.3±2.00 62.11± 2.08 34 Ladies finger Dherosh Abelmoschus esculentus 10.18±1.10 0.38±0.03 103.93±2.00 430.59±2.01 282.11±3.01 29.7 ± 2.04 35 Green papaya Kacha papay Carica papaya 13.74±0.42 nd 24.59±1.00 258.14±2.00 417.95±3.01 9.83 ± 1.86 36 Folwal Potol Trichosanthes dioica 44.18±1.81 nd 70.39±1.00 239.44±1.22 309.86±1.01 84.51±1.31 37 Green chilli Kacha marich Capsicum frutescens 101.0±12.22 1.01±0.06 1832.06±3.01 1190.84±0.09 4488.55±2.00 183.21± 2.04 38 Pea eggplant Mistti begun* Solanum spinosa 6.99±0.30 3.62±0.18 305.35±0.01 122.14±0.01 213.74±0.29 549.62 ± 0.02 39 Solanum Tak begun* Solanum virginianum 16.66±0.105 4.58±0.24 345.57±0.50 302.38±0.32 1857.45±0.00 734.34 ± 0.33 40 Sigon data Sigon data* Lasia spinosa 2.63±0.06 0.95±0.01 91.22±0.20 224.56±0.50 196 .49±0.00 1340.35 ± 0.35 41 Yam Pan/jhum alu* Dioscorea pentaphylla 19.25±0.24 0.48±0.01 1152.54±0.43 338.98±0.02 1084.75± 0.00 610.17 ± 0.17 42 Banchalta Banchalta* Dillenia pentagyna 31.16±1.11 15.17±0.04 447.28±0.01 575.08±0.89 660.28±0.28 1853.04 ± 0.00 43 Fekong Fakong Alpinia nigra 3.24±0.07 0.13±0.01 89.53±0.01 131.30±0.71 537.15±0.06 1020.60 ± 0.01

FRUITS 44 Mango ripe(deshi) Paka Am Mangifera indica 10.88±1.20 2.56±0.26 173.23±2.03 543.31±1.81 1312.34±2.61 577.43± 0.96 45 Black berry (deshi) Kalojam Syzygium cumini 65.58±7.04 0.39±0.11 116.42±2.00 1090.52±0.30 1758.14±1.96 147.3 ± 1.95 46 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 11.08±0.50 0.71±0.13 30.5±1.00 566.45±2.95 915.03±3.00 261.44 ± 4.21 47 Lichi (deshi)) Lichu Lichi sinensis 0.07±0.00 0.07±0.00 251.63±2.16 522.88±3.02 1013.07±2.00 88.35±3.16 48 Banana (ripe) Paka kala Musa sapientum 15.65±4.21 nd 45.92±2.00 714.29±1.19 1122.45±2.95 204.08 ± 0.99 49 Water melon Tormuz Citrullus vulgaricus 3.84±0.65 4.20±0.22 80.81±3.02 176.43±3.66 498. 32±1.82 78.11± 2.20 50 Papaya (ripe) Paka papay Carica papaya 7.48±2.65 2.33±0.27 1431.11±2.00 2933.33±2.00 145.78±1.00 186.67±3.98 51 Amla Amloki Emblica officinalis 434.05±27.31 nd 8146.85±0.95 734.27±1.15 1153.85±2.00 104.9 ± 2.00 52 Melon (mix) Bangi/futi Cucumus melo 3.65±1.33 0.80±0.13 61.87±3.00 62.71±3.94 245.76±0.10 177.97± 2.99 53 Wood apple Bael Aegle marmelos 15.67±2.12 0.15±0.01 2031.41±2.01 432.81±2.03 2233.86±2.00 202.44± 2.08 54 Palm (ripe) Paka tal Borassus flabellifer 35.13±0.21 3.57±0.09 4172.93±3.02 413.53±1.96 1240.6±10.00 150.37±4.23 55 Pineapple (jaldogi) Anarosh Ananas comosus 27.82±3.20 0.71±0.02 240±5.00 601.48±2.31 1600± 10.00 671.16±4.69 56 Monkey jack* Deuwa* Artocarpus lakoocha 11.68±1.65 4.13±0.46 796.18±1.01 3980.89±0.04 5254.78±1.00 549.36± 0.73 57 Burmese grape* Lotkon* Pirardia sapida 12.05 ± 1.60 0.12±0.01 248.14±1.98 903.23±2.00 1488.83±1.93 1091.81± 9.97 58 Wild Melon Sindera* Cumis melo 9.95±0.23 1.84±0.04 32.86±0.37 32.87±0.02 156. 12±0.00 49.30 ± 0.29 59 na Roshko* Syzygium balsameum 13.12±0.07 1.19±0.04 128.58±0.56 0.13±0.01 0.26±0.01 154.04 ± 0.00 60 Bead tree kusumgulu* Elaeocarpus angustifolius 6.08±0.23 0.26±0.01 344.05±0.01 403.89±0.01 1780.10±0.10 299.18 ± 0.09

nd: not done na: not available *ethnic

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Table 3.18: Macromineral composition of roots and tuber and non- leafy vegetables

Sl.No. English name Bengali/Local name Scientific name Calcium Magnesium Sodium Potassium Phosphorous mg/100gm edible portion

ROOTS & TUBER 27 Potato Gol Alu Solanum tuberosum 9.38±0.68 41.96±1.22 10.42±0.03 403.61±0.41 43. 3±0.14 28 Sweet potato (red) Misti alu Ipomoes batatas 47.09±3.44 33.59±2.03 10.85±0.03 304.0±0.82 38.2±0.14 29 Carrot Gazor Daucus carota 0.23±0.00 5.03±0.00 68.26±0.01 87.46±3.27 1.10±0.25

NON-LEAFY VEGETABLES 30 Egg plant Begun Solanum melongena 7.23± 2.07 11.51±0.95 32.87±1.10 157.79±1.16 19 .72± 0.74 31 Bitter Gourd Karola Momordica charantia 10.92±1.93 14.32±0.98 36.41±1.99 182.04±1.00 19.72±0.90 32 Sweet pumpkin Misti kumra Cucurbita maxima 13.74±2.92 3.54±0.86 26.68±0.95 120.08±0.94 13. 34±0.91 33 Kakrol Kakrol Momordica cochinchinensis 9.83±1.84 19.57±0.98 51.76±1.11 186.34±0.83 25.88±0.89 34 Ladies finger Dherosh Abelmoschus esculentus 45.95±1.96 19.67±0.79 37.12±1.00 178.17±1.00 27 .84±0.86 35 Green papaya Kacha papay Carica papaya 17.76±1.90 13.09±1.00 43.02±1.01 129.07±1.02 15.37±1.07 36 Folwal Potol Trichosanthes dioica 17.32±1.98 15±1.00 28.17±1.02 147.89±0.90 17.61±0.89 37 Green chilli Kacha marich Capsicum frutescens 12.21±2.09 27.94±0..95 76.34±1.00 274.81±1.06 38.17±1.86 38 Pea eggplant Mistti begun* Solanum spinosa 26.81±0.21 0.65±0.02 0.40 ± 0.03 277.86 ±0.10 63.05±1.98 39 Solanum Tak begun* Solanum virginianum 19.27±0.75 4.49±0.40 0.52 ± 0.04 336.96 ±0.00 69.83±0.71 40 Sigon data Sigon data* Lasia spinosa 1.64±0.29 0.49±0.00 0.20 ±0.03 147.32 ± 0.32 19.48±0.53 41 Yam Pan/jhum alu* Dioscorea pentaphylla 1.89±0.40 17.09± 0.01 0.78±0.04 352.54 ±0.0 34. 90±1.00 42 Banchalta Banchalta* Dillenia pentagyna 15.98±0.00 0.64±0.29 0.47±0.02 287.54 ±0.45 40.72±0.26 43 Fekong Fakong Alpinia nigra 1.48±0.08 033±0.00 0.21±0.02 134.89±0.00 19.22±1.32

FRUITS 44 Mango ripe (deshi) Paka Am Mangifera indica 16.08±1.99 6.69±1.01 2.79±0.52 98.48±1.00 7.74±0.82 45 Black berry (deshi) Kalojam Syzygium cumini 26.73±1.84 11.99±1.00 50.57±3.14 106.91±1.01 11. 64±0.86 46 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 12.64±1.84 26.8±0.89 87.15±1.05 305.01±1.00 10.89±0.90 47 Lichi (deshi)) Lichu Lichi sinensis 20.83±0.97 5.15±1.10 119.21±5.83 134.8±1.00 15.77±0.89 48 Banana (ripe) Paka kala Musa sapientum 6.38±2.69 26.28±0.90 102.04±1.01 255.1±1.00 19.13±1.00 49 Water melon Tormuz Citrullus vulgaricus 13.47±0.97 4.01±1.73 31.94±3.68 58.92±0.94 6.36± 1.02 50 Papaya (ripe) Paka papay Carica papaya 15.11±1.93 6.62±1.02 11.85±0.93 133.33±1.00 11.02±0.98 51 Amla Amloki Emblica officinalis 13.81±1.85 8.08±0.99 69.93±5.95 174.83±1.05 13. 11±0.99 52 Melon (mix) Bangi/futi Cucumus melo 6.46±1.00 1.02±0.02 2.86±0.67 27.54±1.11 1.46±0.60 53 Wood apple Bael Aegle marmelos 70.68±0.98 16.58±1.27 6.92±0.53 427.57±1.19 23.04±1.03 54 Palm (ripe) Paka tal Borassus flabellifer 7.89±0.01 13.91±1.02 93.98±1.01 375.94±1.05 14.1±0.99 55 Pineapple (jaldogi) Anarosh Ananas comosus 24.82±0.94 12±2.00 41.81±2.97 122.22±1.00 6.82±0 .88 56 Monkey jack* Deuwa* Artocarpus lakoocha 66.68±2.01 23.69±0.90 79.17±6.11 348.33±1.01 22.69±0.94 57 Burmese grape* Lotkon* Pirardia sapida 52.11±2.07 11.29±0.95 7.21±1.07 198.51±1.00 17.12±0.99 58 Wild Melon Sindera* Cumis melo 4.27±0.25 1.13±0.09 0.18±0.01 66.74 ±0.00 28.16±1.86 59 na Roshko* Syzygium balsameum 8.19±0.01 5.96±0.09 0.39±0.03 256.74 ± 0.22 39. 53±1.01 60 Bead tree kusumgulu* Elaeocarpus angustifolius 0.17±0.01 2.69±0.00 0.31±0.03 109.20 ±0.00 14. 66±1.19

nd: not done na: not available *ethnic

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Table 3.19: Proximate composition of fish, egg and meat

Sl.No. English name Bengali/Local name Scientific name Moisture Protein Fat FA Ash CHO Energy g/100g edible portion Kcal/100g

FISHES 61 Carp Ruhi Labeo rohita 75.63±0.67 15.60±0.38 5.07±0.10 3.55 0.57±0.06 3.13 120.55 62 Tilapia Tilapia mach Anabus testudineus 73.92±0.48 16.87±0.30 5.11±0.08 3.58 0.59±0.01 3.51 127.51 63 Dragon Fish Pangash Pangasius pangasius 71.91±1.64 13.71±0.10 11.95±0.21 8.37 0.47±0.01 1.96 170.23 64 Sunfish Mola mach Mola mola 76.29±0.49 12.96±0.13 6.39±0.04 4.47 1.61±0.06 2.75 120.35 65 Arguskala Kachki mach Scatophagus argus argus 80.73±0.10 12.99±0.07 2.13±0.06 1.49 1.09±0.08 3.06 83.37 66 Taki fish Taki mach Channa puncpatus 79.71±0.05 17.18±0.93 1.47±0.02 1.03 0.60±0.02 1.04 86.11 67 Silver Carp Silver Carp Hypophthalmichthys nobilis 75.45±0.20 14.59±0.17 6.10±0.12 4.27 0.52±0.03 3.34 126.62 68 Poa fish Poa mach Glassogobius giuris 77.69±0.43 15.52±0.26 3.46±0.08 2.42 0.51±0.02 2.82 104.50

EGGS 69 Chicken egg (farm) Murgir dim (farm) Gallus bankiva murghi 75.78±0.50 12.07±0.19 11.37±0.10 9.44 0.77±0.03 0.78 153.73 70 Chicken egg (deshi) Murgir dim (deshi) Gallus bankiva murghi 76.12±1.92 11.33±0.15 11.60±0.07 9.63 0.89±0.03 0.95 153.52 71 Duck egg Hasher dim Anas platyrhyncha 68.39±0.19 15.47±0.37 15.87±0.29 13.17 0.95±0.06 0.27 205.79

MEATS 72 Chiken (farm) Farm murgi Gallus bankiva murghi 74.61±1.88 16.29±0.34 5.65±0.36 5.34 1.13± 0.03 2.32 125.29 73 Chiken (deshi) Desi murgi Gallus bankiva murghi 74.92±0.62 15.61±0.43 3.05±0.51 2.88 0.72± 0.01 5.70 112.69 74 Beef Garor mangsha Beef cattle 75.67±0.53 12.49±0.25 8.64±0.12 8.16 1.03± 0.05 2.17 136.40 75 Pork* Shukor Pot bellied pig 47.96±0.73 11.49±0.30 38.72±0.80 36.05 1.59±0.09 1.83 401.76

*ethnic

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Table 3.20: Micromineral composition of fish, egg and meat

Sl no. English name Bengali/Local name Scientific name Copper Zinc Iron Manganese

µg/100g edible portion FISHES

61 Carp Ruhi Labeo rohita 1853.66±0.35 1110.73±67.62 1376.15±75.47 30.0 ±1. 99 62 Tilapia Tilapia mach Anabus testudineus 1566 .58±0.63 1403.19±144.17 1311.58±77.01 44.39±0.90 63 Dragon Fish Pangash Pangasius pangasius 1517.86±0.92 646.23±183.67 1277.92±107.33 38.62±0 .94 64 Sunfish Mola mach Mola mola 2508.31±0.31 3431.63±68.42 1338.57±12.05 60.57±1.07 65 Arguskala Kachki mach Scatophagus argus argus 1838.38±2.56 3108.31±49.86 1064.45±66.34 82.05±1.04 66 Taki fish Taki mach Channa puncpatus 1804.88±0.12 757.16±112.10 1173.21±8.18 33.94±1.0 5 67 Silver Carp Silver Carp Hypophthalmichthys nobilis 1679.46±0.50 903.66±186.90 1163.18±175.76 28.89±1 .40 68 Poa fish Poa mach Glassogobius giuris 2584.27±0.23 1188.31±12.66 1576.92±76.80 72.19±0.79

EGGS 69 Chicken (farm) Murgir dim (farm) Gallus bankiva murghi 1980.58±0.99 1171.45±0.35 1539.17±8.09 59.71±1.7 70 Chicken (deshi) Murgir dim (deshi) Gallus bankiva murghi 2383.42±1.02 2034.18±358.18 1653.98±46.18 56.22±1.0 71 Duck egg Hasher dim Anas platyrhyncha 3411.18 ±1.09 1405.57±1.55 2159.02±4.76 87.17±1.0 2

MEAT 72 Chiken (farm) Farm murgi Gallus bankiva murghi 2126.58±0.61 1292.25±69.77 1583.77±109.77 53.92±1 .54 73 Chiken (deshi) Desi murgi Gallus bankiva murghi 2436.55±3.45 1572.20±69.96 1467.14±0.00 62.18±0.9 9 74 Beef Garor mangsha Beef cattle 2776.70±1.13 1839.81±267.22 1385.72±34.87 143.93±0.98 75 Pork* Shukor Pot bellied pig 5738.22±0.07 2380.67±144.21 3412.80±324.96 156.02±1.02

*ethnic

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Table 3.21: Macromineral composition of fish, egg and meat

Sl.No

. English name Bengali/Local name Scientific name Calcium Magnesium Sodium Potassium Phosphorous

mg/100gm edible portion

FISHES

61 Carp Ruhi Labeo rohita 0.34±0.04 11.82±0.16 133.94±27.63 238.49±28.13 5.81±0.55

62 Tilapia Tilapia mach Anabus testudineus 0.56±0.06 12.85±0.05 128.45±0.04 245.97±11.92 7 .16±0.13

63 Dragon Fish Pangash Pangasius pangasius 0.42±0.02 6.05±4.19 103.47±18.80 169.21±30.58 4 .99±1.04

64 Sunfish Mola mach Mola mola 0.60±0.00 11.95±0.01 110.07±6.80 139.79±3.53 13.17±0.21

65 Arguskala Kachki mach Scatophagus argus argus 0.48±0.00 9.71±0.01 67.69±5.52 92.60±9.71 10.54±0.02

66 Taki fish Taki mach Channa puncpatus 0.49±0.00 10.01±0.04 88.58±0.01 165.93±9.37 5.48±0.26

67 Silver Carp Silver Carp Hypophthalmichthys nobilis 0.49±0.00 11.05±0.04 104.82±6.41 186.35±7.19 6.03±0.03

68 Poa fish Poa mach Glassogobius giuris 0.57±0.10 12.42±1.37 139.89±14.23 270.81±10.87 7.49±1.24

EGGS

69 Chicken (farm) Murgir dim (farm) Gallus bankiva murghi 0.43±0.005 10.07±0.12 126.33±6.82 90.06±4.86 5.25±0.15

70 Chicken (deshi) Murgir dim (deshi) Gallus bankiva murghi 0.48±0.01 10.57±0.12 134.75±7.21 96.80±4.39 6.41±0.44

71 Duck egg Hasher dim Anas platyrhyncha 0.53±0.005 10.66±0.07 133.96±9.14 85.14±5.57 6.00±0.65

MEAT

72 Chiken (farm) Farm murgi Gallus bankiva murghi 0.24±0.03 12.02±0.33 117.53±7.16 200.93±7.28 6.55±0.38

73 Chiken (deshi) Desi murgi Gallus bankiva murghi 0.25±0.01 12.27±0.00 131.90±7.18 234.50±2.94 7. 20±0.17

74 Beef Garor mangsha Beef cattle 0.16±0.02 10.69±0.43 92.04±13.70 145.12±23.69 3 .53±0.50

75 Pork* Shukor Pot bellied pig 0.69±.0.15 17.37±5.42 168.85±88.67 179.07±85.66 6.16±3.09 *ethnic

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Table 3.22: β-carotene content in general and ethnic foods

English name Local/Bengali/Ethnic Name

Botanical/Scientific name β-carotene µg%

edible portion LEAFY VEGETABLE S

1 Joseph’s Coat Lal shak Amaranthus gangeticus 1256.53

2 Spleen Amaranth Data shak Amaranthus dubius 4904.33

3 Coco-yam Sobuj kochu shak Colocasia esculenta 7146.59

4 Bottle Gourd Lau shak Lagenaria siceraria 2370.64

5 Indian spinach Pui shak Basella alba 1775.10

6 Swamp Morning-glory Kalmi shak Ipomoea aquatica 2383.68 14 Corriander leaves Dhane pata Coriandrum sativum 1470.54

7 na Sabarang* Ajuga Macrosperma 467.28

8 Roselle Amila pata* Hibiscus sabdariffa 1606.83

9 na Baruna Shak* Xanthoxylum rhetsa 1465.49

10 na Ojan shak/Surja kannya* Spilantses calva 1102.88

11 na Orai balai* Premna esculenta 1110.74

12 Yellow saraca Maytraba* Saraca thaipingensis 1486.42

13 na Ghanda batali* Paederia foetida 1708.97

ROOT & TUBERS AND non -LEAFY VEGETABLE S

15 Carrot Gazor Daucus carota 1689.43

16 Sweet pumpkin Misti kumra Cucurbita maxima 51.41

17 Kakrol Kakrol Momordica cochinchinensis 163.00

18 Banchalta* Banchalta* Dillenia pentagyna 55.47

FRUITS

19 Mango ripe(deshi) Paka Am Mangifera indica 356.28

20 Jack frujt (ripe) Paka Kathal Artocarpus heterophyllus 28.86

21 Papaya (ripe) Paka papay Carica papaya 425.77

22 Melon (mix) Bangi/futi Cucumus melo 663.68

23 Water melon Tormuz Citrullus vulgaricus 299.73

24 na Rashko* Syzygium balsameum 8.90

25 Bead tree kusumgulu* Elaeocarpus angustifolius 388.43

*ethnic food na: not available

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Table 3.23: Dietary fiber in key food items

Sl.No. English name Bengali/Ethnic

name Scientific name g/100g edible LEAFY VEGETABLES

1 Joseph’s Coat Lalshak Amaranthus gangeticus 4.23±0.53

2 Spleen Amaranth Data shak Amaranthus dubius 4.35±0.48

3 Bottle Gourd Lau shak Lagenaria siceraria 4.38±0.61

4 Radish Mula shak Raphanus sativus 2.58±0.18

5 Coco-yam Sobuj kochu shak Colocasia esculenta 2.90±0.75

6 Jute Pat shak Corchorus capsularis 5.75±0.03

7 Indian spinach Poi shak Basella alba 2.18±0.17

8 Spinach Palong shak Spinacia oleracea 2.92±0.21

9 Swamp morning-glory Kalmi shak Ipomoea aquatica 3.71±0.09

10 Thankuni Thankuni Pata Centella asiatica 8.66±1.07 11 Corriander Dhane pata Coriandrum sativum 5.92±0.15

12 Spearmint Pudina pata Mentha viridis 6.91±0.31

13 Bitter gourd Karola pata* Momordica charantia 2.25±0.59

14 Carrot Gazor Daucus carota 3.68±0.57

NON-LEAFY VEGETABLES

15 Egg plant Begun Solanum melongena 2.28±0.34

16 Bitter Gourd Karola Momordica charantia 0.41±0.02

17 Sweet pumpkin Misti kumra Cucurbita maxima 1.14±0.88 18 Kakrol Kakrol Momordica cochinchinensis 0.44±0.03

19 Ladies finger Dherosh Abelmoschus esculentus 3.10±0.41

20 Green papaya Kacha papay Carica papaya 2.71±0.28

21 Green chilli Kacha marich Capsicum frutescens 4.91±0.86

FRUITS

22 Mango ripe(deshi) Paka Am Mangifera indica 3.65±0.30

23 Black berry (deshi) Kalojam Syzygium cumini 7.25±0.82

24 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 5.14±1.06

25 Banana (ripe) Paka kala Musa sapientum 1.90±0.16

26 Water melon Tormuz Citrullus vulgaricus 1.61±0.80

27 Papaya (ripe) Paka papay Carica papaya 0.59±0.04

28 Amla Amloki Emblica officinalis

29 Melon (mix) Bangi/futi Cucumus melo 2.15±0.49

30 Wood apple Bael Aegle marmelos 6.96±2.33

31 Monkey jack Deuwa Artocarpus lakoocha 2.11±0.11

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Table 3.24: Phytic acid content in key food items

English name Bengali/Local name Scientific name Phytic acid mg%

edible portion CEREALS AND LENTIL 1 Rice parboiled (Brri-29) Sidhoy chal Oryza sativa 116.86 ± 5.43 2 Rice sunned** Atap chal Oryza sativa 147.97± 4.44 3 Maize Vutta Zea mays 959.85 ± 2.86 4 Lentil (deshi) Masur dal Lens culinaris 516.12 ± 9.1 LEAFY VEGETABLES 5 Joseph’s Coat Lalshak Amaranthus gangeticus 10.37±0.62 6 Spleen Amaranth Data shak Amaranthus dubius 16.4±1.40 7 Bottle Gourd Lau shak Lagenaria siceraria 3.31±0.04 8 Radish Mula shak Raphanus sativus 1.88±0.16 9 Coco-yam Sobuj kochu shak Colocasia esculenta 11.46±0.15 10 Jute Pat shak Corchorus capsularis 16.4±0.16 11 Swamp Morning-glory Kalmi shak Ipomoea aquatica 2.43±0.09 12 Thankuni Thankuni Pata Centella asiatica 3.41±0.12 13 Bitter gourd Karala pata* Momordica charantia 3.74±0.12 14 Roselle Amila pata* Hibiscus sabdariffa 15.90±0.07 non-LEAFY VEGETABLES 15 Egg plant Begun Solanum melongena 10.88±0.07 16 Bitter Gourd Karola Momordica charantia 8.27±0.29 17 Sweet pumpkin Misti kumra Cucurbita maxima 15.85±0.30

18 Kakrol Kakrol Momordica cochinchinensis

5.25±0.02

19 Ladies finger Dherosh Abelmoschus esculentus 5.98±0.02 20 Green papaya Kacha papay Carica papaya 7.72±0.07 21 Green chilli Kacha marich Capsicum frutescens 13.72±0.85 ROOTS & TUBERS 22 Potato Gol Alu Solanum tuberosum 16.36±0.04 23 Sweet potato (red) Misti alu Ipomoea batatas 20.25±0.15 24 Carrot Gazor Daucus carota 9.28±1.14 FRUITS 25 Mango ripe(deshi) Paka Am Mangifera indica 9.28±1.14 26 Black berry (deshi) Kalojam Syzygium cumini 10.05±0.06 27 Jackfruit (ripe) Paka Kathal Artocarpus heterophyllus 26.42±0.16 28 Banana (ripe) Paka kala Musa sapientum 18.34±2.74 29 Water melon Tormuz Citrullus vulgaricus 9.48±0.06 30 Papaya (ripe) Paka papay Carica papaya 26.83±0.06 31 Amla Amloki Emblica officinalis 8.20±0.49 32 Melon (mix) Bangi/futi Cucumus melo 19.82±0.10 33 Wood apple Bael Aegle marmelos 120.95±2.02 34 Monkey jack* Deuwa* Artocarpus lakoocha 30.9±0.43 35 Burmese grape* Lotkon* Pirardia sapida 13.57±1.27

*ethnic ** raw

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Table 3.25: Comparision of protein value in the present FCD with I FCT, DKPM, Thai FCT

pFCD: present Food Composition Database IFCT: Indian Food Composition Table DKPM: Deshio Khadder Pustiman Thai_FCT: Thai Food Composition Table

Sl.

no

Food items pFCD IFCT DKPM T-FCT Sl.

no

Food items cFCD IFCT DKPM T-FCT

Cereals Roots & Tubers

1 Rice parboiled 6.96 6.4 6.4 7.4 25 Potato 2.07 1.6 1.6 2.5

2 Rice (Atap) 7.74 na 6.8 na 26 Sweet Potato 1.17 1.2 1.2 0.9

3 Maize 10.99 11.1 11.1 na 27 Carrot 0.81 0.9 1.2 1.6

Pulses Fruits

4 Lentil (Deshi) 23.91 25.1 25.1 na 28 Mango (Ripe) 0.61 0.6 1.0 0.6

Leafy Vegetables 29 Jack fruit 1.53 1.9 1.8 1.7

5 Bottle Gourd 2.58 2.3 2.3 4.5 30 Papaya (Ripe) 0.62 0.6 1.0 0.5

6 Coco-yam 2.45 3.9 3.9 na 31 Black berry 0.61 na 1.9 na

7 Joseph’s Coat 2.39 2.8 3.3 na 32 Pine apple 0.61 0.4 0.9 0.4

8 Swamp Morning-glory 1.99 2.9 1.8 na 33 Banana (Ripe) 1.31 1.2 0.7 1.3

9 Indian spinach 1.5 na 2.2 na 34 Wood Apple 3.55 7.1 2.6 na

10 Jute leaves 5.2 na 2.6 na 35 Amla 0.6 na 0.9 na

11 Corandar leaves 3.04 3.3 3.3 2.3 36 Lichi 1.26 1.1 1.1 1.0

12 Mint leaves 3.07 4.8 2.9 na 37 Melon 0.19 0.3 0.3 na

13 Thankuni leaves 2.3 na 2.6 na 38 Water melon 0.73 0.2 0.2 0.6

14 Spleen Amaranth 2.36 na 1.8 na 39 Palm (Ripe) 0.66 0.7 0.7 0.5

15 Radish Shak 1.82 3.8 1.7 2.2 40 Eggs

16 Spinach 2.26 2 3.3 2.1 41 Chicken egg 12.07 13.3 13.3 12.8

17 Non Leafy Vegetables 42 Duck egg 13.5 13.5 15.47 12.1

18 Green chilli 2.86 na 1.6 1.4 Meat

19 Egg plant 1.21 1.4 1.8 na 43 Beef 12.49 22.6 22.6 na

20 Green papay 0.6 0.7 0.9 0.6 44 Chicken 16.29 26.6 25.9 17.3

21 Kakrol 1.47 na 2.1 na 45 Pork 11.49 18.7* 18.7 20.9

22 Ladies finger 1.31 1.9 1.8 na

23 Folwal 1.31 na 2.4 na

24 Sweet pumpkin 0.59 1.4 1.4 1.4

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Key Findings

Aim of this study was to prepare “A Food Composition Database for Bangladesh with

Special Reference to Selected Ethnic Foods”. In order to have this attempt done, the

study was designed to –identify key food items and analyses the key food for their

nutrient profile aiming at preparation of food composition database.

The key foods were identified through comprehensive food consumption survey (CFCS)

and focus group discussion (FGD). CFCS was conducted amongst 1210 general

households and 805 ethnic tribal households, and FGD was performed among Marma,

Chakma, Tanchanga and Tripura ethnic communities. Through CFCS and FGD 138

food items were identified, from which, 75 key food items were listed for analysis for

their nutrient profile. This key list comprised 53 food items consumed by both the

general and ethnic people; and 22 food items consumed by only the ethnic tribes.

The nutrient profile analysed for the 75 key foods comprised- proximate nutrients,

energy content; phytic acid , vitamin C, carotenoids, beta-carotene and minerals.

Comprehensive food consumption survey (CFCS)

• reveals food consumption pattern of general and ethnic population

• ethnic people consume almost all of the wild foods

• ethnic people consume most of the native general foods

• general people usually do not intake ethnic foods.

Key Foods

• A total of 138 food items comprising general and ethnic foods were identified.

• foods consumed by ≥ 5% households as well as nutrient dense foods of public

health significance were selected to make a list of 75 key foods for analysis of

their nutrient profile.

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Nutrient profile analysed

The selected seventy-five key foods were analysed for- proximate nutrients:

moisture, protein, total fat, fatty acid, carbohydrate, crude fiber, dietary fiber, ash;

energy content ; antinutrient: phytic acid; vitamins: vitamin C, carotenoids, beta-

carotene; and minerals: copper, zinc, iron, manganese, calcium, magnesium,

sodium, potassium and phosphorous.

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Policy Implications

Bangladesh has made major strides to meet the food needs of its increasing population

through boosting agricultural production. Agriculture produces above 90% of its food

need including cereals and vegetables, and to some extent fruits. It has been blessed

with high yielding varieties (HYV) of rice, plenty of vegetables and seasonal fruits, and

biologically rich open water fisheries. While cereal production is sufficient in Bangladesh

and certain vegetables and fruits are being exported.

The national food intake pattern in Bangladesh documents that people consume high

amount of cereal based diet and lesser amounts of micronutrient rich vegetable and

fruits. This results in an imbalanced diet habit. The changes in food chain with the

emergence of HYV newer foods as well as change in soil composition (due to

environmental changes, increased fertilizers use and crop intensity) have resulted in

possible changes in the nutrient composition of the foods being grown. Thus, the food

chain of the country has changed during the last decades. In addition, introduction of

western foods in Bangladesh markets has also changed the food habits. All these facts

call for a fresh analysis of the most frequently consumed foods.

Bangladesh does not have its own food composition database. This project is the start

point for development of a food composition database for Bangladesh.

This part food composition database (FCD) will provide the basis for planning food,

nutrition and health related policy tools. It will be the primary source of food composition

information for food and agriculture policy program planning. It will help in designing

• balanced diets

• food based dietary guidelines

• therapeutic diets

• health, nutrition and agriculture research

• nutrition education and training

• food security, safety, and regulations.

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A food composition database preferably includes all nutrients that are important in

human nutrition. It should consider the basic need for nutrient information, nutrient

contribution to public health nutrition, public health problems, nutrient of public health

significance, and importance of food trade need. In preparation of food composition

database, the improvement of analytical facilities should also be addressed.

It is noted that most of the databases have between 10 and 25 food groups comprising

hundreds to thousands of food items. The present database includes nutrient

composition of 75 food items incorporating 9 food groups. However, it is reference point

for developing and updating the food composition database.

Since Bangladesh is at the advent of preparation of its own food composition database,

consistent financial support should be ensured to have a national food composition

database with at least 500 food items consumed by mass population including ethnic

people.

Funding should also be provided to support lab upgradation for the analysis of

micronutrients of public health significance.

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Policy Recommendations

1. Updating FCT for Bangladesh

There is lack of up-to-date knowledge on food composition tables (FCT) for Bangladesh.

Given the major changes that have occurred in the complexity of the food chain as also

in the environment, soil composition, cropping patterns and cropping intensity, there is

need to update knowledge on the nutrient composition of most of the new high yielding

varieties of rice, wheat, maize, potatoes, fruits, vegetables, fish and livestock that have

become part of the nation’s production and consumption systems. There is need for

updating and constructing a revised FCT for use as tools in determining standard dietary

intake for different population groups including ethnic groups. The results of this

research can be a pilot contribution which needs to be built on for further work on FCT.

2. Constructing FCT of ethnic foods

Preparation of food composition tables for Bangladesh require research to ascertain the

extent to which the nutrient content of the new varieties of foods including ethnic and

traditional consumed by the tribal population contributes to the diets in Bangladesh. In

particular, the nutrient composition of the indigenous foods grown and consumed in the

Chittagong Hill Tracts (CHT) and other tribal areas is not known. The FCT for

Bangladesh needs to include the nutrient composition of ethnic foods and new FCT will

therefore need to be constructed.

3. Preparation of food based dietary guidelines (FBDGs)

To prepare dietary guidelines and determine standard dietary intake, the true nutrient

content of all foods consumed by the overall population needs to be known especially

ethnic groups. Knowing the profile of ethnic foods being produced and consumed is

critical in food and agriculture planning and in developing dietary guidelines. FCT on

ethnic foods can help inform agriculture, food and health policy on enhancing the supply

and demand for ethnic food sources which can serve as a valuable source of both

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macro and micronutrients. FBDGs for Bangladesh need to be elaborated and

implemented through a shared consensus and wider dissemination through efforts of

relevant stakeholders.

4. Strengthening collaboration for harmonization

Studies on the analyses of Bangladeshi foods have been carried out in the Institute of

Nutrition and Food Science (INFS), Institute of Food Science and Technology (IFST),

Institute of Public Health and Nutrition (IPHN), Bangladesh Agriculture University (BAU),

ICDDR,B and other research organizations. Collaboration among these institutions and

relevant GoB institutions such as BARC, BARI, BRRI and DAE should be strengthened.

There is an urgent need to consolidate and compile the FCT for Bangladesh through a

harmonization of the food composition analyses carried out over the years in these

institutions.

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Future Research

This study identifies a large number of food items that are consumed by mass

population including ethnics, but it has analysed only 75 key foods. The nutrient profile

of these 75 foods is not representative food items for a national food composition

database. Therefore, this attempt should be continued to prepare a national database

of at least 500 food items with a comprehensive nutrient profile.

Conclusion

The present report is a part of a wider food composition database. It provids newer

nutrient data of selected key foods. This database is expected to be the primary source

of nutrient values for food and agriculture policy planning, preparing dietary guidelines,

therapeutic diet formulation and research on nutrition, health and agriculture. It will be

useful to the policymakers and professionals who are working towards improving

nutrition and public health in Bangladesh.

This database will motivate future attempts for the analysis of nutrient profile of mass

peoples’ foods to develop a national food composition database.

A separate tabulation of nutrient data of the selected foods is also provided as a

reference output along with this report.

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Acknowledgements

This study was a collaborative research between the Institute of Nutrition and Food

Science, University of Dhaka and the Department of Pharmaceutical Chemistry, Faculty

of Pharmacy, University of Dhaka; Department of Agricultural Extension, Ministry of

Agriculture; Grain Quality and Nutrition Division, Bangladesh Rice Research Institute

(BRRI), Gazipur; Department of Biochemistry, Sher-e-Bangla Agriculture University; to

some extent- Nutrition Biochemistry Laboratory, ICDDR,B with the participation of fifteen

scientists including a number of post-graduates, M.Phil and PhD students who were

involved in designing, planning and carrying out this work. Thanks are due to the

Laboratory team who were involved in the analysis of nutrient profile of the key foods

and CFCS team who carried out the comprehensive consumption survey.

We are thankful to Professor Dr. Sagarmay Barua, Director, Institute of Nutrition and

Food Science, University of Dhaka for his whole hearted constant appreciation and

cooperation in carrying out and completetion of this work. Thanks are due to the

Director, Center for Excellence, University of Dhaka for providing some lab facilities, and

for allowing us to use the conference room. We are also indebted to Professor Dr. Md.

Aminul Haque Bhuyan of the Institute of Nutrition and Food Science, University of

Dhaka for his untiring suggestions and encouragement in carrying out this work.

We are obliged to the authorities of Dhaka University for enabling a silky-smooth end to

this work.

Special thanks are due to Mr. Paban Kumar Chakma, Agriculture Officer- Rangamati,

CHT and Mr. Gugal Chandra De, Agriculture Officer- Khagrachari, CHT as well as to the

other DAE staff for their sincere assistance in conducting the CFCS and the Focus

Group Discussions among the ethnic communities.

We express our gratefulness to Late Professor Dr. HKM Yusuf, Nutritionist, NFPCSP -

FAO; Dr. Lalita Bhattacharjee, Nutritionist, NFPCSP - FAO and Dr. Mohammad Abdul

Mannan, National Food Utilization and Nutrition Advisor, NFPCSP-FAO, FAO

Representation in Bangladesh for their constant technical support, valuable criticism

and discussion and important suggestions in completion of this work. Additionally,

appreciation is given to Dr. Rezaul Karim Talukder, Socioeconomist, NFPCSP - FAO

for his initial suggestions in the study. Thanks are also due to Dr. Nur Ahamed

Khondaker, Research Grant Administrator, FAO-NFPCSP, FAO Representation in

Bangladesh, for his unswerving administrative support in the completion of this work.

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We are also appreciative of the Finance Department, FAO Bangladesh for its active help

in the smooth release of funds.

We are grateful to Mr. Ciro Fiorillo, Chief Technical Adviser, NFPCSP - FAO, for his

technical and administrative support in carrying out this work.

A final thanks to the Food and Agriculture Organization of the United Nations (FAO) and

the Food Planning and Monitoring Unit (FPMU), Ministry of Food and Disaster

Management for their support under the National Food Policy Capacity Strengthening

Programme (NFPCSP) with financial assistance from EU and USAID.

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RESEARCH TEAM

Principal Investigator Professor Sheikh Nazrul Islam1, PhD Co-Investigators Professor Md. Nazrul Islam Khan1, PhD Professor M. Akhtaruzzaman1, PhD Professor Saiful Huque1, PhD Professor Monira Ahan2, PhD

Laboratory Team Muhammad Ali Siddique3, PhD (BRRI, Gazipu) Ashrafi Hossain4 MSc, (SBAU)

Md. Abdul Jalil5, MSc, PhD student (DAE) Shah Md. Anayet Ullah Siddiqui1, MSc (INFS, DU) Maksuda Khatun6, PhD student (Botany, DU)

Mahbuba Kawser1, MS, M.Phil, PhD student (INFS, DU) Parveen Begum1, MS, M.Phil (INFS, DU) Anjan Kumar Roy7, MSc, M.Phil. student (INFS, DU; ICDDR’B) Sabnam Mustafa1, M. Phil student (INF, DU) Kohinur Begum, MSc (INFS, DU) Abu Bakar Siddique, MSc (INFS, DU Md. Tariqual Islam Sajib4, MSc (SBAU) Dipa Jamal, MSc (INFS, DU)

Tanjina Rahman1, MS (INFS, DU) Farzana Bhuyan1 MSc student (INFS, DU) Mia Sakib Anam1 MSc student (INFS, DU) Syeda Munia Haque, MSc student (INFS, DU)

CFCS Team Nur Mohammad Siddiki, MSc (supervisor) Shafiqul Islam Khan, MSc Rupesh Chakma, MSc Pintu Chakma, MSc Ripan Chakma, MSc

Consultant Professor Sagarmay Barua, PhD (Director, INFS, DU)

1Institute of Nutrition and Food Science, University of Dhaka, Dhaka-1000, Bangladesh; 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh; 3Grain Quality and Nutrition Division, Bangladesh Rice Research Institute (BRRI), Gazipur-1701, Bangladesh; 4Department of Biochemistry, Sher-e-Bangla Agriculture University, Dhaka-1207, Bangladesh; 5Department of Agricultural Extension, Khamar Bari, Dhaka-1215, Bangladesh; Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh; 7Nutrition Biochemistry Laboratory, ICDDR’B, Mohakhali, Dhaka-1212, Bangladesh