PATENT ABSTRACTS 179

5206150

C O M P O S I T I O N O F , M E T H O D O F P R O D U C I N G A N D M E T H O D O F

U S I N G A S T A B I L I Z E D F O R M U L A T I O N F O R A S S A Y I N G

P E R O X I D A S E A C T I V I T Y

Hsin-Hsiung Tai assigned to University of Ken- tucky Research Foundation

A formulation for use in detecting and/or deter- mimng peroxidase activity comprises a mixture in solution of tetramethylbenzidine, hydrogen peroxide, a buffering agent and bacitracin as a stabilizing agent. The formulation is used as a peroxidase substrate that is stable in solution for an extended period of time and provides en- hanced color sensitivity.

5206169

A N I M A L C A R C A S S C O M P O S T C R I B

Robert O Bland

A compost crib is provided for the on-site dis- posal of animal carcasses accumulated during commercial operations, in which pass~ive solar energy is utilized to assist in the decomposition process. Aerobic, thermophilic bacteria multiply in the sunlight, solar heat and heat of decomposi- tion and convert nitrogenous material in carcas- ses and manure, as well as the carboniferous ceUulose additive to the compost, to a bacterial biomass. The crib is simple in construction, having a frame with mesh walls to allow for ade- quate exposure to air. Access doors are provided for loading carcasses and for unloading recycled compost material for rotation back onto the compost mass. Solar panels are provided in the roof for the generation of solar heat and to pro- vide sunlight to aid in bacterial photosynthesis.

5206151

R A P I D S E L E C T I O N O F B I O C I D E U S I N G A R E D U C T I O N

O X I D A T I O N I N D I C A T O R S Y S T E M

Linda Robertson assigned to Naico Chemical Company

A rapid method for determining the minimum inhibitory concentration of biocides or biocidal agents for use in various microbiological con- taminated aqueous systems is disclosed. This rapid technique provides for an opportunity to determine the minimum amount of anti- microbial agents, or biocides either singularly or combination, to control microbiological growth in aqueous s t reams contaminated by micro- organisms. The test involves the use of reduction oxidation indicator dye systems which react to enhanced microbiological activity producing reducing enzymes such as dehydrogenase en- zymes. The technique provides an answer within a time period of from about 30 minutes to about 8-10 hours as opposed to normal testing pro- cedure that can require up to I-2 weeks. The test procedures uses multiple columns of multiple sample wells on a microtitration plate and tech- niques for transferring prescribed aliquots of contaminated aqueous systems, nutrients, redox indicator dyes, and incremental and serially diluted concentrations of anti-microbial agents.

5206170

S U L F U R D I O X I D E D E T E C T O R D E V I C E E M B O D I E D B Y , U S I N G

S 0 2 - O X I D A N T B I O A G E N T

Takeshi Sato, Nobuko Kubo, Hirofumi Akano, Yoshiya Kawamura, Shigesada Iijima, Toki, Japan assigned to Nakano Vinegar Co Ltd

In a sulfur dioxide detector using SO2-oxidant microbes, a bio-membrane is located at one end of an oxygen concentration sensor, the bio- membrane containing the SO2-oxidant microbes between an immobilized membrane and a gas permeable membrane. The sensor has an elec- trode in contact with the immobilized mem- brane. A dish-shaped or frusto-conical cell has an open end which is in contact with the gas per- meable membrane so that SO2-1aden solution supplied to the cell causes sulfurous acid to ox- idize to sulfuric acid as the SO2 permeates through the bio-membrane so as to change an output from the sensor. An inlet and outlet hole are formed on a sidewall of the cell. The outlet hole is located to be always directly above the in- let hole so as to drain foam formed when the SO2-1aden solution is supplied to the cell through the inlet hole and drained from the out- let hole.