130 PATENT ABSTRACTS

whereby the assay is conducted in a vial ha\- ing a volume of not more than 20 milliliters. preferably 7 ml. or less. by incubating sub- strate in buffer and sample, stopping the reaction after a predetermined time with. e.g., acid or base. adding the water-immiscible organic solvent containing scintillant, cap- ping the vial. mixing by inverting the vial whereupon a spontaneous phase separation occurs. The radioactivity of the organic phase is then counted in an appropriate scintillation counter and the enrymic activ- ity is thereupon calculated.

4372883

PROCESS FOR THE PRODUCTION OF VACCINE

Tyoku Matuhashi. Mitsuko Usui. Akio Yamamoto, MasakaTu Mitsuhashi. Shun-

saku Koyama, Kawaguchi. Japan. assigned to Kabushiki Kaisha Hayashibara Seibutsu

Kagaku Kenkyujo

The present invention relates to a process

for the production of vaccine. characterized by covalent attachment of a biologtcally toxic substance to a saccharide both to form a biologically toxic substance-saccharide conjugate and to detoxify said substance. followed by collection of the formed conju- gate. According to the invention, various vaccine products having a high productivity of immunoglobulin G and M antibodies. but without productivity of immunoglobulin E antibody, arc easily obtainable. In addi- tion, the vaccine products. upon administra- tion to animals, do not effect any allergic reaction and’or anaphylactic-shock due to the lack of the immunoglobulin E antibody

production.

4371622

MICROMONOSPORA CULTURE

Miklos Jarai, Sandor Piukovich. Sandor Istvan, Istvan Gado. Valeria S7ell. lstvan

Barta. Budapest, Hungary. assigned to CHINOIN GyogysTer es Vegyeszeti Ter- mekek Gyara R 7.

Micromonospora Danubiensis, a new spe- cies, having the capability of producing sisomicin.

4371611

ENZYMATIC DIAGNOSTIC COMPOSITION

Murrary C. Fusee. assigned to W.R. Grace & co

Disclosed herein is a method for bindtng enzymes comprising reacting either a syn- thetic or a natural polymer containing car- boxy1 and amino groups with an excess of a urethan prepolymer followed by coupling of an enlyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6 phosphate dehydrogenase to determine creatine phosphokinase (CPK) tn human blood.

4371466

MAMMALIAN COLLAGENASE INHIBITORS

William H. McGregor, assigned to Ameri- can Home Products Corporation

The polypeptides PS where X is hydrogen, alkanoyl of 2 to 6 carbon atoms. cycloalkyl- carbonyl of 6 to 8 carbon atoms or alkoxy- carbonyl of 2 to 6 carbon atoms: RI is