3rd chapter

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Chapter 3

MATERIALS AND METHODS

The present study was carried out in the research fied and !reen house of the Depart"ent

of #ant $reedin! and %enetics& 'ni(ersity of A!ricuture )aisaa*ad+ Thirty "ai,e

in*red ines were used in the two e-peri"ents+ The in*red ines used in the e-peri"ents

were ta.en fro" the Mai,e Research %roup& Depart"ent of #ant $reedin! and %enetics&

'ni(ersity of A!ricuture )aisaa*ad+ The e-peri"ent I was conducted in the !asshouse

and e-peri"ent II was carried out in the fied durin! /harif 0122+ )aisaa*ad is situated

32N and 42E+ The a(era!e "onthy "a-i"u" and "ini"u" te"perature and tota

"onthy rain fa of croppin! season is !i(en in fi!ure 2 and fi!ure 0 respecti(ey+

Ta*e 3+2+ In*red ines used in study+

Sr+ No+ In*red ine Sr+ no+ In*red ine

2 M25 26 OH752

0 A8170 24 OH78573A

3 A039 2: ;7657S#

5 A50470 29 ;7657TMS

8 A598 01 ;M23RA

6 A8179 02 ;)794 A80272 00 ;)TMS

: A858 03 ;2:4R

9 A886 05 ;21

21 A63: 08 ;A345:

22 AES015 06 ;:073

20 Anti!ua2 04 /88TMS

23 OH7: 0: %#)79

25 OH70: 09 'SSR51

28 OH73372 31 'SSR281

E-peri"ent 2

This e-peri"ent was conducted in the !reen house of the Depart"ent of #ant $reedin!

and %enetics& 'ni(ersity of A!ricuture )aisaa*ad+ The e-peri"ent is aid out in

co"petey rando"i,ed desi!n with four repications+ The factors are nor"a irri!ation

and water stress+ The in*red ines were sown in poythene *a!s fied with sand+ There



were 8 pants in each entry+ In pre7irri!ated sand the seeds were sown one inch deep with

hand+ Two nor"a repications were irri!ated first at si-th day of sowin! then twefths

day of sowin! and ei!hteenth day of sowin! whie other two drou!ht repications were

irri!ated at twefth day of sowin!+

After twenty four days of sowin! seedin!s were uprooted and washed+ It was

preceeded *y hea(y irri!ation to faciitate the process of uprootin! to "ini"i,e root

da"a!e+ After this pants were ta.en to a*oratory for the purpose of data coection+

Root en!th <c"=>

The root and shoot are separated *y a scaper at the node ?oinin! root and shoot+

The en!th of roots was "easured in c" *y usin! "eter rod+ A(era!e of fi(e pants per

!enotype per repication was cacuated+

Shoot en!th <c"=>

Shoot en!th was aso "easured in c" *y usin! "eter rod indi(iduay and

a(era!e was cacuated+

)resh root wei!ht <!=>

)resh root wei!ht of seedin!s were wei!hed in !ra"s *y usin! eectrica

wei!ht *aance < OHA'S7%T511&'SA=+ A(era!e of each in*red ine was cacuated in

one repication+

)resh shoot wei!ht <!=>

)resh shoot wei!ht of seedin!s were wei!hed in !ra"s *y usin! eectrica

wei!ht *aance < OHA'S7%T511&'SA=+ A(era!e of each in*red ine was cacuated in

one repication+

Dry root wei!ht <!=>

The sa"pes were dried in o(en <LEEC7)0& '/= at 68 C after ta.in! the

fresh root wei!ht up to co"pete dryin!+ Then the dry roots were wei!hed in !ra"s *y

usin! eectrica wei!ht *aance < OHA'S7%T511&'SA=+ A(era!e per repication was

cacuated+

Dry shoot wei!ht <!=>



The sa"pes were dried in o(en <LEEC7)0& '/= at 68 C after ta.in! the

fresh shoot wei!ht up to co"pete dryin!+ Then the dry shoots were wei!hed in !ra"s *y

usin! eectrica wei!ht *aance < OHA'S7%T511&'SA=+ A(era!e per repication was

cacuated+

Root@shoot ratio>

Root@shoot ratio for each entry was cacuated *y di(idin! the dry root

wei!ht *y dry shoot wei!ht+

Root@shoot ratio dry root wei!ht <!=@ dry shoot wei!ht <!=

Root density>

Roots were ta.en after wei!hed and dipped in water in a *ea.er to "easureroot density+

E-peri"ent II>

This e-peri"ent was conducted in the fied of the Depart"ent of #ant

$reedin! and %enetics& 'ni(ersity of A!ricuture )aisaa*ad+ The e-peri"ent is aid out

in rando"i,ed co"pete *oc. desi!n with four repications+ The factors are nor"a

irri!ation and water stress+ )i(e "eter on! one row of each !enotype is !rown in each

repication+ Seeds were sown on rid!es *y di**er .eepin! pant to pant 08c" distance

and row to row distance 48 c"+ A the a!rono"ic practices were .ept unifor" e-cept

irri!ation in a repications+

Three irri!ations were !i(en to drou!ht *oc.& whereas se(en irri!ations were !i(en to

the nor"a *oc.+ )i(e !uarded pants were seected rando"y per entry per repication

and data were recorded on the foowin! traits+

#ant hei!ht <c"=>

A*o(e the !round up to tip hei!ht is "easured in c" with "eter rod of seected

fi(e pants of each entry per repication+ A(era!e is cacuated+

Ste" dia"eter <c"=>

Ste" dia"eter of seected pants is "easured with inch tape in c"+ A(era!e is

cacuated+



No+ of ea(es per pant>

Nu"*ers of ea(es were counted on seected pants per entry per repication+A(era!e per repication is counted+

Days ta.en to tassein! and si.in!>

Nu"*er of days ta.en to tassein! and si.in! was counted fro" the date of

sowin! to the ti"e when "ore than 81 percent pants co"peted tassein! and si.in!+

Leaf an!e>

Leaf an!e of fa! eaf is "easured with D in de!rees+

%rain yied per pant>

Co*s were coected fro" seected pant& dried the"& desheed and wei!hed *y

eectrica wei!ht *aance <OHA'S7%T511&'SA=+ A(era!es were cacuated+

Statistica Anaysis>

Anaysis of (arianceAnaysis of (ariances was co"puted to co"pare the !enotypes for each trait in

e-peri"ent+ < Stee and Torrie& 29:5& Stee et a+& 2994=+ The indi(idua co"parisons of

"eans were acco"pished *y usin! DuncanBs New Mutipe Ran!e Test <DMR7test=+

Standard error& phenotypic and !enotypic coefficients of (aria*iity for each

character were aso cacuated+

Correation AnaysisCorreation anaysis was perfor"ed to esti"ate !enotypic and phenotypic correations

foowin! the statistica techniue outined *y /own ana Torrie <2965=+

3rd chapter

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