6/27/2011 1

NEW YORK MEDICAL COLLEGENEW YORK MEDICAL COLLEGEEnvironmental Health and Safety DepartmentEnvironmental Health and Safety Department

LABORATORY SAFETYLABORATORY SAFETYGood Laboratory PracticesGood Laboratory Practices

Training ProgramTraining Program

6/27/2011 2

ObjectivesObjectives

• Apply the recommended guidelines for Apply the recommended guidelines for personal laboratory safety personal laboratory safety

• Identify procedures when spills occursIdentify procedures when spills occurs

• Identify differences between different Identify differences between different categories of wastecategories of waste

• Identify and apply proper procedures for Identify and apply proper procedures for recycling recycling

6/27/2011 3

Rules and Regulations

• OSHA=Occupational Safety and Health OSHA=Occupational Safety and Health AdministrationAdministration

• EPA= Environmental Protection AgencyEPA= Environmental Protection Agency• NIH=National Institute of Health NIH=National Institute of Health • CDC= Center for Disease ControlCDC= Center for Disease Control• DEC= Department of Environmental DEC= Department of Environmental

ConservationConservation• NYSDOH= New York State Department of NYSDOH= New York State Department of

HealthHealth• DOT= Department of TransportationDOT= Department of Transportation• NYMC =Lab Safety ManualNYMC =Lab Safety Manual

6/27/2011 4

Proper AttireProper Attire

• When working in the lab you When working in the lab you MUSTMUST:: – Wear lab coatWear lab coat– Safety glasses (eye protection)Safety glasses (eye protection)– GlovesGloves

• When working in the lab When working in the lab NEVERNEVER wear: wear:– ShortsShorts– Open-toed shoesOpen-toed shoes– Tank-topsTank-tops

6/27/2011 5

At New York Medical CollegeAt New York Medical College

• ChildrenChildren and and PetsPets are not allow in are not allow in laboratories laboratories

• Security DeptSecurity Dept enforces this policy enforces this policy

6/27/2011 6

Use mechanical/electronic devices Use mechanical/electronic devices for pipettingfor pipetting

NEVER

6/27/2011 7

Be aware of:Be aware of:

• Dangling jewelryDangling jewelry

• Loose clothing andLoose clothing and

• Long hair Long hair

As they might get caught in equipmentAs they might get caught in equipment

6/27/2011 8

Use designated areas for eating Use designated areas for eating and drinkingand drinking

Do not wear lab coat in the cafeteria…!!!Do not wear lab coat in the cafeteria…!!!

6/27/2011 9

Do not wear lab coats, gloves or other personal Do not wear lab coats, gloves or other personal protective clothing out of the lab and into non-lab areasprotective clothing out of the lab and into non-lab areas

If gloves are necessary, have a glove in one hand only If gloves are necessary, have a glove in one hand only

6/27/2011 10

Wash your hands frequently throughout Wash your hands frequently throughout the day and before leaving the lab.the day and before leaving the lab.

6/27/2011 11

• Know where the eye wash and safety Know where the eye wash and safety shower is in your department areashower is in your department area

In case of splash in your eyes, In case of splash in your eyes, flush eyes with tap water for flush eyes with tap water for 15 minutes with eyewash15 minutes with eyewash

6/27/2011 12

Store foods and drinks only in Store foods and drinks only in designated refrigerators……designated refrigerators……

Food onlyFood only

Reagents onlyReagents only

6/27/2011 13

Cleaning and decontaminationCleaning and decontamination

• Clean your work area throughout the day and before Clean your work area throughout the day and before you leave at the end of the day.you leave at the end of the day.

• If necessary, clean equipment after use to avoid the If necessary, clean equipment after use to avoid the possibility of contaminating the next person who possibility of contaminating the next person who needs to use it.needs to use it.

• Keep all aisles and walkways in the lab clear to Keep all aisles and walkways in the lab clear to provide a safe walking surface and unobstructed provide a safe walking surface and unobstructed exit ways.exit ways.

6/27/2011 14

Non-hazardous wasteNon-hazardous waste

– Paper/Cardboard (recyclable)Paper/Cardboard (recyclable)– Food containers (cleaned can be recycled)Food containers (cleaned can be recycled)– Empty Pipette Tip boxes, Microcentrifuge tubes, Empty Pipette Tip boxes, Microcentrifuge tubes,

conical tubes, (recyclable)conical tubes, (recyclable)– Petri dishes used with non-infectious agents after Petri dishes used with non-infectious agents after

cleaned (can be recycled)cleaned (can be recycled)– Gloves Gloves not contaminatednot contaminated with hazardous agents go with hazardous agents go

in regular garbage non-recyclable)in regular garbage non-recyclable)– Empty chemical containers, glass/plastic, (deface Empty chemical containers, glass/plastic, (deface

label, rinse 3x with water=recyclable)label, rinse 3x with water=recyclable)

6/27/2011 15

Hazardous waste:Hazardous waste: BiologicalBiological

• BiologicalBiological: : DRYDRY waste that belongs in Red waste that belongs in Red BagsBags

• Human pathological wasteHuman pathological waste• Other Infectious wasteOther Infectious waste

• BiologicalBiological LIQUIDLIQUID Waste: Infectious Cultures Waste: Infectious Cultures and stocks must be treated overnight with a and stocks must be treated overnight with a freshly made 10%Bleach Solution and then freshly made 10%Bleach Solution and then discarded into the sink discarded into the sink (questions? Call EHS @ 594-4078)(questions? Call EHS @ 594-4078)

6/27/2011 16

Biological SpillBiological Spill

• In case of In case of biological biological hazard spillhazard spill use the use the emergency kit located emergency kit located inside the Chemical Spill inside the Chemical Spill Kit white bucket. Read Kit white bucket. Read carefully and follow carefully and follow instructions on orange instructions on orange label, then dispose inside label, then dispose inside a red bag containera red bag container

6/27/2011 17

Biological waste disposalBiological waste disposal

• Grey containers (32gal) in hallwaysGrey containers (32gal) in hallways

• Red containers lined with red bags in labsRed containers lined with red bags in labs

• Freezers in Animal Facility (dead animals)Freezers in Animal Facility (dead animals)

• Sharps containers: grey/red (17gal), Sharps containers: grey/red (17gal),

• Note: Dead animals : Dead animals SHOULD NOTSHOULD NOT be be kept in lab freezerskept in lab freezers

Chemical Inventory

• All hazardous chemical substances must be inventoried as they arrived to your lab

• The chemical inventory must be updated and every 6 months submitted to EHS

• Chemical substances must be arranged according to their physical/chemical characteristics

• Never arrange chemicals in alphabetical order in the shelves/cabinets. Its is a really bad practice

6/27/2011 18

6/27/2011 19

Hazardous waste:Hazardous waste: ChemicalChemical

• Chemical waste includes:Chemical waste includes:– Materials that posses hazardous Materials that posses hazardous

characteristics (e.g. toxic, flammable, characteristics (e.g. toxic, flammable, corrosive, reactive, combustible)corrosive, reactive, combustible)

– Old chemicals no longer usedOld chemicals no longer used– Chemicals past their expiration dateChemicals past their expiration date– Chemicals with illegible labels or without Chemicals with illegible labels or without

labels, or cracked containerslabels, or cracked containers– Any chemical by-product from completed Any chemical by-product from completed

experimentsexperiments

6/27/2011 20

Inherently Waste Like ChemicalsInherently Waste Like Chemicals

• *RCRA strictly regulates chemical use, which includes considering obsolete chemicals to be “Inherently Waste-Like.” A chemical may be considered Inherently Waste Like if:– There is an over-abundance of

a chemical in the laboratory.– The chemical container is

damaged– The chemical container or

label appear outdated– A label is damaged,

discolored, or unreadable.– A chemical has not been used

for 3 years*RCRA=resource conservation

recovery act

Pictured above are chemicals qualifying as “Inherently Waste Like”

6/27/2011 21

Small Chemical Spill (100ml or less)

• Accidental release / Accidental release / CHEMICAL SPILLCHEMICAL SPILL– Call Security @ 594-4226Call Security @ 594-4226– Ventilate area of leak or spillVentilate area of leak or spill– Remove sources of ignitionRemove sources of ignition– Wear PPEWear PPE

– Isolate hazard areaIsolate hazard area– Locate Chemical Spill KitLocate Chemical Spill Kit

– Contain and recover liquid when possibleContain and recover liquid when possible– Absorb with inert material and place in a chemical waste Absorb with inert material and place in a chemical waste

containercontainer

– Label the waste container (name of chemical and date)Label the waste container (name of chemical and date)– Place in Satellite Accumulation Area (SAA)–grey bin –Place in Satellite Accumulation Area (SAA)–grey bin –

6/27/2011 22

Chemical waste labeling/disposalChemical waste labeling/disposal

• LABELINGLABELING: containers that : containers that store hazardous waste store hazardous waste MUST BEMUST BE properly and clearly properly and clearly labeled:labeled:

Label should include:Label should include:• The words; The words;

””HAZARDOUS WASTEHAZARDOUS WASTE””• Chemical names of all Chemical names of all

ingredientsingredients

– Hazard associated: Hazard associated: Toxic, CorrosiveToxic, Corrosive

• DISPOSALDISPOSAL:: all chemical waste all chemical waste containers should be put in the containers should be put in the WASTE BIN or SAAWASTE BIN or SAA (satellite (satellite accumulation area) for pick up accumulation area) for pick up byTriumviratebyTriumvirate

Containers must be closed at all times when not adding waste. Do not mix incompatible wastes.

6/27/2011 23

Hazardous waste:Hazardous waste: RadioactiveRadioactive

• Radioactive waste includes: Radioactive waste includes: – Radioisotopes stock vials Radioisotopes stock vials – Any chemical by-product from completed Any chemical by-product from completed

experiments used with radioactive materials experiments used with radioactive materials (e.g. buffers, polyacrylamide/agarose gels )(e.g. buffers, polyacrylamide/agarose gels )

– Pipette tips, paper towel, transfer membranesPipette tips, paper towel, transfer membranes– Pickup of radioactive waste is done as often Pickup of radioactive waste is done as often

as needed and left for decay and final as needed and left for decay and final disposal in the Radioactive Materials Waste disposal in the Radioactive Materials Waste RoomRoom

6/27/2011 24

Hazardous waste:Hazardous waste: Pharmaceuticals Pharmaceuticals

• The Environmental Protection Agency–EPA-The Environmental Protection Agency–EPA-– Protect public health and the environment by Protect public health and the environment by

removing removing pharmaceuticalspharmaceuticals from waste from waste water treatment plants and municipal solid water treatment plants and municipal solid waste landfillswaste landfills

– Leave Pharmaceuticals in waste bins Leave Pharmaceuticals in waste bins in labs or Satellite Accumulation Area –SAA-.in labs or Satellite Accumulation Area –SAA-.

6/27/2011 25

Recyclable waste-1Recyclable waste-1

• SINGLE STREAM SINGLE STREAM RECYCLINGRECYCLINGThanks to new technology, Thanks to new technology, recyclable waste can be mixed at recyclable waste can be mixed at NYMC and put in NYMC and put in BLUE BINS BLUE BINS containerscontainers

• Acceptable items areAcceptable items are::cardboard, magazines, office paper, cardboard, magazines, office paper, junk mail, brown paper bags, junk mail, brown paper bags, paperboard, newspapers, plastic and paperboard, newspapers, plastic and glass bottles, aluminum cans and glass bottles, aluminum cans and foil, tin cans, empty aerosol cans.foil, tin cans, empty aerosol cans.

6/27/2011 26

Recyclable waste-2Recyclable waste-2

• Fluorescent tubes and Fluorescent tubes and compact fluorescents compact fluorescents lampslamps: because they may : because they may contain contain Mercury (Hg)Mercury (Hg)

• call EHS for info, x-4078call EHS for info, x-4078

BatteriesBatteries must be put in must be put in special containers through special containers through out the NYMC halls/lobbyout the NYMC halls/lobby

6/27/2011 27

Recyclable waste-3Recyclable waste-3

• toner toner MUST MUST BEBE sent back to sent back to manufacturer using manufacturer using free shipping labelsfree shipping labels

provided inside the provided inside the boxbox

6/27/2011 28

E-Waste @ NYMCE-Waste @ NYMC

• For disposal of For disposal of Electronic WasteElectronic Waste::– Monitors -Printers -Faxes machinesMonitors -Printers -Faxes machines– Computers -Keyboards -MouseComputers -Keyboards -Mouse

• Call the Office of Call the Office of Information-ServicesInformation-Services (IS)(IS) at at X-4582 toX-4582 to arrange a pickup arrange a pickup

6/27/2011 29

NON-Recyclable waste @ NYMCNON-Recyclable waste @ NYMC

• Food containers that are not cleanFood containers that are not clean• Food wasteFood waste• Styrofoam cupsStyrofoam cups• Styrofoam boxes (some boxes can be Styrofoam boxes (some boxes can be

returned to manufacturer with free returned to manufacturer with free shipping)shipping)

• Plastic bags (can be recyclable at local Plastic bags (can be recyclable at local supermarket)supermarket)

• Wood Wood

6/27/2011 30

Compressed Gas Cylinders-1Compressed Gas Cylinders-1

• All compressed gas cylinders must be All compressed gas cylinders must be properly stored at all timesproperly stored at all times

11

2

2

1

2

3

BOTH STRAPS

ARESECURED

BOTH STRAPS

ARESECURED

ALL THREE STRAPS

ARESECURED

6/27/2011 31

Compressed Gas Cylinders-2Compressed Gas Cylinders-2

• All compressed gas cylinders should be stored with capAll compressed gas cylinders should be stored with cap• Only one cylinder per bracket is allowedOnly one cylinder per bracket is allowed• Cylinder cannot be stored with a gaugeCylinder cannot be stored with a gauge• Cylinder should have a tag with name of investigator, room #, date, Cylinder should have a tag with name of investigator, room #, date,

indicate if is indicate if is FULL/ EMPTY/ IN USEFULL/ EMPTY/ IN USE

1 2

NO WALL

BRACKET

TWO CYLINDERSON ONE WALL BRACKET

ONE OF THESTRAPSIS NOT

SECURED

6/27/2011 32

Compressed Gas Cylinders-3Compressed Gas Cylinders-3

Violation of store codeViolation of store code IdentificationIdentification

6/27/2011 33

Recombinant DNA Recombinant DNA (rDNA)(rDNA)

• Before engaging in any research Before engaging in any research or academic activity, or academic activity, experiments involving rDNA, experiments involving rDNA, (even exempt experiments) must (even exempt experiments) must be registered, reviewed by the be registered, reviewed by the College College Biosafety OfficerBiosafety Officer (BSO)(BSO), and may have to be , and may have to be submitted for approval by the submitted for approval by the Institutional Biosafety Institutional Biosafety Committee (IBC)Committee (IBC)

• Call EHS for info, x-4078Call EHS for info, x-4078

6/27/2011 34

• Allergies: Facts– Allergies have been found to be associated

with most animals used in research labs– People develop allergic reaction to animal

protein found in urine, dander, and saliva– 70% of people with pre-existing allergies will

develop a new allergy– If you have allergies let your supervisor know

about it before your start working with animals

Comparative Medicine Dept.Comparative Medicine Dept.

6/27/2011 35

AutoclaveAutoclave GuidelinesGuidelines

• Training is a must for any person operating or handling an autoclave, it sterilizes by using High-Pressure Steam and High Temperature Conditions

• A practical training on Autoclave Safety from an experienced and knowledgeable person must be taken before using an autoclave

• Misuse can be very hazardous thus endangering human lives

• Always wear your personal protective –PPE- equipment when you are operating an autoclave

6/27/2011 36

Biosafety Cabinets (BSC)Biosafety Cabinets (BSC) GuidelinesGuidelines

• When working in the BSC When working in the BSC you must:you must:– Check the UV light is “off”Check the UV light is “off”

– Make sure the sash is at the Make sure the sash is at the correct sash heightcorrect sash height

– Avoid blocking the front and Avoid blocking the front and rear grills as they will decrease rear grills as they will decrease your personal protection as well your personal protection as well as cleanliness of your as cleanliness of your

experimentexperiment

6/27/2011 37

Chemical Fume HoodsChemical Fume Hoods GuidelinesGuidelines

• Before starting work in the hood, check that it is functional, that there is sufficient air flow, test with small piece of kimwipes ® or small amount of dry ice mixed with water

• Check for certification, that its current• Lower the sash to the proper operating

position• Work at least 4-6” inside hood• Airflow in a fume hood is easily

disrupted; do not use the fume hood for storage…!!!

• Hood not working? Report to Facilities @ 594-4588

6/27/2011 38

Cold RoomsCold Rooms GuidelinesGuidelines

• Rules are for Rules are for employee safety, to employee safety, to control control MOLDMOLD and and prevent contaminationprevent contamination

• No foodNo food• No beveragesNo beverages

• To prevent To prevent MoldMold growth, keep all growth, keep all benches cleanbenches clean

No storage inNo storage in• Cardboard Boxes Cardboard Boxes • Paper ProductsPaper Products

cell media

6/27/2011 39

DarkroomsDarkrooms GuidelinesGuidelines

• One of the most important rules in the darkroom is to One of the most important rules in the darkroom is to keep it keep it CLEANCLEAN and and FREEFREE of any paper waste and gels of any paper waste and gels because the drain gets clogged thus provoking floods because the drain gets clogged thus provoking floods that will affects other adjacent areas.that will affects other adjacent areas.

• Any waste generated by the user MUST be taken to the Any waste generated by the user MUST be taken to the lab and disposed of appropriately lab and disposed of appropriately

6/27/2011 40

SummarySummary

• In order to work safely in the lab you must In order to work safely in the lab you must follow proper safety proceduresfollow proper safety procedures

• Basic rules of hygiene govern a friendly Basic rules of hygiene govern a friendly safe and healthy environmentsafe and healthy environment

• Wastes generated in labs should be Wastes generated in labs should be carefully monitored for disposalcarefully monitored for disposal

• Recycling is one of your responsibilities Recycling is one of your responsibilities as an employee of New York Medical as an employee of New York Medical CollegeCollege

Done! Congratulations and now take a test by clicking on the link and follow the instructions: http://www.proprofs.com/quiz-school/story.php?title=laboratory-safety_7

6/27/2011 41