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3D ModellingIntegrated Life Cycle Sustainability Assessment
Diacids production from 2nd generation bio-refinery
Martina Prox, Michael Bruns, Martin Ramacher, Marten Stock, Mieke Klein,
Agenda
Meaning of BioREFINE - 2G
Partners and Work Packages
Life Cycle Sustainability Assessment
Results of LCSA
Conclusion
BIOREFINE - 2G approach
Utilisation of otherwise unused C5-wood sugars to produce diacids (throughengineered strains) as starting components for value-added biopolymers
ULUND & DTU – fermentation strain
Identifiying a strain and a fermentation protocol toenable:
• Up take of C5 sugars
• high production of diacid
Optimizing genetic and regulatory processes
within cells to increase the cells' production of a certain substance
ifu - Life Cycle Sustainability Assessment
Sustainability:
“Meeting current needs without
compromising the needs of future
generations”
Assessing the impacts of newly
developed process to ensure that
sustainability goals are met.
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Sustainability
Goal of Life Cycle Sustainability Assessment
• fully understand environmental, economic and social impacts of products produced by the newly developed bioprocess
• report on the sustainability of the products to the funding association
• serves as a showcase on the newly integrated methods regarding economic and social indicators and impacts into the framework of an already established Environmental Life Cycle Assessment
• support the dialogue with the project partners on the method of a LCSA and to inform on preliminary results based on the most recent developments
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Sustainability results
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Product Source Feedstock GWP [kg
CO2-eq/kg]
NREU
[MJ/kg]
Land use
[m2/kg]
Bio SA [23] Corn 0.3-1.3 28.0-66.5 1.5-2.6
Bio SA [23] Sugar cane -0.4-2.1 9.1-44.9 1.5-2.6
Bio SA [23] Corn stover -0.2-2.5 15.0-54.5 0.8-1.7
Pchem SA [24] MAn 1.95 59.2
Conv SA [22] MAn 98.2
SA 5 [22] Wood 148
Patent SA [25] Sugar beet 1.96 28.5 1.0
bioREFINE Wood sugars 8.2-12.9 162-211 15.5-28.7
[22] Morales, M., Ataman, M., Badr, S., Linster, S. et al., Sustainability assessment of succinic
acid production technologies from biomass using metabolic engineering. Energy Environ. Sci.
2016, 9, 2794–2805, DOI: 10.1039/C6EE00634E.
[23] European Commission, Environmental Factsheet: Succinic Acid.
[24] Cok, B., Tsiropoulos, I., Roes, A. L., Patel, M. K., Succinic acid production derived from
carbohydrates: An energy and greenhouse gas assessment of a platform chemical toward a bio-
based economy. Biofuels, Bioprod. Bioref. 2014, 8, 16–29, DOI: 10.1002/bbb.1427.
[25] Datta, R., Glassner, D. A., Jain, M. K., Roy, J., Fermentation and purification process for
succinic acid, Google Patents, 1992. http://www.google.com/patents/US5168055.
Social Life Cycle Assessment
• Based on UNEP/SETAC
Guidelines
• Qualitative only
• Questionnaire forsite-specific assessment
• Literature research for specific
region/sector assessment
• Social Hotspot Database to assess supply chain
• Site performance good with very
low to low social risks
• Good performance for background
processes in norway
• Wood and chemicals supply should
be assessed specific when theprocess gets implemented
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Conclusions
• LCSA: integrated modelling should be performed to enableassessments of processes in early design phase
• Preliminary results identified hotspots for ongoing processdesign improvements.
• Uptake of C5-sugars and production of diacid pose double challenge. Additionally complex wood sugar medium includingcontaminants and inhibitorsand requires improvements toenable sustainability goals
• Auxiliaries to condition waste streams need to be reduced. Production of diacid not yet implemented on scale-up. Assessment should be updated with ongoing improvements.
Thank you very much for your attention!
Do you have any questions?
Martina Prox
[email protected] biorefine2g.de
DTU – microbial engineering
Metabolic engineering of strains for production of wide variety of chemicals needs several rounds of genetic interventions
• stress resistance
• robustness
• high fermentation capacity
WIP – Dissemination and Exploitation
• Marketing material, homepage, cooperations,
and bio-conferences
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