25 years of excellence in biopesticide development - accueil · dr. o. parisi prof. m.h. jijakli 25...
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Dr. O. Parisi
Prof. M.H. Jijakli
25 years of excellence in
Biopesticide development
to serve you
Screening Strategies
Biopesticides R&D
Screening Strategies
Bio-control with the
IUPPL*
Natural extracts or
molecules, enzymatic
systems …
Essential oils
Elicitors
Microorganisms
IUPPL: Integrated and Urban Plant Pathology
Laboratory
Natural extracts /
EOS; enzymatic
systems … Rapid Methodology
• 100 products or molecules/
week
•8 replicates
•From 4 to 2 pathogens/week
•Measure of OD /24 h
•120h
•Measure of OD /2 h
•24h
Rapid screening technology
on ELISA Plate for pathogens
growing in liquid medium
•3 Plates / object
•MAX 50 objects/ assay
•From 2 to 4 pathogens /
week
•Results in 5 to 7 days
Screening on Petri plates
for pathogens restricted
to solid medium
Poisoned food technique
(product incorporated into the
medium)
Paper filter technique (5mm
filter soaked with the product)
Elicitors
Microorganisms
Screening on
plants
Screening on plants:
•MAX 5 objects / assay on
preharvest models
•MAX 10 objects / assay on
postharvest models
•Results in 5 days to some weeks
depending on the model
Direct In vivo screening for
microorganisms and elicitors:
no correlation between in
vitro AND in vivo behavior
Efficient products
Need of In Vivo Evaluation
Phytotoxicity??
•In greenhouse or culture chamber:
•On plants including fruits and
seeds
•Preferably on young leaves
•Max 5 objects / assay in
Preharvest Models
•Max 10 objects / assay in
Postharvest Models
Products (Active Ingredient and/or
formulation) qualified for in vivo studies need
to be characterized for their phytotoxicity.
If needed formulation adaptation can be
envisaged to reduce the eventual
phytotoxicity
Efficient, not phytotoxic,
adequate sourcing
Serious candidate
In vivo evaluation
•Preceded by a symptoms reproduction
•Max 5 objects / assay in Preharvest
Models
•Max 10 objects / assay in Postharvest
Models
Efficient, non phytotoxic products available
in adequate quantity or disposing of an
adequate sourcing can be evaluated in
controlled (in greenhouse or in culture
chamber) or in semi-practical conditions (in
experimental fields)
Two independent assays
to validate the results English or French report
Adapted statistical
analyses of the data Raw data and analyzed
dat provided on
demand
Accurate Scientific
advices
IUPPL
Services and
Forces
Confidential
management
25years of expertise
Fu
ng
icid
es
- B
ac
teri
cid
es
Crops Pathogen Conditions of
realization
Trial duration
Citrus fruit
(oranges)
Fruits Penicillium
digitatum
(green mold)
Conditioning
chamber
4 weeks (at 25°C)
Penicillium italicum
(blue mold)
Conditioning
chamber
4 weeks (at 25°C)
Banana Piece of leaves in
survival
Mycosphaerella
fijiensis
(Black sigatoga)
Petri plates 14 weeks (at 28°C)
Harvested fruits Colletotrichum
musea
(one of the agents
of the crown rot)
Conditioning
chamber
6 weeks (at 25°C)
Beet Plant Cercospora beticola greenhouse 4 weeks (ambient T°)
Plant Erysiphae betae
(powdery mildew)
greenhouse 4 weeks (ambient T°)
Plant Rhizoctonia solani greenhouse 4 weeks (ambient T°)
Cucumber seeds Pythium ultimum
(damping-off)
greenhouse 8 weeks (ambient T°)
Plants Sphaerotheca
fuliginea (Powdery
mildew)
greenhouse 4 weeks (ambient T°)
flowers Cut flowers Botrytis cinerea
(grey mold)
Conditioning
chamber
4 weeks (at 25°C)
strawberry Dried leaflet Botrytis cinerea
(grey mold)
Petri plates 8 weeks (à 25°C)
Harvested fruits Botrytis cinerea
(grey mold)
Conditioning
chamber
4 weeks (at 25°C)
Plants Xanthomonas
fragaria
Quarantine Lab
(L2Q)
8 weeks (at 25°C)
Plants Podosphaera
aphanis (oïdium)
Greenhouse 4 weeks (ambient T°)
List of efficacy trials available at the Plant Pathology Laboratory following the crops and the pathogens In vitro assays can be performed on each pathogen excepted for the biotrophic pathogens (like powdery mildew, the stripe rust, and V. inaequalis)
Fu
ng
icid
es
- B
acte
ric
ide
s
Crops Pathogen Conditions of
realization Trial duration
wheat Grains Penicillium verrucosum Glass mini-silo 6 weeks (at 25°C)
Plants Septoria tritici Greenhouse 4 weeks (ambient T°)
Fusarium graminearum / F culmorum
Greenhouse 4 weeks (ambient T°)
Bean Semences Pythium ultimum (damping-off)
Greenhouse 8 weeks (ambient T°)
Rhizoctonia solani (fonte des semis)
Greenhouse 8 weeks (ambient T°)
Plants Colletotrichum lindemuthianum
Greenhouse 12 weeks (ambient T°)
Kiwi Harvested fruits Botrytis cinerea (grey mold)
Conditioning Chamber
4 weeks (at 25°C)
Onion Bulbs Botrytis aclada (Neck rot)
Conditioning Chamber
4 weeks (at 25°C)
Pear Fruits Botrytis cinerea (grey mold)
Conditioning Chamber
4 weeks (at 25°C)
Penicillium expansum (blue mold)
Conditioning Chamber
4 weeks (at 25°C)
Apple Fruits Botrytis cinerea (grey mold)
Conditioning Chamber
4 weeks (at 25°C)
Penicillium expansum (blue mold)
Conditioning Chamber
4 weeks (at 25°C)
Gloesporium sp. Conditioning Chamber
4 weeks (at 25°C)
Rhizopus stolonifer Conditioning Chamber
4 weeks (at 25°C)
Mucor sp. Conditioning Chamber
4 weeks (at 25°C)
Fusarium sp. Conditioning Chamber
4 weeks (at 25°C)
Monilia sp. Conditioning Chamber
4 weeks (at 25°C)
Alternaria sp. Conditioning Chamber
4 weeks (at 25°C)
Pieces of leaves Venturia inaequalis (apple scab)
Petri plates 16 weeks (25°C , then Cold T°)
In vitro Erwinia amylovora Petri plates 2 weeks
In vitro Pseudomonas syringae Petri plates 2 weeks
List of efficacy trials available at the Plant Pathology Laboratory following the crops and the pathogens In vitro assays can be performed on each pathogen excepted for the biotrophic pathogens (like powdery mildew, the stripe rust, and V. inaequalis)
Fu
ng
icid
es
– B
ac
teri
cid
es
Crops Pathogen Conditions of
realization
Trial duration
Potato Plants Phytophthora
infestans
(downy mildew)
Greenhouse 9 weeks (ambient T°)
Tubers (and plants) Pectobacterium
carotovorum
Conditioning
Chamber
3 weeks (25°C)
Plants Rhizoctonia solani Greenhouse 12 to 16 weeks
Tomato Dried leaflets Botrytis cinerea
(grey mold)
Petri plates 8 weeks (at 25°C)
Tulip Bulbs Botrytis tulipae
(tulip fire)
Conditioning
Chamber
4 weeks (at 25°C)
Vine Plants Uncinula necator
(powdery
Greenhouse 6 weeks (ambient T°)
Soil disinfection Sowed soil Pythium ultimum Greenhouse 4 weeks (ambient T°)
In vitro Verticillium dalhia Petri plate 2 weeks (at 25°C)
Herb
icid
e
water hyacinth Greenhouse Ambient T°
List of efficacy trials available at the Plant Pathology Laboratory following the crops and the pathogens In vitro assays can be performed on each pathogen excepted for the biotrophic pathogens (like powdery mildew, the stripe rust, and V. inaequalis)
