a compendium of technical programme (2019-20)

A Compendium

of

TECHNICAL PROGRAMME

(2019-20)

ICAR-Indian Institute of Oilseeds Research

Rajendranagar, Hyderabad-500 030

RESEARCH PROJECTS

PREFACE

This compendium of research projects contains the technicalprogrammes of all the projects to be undertaken at ICAR-IIOR, Hyderabadduring 2019-20. In addition to the above, the technical programme ofexternally funded projects have also been listed. All the technical programmehave been finalized in the Institute Research Council (IRC) meetings held atICAR-IIOR, Hyderabad during 22-24 April 2019, 16th May 2019 and 11th

September 2019. Details of individual experiments/items of work have beenclearly indicated to facilitate easy implementation and monitoring.

It is hoped that this handout will help in clear understanding of thetreatment details in different experiments and their correct implementation inthe field/ laboratories. It is expected that each scientist will strictly adhere tothe schedules of work as per the approved plan by IRC.

(A.Vishnuvardhan Reddy)Director

CONTENTS

LIST OF PROJECTS

Project

No.

Title Page No.

Institute Projects 1

Externally Funded Projects 4

101-5 Exploitation of inter and intraspecific genetic resources for

development of agronomically superior inbred lines and populations in

sunflower

5

102-7

Exploitation of safflower genetic resources for development of

superior breeding lines with high oil yield and adaptation to stresses

12

102-8

Development of parental lines with high oil yield and wilt resistance in

safflower

17

102-9

Development of genetic and genomic resources and identification of

genes/markers for agronomic traits in safflower

22

103-12 Exploitation of plant genetic resources for development of superior

inbred lines in castor

24

103-13

Diversification of parental base and development of superior parental

lines in castor

28

103-14 Development of genomic resources and tools for applications in castor

breeding

35

103-15 Optimization of regeneration and transformation protocols to realize

grey mold resistant transgenic castor (Ricinus communis L.)

37

104-12 Agronomic interventions for increasing productivity and resource use

efficiency (nutrient and moisture) of emerging cropping systems

involving oilseeds

40

104-13

Assessing safflower based cropping systems productivity and resource

use efficiency under different land configurations, crop geometry and

IPNM in different Vertisol types and rainfall patterns

42

104-14

Synthesis and evaluation of polymers for seed health and productivity

of oilseed crops

44

104-15 Screening and identification of potential sources of tolerance to abiotic

stresses and improved physiological efficiency in sesame

47

104-16 Development of best management practices for organic soybean-

sesame cropping system

49

104-17 Fabrication of Fe and Zn nanosystems as efficient nutrient sources 51

104-18 Agronomic interventions for enhancing resource use efficiency in

castor based cropping systems

52

104-19 Developing best management practices for sesame under rice-sesame

cropping system

54

105-11 Development of water dispersible granular (WDG) formulation of

Bacillus thuringiensis var. kurstaki for management of Spodoptera

litura

56

105-12 Screening and identification of durable sources of resistance to

diseases of castor and deciphering the associated mechanisms

57

105-14 Screening and identification of dependable sources of resistance to

insect pests of castor and deciphering the associated mechanisms

59

105-15 Screening and identification of durable sources of resistance to biotic

stresses of sesame and deciphering the associated mechanisms

61

105-16 Exploiting the bioefficacy of entomopathogenic nematodes against

Tobacco caterpillar (Spodoptera litura) and Serpentine leaf miner

(Liriomyza trifolii) in oilseed crops

63

Project

No.

Title Page No.

106-2 Production and characterization of protein hydrolysates from safflower

seed and validation of their utility in animal nutrition

64

107-16 ICT mediated knowledge management and dissemination in different

oilseed crops

65

107-17

On-farm demonstrations of improved technologies and impact

assessment of the adoption

67

107-18 Impact assessment of varieties/hybrids of IIOR mandated crops in

varied agro ecological regions of India

69

107-19 Development of models to predict yield responses to climate change in

oilseed crops

71

108-1

Development of stable Cytoplasmic Genetic Male Sterile (CGMS)

system in sesame through wide hybridization

72

108-2 Exploitation of inter and intra specific genetic resources for

development of elite breeding lines in sesame

73

108-3 Development of genetic and genomic resources and identification of

gene/marker for different agronomic traits in sesame

77

109-1 Exploitation of plant genetic resources for development of improved

breeding populations in niger

79

EXTERNALLY FUNDED PROJECTS

Project

No.

Title Page No.

- Mass production of Bacillus thuringiensis (Bt) and Beauveria bassiana,

formulation as oil based suspension concentrates singly and in

combination and field evaluation (ICAR Network Project- AMAAS)

80

- Development of practicable technologies for field level exploitation of

endophytic Trichodermaas ameliorators of biotic and abiotic stresses in

crops (ICAR Network Project- AMAAS)

82

- Proactive mitigation of gray mold (Botryotinia ricini) disease of castor

(Ricinus communis L.) crop in Telangana State using dynamical disease

forecast (DST – SSTP)

85

- Mapping of QTLs associated with resistance to aphid (Uroleucon

compositae Theobald) in safflower (Carthamus tinctorius L.) using

genome‐wide SNP markers (DST)

87

- Frontline Demonstrations (FLDs) on Oilseeds and other extension

activities (NMOOP, DAC&FW)

88

- Central Sector Scheme For Protection of Plant Varieties and Farmers

Rights Authority (ANNUAL) (PPV&FRA)

90

Seed Production in Agricultural Crops (ICAR)

91

LIST OF SCIENTISTS

Dr. A. Vishnuvardhan Reddy, Director

CROP IMPROVEMENT

1 Dr. M. Sujatha Principal Scientist (Genetics & Cytogenetics)

2 Dr. K. Anjani Principal Scientist (Plant Breeding)

3 Dr. V. Dinesh Kumar Principal Scientist (Biotechnology)

4 Dr. N. Mukta Principal Scientist (Economic Botany)

5 Dr. C. Lavanya Principal Scientist (Plant Breeding)

6 Dr. Senthilvel Senapathy Principal Scientist (Plant Breeding)

7 Dr. Kadirvel Palchamy Principal Scientist (Genetics)

8 Dr. T. Manjunatha Senior Scientist (Plant Breeding)

9 Dr. Mangesh Y. Dudhe Senior Scientist (Plant Breeding)

10 Dr. K. T. Ramya Scientist (Genetics & Plant Breeding)

11 Mrs. B. Usha Kiran Scientist (Biotechnology)

12 Dr. H.H. Kumaraswamy Scientist (Biotechnology)

13 Dr. H.P. Meena Scientist (Plant Breeding)

14 Dr. J. Jawahar Lal Scientist (Plant Breeding)

15 Dr. H.D. Pushpa Scientist (Genetics & Plant Breeding)

CROP PRODUCTION 1 Dr. S.N. Sudhakara Babu Principal Scientist (Agronomy)

2 Dr. P. Lakshmamma Principal Scientist (Plant Physiology)

3 Dr. Lakshmi Prayaga Principal Scientist (Plant Physiology)

4 Dr. G. Suresh Principal Scientist (Agronomy)

5 Dr. P. Padmavathi Principal Scientist (Agronomy)

6 Dr. Md. A. Aziz Qureshi Principal Scientist (Soil Science)

7 Dr. K. Ramesh Principal Scientist (Agronomy)

8 Dr. Ratna Kumar Pasala Senior Scientist (Plant Physiology)

9 Dr. Praduman Yadav Scientist (Biochemistry)

10 Mrs. K.S.V. Poorna Chandrika Scientist (Agrl. Chemicals)

CROP PROTECTION 1 Dr. P.S. Vimala Devi Principal Scientist (Agrl. Entomology)

2 Dr. R.D. Prasad Principal Scientist (Plant Pathology)

3 Dr. M. Santha Lakshmi Prasad Principal Scientist (Plant Pathology)

4 Dr. P.S. Srinivas Principal Scientist (Agrl. Entomology)

5 Dr. P. Duraimurugan Senior Scientist (Agrl. Entomology)

6 Mrs. B. Gayatri Scientist (Nematology)

SOCIAL SCIENCES 1 Dr. S.V. Ramana Rao Principal Scientist (Agrl. Economics)

2 Dr. Ch. Sarada Principal Scientist (Agrl. Statistics)

3 Dr. G.D. Satish Kumar Principal Scientist (Agrl. Extension)

4 Dr. K. Alivelu Principal Scientist (Agrl. Statistics)

5 Mrs. P. Madhuri Scientist (Computer Applications)

1

LIST OF PROJECTS

INSTITUTE PROJECTS

Sl.

No

Project No. Project Name Investigators Date of

start

Propo-

sed date

of com-

pletion

1 101-5 IXX12584

Exploitation of inter and intraspecific genetic resources for development of agronomically superior inbred lines and populations in sunflower

Dr. M. Sujatha Dr. H.P. Meena Dr. H.D. Pushpa Dr. M.Y. Dudhe Dr. Lakshmi Prayaga Dr. R.D. Prasad Dr. P. S. Srinivas Dr. K. Alivelu Dr.A.Vishnuvardhan Reddy

2015

2020

2 102-7 IXX12633

Exploitation of safflower genetic resources for development of superior breeding lines with high oil yield and adaptation to stresses

Dr. P. Kadirvel Dr. N. Mukta Dr. M.A.A. Qureshi Dr. R.D. Prasad Dr. P.S. Srinivas Dr. Praduman Yadav

2015 2020

3 102-8 IXX12568

Development of parental lines with high oil yield and wilt resistance in safflower

Dr. K. Anjani Dr. R.D. Prasad

2015 2020

4 102-9 IXX12571

Development of genetic and genomic resources & identification of genes/ markers for agronomic traits in safflower

Mrs. B. Usha Kiran Dr. V. Dinesh Kumar Dr. P. Kadirvel

2015 2020

5 103-12 IXX12565

Exploitation of plant genetic resources for development of superior inbred lines in castor

Dr. K. Anjani Dr.M.Santhalakshmi Prasad Dr. P. Duraimurugan Dr. Praduman Yadav Dr. P. Lakashmamma Dr. J. Jawahar Lal

2015

2020

6 103-13 IXX12629

Diversification of parental base and development of superior parental lines in castor

Dr. T. Manjunatha Dr. C. Lavanya Dr. S. Senthilvel Dr. P. Lakshmamma

2015

2021

7 103-14 IXX13518

Development of genomic resources and tools for applications in castor breeding

Dr. S. Senthilvel Dr. R. D. Prasad Dr. M. Santha Lakshmi Prasad Mrs. B. Gayathri

2017 2022

8 103-15 IXX13580

Optimization of regenera-tion and transformation protocols to realize grey mold resistant transgenic castor(Ricinus communis L.)

Dr. V. Dinesh Kumar Dr. M. Sujatha Mrs. B. Usha Kiran Dr. H.H. Kumaraswamy Dr. Rohini Sreevathsa, (NRCPB,IARI, New Delhi)

2017 2020

9 104-12 IXX12625

Agronomic interventions for increasing productivity and resource use efficiency (nutrient and moisture) of emerging cropping systems involving oilseeds

Dr. S.N. Sudhakara Babu Dr. Md. Aziz A Qureshi Dr. K. Alivelu

2015

2020

2

Sl. No


start Propo-

sed date of com-pletion

10 104-13 IXX12569

Assessing safflower based cropping systems produc-tivity and resource use efficiency under different land configurations, crop geometry and IPNM in different Vertisol types and rainfall patterns

Dr. P. Padmavathi Dr. M.A. Aziz Qureshi Dr. P. Ratna Kumar Dr. K. Alivelu

2015 2021

11 104-14 IXX13048

Synthesis and evaluation of polymers for seed health and productivity of oilseed crops

Mrs. K.S.V.P. Chandrika Dr. Praduman Yadav Dr. R.D. Prasad Dr. S.N. Sudhakara Babu Dr. P.Padmavathi Dr. Lakshmi Prayaga Dr. P. Ratna Kumar Dr. Anupama Singh (IARI, New Delhi) Dr.Tushar Jana (UoH, Hyd)

2016 2019

12 104-15 IXX13052

Screening and identification of potential sources of tolerance to abiotic stresses and improved physiological efficiency in sesame

Dr. P. Ratna Kumar Dr. J. Jawahar Lal Dr. Praduman Yadav

2016

2019

13 104-16 IXX14616

Developing best management practices for organic soybean-sesame cropping system

Dr. K. Ramesh Dr. M.A.A. Qureshi Dr. P. Duraimurugan Dr. Praduman Yadav

2018 2021

14 104-17 IXX14327

Fabrication of Fe and Zn nanosystems as efficient nutrient sources

Mrs. K.S.V.P. Chandrika Dr. M.A.A. Qureshi Dr. Praduman Yadav Dr. Balaji Gopalan (BITS, Hyderabad) Dr. Anupama Singh (ICAR-IARI, New Delhi)

2018 2022

15 104-18 IXX14476

Agronomic interventions for enhancing resource use efficiency in castor based cropping systems

Dr. G. Suresh Dr. M.A.A. Qureshi

2018 2023

16 104-19 IXX15264

Developing best management practices for sesame under rice-sesame cropping system

Dr. K. Ramesh Dr. M.A.A. Qureshi Dr. Praduman Yadav Dr. Harisudan (Vridhachalam, TNAU) Dr. Jogi Naidu (Amdalavalasa, ANGRAU) Mishra (Mahisapet, OUAT)

2019 2022

17 105-11 IXX12566

Development of water dispersible granular (WDG) formulation of Bacillus thuringiensis var. kurstaki for management of Spodoptera litura

Dr. P. S. Vimala Devi Dr. P. Duraimurugan Mrs. K.S.V.P. Chandrika

2015

2020

18 105-12 IXX12570

Screening and identifica-tion of durable sources of resistance to diseases of castor and deciphering the associated mechanisms

Dr. M. Santha Lakshmi Prasad Mrs. B. Gayatri Dr. Praduman Yadav

2015

2020

3

Sl. No


start

Propo-

sed date

of com-

pletion

19 105-14 IXX14329

Screening & identification of dependable sources of resistance to insect pests of castor & deciphering the associated mechanisms

Dr. P. Duraimurugan 2017 2022

20 105-15 IXX13577

Screening & identification of dependable/durable sources of resistance to biotic stresses of sesame and deciphering the associated mechanisms

Dr. M. Santha Lakshmi Prasad Dr. P. Duraimurugan Dr. H. H. Kumaraswamy

2017 2022

21 105-16 IXX13582

Exploiting the bioefficacy of entomopathogenic nematodes against Tobacco caterpillar (Spodoptera litura) and Serpentine leaf miner (Liriomyza trifolii) in oilseed crops

Mrs. B. Gayatri Dr. P. Duraimurugan Dr. Sunanda Patil (NIPHM, Hyderabad)

2017 2020

22 106-2 IXX13051

Production & characteri-zation of protein hydroly-sates from safflower seed and validation of their utility in animal nutrition

Dr. Praduman Yadav Dr. K.S.V.P. Chandrika Dr. S.V. Rama Rao (ICAR-DOP) Dr. M. Santha Laxmi Prasad

2016 2019

23 107-16 IXX12572

ICT mediated knowledge management and dissemination in different oilseed crops

Mrs. P. Madhuri Dr. Ch. Sarada Dr. N. Mukta Dr. G.D. Satish Kumar Dr. P.Padmavathi Dr. S.V. Ramana Rao Dr. G. Raghunath Dr. K. Anjani Dr. S.N. SudhakaraBabu Dr. P. Kadirvel Mrs. B. UshaKiran Dr. P. Satya Srinivas Dr. R.D.Prasad Dr. Praduman Yadav Dr. H.P. Meena

2015

2020

24 107-17 IXX13053

On-farm demonstrations of improved technologies and impact assessment of the adoption

Dr. S.V. Ramana Rao Dr. G.D. Satish Kumar Dr. S.N. Sudhakara Babu Mrs. P. Madhuri

2016 2020

25 107-18 IXX13581

Impact assessment of varieties/hybrids of IIOR mandated crops in varied agro ecological regions of India

Dr. S.V. Ramana Rao Dr. C. Sarada

2017 2022

26 107-19 IXX14482

Development of models to predict yield responses to climate change in oilseed crops

Dr. K. Alivelu Dr. C. Sarada

2018 2021

27 108-1 IXX10783

Development of stable cytoplasmic genetic male sterile system in sesame through wide hybridization

Dr. Jawahar Lal J. 2013

2019

4

Sl. No


start

Propo-

sed date

of com-

pletion

28 108-2 IXX13579

Exploitation of inter & intra specific genetic resources for development of elite breeding lines in sesame

Dr. K.T. Ramya Dr. Jawahar Lal J.

2017 2022

29 108-3 IXX13870

Development of genetic and genomic resources and identification of gene/ marker for different agronomic traits in sesame

Dr. H.H. Kumaraswamy Dr. M. Santha Lakshmi Prasad Dr. P. Duraimurugan Dr. P. Ratna Kumar

2017 2020

30 109-1 IXX13629

Exploitation of plant gene-tic resources for develop-ment of improved breed-ing populations in niger (Guizotia abyssinica Cass.)

Dr. H.D. Pushpa Dr. A. Vishnuvardhan Reddy

2017 2022

EXTERNALLY FUNDED PROJECTS Sl. No

Project Name Investigators Date of

start

Date of com

pletion

Sponsor-ing orga-nization

1 Mass production of Bacillus thuringiensis (Bt) and Beauveria bassiana, formulation as oil based suspension concentrates singly & in combination and field evaluation

Dr. P.S. Vimala Devi

Dr. P. Duraimurugan

Mrs. K.S.V.P.

Chandrika

2017 2020 ICAR

Network-

AMAAS

2 Development of practicable technologies for field level exploita-tion of consortia of microbial agents as ameliorators of biotic and abiotic stresses in crops

Dr. R.D. Prasad Dr. P. Lakshmamma Dr. Md. A. Aziz Qureshi Mrs. K.S.V. P. Chandrika

2017 2020 ICAR Network-AMAAS

3 Proactive mitigation of gray mold (Botryotinia ricini) disease of castor (Ricinus communis L.) crop in Telangana State using dynamical disease forecast

Dr. R. D. Prasad Dr. Ch. Sarada

2016 2019 DST-SSTP

4 Mapping of QTLs associated with resistance to aphid (Uroleucon compositae Theobald) in safflower (Carthamus tinctorius L.) using genome‐wide SNP markers (DST)

Dr. P. Kadirvel Dr. P.S. Srinivas

Dr. S. Senthilvel

2017

2020

DST

5 Frontline Demonstrations (FLDs) on Oilseeds and other extension activities

Dr. G.D. Satish Kumar Dr. S.V. Ramana Rao Dr. C. Sarada Dr. P.S. Srinivas Dr. R.D. Prasad

2014 2020 NMOOP of DAC&FW, GOI

6 Central Sector Scheme For Protection of Plant Varieties and Farmers Rights Authority (ANNUAL) (PPV&FRA)

Dr. N. Mukta Dr. C. Lavanya Dr. M.Y. Dudhe

2003 -

7 Seed Production in Agricultural

Crops

Dr. S.N. Sudhakara Babu Dr. T. Manjunatha Dr. C. Lavanya Dr. J. Jawahar Lal Dr. H.P. Meena

2014

- ICAR

Network

5

INSTITUTE PROEJCTS

Project No. 101-5

Title Exploitation of inter and intraspecific genetic resources for

development of agronomically superior inbred lines and

populations in sunflower

Principal Investigator M. Sujatha

Co-Investigators H.P. Meena

M.Y. Dudhe

H.D. Pushpa

Lakshmi Prayaga

R.D. Prasad

P. Satya Srinivas

K. Alivelu

A. Vishnuvardhan Reddy

PIMS Number IXX12584

Year of start 2015

Objectives 1. Development of agronomically superior inbreds and parental

lines for early maturity, high oil and high seed yield

2. Development of high seed/oil yielding hybrids suited for

different conditions

3. Identification of physiologically efficient lines and inbreds

tolerant to temperature and water logging

4. Confirmation of trait specific germplasm and inbreds for

resistance to Alternariaster helianthi, SND and powdery mildew

and development of inbreds for disease resistance

5. Incorporation of leaf hopper resistance in promising inbreds

Experiments/Activities (2019-20)

Objective 1: Development of agronomically superior inbreds and parental lines for early

maturity, high oil and high seed yield

Scientist(s) Activity-1 Deliverable(s)

M.Y. Dudhe Seed multiplication of germplasm accessions 100 g seed of each accession

Methodology • Entries : 90

• Season : Late kharif (August end)

• Plot size : 2 rows of 4 m length

• Land required : 400 m2

• Farm : Narkhoda


M.Y. Dudhe

M. Sujatha

Seed multiplication of USDA accessions for

supply and deposition in MTS and LTS in

NBPGR

100 g seed of each

accession

Methodology

• Entries : 690 accessions (300 breeding lines and inbreds and 390 newly

augmented accessions)

• Season : Kharif and Rabi


• Farm : Rajendranagar and Narkhoda in staggered sowings of 150 accessions each

6


M.Y. Dudhe Multilocation evaluation of promising accessions

for high yield and oil content

Identification of

promising inbreds

Methodology

• Entries : 32 (18 USDA &15 GMU accessions)


• Replication : 2

• Season : Late kharif (August end)

• Locations : Akola, Latur, Nandyal , Bengaluru, Coimbatore, Tornala &

Hyderabad


M. Sujatha Multiplication, evaluation, distribution,

and maintenance of the CMS lines

repository

Supply to the breeders and

MTS/LTS at IIOR and

NBPGR, respectively

Materials

• Treatments : 120 A/B lines

Methodology

• Sowing will be taken up in 2 sets

• Plot size : A line 3 rows and B line 2 rows of 4.5 m

• Spacing : 60 x 30 cm

• Design : Augmented block

• Area : 2200 m2

• Season : Kharif

• Farm : Rajendranagar


M. Sujatha Multiplication of promising trait specific

lines

To be used as checks

and for distribution

Materials

Treatments: 24 inbreds (PM-81 ,PS-2023 , HA-124A/B, TSG-208 , GP-4-1424,

TSG-339, CMS-300 A/B, TSG-207, TSG-24, HAR-9, TSG-212,

TSG-401, TSG-217, GMU-339, GMU-25 ,HAR-9 , TSG-17, TSG-

355, TSG-292 (RHA 348), TSG-411, GMU-689, GMU-804, LTRR-

341, TSG-332)

Methodology

• Plot size : 4 rows of 4.5 m


• Area : 450 m2

• Season : Kharif


H.P. Meena

M. Sujatha (for H.

praecox combination)

Characterisation, evaluation and generation

advancement of prebred material from

BC2F2 to BC2F3 and BC2F3 to BC2F4

Promising prebred

material

Materials • Number of progenies: 800 (15 combinations)

7

Methodology • Plot size : 2 rows of each progeny of 4.0 m


• Design : ABD

• Season : II FN of June

• Area : 6000 m2

• Farm : Rajendranagar (for multiplication, generation

advancement and validation of markers being

identified) and Narkhoda (evaluation and generation

and selection)


H.P. Meena Evaluation and utilization of restorer gene

pool progenies

Promising breeding

material

Materials • Treatments : 200 progenies + 4 checks (RHA-6D-1, RHA-1-1, RHA-95C-1,

P-62R)

Methodology • Plot Size : 2 rows of 4.0 m

• Spacing : 60 cm x 30 cm

• Date of sowing : IIFN of July

• Area : 1200 m2

• Location : Narkhoda


Pushpa H.D. Evaluation and utilization of maintainer

gene pool progenies

Promising breeding

material

Materials • Treatments : 40 progenies + 4 checks (CMS-17B, CMS-234B, ARM-243B,

CMS-7-1B)

Methodology • Plot Size : 2 rows of 4.0 m

• Spacing : 60 cm x 30 cm

• Date of sowing : Ist FN of July 2019

• Area : 250m2

• Location : Narkhoda

Statistical analysis of yield data with qualitative and quantitative characters

Scientist(s) Activity Deliverable(s)

K. Alivelu

To assess the extent of association between important

agronomic characters and yield.

Correlation studies

To estimate heritability and genetic advance among

Inbred lines for seed yield and component traits.

Useful for selection of

lines by the breeder

To partition the association of component characters

with seed yield in to direct and indirect effects using

path coefficient analysis.

Direct and indirect

effects on seed yield

To estimate the genetic divergence among Inbred

lines.

Classification of

Inbred lines to know

the extent of variability

8

Objective 2: Development of high seed/oil yielding hybrids suited for different

conditions


H.P. Meena Seed multiplication of seven entries for

IHT & AHT trials

Promising hybrids

Materials Number of entries: 7 hybrids [IIOSH-15-10(AHT-II), IOSH-15-20(AHT-I), IOSH-460(IHT)

IOSH-413 (IHT), IOSH-566 (IHT), IOSH-434(IHT) & IOSH-572(IHT) Methodology

• Plot size : 60 rows of A lines and 15 rows of R line of each hybrid of 4.0m


• Design : ABD

• Season : Late kharif (3 entries) &Rabi (4 entries)

• Area : 2800 m2

• Farm : Narkhoda Scientist(s) Activity-2 Deliverable(s)

H.P. Meena, M. Sujatha,

M.Y. Dudhe,

Pushpa H.D

Generation of new experimental

hybrids

Identification of promising

heterotic combinations and

development of new hybrids Materials • 40 inbreds from new prebred lines to be crossed with 2 CMS lines viz.17 A & ARM 243A

• 20 promising inbreds from IIOR and AICRP with 4 CMS lines (COSF-6 A, COSF-7A,

CMS-103A and ARM-243A)

• 30 promising R lines from USDA, R gene pool with 4 CMS lines (CMS 1001A,

CMS1008A, ARM 243A, CMS 234A) and 6 most promising R lines with 60 CMS lines Methodology

• Plot size : 10 rows of each CMS and 2 rows of R lines in two staggering


• Design : ABD

• Area : 1600 m2

• Season : II FN of August (Set 1) at Rajendranagar Farm; IIFN of

September (Set II) at Narkhoda farm


H.P. Meena, M. Sujatha

M.Y. Dudhe, Pushpa H.D.

A. Vishnuvardhan Reddy

Evaluation of preliminary

hybrids

Identification of promising

cross combinations

Materials

• Number of hybrids : 600 + 2 checks (DRSH-1 & KBSH-44) in 3 different sets

Methodology • Plot size : 2 rows of 4.0 m


• Design : Augmented

• Area : 4000 m2

• Date of sowing: 1st fortnight of August

• Farm : Narkhoda Observations

Days to maturity, plant height (cm), head diameter (cm), 100-seed weight (g), oil

content (%), seed yield/plant (g) etc.

9


H.P. Meena Nucleus seed production of DRSH-

1 parental lines

Good quality nucleus seed

supply to BSP Materials

• Treatments : ARM-243A & B and RHA-6D-1 Methodology

• Plot size : A (40 rows), B (15 rows) and R (30 rows) of 4.0 m


• Sowing : 1st fortnight of October

• Area : 250 m2

• Farm : Narkhoda Objective 3: Identification of physiologically efficient lines and inbreds tolerant to

temperature and drought and salinity or abiotic stresses


Lakshmi Prayaga Evaluation of sunflower inbreds for

temperature and combined stress of

drought + temperature tolerance

Set of improved breeding

lines for drought and

temperature tolerant traits Material: 40 - 50 lines (CMS & R) Methodology

• Staggered sowings

• Replications: 3

• Plot size: 0.6 x 4.5 m

• Design: strip-split plot

• Observations: RWC, SPAD, leaf temperature, soil moisture, yield and yield

attributes, weather parameters


Lakshmi Prayaga Understanding the mechanism of

temperature tolerance

Temperature tolerance

mechanism Material: 8 contrasting lines (4+4) from preliminary study Methodology


• Replications: 3

• Plot size: 3 x 4.5 m

• Design: split –plot

• Observations: antioxidant enzyme activity, RWC, membrane stability, leaf

temperature, wax content, pollen fertility, growth rates, yield and yield attributes


Lakshmi Prayaga Effect of temperature on root growth

(polybags)

Adds to understanding the

mechanism of tolerance

Material: 8 contrasting lines (4+4) Methodology


• Replications: 3 (one replication=10 plants)

• Design: split plot

• Observations: root length, root fresh weight, root dry weight, root length density, root

volume, TDM, leaf appearance rate, leaf area at flowering, leaf temperature

10


Lakshmi Prayaga Evaluation of sunflower inbreds for salinity

tolerance at Gangavathi or Machilipatnam

Same set used for tempera-

ture and drought tolerance

Material: 40 - 50 lines (CMS & R)

Methodology

• Replications: 3

• Plot size: 1.2 x 4.5 m

• Design: RBD

• Observations: Germination and survival %, growth characters, yield and yield

attributes

Objective 4: Confirmation of trait specific germplasm and inbreds for resistance to

Alternaria ster helianthi, SND and powdery mildew and development of

inbreds for disease resistance

Scientist(s) Activity Deliverable

R. D. Prasad Screening of BC2F

3 families (about 100)

of ARM 243 x H. praecox against powdery mildew

Identification of resistant material for exploitation in the breeding programmes

Materials

100 F3 families of ARM 243 x H. praecox

Resistant check: PM-81 (RC); Susceptible check: 2023B

Methodology

Scoring ‘disease severity’ of individual plants grown in field raised under natural

infection

Plot size: 2 rows of 4.5 m

Spacing: 60 x 30 cm

Season: Rabi

Farm: Rajendranagar

Objective 5: Incorporation of leafhopper resistance in promising inbreds

1. Symptom mapping of leafhoppers on sunflower

Scientist associated Activity-1 Deliverable(s)

P. S. Srinivas Confirmation of Symptom mapping for

leafhoppers

Differentiation of leafhopper

symptoms from senescence Treatments/materials :

4 susceptible accessions – TSG-295, PSCIM- 186, PSMO-53B-1; NDCMS- 2B

4 resistant – TSG- 197, TSG- 401, GMU- 504, TSG-403

Methodology

Design : RBD

Replications : 3

Regimes : Protected and un protected

Season : rabi/summer

Observations : Leafhopper population and symptom development will be

documented at regular interval

2. Identification of a new susceptible check to leafhoppers in sunflower


P. S. Srinivas Identification of a new susceptible

check to leafhoppers in sunflower

confirmation of susceptible check

to replace existing Morden

11

Treatments/materials: Seven varieties/inbreds (DRSF-108, DRSF-113, HA 2023A,

HA2023B, NDCMS-2A NDCMS-2B, CMS-125A) + susceptible

check, Morden

Methodology

Replications : 3 (4m row length)

Spacing : 60x 30cm

Season : Summer

Date of sowing : Third week of January

Sandwich method: Sowing morden/NDCMS-2B and test entries, 1:2 to ensure

leafhopper population

Observations : Jassid population at regular interval, Injury rating

Farm : Rajendranagar

3. Identification of resistant inbreds, CMS (B/R) line/ wild accessions of sunflower to

leafhoppers


P. S. Srinivas Identification of resistant inbreds, CMS(B/R) line/

wild accessions of sunflower to leafhoppers

Confirmation of resistant

sources of sunflower to

leaf hoppers

Treatments/materials :

20 accessions (TSG- 391, TSG-403, RCR-CMS-104A, CMS-30A, +16 stable lines

derived from H. argophyllus susceptible check, Morden, NDCMS-2B

Methodology

Spacing : 60x 30cm

Season : Summer

Replications : 2 (4m row length)

Date of sowing : Third week of January

Sandwich method: Sowing susceptible and test entries, 1:2

Observations : Injury rating


4. Association of biochemical components of sunflower with resistance to leafhoppers


P. S. Srinivas Association of biochemical components of

sunflower with resistance to leafhoppers

Major biochemical components

associated with leafhopper

resistance is known

Treatments/materials: 7 Resistant: GMU-339, GMU-504, TSG-403, TSG-349,AKSFI-46-2

TSG-197, and TSG HA 430B

7 Susceptible:PSCRM 127, PSCIM-186, PSMO-53-B-1, TSG-355,

NDCMS-2B, sPSERM-139, Morden,

Methodology

• Design : RBD

• Replications : 1(4m row length)

• Season : Summer

• Methodology : Protected and un-protected

• Observations : Leafhopper population and injury rating

• Plant analysis : Phenols, non-reducing sugars, proteins, free amino acids at

flowering stage

12

Objectives

1. To augment and maintain safflower genetic resources

2. To identify reliable germplasm sources for high seed yield related traits, oil content

and tolerance to stresses

3. To develop breeding lines with high oil yield potential

Experiments/activities (objective-wise) Objective 1: To augment and maintain safflower genetic resources

Scientist(s) Activity 1 Deliverable(s)

N. Mukta

Praduman Yadav

Characterization, evaluation and

multiplication of fresh collections

Identification of diverse

trait specific accessions Materials

• 159 accessions/selections from USDA & SAARC for multiplication/evaluation + 4

checks (A-1, PBNS-12, NARI-57, Bhima) Methods

• Design : ARBD


• Plot size : 1 row x 6m

• Season : Rabi

• Date of sowing : October 2019

• Farm : ICRISAT

• Land area required : 800m2

Observations

• Seed yield components and Oil content (%)


N. Mukta Viability testing,

rejuvenation,

multiplication,

conservation and

supply

Maintenance of seed quality, fresh seed

production of germplasm accessions and supply

Conservation of 258 accessions in cold storage

facility

Utilization through supply Materials

• Rejuvenation of 447 accessions under nets

• Viability testing of ~500 accessions, rejuvenation of accessions with germination

<85%

• Multiplication of promising accessions: 100

• Conservation of 258 accessions after packing in triple layered aluminum pouches

Project No. 102-7

Title Exploitation of safflower genetic resources for

development of superior breeding lines with high oil

yield and adaptation to stresses

Principal Investigator P. Kadirvel

Co-Investigator(s) N. Mukta

M.A. A. Qureshi

R.D. Prasad

P.S. Srinivas

Praduman Yadav

PIMS No. IXX12633

Year of start 2015

13

Methods

• Design : ARBD


• Plot size : 1 row x 6 m

• Season : Rabi


• Farm : ICRISAT

• Land area required : 4500 m2

Objective 2: To identify reliable germplasm sources for high seed yield related traits, oil

content and tolerance to stresses


N. Mukta

Praduman Yadav

Confirmation of germplasm for seed and

oil yield

Promising germplasm for

seed yield and oil content

Materials

• 27 accessions selected for confirmation based on evaluation during 2017-18 and

2018-19 [GMU-2284, GMU-2709, GMU-2745,GMU-3074, GMU-3379, GMU-3471,

GMU-3772, GMU-3974, GMU-7862,GMU-7869, GMU-7871,GMU-7879, GMU-

7880,GMU-7881, GMU-7882, GMU-7884, GMU-7887, GMU-7898, GMU-7899,

GMU-7901, GMU-7905, GMU-7907,GMU-7917, GMU-7920, GMU-7922, GMU-

7923, EC 863182-2] + 5 checks (A-1, PBNS-12, NARI-57, Bhima, JSI-99)

Methods

• Design : Randomized block

• Replication : 2

• Plot size : 3 rows x 5 m


• Season : Rabi


• Farm : ICRISAT


Observations

• Seed yield components and Oil content (%)


M.A.A. Qureshi

Evaluation of selected genotypes for salt

tolerance in laboratory

Salt tolerant genotypes

confirmed

Materials

• 7 genotypes (IC-406143, EC-661173, IC-406052, IC-338209, PBNS-12, A-1,

SSF-733) + New accessions from USDA (GMU-7926)

Methodology

• Paper towel experiment

• Irrigation water: 1) Normal Hoagland's solution 2) Hoagland's with EC level

(10 dS/m)

Observations

• Germination percentage

• Root length

• Shoot length

14

• Dry matter

• Shoot Na, K content

• Root Na, K content

• Shoot Na/K ratio


M.A.A. Qureshi Evaluation of selected genotypes for salt

tolerance in greenhouse

Salt tolerant genotypes

confirmed

Materials

• 11 genotypes (GMU-6506, GMU-5075, GMU-3281, IC-406143, EC-661173,

IC-406052, IC-338209, PBNS-12, A-1, SSF-708, SSF-733)

Methodology

• Multi cell tray experiment

• Irrigation water: 1) Normal Hoagland's solution 2) Hoagland's with EC level:

10 dS/m

Observations

• Germination percentage

• Shoot length

• Dry matter

• Shoot Na, K content

• Shoot Na/K ratio


M.A.A. Qureshi Field screening of selected genotypes

against salinity under field conditions

Response of tolerant genotypes

under field condition determined Materials

• 11 genotypes (GMU-6506, GMU-5075, GMU-3281, IC-406143, EC-661173,

IC-406052, IC-338209, PBNS-12, A-1, SSF-708, SSF-733) Methodology

• Design : Randomised block

• Replication : 3

• Plot size : 3 m x 2 m


• Season : Rabi

• Date of sowing :

• Location : ARS, Machilipatnam, ANGRAU [Well drained

black soils (Inceptisols), EC=10-11 dS/m) Observations

• Seed yield parameters Objective 3: To develop breeding lines with high oil yield potential


N. Mukta, P. Kadirvel

Praduman Yadav

Combined evaluation trial for

germplasm and breeding lines

Promising germplasm for

seed yield and oil content

Materials

• 2 accessions (IC 338171, IC 337833) + 2 breeding lines (F5-55, BC2F6-38-9-4) + 5

checks (A-1, PBNS-12, NARI-57, ISF-764, Bhima)

15

Methods

• Design : RBD

• Replication : 2



• Season : Rabi


• Farm : ICRISAT

• Land area required : 1700 m2 Observations

• Seed yield components, Oil content (%) and Fatty acid content (%)


P. Kadirvel

Praduman Yadav

Preliminary evaluation of high oil

selections for seed yield

Potential high oil yielding

genotypes identified Materials

• 43 selections (>35% oil content) + 5 checks (A-1, Bhima, PBNS-12, NARI-57,

EC-736501) Methodology

• Design : Augmented



• Season : Rabi


• Farm : ICRISAT

• Land area required: 800 m2 Observations

• Seed yield/plant

• Oil content (%)


P. Kadirvel

Evaluation of segregating

populations of the cross: MMS-1-3

x A-1 for GMS

Inheritance of GMS in safflower

GMS source MMS-1-3 determined

Materials

• F2 and backcross populations of the cross: MMS-1-3 x A-1 for GMS Methodology

• Individual plants (circa 1000)


• Season : Rabi


• Farm : ICRISAT

• Land area required : 200 m2 Observations

• Morphological observations on floral traits

• Pollen sterility


R.D. Prasad

Screening of promising genotypes for

reaction to Fusarium wilt

Potential high oil yielding

genotypes with Fusarium wilt

resistance identified

16

Materials

• 59 germplasm accessions + 50 breeding lines + 2 checks [Nira (S) and TSF-1 (R)] Methodology

• Laboratory screening: Sick Pot Observations

• Plant stand and disease incidence at 15 days interval


P.S. Srinivas

Screening of promising

genotypes for reaction to aphid

Potential high oil yielding genotypes

with aphid resistance identified Materials

• 9 germplasm accessions + 50 breeding lines + 2 checks (CO-1, EC-523368-2) Methodology

• Plot size : 1 row (2 m)


• Replication : 2

• Season : Rabi

• Date of sowing : 2nd fortnight of Nov. 2019

• Farm : ICRISAT


Observations

• Days to wilt after aphid infestation


P. Kadirvel

N. Mukta

Seed multiplication of promising lines of

IIOR/parental lines and released varieties from NARI

Maintenance

Materials

• 14 breeding lines (IIOR) (F5-55, BC2F6-38-9-4, F5-8, F5-125, F5-157, SAF-20B,

SAF-39A, SAF-60, THS-86-2-1, BC1F6-12-39-3-3, BC2F6-38-1-7, BC2F6-38-14-15,

BC3F4-16-12, BC3F4-16-27

• 10 varieties/parental lines (NARI)

Varieties: NARI-6, NARI-96, NARI-57, Nira

Parental lines:

MMS White (Female parent of NARI-NH-1)

C 2829-5-3a-6 (Male parent of NARI-NH-I)

MSV-10-1-5 (Female parent of NARI-H-15)

GMU-2369 (Male parent of NARI-H-15)

TMS-3-1-9-1 (Female parent of NARI-H-23)

D-152-12 (Male parent of NARI-H-23) Methodology



• Season : Rabi

• Date of sowing : 2nd fortnight of October 2019

• Farm : ICRISAT


17

Project No. DOR-102-8

Title Development of parental lines with high

oil, yield and wilt resistance in safflower

Principal Investigator K. Anjani

Co-Investigator R. D. Prasad

PIMS No. IXX12568

Year of start 2015 Objectives:

1. Developing high combining, high oil, high yielding, and wilt resistant diverse inbred

parental lines for safflower hybrid breeding.

2. Developing safflower hybrids with high seed yield (>14q under rainfed, >20q under

irrigation), oil content (30% & above), and resistance to Fusarium wilt. Whether (RAC/ IRC/XII FYP/ QRT /Vision) followed: RAC/ IRC/XII FYP/ QRT /Vision

Technical Programme Activity 1 Objective 1: Developing high combining, high oil, high yielding, and wilt resistant diverse

inbred parental lines for safflower hybrid breeding.

Scientist

associated

Activity-1 Deliverable(s)

K. Anjani

Development of high oil,

high yielding, and wilt

resistant diverse inbred

parental lines.

High oil (35% and above) inbred lines

High yielding (>10 q/ha) inbred lines

High yielding short duration (DF:60-65 days)

inbred lines

High yielding wilt resistant inbred lines

Alternaria tolerant and aphid inbred lines

R.D. Prasad Screening of breeding

lines against wilt in wilt

sick plot

No programme*

*Screening of breeding material at ICAR-IIOR wilt sick plot was over A. Development of Parental/Inbred Lines/Varieties

1. Generation advancement

a. Generation advancement of selections from RIPE population

i) S1 generation of selections from 4th cycle RIPE population

No. of selections: 61

ii) S2generation of selections from 3rd cycle RIPE population


iii) S3generation of selections from 2nd cycle RIPE population


iv) S4generation of selections from 1st cycle RIPE population


Plot size: Plot size: 222.5 sq.m Spacing: 45 x 20 cm Farm: IIOR-ICRISAT

18

b. Advancement of F6 generation

i) NCP -High oil

No. of F6 families: 6

ii) High oil

No. of F6 families: 5

c. Advancement of F3 generation


d. Advancement of F2 generation


e. Advancement of BC2F1


Checks: A1, PBNS-12 spacing: 45 x 20 cm plot size: 236.25sq.m Farm: IIOR-ICRISAT

2. Multiplication of high yielding varieties

1 ISF-309 10 ISF-2035 (nsp)

2 ISF-6974-nsp 11 ISF-53-15(nsp)

3 SEL-153-26(ole) 12 ISF-134-15(nsp)

4 ISF-110-48 (ole) 13 ISF-763(nsp)

5 ISF-317 14 SPP-70(nsp)

6 ISF-319 15 ISF-1

7 ISF-320 16 ISF-2342-17

8 ISF-123-sel-15 17 ISF-2305-17

9 ISF-22-15 18 ISF-2699-17

No. of varieties: 18

Spacing: 45 x 20 cm plot size: 450 sq.m Farm: IIOR-ICRISAT

3. Multiplication of high oil lines

ISF-2106 (O) ISF-3170(O) ISF-2137(O) ISF-1691(O)







ISF-3840 (O) ISF-2069(O) ISF-1825 (O) ISF-156(O)

ISF-473(O) ISF-1301(O) ISF-2078(O) ISF-3793(O)

ISF-2437 (O) ISF-2129(O) ISF-3915(O)

No. of inbred lines: 39

Spacing: 45 x 20 cm plot size: 4.50-6.75 sq.m Farm: IIOR-ICRISAT

4. Multiplication of high oil-high oleic lines

ISF-288(ole) ISF-2846(ole) ISF-802(ole)

ISF-2873(ole) ISF-2893(ole) ISF-2897(ole)

ISF-324 (ole) ISF-1292(ole) ISF-326(ole)


19


ISF-146(ole) ISF-307(ole)






Spacing: 45 x 20 cm plot size: 4.50-6.75sq.m Farm: IIOR-ICRISAT

5. Multiplication of AICRP-trial varieties

Variety Trial

1 ISF-22-15 IVT-2019-20

2 ISF-48-15

3 ISF-123-sel-15

4 ISF-320

5 ISF-87-15 AVT-I-2019-20

6 ISF-116

7 ISF-849-sel-16

8 ISF-112-15 AVT-II-2019-20

9 ISF-1258-15-nsp Promoted variety

10 ISF-2342-17 IVT-2020-21


Spacing: 45 x 20 cm Plot size: 62.75-90 sq.m Farm: IIOR-ICRISAT

6. Maintenance of trait-specific inbred lines

a. Aphid tolerant inbred lines

1 ISF-51-15

2 ISF-161-15

3 R-sel-05-63-4-5-19

4 ISF-1305-sel-15

5 ISF-158-15

6 ISF-121-sel-15

7 ISF-3840-sel-15

b. Wilt differentials

1 96-508-2-90

2 DSF-4

3 DSI-116/ISF-116

4 ISF-28-15

5 DSI-104

6 ISF-2305

7 ISF-2258-17

8 ISF-2471-17

20

c. Fusarium wilt resistant inbred lines

1 ISF-2342 (ISF-2342-17)

2 ISF-2413-17

3 ISF-2471-17

d. Alternaria tolerant inbred lines

1 ISF-1749-1-5-2016/ISF-1475

2 ISF-1703-2-1-2016/ISF-1479


Spacing: 45 x 20 cm Plot size: 4.5 sq.m Farm: IIOR-ICRISAT

7. Multiplication of short duration varieties

1 ISF-867

2 ISF-833

3 JSI-99 (SD-C)

4 A1 (ND-C)

No. of varieties: 2+2 checks

Spacing: 30 x 20 cm Plot size: 15 sq.m Farm: IIOR-ICRISAT

8. Nucleus seed production of varieties

1 ISF-764

2 ISF-1

3 ISF-763

4 SPP-70/ISF-70-nsp

Spacing: 45 x 20 cm Plot size: 67.5-112.5 sq.m Farm: IIOR-ICRISAT

B. Hybrid Development Programme

1.Maintenance of male parental lines

1 ISF-2038-A

2 ISF-2757-sel-15

3 ISF-3313sel

4 ISF-48-15

5 ISF-86-15

6 ISF-2042/ISF-112-15

7 ISF-132-15

8 2997-P11-P7

9 ISF-764

10 ISF-855

11 1705-p22

No. of parents: 11


21

2. Hybrid seed production

Hybrid 1 ISH-413 (IHT-2020-21) Hybrid 7 ISH-428 (IHT-2020-21)

Male parent SFS-2038 Male parent 2997-p11-p7

Female parent A-133-1 Female parent A-133-1

Hybrid 2 ISH-416(IHT-2020-21) Hybrid 8 ISH-423 (IHT-2019-20)

Male parent ISF-2757-sel-15 Male parent ISF-86-15


Hybrid 3 ISH-417(IHT-2020-21) Hybrid 9 ISH-400 (AHT-II-2020-21)

Male parent ISF-3313 sel Male parent ISF-764


Hybrid 4 ISH-419(IHT-2020-21) Hybrid 10 ISH-401 (AHT-II-2019-20)

Male parent ISF-48-15 Male parent ISF-764

Female parent A-133-1 Female parent A-133-II

Hybrid 5 ISH-425(IHT-2020-21) Hybrid 11 ISH-402(AHT-II-2019-20)

Male parent ISF-112-15 Male parent ISF-855


Hybrid 6 ISH-427(IHT-2020-21)

Male parent ISF-132-15

Female parent A-133-1

No. of parents: 22


3. Nucleus seed production of A- & B-lines

1 A-133-I

2 B-133-I

3 A-133-II

4 B-133-II

5 (MGMS-2292 5s x MGMS-2292

2F)-marker –linked GMS line

No. of lines: 5 Spacing: 45 x 20 cm Plot size: 618.75 sq.m. Farm: IIOR-

ICRISAT

C. Conduction of AICRP (Safflower) Breeding Trials

Trial Method No. of entries

IVHT RBD, 3 replications, gross plot size: 2.25 m x 5 m 19+ 3 checks

AVT-I RBD, 3 replications, gross plot size: 11+2 checks

AVHT-II RBD, 3 replications, gross plot size: 2.70 m x 5.0 m 7+ 3 checks

22

Objectives

1. To develop reference genome and transcriptome of safflower through next

generation sequencing (NGS)

2. To develop germplasm/mapping panel for oil content in safflower

3. To identify and validate candidate genes associated with oil content through allele

mining or association mapping

4. To map genes/QTLs associated with key oil yield enhancing traits

Experiments/Activities (Objective-wise)

Objective 3: To identify and validate candidate genes associated with oil content

through allele mining or association mapping


B. Usha Kiran

Identification and validation of candi-

date genes associated with oil content

Genes/alleles contributing for

high oil and hull content

Material

• 20 high and low oil lines of safflower (EC736511, GMU4907, EC736506,

GMU5037, GMU391, EC736499, EC736498, EC755674, GMU3966, GMU3992,

EC736500-1, EC736501-1, EC736495, EC736499, EC736498 GMU1153,

GMU5701, GMU1241, GMU7338, GMU1161)

• Genes: 12 (WRK1, LEC1, LEC2, Fusca transcription factors, Promoter region of

genes DGAT, GPAT, FATA, FATB, KCS, destaurases, PDAT, PDCAT) Methodology

• Genotyping with developed gene specific primers

• Sequencing of PCR product/ cloned genes

Observations

• Identification of the sequence variations of the candidate genes

• Associating the sequence variation to the trait through TASSEL


B. Usha Kiran

V. Dinesh Kumar

Cloning and sequencing of the major

genes involved in oil biosynthesis

Genes/alleles contributing

for high oil and hull content

Material

• 10 safflower germplasm mapping panel lines (GMU1241, GMU5701, GMU1153,

A1, GMU1185, EC736496, EC736497, EC736499, EC736495, EC736501-1)

• Genes: Based on the preliminary results of sequence analysis, only such genes

which show sequence variations (indels) will be selected for cloning purpose.

Both TFs and structural genes will be analysed. As the genes are long, it will

involve cloning fragments for the analyses.

Project No 102-9

Title Development of genetic and genomic resources and identi-

fication of genes/markers for agronomic traits in safflower

Principal Investigator B. Usha Kiran

Co-Investigator(s) V. Dinesh Kumar

P. Kadirvel

PIMS No. IXX12571

Year of start 2015

23

Methodology

• Cloning of the genes with gene cloning kit.

• Sequencing of cloned gene Observations

• Identification of the sequence variations of the candidate genes ,



B. Usha Kiran

Validation of the sequence variations in

candi-date genes associated with oil content

Genes/alleles

contributing for high oil

and hull content Material

• 100 safflower germplasm mapping panel lines and in mapping population Methodology

• Genotyping with developed gene specific primers

• Sequencing of PCR product/ cloned genes Observations

• Identification of the sequence variations of the candidate genes


Objective 4: To map genes/QTLs associated with key oil yield enhancing traits


P. Kadirvel

Marker-trait association analysis in F2

population of the cross: A-1 x EC-755673-1

Putative marker

associated with oil

content validated Material

• F2 population (A-1 x EC-755673-1) (n=300) Methodology

• Genotyping based on putative SSR markers (Agarose gel electrophoresis)

• Phenotyping of F3 seeds Observations

• SSR allelic segregation data

• Oil content (%)


P. Kadirvel

Generation advancement of mapping

populations

F2/MAGIC populations

advanced Materials

• F2 population (circa 500)of crosses: A-1 x Oker, A-1 x EC-755673-1, A-1 x

EC-736481

• MAGIC lines (circa 350) Methodology

• Selfing of individual plants

Area required

• ICRISAT Farm: 1500 m2

24

Project No. 103-12

Title Exploitation of plant genetic resources for development of

superior inbred lines in castor

Principal Investigator K. Anjani

Co-Investigators M. Santhalakshmi Prasad

P. Duraimurugan

Praduman Yadav

P. Lakashmamma

J. Jawaharlal


Year of start 2015-16

Objectives 1. Development of inbred lines possessing extra-early maturity

(<100 days) & early maturity (120 days), high ricinoleic acid

(around 90% and above), and resistance to Fusarium wilt, and

leafhopper, multiple traits, and pistillate trait from castor

germplasm


Objective 1: Development of inbred lines possessing extra-early maturity (<100 days) &

early maturity (120 days), high ricinoleic acid (around 90% and above),

and resistance to Fusarium wilt, and leafhopper, multiple traits, and

pistillate trait from castor germplasm


K. Anjani Seed multiplication and yield evaluation of

trait-specific inbred lines

Self-seed of promising

trait-specific inbred lines

Seed multiplication and yield evaluation of

capsule borer resistant inbred lines

Promising capsule borer

tolerant inbred lines

Seed multiplication of trait-specific inbred

lines and germplasm accessions

Self-seed of inbred lines

and germplasm

K. Anjani

J. Jawahar Lal

Stability testing of pistillate lines Stable pistillate lines

Praduman Yadav Analysis of inbred lines for oil content Oil data of inbred lines

Activity 1.1: Seed multiplication and yield evaluation of trait-specific inbred lines Material No. of entries: 14 inbred lines + 2 checks, DCS-9, GC3

S. No. Inbred line S. No. Inbred line

1 ICI-EE-GP-63-15 9 ICI-RG-2661-7-3-5-6

2 ICI-EE-GP-73-2 10 ICI-RG-631-8-sp

3 ICI-EE-GP-73-14 11 ICI-RG-3425-5

4 ICI-RG-187-3-2-3-5 12 ICI-RG-2661-7-9-1-7

5 ICI-RG-226-29-2-2 13 ICI-RG-2661-7-5-2

6 ICI-RG-2787-89-20 14 ICI-RG-2722-5

7 ICI-RG-2787-181-12 15 DCS-9 (check)

8 ICI-RG-2746-1 16 GC-3 (check) Design: RBD Replications: 2

Plot size: 10.8 sq.m Spacing: 90 x 60 cm (Rainfed)

Farm: Narkhoda Season: Kharif

25

Observations: 10

1. No. of nodes up to primary spike

2. Plant height (cm)

3. Days to 50% flowering

4. Days to maturity

5. 100-seed weight (g)

6. Seed yield at 90 DAP (g/net plot)



9. Total seed yield (g/net plot)

10. Oil content (%) Activity 1.2: Seed multiplication and yield evaluation of capsule borer resistant inbred

lines

No. of entries: 19 inbred lines + 2 checks (DCS-9, GC3)

S. No. Inbred S. No. Inbred

1 RG-2800-1 12 RG-898-1

2 RG-2800-2 13 RG-898-2

3 RG-2800-3 14 RG-898-3

4 RG-2800-4 15 RG-898-4

5 RG-2800-5 16 RG-898-5

6 RG-2800-6 17 RG-898-6

7 RG-2800-7 18 RG-2048

8 RG-2800-8 19 RG-1628

9 RG-2774-1 20 DCS-9 (check)

10 RG-2774-2 21 GC-3 (check)

11 RG-2774-3

Design: RBD Replications: 2

Plot size/entry: 10.8 sq.m

Spacing: 90 x 60 cm (Rainfed) Farm: Narkhoda Season: Kharif

Observations: 11

1. No. of nodes up to primary spike

2. Plant height (cm)

3. Days to 50% flowering

4. Days to maturity

5. 100-seed weight (g)



8. Seed yield at 180DAP (g/net plot)

9. Seed yield at 210 DAP (g/plot)

10. Total seed yield (g/net plot)

11. Oil content (%)

Activity 1.3: Seed multiplication

A. Trait-specific inbred lines


B. Germplasm accessionsA

No. of accessions: 242

Plot size: 10.8 sq.m Spacing: 90 x 60 cm Farm: Narkhoda

26

Activity 1.4: Stability testing of pistillate lines

No. of lines: 23

Plot size: 10.8 sq.m Spacing: 90 x 60 cm Farm: Narkhoda

Season: Kharif and early summer

Total land requirement: 4500 sq.m

Activity 2: Screening inbred lines against Fusarium wilt


M. Santha Lakshmi Prasad Evaluation of trait specific inbred

lines under sick plot conditions

and glass house conditions

Identification of wilt

resistant inbred lines

under sick plot and

pot conditions Methodology A. Screening under wilt sick plot conditions

Materials: 20 Trait specific inbred lines (Seed to be supplied by Dr. K. Anjani)

ICIRG22-7-2-10-3 ICIRG26-1-3-2-5 ICIRG66-2511-3 ICIRG226-29-2-2

ICIRG22-7-2-10-7 ICIRG187-3-2-3-5 ICIRG66-825-1 ICIRG-392-5

ICIRG15-5 ICIRG187-6-2-1-3 ICIRG66-825-7 ICIRG-2816-1

ICIRG15-3 ICIRG190-2-1-9-14 ICIRG226-29-6-2 ICIRG-2719-9

ICIRG26-1-2-3-14 ICIRG1591-1 ICIRG226-29-7-2 ICIRG-2035-9

Design: ABD + JI-35 and 48-1 after every 5 rows

Plot size: 1-2 rows/entry; Spacing: 60 × 45 cm

Row length: 6.0 m; Date of sowing: 1st fortnight of July, 2019

Season: Kharif; Farm: Rajendranagar (wilt sick plot)

Observations: Germination, wilt incidence at 30 days interval

B. Wilt resistance confirmation under pot culture conditions

Material: 12 trait-specific inbred lines (Seed to be supplied by Dr. K. Anjani)

Inbred lines

ICIRG-2661-7-3-5-6 ICIRG-2746-1

ICIRG-2661-7-9-1-3 ICIRG-2800-2

ICIRG-2787-181-12 ICIRG-2800-4

ICIRG-2787-89-20 ICIRG-2774-1

ICIRG-2787-152-9 ICIRG-2774-2

ICIRG-3425-5 ICIRG-898-6

Method: Sick pot method

Replications: 4 (6 plants in each): Date of sowing: 1st fortnight December, 2019

Observations: Wilt incidence up to 30 days after sowing

Activity 3: Screening of inbred lines against insect pests


P. Duraimurugan Confirmation of resistance to capsule

borer in inbred lines

Screening of inbred lines against

whitefly

Whitefly and capsule

borer resistant inbred

lines

27

A. Confirmation of capsule borer tolerance in inbred lines Materials: 17 inbred lines from RG-2800, RG-2774, RG-898

(Seed to be supplied by Dr. K. Anjani).

Resistant check: 48-1 & Susceptible check: DCS-9) Design: RBD Replications: 2 Spacing: 90 x 60 cm Date of sowing: II fortnight of August Plot size: One row of 6m/ genotype Method: Infester row technique with DCS-9 (one infester row after every 2 entries in sandwich method) Land requirement: 500 m2 Farm: Rajendranagar Observations:

• Capsule borer damage will be recorded both in the primary and secondary spikes. • Percentage capsules damaged will be presented by recording the total number of capsules and number of damaged capsules per plant

B. Screening of inbred lines against whitefly

Material: 13 (8 inbred lines from RG-2800, Resistant checks: DPC9, DCH-177;

susceptible checks: M-574, VP1, DCH-519)-Seed of inbred lines to be

supplied by Dr. K. Anjani

Design: RBD Method of screening: Infester Row Technique Date of sowing: December II FN Plot size: 6m row Replications: 2 Spacing: 90 x 60 cm Land requirement: 600 m2 Farm: Narkhoda

Observations:

• Whitefly population (nymphs and pupae on 0 to 5 scale) • Whitefly adult population from fully developed top leaf (feeding preference)

Activity 4. Identification of drought tolerant germplasm accessions


P. Lakshmamma Screening of germplasm for drought

tolerance and temperature tolerance

Drought and temperature

tolerant accessions

A. Screening castor germplasm accessions for drought tolerance (stress from 30-90

DAS) in field

Genotypes: 12 accessions (RG-2818, RG-2822, RG-2296, RG-1663, RG-3798, RG-1594, RG-2067, RG-1587, RG-2149, RG-2781, RG-2787, RG-2149 along with checks (48-1, DCH-519) - (Seed to be supplied by Dr. K. Anjani)

Treatments: 2 (Control, Drought stress (30-90 DAS) Replications: 3 Spacing: 90 x 60 cm; Plot size: 4.5 x 6.0 m (5 rows) Design: Split plot Season: late rabi, Nov, 2019 Observations: Growth, TDM before relieving stress, Yield, yield components Farm: Narkhoda Land required: 3000 m2

B. Screening identified drought tolerant castor germplasm lines for temperature

tolerance

Genotypes: 15 (RG-27, RG-72, RG-82, RG-89, RG-111, RG-298, RG-1437,

RG-1494, RG-1826, RG-1941, RG-2048, RG-2139, RG-2797, 48-1,

DCH-519) (Seed to be supplied by Dr. K. Anjani) Sowings: 2 (Nov. & Jan. 1st week) Replications: 3 Spacing: 90 x 60 cm; Plot size: 2.7 x 6.0 m (3 rows) Design: RBD Observations: Days to flowering and maturity, TDM at harvest, yield Farm: Narkhoda Land required: 2000 m2

28

Project No. 103-13

Title Diversification of parental base and development of superior

parental lines in castor

Principal Investigator T. Manjunatha

Co-Investigators C. Lavanya

S. Senthilvel

P. Lakshmamma


Year of start 2015

Objectives 1. To develop wilt, gray mold and leafhopper resistant

monoecious male lines with good combining ability, high

oil (48-50%) and ricinoleic acid (87-90%)

2. To identify diverse sources and develop wilt and

leafhopper resistant pistillate lines with good combining

ability using recombination breeding techniques

3. To develop wilt and leaf hopper resistant, early (90-100

DAS), medium /late (100-120 DAS) duration, high

yielding hybrids (>2.5t/ha rainfed; >3.5 t/ha irrigated)

suitable for early, late kharif and rabi seasons in traditional

and non-traditional castor growing states of the country (as

Linkage with AICRP castor)

Experiments/Activities (2019-20) Objective 1: To develop wilt, gray mold and leafhopper resistant monoecious male lines

with good combining ability, high oil and ricinoleic acid


S. Senthilvel

C. Lavanya

T. Manjunatha

P. Lakshmamma

Advancement of single plant selections

from segregating generations

Promising individual

plants for stabilization

Materials

S1 progenies of 215 plants selected from genepool for gray mold resistance

151 F3, 27 F4, 75 F5, 67 F6 families of 31 bi-parental crosses, BC1F2 of DPC-9 x RG-

27

(71 F3 families, 15 F4 families, 60 F5 and 36 F6 families, BC1F2 of DPC-9 x RG-27) Methodology

Method: Selection & selfing

Spacing: 90 × 60 cm

Season: Kharif

Date of sowing: 2nd fortnight of June 2019

Land required: 6000 m2 for Kharif

Farm: Narkhoda


T. Manjunatha

Multiplication and maintenance of farmer’s

collections and generating 20 new crosses for

male line development using farmers’s collections,

wilt resistant monoecious lines and drought

tolerant accessions (RG-2048, RG-1628, RG-

2124, RG-2127)

Diversification and

development of

monoecious lines

29

Materials: 224 farmer’s collections Methodology

Method: Hybridization between farmer’s collections and wilt resistant elite

monoecious lines and drought tolerant germplasm lines based on the desired traits

Spacing: 90 × 60 cm,

Season: Kharif


Land required: 0.2 ha

Farm: Narkhoda Objective 2: To identify diverse sources and develop wilt and leafhopper resistant

pistillate lines with good combining ability using recombination breeding

techniques


C. Lavanya Development of genepool for pistillate

trait

Single plant selections for

pistillate trait up to late orders Material

F2 of Rb-1854 with pollen mixed from DPC-9, SKP-84 and JP-86 Methodology

Method: Single plant selection and selfing


Season: late kharif and extended to Rabi (with assured irrigation facilities)

Date of sowing: 1st fortnight of September 2019


Farm : Narkhoda


C. Lavanya

T. Manjunatha

Selection and generation advancement of

agronomically superior single plant

selections / progenies for pistillate trait

A set of individual

pistillate plants selected

for further stabilization Materials:

Four quadruple crosses derived from two double crosses (DPC 25,R3SP x Rb 13-

1854,R2SP) X (CNES-1,R0NSP) x NES-6,R2NSP) and (DPC 21,G3SP x DCS-106,

G3NSP) X (JP-77-1,G1SP x DPC-21,G2SP),

11 F2 progenies from two double crosses, 3 F2 progenies from 2 single crosses

21 + 3 BC1F2 progenies from six crosses viz., [(CNES-1 x FC-8) x CNES-1],

[(CNES-1 x PMC-36) x CNES-1], [(M-619 x FC-8) x M-619], Kh-13-154 x M-574,

Kh-13-154 x DPC-19, Kh-13-154 x DPC-9

71 F3 selections derived from six bi-parental crosses (Rb 13-1854, x DPC 25, CNES-

1 x NES-6, JP-77-1 x DPC-21, DPC-21 x DCS-106, DPC-25 x Rb 13-1854 and

M619 x 48-1) for pistillate line diversification.

Double crosses: between (DPC-16 x M-571) x (SL x DPC-25) and (DPC-23 x DPC-

21) x (DPC-9 x DPC-14)

F2 of DPC-23 x DPC-21 Methodology

Method: Pedigree breeding

No. of rows: F2-20, BC1F2-4,P1-2,P2-2 rows each


30

Season: Kharif and Rabi

Date of sowing: 2nd fortnight of June, 2019


Farm: Narkhoda

Observations: Morphological characters of individual plants, pistillate expression Objective 3: To develop wilt and leaf hopper resistant, early, medium/late duration

hybrids suitable for early, late kharif and rabi seasons in traditional and

non-traditional castor growing states of the country


T. Manjunatha

C. Lavanya

Studying the combining ability of new

inbred lines

Promising parental lines for

hybrid development Materials: 114 hybrids in 6 sets along with 2 checks-GCH-8, ICH-66, 39 Females and

20 males

Set Lines Testers No. of hybrids

Set-I DPC-22, DPC-25, IPC-30,

IPC-31 and JP-86

ICS-310, ICS-312 and ICS-313 15*

Set-II DPC-22, DPC-25, IPC-42,

IPC-44

ICS-125, ICS-127 12*

Set-III IPC-40, IPC-41, IPC-42,

IPC-45, VP-1

ICS-164 5*

Set-IV IPC-38, IPC-39 DCS-78, DCS-89, DCS-94,

DCS-102, DCS-104,DCS-

105,DCS-107, DCS-108,DCS-

110,DCS-112, DCS-118, DCS-

119

32

Set-V DPC-16, DPC-18,DPC-23,

DPC-27, DPC-28, M-571,

M-574,NES-6, JP-86, IPC-

33, IPC-34,IPC-38, IPC-39

DCS-89, DCS-119 26

Set-VI 24 females DCS-119* 24* * Lab studies for Ricinoleic acid content in hybrids and their parents – Dr. Praduman Yadav

Methodology


Plot size: 1 row of 6 m length

Design: RBD, 3 replications

Season: Kharif

Observations: Days to 50 % flowering

Number of nodes to primary spike

Plant height (cm)

Total spike length (cm) of primary

Number of capsules / primary spike

Days to maturity of primary spike

Number of effective spikes per plant

100 seed weight (g)

Pick wise seed yield (g/plant)

Oil content (%)



Farm: Narkhoda

31


T. Manjunatha

C. Lavanya

S. Senthilvel

Preliminary evaluation of experimental

hybrids in kharif

Promising cross

combinations for

hybrid development

Materials: 189 experimental hybrids and 2 checks (GCH-8 and ICH-66) in four sets

Sets Female Male No. of

Hybrids

1 M-571, M-619, DPC-21,

DPC-23, DPC-25, IPC-38,

IPC-42, IPC-44, IPC-46

ICS-324, ICS-325, ICS-341, ICS-346,

ICS-348, ICS-349, ICS-350, ICS-351,

ICS-353, ICS-354, ICS-355, ICS-356

54

2 DPC-9, DPC-15, DPC-16 DCS-78, DCS-89, DCS-94, DCS-102,

DCS-104, DCS-105, DCS-107, DCS-108,

DCS-109,DCS-110, DCS-112, DCS-118,

DCS-119, DCS-120, DCS-121,48-1

60

3 DPC-22, DPC-25, IPC-30,

IPC-31, IPC-40, IPC-41,

IPC-42, IPC-43, IPC-44,

IPC-45, IPC-46, JP-86,

DPC-15

DCS-94, ICS-109, ICS-125, ICS-127,

ICS-133, ICS-137, ICS-142, ICS-144,

ICS-164, ICS-174, ICS-304, ICS-305,

ICS-310, ICS-312, ICS-313, ICS-314,

ICS-315, ICS-316, ICS-318, ICS-321,

ICS-322, JI-315, FC-08

49

4 M-574, DPC-21, SKP-84 ICS-121, ICS-127, ICS-128, ICS-130,

ICS-132, ICS-33, ICS-134, ICS-139, ICS-

140, ICS-141

26

Methodology


Plot size: 1 row of 6 m length

Design: RBD, 3 Replications

Season: Kharif



Plant height (cm)


Number of capsules / primary spike



100 seed weight (g)

Pick wise seed yield (g/plant)

Oil content (%)



Farm: Narkhoda


S. Senthilvel

C. Lavanya

Confirmatory yield trial of selected

hybrids in kharif

Promising cross combinations

for AICRP trial

Materials: 15 hybrids with 2 checks (DCH-177 and GCH-8)

S. No. Hybrid Parentage

1 ICH-880 DPC-14 x ICS-326

2 ICH-901 DPC-25 x ICS-326

32

3 ICH-904 DPC-25 x ICS-339

4 ICH-909 DPC-25 x ICS-325

5 ICH-884 DPC-18 x DCS-94

6 ICH-887 DPC-18 x DCS-119

7 ICH-888 DPC-18 x DCS-121

8 ICH-941 DPC-27 x DCS-89

9 ICH-747 DPC-18 x GP-674

10 ICH-755 DPC-25 x GC-3

11 ICH-762 DPC-18 x GP-538

12 ICH-816 DPC-25 x GP-764

13 ICH-347 DPC-21 x JI-340

14 ICH-368 M-571 x DCS-89

15 ICH-365 M-571 x DCS-78

Methodology


Plot size: 4 rows of 6 m length

Design: RBD with 3 replications

Season: Kharif



Plant height (cm)


Productive spike length (cm) of primary



100 seed weight (g)

Pick wise seed yield (g/plot)

Oil content (%)

Date of sowing: 2nd fortnight of June

Land requirement: 1300 m2

Farm: Narkhoda


T. Manjunatha

S. Senthilvel

Generation of new experimental

hybrids

100 new cross combinations

for further testing

Materials

Female lines Male lines

M-571, M-619, DPC-21, DPC-22,

DPC-25, IPC-30, IPC-38, DPC-25,

IPC-42, IPC-43, IPC-44, IPC-45,

IPC-30, IPC-31, VP-1,

ICS-125, ICS-127, ICS-164, CS-310, ICS-312, ICS-

313, ICS-324, ICS-325, ICS-326, ICS-327, ICS-330,

ICS-335, ICS-336, ICS-339, ICS-349, ICS-356

Methodology


Plot size: 2 rows of 6 m length for male & 6 rows of 6 m length for female

Season: Rabi

Date of sowing: 1st fortnight of October

Land required: 3000 m2

Farm: Narkhoda

33


S. Senthilvel

T. Manjunatha

C. Lavanya

Seed production of hybrids for

IHT/AHT

Seeds of 6 hybrids

available for IHT/AHT

Materials:

IVHT-2020-21 AVHT-2020-21

ICH-243 ICH-278

ICH-239 ICH-515

ICH-277

ICH-382


Season: Rabi

Date of sowing: 1st fortnight of September

Land required: 6 × 400 m2

Farm: Narkhoda or ICRISAT


S. Senthilvel

T. Manjunatha

C. Lavanya

Maintenance breeding of parental lines Parental lines of released

and pre-release hybrids

Material:

Released: M-574, SKP-84, DPC-9, DCS-9, DCS-78, ICS-164

Pre-release: 30 pistillate and 70 male lines


Season: Kharif and Rabi

Date of sowing: June /September (with assured irrigation facilities)

Land required: 3000 m2

Farm: Narkhoda


P. Lakshmamma

C. Lavanya

Evaluation of hybrids along with

parents for root growth (Poly bags)

Promising drought resistant

cross combinations

Materials

Treatments/materials: 14 (DCH-177, DPC-9, DCS-9, DCH-519, M-574, DCS-78, ICH-440,

DPC-15, ICS-345, ICH-66, SKP-84, ICS-164, ICH-278, DPC-25,

ICS-164)

Methodology: Poly bags

Design: CRBD, Replications:4, 3 plants per replication

Farm: Narkhoda

Season: late kharif (August)


Plot size: 4 rows of 6 m length

Season: late kharif (August)

Observations: Root length, volume, biomass and plant dry matter at

90 DAS

34


P. Lakshmamma

Evaluation of parental lines for drought

tolerance in field

Promising drought

resistant parental lines

No. of Genotypes: 15 (DCS-9, DCS-104, DCS-105, DCS-106, DCS-110, DCS-119,

DPC-9, DPC-16, DPC-18, DPC-21, DPC-23, DPC-28, ICS-121,

ICS-127, ICS-133, ICS-134, ICS-322) along with 2 checks

Treatments: Control, drought stress from 30-90 DAS

No. of rows: 3

Replications: 3

Design: Split plot

Spacing 90 x 60cm, Plot size: 2.7 x 6.0 m (3 rows)

Farm: Narkhoda

Season: late rabi (November)

Area: 3000 m2

Observations: Growth, TDM before relieving stress, at final harvest, Yield,

yield components


P. Lakshmamma

C. Lavanya

Selection and generation advancement of

BC1F2 of DPC-9 x RG-72 for drought

tolerance (by imposing drought stress from

30-90 DAS) and selfing single plants after

relieving stress

Promising drought

resistant breeding

material

Methodology: Field screening under stress

No. of plants: 500

Spacing: 90 x 60cm

Farm: Narkhoda

Season: late rabi (October/November)

35


Objective 1: To develop ‘marker tool kit’ for wilt resistance breeding


S. Senthilvel

M. Santha Lakshmi

Prasad

Assess marker-trait co-segregation

for wilt resistance in segregating

populations

Confirmation of linkage

between marker and wilt

resistance in five resistant

sources

Materials

F2 populations of 48-1 × JI-35, RG-1149 × JI-35, RG-2685 × JI-35, RG-1354 × JI-35

and RG-1673 × JI-35 (~150 individuals per cross combination)

Methodology

Scoring disease reaction of F2 individual sown in wilt sick plot at 150 DAS

Genotyping F2 individuals at 10-20 SNP loci linked to wilt resistance through KASP

assay and analyzing the linkage between marker genotype and wilt resistance

Objective 2: To establish gene diversity for wilt resistance and develop gene specific

near isogenic lines


M. Santha Lakshmi Prasad

S. Senthilvel

Perform allelism test Allelic relationship of wilt resis-

tance genes in RG-1149 and 48-1

Materials

~200 F2 plants of RG-1149 × 48-1

Project No. 103-14

Title Development of genomic resources and tools for applications

in castor breeding

Principal Investigator Senthilvel Senapathy (Plant Breeding)

Co-Investigators R. D. Prasad (Plant Pathology)

M. Santha Lakshmi Prasad (Plant Pathology)

B. Gayatri (Nematology)


Year of start 2017

Objectives 1. To develop ‘marker tool kit’ for wilt resistance breeding in

castor

2. To establish gene diversity for wilt resistance in castor and

develop gene specific near isogenic lines

3. To validate/detect novel QTLs associated with resistance to

gray mold in castor

4. To detect QTLs associated with resistance to reniform

nematode in castor

36

Methodology

Scoring F2 population for wilt resistance at sick plot

Assessing the segregation of wilt resistance through chi-square test


S. Senthilvel Generate backcross populations

towards development of NILs

BC3F1 , BC2F2& BC1F2 families

for further advancement

Materials

[(JI-35 × RG-999) × JI-35] BC2F1, [(JI-35 × RG-1673) × JI-35] BC1F2& [(JI-35 ×

48-1) × JI-35] BC1F1

Methodology

Raise the BC2F1 plants of JI-35 × RG-999 in sick plot and cross the selected plant

with JI-35

Raise the BC1F2 plants of JI-35 × RG-1673 in sick plot and cross the selected plant

with JI-35; Raise the BC2F1 plants in the field and self the selected plants

Raise the BC1F1 plants of JI-35 × 48 in the field and self the selected plants

Objective 3: To validate/detect novel QTLs associated with resistance to gray mold


R.D. Prasad

S. Senthilvel

Validate the putative QTL linked to

gray mold resistance in 48-1

Genomic regions associated with

gray mold resistance validated

Materials

75 F2 plants of DPC-15 × 48-1

Methodology

Scoring ‘disease severity’ of individual plants using ‘detached spike/ capsule

technique’

Genotyping of F2 population using SNP markers flanking the QTL region(s)

37


Plant material to be used: DCS-107, 48-1 (Jwala), M-574, DCS-78 Objective-1: To develop transformation protocols in castor through tissue culture and

in planta methods Scientist(s) Activities

M. Sujatha, B. Usha Kiran HHK Swamy

Optimization of in vitro regeneration approaches

V. Dinesh Kumar B. Usha Kiran

In planta transformation approach using seedlings

Place of experimentation: All the experiments will be carried out in the tissue culture

facility available at IIOR. Activity-wise technical programme Activity 1: Optimization of in vitro regeneration approaches

Scientist(s) Type of experiment Deliverable(s)

B. Usha Kiran, HHK Swamy, V. Dinesh Kumar M. Sujatha

Lab Optimized in vitro regeneration pro-tocols, either through organogenesis or somatic embryogenesis

Experiment 1: Evaluation of 10 promising combinations for organogenesis with

Different genotypes (HHKS) Plant material: 46 genotypes: DCS-407, 48-1, ICS-427, ICS-428, ICS-430, ICS-432, ICS-433, ICS-434, ICS-

435, ICS-436, ICS-437, ICS-439, ICS-440, ICS-444, ICS-442, ICS-443, ICS-444, ICS-445,

ICS-446, ICS-447, ICS-448, ICS-449, ICS-450, MCI-3, MCI-8, JI226, JI227, JI345, JI349,

JI338, JI340, GP493, GP778, GP788, DCS9, DCS78, DCS402, DCS404, DCS405, DCS407,

DCS408, DCS409, DCS440, DCS442, DCS448, DCS424 (provided by Dr. Lavanya) Explant: Petiole, Cotyledonary node, Hypocotyl explants Media: 9 media (RM 4, 5, 6, 7, 11, 13, 23, 28, 30) combinations identified previously for

initiation of organogenesis will be tested with the explants. Methodology:

Effect of different genotypes, explants and chosen regeneration media will be

determined

Experiments will be laid out in FCRD having 3 replications, 30 explants/rep

Project No. 103-15

Title Optimization of regeneration and transformation protocols to realize grey mold resistant transgenic castor (Ricinus communis L.)

Principal Investigator V. Dinesh Kumar Co-Investigators M. Sujatha

B. Usha Kiran HH Kumara Swamy

Co-operating Centre PI Rohini Sreevathsa, NRCPB, IARI, New Delhi PIMS Number IXX13580 Year of start 2017 (June) Objectives 1. To develop transformation protocols in castor through tissue

culture and in planta methods 2. To realize transgenic castor plants using single gene and

multi-gene constructs developed at IIOR using the standardized protocol(s)

3. Characterization of the obtained transgenic plants

38

All the experiments will be carried out in the tissue culture facility Observations:

Data to be recorded - responsive explants (%), days required for shoot initiation,

number of shoots per explant, length of visible shoots, number of visible leaves/shoot

and regeneration frequency (%) Experiment 2: Evaluation of explants types on different (new) media for morphogenic

callus induction and regeneration of shoots (UK) Plant material: DPC-9 Explant: Petiole, Cotyledonary node, Hypocotyl Hormonal combinations: 150 Methodology:

Determining the suitable concentrations and combinations of plant growth regulators

(in different media) for efficient shoot formation from shoot initiation, elongation and

rooting.

Experiments will be laid out in FCRD having 3 replications, 30 explants/rep

All the experiments will be carried out in the tissue culture facility Observations:

The type and amount of callus formed, number of shoots regenerated from callus, no

of shoots elongated, % of root formation on a specific medium after different days of

its inoculation, % of plants acclimatized. Experiment 3: Evaluation of different explants on different media for somatic

Embryo genesis (UK and VDK) Plant material: DPC-9 Explant: Hypocotyl, cotyledons Hormonal combinations: 2, 4-D, 2, 4, 5-T, Picloram, Dicamba, BAP, TDZ Number of combinations: More than120 Methodology

Assessment of different explants viz., cotyledon, hypocotyl, meristem etc on various

media combinations for induction of somatic embryos. Transferring of the responding

explants/embryos at one stage of development to different media to favour further

development or differentiation and obtain complete plantlets.

If embryos are obtained, microtomy studies will be taken up to understand ontogeny

of somatic embryos. Experiments will be laid out in FCRD having 3 replications, 30

explants/rep and all the experiments will be carried out in the tissue culture facility Observations

Data to be recorded - The number of explants showing embryogenic calli, explants

with different stages of development of the embryos, studying the ontogeny of the

embryos, immature somatic embryos obtained from each of the media combinations,

conversion of embryos into plantlets through germination process, % of plantlets with

proper roots on different media and % of acclimatized plants. Activity 2: In planta transformation approach using seedlings (VDK and UK) Experiment 1: Evaluation of the available T2 seeds from the PCR positive T1 plants

(VDK and UK) Methodology

Available T2 seeds from the remaining 8 PCR positive T1 plants (for EBP1) will be

sown in the transgenic green house and maintained till maturity. These plants will be

evaluated for the presence of the transgene(s) they carry using gene specific and

construct specific markers.

39

Observation Presence or absence of the transgene(s)/construct specific amplicons from the plants as seen through agarose gel electrophoresis after genomic PCR.

Experiment 2: Evaluation of the available T3 seeds from PCR positive T2 plants

(VDK & UK) Methodology

Available T3 seeds from 8 PCR positive T2 plants (for EBP1) will be sown in the transgenic green house and maintained till maturity. These plants will be evaluated for the presence of the transgene(s) they carry using gene specific and construct specific markers.

Observation

Presence or absence of the transgene(s)/construct specific amplicons from the plants

as seen through agarose gel electrophoresis after genomic PCR. Experiment 3. Attempting in planta transformation with the multigene construct

(VDK, UK and MS) Objective 2. To realize transgenic castor plants using single gene and multi-gene

constructs developed at IIOR using the standardized protocol(s)

Scientist(s) Activities M. Sujatha, B. Usha Kiran HHK Swamy

To develop transgenic castor using optimized in vitro transformation protocol with the gene constructs

V. Dinesh Kumar, B. Usha Kiran M. Sujatha

Developing the transgenic castor using the optimized in planta transformation approach

Activity-wise technical programme Activity 1: Developing transgenic castor using optimized in vitro transformation protocol –

with the gene constructs (MS. UK and HHKS) Experiment 1: Assessing the amenability of in vitro regeneration protocol for transformation Methodology

Optimization of parameters such as, stage at which Agro treatment to be given, the age and cell density of the Agro culture and factors such as induced vir genes, effect of expression of antibiotic genes on regeneration, etc that are known to affect the transformation efficiency using EHA105 as well as LBA4404 strain harbouring gene construct with gus and/or GFP under 35 S promoter.

Observation

Assess the gus/GFP expression in tissues at different time points after the

transformation procedure Experiment 2: Transformation with the optimized protocol (MS. UK and HHKS) Methodology

Optimized in vitro protocol to be used for transforming seedling or embryo derived

explants using the single or multigene constructs Observation

Assess the presence of transgene(s) in the putative shoots/plants Activity 2: Developing the transgenic castor using the optimized in planta transformation

approach Experiment 1: Transformation of seedlings with gene constructs Methodology

Based on the optimized transformation method, at least 300 seedlings of castor will

be subjected to in planta transformation; Two or three constructs will be tried for this

purpose Observation

Survival and T1 seed production from the plants.

40

Project No. 104-12

Title Agronomic interventions for increasing productivity and

resource use efficiency (nutrient and moisture) of emerging

cropping systems involving oilseeds

Principal Investigator S.N. Sudhakara Babu

Co-Investigators Md. Aziz A. Qureshi


Year of start 2015

Objectives 1. To assess dynamics of productivity, profitability, nutrient

use efficiency and sustainability of long term fertility

management in Maize – castor cropping system in Alfisols

(Modified cropping system as decided in the IRC from 2018-

19)

2. To assess productivity, profitability and resource use pattern

of new and emerging crop sequences involving sunflower in

Alfisols - Completed

3. Statistical assessment of long term fertilizer experiment -

Completed


Objective 1: To assess dynamics of productivity, profitability, nutrient use efficiency

and sustainability of long term fertility management in Maize – Castor

cropping system in Alfisols

Experiment 1. Long term fertilizer studies on Maize – Castor cropping system in Alfisols.

Year of start: 1998-99


S.N. Sudhakara Babu Maintenance of fixed plot long term

nutrient management trial in Alfisols.

Planning and execution

of field experiments,

growth and yield data

collection, processing,

analysis, coordination

and reporting.

Growing kharif Maize in the

designated 14 nutrient management

treatments.

Field crop management,

crop observa-tions,

sampling, coordination,

data compilation,

analysis and reporting.

Growing rabi Castor in the designated

14 nutrient management treatments.

Field crop management,

crop observa-tions,

sampling, coordination,

data compilation,

analysis and reporting.

Md. Aziz A. Qureshi Soil and plant analysis of sorghum and

sunflower crops.

Soil and plant analysis

nutrient uptake and soil

nutrient balance studies.

Methodology: Field experiment on fixed plot with 14 treatments and three replications

41

Treatments/materials:

Treatment No. Maize (kharif) Castor (rabi)

1 N N

2 NP NP

3 NPK NPK

4 50% NPK 50% NPK

5 150% NPK 150% NPK

6 NPK + Crop Residue Incorporation NPK

7 NPK+FYM @ 5t/ha NPK

8 NPK+ S@ 20kg/ha through Elemental S NPK

9 NPK+ B@ 1kg/ha as borax in alternate years NPK

10 NPK+ Zn@ 5kg/ha as zinc oxide NPK

11 NPK+ S + B + Zn NPK

12 No Manure/Fertilizer No

manure/fertilizer

13* FYM @ 5t/ha (30kg N/ha) FYM @ 5t/ha

(30kg N/ha)

14* FYM + Goat Manure + Poultry manure

(1/3 each N equivalent)

FYM + Goat

Manure + Poultry

manure (1/3 each N

equivalent)

*= From 2016 onwards

NPK (100% RDF) = Maize: STCR based; Castor: STCR based for 100% RDF kg N;

P2O5:K2O/ha

Design: RBD Replications: 3 Plot size: 7.2m x 5.0m

Maize (Kharif) Castor (Rabi)

Variety : Syngenta 6240 hybrid DCH-519

Spacing: 60cm x 30cm 120cm x 90cm

Date of sowing: June 2019 October 2019

Observations:

Initial plant population after thinning

Final plant population at harvest

Dry matter production and distribution at 30DAS, 50% flowering and at harvest

Days for 50% flowering and maturity

1000 seed weight, fresh and dry stover weight

Seed yield (g/plot) and Kg/ha

Oil content for castor

Weather data

Soil and Plant analysis of maize and castor

Farm: Narkhoda Research Farm of IIOR: G3 Fixed Plot – 2000 m2

Date of sowing: June 2019

42

Objectives

1. To quantify the productivity and RUE of safflower based cropping systems under

different land configurations

2. To assess nutrient management and crop geometry for safflower in system mode

under different land configurations under variable soil moisture availability

Experiments/Activities (objective-wise) Objective 1: To quantify the productivity and RUE of safflower based cropping systems

under different land configurations


P. Padmavathi Assess the productivity of safflower based cropping systems

Efficient safflower based cropping system/s in terms of system productivity, moisture use and nutrient use under different land configurations

Assess moisture use of safflower based cropping systems

Aziz Qureshi Assess nutrient use of safflower based cropping systems

Objective 2: To assess IPNM and crop geometry for safflower in cropping system mode

in different land configurations under variable soil moisture availability


P. Padmavathi Assess impact of cropping system mode safflower crop geometry and IPNM on system productivity

Optimum combination of crop geometry and IPNM for safflower in different cropping systems under different land configurations

Aziz Qureshi Assess impact of cropping system mode safflower crop geometry and IPNM on system nutrient use

Assess impact of cropping system mode safflower crop geometry and IPNM on soil nutrient availability

P. Ratna Kumar Assess the impact of kharif crop, safflower plant geometry and IPNM on safflower physiology

Safflower physiological response to crop geometry and IPNM

K. Alivelu Assess statistical significance/non-significance effect of different treatments on crop productivity/ system productivity

Cropping systems productivity variation across three locations Best combination of crop geometry and IPNM under different land configurations

Materials Methodology IIOR (BBF 1.2 + 0.3 m) Main plot (kharif): 1. Greengram

2. Soybean (ND)

3. Soybean (SD)

Project No. 104-13

Title Assessing safflower based cropping systems productivity and resource use efficiency under different land configurations, crop geometry and IPNM in different rainfall patterns

Principal Investigator P. Padmavathi

Co-investigators Aziz Qureshi P. Ratna Kumar K. Alivelu


Year of start 2015

43

a) 4 rows/BBF with RDF (P as SSP) + deficient micronutrient + Rhizobium

b) Leaving crop residues in the field Subplot (safflower): Factorial combination:

a) Plant geometry: i) 3 rows/BBF

ii) 2 rows/BBF b) IPNM: i) No fertilizer

ii) 50% RDF + + Azot/Azos + PSB

iii) RDF + Azot/Azos + PSB Design: Split factorial Replications: three Plot size (subplot): 5 BBF x 6 m

Location: ICRISAT watershed Land area: 4000 m2 Parbhani (BBF 1.5 + 0.6 m) Main plot (kharif): 1. Soybean (SD)

2. Soybean (ND)

a) 5 rows/BBF with RDF (P as SSP) + deficient micronutrient + Rhizobium

b) Residues back to field Subplot (safflower): Factorial combination:

a) Plant geometry: i) 4 rows/BBF

ii) 3 rows/BBF b) IPNM: i) No fertilizer

ii) 50% RDF + Azot/Azos + PSB

iii) RDF + Azot/Azos + PSB Design: Split factorial Replications: three Plot size (subplot): 4 BBF x 6 m Annigeri (Compartmental bunding 10 x 10 m) Main plot (Kharif): 1. Fallow

2. Greengram a) Flatbed sowing (30 x 10 cm plant spacing) with RDF + Rhizobium

b) Residues back to field Subplot (Safflower): Factorial combination:

a) Plant geometry: i) 30 x 20 cm

ii) 45 x 20 cm iii) 60-30-60 cm (paired row) b) IPNM: i) No fertilizer

ii) 50% RDF + Azot/Azos + PSB

iii) RDF (P as SSP) + deficient

micronutrient + Azot/Azos + PSB Design: Split factorial Replications: two Plot size (subplot): 10 x 10 m2 Observations: Soil moisture content at monthly interval

Profile soil moisture at sowing of both kharif and rabi crops

RWC, chlorophyll content, canopy temperature in safflower (at flowering and seed

filling stages)

Soil nutrient availability at sowing (kharif crop) and at harvest (rabi crop)

Crop nutrient uptake (N, P and K) at harvest by both kharif and rabi crops

Seed and biological yield

Protein (legumes) and oil content (safflower)

44

Project No. 104-14

Title Synthesis and evaluation of polymers for seed health and productivity of oilseed crops

Principal Investigator K.S.V.P. Chandrika Co-Investigators Praduman Yadav

R.D. Prasad S.N. Sudhakara Babu P.Padmavathi Lakshmi Prayaga P. Ratna Kumar

Co-operating Centre PI

Anupama Singh (Agril. Chemicals), ICAR-IARI, New Delhi Tushar Jana (Chemistry), UoH, Hyderabad

PIMS Number IXX13048 Year of start 2016 Objectives 1. Synthesis and characterization of biopolymer materials for

seed coating 2. Optimization of biopolymers using surfactants 3. In vitro and in vivo evaluation of treated seeds in comparison

with non-treated seeds 4. Water absorbency of Pusa Hydrogel in red and black soils at

different temperatures by gravimetric analysis (in vitro) 5. Assessment of Pusa Hydrogel impact on (a) irrigation water

reduction and productivity of sunflower and (b) productivity of safflower


Experiment 1: Effect of polymeric films on soil microfauna and its degradation over a

period of time


K.S.V.P. Chandrika Soil respiration Carbon dioxide release of microbes can be determined

R.D. Prasad Microbial activities through plating method

Soil bacteria, fungi and actinomycetes intensity will be studied

Praduman Yadav Soil biochemical activity - Methodology:

Screw cap method

Titration method

Biochemical analysis using colorimetric and spectroscopic method Treatments:

Control

Polymeric films

Polymer + Trichoderma films Observations:

Carbondioxide

Microbial count (bacteria, fungi and actinomycetes) over period of time

Soil biochemical and enzymes quantification Experiment 2: Evaluation of developed seed coating polymers in soybean.


K.S.V.P. Chandrika Seed coating in soybean along with bio agents (Trichoderma & Rhizobium)

Uniform seed coating along with bio agents

K. Parimala, Scientist (Plant breeding), SRTC, Hyderabad

To assess the effect of polymer seed treatment as an adjuvant on seed yield and its parameters (Test crop: Soybean)

Performance of polymers at field level

45

Materials & Methods

Season : Kharif, 2019

Crop and Variety : Soybean, JS-335

Treatment details : Design : RBD Replications : 3 T0 - Control T1 - Rhizobium T2 - Polymer 1 T3 - Polymer 2 T4- Trichoderma T5 - Polymer 1+Th T6 - Polymer 2+Th T7 - Rhizobium + Polymer 1 T8 - Rhizobium + Polymer 2

Observations: : Field evaluation: Yield attributes

Experiment 3: To assess the effect of polymer seed treatment on seed yield and its

parameters in groundnut


K.S.V.P. Chandrika Seed coating in groundnut along with

bio agents (Trichoderma & fungicide)

Uniform seed coating

along with bio agents and

fungicide

K. Parimala, Scientist

(Plant breeding),

SRTC, Hyderabad

To assess the effect of polymer seed

treatment on seed yield and its

parameters (Test crop: Groundnut)

Performance of polymers

at field level

Materials & Methods

Season : Kharif, 2019

Crop and Variety : Groundnut, Kadiri-6

Treatment details : Design : RBD Replications : 3

T0 - Control

T1 - Trichoderma

T2 - Polymer 1 + Trichoderma

T3 – Cts + Trichoderma

T4 - Vitavax

T5 – Polymer 1 + Vitavax

T6 – Cts + Vitavax

T7 – Polymer 2 + vitavax

T8 – as per conventional sprays

Observations: : Field evaluation:

Plant stand

Yield attributes

Disease incidence

Experiment 4: To assess the effect of polymer seed treatment on seed yield and its

parameters in sunflower


K.S.V.P. Chandrika Seed coating in groundnut along with

bio agents (Trichoderma & fungicide)

Uniform seed coating along

with bio agents and

fungicide

Dr. SNS Babu, To assess the effect of polymer seed

treatment on seed yield and its

parameters (Test crop: Sunflower)

Performance of polymers at

field level

46

Materials & Methods

Season : Kharif, 2019 Sowing: July, 2019

Crop and Variety : Sunflower, DRSH-1;Spacing: 60 x 30 cm;

Plot size: 5.4 x 4.8 m

Treatment details : Design : RBD Replications : 3

T0 - Control

T1 - Trichoderma

T2 - Polymer 1 + Trichoderma

T3 – Cts + Trichoderma

T4 - vitavax


T6 – Cts + vitavax


T8 – as per conventional sprays

Observations: : Field evaluation:

Plant stand

Yield attributes

Disease incidence

Objective 5: Assessment of Pusa Hydrogel impact on irrigation water reduction and

productivity of sunflower


S.N. Sudhakara Babu Impact of Pusa Hydrogel on irrigation water

use of sunflower

Water use efficiency

and productivity of

sunflower

Lakshmi Prayaga Impact of Pusa Hydrogel on physiological

parameters of sunflower

Physiological

parameters

Methodology

Design : Split-plot Replications: 3

Main Treatments:

T1: Two irrigations (star bud and flowering)

T2: Three irrigations (star bud, flowering and seed filling)

T3: Four irrigations (vegetative, star bud, flowering and seed filling)

Sub Treatments:

T1: RDF

T2: RDF + K-Gel @ 2.5kg/ha - Spot application

T3: RDF + Pusa Hydrogel @ 2.5kg/ha – Spot application

T4: RDF + FYM@ 2.5t/ha – Spot application

T5: RDF + FYM@ 5t/ha – spreading

RDF = 60:60:30 Kg N:P2O5:K2O/ha through Urea, SSP and MOP

Plot size : 5.4 m × 4.8 m = 26 m2

Spacing : 60 X 30 cm

Season : Rabi

Date of sowing: October, 2019

Farm : Narkhoda Observations

Saving in irrigation water

Crop growth, yield and quality

Physiological parameters of plant water status

47

Project No. 104-15

Title Screening and identification of potential sources of tolerance to abiotic stresses and improved physiological efficiency in sesame

Principal Investigator

Ratna Kumar Pasala

Co-Investigators J. Jawahar Lal Praduman Yadav

PIMS Number IXX13052 Year of start 2016 Objectives 1. Physiological characterization of sesame germplasm and core

collection under drought condition 2. Impact of drought on oil content and fatty acid profile of

sesame selected lines


Objective 1: Physiological characterization of sesame germplasm and core collection

under drought condition


Ratna Kumar Pasala

Phenotypic characteristics Soil moisture content, Vapour pressure deficit

(VPD) Physiological parameters and canopy

temperatures Yield and components - capsule weight, seed

weight, test weight, multi and tri locular capsule. Functional and structural root characteristics Genotype with specific trait

Identification of potential source with physiological efficiency and better seed yielder in sesame core collection under water stress conditions

Experiment for quantification of genotypic variations for thermo tolerance

Identification of potential source with thermo tolerance

Methodology : Field experiment and pot culture experiment;

drought

imposition at flowering stage

Treatments/materials : Water stress and irrigation treatments/Sesame core

collection

No. of genotypes : 20 (selected from Core collection) + checks

Replications : 3

Plot size : 4 m

Spacing : 45 cm × 10 cm

Design : Strip plot

Season : Kharif & Rabi

Date of sowing : 3rd week of July 2019; 3rd week Jan 2020 approx.

Observations : Physiological parameters

Farm : Narkhoda

Land Requirement : 1500 Sq M


J. Jawahar Lal Pollen sterility in selected germplasm

and lines of core collection

Information on variation for

pollen sterility in selected core

collection under drought

48

Methodology : Anthers were fixed in 3:1 alcoholacetic acid (Jensen,

1962), and squashes were made using the

acetocarmine stain

technique of Snow (1963) method.

Treatments/materials : Sesame core collection

No. of genotypes : 15

Replications : 2

Spacing : 45 cm ×10 cm

Plot size : 2 m ×15 m

Design : Split plot

Season : Rabi

Date of sowing : 3rd week Jan 2019

Observations : Pollen sterility

Farm : Narkhoda

Objective 2: Impact of drought on oil content and fatty acid profile of sesame

selected lines


Praduman Yadav Oil content and Fatty Acid analysis

of selected core collections lines

Information on variation

for oil content and fatty

acid analysis among lines

for drought

Methodology : Oil content and fatty acid analysis

Treatments/materials : Core collection lines

No. of genotypes : 25

49

Project No. 104-16

Title Developing best management practices for organic soybean-sesame cropping system

Principal Investigator K. Ramesh Co-Investigators M.A.A. Qureshi

P. Duraimurugan Praduman Yadav

PIMS Number IXX14616 Year of start 2018 Objectives 1. To study the comparative performance of soybean and

sesame under organic, inorganic and integrated management conditions

2. To study the soil health under organic input conditions for Soybean-sesame cropping system

3. To develop non-chemical management strategies against storage insect pests of sesame

4. To evaluate the biochemical parameters of the released varieties under organic conditions


Objective 1: To study the comparative performance of soybean-sesame under

organic, inorganic and integrated management conditions


K. Ramesh To study the comparative performance of Soybean-sesame under organic, inorganic and integrated management conditions

Performance of Soybean-sesame under organic inputs vis-à-vis off farm inputs

M.A.A. Qureshi Analyzing the nutrient contents of organic inputs and imposing treatments

Nutrient profile of organic inputs

Praduman Yadav Analyzing the quality profile of oil Quality profiling of oils

Objective 2: To study the soil health under various nutrient input conditions for

Soybean-sesame cropping system


M.A.A. Qureshi Assessing the effect of nutrient management practices on soil health under soybean-sesame

Soil fertility changes under various input management conditions

Methodology

• Field experiments: : Soybean (kharif)

• Design : RBD

• Treatments (Nutrient management – 8)

M1. Control

M2. Organic : 100% RDF (FYM* + Rock phosphate) + PSB

M3. Organic : 100% RDF (Green/green leaf manure + Rock phosphate) + PSB

M4: Organic : 100% RDF (Castor oilcake + Rock phosphate) + PSB

M5. Organic : 100% RDF (Neem oilcake + Rock phosphate) + PSB

50

M6. Organic : 100% RDF (FYM + vermicompost + Goat manure) + Rock phosphate)

+ PSB

M7: Towards organic: 50% inorganic + 50% organic (through FYM + Rock

phosphate)

M8: Inorganic: 100% RDF (Urea, SSP/DAP, MOP)

*FYM will be applied as Trichoderma viride compost

• Design : RBD

• RDF : 20:60:20 kg NPK/ha (Soybean)

• Spacing : 45 x 5 cm (Soybean)

• Replications : 3

• Plot size : 9 × 5 m2

• Season : kharif (2019)

• Date of sowing : Soybean

(Jul first fortnight 2019)

• Farm : ICRISAT


Organic BMPs to be followed:

• Seed treatment with Trichoderma viride 4g/kg

• Application of Trichoderma viride compost

• Cultivation of green gram/black gram/sorghum/Daincha along the border

• Prophylactic spray with neem oil / neem formulations (1.5 l/ha at 15 days interval)

• “T” perch for predation of insect larvae

• Panchagavya spray @ 3% two times at 15 days interval

Work to be done/Observations

Soil nutrient availability at sowing and harvest of Soybean

Crop nutrient uptake (N, P and K)

Growth parameters of soybean at 60 DAS

Yield attributes at harvest

Biomass, seed yield and harvest index

Gross and net returns

Oil content and fatty acid composition

51

Activities/Experiments (2019-20)

Objective 1: Synthesis and functionalization of nanosystems based of Fe and Zn


K.S.V.P. Chandrika

& Balaji Gopalan

Combination of developed Fe and Zn

nanosystems

Combined iron and zinc

nanosystems

K.S.V.P. Chandrika

& Balaji Gopalan

Stabilization of developed

nanosystems

Stable combined iron and

zinc nanosystems

Methodology:

Core-Shell particle type

Double salt type

Observations:

Ratio of combining ability

Loading capacity by AAS

pH of nanosystems

Leaching behavior

Dispersion behavior

Objective 2: Characterization of developed Fe and Zn – nanosystems

Reaction confirmation

Fourier Transform infrared spectroscopy (FTIR)

X-ray diffraction (XRD)

Morphological properties

Scanning electron microscope (SEM)

Differential light scattering (DLS) and zeta potential

Thermal properties

Differential Scanning colorimeter (DSC)

Thermo gravimetric analysis (TGA)

Project No. 104-17

Title Fabrication of Fe and Zn nanosystems as efficient nutrient

sources

Principal

Investigator

K.S.V.P. Chandrika

Co-Investigators Md. A. Aziz Qureshi

Praduman Yadav

Co-operating Centre

PI

Dr. Balaji Gopalan (Chemistry), BITS Pilani, Hyderabad

Anupama Singh (Agril. Chemicals), ICAR-IARI, New Delhi


Year of start 2018

Objectives 1. Synthesis and functionalization of Fe and Zn nanosystems

2. Characterization of developed Fe and Zn - nanosystems

3. Evaluation of nutrient release, uptake and biochemical analysis

with optimized Fe and Zn- nanosystem for nutrient use

efficiency (Test crop: Soybean)

52


Experiment 1: Development of Conservation Agricultural Practices for castor based

cropping system under rainfed conditions Scientist(s) Activity 1 Deliverable(s) G. Suresh Maintenance of fixed plot of CA

experiment involving castor based cropping in Alfisols.

Layout, planning imposition of different CA practices viz. tillage methods, residue incorporation and castor based inter-cropping systems; execution of field experiments, growth and yield, crop residue biomass, crop residue cover, data collection, processing analysis, coordination and reporting

Growing castor and three castor based intercropping systems under rainfed conditions viz., castor + redgram (1:1); castor + greengram (1:3) and castor + groundnut (1:3) ; in 3 main plots (involving Conventional, reduced and zero tillage) in 12 treatment combina-tions in three replications

Field crop management, crop observations, sampling, coordina-tion, data compilation, analysis and reporting

Md. Aziz Qureshi

Analysis of soil physical, chemical and biological, microbiological properties; carbon sequestration potential of different treatments, soil profile studies; soil enzyme analysis; soil biota plant analysis nutrient uptake and soil nutrient balance studies

-

Treatment details Main Plot: Tillage and residue management: 3

CT-Conventional Tillage (one disc plough+ two cultivators +Rota tiller)

RT-Reduced tillage – one cultivator + one rota tiller (No disc plough)

ZT-Zero tillage- Cluster bean/Horse gram maintained as cover crop October onwards Sub Plot: Intercropping Systems 1. Sole castor

2. Castor+ redgram (for grain and cut in situ spread) (1:1)

3. Castor+ greengram (for grain and uprooted and insitu spread) (1:3)

4. Castor + groundnut (1:3)

Design: Split Plot; Replications: 3; Spacing: 120X 45-60cm for castor

Fertilizer: RDF

Project No. 104-18

Title Agronomic interventions for enhancing resource use efficiency in castor based cropping systems

Principal Investigator G. Suresh

Co-Investigators Md. Aziz A Qureshi

PIMS Number IXX14476 Year of start 2018 Objectives 1. To assess the impact of tillage practices and crop residues on

performance of castor based cropping systems. 2. Assessment of the impact of conservation agricultural

practices on carbon sequestration and changes in soil physical, chemical and biological properties

3. Economic and sustainability assessment of conservation agricultural practices

53

Plot size: Gross: 9.6 m x 5 m = 48 m2

Genotype: Castor (DCH-519), Redgram (Hanuma), Greengram (WGG-42), Groundnut (K-6)

Site: Fixed plot

Year of start: Kharif, 2018

Farm: Narkhoda Observations

• Initial and post-harvest soil pH, Organic Carbon, Total Carbon, Avail. N, Avail.

P2O5and Avail K2O

• Bulk density, Particle density Porosity and Water Stable Aggregates

• Soil enzymatic activity

• Soil moisture (%)

• Canopy temperature, Soil temperature

• Growth, Yield attributes and seed yield of main and intercrop

• Residue cover-Line transect method (Roughness coefficients)

• Quality parameters and yield

• Plant analysis of castor for total C, N, P and K.

• Economics (gross return, cost of cultivation, net returns and BC ratio)

• Weed flora, Weed density (No. / m2) (at 30, 60 days DAS)

• Crop residue biomass at crop harvest

• Monitoring weather and extreme weather events

*Note: castor stalks, leaf litter, will be in situ incorporate after picking. Experiment 2: Assessing the potential of castor stalks for in-situ and ex-situ

decomposition

In-situ- Shredding the biomass with a shredder and treating with efficient Microbial culture

for faster decomposition

Ex-situ-Composting by following standard procedures Experiment 3: Best Management Practices for maximum economic yield of castor in

Alfisols in large plots The following BMPs will be imposed in large plots

• Crop rotation; FYM @ 5t/ha

• Best hybrid DCH 519; ICH-66; DCH-177

• Seed hardening;

• Ridge and furrow method of planting

• Seed treatment with Bavistin, Trichoderma; Spot application n of Trichoderma with

seed

• Sowing by dibbling on ridges at 120cm x 45cm;

• Intercultivation through power threshers/tractor drwn blades

• Soil application of PSB/Azotobacter @2 kg/ha

• Pre-emergence herbicide application Pendimethalin @1 kg a.i/ha

• Thinning at 15DAS;

• Intercultivation through hand operated power tillers; Line weeding at 30DAS;

• Soil test and targeted yield based NPK, S and Zn SSNM (50% N as basal, 25% N at

30DAS + 25% N at primary + 10kg N/ha/picking); Sulphur at 30kg/ha (or P as SSP);

• Need based plant protection

• Integrated management of spodoptera, capsule borer; Pheramone trap monitoring

• Prophylactic bavistin spray to spikes at wet weather prediction against Botrytis,

• Harvesting at physiological maturity spike wise through secateurs

• Mechanical threshing Large plot non-replicated trial: 2000-2500 m2 in Alfisols at Narkoda farm

Season: Kharif 2019 Observations

Recording yield, cost of cultivation and economics

54

Project No. 104-19

Title Developing best management practices for sesame under rice-

sesame cropping system

Principal Investigator K. Ramesh (Agronomy)

Co-Investigators M.A.A. Qureshi (Soil Chemistry)

Praduman Yadav (Biochemistry)

Harisudan (Agronomy), TNAU, Vriddhachalam

Jogi Naidu (Agronomy), ANGRAU, Amdalavalasa

Mishra (Plant Breeding), OUAT, Mahisapet



Objective(s) To study the effect of tillage and nutrient doses on sesame crop

after rice


Objective 1: To study the effect of tillage and nutrient doses on sesame crop after rice


K. Ramesh To study the comparative

performance of sesame under tillage

and nutrient management conditions

Standardizing optimum tillage and

nutrient requirement for sesame

after rice at Hyderabad

M.A.A.

Qureshi

Analyzing the soil and nutrients of

rice soil for sesame

Optimum soil conditions for

sesame after rice at Hyderabad

Praduman

Yadav

Analyzing the quality profile of

sesame oil

Quality profiling of oils

(Hyderabad, Aduthurai, Amadala-

valasa and Mahisapet)

Harisudan To study the comparative





after rice at Aduthurai, TN

Jogi Naidu To study the comparative





after rice at Amadalavalasa, AP

Mishra To study the comparative





after rice at Mahisapet, Odisha

Methodology

• Field experiments: : Rice (Kharif)-sesame (January-February)

• Locations : 4 (Hyderabad, Aduthurai, Mahisapet, Amadalavalasa)

• Design : Split plot design

• Treatments

Main plots: Tillage management (3)

A1. Reduced tillage (Cultivator once)

A2: Conventional tillage (Cultivator twice with rotavator once)

A3. Zero tillage

Sub plot: Nutrient management (5)

B1: Control

B2: 25% RDF

B3: 50% RDF

B4: 75% RDF

B5: 100% RDF

55

• RDF : Hyderabad: 30:30:20 kg NPK/ha

Mahisapet : 50:30:20 kg NPK/ha

Adudurai : 35:23:23 kg NPK/ha

Amadalavalasa: 40:40:20 kg NPK/ha

• Replications : 3

• Plot size : 5 × 4 m2 (To be adjusted)

• Season : Rice (kharif 2019)-Sesame (late rabi/summer 2020)

• Date of sowing : Sesame (rabi/summer 2020) (depending on local

conditions)

• Farm : IIRR farm ICRIDAT for Hyderabad

o TRRI, Aduturai, Tamil Nadu

o ARS, Amadalavalasa, Andhra Pradesh

o OUAT, RRTTS, Mahisapet, Odisha

• Land area : 1500 m2

Work to be done/Observations

Nutrient availability in rice soil after harvest

Soil nutrient availability at sowing and harvest of Soybean

Crop nutrient uptake (N, P and K)

Growth parameters of Sesame at 60 DAS

Yield attributes at harvest

Biomass, seed yield and harvest index

Gross and net returns

Oil content and fatty acid composition

56


Objective 2: Field evaluation of formulations against Spodoptera litura in castor and

castor based intercropping system Experiment 1. Evaluation of field efficacy of Bt-127 WDG (67%) (pan drying) formulation

against S. litura Scientist(s) Type of Experiment Deliverable(s)

P. Duraimurugan & P. S. Vimala Devi

Field Field efficacy of Bt-127 (67%) WDG formulation against S. litura

Treatments: 8 1. DOR Bt-127 67% WDG @ 2g/l

2. DOR Bt-127 SC @ 3 ml/l

3. Delfin® (Btk check) @ 1.5 g/l

4. Profenophos 50EC@ 1 ml/l

5. Untreated control Design : RBD; Treatments : 5; Replications : 4 Cultivar : DCH-519 Season : Kharif 2019; Location : Rajendranagar farm Plot size : 4.8 m x 4.8 m (Land requirement: 800 m2); Spacing: 60 x 60 cm Date of sowing : Second week of August Methodology

Release of S. litura egg masses / gregarious stages of larvae only if required

S. litura larval population before and 3, 7 and 14 days after each spray and

per cent defoliation at 14 DAS

Phytotoxicity symptoms

Natural enemies

Seed yield and Economics Experiment 2. Shelf-life data generation of Bt127 67% WDG (pan drying) formulation as

per registration guidelines of CIB

Methodology

• Formulation will be stored in HDPE covers for 24 months at room temp.

• Data on shelf-life will be generated for the formulation at 3-monthly intervals (15-24

months) on the following parameters

– Heat viable spore count

– pH

– Bacterial and fungal contaminants

– Human pathogens

– Efficacy against second instar larvae of S. litura

Project No. 105-11

Title Development of water dispersible granular (WDG) formulation of Bacillus thuringiensis var. kurstaki for management of Spodoptera litura

Principal Investigator P. S. Vimala Devi Co-Investigators P. Duraimurugan

K.S.V.P. Chandrika Cooperating Centre Institute of Pesticide Formulation Technology (IPFT), Gurgaon PIMS Number IXX12567 Year of start 2015 Objectives 1. Development of WDG formulation (60-90% a.i.) with Bt-127

2. Field evaluation of formulations against Spodoptera litura in castor and castor based intercropping system

Scientist(s) Type of Experiment Deliverable(s)

P.S. Vimala Devi and K.S.V.P. Chandrika

Laboratory Shelf-life of the Bt-127 WDG formulation

57


Objective 1: To study the interaction of host, wilt pathogen and reniform nematode in

wilt disease complex

Scientist(s) Activity 1 Deliverable(s) B. Gayatri M. Santha Lakshmi P.

Interaction of F. oxysporum f. sp. ricini and reniform nematode in castor wilt disease complex in castor cultivars

Understanding the role of nematode in predisposing the plant to wilt

Material: Castor genotypesJI-35, 48-1

Methodology: Castor cultivars inoculated with F. oxysporum f. sp. ricini and reniform

nematode in different combinations as below.

• Un inoculated control

• Fungus alone

• Nematode alone

• Nematodes (20 DAS) followed by fungus (1 wk after nematode inoculation)

• Fungus (20 DAS) followed by nematodes (1 wk after fungus inoculation)

• Nematodes + Fungus (Simultaneously)

Réplication : 6 pots (4 plants/pot)

Observations: Wilt incidence (%), fungal load, nematode egg mass

Objective 2: To study variability in isolates of F. oxysporum f. sp. ricini and pathogen

population dynamics in sick plot


M. Santha Lakshmi

Prasad

Wilt reaction of castor genotypes to isolates of

Fusarium oxysporum f. sp. ricini under pot

culture conditions

Identification of

host differentials

Material: Castor genotypes comprised inbreds/ germplasm accessions/ parental lines along

with JI-35, 48-1

Methodology

Isolates of F. oxysporum f.sp. ricinifrom IIOR, Hyderabad, Palem, S.K. Nagar

Project No. 105-12

Title Screening and identification of durable sources of resistance to diseases of castor and deciphering the associated mechanisms

Principal Investigator M. Santha Lakshmi Prasad Co-Investigators B. Gayatri

Praduman Yadav

PIMS Number IXX12570 Year of start 2015-16 Objectives 1. To study the interactions of host, wilt pathogen and reniform

nematode in wilt disease complex 2. To study variability in isolates of F. oxysporum f. sp. ricini

and pathogen population dynamics in sick plot 3. To identify castor genotypes resistant to wilt disease 4. Management of wilt disease complex

58

Method: Sick pot method

Design: CRD; Replications: 3

Date of sowing: First fortnight of September, 2019 to January, 2020

Observations

Wilt incidence (%) at different intervals

Objective 3: To Identify castor genotypes resistant to wilt disease

Scientist(s) Activity -1 Deliverable(s)

M. Santha Lakshmi

Prasad

Evaluation of parental lines/

Experimental hybrids against wilt

under sick plot conditions


resistant parents /

experimental hybrids

Materials Parental lines / advanced breeding material /Experimental hybrids: 215+ checks, JI-35

(susceptible) and 48-1 (resistant) after every five entries

Methodology Design: ABD; Spacing: 60 × 45 cm;

Row length: 6.0 m / 4.5 m; Replications: 2/3

Season: Kharif

Date of sowing: I fortnight of July, 2019

Land requirement: 2800 m2 (sick plot)

Farm: Rajendranagar

Observations

Initial plant stand,

Wilt incidence (%) at 30, 60, 90, 120, 150 days after sowing.


M. Santha Lakshmi Prasad Screening castor germplasm

accessions against wilt disease


resistant sources

Materials

Germplasm accessions: 100 with 2 checks (susceptible-JI-35 & resistant-48-1) after

every five entries

Methodology

Design: ABD; Plot size: 1 row of 6 m length

Spacing: 60 × 45 cm; Season: Kharif

Date of sowing: I fortnight of July, 2019

Land requirement: 900 m2 (sick plot)

Farm: Rajendranagar

Observations

Initial plant stand;

Wilt incidence at 30 days interval up to 150 days after sowing.

59


Objective 1: To improve methodology for mass screening of castor genotypes for

resistance to sucking pests


P. Duraimurugan Development of in vivo and in vitro

methods to study mechanism of

resistance to whitefly in castor

Methodology to study

mechanism of resistance to

whitefly in castor

Materials

Genotypes : 6 (3 each resistant and susceptible genotypes)

Resistant genotypes: RG-2800, DPC-9, DCH-177

Susceptible genotypes: RG-2646, M-574, DCH-519

Methodology

Methods for evaluating whitefly resistance: 1. In vivo method (cage method)

2. In vitro method (leaf assay)

Design: Factorial CRD Replication: 4

Season: Rabi2019-20

Date of sowing: 2nd fortnight of December

Farm: Rajendranagar (Cage and laboratory experiments)

Observations: Artificial release of whitefly and studies on oviposition rate, development

periods and adult and juvenile survival rates (antixenosis and antibiosis

mechanism of resistance)


P. Duraimurugan Development of in vivo and in vitro

methods to study mechanism of

resistance to leafhopper in castor

Methodology to study

mechanism of resistance to

leafhopper in castor

Materials

Genotypes : 6 (3 each resistant and susceptible genotypes)

Resistant genotypes: RG-2661, RG-631, RG-3060

Susceptible genotypes: DPC-9, DCH-177, DCS-107

Methods for evaluating whitefly resistance: 1. In vivo method (cage method)

2. In vitro method (leaf assay)

Design: Factorial CRD Replication: 4

Project No. 105-14

Title Screening and identification of dependable sources of

resistance to insect pests of castor and deciphering the

associated mechanisms

Principal Investigator P. Duraimurugan

Co-Investigators -


Year of start 2017

Objectives 1. To improve methodology for mass screening of castor

genotypes for resistance to sucking pests

2. To identify castor genotypes resistant to major insect pests

3. To study mechanisms and inheritance pattern of resistance to

major insect pests

60

Season: Rabi2019-20

Date of sowing: 2nd fortnight of October

Farm: Rajendranagar (Cage and laboratory experiments)

Observations: Artificial release of leafhopper and studies on oviposition rate, development

periods and adult and juvenile survival rates (antixenosis and antibiosis

mechanism of resistance)

Objective 2: To identify castor genotypes resistant to major insect pests


P. Duraimurugan Screening of parental lines against

Capsule borer

Castor parental lines

resistant to capsule borer

Materials

Genotypes : 16 parental lines (P3-18NSp, P3-59NSp, P3-90NSp, P3-118NSp,

P3-124NSp, P3-98, P3-116, P3-290, P3-10, P3-16, MCI-8, JI-226, JI-227, JI-338,

JI-340, IPC-38) + Susceptible check (DCS-9) + Resistant check (48-1)

Methodology

Method: Infester row technique with DCS-9 (one infester row after every 2 rows in

sandwich method)

Design: RBD Replications: 2 Spacing: 90 x 60 cm

Season: Late Kharif

Date of sowing: IInd fortnight of August

Plot size: One row of 6m for each genotype


Farm: Rajendranagar

Observations

Capsule borer damage will be recorded both in the primary and secondary spikes


P. Duraimurugan Screening of parental lines against

sucking pests

Castor parental lines

resistant to sucking pests

Materials

Genotypes: 37 parental lines (27 monoecious lines: ICS-296 to ICS-322 and10

pistillate lines: IPC-22, IPC-30, IPC-31; IPC-40 to IPC-46)+ 4 Susceptible checks

(DPC-9, DCS-9, DCS-107, DCH-177)+ 2 Resistant checks (M-574, DCH 519)

Methodology

Method: Infester row technique with DPC-9 (one infester row after every 5 rows)

Design: Augmented Block Design

Season: Rabi 2019-20

Date of sowing: October IInd Fortnight

Plot size: 6m row

Spacing: 90 x 60 cm


Farm: Rajendranagar

Observations

Leafhopper population (No./3 leaves/plant), Hopperburn grade (0-4 scale); Thrips

population (on spike & top leaf) and Thrips damage score (0-4)

61

Project No. 105-15

Title Screening and identification of durable sources of resistance

to biotic stresses of sesame and deciphering the associated

mechanisms

Principal Investigator M. Santha Lakshmi Prasad

Co-Investigators P. Duraimurugan

H. H. Kumaraswamy



Objectives 1. Identification of resistant sources to root rot disease and

variations in pathogen infectivity

2. Development of rapid, reliable techniques for detection of

sesame phyllody and evaluation of germplasm and

breeding lines against phyllody

3. Development of an efficient mass-rearing, screening

techniques and identification of resistant sources to

leafhopper vector of phyllody, leaf webber and capsule

borer

4. Molecular characterization of sesame phyllody

phytoplasma and Macrophomina fungus


Objective 1: Identification of resistant sources to root rot disease and variations in

pathogen infectivity

Scientist(s) Activity -1 Deliverable(s)

M. Santha Lakshmi

Prasad

Evaluation of germplasm accessions /

advanced breeding material of sesame

against root rot

Identification of

resistant sources

to root rot

Material: Germplasm accessions/ advanced breeding material: 200 with checks (VRI-1,

GT-10) Method: Sick pot method;

Pots: 3 pots / cultivar; each pot: 10 plants

Place: Pot culture studies and Laboratory, Rajendranagar

Observations Initial plant stand; Root rot incidence (%)

Sending seed material of moderately resistant sesame genotypes to Vridhachalam,

Tamil Nadu. Screening of genotypes against root rot under sick plot conditions for

confirmation. Objective 2: Development of rapid, reliable techniques for detection of sesame phyllody

and evaluation of germplasm and breeding lines against phyllody


M. Santha Lakshmi P.

P. Duraimurugan

Transmission studies of phyllody

through different methods.

Selection of suitable

transmission method for

phyllody screening

Methods: Transmission through sap, grafting, cuscuta, leafhopper

62

Materials:

Sesame genotypes: 2;

Leafhopper studies: Sunhemp, Peri winkle (Vinca rosea)

Pots: 5 pots / cultivar; Each pot: 3 plants

Place: Pot culture studies, Laboratory, Rajendranagar

Observations

Phyllody incidence at regular intervals


M. Santha Lakshmi P.

P. Duraimurugan

H. H. Kumara Swamy

Evaluation of germplasm

accessions/ advanced breeding

material under field conditions

Occurrence of

phyllody, leaf hopper

on genotypes

Materials: Germplasm accessions/ advanced breeding material: 100 with checks; GT-10;

SL-II /RJR-170

Methodology

Sowing: July II FN; Plot size: 2 rows of 3 m for each genotype

Replications: 2; Spacing: 45 x 10 cm

Farm: Rajendranagar; Land requirement: 1200 m2

Observations

Phyllody incidence; leafhopper population; nested PCR in genotypes with low phyllody

incidence

Objective 3: Development of an efficient mass-rearing, screening techniques and

identification of resistant sources to leafhopper (vector of phyllody),

leafwebber & capsule borer (Antigastra)


P. Duraimurugan Validation of methodology for mass screening

of sesame genotypes for resistance to

leafwebber & capsule borer(Antigastra) under

field conditions

Validation of

methodology for

mass screening

against Antigastra

Method of screening: 1.Sowing during July II FN + planting one infester row of Prachi after

2 rows of test entries;

2. Sowing during July II FN without infester row

Treatments: 50 genotypes + Susceptible check (TC-25) + Resistant checks

(Shwetha, GT-10)

Date of sowing: July II FN; Plot size: One row of 3m for each genotype

Spacing: 45 x 10 cm; Land requirement: 500 m2 (Rajendranagar)

Design: Strip plot Replications: 2

Observations

Leaf, flower and pod damage will be recorded from five selected plants and damage will be

assessed on 1 to 9 score.

63


Objective 2: Mass production and evaluation of promising EPN strains on Spodoptera

litura and Liriomyza trifolii in vivo and in vitro Scientist(s) Activity-1 Deliverable(s) B. Gayatri

Mass multiplication of virulent EPN strains under In vivo and In vitro conditions

Mass production technology for EPN multiplication

Methodology:

a) Mass multiplication of EPNs (In vivo method):

Late instar larvae of Galleria melonella will be used.

Infected with different concentrations (30, 60, 90, 120, 150 and 200 IJs/larva)

of Heterorhabditis indica and H. bacteriophora.

Cadavers placed on modified white’s trap for IJ (infective juvenile) emergence

Emerged IJs were collected and counted separately for each concentration of

each EPN

b) Mass multiplication of EPNs (In vitro method):

Using different artificial media.

Media with protein source for bacteria and sterol source for nematode

Single bacterial colony will be inoculated into the media and left for its

multiplication for 48 hours

After 48 hours, sterile IJs will be inoculated into the bottle.

Nematodes will be harvested after 2-3 weeks.

Replications: 3 per media per EPN species.

Observations: Total IJ production

In-vivo evaluation

Pot experiment will be conducted during Kharif 2019 (Rajendranagar)

Treatments:

1. Heterorhabditis indica (100, 200, 300 IJs/larva)

2. Heterorhabditis bacteriophora (100, 200, 300 IJs/larva)

3. *Poison baiting with EPNs (Heterorhabditis indica & Heterorhabditis

bacteriophora)

4. *Poison baiting with insecticides (Novaluron/Chlorpyriphos)

5. Untreated control

*Treatments for S. litura only

Cultivars used: DCH-519 (for S. litura); DCH-177 (for L. trifolii)

Host insects: Late instar larvae of S. litura and L. trifolii

Design: Factorial CRD; Replications: 4

Date of sowing: First fortnight of June

Method of application of EPNs: Soil application

Observations: Insect mortality at different days after application

Project No. 105-16 Title Exploiting the bioefficacy of entomopathogenic nematodes

against Tobacco caterpillar (Spodoptera litura) and Serpentine leaf miner (Liriomyza trifolii) in oilseed crops

Principal Investigator B. Gayatri Co-Investigators P. Duraimurugan Cooperating Centre PI Sunanda, B.S. (Nematology), NIPHM PIMS Number IXX13582 Year of start 2017 Objectives 1. Collection of EPN strains and testing the virulence on

different life stages of Spodoptera litura and Liriomyza trifolii

2. Mass production and evaluation of promising EPN strains on Spodoptera litura and Liriomyza trifolii in vivo and in vitro

Scientist(s) Activity-1 Deliverable(s) B. Gayatri P. Duraimurugan

In vivo evaluation of virulent EPN strains

Promising EPN strain

64

Objectives

1. Determination of nutritional parameters in safflower seed cake

2. Preparation and characterization of the protein hydrolysates

3. Evaluation of the efficacy of safflower seed protein hydrolysates in commercial

broiler and backyard chicken varieties reared under heat stress and winter

conditions.

Experiments/Activities (objective-wise) Objective 3: Evaluation of the efficacy of safflower seed protein hydrolysates (SSPH)

in commercial broiler and backyard chicken varieties reared under heat

stress and winter conditions

Methodology Treatments-6, Replications-10, Dose- Dose-g/Ton- 50 and 100% replacement of soybean

meal with safflower meal

1. Growth Parameters

a. Body weight gain, Feed intake, Feed efficiency, Livability

2. Oxidative stress parameters

a. Lipid peroxidation, Glutathione peroxidase, Super oxide dismutase, Catalase

b. Antioxidant activity- DPPH, ABTS, FRAP, ORAC and CUPRAC

3. Slaughter parameters-If significant variations will be found in growth parameters


Hari Shankar, ICMR-

NIN

Praduman Yadav

To study the effect of safflower proteins

on the reproductive performance and

nutritional efficacy of WNIN rats

Changes in reproduce-

tive performance and

nutritional efficacy Methodology:

Animal: 60 male and 60 female WNIN rats

• Group-1: Control diet

• Group-2: Safflower protein concentrates(SPCs)

• Group-3: Safflower Protein Isolates (SPIs)

• Group-4: Safflower Protein Hydrolysates (SPHs) Observations

• Weekly body weights and daily food intake

• Nutrient metabolites, net protein utilization and feed efficiency ratio

• Serum analysis (Serum glucose, cholesterol and triglyceride levels)

• Lipid peroxidation and histopathological studies

Project No. 106-2

Title Production and characterization of protein hydrolysates from safflower seed and validation of their utility in animal nutrition

Principal Investigator Praduman Yadav

Co-investigators K.S.V.P. Chandrika M. Santha Laxmi Prasad

Co-operating Centre PI S.V. Rama Rao (Principal Scientist, Avian Nutrition, ICAR-DPR, Hyderabad), Hari Shankar (ICMR-NIN, Hyderabad)

PIMS Number IXX13051 Year of start 2016


S.V. Rama Rao

Praduman Yadav

Evaluation of the efficacy of safflower

defatted seed in Vanaraja chicken diet

Changes in growth

response parameters

65

• Reproductive performance (Reproductive hormone profile Mating of the rats


Objective 1: To develop structure and content of Oilseeds Knowledge Management

Portal (OKMP) comprising General information System, Research

Information Systems, Extension Information System, Market

Information System and Farming Information System

Scientist(s) Activity 2: Research Domain Deliverable(s)

P. Madhuri 1. Designing and developing structure for safflower

knowledge portal

Research

related

information on

oilseeds Ch. Sarada 2. Digitization and uploading data related to safflower

(attributes like area, production, productivity etc., -

region wise)

N. Mukta 3. Compiling the information pertaining to safflower

Research in India

P. Madhuri

TIO

4. Digitization of information w.r.t safflower AICRP

centres

Ch. Sarada 5. Developing Directory of Oilseeds Research

Organizations

G.D. Satish

Kumar

6. Success Stories

Scientist(s) Activity 5: Extension Domain Deliverable(s)

P.Padmavathi

G.D. Satish Kumar

1. POPS on Safflower Extension materials

P.Padmavathi 2. Contingency Plans

Project No. 107-16

Title ICT mediated knowledge management and dissemination in

different oilseed crops

Principal

Investigator

P. Madhuri

Co-Investigators Ch. Sarada

N. Mukta

G.D. Satish Kumar

P.Padmavathi

S.V. Ramana Rao

G. Raghunath

K. Anjani

S.N. Sudhakara Babu

P. Kadirvel

B. Usha Kiran

P. Satya Srinivas

R.D.Prasad

Praduman Yadav

H.P. Meena


Year of start 2015

Objectives 1. To develop structure and content of Oilseeds Knowledge

Management Portal (OKMP) comprising General information

System, Research Information Systems, Extension Information

System, Market Information System and Farming Information

System

66

G.D. Satish Kumar 3. FAQs

P. Madhuri 4. Photo Library

G.D. Satish Kumar 5. Details of Nearest Extension Officer

G.D. Satish Kumar 6. FLDS

Scientist(s) Activity 3: Farmers Domain Deliverable(s)

S.V. Ramana Rao 1. Government Schemes Information on

farmers, seeds and

schemes G.D. Satish Kumar 2. Farmers Innovations

Scientist(s) Activity 4: Service Domain Deliverable(s)

S.V. Ramana Rao

G. Raghunath

1. Trade Know How Information on

product and market

services P. Madhuri

S.V. Ramana Rao

2. Mandi Prices

K. Anjani 3. Varieties/Hybrids Recommended

(State wise)

Scientist(s) Activity 1: General Information Deliverable(s)

N.Mukta 1. About Safflower General crop

related information N.Mukta 2. Safflower Germplasm Resources

K.Anjani 3. Safflower Hybrids and Varieties (With

Characters and Photos)

S.N. Sudhakara Babu

K.Anjani

4. Safflower seed production

K.Anjani

P. Kadirvel

5. Safflower Breeding Research

(Compilation with Photos)

B. Usha Kiran

P. Kadirvel

6. Safflower Biotechnology Research

P.Padmavathi 7. Safflower Production Technologies

P. Satya Srinivas 8. Safflower Pests

R.D.Prasad 9. Safflower Diseases

Praduman Yadav 10. Safflower Quality aspects

S.N. Sudhakara Babu 11. Seed Availability

TIO

G. Raghunath

12. Safflower Research Publication (National

and International / Only from IIOR and

AICRP)

P. Madhuri

G. Raghunath

13. News and Events

N.Mukta

Praduman Yadav

14. Uses of Safflower

67

Objectives

1. To quantify the impact of the on-farm technologies demonstrated

2. To study the adoption behaviour of the oilseed farmers

3. To prepare a digital database/inventory of the on-farm technologies demonstrated

Activities/Experiments (objective-wise)

Objective 1: To quantify the impact of the on-farm technologies demonstrated


S.V.Ramana

Rao

Primary data collection and

analysis of sample households

Safflower: Solapur and Latur

districts of Maharashtra

Quantifies the impact of the proven

technologies on yield /monetary returns.

Provides feedback for technology

refinement

Methodology

• Data shall be collected from the centres where FLD’s were conducted from 2015-

16 to 2018-19

• Preparation of schedules

• Primary data collection through Farm Household survey method (25 farmers

each).

• Total sample size: 50

• Period of survey: November, January, February

Data to be collected

• Demographic characteristics

• Input use pattern (cost and qty) of purchased inputs, labour (human, bullock,

Tractor, machinery)

• Production management practices

• Cropping pattern

• Output marketing

• Constraints confronted in production and marketing

Objective 2: To study the adoption behaviour of the oilseed farmers


G.D.Satish

Kumar

To develop an inventory of technologies demonstrated

under FLD’s

Field survey to assess the adoption/continuance/

discontinuance of the technologies

An adoption

index would

be developed

Project Number 107-17

Title On-farm demonstrations of improved

technologies and impact assessment of the

adoption

Principal Investigator S.V.Ramana Rao

Co-investigators G.D.Satish Kumar

P.Madhuri



(Extended by one year to 2019-20 in Rabi,

IRC 2019)

68

Methodology

• Data shall be collected from the centres where FLD’s were conducted from 2015-

16 to 2018-19

• Preparation of schedules

• Primary data collection through Farm Household survey method (25 farmers

each). Total sample size: 175

• Period of survey: November, January, February

Data to be collected

• Demographic characteristics

• Input use pattern

• Technologies demonstrated (variety/ hybrid, seed rate, spacing, thinning, RDF,

Need based plant protection

• Productivity and income

Objective 3: To prepare a digital database / inventory of the on-farm technologies

demonstrated


G.D.Satish

Kumar

To develop an inventory of technologies

demonstrated under FLD’s

Ready reckoner of

technologies demonstrated

P.Madhuri Document and digitize the inventory of

technologies demonstrated under FLD

69

Project No. 107-18

Title Impact assessment of hybrids/ varieties of IIOR mandated

crops in varied agro ecological regions of India

Principal Investigator S.V.Ramana Rao

Co-Investigators C. Sarada

Cooperating Centre PI K.P.Thakar, Professor and Head, Dept. of Ag. Econ, , C. P.

College of Agriculture, SDAU, Sardarkrushinagar)



Objective(s) 1. To examine the costs, returns and profitability of oilseeds

based production systems

2. To decompose the change in production for measuring the

contribution of technology

3. To identify the impact of the technology to change in income


Objective 1: To examine the costs, returns and profitability of oilseeds based production

systems (castor based)


S.V.Ramana

Rao

K.P.Thakar

Primary data collection of sample households

from Banskanta, Sabarkanta and Mehsana

districts of Gujarat

Analysis of the primary data to assess the

economics of castor based production system

Provides farm level inter-

ventions for increasing the

productivity through

bridging the yield gaps

Methodology

a) Primary data collection through Farm Household survey method

b) Three districts from Gujarat (Banskanta, Sabarkanta and Mehsana)shall be purposively

selected for the study

c) From each district two tehsils would be selected in consultation with SDAU/ seed

industry

d) From each tehsil two villages shall be selected based on the coverage of GCH-7

e) From each village, 10 farmers would be selected for the study

Thus the sample per district would comprise of 40 households. The total sample for the state

would be 120.

Data shall be collected on the following observations:

Demographic characteristics

Input use pattern (cost and qty) of purchased inputs, labour (human, bullock, Tractor,

machinery)

Production management practices

Cropping pattern

Output marketing

Constraints confronted in production and marketing

Analysis of data for assessing the farm level

Costs, returns and profitability (SVRR/KPT)

70

Objective 2: To decompose the change in production for measuring the contribution

of technology


C.Sarada

S.V.Ramana

Rao

Quantify the contribution of technology through

decomposition of area, yield and interaction of

area and yield effect.

Assess the performance of castor (in Gujarat) vis-

a-vis competing crops and for Replacement / area

increase of the crop after GCH-7

Enables for identify-

cation of castor area

on AESR approach

that warrants inter-

ventions for enhancing

the productivity.

C.Sarada Assessing the Kinked growth

Secondary data –Collection

Collection of time-series data on A,P,Y of castor and competing crops (GJ state from

1995-96 onwards)

District-wise data on A,P and Y for respective crop and the competing crops from (GJ

state / important castor growing districts from 1995-96 onwards) (CS/SVRR)

Prices for castor and the competing crops (1995-96 onwards) (SVRR/ CS)

Analysis of data with respect to

Growth and instability analysis (CS)

Kinked growth analysis (CS)

Decomposition analysis of change in production (CS /SVRR)

Objective 3: To identify the impact of the technology to change in income


S.V.Ramana Rao

K.P.Thakar

Examine the contribution of SAU’s/ private seed

industry, the subsidy component (if any) for

estimating the impact of the technology (GCH-7)

in the Economic surplus Model

Quantifies the

economic impact

of GCH-7 techno-

logy in Gujarat

C.Sarada Collection of historical data from published

sources on the input-output matrix of castor and

for the competing crops

Secondary data –Collection

Collection of data on Research costs, Extension Costs, seed production costs, subsidy

cost, and other associated costs Source: SDAU/ private seed sector/Govt. agencies

(SVRR/KPT)

Collection of secondary data (Cost and quantity of inputs (monetary and non-

monetary); productivity and gross and net returns) on competing crops of castor from

1995 onwards from published reports (CACP/SDAU/Govt. of Gujarat/ other

published sources) (CS)

Collection of secondary data (Cost and quantity of inputs (monetary and non-

monetary); productivity and gross and net returns) of castor from 1995 onwards from

published reports (CACP/SDAU/Govt. of Gujarat/ other published sources) (SVRR)

Analysis of data with respect to

Assess the impact of GCH-7 (SVRR/KPT/CS)

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Objective-1: Creation of time series of both crop yield and meteorological data

K. Alivelu To collect and compile data on state wise district wise weather parameters and creation of time series of meteorological data

Long term time series data of state and district wise weather parameters

C. Sarada To collect and compile data on state wise district wise yield of oilseed crops (Castor, Sunflower Safflower) and Creation of time series of oilseeds yield data

Long term time series data of yield of castor, sunflower and castor.

Objective-2 : Determine the statistical relationship between Weather parameters and yield K. Alivelu Estimating magnitude and statistical

significance of trend in climatic data of state and district wise

Trends in weather data will be investigated

C. Sarada Statistical relationship with weather variables and yield of oilseeds

Correlation with yield of oilseeds & weather variables

Objective-3: Develop prediction models to predict yield from climate variables over the area of study K. Alivelu C.Sarada

Development of yield Prediction models Different prediction models

K. Alivelu C.Sarada

Selection of best Prediction model based on evaluation parameters

Best prediction model

Objective-4: Validation of the developed models

K. Alivelu C.Sarada

Assess the performance of the best model for accuracy

Accuracy of the model

K. Alivelu C.Sarada

Estimation and comparison of forecasts with actual yield

Percentage error for forecast yield against actual yield

Methodology Meteorological data will be used from local observations and also will be obtained from

the National Data Centre, IMD, Pune. Crop data at district level will be collected from

the DAC&FW, as well as from State Department of Agriculture. The districts

contributing higher production of the State will be identified for development of yield

forecast models of respective districts.

Man Kendall and Sen’s slope estimator nonparametric test will be used for studying

trend in climate data.

Changes in climatic variable will be utilized for relating castor yield.

Appropriate models based on the behavior of the data will be used for developing

prediction models.

Different evaluation parameters will be used for selecting best model

Validation of the models will be carried out using the yield data and weather parameters

of the given district

Project No. 107-19

Title Development of models to predict yield responses to climate change in Oilseed crops

Principal Investigator K. Alivelu

Co-Investigators C. Sarada PIMS Number IXX14482 Year of start 2018 Objective(s) 1. Creation of time series of both crop yield and

meteorological data 2. Determine the statistical relationship between weather

parameters and yield 3. Develop prediction models to predict yield from climate

variables over the area of study. 4. Validation of the developed models 5. Development of Decision Support System

72


Scientist(s) Activity-1

Jawahar Lal J. Backcrossing of male sterile BC5F1 plants with recurrent parent

Materials: BC5F1(ISMB-2 x GT-10) F1x GT-10 -1-1-1-2-1-1

BC5F1(ISMB-3 x GT-10) F1x GT-10-1-1-1-2-1-1

Methodology

Field experiment

Season: Kharif


Farm: Narkhoda


Jawahar Lal J. Backcrossing of male sterile BC3F1 plants with recurrent parent

Material: BC3F1- (ISMB-7 x GT-10) F1 X GT-10-1-1-1-2

Methodology

Field experiment

Season: Kharif


Farm: Narkhoda


Jawahar Lal J. Backcrossing of male sterile BC1F1 with recurrent parent (Gujarat

collection)

Material: BC1F1-(G-3 x TKG-22) F1 x TKG-22-1-1-1

Methodology

Field experiment

Season: Kharif


Farm: Narkhoda

Project No. 108-1

Title Development of stable Cytoplasmic Genetic Male Sterile

(CGMS) system in sesame through wide hybridization

Principal

Investigator

Jawahar Lal Jatothu

Co-Investigators -


Year of start 2013

Objective(s) 1. To develop stable CMS system in sesame

73

Experiments/Activities (2019-20) Objective 1: To evaluate and characterization of germplasm

Scientist(s) Activity-1A Deliverables

Jawahar Lal. J.

Ramya. K.T.

Characterization and evaluation of sesame

germplasm accessions

Identification of trait

specific genotypes

Materials: 350+ 3 checks Shweta til, TKG-22 and GT-10 Design: Alpha lattice, Replications: 2

Row 2; row length: 4 m. Spacing: 45 x 10 cm

Sowing seasons: Kharif-2019, Summer-2020

Sowing: Ist fortnight of July -2019, II-fortnight October- 2019 (150 high oil accessions),

II-fortnight January-2020 Observations Plant height (cm)

Number of branches/plant

Number of capsules/plant

Number of seeds/capsule

Seed yield/plant (g)

Seed yield /plot (g)

Oil content (%) Objective 2: To develop agronomically superior, high yielding breeding lines


Ramya K.T. Raising F1s Generation advancement No. of F1s: 8 multi-parent hybrids developed using 13 parents (GT-2 XHT-1)/(IC-132201 X IC-500472)//(RT-351 X TKG-22)/IC-96160 X 204618)

(IC-96227 X IC-132201)/IC-96160 X IC-204618)//(TSS-6 X VRI-3)/(HIMA X SHWETA

TIL)

(IC-132201 X IC-204613)/(VRI-3 X HT-1)//(GT-2 X E-8)/(PHULE TIL X RT-351)

(PHULE TIL X RT-351)/GT-2 X E-8)//(IC-204613 X IC-500473)/IC-132201 X IC-96160)

(IC-96160 X IC-96227)/(IC-204618 X IC-500472)//(RT-351 X TKG-22)/(IC-132201 X IC-

204613)

(GT-2 X HT-1)/(RT-351 X TKG-22)//(IC-132201 X IC-96160)/(PHULE TIL X VRI-3)

(HT-1 X RT-351)/(GT-2 X TKG-22)//(HIMA X TSS-6)/(RAJESHWARI X E-8)

(RAJESHWARI X E-8)/(TSS-6 X VRI-3)//(IC-204613 X IC-500473)/(IC-96227 X IC-

204618)

Project No. 108-2

Title Exploitation of inter and intra specific genetic resources for

development of elite breeding lines in sesame

Principal Investigator K.T. Ramya

Co-Investigators Jawahar Lal Jatothu


Year of start 2017

Objectives 1. To evaluate and characterization of germplasm

2. To develop agronomically superior, high yielding breeding

lines

3. To evaluate and identify newly developed superior

genotypes for different agro climatic conditions

74

Oil content and yield characters of the parents used in developing multi parent F1 hybrid

Variety/Accession Yield (kg/ha) Oil content (%)

E-8 500 46-48

GT-2 750-800 48-52

HT-1 700-750 48-50

HIMA 800-850 48-50

RT-351 700-800 48-51

SHWETA TIL 750-800 50-52

TSS-6 750-800 50-54

VRI-3 800-1000 48-50

IC-132201 44

IC-500472 45

IC-96160 50

IC-204618 47

IC-204613 45

IC-96227 45

No of rows: 2; row length: 4 m. Spacing: 45 x 15 cm

Land requirement: 150 sq. m.

Date of Sowing: 1 fortnight August-2018

Raising of F2

Material: 5 crosses

(IC-96227 x IC-96160) x (RAJESHWARI x VRI-3)

(IC-132201 x IC-96160) x PHULE TIL x VRI-3)

(PHULE TIL x E-8) x (IC-204613 x IC-96160)

(PHULE TIL x RT-351) x (IC-500472 x IC-96227)

IC-204300 x IC-204444

Population: 2000, Number of rows: 100; row length: 4 m. Spacing: 45 x 10 cm

Land required: 2000 sq.m.

Sowing: Ist-fortnight of July-2019, IInd-fortnight January-2020

Generation advancement

Scientist(s) Activity-2B Deliverables

Ramya K.T. J. Jawahar Lal

Raising F4-7 crosses F5-4 crosses F6-4 crosses

Single plant selected for desirable traits

F4: IS-49-1A x RT-346, CT-55 x CT-57, EC-30344-1B x TKG-22, S-195 x CT-57, H-33 x

GOWRI

CT-36 x TMV-7, DSTA-1-A x VRI-2

F5: NIRMALA x IS-427, NIRMALA x S-0479, NIRMALA x JHA-1610-2, GRT-8609 x

HT-2

F6: RT-346 x S-0449, SI-349 x DS-5, N-32 x RT-127, IS-846-A x VRI-3

No. of rows: 2; row length: 4 m. Spacing: 45 x 10 cm

Land requirement: 2000 sq. m.

Date of sowing: II-fortnight July-2019 and I-fortnight August -2019

Generation followed to Summer-2020

Farm: Narkhoda

75

Observations

Plant height (cm)



Capsule length (cm)




Oil content (%)

1000 seed weight (g)

Uniform maturity

Low shattering or No shattering

Objective 3: To evaluate and identify newly developed superior genotypes for different

agro climatic conditions


Jawahar Lal.J.

Ramya K.T.

Preliminary yield evaluation Identification of high

yielding entries

Materials

SET1: 30 lines

SET3: 20 lines

Methodology

Experimental design: RBD, 3 replications with 3 checks

Number of rows: 3

Row length: 4 m


Sowing seasons: Kharif -2019, Summer-2020

Date of sowing: II fortnight of July, 2019, II fortnight January-2020

Land area required: 450 m2 per sowing

Farm: Narkhoda

Observations:

Plant height (cm)



Capsule length (cm)




Oil content (%)

1000 seed weight (g)

Scientist(s) Activity-3B Deliverables

Ramya K.T.

Jawahar Lal.J

Evaluation of released varieties Estimation of genetic gain

76

Materials

50 Released varieties along with 3 national checks (TC-25, TKG-22, GT-10),

1 standard check (RAMA) and 4 zonal checks (RT-46, RT-54, PRAGATI, JTS-8,

CO-1)

Methodology

Experimental design: RBD, 3 replications with 3 checks

Number of rows: 3

Row length: 4 m


Sowing seasons: Kharif -2019, Summer-2020

Date of sowing: IInd fortnight of July 2019, II fortnight January-2020

Land area required: 1000 m2

Farm: Rajendranagar

Observations

Plant height (cm), Number of branches/plant, Number of capsules/plant, Capsule

length (cm), Number of seeds/capsule, Seed yield/plant (g), Seed yield /plot (g), Oil

content (%), 1000 seed weight (g)

Scientist(s) Activity-3C Deliverables

Ramya K.T.

J.Jawahar Lal

Seed production Nomination to AICRP

Testing

Material: Set-I: 02 entries

Set-II: 5 best entries from preliminary yield trial (Activity 3A)

Number of rows: 50 for each genotype

Row length: 4 m


Date of sowing: Set I: Ist fortnight August, 2019

Set-II: IInd fort night Jan-2020

Land area required: 1200 m2

Farm: Narkhoda

77


Objective 1: To identify a working set of SSR markers in sesame


Kumaraswamy, H.H. Screening/optimization of amplification

condition for a set of 100 SSR markers

Additional working

set of SSR markers

Materials : Different genotypes of Indian sesame

Methodology: PCR & Agarose gel electrophoresis (3%)

Observations : Variation in amplicon sizes on agarose gel


Kumaraswamy, H.H. Assigning of linkage group to amplified SSR

markers using in silico approach:

Computational analysis of 100 SSRs

Data on genomic

distribution of SSR

markers

Methodology : Computational analysis

Observations : BLAST hits and presence of SSRs

Objective 2: To develop skeleton SSR linkage map in sesame


Kumaraswamy, H.H. Crossing of contrasting parents F1 seeds

Materials : Four contrasting parents

Methodology : Field experiment

Season : Kharif

Date of sowing : 2nd fortnight of June 2019



Observations : Seed setting of crossed flowers


Kumaraswamy, H.H. Parental polymorphism survey

using SSRs

Polymorphic SSR markers

between parents used for

crossing Materials : DNA from parents used in crosses

Methodology : Lab experiment

Observations : PCR amplicons polymorphic between parents used in cross

Project No. 108-3

Title Development of genetic and genomic resources and

identification of gene/marker for different agronomic traits in

sesame

Principal Investigator Kumaraswamy, H.H.

Co-Investigators M. Santha Lakshmi Prasad

P. Duraimurugan

P. Ratna Kumar


Year of start 2017

Objectives 1. To identify a working set of SSR markers in sesame

2. To develop skeleton SSR linkage map in sesame

3. To Identify and stabilize genotypes for various agronomic traits

78


Kumaraswamy, H.H. Selfing of F1 individuals F2 seeds

Materials : Hybrids of 4 crosses along with parents


Season : Kharif

Date of sowing : 2nd fortnight of September 2019



Observations : Phenotypic comparison with parents


Kumaraswamy, H.H. Hybrid confirmation using

polymorphic SSR loci

Molecular data on

hybridity of F1

generation


Observations : Heterozygosity for marker loci


Kumaraswamy, H.H. Genotyping of F2 individuals Data on segregation and

recombination of SSR loci

Materials : F2 segregating population


Observations : Allelic information for each SSR loci



Kumaraswamy, H.H. Phenotyping of F2

individuals for various

agronomic traits

Data on segregation and

recombination of various

agronomic traits

Materials : F2 segregating population


Season : Summer

Date of sowing :2nd fortnight of January 2019

Land requirement : 300 m2


Observations : Segregating agro-morphological traits

79


Objective 1: To collect, characterize, multiply, maintenance and evaluate germplasm

lines for major agronomic traits


Pushpa H.D. A. Vishnuvardhan Reddy

Collection of new niger germplasm lines from farmers field

A set of germplasm lines with high oil, high yield and low self-incompatible

Pushpa H.D. Seed multiplication of existing 200 niger germplasm

Seed multiplication of niger germplasm for agronomic traits

Treatment : 200 Niger germplasm

Location : Narkhoda

Plot Size : 2 rows of 4 m length

Spacing : 30 cm x 10 cm

Season : Kharif-2019

Sowing : 1st fortnight of July

Objective 2: To develop improved breeding populations for high yielding, high oil and

high autogamy potential


Dr. Pushpa H.D. Dr. A. Vishnuvardhan Reddy

Random mating of RMC I population to develop RMC II

A set of diverse niger population for seed yield and yield attributing traits and for seed oil content

Development of random mating cycle II

Treatment : Bulked 300g niger seed from best performing lines

Location : Rajendranagar





Pushpa H.D. A. Vishnuvardhan Reddy

Generation of polycross cycle I

A set of recombinant progenies of niger for major agronomic traits

Generation of polycross cycle I

Treatment : 12 Niger genotypes

Location : Narkhoda

Plot Size : 10 rows of 4m length




Genotypes : JNC-1, JNC-6, JNC-28, JNC-30, JNS-9, JNS-28, UTKAL-150,

DEMOLi, DNS-4, BNS-10, IGPN-2004-1, IGPN-8004

Project No. 109-1

Title Exploitation of plant genetic resources for development of

improved breeding populations in niger

Principal Investigator H.D. Pushpa

Co-Investigators A. Vishnuvardhan Reddy


Year of start 2017

Objectives 1. To collect, characterize, multiply, maintenance and evaluate

germplasm lines for major agronomic traits

2. To develop improved breeding populations for high yielding,

high oil and high autogamy potential

80

EXTERNALLY FUNDED PROJECTS

Title Mass production of Bacillus thuringiensis (Bt) and Beauveria

bassiana, formulation as oil based suspension concentrates

singly and in combination and field evaluation

Principal Investigator P. S. Vimala Devi

Co-investigator(s) P. Duraimurugan

K.S.V.P. Chandrika

Funding source ICAR Network Project-AMAAS

Total Amount

Sanctioned

-

Fund for the year Rs. 7,14,868/-

Year of start 2014

Year of completion 2020

PIMS No. OXX04311

Objectives 1. Mass production of Bt and B. bassiana

2. Milling of Bt to fine particle size of 5-20 μm and formulation

as a suspension concentrate

3. Microencapsulation of B. bassiana conidia through spray

drying with humic acid

4. Formulation of Bt singly and Bt + B. bassiana in combination

as stable suspension concentrates

5. Formulation of microencapsulated conidia of B. bassiana as a

suspension concentrate

6. Large scale field evaluation on oilseed crops as components of

IPM against major lepidopteran pests-Helicoverpaarmigera,

Spodoptera litura, Achaea janataand Conogethes (Dichocrocis)

punctiferalis

Experiments/Activities (2019-20) Experiment 1. Mass production of Bt127 through SSF and development of suspension

concentrate formulation


P.S. Vimala Devi Laboratory Bt127 SC formulation Methodology:

Supply of SC formulation:

To AICRP centres of castor, sunflower, cotton, chickpea, groundnut etc. Experiment 2. Large scale field evaluation of Bt-127 SC formulation as a component of

IPM against major lepidopteran pests of sunflower


P. Duraimurugan Field Field efficacy of Bt-127 SC formulation

as a component of IPM in sunflower

81

Methodology:

Treatments: 2 IPM module and Farmer’s practice)

1. IPM module

Seed treatment (5g/kg seed) and foliar spray of Imidacloprid (0.2 ml/l) for

management of sucking pests

Monitoring Helicoverpa armigera and Spodoptera litura using pheromone trap

(4/acre)

Application of DOR Bt-127 SC formulation @ 3 ml/l for H. armigera, S. litura and

Thysanoplusia orichalcea when early instar larvae noticed

Use of reflecting ribbons for avoiding / reducing damage by birds

2. Farmer’s practice

Application of chemical insecticide as per FP

Details of Experiment

Village: Latur, Maharashtra

Design: Large field plot (1 acre each for IPM & FP)

Season: Kharif 2019

Date of sowing: IInd fortnight of June / Ist fortnight of July

Cultivar: Popular cultivar of the region

Observations

Number of head borers and defoliators larvae per plant

Per cent defoliation will be recorded in 50 randomly selected plants at fortnightly

intervals

Number of parasitoid stages and predators (spider, green lacewings & coccinellids)

per plant will be recorded in 50 randomly selected plants at fortnightly intervals

Seed yield and Economics

Experiment 3. Shelf-life data generation of Bt127SC formulation as per registration

guidelines


P. S. Vimala Devi Laboratory Shelf-life of Bt-127SC formulation Methodology:

• Formulation will be stored in HDPE bottles for 24 months at Akola centre of AICRP

(sunflower). One bottle will be sent by the centre to IIOR at 3-month intervals for

generation of data

• Data on shelf-life will be generated at IIOR for the 24th month sample on the

following parameters

Heat viable spore count

pH

Bacterial and fungal contaminants

Human pathogens

Efficacy against second instar larvae of S. litura and H. armigera Others:

• Generation of LC50 data of Bt-127 SC formulation against S. litura from ICAR-IIMR,

Hyderabad

82

Title Development of practicable technologies for field level

exploitation of endophytic Trichoderma for amelioration of

biotic and abiotic stresses in crops

Principal Investigator R.D. Prasad (Plant Pathology)

Co-investigator(s) P. Lakshmamma (Plant Physiology)

Md. A. Aziz Qureshi (Soil Science)

K.S.V. P. Chandrika (Agril. Chemicals)

Funding source ICAR Network-AMAAS

Total Amount

Sanctioned

-

Fund for the year Rs. 8.2 lakhs

Year of start 2017

Year of completion 2020

PIMS No. OXX00657

Objectives 1. To isolate and characterize potential strains of Trichoderma

(endophytic strains with broad host range, ameliorators of

biotic and abiotic stresses, enhancers of plant growth)

2. To develop biopolymer based formulations of

Trichoderma/consortia for efficient delivery

3. To evaluate biopolymer based products in different agro-

climatic zones, generate data required for product

registration with CIB & RC and commercialization of

potential technologies


Scientist(s) Activity

R.D. Prasad Evaluation of shelf life of biopolymer (chitosan) based

Trichoderma formulation in various agro-climatic conditions

(Locations-Coimbatore, Akola, Ludhiana and Hyderabad)

R.D. Prasad

K.S.V.P. Chandrika

Field evaluation of biopolymer (chitosan) based Trichoderma

harzainum,Th4d liquid formulation against seed and soilborne

diseases of Oilseed Crops

R.D. Prasad

K.S.V.P. Chandrika

On farm validation of biopolymer (chitosan) based Trichoderma

harzainum,Th4d liquid formulation against seed and soilborne

diseases of soybean and groundnut

Md. A. Aziz Qureshi

R. D. Prasad

Field evaluation of biopolymer based Trichoderma formulations

for amelioration of saline stress in natural saline soils (EC=6) in

sunflower

P. Lakshmamma

R. D. Prasad

Field evaluation of biopolymer based Trichoderma formulations

for amelioration of drought stress in castor

Activity 1: Evaluation of shelf life of biopolymer (chitosan) based Trichoderma formulation

in various agro-climatic conditions (Locations-Coimbatore, Akola, Ludhiana and Hyderabad)


R.D. Prasad Laboratory Stability of biopolymer based Trichoderma

formulation in different climatic conditions will be

known

Methodology: Formulation samples stored in 4 different agro-climatic conditions will be

obtained at monthly intervals and cfu will be enumerated by plating on selective medium.

83

Activity 2: Field evaluation of biopolymer (chitosan) based Trichoderma harzainum,Th4d

liquid formulation against seed and soil borne pathogens of Oilseed Crops


R.D. Prasad

K.S.V.P. Chandrika

Field Potential effects of biopolymer based

Trichoderma seed treatment known Methodology: Experimental field evaluation will be taken up as per the details on treatments, crops and

locations provided below. Treatments T1: Chitosan polymer+ T. harzianum Th4d @ 10ml/kg seed

T2: Polymer 1 + T. harzianum Th4d @ 10ml/kg seed

T3: Carboxin + thiram 2g/kg

T4: T. harzianum Th4d

T5: Control Crops and Experimental details Soybean Groundnut Safflower

Variety: JS-335

Season: kharif

Location: IIOR

Replications : 4

Plot size: 5 x 2.4M

Spacing: 30 x 10cm

Design: RBD

Variety:kadiri-6

Season: rabi

Location: IIOR

Replications : 4

Plot size: 5 x 2.4M

Spacing: 30 x 10cm

Design: RBD

Variety: PBNS-12

Season: rabi

Location: ICRISAT farm

Replications : 4

Plot size: 5 x 2.25M

Spacing: 45 x 20cm

Design: RBD Observations: Seed germination, disease incidence, seedling vigor, seed yield other yield components Activity 3: On farm validation of biopolymer (chitosan) based Trichoderma harzianum, Th4d

liquid formulation against seed and soil borne diseases of soybean and groundnut

Methodology Experimental field evaluation will be taken up as per the details on treatments, crops and locations

provided below. Treatments T1: Check

T2: Trichoderma + Chitosan polymer

T3: Trichoderma + Polymer 1

T4: Carboxin + thiram @ 2g/kg or tebuconazole 1g/kg Crops and experimental details Soybean Groundnut

Variety: JS-335; Season: kharif

Location: Adilabad

Plot size: 1 acre for each treatment

Spacing: 30 x 10 cm

Variety: kadiri-6; Season: rabi

Location: Nagarkurnool

Plot size: 1 acre for each treatment

Spacing: 30 x 10 cm


R. D. Prasad

K.S.V.P. Chandrika

Field Potential of biopolymer-Trichoderma

under field conditions known

84

Observations: Seed germination, disease incidence, seedling vigor, seed yield other yield components Activity 4: Field evaluation of biopolymer based Trichoderma formulations for amelioration

of saline stress in natural saline soils (EC=6) in sunflower


M. Aziz Qureshi

R.D. Prasad

Field Identification efficient polymer-Trichoderma

combinations that can support growth of

sunflower crop under salinity stress Methodology: Seed treated with and without polymers + Trichoderma combination as per

experimental details provided below will be sown in natural saline fields and observations on

seed germination and other growth parameters will be recorded. Treatments T

1: Untreated

T2: T. asperellum A5

T3: T. asperellum A5 + Polymer 1

T4: T. asperellum A5 + Chitosan

Season: late rabi

Location: ARS, Gangavati

Variety: DRSH-1

Replications: 5;

Spacing: 60 x 30cm

Plot size: 3 x 5M

Design: RBD

Observations: seed germination, seedling vigor, seed yield other yield components Activity 5. Field evaluation of biopolymer based Trichoderma formulations for amelioration of

drought stress in castor


P. Lakshmamma R.D. Prasad

Field Identification of efficient combination of polymer and Trichoderma for imparting drought tolerance in castor

Methodology

Field study: Seeds will be treated with potential combinations of polymer + Trichoderma and

sown in field during late rabi and drought stress will be imposed from 30-90 DAS or by

giving 100% irrigation to control and 40 % to stress for entire crop growth period Treatments

T1: Untreated

T2: Trichoderma + polymer 1

T3: Trichoderma + Chitosan polymer

Season: rabi

Location: Narkhoda farm

Cultivar: DCH-519

Plot size: 4.5 x 6M

Spacing: 90 x 60cm

Design: Split plot

Observations: Yield, yield components, DSI.

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Scientist(s) Activity

R.D. Prasad Quantification of castor gray mold disease severity under field

conditions and sending SMS alerts on gray mold occurrence

Ch. Sarada, R.D. Prasad Fine tuning of castor gray mold DSS and its validation Activity 1: Quantification of castor gray mold disease severity under field conditions at 4

locations (Poly house and Field, IIOR), Madhanpally (Makthal MO), Narva (Narwa MO) and

Undyal (CC Kunta MO) and collection of data on weather variables through wireless sensor

networks and disease severity values manually.


R.D. Prasad Field experiment

Favorable combinations of temperature,

humidity and wetness hours for disease

development known Details of experiment: A field experiment will be taken up as per treatment details

mentioned below

Treatments: Two (1 acre each of un-sprayed and fungicide sprayed)

Variety/hybrid: DCH 519; Season: Kharif 2019

Locations: 4 (IIOR, Hyderabad; Madhanpally (Makthal Mo) Narwa (Narwa Mo) and

Undyala (CC Kunta Mandal)

Observations: Half hourly interval record of temperature, humidity and leaf wetness in crop

canopy from 2 points in same filed will be collected in central server at IIOR

through WSN. Daily disease severity data also will be collected manually.

Title Proactive mitigation of gray mold (Botryotinia ricini) disease of castor (Ricinus communis L.) crop in Telangana State using dynamical disease forecast

Principal Investigator R.D. Prasad (Plant Pathology) Co-investigator(s) Ch. Sarada, (Agrl. Statistics) Funding source DST - SSTP Total Amount Sanctioned

-

Fund for the year Rs. 5.0 lakhs Year of start April 18, 2016 Year of completion December, 2019 PIMS No. IXX12628 Objectives 1. To establish relation between weather parameters and incidence

of gray mold disease of castor 2. To develop weather based disease prediction models of

castor gray mold 3. To develop a decision support system by integrating developed

disease prediction models 4. Deployment of Wireless Sensor Networks (WSN) in identified

farms and validation of DSS under field conditions (Research farm and hotspot areas )

5. Development of dynamic web and mobile based tools for online and real-time analysis, advisory dissemination and outreach to stakeholders

86

Activity 2: Fine tuning of castor gray mold DSS and Validation


Ch. Sarada, ACRC and C-DAC

Lab and Field Developed models will be validated with field data, refined and rebuild the model/ propose new model for forecasting the disease based on the new data sets collected from polyhouse, farmer’s fields. Fine tuning of DSS and Generation of alerts based on WSN data

Methodology: Interactive web interface created will be further refined and validated by

accessing each location data on temperature, relative humidity and leaf wetness collected by

each mote/ point of the locations where WSNs will be installed.

The data collected from WSNs will be formatted, curated and tabulated for statistical

analysis. Data will be fitted to linear and nonlinear regression models and Artificial Neural

Network models. Each experimental model will be validated based on residual properties for

considering the model. Selected models will be tested for goodness of fit statistics to select

the best model for forecasting. Selected forecast model will be validated based on the test

data and field data.

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Objectives

To discover genome-wide SNP markers in safflower based on the whole-genome

sequences of three genotypes, EC-523368-2, A-1 and CO-1

To design SNP genotyping assays for trait mapping purpose

To develop SNP based genetic linkage map of safflower using the recombinant inbred

line (RIL) population of CO-1 x EC-523368-2

To map genes/QTLs associated with resistance to aphid in safflower using the RIL

population of CO-1 x EC-523368-2

Experiments/Activities (objective wise) Objective 2: To develop SNP based genetic linkage map of safflower using the

recombinant inbred line (RIL) population of CO-1 x EC-523368-2


P. Kadirvel SNP/SSR genotyping of RILs SNP/SSR based linkage map for QTL

analysis Material

• Parents CO1 and EC-523368-2 and a set of 282 F6-RILs Methodology

• KASP assay based genotyping (50 SNPs)

• SSR based genotyping (25 SSRs) Observations

• SSR/SNP allelic segregation data Objective 4: To map genes/QTLs associated with resistance to aphid


P. Kadirvel Advancement of F9-RILs A set of 250 F10-RILs produced Material

• A set of 250 F9-RILs of the cross: CO-1 x EC523368-2 Methodology

• Selfing and Single Seed Descent

• Plot size : 1 row of 5 m length


• Season : Rabi


• Farm : ICARISAT


Title Mapping of QTLs associated with resistance to aphid (Uroleucon compositae Theobald) in safflower (Carthamus tinctorius L.) using genome‐wide SNP markers (DST funded)

Principal Investigator P. Kadirvel Co-investigator(s) P.S. Srinivas

S. Senthilvel Funding source SERB, DST Total Amount Sanctioned Rs. 38,24,064/-

Fund for the year Rs. 08,00,000/- Year of start 06-02-2017 Year of completion 05-02-2020 PIMS No. OXX03777

88


Objective 1: To demonstrate the productivity potential and profitability of improved

cultivars, production and protection technologies vis-a-visa farmers

practices under real farm situations


G. D. Satish Kumar

Conducting of FLDs with improved technologies of castor

Conduct of 125 FLDs on castor

Compilation of report on FLDs conducted by AICRP castor centres

Report on 1000 FLDs on castor

Pre-season trainings (two) to farmers on castor improved production technologies

Conduct of two trainings to farmers and one training to input dealers

Creating awareness on improved technologies of castor among farmers, staff of agricultural department, KVK scientists

Organizing two field days

Monitoring of FLDs conducted by PRDIS Conduct of 25 FLDs

Technology to be demonstrated: DCH-519/ ICH-66, spacing 90 cm X 60 cm, seed

treatment with Trichoderma, spray of Bt SC-127 formulation for control of

lepidopteron pests, gray mold management, Castor + red gram intercropping (5:1/1:1)

Plot size: 1 acre Design: Farmer fields

Season: kharif and rabi

Observations: Yield and economics data of improved technology and farmers

practice

Date of sowing: June 2nd fortnight to July last week

Districts, Mandals and Villages: Mahabubnagar- Narwa: Lankala & Yankee Makthal:

Gudigandla and Wanaparthy - Kothakota: Kanimetta Scientist(s) Activity-2 Deliverable(s) G. D. Satish Kumar, K. Alivelu

Conducting of FLDs on sunflower Conduct of 130 FLDs on sunflower Pre-season training of farmers Conduct of two trainings

Compilation of report on FLDs con-ducted by AICRP sunflower centres

Compilation of data of 900 FLDs conducted by AICRP centres

Creating awareness on improved technologies of sunflower among farmers, staff of agricultural department, KVK scientists

Conduct of one field day

Title Frontline Demonstrations (FLDs) on oilseeds and other extension activities

Principal Investigator G.D. Satish Kumar

Co-investigator(s) S.V. Ramana Rao C. Sarada

Funding source National Food Security Mission (NFSM) under DAC & FW Total Amount Sanctioned

Rs. 250 lakhs/year

Fund for the year Rs. 1.72 lakhs Year of start 2019 Year of completion 2020

PIMS No. - Objectives 1. To demonstrate the productivity potential and profitability of

improved cultivars, production and protection technologies vis-a-visa farmers practices under real farm situations

2. To impart training on improved oilseed production technologies to stakeholders (agricultural officers, extension workers and input dealers)

89

Technology to be demonstrated: DRSH-1, thinning and spray of B @ 2 ml/liter as directed

spray to capitulum. Application of elemental sulphur

Plot size: 1 acre Design: Farmer fields Season: rabi

Observations: Yield and economics data of improved technology and farmers practice

Date of sowing: November sowing

District: Hegdoli village, Nizamabad


S.V.Ramana

Rao

Conducting of FLDs on safflower Conduct of 50 FLDs on safflower

Compilation of report on FLDs

conducted by AICRP safflower

centres

Compilation of data of 500 FLDs

conducted by AICRP centres

Technology to be demonstrated: PBNS-12 and aphid management

Plot size: 1 acre Design: Farmer fields Season: rabi


Date of sowing: October

District: Vikarabad


C.Sarada and

G.D.Satish Kumar

Conducting of FLDs on

sesame

Conduct of 25 FLDs

Technology to be demonstrated: GT-10/Swetha Til/YLM-66/CUMS-17, line sowing,

Management of Macrophomina

Plot size: 1 acre Design: Farmer fields Season: rabi/summer


Date of sowing: January 2nd fortnight

District: Siddipet, Mahabubanagar and Ranga Reddy

Objective-2: To impart training on improved oilseed production technologies to

stakeholders (agricultural officers, extension workers and input dealers)


G.D.Satish

Kumar

Conducting of training programmes to

staff of KVKs, agricultural department,

input dealers and extension workers

Conduct of one training

programme

Monitoring of/participation in training

conducted by the AICRP centres

Ten trainings conducted on

oilseeds

90

Title Central Sector Scheme for Protection of Plant Varieties

and Farmers Rights Authority Principal Investigator N. Mukta Co-investigator(s) C. Lavanya

Mangesh Y. Dudhe Funding source PPV&FR Authority Total Amount Sanctioned - Fund for the year Rs. 10.25 lakhs Year of start 2008 Year of completion Continuous PIMS No. OXX01624 Objective(s) Conducting DUS trials for candidate varieties of sunflower,

castor and safflower, maintenance of reference collection and database


Activity-1 DUS testing of new candidate variety of castor (kharif, 2019)

Entries: One new candidate variety + 2 reference varieties

Plot size: 5 rows + 2 border rows x 6 m x 3 replications

Spacing: 90 x 60 cm

Date of sowing: July 2019 Observations: 30 DUS traits Activity-2 Characterization of farmers variety of castor (kharif, 2019)

Entries: Two farmers variety Plot size: 6 rows x 6 m Spacing: 90 x 60 cm

Date of sowing: July 2019 Observations: 30 DUS traits Activity-3 Seed multiplication of farmers variety of castor (kharif, 2019)

Entries: One farmers variety + One reference variety

Plot size: 6 rows x 6 m Spacing : 90 x 60 cm

Date of sowing: July 2019 Farm: Rajendranagar Area required for Activity 1, 2 & 3: 2000 m2 Activity -4 DUS testing of new candidate and VCK entries of safflower (rabi, 2019)

Entries: 2877/4134* & 2876/2327 + 6 reference varieties

Design: RBD No. of replications: 3 Spacing: 45 x 20 cm

Row length: 5 m No of rows: 8 Farm : Rajendranagar

Observations: 26 DUS traits

Area required: 800 m2

* One additional replication of new candidate for seed multiplication Activity-5 Characterization of sunflower reference varieties for additional traits (rabi, 2019)

Entries: 42 [13 hybrids, 8 varieties, 8 A-lines, 4 B-lines, 9 R-lines]

Plot size: 2 rows x 6 m x 2 replications Spacing : 60 x 30 cm

Farm : Rajendranagar Observations: 6 additional traits

Area required : 1000 m2

Activity-6 Maintenance and multiplication of reference varieties of castor and sunflower Castor (C. Lavanya)

Entries: 10 Parental lines/varieties

Plot size: 10 rows x 6 m Spacing : 90 x 60 cm

Season: Rabi 2019-20 Farm: Rajendranagar


Sunflower (Mangesh Y. Dudhe)

Entries: 20 Inbred parental lines

Plot size: 2 rows x 4.5 m Spacing : 60 x 30 cm

Season: Late Rabi 2019-20 Farm: Rajendranagar


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Seed production targets for 2019-20

Sl. No. Crop Variety/ Hybrid/

Parent

Target Acres Production

Target (q)

1 Castor DCH-519 10.0 60.0

2 M -574 2.0 5.0

3 ICS-164 1.0 3.0

4 DCH-177 10.0 50.0

7 ICH-66 80.0 500.0

8 DCS-107 2.0 10.0

9 Sunflower DRSH-1 10.0 30.0

11 CMS 243 A 0.5 1.0

12 CMS 243 B 0.5 1.0

14 DRSF-113 0.5 1.0

15 DRSF-108 0.5 1.0

16 Sesame GT-10 2.0 5.0

17 Shweta 5.0 12.0

18 YLM-66 2.0 5.0

19 CUMS-17 5.0 12.0

20 JCS-1020 2.0 5.0

21 Safflower DSH-185 2.0 2.0

22 ISF-764 20.0 150

23 ISF-1 1.0 3.0

Title Seed Production in Agricultural Crops

Principal Investigator

S.N. Sudhakara Babu

Co-investigator(s) T. Manjunatha H.P. Meena K.T. Ramya

Funding source ICAR Network Total Amount Sanctioned

-

Fund for the year Rs. 9.25 lakhs Year of start - Year of completion Continuous PIMS No. - Objective(s) 1. To organize Breeder Seed Production as per the DAC indent

of hybrids, varieties and parental lines of castor and sunflower 2. To organize seed production of popular varieties and hybrids

of castor, sunflower, sesame and safflower in farmers field under Revolving fund and Oilseeds Seed hub (certified seed) in participatory seed village mode.

3. Facilitating grow out test results with TSSCOA for seed certification and tags.

4. Facilitating/organizing sale and distribution of seed. 5. Organizing seed production of under TSP 6. Organizing trainings on seed production as per the mandate of

SPAC 7. Facilitating monitoring of breeder seed production of castor

and sunflower at centres 8. Compilation of activities of seed production

a compendium of technical programme (2019-20)

Documents