21 .11. 2556 - ubon ratchathani university
TRANSCRIPT
21 lMfl1fIJ1J 11 2556
Development of electronic nose system to classify garlic type
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(o1tt1elmqtt) Development of electronic nose system to classify garlic
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system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
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LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~
III I r1 ltI 1
lilT 11 11 l~
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_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1
UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v
d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
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tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
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eV
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17
18
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20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
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= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
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Sensor Array
oXoXoXoXO
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conditioning -tilgt
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Computer
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
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LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
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42 fl1iVlVl6itll1Vln~UmLVialJ
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
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15
15
15
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15
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5
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200
400
600
800
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60
0 80
0 0
0
200
400
600
800
T
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(sec
) T
ime
(sec
) T
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(sec
) T
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(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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200
400
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200
400
600
800
T
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(sec
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(seo
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sec)
T
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No
9 N
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N
o12
2
22
shy
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1
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0
200
400
800
600
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0 80
0 o
200
400
800
800
0 20
0 40
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0
Tim
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Tim
e (s
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0 0
200
400
600
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0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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00
o
200
400
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0 20
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200
400
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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T
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(sec
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No
9
No
IO
No
1
No
12
2
2
2 2
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15
15
15
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200
400
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20
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0 0
200
400
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T
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(sec
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bullbullbullbullbull
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20
N
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1
No2
N
o3
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4
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5
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N
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3 N
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15
15
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5
No
6 N
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8
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r TI
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(sec
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(sec
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(sec
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TIm
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ec)
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MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
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S261
1
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620
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800
00
200
400
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400
silo
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00
200
400
600
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atl
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
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12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
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n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU
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bull bull
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UVIAflUB i
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UVIaltl~U~II1l iii d 0
UVIVI 1 UVIU1 1
11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1
ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1
13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI
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i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ
UVIVI 2 VIqlef~VILnll1t1i)4 4
21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5
23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G
24 n1iai1JGl1l1VlJVf1LBULB6
251t1iLLnilJ LabVIEW ~ 8
26 Principal Component Analysis (PCA) 8 ~1t1 ~ II
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31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d
32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0
322 oJGln1lVIflGlBJ 13
323 1~1iwt-JGln1lVIflGltlJ 14
33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15
332 t-JGln1aVIflGlBJ 15
323 11iW~Gln1lVIflGlBJ 19 41 tI CII
UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0
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422 ~Gln1iVWIGleJ~ 25
43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29
J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31
LeJn~1ieuroh~E~ 33
n91~n1It1in1f1 35
n1f1~1J1n n 36
d UVIVI 1
o UVIU1
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~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111
JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII
lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ
I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl
~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II
~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l
1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or
Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1
- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I
12 1ltl5i1~Alla~lf1middotHn151~1J
1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U
d II 0 tf
2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ
3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll
13 lIaULlIlIa~lflJ~n1J1~1J
1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ
ElLflnVliElUn~
2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1
2
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43 LtRmJLVitluiiillJfInnifil
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15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl
- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose
system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4
tJLGlnVI~tJUnG1
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LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~
III I r1 ltI 1
lilT 11 11 l~
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fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V
_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1
UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v
d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
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m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
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nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
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nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
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3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
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4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
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tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
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CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
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mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
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Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
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LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
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BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
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l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
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41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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23
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(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
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---
No1
No
2 N
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4
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15
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400
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200
400
600
800
T
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(sec
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(sec
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(sec
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5 N
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400
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sec)
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Tim
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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~
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32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
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4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
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5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
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7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
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9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
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Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
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_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
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d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
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17
18
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20
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
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= ~1E1rluqUU l
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1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
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Sensor Array
oXoXoXoXO
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
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LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
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15
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5
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200
400
600
800
0 0 20
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60
0 80
0 0
0
200
400
600
800
T
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(sec
) T
ime
(sec
) T
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(sec
) T
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(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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200
400
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o 20
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0 o
200
400
600
800
T
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(sec
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(seo
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ne (
sec)
T
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No
9 N
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o1I
N
o12
2
22
shy
2
1
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0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
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Tim
e (s
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0 0
200
400
600
800
0 20
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0 0
200
400
600
800
Ti
me
(sec
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me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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00
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200
400
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0 20
0 40
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200
400
600
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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No
9
No
IO
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1
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12
2
2
2 2
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15
15
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05
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200
400
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20
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0 0
200
400
600
800
T
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(sec
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bullbullbullbullbull
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26
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20
N
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1
No2
N
o3
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4
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5
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N
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3 N
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15
15
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0
0
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5
No
6 N
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r TI
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(sec
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(sec
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(sec
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TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
o12
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S261
1
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2 2
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620
~ ~
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u
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00
200 ~ ~
800
00
200
400
800 ~
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400
silo
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00
200
400
600
800
TIm
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atl
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
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6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
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8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
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9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
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11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
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12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
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14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU
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II do ~
1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ
mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1
bull bull
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m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J
UVIAflUB i
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UVIaltl~U~II1l iii d 0
UVIVI 1 UVIU1 1
11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1
ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1
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i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ
UVIVI 2 VIqlef~VILnll1t1i)4 4
21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5
23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G
24 n1iai1JGl1l1VlJVf1LBULB6
251t1iLLnilJ LabVIEW ~ 8
26 Principal Component Analysis (PCA) 8 ~1t1 ~ II
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31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d
32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0
322 oJGln1lVIflGlBJ 13
323 1~1iwt-JGln1lVIflGltlJ 14
33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15
332 t-JGln1aVIflGlBJ 15
323 11iW~Gln1lVIflGlBJ 19 41 tI CII
UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0
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422 ~Gln1iVWIGleJ~ 25
43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29
J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31
LeJn~1ieuroh~E~ 33
n91~n1It1in1f1 35
n1f1~1J1n n 36
d UVIVI 1
o UVIU1
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~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111
JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII
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I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl
~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II
~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l
1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or
Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1
- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I
12 1ltl5i1~Alla~lf1middotHn151~1J
1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U
d II 0 tf
2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ
3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll
13 lIaULlIlIa~lflJ~n1J1~1J
1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ
ElLflnVliElUn~
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2
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15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl
- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose
system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4
tJLGlnVI~tJUnG1
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LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~
III I r1 ltI 1
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fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V
_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1
UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v
d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
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tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
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12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
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eVe In the presence 0 reducing gas
6
(22)
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LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
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I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
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40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
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13
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3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
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mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
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2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
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allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
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MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
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I
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
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5
O~ o
200
400
600
800
0 0 20
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0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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ii
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O~shy
200
400
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o 20
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0 o
200
400
600
800
T
ime
(sec
) T
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(seo
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ne (
sec)
T
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(sec
)
No
9 N
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N
o12
2
22
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2
1
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0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
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0
Tim
e (s
ec)
Tim
e (s
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Tim
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00
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200
400
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200
400
600
800
Ti
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(sec
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Tim
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5
No
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Tim
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No
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TIm
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PCA CSP Row factor scores
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
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~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
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~ t I ~ I ~ ~ II
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m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm
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52 JElLlo11JtlU1Jt
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~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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UVIVI 1 UVIU1 1
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UVIVI 2 VIqlef~VILnll1t1i)4 4
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23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G
24 n1iai1JGl1l1VlJVf1LBULB6
251t1iLLnilJ LabVIEW ~ 8
26 Principal Component Analysis (PCA) 8 ~1t1 ~ II
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31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d
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332 t-JGln1aVIflGlBJ 15
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422 ~Gln1iVWIGleJ~ 25
43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29
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d UVIVI 1
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I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl
~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II
~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l
1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or
Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1
- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I
12 1ltl5i1~Alla~lf1middotHn151~1J
1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U
d II 0 tf
2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ
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13 lIaULlIlIa~lflJ~n1J1~1J
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15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl
- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose
system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4
tJLGlnVI~tJUnG1
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_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1
UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v
d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
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5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
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~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
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1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
RL
Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
~ 421 ijU~tlUn1iVlVl6itll
1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g
3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~
LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~
I I2 ----~----~-----r ~ -shy
I
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05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
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ii
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shy00
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200
400
600
800
o 20
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0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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50
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50
5
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0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
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Tim
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ec)
00
bull II
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602
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620
N
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0 0
200
400
600
800
0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
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Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
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200
400
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200
400
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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ec)
T
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(sec
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No
9
No
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1
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12
2
2
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15
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200
400
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0 0
200
400
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T
ime
(sec
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c)
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26
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20
N
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1
No2
N
o3
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4
~ 0
5
_
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N
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3 N
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15
15
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5
No
6 N
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8
2f
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r TI
me
(sec
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me
(sec
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me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
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S261
1
2f
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620
~ ~
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u
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00
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800
00
200
400
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silo
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00
200
400
600
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29
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PCA CSP Row factor scores
1-__---__
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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~
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30
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J tJVIVI 5
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1
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fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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52 JElLlo11JtlU1Jt
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
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Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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5
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200
400
600
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o 20
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0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
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ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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0
0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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00
bull II
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0 0
200
400
600
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0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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200
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200
400
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
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T
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(sec
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No
9
No
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1
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12
2
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200
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T
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(sec
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3 N
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5
No
6 N
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r TI
me
(sec
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me
(sec
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(sec
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TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
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S261
1
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620
~ ~
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u
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800
00
200
400
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silo
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200
400
600
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29
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1-__---__
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30
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
RL
Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
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400
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(sec
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32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
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5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
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Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
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7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
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41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
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fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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52 JElLlo11JtlU1Jt
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
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trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
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chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
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v V
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12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
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6
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LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
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tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
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2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
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iJ~ J I I I 1 0
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7
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(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
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L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
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2) Hybridization - based methods
8
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3) Sequencing - based methods
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
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33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
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- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
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Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
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eV
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ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
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LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
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16
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17
18
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BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
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l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
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conditioning -tilgt
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Computer
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~
LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~
I I2 ----~----~-----r ~ -shy
I
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05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~
-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
secta
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
~ 1
5
~
~
ii
I L-i
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-
-
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05
05
05
05 0
----
shy00
O~shy
200
400
600
800
o 20
0 40
0 80
0 80
0 20
0 40
0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
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(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
~
---~
15 ~
~0
~
-
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-
i0
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0
50
5 0
50
5
OL
0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
00
bull II
II
1
4
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41
c
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MQ
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bullbullbullbullbull
MQ
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----
TGS2
602
TG
S26
I 1
TGS2
620
N
0
--
--shy
---
2f
2
2
-
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------
1
15
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0
5
0-
0
0 0
a 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
)
Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
------
-
11
~~-shy
~ 5~~=-_~_
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5 ==
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0shy
00
o
200
400
600
800
0 20
0 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
T
ime
(sec
)
No
9
No
IO
No
1
No
12
2
2
2 2
_
tampamp
-shy --_1
IIfIII
15
15
15
~
II
~I_illiil_middot_middot
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middot~middotmiddot
middot-
shy6
~
gt
j gt
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05
05
0
5
0-middot
0
o
200
400
600
800
0 20
0 40
0 60
0 80
0 01
20
0 40
0 60
0 80
0 0
200
400
600
800
T
ime
(sec
) T
ime
(se
c)
Tim
e (s
ec)
Tim
e (s
ec)
14
tII-
cv
4
tI c
v ~
r~1Jt1 4
9 ~~9
16i
HYn
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11
6-3
fl1O
JflO
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Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1
12 f
lH
---M
Q-2
M
Q-3
M
Q-6
bullbullbullbullbull
MQ
-13
5
-T
OS
26
02
TO
S261
1 T
0526
20
N
-J
No
1
No2
N
o3
No
4
~ 0
5
_
No1
N
o2
No
3 N
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
RL
Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
~ 421 ijU~tlUn1iVlVl6itll
1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g
3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~
LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~
I I2 ----~----~-----r ~ -shy
I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~
-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
secta
z-
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__
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2
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
~ 1
5
~
~
ii
I L-i
C
-
-
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05
05
05
05 0
----
shy00
O~shy
200
400
600
800
o 20
0 40
0 80
0 80
0 20
0 40
0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
~
---~
15 ~
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~
-
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7
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-
i0
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0
50
5 0
50
5
OL
0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
00
bull II
II
1
4
tI
41
c
t d
Q
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ll
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MQ
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bullbullbullbullbull
MQ
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----
TGS2
602
TG
S26
I 1
TGS2
620
N
0
--
--shy
---
2f
2
2
-
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1
15
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0
5
0-
0
0 0
a 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
)
Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
------
-
11
~~-shy
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5 ==
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5
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0shy
00
o
200
400
600
800
0 20
0 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
T
ime
(sec
)
No
9
No
IO
No
1
No
12
2
2
2 2
_
tampamp
-shy --_1
IIfIII
15
15
15
~
II
~I_illiil_middot_middot
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middot~middotmiddot
middot-
shy6
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j gt
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05
05
0
5
0-middot
0
o
200
400
600
800
0 20
0 40
0 60
0 80
0 01
20
0 40
0 60
0 80
0 0
200
400
600
800
T
ime
(sec
) T
ime
(se
c)
Tim
e (s
ec)
Tim
e (s
ec)
14
tII-
cv
4
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12 f
lH
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Q-2
M
Q-3
M
Q-6
bullbullbullbullbull
MQ
-13
5
-T
OS
26
02
TO
S261
1 T
0526
20
N
-J
No
1
No2
N
o3
No
4
~ 0
5
_
No1
N
o2
No
3 N
o4
2 2
2 2
15
15
15
~
gt
gt
SV
_
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f ~~
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t i
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gt
gt
gt
gt
u u
u u
o 0
0
0
o ~ ~ ~ ~
0 ~ ~ ~ ~
0 ~
a ~ ~
0 ~ ~ ~ ~
~~
~~
~~
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No
5
No
6 N
o7
No
8
2f
2
~21
t
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tl
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5 ~
0
r-t-=t~
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o~ 20
0 40
0 80
0 silo
0~
zO
o 400
aO
o 80
0 0~
~
400
aOo
aOo
0 0 ~
a 60
0 80
0 ~~
r TI
me
(sec
) TI
me
(sec
) TI
me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
-T
GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
TGS2
620
~ ~
-
~ __
____
_ _ ~
lJiR
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f t
-
t
i gt
gt
gt
gt
u
U
U
M
00
200 ~ ~
800
00
200
400
800 ~
00 -
2ix
400
silo
aOo
00
200
400
600
800
TIm
e (s
ee)
TIm
e (s
ec)
TIm
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ec)
TIm
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ec)
atl
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10 r
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bull bull
29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun
PCA CSP Row factor scores
1-__---__
-1 -05 o 05 15 PCl
u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
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~
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2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1
30
Q ~ Q ~
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bull I I
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bull bull
J tJVIVI 5
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
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Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
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40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
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13
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3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
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mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
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2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
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allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
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MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
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---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
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5
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200
400
600
800
0 0 20
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0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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400
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200
400
600
800
T
ime
(sec
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ime
(seo
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ne (
sec)
T
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(sec
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No
9 N
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N
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2
22
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0
200
400
800
600
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0 80
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200
400
800
800
0 20
0 40
0 60
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0
Tim
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ec)
Tim
e (s
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00
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400
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200
400
600
800
Ti
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(sec
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Tim
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No
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Tim
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TIm
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
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3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of
Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and
Actuators Journal LUU]U
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LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~
III I r1 ltI 1
lilT 11 11 l~
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_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1
uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1
UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v
d UVIVI 2
21 mavmJ
fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1
iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1
l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl
sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii
1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV
~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull
llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115
Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~
mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl
trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]
UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull
chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]
Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el
1
Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV
5
22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij
VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj
Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)
Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]
LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
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tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
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eV
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17
18
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20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
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= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
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Sensor Array
oXoXoXoXO
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conditioning -tilgt
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Computer
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
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LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
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42 fl1iVlVl6itll1Vln~UmLVialJ
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
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15
15
15
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15
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5
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200
400
600
800
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60
0 80
0 0
0
200
400
600
800
T
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(sec
) T
ime
(sec
) T
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(sec
) T
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(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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200
400
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200
400
600
800
T
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(sec
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(seo
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sec)
T
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No
9 N
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N
o12
2
22
shy
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1
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0
200
400
800
600
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0 80
0 o
200
400
800
800
0 20
0 40
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0
Tim
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Tim
e (s
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0 0
200
400
600
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0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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00
o
200
400
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0 20
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200
400
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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T
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(sec
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No
9
No
IO
No
1
No
12
2
2
2 2
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15
15
15
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200
400
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20
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0 0
200
400
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T
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(sec
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bullbullbullbullbull
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20
N
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1
No2
N
o3
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4
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5
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N
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3 N
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15
15
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5
No
6 N
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8
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r TI
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(sec
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(sec
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(sec
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TIm
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ec)
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MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
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S261
1
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620
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800
00
200
400
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400
silo
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00
200
400
600
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atl
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
~
iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU
2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
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MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
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24
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No
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200
400
600
800
T
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(sec
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii
f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~
~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl
v V
eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl
tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22
Grain boundary Elec1ronbull Electron
ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)
12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)
CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)
~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~
LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i
eVe In the presence 0 reducing gas
6
(22)
brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~
~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U
m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI
LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u
23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ
Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS
tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry
231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase
tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun
1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~
I ~ I ~ ~ I I
neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)
2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J
fI11lJLVIlJ1pound1lJn~pound11iVl~nilil
3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu
232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n
leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1
tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln
iJ~ J I I I 1 0
fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I
etcl241 flLtlULtl
~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI
7
~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I
VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU
L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l
(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111
~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1
A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~
L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE
~
lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~
(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)
LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)
~ If Q ~4-
243 LWlftLIftn1U11~a191UW~LEULi)
L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U
1) Polymerase chain reaction (PCR) - based methods
L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl
iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru
L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn
L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy
Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length
Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU
2) Hybridization - based methods
8
VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1
~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail
Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U
3) Sequencing - based methods
nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~
LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu
cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~
ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~
LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl
t It 1 1 I
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2SLlIaUfla1J LabVIEW
1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU
1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln
~
tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~
Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1
26 Principal Component Analysis (PCA)
LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L
component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal
component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~
fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised
bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~
bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull
bull bull
UVIVI 3
rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ
OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
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Converter
Computer
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Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
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42 fl1iVlVl6itll1Vln~UmLVialJ
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
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15
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5
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200
400
600
800
0 0 20
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0 60
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0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
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400
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200
400
600
800
T
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(sec
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(seo
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sec)
T
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(sec
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No
9 N
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o12
2
22
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200
400
800
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200
400
800
800
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Tim
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Tim
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400
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200
400
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800
Ti
me
(sec
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(sec
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Tim
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No
5
No
6 N
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8
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Tim
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ec)
Tim
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No
9
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12
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T
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(sec
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(sec
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TIm
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ec)
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MQ-1
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o9
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0 N
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]
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3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
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Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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5 0
5 0
5
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200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
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200
400
600
800
o 20
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0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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50
5
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0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
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Tim
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00
bull II
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602
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N
0
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---
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0 60
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0 0
200
400
600
800
0 20
0 40
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
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Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
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00
o
200
400
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0 20
0 40
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200
400
600
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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ec)
T
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(sec
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No
9
No
IO
No
1
No
12
2
2
2 2
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15
15
15
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200
400
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0 20
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20
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0 0
200
400
600
800
T
ime
(sec
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(se
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14
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26
02
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20
N
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No
1
No2
N
o3
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4
~ 0
5
_
No1
N
o2
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3 N
o4
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15
15
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u u
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0
0
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0 ~ ~ ~ ~
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5
No
6 N
o7
No
8
2f
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r TI
me
(sec
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me
(sec
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me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
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620
~ ~
-
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____
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u
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00
200 ~ ~
800
00
200
400
800 ~
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400
silo
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00
200
400
600
800
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atl
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
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PCA CSP Row factor scores
1-__---__
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
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Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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5
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200
400
600
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o 20
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0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
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ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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0
0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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00
bull II
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0 0
200
400
600
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0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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200
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200
400
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
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T
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(sec
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No
9
No
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1
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12
2
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200
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T
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(sec
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3 N
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5
No
6 N
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r TI
me
(sec
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me
(sec
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(sec
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TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
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S261
1
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620
~ ~
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u
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800
00
200
400
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silo
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200
400
600
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29
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1-__---__
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30
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
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acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
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40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
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13
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3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
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mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
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2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
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allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
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MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
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I I2 ----~----~-----r ~ -shy
I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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~-~~~-r~~A~
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
~ 1
5
~
~
ii
I L-i
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05
05
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----
shy00
O~shy
200
400
600
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o 20
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0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
~
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15 ~
~0
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50
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50
5
OL
0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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00
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bullbullbullbullbull
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200
400
600
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0 20
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0 0
200
400
600
800
Ti
me
(sec
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me
(sec
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(sec
)
Tim
e (s
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No
5
No
6 N
o7
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8
21
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Tim
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Tim
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No
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200
400
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200
400
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T
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(sec
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1
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15
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5
No
6 N
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8
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r TI
me
(sec
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me
(sec
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me
(sec
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TIm
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I
MQ-1
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02
N
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0 N
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200
400
600
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
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PCA CSP Row factor scores
1-__---__
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
~
eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI
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30
Q ~ Q ~
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J tJVIVI 5
5 1lo11tl
J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J
elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J
m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
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1
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~ t I ~ I ~ ~ II
fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm
~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI
52 JElLlo11JtlU1Jt
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~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J
l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~
fIiUm1J
3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)
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bull bull
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OJ cv ( cv q laquoV d I OJt CV J 6
91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD
m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij
elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ
lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U
31 nTHihJ~Jenh~mbVimJ
lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m
IJ IJ
~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ
fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1
10
II C~ d CIt
32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l
LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)
U I U
f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment
Length Polymorphism)
ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ
3211 n11flMlflLtlULtl
1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S
(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM
LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65
tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1
LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11
13000 atJ~BU1Yi LtJUnm 30 U1Vi
4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11
Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11
10 U1Vi
5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El
ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1
10 U1Yi I ~ 1111 _co( ~ ~4
6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad
V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1
ltII
LViaaZa1poundJVltnBlJ()LBULeJ
11
3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
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17
18
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20
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
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= ~1E1rluqUU l
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1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
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Sensor Array
oXoXoXoXO
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
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LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
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15
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5
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200
400
600
800
0 0 20
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60
0 80
0 0
0
200
400
600
800
T
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(sec
) T
ime
(sec
) T
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(sec
) T
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(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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200
400
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o 20
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0 o
200
400
600
800
T
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(sec
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(seo
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ne (
sec)
T
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No
9 N
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o1I
N
o12
2
22
shy
2
1
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0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
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Tim
e (s
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0 0
200
400
600
800
0 20
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0 0
200
400
600
800
Ti
me
(sec
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me
(sec
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(sec
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Tim
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No
5
No
6 N
o7
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8
21
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00
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200
400
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0 20
0 40
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200
400
600
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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No
9
No
IO
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1
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12
2
2
2 2
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15
15
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05
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200
400
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20
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0 0
200
400
600
800
T
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(sec
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bullbullbullbullbull
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26
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20
N
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1
No2
N
o3
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4
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5
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N
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3 N
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15
15
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0
0
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5
No
6 N
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r TI
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(sec
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(sec
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(sec
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TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
o12
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S261
1
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2 2
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620
~ ~
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u
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00
200 ~ ~
800
00
200
400
800 ~
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400
silo
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00
200
400
600
800
TIm
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atl
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29
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30
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32
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
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7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
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8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
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9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
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10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
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51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
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34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
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15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
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jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
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40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
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13
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3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
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4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
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MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
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24
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I
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--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
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Time (sec)
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
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~
pound+
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0
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5
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200
400
600
800
0 0 20
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0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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ii
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O~shy
200
400
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0 o
200
400
600
800
T
ime
(sec
) T
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(seo
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ne (
sec)
T
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(sec
)
No
9 N
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N
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2
22
shy
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0
200
400
800
600
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0 80
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200
400
800
800
0 20
0 40
0 60
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0
Tim
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ec)
Tim
e (s
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400
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200
400
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800
Ti
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(sec
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Tim
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No
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Tim
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No
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TIm
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
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~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
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~ t I ~ I ~ ~ II
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52 JElLlo11JtlU1Jt
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)
t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~
v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa
I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj
~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3
lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I
VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1
~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml
j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel
tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA
ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter
mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37
euroNIfl1LtUaLilViI U1UtJ1lJflU
2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U
~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl
pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20
bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5
~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ
lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __
1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad
LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I
1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl
1 I
a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ
3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10
LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i
12
10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris
HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20
1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5
fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I
llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~
1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1
I ~ bull cJ ~ QI QQ
L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~
J _ aQcIi ~ ltQ
25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56
(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~
Cl~ 1 llJ1Vi)
4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel
mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~
jl 2 LLNlJ
2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic
acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn
t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J
Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi
4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11
nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel
40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250
Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20
Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl
6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu
J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI
3213 mithaLflfllV1llra
1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1
nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM
13
m~n
2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl
LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I
3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull
LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~
VI1LL~~iifl~flfln1~VIlIVl
4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10
mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5
bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f
5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II
flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j
~~L1flfllJLIJIJVI
3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~
tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1
2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056
formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~
3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi
4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium
thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ
5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1
~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~
322 Uiln1SVIVlilOt
~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)
I I I
mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~
14
~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2
V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1
G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with
arithmetic mean) lJ1~j~tI~ 32
CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2
100
SK1_3 SK2_3 SK2_1
SK2_2
2S
Zl
31
CN_1 SK1_1 SK1_2
91
323 1~1middotnl~iln1VIf1iltl~
LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy
Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~
LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI
VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1
mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U
LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU
33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl
LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J
bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj
m1l1rull11L~lIL~lJ~ tiltI
15
nTH(1 itllJlJl1 tJtnl
hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l
mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC
- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11
GC- MS
Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU
6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70
eV
_tot JCIII
~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1
ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU
UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1
LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil
LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2
ua 3 tJl1lJd1JtJ
16
itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v
LLiiI~ 3 1J111J1~U
17
18
11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2
LLat 3 fl1lJi~J
19
323 1)lUU~am1V1flaEll
LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~
V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy
BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~
tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq
nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1
tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~
41 0 eft t q
mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II
Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~
m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
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Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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5 0
5 0
5
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200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
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200
400
600
800
o 20
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0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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50
5
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0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
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Tim
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00
bull II
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602
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N
0
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---
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0 60
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0 0
200
400
600
800
0 20
0 40
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
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Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
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00
o
200
400
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0 20
0 40
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200
400
600
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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ec)
T
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(sec
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No
9
No
IO
No
1
No
12
2
2
2 2
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15
15
15
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200
400
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0 20
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20
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0 0
200
400
600
800
T
ime
(sec
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(se
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14
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26
02
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20
N
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No
1
No2
N
o3
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4
~ 0
5
_
No1
N
o2
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3 N
o4
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15
15
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u u
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0
0
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0 ~ ~ ~ ~
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5
No
6 N
o7
No
8
2f
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r TI
me
(sec
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me
(sec
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me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
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620
~ ~
-
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____
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u
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00
200 ~ ~
800
00
200
400
800 ~
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400
silo
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00
200
400
600
800
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atl
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
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PCA CSP Row factor scores
1-__---__
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI
l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
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41 0 eft t q
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt
= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
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3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
~ 421 ijU~tlUn1iVlVl6itll
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3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~
LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~
I I2 ----~----~-----r ~ -shy
I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~
-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
~
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ii
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05
05
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shy00
O~shy
200
400
600
800
o 20
0 40
0 80
0 80
0 20
0 40
0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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50
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50
5
OL
0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
00
bull II
II
1
4
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41
c
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MQ
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bullbullbullbullbull
MQ
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----
TGS2
602
TG
S26
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620
N
0
--
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---
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2
2
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0
0 0
a 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
)
Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
------
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11
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00
o
200
400
600
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0 20
0 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
T
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(sec
)
No
9
No
IO
No
1
No
12
2
2
2 2
_
tampamp
-shy --_1
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15
15
15
~
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05
05
0
5
0-middot
0
o
200
400
600
800
0 20
0 40
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0 01
20
0 40
0 60
0 80
0 0
200
400
600
800
T
ime
(sec
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(se
c)
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Tim
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14
tII-
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Q-2
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bullbullbullbullbull
MQ
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5
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26
02
TO
S261
1 T
0526
20
N
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No
1
No2
N
o3
No
4
~ 0
5
_
No1
N
o2
No
3 N
o4
2 2
2 2
15
15
15
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u u
u u
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0
0
o ~ ~ ~ ~
0 ~ ~ ~ ~
0 ~
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0 ~ ~ ~ ~
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No
5
No
6 N
o7
No
8
2f
2
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o~ 20
0 40
0 80
0 silo
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0 80
0 ~~
r TI
me
(sec
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me
(sec
) TI
me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
TGS2
620
~ ~
-
~ __
____
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f t
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t
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gt
gt
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u
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00
200 ~ ~
800
00
200
400
800 ~
00 -
2ix
400
silo
aOo
00
200
400
600
800
TIm
e (s
ee)
TIm
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ec)
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atl
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10 r
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bull bull
29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun
PCA CSP Row factor scores
1-__---__
-1 -05 o 05 15 PCl
u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
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~
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30
Q ~ Q ~
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bull I I
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J tJVIVI 5
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and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
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5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
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fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
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8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
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compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
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= ~1E1rluqUU l
d UVIVI 4
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-~ ell I c q oCtc ~ ~ 0 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
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Sensor Array
oXoXoXoXO
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conditioning -tilgt
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Converter
Computer
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Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
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II II
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
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(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
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42 fl1iVlVl6itll1Vln~UmLVialJ
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
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15
15
15
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15
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5
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200
400
600
800
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-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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200
400
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o 20
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200
400
600
800
T
ime
(sec
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(seo
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ne (
sec)
T
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(sec
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No
9 N
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N
o1I
N
o12
2
22
shy
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200
400
800
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0 80
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200
400
800
800
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0
Tim
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Tim
e (s
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00
bull II
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0 0
200
400
600
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0 0
200
400
600
800
Ti
me
(sec
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me
(sec
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(sec
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Tim
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No
5
No
6 N
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8
21
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0
Tim
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ec)
Tim
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No
9
No
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12
2
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200
400
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T
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(sec
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N
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1
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4
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N
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3 N
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5
No
6 N
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r TI
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(sec
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(sec
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(sec
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TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
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0 N
o11
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S261
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200
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29
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1-__---__
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30
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
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(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
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8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
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263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
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10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
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51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
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Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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I
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
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~
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5
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200
400
600
800
0 0 20
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-20
0 roo
60
0 80
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0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
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(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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400
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200
400
600
800
T
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(sec
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(seo
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ne (
sec)
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(sec
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9 N
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2
22
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200
400
800
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200
400
800
800
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0 40
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0
Tim
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Tim
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400
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200
400
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800
Ti
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(sec
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Tim
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No
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Tim
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TIm
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
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~ t I ~ I ~ ~ II
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52 JElLlo11JtlU1Jt
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
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Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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16
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17
18
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20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
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IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
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= ~1E1rluqUU l
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1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
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Sensor Array
oXoXoXoXO
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Computer
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MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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VRL = RL Vc ( RL + Rs) (41)
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23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
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42 fl1iVlVl6itll1Vln~UmLVialJ
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4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
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24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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--- MQ-2 - - - - - MQ-3
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TOS261l TGS2620
I
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Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
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15
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5
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200
400
600
800
0 0 20
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-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
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(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
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5
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200
400
600
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o 20
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0 o
200
400
600
800
T
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(sec
) T
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(seo
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ne (
sec)
T
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No
9 N
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N
o1I
N
o12
2
22
shy
2
1
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50
5
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0
0
200
400
800
600
0 20
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
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00
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0 60
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0 0
200
400
600
800
0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
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(sec
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Tim
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ec)
No
5
No
6 N
o7
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8
21
2
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00
o
200
400
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0 20
0 40
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0 0
200
400
600
800
0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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ec)
T
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(sec
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No
9
No
IO
No
1
No
12
2
2
2 2
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15
15
15
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05
0
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200
400
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0 20
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20
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0 0
200
400
600
800
T
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(sec
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(se
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bullbullbullbullbull
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26
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20
N
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1
No2
N
o3
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4
~ 0
5
_
No1
N
o2
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3 N
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2 2
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15
15
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u u
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0
0
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0 ~ ~ ~ ~
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5
No
6 N
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8
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r TI
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(sec
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me
(sec
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me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
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620
~ ~
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u
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00
200 ~ ~
800
00
200
400
800 ~
00 -
2ix
400
silo
aOo
00
200
400
600
800
TIm
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atl
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29
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
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3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
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5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
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techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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I I2 ----~----~-----r ~ -shy
I
I
05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
~
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ii
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shy00
O~shy
200
400
600
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o 20
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0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
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ne (
sec)
T
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(sec
)
No
9 N
oIO
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o1I
N
o12
2
22
shy
2
1
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~
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5
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0
0
200
400
800
600
0 20
0 40
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0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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00
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200
400
600
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200
400
600
800
Ti
me
(sec
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me
(sec
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(sec
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Tim
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5
No
6 N
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Tim
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Tim
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200
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T
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15
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5
No
6 N
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r TI
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(sec
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(sec
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(sec
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TIm
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29
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PCA CSP Row factor scores
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
~
eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI
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30
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J tJVIVI 5
5 1lo11tl
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
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~ t I ~ I ~ ~ II
fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm
~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI
52 JElLlo11JtlU1Jt
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~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J
l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy
20
dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl
allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~
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l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1
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41 0 eft t q
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Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II
IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q
mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II
n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl
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= ~1E1rluqUU l
d UVIVI 4
1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~
-~ ell I c q oCtc ~ ~ 0 ~
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
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Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
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- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
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l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
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23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
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l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
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11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
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I
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05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
~
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ii
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05
05
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shy00
O~shy
200
400
600
800
o 20
0 40
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0 20
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0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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50
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50
5
OL
0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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ec)
Tim
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00
bull II
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1
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bullbullbullbullbull
MQ
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----
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602
TG
S26
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620
N
0
--
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---
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2
2
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0
0 0
a 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
)
Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
------
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00
o
200
400
600
800
0 20
0 40
0 60
0 80
0 0
200
400
600
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
e (s
ec)
T
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(sec
)
No
9
No
IO
No
1
No
12
2
2
2 2
_
tampamp
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15
15
15
~
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05
0
5
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o
200
400
600
800
0 20
0 40
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0 01
20
0 40
0 60
0 80
0 0
200
400
600
800
T
ime
(sec
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(se
c)
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ec)
Tim
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14
tII-
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bullbullbullbullbull
MQ
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5
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26
02
TO
S261
1 T
0526
20
N
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No
1
No2
N
o3
No
4
~ 0
5
_
No1
N
o2
No
3 N
o4
2 2
2 2
15
15
15
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gt
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u u
u u
o 0
0
0
o ~ ~ ~ ~
0 ~ ~ ~ ~
0 ~
a ~ ~
0 ~ ~ ~ ~
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No
5
No
6 N
o7
No
8
2f
2
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0 40
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0 80
0 ~~
r TI
me
(sec
) TI
me
(sec
) TI
me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
TGS2
620
~ ~
-
~ __
____
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f t
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t
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gt
gt
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u
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00
200 ~ ~
800
00
200
400
800 ~
00 -
2ix
400
silo
aOo
00
200
400
600
800
TIm
e (s
ee)
TIm
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ec)
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TIm
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atl
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10 r
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bull bull
29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun
PCA CSP Row factor scores
1-__---__
-1 -05 o 05 15 PCl
u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
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~
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30
Q ~ Q ~
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ
41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~
LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~
~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135
(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0
~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~
1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41
Sensor Array
oXoXoXoXO
Chamber -IIo Signal
conditioning -tilgt
AID
Converter
Computer
- Recording -+
-Analysis
- Display
Sensor Applications
MQ-2 H2 LPG Methane CO Alcohol Smoke Propane
MQ-3 Alcohol Benzene Hexane LPG CO
MQ-6 G H2 Methane CO Alcohol
MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke
TGS2602 CH3 Ethanol NH3 Hydro~en sulphide
TGS2611 Methane 1so-butane Hydrogen Ethanol
TGS2620 Ethanol Hydrogen Iso-butane CO Methane
22
1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42
Vout
RL
Buffered
3NO _--4----lt1--0
l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41
VRL = RL Vc ( RL + Rs) (41)
11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U
23
1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii
LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU
II II
LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView
l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii
1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II
(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
v bull v
tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW
42 fl1iVlVl6itll1Vln~UmLVialJ
~ 421 ijU~tlUn1iVlVl6itll
1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g
3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II
11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II
4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47
5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView
24
25
422 ~am-SVlail4 J tV I O t 1 Gill d
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LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s
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I
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05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I
--- MQ-2 - - - - - MQ-3
MQ-6 bullbullbullbullbull MQ-l35 --TGS2602
TOS261l TGS2620
I
I
Time (sec)
-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll
fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n
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-----
---
No1
No
2 N
o3
No
4
2
2
2
2
~
15
15
15
~
15
-
2
~
pound+
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0
5 0
5 0
5 0
5
O~ o
200
400
600
800
0 0 20
0 40
0 60
0 80
0 0 0-
-20
0 roo
60
0 80
0 0
0
200
400
600
800
T
ime
(sec
) T
ime
(sec
) T
ime
(sec
) T
ime
(sec
)
No
5 N
o6
No
7 N
o8
2
2 2
2
--shy
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5
~
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ii
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shy00
O~shy
200
400
600
800
o 20
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0 20
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0 60
0 80
0 o
200
400
600
800
T
ime
(sec
) T
ime
(seo
) n
ne (
sec)
T
ime
(sec
)
No
9 N
oIO
N
o1I
N
o12
2
22
shy
2
1
5 ~
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5
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0
0
200
400
800
600
0 20
0 40
0 60
0 80
0 o
200
400
800
800
0 20
0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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00
bull II
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602
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0 0
200
400
600
800
0 20
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
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Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
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200
400
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200
400
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0 20
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0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
Tim
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T
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(sec
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No
9
No
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1
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12
2
2
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15
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200
400
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20
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0 0
200
400
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T
ime
(sec
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(se
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26
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20
N
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No
1
No2
N
o3
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4
~ 0
5
_
No1
N
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3 N
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15
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0
0
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5
No
6 N
o7
No
8
2f
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r TI
me
(sec
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me
(sec
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me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
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GS
26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
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620
~ ~
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____
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u
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00
200 ~ ~
800
00
200
400
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400
silo
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00
200
400
600
800
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29
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component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
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PCA CSP Row factor scores
1-__---__
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
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~
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30
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J tJVIVI 5
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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200
400
600
800
T
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(sec
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(sec
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(sec
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(sec
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5 N
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7 N
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T
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200
400
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Ti
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(sec
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(sec
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6 N
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0 60
0 80
0
Tim
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Tim
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200
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200
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T
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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No1
No
2 N
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4
2
2
2
2
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15
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200
400
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0
200
400
600
800
T
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(sec
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(sec
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(sec
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(sec
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No
5 N
o6
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7 N
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200
400
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T
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(sec
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sec)
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400
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Tim
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)
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(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU
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24
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(sec
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(sec
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5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
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fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
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7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
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singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)
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m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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~ t I ~ I ~ ~ II
fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
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Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
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BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
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2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
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3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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37
38
39
40
41
42
43
44
45
46
47
48
49
50
-----
---
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No
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4
2
2
2
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200
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T
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u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
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eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI
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30
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J tJVIVI 5
5 1lo11tl
J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J
elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J
m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
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~ t I ~ I ~ ~ II
fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
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m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm
~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI
52 JElLlo11JtlU1Jt
1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO
~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J
l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~
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3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
J
BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)
lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2
I
flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl
VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ
37
38
39
40
41
42
43
44
45
46
47
48
49
50
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200
400
600
800
0 20
0 40
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0 0
200
400
600
800
Ti
me
(sec
) Ti
me
(sec
) Ti
me
(sec
)
Tim
e (s
ec)
No
5
No
6 N
o7
No
8
21
2
------
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11
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0 40
0 60
0 80
0
Tim
e (s
ec)
Tim
e (s
ec)
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ec)
T
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(sec
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No
9
No
IO
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1
No
12
2
2
2 2
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tampamp
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15
15
15
~
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~I_illiil_middot_middot
-
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05
05
0
5
0-middot
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o
200
400
600
800
0 20
0 40
0 60
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20
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0 60
0 80
0 0
200
400
600
800
T
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(sec
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14
tII-
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12 f
lH
---M
Q-2
M
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M
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bullbullbullbullbull
MQ
-13
5
-T
OS
26
02
TO
S261
1 T
0526
20
N
-J
No
1
No2
N
o3
No
4
~ 0
5
_
No1
N
o2
No
3 N
o4
2 2
2 2
15
15
15
~
gt
gt
SV
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gt
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u u
u u
o 0
0
0
o ~ ~ ~ ~
0 ~ ~ ~ ~
0 ~
a ~ ~
0 ~ ~ ~ ~
~~
~~
~~
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No
5
No
6 N
o7
No
8
2f
2
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o~ 20
0 40
0 80
0 silo
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aO
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0 0~
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400
aOo
aOo
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a 60
0 80
0 ~~
r TI
me
(sec
) TI
me
(sec
) TI
me
(sec
)
TIm
e (s
ec)
I
MQ-1
35
-T
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26
02
N
o9
No1
0 N
o11
N
o12
TG
S261
1
2f
2 2
l l
TGS2
620
~ ~
-
~ __
____
_ _ ~
lJiR
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f t
-
t
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gt
gt
gt
u
U
U
M
00
200 ~ ~
800
00
200
400
800 ~
00 -
2ix
400
silo
aOo
00
200
400
600
800
TIm
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ee)
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atl
-ri 4
10 r
1Wlf
llJff
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bull bull
bull bull
29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun
PCA CSP Row factor scores
1-__---__
-1 -05 o 05 15 PCl
u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
~
eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI
2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1
30
Q ~ Q ~
m~nvm)unG1
el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~
bull I I
ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I
111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI
bull bull
J tJVIVI 5
5 1lo11tl
J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J
elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J
m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU
1
a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU
1
lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl
~ t I ~ I ~ ~ II
fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El
fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J
m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm
~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI
52 JElLlo11JtlU1Jt
1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO
~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J
l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~
fIiUm1J
3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~
tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1
32
4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l
J
BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n
1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)
lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2
I
flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl
VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ
37
38
39
40
41
42
43
44
45
46
47
48
49
50
~ 0
5
_
No1
N
o2
No
3 N
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2 2
2 2
15
15
15
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u u
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0
0
o ~ ~ ~ ~
0 ~ ~ ~ ~
0 ~
a ~ ~
0 ~ ~ ~ ~
~~
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No
5
No
6 N
o7
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8
2f
2
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o~ 20
0 40
0 80
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r TI
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(sec
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(sec
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(sec
)
TIm
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0 N
o11
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1
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l l
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620
~ ~
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u
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00
200 ~ ~
800
00
200
400
800 ~
00 -
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400
silo
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00
200
400
600
800
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29
43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1
m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal
component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal
component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised
LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~
LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun
PCA CSP Row factor scores
1-__---__
-1 -05 o 05 15 PCl
u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA
lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ
Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _
S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf
S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i
~
eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI
2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1
30
Q ~ Q ~
m~nvm)unG1
el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~
bull I I
ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I
111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI
bull bull
J tJVIVI 5
5 1lo11tl
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vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116
(1992)
7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor
approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and
Actuators B Chemical 123 1101-1106 (2007)
8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of
odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85
263-269 (2002)
9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor
recorder for multicomponent odor using recipe exploration method based on
singular value decomposition Sensors Journal IEEE 3 468-474 (2003)
10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor
identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80
51-58 (2001)
11 A Szczurek and M Maciejewska Relationship between odour intensity assessed
by human assessor and TGS sensor array responsemiddot Sensors and Actuators B
Chemical 106 13-19 (2005)
34
12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719
16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull
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1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in
vitro or in vivo ingestion of raw or heated garlic using electronic nose gas
chromatography and sensory analysis International Journal of Food Science amp
Technology 43 130-139 (2008)
2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of
Agricultural and Food Chemistry 37 725-730 (1989)
3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic
and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)
4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of
Nutrition 136 716S-725S (2006)
5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer
analysis of volatile sulfur compounds in garlic essential oils Application of the mass
fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)
6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components
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12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile
compounds of []Fujj apples in relation to harvest date and cold storage technoLogy
A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)
13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal
Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)
Food Chemistry 86 127-130 (2004)
14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food
beverages and perfumes by W03 thin-film sensors array and pattern recognition
techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)
15 GC-MS (Gas Chromatography - Mass Spectrometry)
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