21 .11. 2556 - ubon ratchathani university

58
21 "'l'Mfl1fIJ1J ".1"1. 2556

Upload: others

Post on 15-Mar-2022

11 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: 21 .11. 2556 - Ubon Ratchathani University

21 lMfl1fIJ1J 11 2556

Administrator
Rectangle
Administrator
Oval

Development of electronic nose system to classify garlic type

Ifl~Jn1~1~EJmflrUVlUn~VIUUn1~1~EJ1nnn~V1U1~EJUVl11V1EJ1~EJnUfl~1n1il

Administrator
Rectangle
Administrator
Rectangle

tltltltlUflClJ VlUl1f1fliJ G1lJ ijru f11f111l11mmjlJBL~nVIjtlilm1u(l~1V1jfllJ1Jlf1lJ f1W~bull li

1mmjlJf11G1~i lJVn1V1C11~C1mlLVIW t1JR1U1J~IllL~tl1nUn1jrrVll1Jlj~UUllJnBL~nVIjtliln~~ LiJ1Jth~1C1ttt1~11~1

tltltltlUflW Vlj f1jf1j fI1~n(l1Jljfl ml1jpoundJth~~lfllf111l1-W1Jofl11G1~i f1ClJ~1V1Ulf11G1~i lJvn1V1Ul~CJ Ln~~j 1l1G1lli vtlViR1LLU ~IllU(l~f111lJ~1lJiitlt 1Jn1jG1~lJ(llCJYllJw~ L~1J LtIvhCJ~LG1lJtI

lJl tltltltlUflWeurolllljaVIjJWj ~Jl1JfIJ f1W~LflilIJf11G1~i lJVn1V1Ul~CJ~U(ljl1l01j ~l1J

LL(l~L-WCJlWCJ1CJ1lJlht Vin1j1~LL(l~1 LfIjl~l11itl(lv11mfl~t1J GC-MS L U1J t1lv1v11CJ~ If _4 CIIIQ 1J CIt cv

tltltltlUflWmlljmL(l~UfI(llm fllf111l11f11mjlJLfllJu(l~~HU1Vl(ltllJ lJVll1V1Ul(lCJ ~~ If I ell I 1 I~

~U(ljl1lO11J VI LVlfl11lJtI4LfIjl~Vl LL(l~1l1mVl(ltl1Jn1j LIJ LfIjtlJ GC-MS L u1JtlUl-3Vl

tltltltlUflClJ1J1CJ~~r41JG B1JVIi-31lJ U~1J1CJ1J~ 11JVI~1lCJ unimilfllf111l1 ~

1mmj1l1vHt1u(l~Eh~nVIjtlilna flClJ~1i11mjllil1~~i lJVl11V1CJ1~CJ~U(ljlIJtnj ~Ii1CJIijVlVll1G1~

~tlmruLL(l~~llIAj~UUllnB L~ nVIjtlilnaL~l1Ijjru

~ilflHm1iv (o1tt11 VlV) mw~lJ1UU~1JnihanVliiln~Li~ij1uunwlJtimLViv1Jv

(o1tt1elmqtt) Development of electronic nose system to classify garlic

type

Lnmm1JLthJmjllVl~nlmltl~I111llJ1lJtILVI PllVIV 1lJLL~rtlijTll1tJrVI11m~ Ln~lJl~

lii1lJ1lJ1J1nvtii n~VfiIJlr1V1 ~lTll1tJrLL~r1lijVlnijVlr1 nVlr1EJ~1EJwlJtivh1l1~u~lofllii1lJ1lJhi jtlEJtI~~U1tyVl11lJm~ L~in~tlTl1ltJaVI11m~Lnm~1m~fl111JtlJm mLViEJ1J (Allium

) ~I - JIII ou tI 1 I r 1Sativum L m ulJelrNaJlVI1~m~ Lnm~1llJVlVllJJVI~Uj Lofln1Jn~ ~~UutyVl1 LlJm~Lrtln~tl lJ

n~LViEJ1Jijtll~tI~ntlUJtl~irL~tlfllJm1J1C1Jv1~~1I1n lV1EJij 5-2 -prope nyl-L -cysteine

sulfuroxide (allin) tl~iiJ 85 ~I~JtJrlJitln~lJLLr~CIJrrnliClJVI1Jj1o1WJtlJnLViEJ1I1V1EJn~1J JtlJmLViEJ1JLOVll1nmv1LilJl~~ allinase 1t1fin~EJ1nU allin 11l1LOVl diallyl thiosulfinate

(allicin) i1J1I1 LLr L~tl~l1nmLViEJ1JLLIJirWlJq~ij~ LLrn~lJv1LL~lnIJi1JnU11l1mLViEJlIU11

ilJqLjlJv1ijEJlIJtl~~IJ~1nfltlEhJ1I1n Bn~J1lJ~Jl~1V1m~lItl1V11~~tI~ntlumjn~L~ tln1i I I t a 1IIIt

mLVI EJlIWlJfiVlJl eHmLVI1lJlJLWifl1Uflll flC1Jo1W LLa~1J~U1JlVlL1JlJLtlna n~CIJLtlW1Jl1Jtll1

~lJfh LLJltlJmL~tln~tlmLVivlIll1JljJnUfl1111~tlJmJtlJ~IJnnflrlJaJ~tlJtl1(EJrhalJalJ~1n ~~1 lV1ElLtlW1tlV1J~lllJt1~~U1JVI~~~1mJVI LJ Jlm~1L~~11l1njLViEJ1J~1nIJi1JtIiLVIPI

Vla1nVla1E1ilJ~~n~JLu11111lJtILVIPllV1EJlii1lJ1lJ1I1n Bn~~aJLnVl1tyVl1mLLtllJa1~VI~tltlrtllltl1J 11 I tIshy

mLVlEllI~1EJWlJtiVILulJVIJltlJmiLLr1fl1~lVl1EJmLVlEJlI~1EJWlJ6V1 LnaLflEJJn1JtlnVl1EJ LVlEJ nJl v bull

LL~1m~lii1 LLlJnilJ~mLViElllrlJ~~1111fIj1 LLlJntltlmt1lJmjllLVlq 1 1~Jl111tl1~m~LnUL~El1V1~tl 1 1 f 3 I tI

l1LLlJnJl11JLLVlrlVl1I1Jtl~ll1Jfi~I~1111~flVI LVI~1nJlJ1V1V1itllJ1V1lJn ~1V1IJmLVlEllI LlJ iLVIfII LVlEJ ~ 1 v

rlJ~LLtiJtltlnl~LjlJ 3 tILnVlJl111tl1~mjLnUL~El1 l~LLri WlJ~LU1 tl1~miLnUL~El1tl~tlJ-d1J 75 - 90 1lJ l~LLri ilJqfll~~Lntt ilJ~nr1Jtl1~m~LnUL~EJ1tl~tlJ-d1J 100 - 120 11J l~LLri ilJ~

1 4 tI G d ~ tll I

U1~U1~ LLrll1J~LUEJJ LVllI I

LLrWlJ~VllJn tl1~miLnIJLnEl1 150 1lJIlJL VlLLn W1JqW1I1LLrWlJ~ bull I ~ cal -II I 1

LUJ~1EJ LLJl~1nm~~UfllJEIJ wWIJJtl~rVlLWEIJWmnEl1nU ~CIJallUJlJtl~~1iiLVlEIJtl~mLVI EJlI VlEI middot 1 a I d J gtAT -J 0 I 1

LLJltlEJ1J nJl111 L 1JlJLtl~EJ1n~1V1W~IJ~u IflVl~~1LLlJnn~LVI EJlIJl1~~1EIWlJqVlllr n~CIJo1EJlJin

Ln~LfliEl~nlJ

~1n~CIJ~lIumlJm~Jli1ll~1~LflijJtll~~niiL~nVlitlijm (Electronic nose or Gas a I I tI tI-- sensor or 0 dor sensor) tI~LnVl~1~nJ1Jl11J1V1fl1111Jl1lJVl1lJJl1EJ L1JlLL itJlJJl111 )mEJ1Lflll

1 4 c cCJ 11 tl J iVl11J~1~nJIJl11J1nUfl1111LJ1IJlJIeuroJI~1iLflllVIL1J1Jtllfl ~n tlIJ L lJm~VlIJli1 ~1V1 N1lEllI

v 4J 0 _

LL1J1fl1111flVlVI~W~lJ1iIJIJ~1LLlJnWlJtim L VI EllIVl1EJmiJli1~nr1JJtlJmLVlEllIJl1~~1E1lllJ6 v bull

~1E1~lJniiLifnVlitlijna L~m~1nLtllJ~UlJ1V1mj-aIJiftllJLVl1J1LLrim~i~lJ1LTitllj11111gnu miLn~Jl~llJtIiLVlPI lV1EJ~uum~rlJlJtln~1n~1mlJnTIlii1LLlJnilJ~niLViEl1JLL~1 aJ~1111~fll-ij

iv

A Q_A

ItLU~U1fj11i1V

t U-11U1ieJilL~tln t -tilJna ~nll1tlilm1lfil~C111~-1~1111t Um1~11in~Utl-1mtLVi eJlJ i II a

~

~ bull tJ ~

41 IVA

~~eJt ~IflJntlLnll1tlUnm1eJ~1 (7 I1U~) lIlJm1~tlUC1Utl-1~tlC111 1tntlUlI1-1LfllJlI~1-1nUlJ1 ~

tJ1tntlU-1tUU~U1j1 ItUU1V1-rU1~n~U1nn1tLVieJlJtQntltlnLLUUt~LjU1tUUtJ~L~tlU~1 fl1UfllJmIlV1Utljtl1mf3tt~~1UL~ULiltliJl1eJ~lJl1l~tl1mf3t Utln1nilU1-1I1~tijmII1~LlfeJjj ~

I I ~ j ~ bull Jamp~ I lot Utlj ~CUVIlJlJLVi tl LVILn~fl11lJfI1~LfltlU LU1t~UlI~1l1~~ C1tylPCU Ln1111i11nLilULiltl1U~t~1l1 LI1l

1iItQnUtJ-1LiJUatyty1CU~~lJItlUt~1mIi~L~UjtUfltllJl1L~tli1~eJt-ti~tJn1ru ADC (Analog to

Digital Conversion) 1iI1mrULlIflilflm1tJ~tlJ1jjatyty1CUUtm~1Lfl~1t~lI1jaa~ (MATLAB)

tOn111lJ1t mUm~VI1flW~m~CUtL1lVi1tlftljjjlJItlUaUtljll1jL~~Tm~1iI1nlJnaL~nll1tlilmllftlj~ ~

mtLVieJlJulJitI1il~ fhflcu~m~JWtm~aUt111lJ1tmUmI~1uUn~UtimtLVieJlJ~1eJLlIflilfl Principal component analysis (PCA) m~~1-11Ulftl-1~tUU1~ ~tUUi~L~Utl1J ut~tUU

tJ~tlJ1jj~jVIlJ~1iItV11m~L11tllJlJitluti~m~~1eJltJ1umlJfltllJl1L~tlivi~WJU1iULtl~UU 1 tJIumlJ LabVIEW ujtlItUUijfl11lJalJPruu~1 t UfUmIlI~tl-1t111~1tl~h~mtLVieJlJt U

tJItLlIf3tll1eJ1n 3 UVIrl~ L~url tleJ1~lflJjtleJ f3t~C1tLn~ tlJ1tJ1~ tllJlI~IJIamp Ut tlu1Uluj iN1ViU ~-1LtJUUVIrl~tJnmtLVieJlJvi1i~tlLaeJ-1 lJ1V11mIL~UtllJC11lItLVIeJ~1eJ~tUU1iIlJn

~ ~ ~ ~

aL~nll1tliln~viai1~1U l~eJ1iItLmElULViElUUt1JI~1atlUtl~fltJ1 tntlU t UaT~ItLVIeJtJtl~ulJit ~1mh~~1 ElLfl~tl-1 GC-MS vi~tl~tJ~jijmIlftlgt1n1f111111mmIlJLflijut~~u1~tltllJ flWt

1f3t1nIllJf3t1alJlimiddot lJVI11l1El1~El~U~1lffj1ii l~ElmciLmElULViElUltltltlUIflIlJ11~ILLmlJL~tltn~1 fl11lJUlJlnIJi1~lftl~a1lri1ulftl~tl~fitJltntlUtUn~Ulftl~mtLVieJlJulJit~Uti Utln1nilutl1L~tlLtJU ml~Uvu-hmtLViEllJ~1tlEi1~vi111lJ1l11lC1tlUaULtJUa1eJ~UtiviUlJlnIJi1gt1nU ~1tlV1-11i1 tQn-u1LtJVI1

tllJ~UfjmIlJ CU n1fl1111~UfjmalJli flCUt1l1El1f3t1alJli lJ1l1El1~ElLn~ImalJli tllJm~ I

1nmI1Lfll1tVIUulJit16mn111lJ1LmElULViElUnUL~tltJItWUml~1uUn~UfimtLViEllJ~1eJ ~

1JnaL~nll~tliln~ utVi1mliWJU1ItUU1~tvLVIlJ1talJnUm~t -tij1U~~lJitlLtJ Utln1nilu~1t U

~1U1iEliltVi1mltn~1V11UU1l11~L~tlll~tl~~wJU1lJnaL~nllltliln~~U 11UUihVl-rU1~a1I ~ ~

ItLVI El1nnItLViEllJiUt -ti-11L1Ltl~8nJI1El

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 2: 21 .11. 2556 - Ubon Ratchathani University

Development of electronic nose system to classify garlic type

Ifl~Jn1~1~EJmflrUVlUn~VIUUn1~1~EJ1nnn~V1U1~EJUVl11V1EJ1~EJnUfl~1n1il

Administrator
Rectangle
Administrator
Rectangle

tltltltlUflClJ VlUl1f1fliJ G1lJ ijru f11f111l11mmjlJBL~nVIjtlilm1u(l~1V1jfllJ1Jlf1lJ f1W~bull li

1mmjlJf11G1~i lJVn1V1C11~C1mlLVIW t1JR1U1J~IllL~tl1nUn1jrrVll1Jlj~UUllJnBL~nVIjtliln~~ LiJ1Jth~1C1ttt1~11~1

tltltltlUflW Vlj f1jf1j fI1~n(l1Jljfl ml1jpoundJth~~lfllf111l1-W1Jofl11G1~i f1ClJ~1V1Ulf11G1~i lJvn1V1Ul~CJ Ln~~j 1l1G1lli vtlViR1LLU ~IllU(l~f111lJ~1lJiitlt 1Jn1jG1~lJ(llCJYllJw~ L~1J LtIvhCJ~LG1lJtI

lJl tltltltlUflWeurolllljaVIjJWj ~Jl1JfIJ f1W~LflilIJf11G1~i lJVn1V1Ul~CJ~U(ljl1l01j ~l1J

LL(l~L-WCJlWCJ1CJ1lJlht Vin1j1~LL(l~1 LfIjl~l11itl(lv11mfl~t1J GC-MS L U1J t1lv1v11CJ~ If _4 CIIIQ 1J CIt cv

tltltltlUflWmlljmL(l~UfI(llm fllf111l11f11mjlJLfllJu(l~~HU1Vl(ltllJ lJVll1V1Ul(lCJ ~~ If I ell I 1 I~

~U(ljl1lO11J VI LVlfl11lJtI4LfIjl~Vl LL(l~1l1mVl(ltl1Jn1j LIJ LfIjtlJ GC-MS L u1JtlUl-3Vl

tltltltlUflClJ1J1CJ~~r41JG B1JVIi-31lJ U~1J1CJ1J~ 11JVI~1lCJ unimilfllf111l1 ~

1mmj1l1vHt1u(l~Eh~nVIjtlilna flClJ~1i11mjllil1~~i lJVl11V1CJ1~CJ~U(ljlIJtnj ~Ii1CJIijVlVll1G1~

~tlmruLL(l~~llIAj~UUllnB L~ nVIjtlilnaL~l1Ijjru

~ilflHm1iv (o1tt11 VlV) mw~lJ1UU~1JnihanVliiln~Li~ij1uunwlJtimLViv1Jv

(o1tt1elmqtt) Development of electronic nose system to classify garlic

type

Lnmm1JLthJmjllVl~nlmltl~I111llJ1lJtILVI PllVIV 1lJLL~rtlijTll1tJrVI11m~ Ln~lJl~

lii1lJ1lJ1J1nvtii n~VfiIJlr1V1 ~lTll1tJrLL~r1lijVlnijVlr1 nVlr1EJ~1EJwlJtivh1l1~u~lofllii1lJ1lJhi jtlEJtI~~U1tyVl11lJm~ L~in~tlTl1ltJaVI11m~Lnm~1m~fl111JtlJm mLViEJ1J (Allium

) ~I - JIII ou tI 1 I r 1Sativum L m ulJelrNaJlVI1~m~ Lnm~1llJVlVllJJVI~Uj Lofln1Jn~ ~~UutyVl1 LlJm~Lrtln~tl lJ

n~LViEJ1Jijtll~tI~ntlUJtl~irL~tlfllJm1J1C1Jv1~~1I1n lV1EJij 5-2 -prope nyl-L -cysteine

sulfuroxide (allin) tl~iiJ 85 ~I~JtJrlJitln~lJLLr~CIJrrnliClJVI1Jj1o1WJtlJnLViEJ1I1V1EJn~1J JtlJmLViEJ1JLOVll1nmv1LilJl~~ allinase 1t1fin~EJ1nU allin 11l1LOVl diallyl thiosulfinate

(allicin) i1J1I1 LLr L~tl~l1nmLViEJ1JLLIJirWlJq~ij~ LLrn~lJv1LL~lnIJi1JnU11l1mLViEJlIU11

ilJqLjlJv1ijEJlIJtl~~IJ~1nfltlEhJ1I1n Bn~J1lJ~Jl~1V1m~lItl1V11~~tI~ntlumjn~L~ tln1i I I t a 1IIIt

mLVI EJlIWlJfiVlJl eHmLVI1lJlJLWifl1Uflll flC1Jo1W LLa~1J~U1JlVlL1JlJLtlna n~CIJLtlW1Jl1Jtll1

~lJfh LLJltlJmL~tln~tlmLVivlIll1JljJnUfl1111~tlJmJtlJ~IJnnflrlJaJ~tlJtl1(EJrhalJalJ~1n ~~1 lV1ElLtlW1tlV1J~lllJt1~~U1JVI~~~1mJVI LJ Jlm~1L~~11l1njLViEJ1J~1nIJi1JtIiLVIPI

Vla1nVla1E1ilJ~~n~JLu11111lJtILVIPllV1EJlii1lJ1lJ1I1n Bn~~aJLnVl1tyVl1mLLtllJa1~VI~tltlrtllltl1J 11 I tIshy

mLVlEllI~1EJWlJtiVILulJVIJltlJmiLLr1fl1~lVl1EJmLVlEJlI~1EJWlJ6V1 LnaLflEJJn1JtlnVl1EJ LVlEJ nJl v bull

LL~1m~lii1 LLlJnilJ~mLViElllrlJ~~1111fIj1 LLlJntltlmt1lJmjllLVlq 1 1~Jl111tl1~m~LnUL~El1V1~tl 1 1 f 3 I tI

l1LLlJnJl11JLLVlrlVl1I1Jtl~ll1Jfi~I~1111~flVI LVI~1nJlJ1V1V1itllJ1V1lJn ~1V1IJmLVlEllI LlJ iLVIfII LVlEJ ~ 1 v

rlJ~LLtiJtltlnl~LjlJ 3 tILnVlJl111tl1~mjLnUL~El1 l~LLri WlJ~LU1 tl1~miLnUL~El1tl~tlJ-d1J 75 - 90 1lJ l~LLri ilJqfll~~Lntt ilJ~nr1Jtl1~m~LnUL~EJ1tl~tlJ-d1J 100 - 120 11J l~LLri ilJ~

1 4 tI G d ~ tll I

U1~U1~ LLrll1J~LUEJJ LVllI I

LLrWlJ~VllJn tl1~miLnIJLnEl1 150 1lJIlJL VlLLn W1JqW1I1LLrWlJ~ bull I ~ cal -II I 1

LUJ~1EJ LLJl~1nm~~UfllJEIJ wWIJJtl~rVlLWEIJWmnEl1nU ~CIJallUJlJtl~~1iiLVlEIJtl~mLVI EJlI VlEI middot 1 a I d J gtAT -J 0 I 1

LLJltlEJ1J nJl111 L 1JlJLtl~EJ1n~1V1W~IJ~u IflVl~~1LLlJnn~LVI EJlIJl1~~1EIWlJqVlllr n~CIJo1EJlJin

Ln~LfliEl~nlJ

~1n~CIJ~lIumlJm~Jli1ll~1~LflijJtll~~niiL~nVlitlijm (Electronic nose or Gas a I I tI tI-- sensor or 0 dor sensor) tI~LnVl~1~nJ1Jl11J1V1fl1111Jl1lJVl1lJJl1EJ L1JlLL itJlJJl111 )mEJ1Lflll

1 4 c cCJ 11 tl J iVl11J~1~nJIJl11J1nUfl1111LJ1IJlJIeuroJI~1iLflllVIL1J1Jtllfl ~n tlIJ L lJm~VlIJli1 ~1V1 N1lEllI

v 4J 0 _

LL1J1fl1111flVlVI~W~lJ1iIJIJ~1LLlJnWlJtim L VI EllIVl1EJmiJli1~nr1JJtlJmLVlEllIJl1~~1E1lllJ6 v bull

~1E1~lJniiLifnVlitlijna L~m~1nLtllJ~UlJ1V1mj-aIJiftllJLVl1J1LLrim~i~lJ1LTitllj11111gnu miLn~Jl~llJtIiLVlPI lV1EJ~uum~rlJlJtln~1n~1mlJnTIlii1LLlJnilJ~niLViEl1JLL~1 aJ~1111~fll-ij

iv

A Q_A

ItLU~U1fj11i1V

t U-11U1ieJilL~tln t -tilJna ~nll1tlilm1lfil~C111~-1~1111t Um1~11in~Utl-1mtLVi eJlJ i II a

~

~ bull tJ ~

41 IVA

~~eJt ~IflJntlLnll1tlUnm1eJ~1 (7 I1U~) lIlJm1~tlUC1Utl-1~tlC111 1tntlUlI1-1LfllJlI~1-1nUlJ1 ~

tJ1tntlU-1tUU~U1j1 ItUU1V1-rU1~n~U1nn1tLVieJlJtQntltlnLLUUt~LjU1tUUtJ~L~tlU~1 fl1UfllJmIlV1Utljtl1mf3tt~~1UL~ULiltliJl1eJ~lJl1l~tl1mf3t Utln1nilU1-1I1~tijmII1~LlfeJjj ~

I I ~ j ~ bull Jamp~ I lot Utlj ~CUVIlJlJLVi tl LVILn~fl11lJfI1~LfltlU LU1t~UlI~1l1~~ C1tylPCU Ln1111i11nLilULiltl1U~t~1l1 LI1l

1iItQnUtJ-1LiJUatyty1CU~~lJItlUt~1mIi~L~UjtUfltllJl1L~tli1~eJt-ti~tJn1ru ADC (Analog to

Digital Conversion) 1iI1mrULlIflilflm1tJ~tlJ1jjatyty1CUUtm~1Lfl~1t~lI1jaa~ (MATLAB)

tOn111lJ1t mUm~VI1flW~m~CUtL1lVi1tlftljjjlJItlUaUtljll1jL~~Tm~1iI1nlJnaL~nll1tlilmllftlj~ ~

mtLVieJlJulJitI1il~ fhflcu~m~JWtm~aUt111lJ1tmUmI~1uUn~UtimtLVieJlJ~1eJLlIflilfl Principal component analysis (PCA) m~~1-11Ulftl-1~tUU1~ ~tUUi~L~Utl1J ut~tUU

tJ~tlJ1jj~jVIlJ~1iItV11m~L11tllJlJitluti~m~~1eJltJ1umlJfltllJl1L~tlivi~WJU1iULtl~UU 1 tJIumlJ LabVIEW ujtlItUUijfl11lJalJPruu~1 t UfUmIlI~tl-1t111~1tl~h~mtLVieJlJt U

tJItLlIf3tll1eJ1n 3 UVIrl~ L~url tleJ1~lflJjtleJ f3t~C1tLn~ tlJ1tJ1~ tllJlI~IJIamp Ut tlu1Uluj iN1ViU ~-1LtJUUVIrl~tJnmtLVieJlJvi1i~tlLaeJ-1 lJ1V11mIL~UtllJC11lItLVIeJ~1eJ~tUU1iIlJn

~ ~ ~ ~

aL~nll1tliln~viai1~1U l~eJ1iItLmElULViElUUt1JI~1atlUtl~fltJ1 tntlU t UaT~ItLVIeJtJtl~ulJit ~1mh~~1 ElLfl~tl-1 GC-MS vi~tl~tJ~jijmIlftlgt1n1f111111mmIlJLflijut~~u1~tltllJ flWt

1f3t1nIllJf3t1alJlimiddot lJVI11l1El1~El~U~1lffj1ii l~ElmciLmElULViElUltltltlUIflIlJ11~ILLmlJL~tltn~1 fl11lJUlJlnIJi1~lftl~a1lri1ulftl~tl~fitJltntlUtUn~Ulftl~mtLVieJlJulJit~Uti Utln1nilutl1L~tlLtJU ml~Uvu-hmtLViEllJ~1tlEi1~vi111lJ1l11lC1tlUaULtJUa1eJ~UtiviUlJlnIJi1gt1nU ~1tlV1-11i1 tQn-u1LtJVI1

tllJ~UfjmIlJ CU n1fl1111~UfjmalJli flCUt1l1El1f3t1alJli lJ1l1El1~ElLn~ImalJli tllJm~ I

1nmI1Lfll1tVIUulJit16mn111lJ1LmElULViElUnUL~tltJItWUml~1uUn~UfimtLViEllJ~1eJ ~

1JnaL~nll~tliln~ utVi1mliWJU1ItUU1~tvLVIlJ1talJnUm~t -tij1U~~lJitlLtJ Utln1nilu~1t U

~1U1iEliltVi1mltn~1V11UU1l11~L~tlll~tl~~wJU1lJnaL~nllltliln~~U 11UUihVl-rU1~a1I ~ ~

ItLVI El1nnItLViEllJiUt -ti-11L1Ltl~8nJI1El

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 3: 21 .11. 2556 - Ubon Ratchathani University

tltltltlUflClJ VlUl1f1fliJ G1lJ ijru f11f111l11mmjlJBL~nVIjtlilm1u(l~1V1jfllJ1Jlf1lJ f1W~bull li

1mmjlJf11G1~i lJVn1V1C11~C1mlLVIW t1JR1U1J~IllL~tl1nUn1jrrVll1Jlj~UUllJnBL~nVIjtliln~~ LiJ1Jth~1C1ttt1~11~1

tltltltlUflW Vlj f1jf1j fI1~n(l1Jljfl ml1jpoundJth~~lfllf111l1-W1Jofl11G1~i f1ClJ~1V1Ulf11G1~i lJvn1V1Ul~CJ Ln~~j 1l1G1lli vtlViR1LLU ~IllU(l~f111lJ~1lJiitlt 1Jn1jG1~lJ(llCJYllJw~ L~1J LtIvhCJ~LG1lJtI

lJl tltltltlUflWeurolllljaVIjJWj ~Jl1JfIJ f1W~LflilIJf11G1~i lJVn1V1Ul~CJ~U(ljl1l01j ~l1J

LL(l~L-WCJlWCJ1CJ1lJlht Vin1j1~LL(l~1 LfIjl~l11itl(lv11mfl~t1J GC-MS L U1J t1lv1v11CJ~ If _4 CIIIQ 1J CIt cv

tltltltlUflWmlljmL(l~UfI(llm fllf111l11f11mjlJLfllJu(l~~HU1Vl(ltllJ lJVll1V1Ul(lCJ ~~ If I ell I 1 I~

~U(ljl1lO11J VI LVlfl11lJtI4LfIjl~Vl LL(l~1l1mVl(ltl1Jn1j LIJ LfIjtlJ GC-MS L u1JtlUl-3Vl

tltltltlUflClJ1J1CJ~~r41JG B1JVIi-31lJ U~1J1CJ1J~ 11JVI~1lCJ unimilfllf111l1 ~

1mmj1l1vHt1u(l~Eh~nVIjtlilna flClJ~1i11mjllil1~~i lJVl11V1CJ1~CJ~U(ljlIJtnj ~Ii1CJIijVlVll1G1~

~tlmruLL(l~~llIAj~UUllnB L~ nVIjtlilnaL~l1Ijjru

~ilflHm1iv (o1tt11 VlV) mw~lJ1UU~1JnihanVliiln~Li~ij1uunwlJtimLViv1Jv

(o1tt1elmqtt) Development of electronic nose system to classify garlic

type

Lnmm1JLthJmjllVl~nlmltl~I111llJ1lJtILVI PllVIV 1lJLL~rtlijTll1tJrVI11m~ Ln~lJl~

lii1lJ1lJ1J1nvtii n~VfiIJlr1V1 ~lTll1tJrLL~r1lijVlnijVlr1 nVlr1EJ~1EJwlJtivh1l1~u~lofllii1lJ1lJhi jtlEJtI~~U1tyVl11lJm~ L~in~tlTl1ltJaVI11m~Lnm~1m~fl111JtlJm mLViEJ1J (Allium

) ~I - JIII ou tI 1 I r 1Sativum L m ulJelrNaJlVI1~m~ Lnm~1llJVlVllJJVI~Uj Lofln1Jn~ ~~UutyVl1 LlJm~Lrtln~tl lJ

n~LViEJ1Jijtll~tI~ntlUJtl~irL~tlfllJm1J1C1Jv1~~1I1n lV1EJij 5-2 -prope nyl-L -cysteine

sulfuroxide (allin) tl~iiJ 85 ~I~JtJrlJitln~lJLLr~CIJrrnliClJVI1Jj1o1WJtlJnLViEJ1I1V1EJn~1J JtlJmLViEJ1JLOVll1nmv1LilJl~~ allinase 1t1fin~EJ1nU allin 11l1LOVl diallyl thiosulfinate

(allicin) i1J1I1 LLr L~tl~l1nmLViEJ1JLLIJirWlJq~ij~ LLrn~lJv1LL~lnIJi1JnU11l1mLViEJlIU11

ilJqLjlJv1ijEJlIJtl~~IJ~1nfltlEhJ1I1n Bn~J1lJ~Jl~1V1m~lItl1V11~~tI~ntlumjn~L~ tln1i I I t a 1IIIt

mLVI EJlIWlJfiVlJl eHmLVI1lJlJLWifl1Uflll flC1Jo1W LLa~1J~U1JlVlL1JlJLtlna n~CIJLtlW1Jl1Jtll1

~lJfh LLJltlJmL~tln~tlmLVivlIll1JljJnUfl1111~tlJmJtlJ~IJnnflrlJaJ~tlJtl1(EJrhalJalJ~1n ~~1 lV1ElLtlW1tlV1J~lllJt1~~U1JVI~~~1mJVI LJ Jlm~1L~~11l1njLViEJ1J~1nIJi1JtIiLVIPI

Vla1nVla1E1ilJ~~n~JLu11111lJtILVIPllV1EJlii1lJ1lJ1I1n Bn~~aJLnVl1tyVl1mLLtllJa1~VI~tltlrtllltl1J 11 I tIshy

mLVlEllI~1EJWlJtiVILulJVIJltlJmiLLr1fl1~lVl1EJmLVlEJlI~1EJWlJ6V1 LnaLflEJJn1JtlnVl1EJ LVlEJ nJl v bull

LL~1m~lii1 LLlJnilJ~mLViElllrlJ~~1111fIj1 LLlJntltlmt1lJmjllLVlq 1 1~Jl111tl1~m~LnUL~El1V1~tl 1 1 f 3 I tI

l1LLlJnJl11JLLVlrlVl1I1Jtl~ll1Jfi~I~1111~flVI LVI~1nJlJ1V1V1itllJ1V1lJn ~1V1IJmLVlEllI LlJ iLVIfII LVlEJ ~ 1 v

rlJ~LLtiJtltlnl~LjlJ 3 tILnVlJl111tl1~mjLnUL~El1 l~LLri WlJ~LU1 tl1~miLnUL~El1tl~tlJ-d1J 75 - 90 1lJ l~LLri ilJqfll~~Lntt ilJ~nr1Jtl1~m~LnUL~EJ1tl~tlJ-d1J 100 - 120 11J l~LLri ilJ~

1 4 tI G d ~ tll I

U1~U1~ LLrll1J~LUEJJ LVllI I

LLrWlJ~VllJn tl1~miLnIJLnEl1 150 1lJIlJL VlLLn W1JqW1I1LLrWlJ~ bull I ~ cal -II I 1

LUJ~1EJ LLJl~1nm~~UfllJEIJ wWIJJtl~rVlLWEIJWmnEl1nU ~CIJallUJlJtl~~1iiLVlEIJtl~mLVI EJlI VlEI middot 1 a I d J gtAT -J 0 I 1

LLJltlEJ1J nJl111 L 1JlJLtl~EJ1n~1V1W~IJ~u IflVl~~1LLlJnn~LVI EJlIJl1~~1EIWlJqVlllr n~CIJo1EJlJin

Ln~LfliEl~nlJ

~1n~CIJ~lIumlJm~Jli1ll~1~LflijJtll~~niiL~nVlitlijm (Electronic nose or Gas a I I tI tI-- sensor or 0 dor sensor) tI~LnVl~1~nJ1Jl11J1V1fl1111Jl1lJVl1lJJl1EJ L1JlLL itJlJJl111 )mEJ1Lflll

1 4 c cCJ 11 tl J iVl11J~1~nJIJl11J1nUfl1111LJ1IJlJIeuroJI~1iLflllVIL1J1Jtllfl ~n tlIJ L lJm~VlIJli1 ~1V1 N1lEllI

v 4J 0 _

LL1J1fl1111flVlVI~W~lJ1iIJIJ~1LLlJnWlJtim L VI EllIVl1EJmiJli1~nr1JJtlJmLVlEllIJl1~~1E1lllJ6 v bull

~1E1~lJniiLifnVlitlijna L~m~1nLtllJ~UlJ1V1mj-aIJiftllJLVl1J1LLrim~i~lJ1LTitllj11111gnu miLn~Jl~llJtIiLVlPI lV1EJ~uum~rlJlJtln~1n~1mlJnTIlii1LLlJnilJ~niLViEl1JLL~1 aJ~1111~fll-ij

iv

A Q_A

ItLU~U1fj11i1V

t U-11U1ieJilL~tln t -tilJna ~nll1tlilm1lfil~C111~-1~1111t Um1~11in~Utl-1mtLVi eJlJ i II a

~

~ bull tJ ~

41 IVA

~~eJt ~IflJntlLnll1tlUnm1eJ~1 (7 I1U~) lIlJm1~tlUC1Utl-1~tlC111 1tntlUlI1-1LfllJlI~1-1nUlJ1 ~

tJ1tntlU-1tUU~U1j1 ItUU1V1-rU1~n~U1nn1tLVieJlJtQntltlnLLUUt~LjU1tUUtJ~L~tlU~1 fl1UfllJmIlV1Utljtl1mf3tt~~1UL~ULiltliJl1eJ~lJl1l~tl1mf3t Utln1nilU1-1I1~tijmII1~LlfeJjj ~

I I ~ j ~ bull Jamp~ I lot Utlj ~CUVIlJlJLVi tl LVILn~fl11lJfI1~LfltlU LU1t~UlI~1l1~~ C1tylPCU Ln1111i11nLilULiltl1U~t~1l1 LI1l

1iItQnUtJ-1LiJUatyty1CU~~lJItlUt~1mIi~L~UjtUfltllJl1L~tli1~eJt-ti~tJn1ru ADC (Analog to

Digital Conversion) 1iI1mrULlIflilflm1tJ~tlJ1jjatyty1CUUtm~1Lfl~1t~lI1jaa~ (MATLAB)

tOn111lJ1t mUm~VI1flW~m~CUtL1lVi1tlftljjjlJItlUaUtljll1jL~~Tm~1iI1nlJnaL~nll1tlilmllftlj~ ~

mtLVieJlJulJitI1il~ fhflcu~m~JWtm~aUt111lJ1tmUmI~1uUn~UtimtLVieJlJ~1eJLlIflilfl Principal component analysis (PCA) m~~1-11Ulftl-1~tUU1~ ~tUUi~L~Utl1J ut~tUU

tJ~tlJ1jj~jVIlJ~1iItV11m~L11tllJlJitluti~m~~1eJltJ1umlJfltllJl1L~tlivi~WJU1iULtl~UU 1 tJIumlJ LabVIEW ujtlItUUijfl11lJalJPruu~1 t UfUmIlI~tl-1t111~1tl~h~mtLVieJlJt U

tJItLlIf3tll1eJ1n 3 UVIrl~ L~url tleJ1~lflJjtleJ f3t~C1tLn~ tlJ1tJ1~ tllJlI~IJIamp Ut tlu1Uluj iN1ViU ~-1LtJUUVIrl~tJnmtLVieJlJvi1i~tlLaeJ-1 lJ1V11mIL~UtllJC11lItLVIeJ~1eJ~tUU1iIlJn

~ ~ ~ ~

aL~nll1tliln~viai1~1U l~eJ1iItLmElULViElUUt1JI~1atlUtl~fltJ1 tntlU t UaT~ItLVIeJtJtl~ulJit ~1mh~~1 ElLfl~tl-1 GC-MS vi~tl~tJ~jijmIlftlgt1n1f111111mmIlJLflijut~~u1~tltllJ flWt

1f3t1nIllJf3t1alJlimiddot lJVI11l1El1~El~U~1lffj1ii l~ElmciLmElULViElUltltltlUIflIlJ11~ILLmlJL~tltn~1 fl11lJUlJlnIJi1~lftl~a1lri1ulftl~tl~fitJltntlUtUn~Ulftl~mtLVieJlJulJit~Uti Utln1nilutl1L~tlLtJU ml~Uvu-hmtLViEllJ~1tlEi1~vi111lJ1l11lC1tlUaULtJUa1eJ~UtiviUlJlnIJi1gt1nU ~1tlV1-11i1 tQn-u1LtJVI1

tllJ~UfjmIlJ CU n1fl1111~UfjmalJli flCUt1l1El1f3t1alJli lJ1l1El1~ElLn~ImalJli tllJm~ I

1nmI1Lfll1tVIUulJit16mn111lJ1LmElULViElUnUL~tltJItWUml~1uUn~UfimtLViEllJ~1eJ ~

1JnaL~nll~tliln~ utVi1mliWJU1ItUU1~tvLVIlJ1talJnUm~t -tij1U~~lJitlLtJ Utln1nilu~1t U

~1U1iEliltVi1mltn~1V11UU1l11~L~tlll~tl~~wJU1lJnaL~nllltliln~~U 11UUihVl-rU1~a1I ~ ~

ItLVI El1nnItLViEllJiUt -ti-11L1Ltl~8nJI1El

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 4: 21 .11. 2556 - Ubon Ratchathani University

~ilflHm1iv (o1tt11 VlV) mw~lJ1UU~1JnihanVliiln~Li~ij1uunwlJtimLViv1Jv

(o1tt1elmqtt) Development of electronic nose system to classify garlic

type

Lnmm1JLthJmjllVl~nlmltl~I111llJ1lJtILVI PllVIV 1lJLL~rtlijTll1tJrVI11m~ Ln~lJl~

lii1lJ1lJ1J1nvtii n~VfiIJlr1V1 ~lTll1tJrLL~r1lijVlnijVlr1 nVlr1EJ~1EJwlJtivh1l1~u~lofllii1lJ1lJhi jtlEJtI~~U1tyVl11lJm~ L~in~tlTl1ltJaVI11m~Lnm~1m~fl111JtlJm mLViEJ1J (Allium

) ~I - JIII ou tI 1 I r 1Sativum L m ulJelrNaJlVI1~m~ Lnm~1llJVlVllJJVI~Uj Lofln1Jn~ ~~UutyVl1 LlJm~Lrtln~tl lJ

n~LViEJ1Jijtll~tI~ntlUJtl~irL~tlfllJm1J1C1Jv1~~1I1n lV1EJij 5-2 -prope nyl-L -cysteine

sulfuroxide (allin) tl~iiJ 85 ~I~JtJrlJitln~lJLLr~CIJrrnliClJVI1Jj1o1WJtlJnLViEJ1I1V1EJn~1J JtlJmLViEJ1JLOVll1nmv1LilJl~~ allinase 1t1fin~EJ1nU allin 11l1LOVl diallyl thiosulfinate

(allicin) i1J1I1 LLr L~tl~l1nmLViEJ1JLLIJirWlJq~ij~ LLrn~lJv1LL~lnIJi1JnU11l1mLViEJlIU11

ilJqLjlJv1ijEJlIJtl~~IJ~1nfltlEhJ1I1n Bn~J1lJ~Jl~1V1m~lItl1V11~~tI~ntlumjn~L~ tln1i I I t a 1IIIt

mLVI EJlIWlJfiVlJl eHmLVI1lJlJLWifl1Uflll flC1Jo1W LLa~1J~U1JlVlL1JlJLtlna n~CIJLtlW1Jl1Jtll1

~lJfh LLJltlJmL~tln~tlmLVivlIll1JljJnUfl1111~tlJmJtlJ~IJnnflrlJaJ~tlJtl1(EJrhalJalJ~1n ~~1 lV1ElLtlW1tlV1J~lllJt1~~U1JVI~~~1mJVI LJ Jlm~1L~~11l1njLViEJ1J~1nIJi1JtIiLVIPI

Vla1nVla1E1ilJ~~n~JLu11111lJtILVIPllV1EJlii1lJ1lJ1I1n Bn~~aJLnVl1tyVl1mLLtllJa1~VI~tltlrtllltl1J 11 I tIshy

mLVlEllI~1EJWlJtiVILulJVIJltlJmiLLr1fl1~lVl1EJmLVlEJlI~1EJWlJ6V1 LnaLflEJJn1JtlnVl1EJ LVlEJ nJl v bull

LL~1m~lii1 LLlJnilJ~mLViElllrlJ~~1111fIj1 LLlJntltlmt1lJmjllLVlq 1 1~Jl111tl1~m~LnUL~El1V1~tl 1 1 f 3 I tI

l1LLlJnJl11JLLVlrlVl1I1Jtl~ll1Jfi~I~1111~flVI LVI~1nJlJ1V1V1itllJ1V1lJn ~1V1IJmLVlEllI LlJ iLVIfII LVlEJ ~ 1 v

rlJ~LLtiJtltlnl~LjlJ 3 tILnVlJl111tl1~mjLnUL~El1 l~LLri WlJ~LU1 tl1~miLnUL~El1tl~tlJ-d1J 75 - 90 1lJ l~LLri ilJqfll~~Lntt ilJ~nr1Jtl1~m~LnUL~EJ1tl~tlJ-d1J 100 - 120 11J l~LLri ilJ~

1 4 tI G d ~ tll I

U1~U1~ LLrll1J~LUEJJ LVllI I

LLrWlJ~VllJn tl1~miLnIJLnEl1 150 1lJIlJL VlLLn W1JqW1I1LLrWlJ~ bull I ~ cal -II I 1

LUJ~1EJ LLJl~1nm~~UfllJEIJ wWIJJtl~rVlLWEIJWmnEl1nU ~CIJallUJlJtl~~1iiLVlEIJtl~mLVI EJlI VlEI middot 1 a I d J gtAT -J 0 I 1

LLJltlEJ1J nJl111 L 1JlJLtl~EJ1n~1V1W~IJ~u IflVl~~1LLlJnn~LVI EJlIJl1~~1EIWlJqVlllr n~CIJo1EJlJin

Ln~LfliEl~nlJ

~1n~CIJ~lIumlJm~Jli1ll~1~LflijJtll~~niiL~nVlitlijm (Electronic nose or Gas a I I tI tI-- sensor or 0 dor sensor) tI~LnVl~1~nJ1Jl11J1V1fl1111Jl1lJVl1lJJl1EJ L1JlLL itJlJJl111 )mEJ1Lflll

1 4 c cCJ 11 tl J iVl11J~1~nJIJl11J1nUfl1111LJ1IJlJIeuroJI~1iLflllVIL1J1Jtllfl ~n tlIJ L lJm~VlIJli1 ~1V1 N1lEllI

v 4J 0 _

LL1J1fl1111flVlVI~W~lJ1iIJIJ~1LLlJnWlJtim L VI EllIVl1EJmiJli1~nr1JJtlJmLVlEllIJl1~~1E1lllJ6 v bull

~1E1~lJniiLifnVlitlijna L~m~1nLtllJ~UlJ1V1mj-aIJiftllJLVl1J1LLrim~i~lJ1LTitllj11111gnu miLn~Jl~llJtIiLVlPI lV1EJ~uum~rlJlJtln~1n~1mlJnTIlii1LLlJnilJ~niLViEl1JLL~1 aJ~1111~fll-ij

iv

A Q_A

ItLU~U1fj11i1V

t U-11U1ieJilL~tln t -tilJna ~nll1tlilm1lfil~C111~-1~1111t Um1~11in~Utl-1mtLVi eJlJ i II a

~

~ bull tJ ~

41 IVA

~~eJt ~IflJntlLnll1tlUnm1eJ~1 (7 I1U~) lIlJm1~tlUC1Utl-1~tlC111 1tntlUlI1-1LfllJlI~1-1nUlJ1 ~

tJ1tntlU-1tUU~U1j1 ItUU1V1-rU1~n~U1nn1tLVieJlJtQntltlnLLUUt~LjU1tUUtJ~L~tlU~1 fl1UfllJmIlV1Utljtl1mf3tt~~1UL~ULiltliJl1eJ~lJl1l~tl1mf3t Utln1nilU1-1I1~tijmII1~LlfeJjj ~

I I ~ j ~ bull Jamp~ I lot Utlj ~CUVIlJlJLVi tl LVILn~fl11lJfI1~LfltlU LU1t~UlI~1l1~~ C1tylPCU Ln1111i11nLilULiltl1U~t~1l1 LI1l

1iItQnUtJ-1LiJUatyty1CU~~lJItlUt~1mIi~L~UjtUfltllJl1L~tli1~eJt-ti~tJn1ru ADC (Analog to

Digital Conversion) 1iI1mrULlIflilflm1tJ~tlJ1jjatyty1CUUtm~1Lfl~1t~lI1jaa~ (MATLAB)

tOn111lJ1t mUm~VI1flW~m~CUtL1lVi1tlftljjjlJItlUaUtljll1jL~~Tm~1iI1nlJnaL~nll1tlilmllftlj~ ~

mtLVieJlJulJitI1il~ fhflcu~m~JWtm~aUt111lJ1tmUmI~1uUn~UtimtLVieJlJ~1eJLlIflilfl Principal component analysis (PCA) m~~1-11Ulftl-1~tUU1~ ~tUUi~L~Utl1J ut~tUU

tJ~tlJ1jj~jVIlJ~1iItV11m~L11tllJlJitluti~m~~1eJltJ1umlJfltllJl1L~tlivi~WJU1iULtl~UU 1 tJIumlJ LabVIEW ujtlItUUijfl11lJalJPruu~1 t UfUmIlI~tl-1t111~1tl~h~mtLVieJlJt U

tJItLlIf3tll1eJ1n 3 UVIrl~ L~url tleJ1~lflJjtleJ f3t~C1tLn~ tlJ1tJ1~ tllJlI~IJIamp Ut tlu1Uluj iN1ViU ~-1LtJUUVIrl~tJnmtLVieJlJvi1i~tlLaeJ-1 lJ1V11mIL~UtllJC11lItLVIeJ~1eJ~tUU1iIlJn

~ ~ ~ ~

aL~nll1tliln~viai1~1U l~eJ1iItLmElULViElUUt1JI~1atlUtl~fltJ1 tntlU t UaT~ItLVIeJtJtl~ulJit ~1mh~~1 ElLfl~tl-1 GC-MS vi~tl~tJ~jijmIlftlgt1n1f111111mmIlJLflijut~~u1~tltllJ flWt

1f3t1nIllJf3t1alJlimiddot lJVI11l1El1~El~U~1lffj1ii l~ElmciLmElULViElUltltltlUIflIlJ11~ILLmlJL~tltn~1 fl11lJUlJlnIJi1~lftl~a1lri1ulftl~tl~fitJltntlUtUn~Ulftl~mtLVieJlJulJit~Uti Utln1nilutl1L~tlLtJU ml~Uvu-hmtLViEllJ~1tlEi1~vi111lJ1l11lC1tlUaULtJUa1eJ~UtiviUlJlnIJi1gt1nU ~1tlV1-11i1 tQn-u1LtJVI1

tllJ~UfjmIlJ CU n1fl1111~UfjmalJli flCUt1l1El1f3t1alJli lJ1l1El1~ElLn~ImalJli tllJm~ I

1nmI1Lfll1tVIUulJit16mn111lJ1LmElULViElUnUL~tltJItWUml~1uUn~UfimtLViEllJ~1eJ ~

1JnaL~nll~tliln~ utVi1mliWJU1ItUU1~tvLVIlJ1talJnUm~t -tij1U~~lJitlLtJ Utln1nilu~1t U

~1U1iEliltVi1mltn~1V11UU1l11~L~tlll~tl~~wJU1lJnaL~nllltliln~~U 11UUihVl-rU1~a1I ~ ~

ItLVI El1nnItLViEllJiUt -ti-11L1Ltl~8nJI1El

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 5: 21 .11. 2556 - Ubon Ratchathani University

iv

A Q_A

ItLU~U1fj11i1V

t U-11U1ieJilL~tln t -tilJna ~nll1tlilm1lfil~C111~-1~1111t Um1~11in~Utl-1mtLVi eJlJ i II a

~

~ bull tJ ~

41 IVA

~~eJt ~IflJntlLnll1tlUnm1eJ~1 (7 I1U~) lIlJm1~tlUC1Utl-1~tlC111 1tntlUlI1-1LfllJlI~1-1nUlJ1 ~

tJ1tntlU-1tUU~U1j1 ItUU1V1-rU1~n~U1nn1tLVieJlJtQntltlnLLUUt~LjU1tUUtJ~L~tlU~1 fl1UfllJmIlV1Utljtl1mf3tt~~1UL~ULiltliJl1eJ~lJl1l~tl1mf3t Utln1nilU1-1I1~tijmII1~LlfeJjj ~

I I ~ j ~ bull Jamp~ I lot Utlj ~CUVIlJlJLVi tl LVILn~fl11lJfI1~LfltlU LU1t~UlI~1l1~~ C1tylPCU Ln1111i11nLilULiltl1U~t~1l1 LI1l

1iItQnUtJ-1LiJUatyty1CU~~lJItlUt~1mIi~L~UjtUfltllJl1L~tli1~eJt-ti~tJn1ru ADC (Analog to

Digital Conversion) 1iI1mrULlIflilflm1tJ~tlJ1jjatyty1CUUtm~1Lfl~1t~lI1jaa~ (MATLAB)

tOn111lJ1t mUm~VI1flW~m~CUtL1lVi1tlftljjjlJItlUaUtljll1jL~~Tm~1iI1nlJnaL~nll1tlilmllftlj~ ~

mtLVieJlJulJitI1il~ fhflcu~m~JWtm~aUt111lJ1tmUmI~1uUn~UtimtLVieJlJ~1eJLlIflilfl Principal component analysis (PCA) m~~1-11Ulftl-1~tUU1~ ~tUUi~L~Utl1J ut~tUU

tJ~tlJ1jj~jVIlJ~1iItV11m~L11tllJlJitluti~m~~1eJltJ1umlJfltllJl1L~tlivi~WJU1iULtl~UU 1 tJIumlJ LabVIEW ujtlItUUijfl11lJalJPruu~1 t UfUmIlI~tl-1t111~1tl~h~mtLVieJlJt U

tJItLlIf3tll1eJ1n 3 UVIrl~ L~url tleJ1~lflJjtleJ f3t~C1tLn~ tlJ1tJ1~ tllJlI~IJIamp Ut tlu1Uluj iN1ViU ~-1LtJUUVIrl~tJnmtLVieJlJvi1i~tlLaeJ-1 lJ1V11mIL~UtllJC11lItLVIeJ~1eJ~tUU1iIlJn

~ ~ ~ ~

aL~nll1tliln~viai1~1U l~eJ1iItLmElULViElUUt1JI~1atlUtl~fltJ1 tntlU t UaT~ItLVIeJtJtl~ulJit ~1mh~~1 ElLfl~tl-1 GC-MS vi~tl~tJ~jijmIlftlgt1n1f111111mmIlJLflijut~~u1~tltllJ flWt

1f3t1nIllJf3t1alJlimiddot lJVI11l1El1~El~U~1lffj1ii l~ElmciLmElULViElUltltltlUIflIlJ11~ILLmlJL~tltn~1 fl11lJUlJlnIJi1~lftl~a1lri1ulftl~tl~fitJltntlUtUn~Ulftl~mtLVieJlJulJit~Uti Utln1nilutl1L~tlLtJU ml~Uvu-hmtLViEllJ~1tlEi1~vi111lJ1l11lC1tlUaULtJUa1eJ~UtiviUlJlnIJi1gt1nU ~1tlV1-11i1 tQn-u1LtJVI1

tllJ~UfjmIlJ CU n1fl1111~UfjmalJli flCUt1l1El1f3t1alJli lJ1l1El1~ElLn~ImalJli tllJm~ I

1nmI1Lfll1tVIUulJit16mn111lJ1LmElULViElUnUL~tltJItWUml~1uUn~UfimtLViEllJ~1eJ ~

1JnaL~nll~tliln~ utVi1mliWJU1ItUU1~tvLVIlJ1talJnUm~t -tij1U~~lJitlLtJ Utln1nilu~1t U

~1U1iEliltVi1mltn~1V11UU1l11~L~tlll~tl~~wJU1lJnaL~nllltliln~~U 11UUihVl-rU1~a1I ~ ~

ItLVI El1nnItLViEllJiUt -ti-11L1Ltl~8nJI1El

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 6: 21 .11. 2556 - Ubon Ratchathani University

v

~EJL1Gl1 12 Lfis1J (UJli1EJ1J 2554 -

nlilniill 2555) ~-~--r-----r---r--r-----~

1 n1iL(fl~V1J~11J1ivVlUVl11JAntj1

LeuroIni1Tl

~11J1~EJjlI1La1JSn1l~1LL1Jn~1J~n~LViEJ1J~1EJlillJnaL~nVliS-untf 1flEJvhn1~Ei1Ja1JfI111J LLflnlJh~lJs~ti1JGmLiljv1Jvlu11J11il1n 3 LLfl~ 1~LLti SV1~d1JjSEJ lilfII~aLnli sJ1lhfl Iil SfIl~fltl LLGl Sj11J100 1il~11J ~1EJn1ia~1~Gl1vi1Jl1fiLa1JLss1YllGl~L1J~nCiioa11J1l(lEi1Ja1J1~

bull II

11mLViEJ1JvIj11J1V1flasULj1JmLViEJ1J~hodijfln1J LLGl1il1nn1i1sofhhnsUVl1oLfljlJson~1J mLViv1J1il1n1sss1J1fli1J11f1m1~viL~1il1mVIflijfl GC-MS U11mLViv1JLLGl~1JGijilflh1JlJs~ sofl1hnsUt1Ja1iiLvjfl111JLLfln~hot11J 1flEJIil1mJGln1~VIflGlso~oa~HLVIfl-UflLLaflotxL~1J mLViEJ2J~11JLLGlSfIl~fIampijfl111Jtmliflt11J1J1nn11nlLViEJ1JfII~aLnli LLGl S11ilnfl111~11

II bull

n iLViV1J1il1n LLflotl~nvl10n1Jlilijfl~11JlJSoSofltlinSUVI1oLflijvlLLfi nfi1~n1Ja Sflflfl SonU

a1J1J~11J1~~oH CiioLLaflotxL~1J~jfl111JLj1J1tllm1Jn1i~1LL1Jnti1JqmLVivlI~1VlillJn aL~nVI~sijn~ iUU1fln~1JmLViv1J~1VIil1JnaL~nVIisijn~Sn1dLd1JLdsivla~1~1Jr1JtlinsU

II do ~

1tl~1EJn1dLd1J LdSivlijfl111J11~Sa1i~1jt11J~o1Jfl 7 m LLGlUJSVI1n1iVIflGlSJ1flnGl1JlISJ

mLViEJ1JU11n1t1Ld1JLdsi~flSUa1JSJSa1lt1Jmi1JLLSGlnSOSaliljfl1111bt1Jn1lflsUa1JtlJtl n~1JlImmLViEJ1JW~Jrn t1JlJruvln1t1Lyj1JLtItli~fltlUa1JsJfitlLLS1J11lLilVLLGllTfiL1Jijfl111Jbfitl n~1JniLViv1J~Jn11n1t1Ld1JL1midijfl~1J 1 LLGlLi1tlj111tllJGlm~1flmLViEJ1 3 d-Ufl 1 Gl 12 fl~J 1J11Lfl~1l1~1LL1Jnti1JtfLflEJLVIflijfl PCA nU11itllJGllJtlJ~1sv1JmLViEJ1dijflLfiV1t11JjLL1J11j1

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 7: 21 .11. 2556 - Ubon Ratchathani University

bull bull

vi

m~~1V~18~bJh~fh8~Ilh~n8JanmnaLfiv~nU il~wi~~WJm~~8UnU~~d1~n~1Ij1~LL~n L~ULLJ1Lj1lJ8~LLIJi~~n~lIiUfim~LVimMi8Eh~~~L~U ampJ~m~Vl~~8~duUcJUf)1111G11111~Cl1Um~ j1LLUniUfim~LVivlI~1nn~UJ8~m~LVivlI~1m~JJ~lInaLanV1~8ijn~~LtJU~~tJtJ~~1VLL~~ij fh1ij~1V~1L111~~~~iJlJU1LtJULfl~8~iJ81UmlL~lIlJ~fh~UA1Lnlffi~~81J

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 8: 21 .11. 2556 - Ubon Ratchathani University

UVIAflUB i

Abstract ii

UVIaltl~U~II1l iii d 0

UVIVI 1 UVIU1 1

11 fl11lJ s OJ ft1 OJ01f1tyUGI~VIlJ1l1tlJlJtyll1V1V11ml1~lI 1

ltOJ tI ~ ~ ltOJ12 1t9ll laJfIlItlJ fIlJn1l1~lI 1

13 lItlULtJIJItJBJ1RlJn1l1~tl 1 o oJ t ~ tl4LI

14 LLt-Jumlfl1L UUJ1Ut9lGltlfl ~RlJml1~lIuGI t-JGIJ1UVIR1f111l bfllU 2

i ~ 1bull15 t1 lLtlllUVIR1f111~ f1ltJ 3 d JJ

UVIVI 2 VIqlef~VILnll1t1i)4 4

21 nlLVitllJ 4 -~ ~( )22 ~nBLGlnVIltlUna Gas Sensor 5

23 Gas Chromatography-Mass Spectrometry (GC-MS) 6 a G

24 n1iai1JGl1l1VlJVf1LBULB6

251t1iLLnilJ LabVIEW ~ 8

26 Principal Component Analysis (PCA) 8 ~1t1 ~ II

UtItI 3 n1a1Lft1Ki)~ anaUt114Lftll Ufl1aaL~UJLLfttIaftWUqnaa1ltla4naLt11J1I 9 cl shy

31 n1SLntJIJI1BlI1jna~LVllIlJ 9 4IItI ~_ d

32 miai1JGl1UVlJVf1LBULBtIBJmLVIlIlJ 10 321 i1UIJIBUn1iVIflGlBJ 1 0

322 oJGln1lVIflGlBJ 13

323 1~1iwt-JGln1lVIflGltlJ 14

33 ma1LRa1~BJfltlinBtJtUa1iiLtl 14 331 i1Ut9ltlUn1aVIflGltlJ 15

332 t-JGln1aVIflGlBJ 15

323 11iW~Gln1lVIflGlBJ 19 41 tI CII

UVIVI 4 iUU1f1nftULLft1LLUflWUqnaLt1all21 0

q~ q~ bull

41 lUtJ1f1f11tlnBLGlnVIiBUn~ 21

42 ~ n1aVIflGltlJ1f1nGlUni~LVIlIlJ 23 421 i1U9ItlUn1lVlflGlElJ 23

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 9: 21 .11. 2556 - Ubon Ratchathani University

422 ~Gln1iVWIGleJ~ 25

43 n1~i~fIi1~~1~1Ini1l~mLVimJ~W~Vlflijfl PCA 29

J tI IIUVlV1 5 ~1 u~tIeJ~~1JeJU1J 31

LeJn~1ieuroh~E~ 33

n91~n1It1in1f1 35

n1f1~1J1n n 36

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 10: 21 .11. 2556 - Ubon Ratchathani University

d UVIVI 1

o UVIU1

Lm~tlimi1JLiJUel1iiWVInlJBltJi~lI1l1U1UtJi~LVlllVlEJ 1ULLvifl~tJiJyjll(JflVl1lmiLnlli

~1U1UlJ1n~ El El m1Elltlfl1Vl ~lyjll(JmLlPifl~lIijVlniJVIfl1nVIfl1 EJ~1EJ~U5vh1~tU~Lnfl~1U1U111

JElEJtJi~~utlqJVI11UmiL~ Eln~ElYh1(JflVl1lmiLnlll1m~f1111J~Ellmi nl~LViU1JfiLiJU(Jfl(J~~Vl1l mlLnllil1iiVlVIUlvitu~LnflniTn~~tJit~utlfllVI11U mn~Eln~El LVlU L1lW1~Elthl flllUtl~~uU~ VIt~1miJVlLllVlmifl1 L~ivh1~m~LViU1J~1 nvi1ttJl~LVlIVIfl1 nVIfl1u~Uq1natL-U1lJ11UtJl~LVII

lV1EJ~1U1U1J1n mlL~Eln~Elm~LViu1Jl~tlllnUfl11lJVfEllmillEll~U~Lnfl~lLtJUmlEl1P1tJ1h~UiJU ~1n~f11 ~nvfliJlLnVltlqfVI1miLLElUe11lVI~EltJflEllJtJUm~LViu1J~1UUqviLtJU~VfEllmiufl~l1f111J 1um~LViulJ~1uUqmnaLiulnU~mIl1u LVlutJnjiua1mi~1LLUmUqmLViUlJjU~~~1UUn~1lJ

I dO 0 bull 1 J 0 ~II IEl1Eln1i Lnu LmJ1V1l El~1 LLUn1Jl1lJUVI fllVl1J1llElJWU~ lIlVI1ntl1VlVlnd~fI11lJ1U1qfmuULlElJU1nVl

~U~Lnflil~~1lJ1ll~1UUnfl111JU~nvi1J ~ I II - 4IIIocJ tI 0 tI ~ q II

~1nVlnfl11lJ1ll1J1JlU ~1~UlJUU1f111lJflVlVlil~WfAJU1l~UU~1uUnWUQm~LVlUlJVl1Un1l

1Jll1~fn~UllElJm~LViEJ1Jvi1J~1EJUq1U~lJna~nVllElijn15 (Electronic nose or Gas sensor or

Odor sensor) L~Ell~1miJUi~uu1Vl~iu~ElULVIlJ1~LLtin1iJlJU1L~Elj1lJ11nun1iLn~l1U tJi LVII LVlEJi~UUmjUUEln~1n~1nUn1i~1LLUnwUqm~LVi EJlJ LLpoundl1 iJla1~1il1-mUn1lind1

- Ii I 1 I J f1W~lJU~llEllm~LVlU1JIIUVlIJl1l LWElL1JUllEllJaLUn1iLW1~ uflnLLa~LnULnU1IoVlElnVl1tVIUtmu I

12 1ltl5i1~Alla~lf1middotHn151~1J

1) L~ainnflWalJtmllEll~lJniiL~nVllElln~~iJviEln~UllElln5~LViUlJa1uUqvi1l 1U

d II 0 tf

2) LWEl~l1liUUil1LLUnWUQni~LVlUlJ

3) L~ElVI1 LLU1V11l~~1la1V1~UfAJU1illJnaL~nVliElijn15ffULEll

13 lIaULlIlIa~lflJ~n1J1~1J

1) ~1lJ1ll~~IUUnni~LViEJlJ~1UUfiLU1 a1uUHnfl1l ua~a1EJUfiVlunmVluliil1Jn ~ -= ftltQ

ElLflnVliElUn~

2) ~luua1V1~U91JU1~1JniiL~nVllEliinamp1

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 11: 21 .11. 2556 - Ubon Ratchathani University

2

1 n1l~PlEJlH1U1~tlYlUYl1U

fim1Lilna$

2 f11V1UflurIUn1ln5n1l

fi1Lilu~1U1~tI

32 1111

34 ~nJlIil2jfl DNA

421LfIl1l~iiilllflnn GC UfIl U

iiil2jfl DNA

43 LtRmJLVitluiiillJfInnifil

32 flU 33

~fI~1U~llftl1n nlnllll

1)illlU1hm~UulVI

UfI~ u~Un1WlflfI euroI]

l~UU1I11n u

niaVi tlll)1 tI llJ n -shyilLflnYliilUnH((

1)iftlllfl ll~1I1MI1l

llL1tIlum~Liitlll

1) if tI 2j fI DNAlI tI ~

mLVltllI

1)flcu~nCU~Yl1UfHI

~tyty1cu1YIh~11tl1) bull 0

Iil1nnflUnlaYltlll

~ fl L ll1 tI U L VI V U

1 ~ 111 euroI2jfl DNA

UfIm1l1MI1llL~tI

o iLl ((

Iil1UUnWUlini~LYltlll 1111t1tiilillln

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 12: 21 .11. 2556 - Ubon Ratchathani University

3

15 t1jlvtlj~fl1flhlJiiu 151 tIGltl~fl

- UVIfl111lvirl1vrh~~vhJn 1I11LG1UtJ L~tJi Development of electronic nose

system to classify Thai garlic type fl1fl11~ai~~llnltVi International Journal of

Food Science and Technology VI~tJ IEEE Sensors Journal VI~tJ Sensors and

Actuators Journal LUU]U

- tIGl~1UL~tJ~flVIiWti~UVI1~1tyty1 SJu1fln~ULLGlij1LLU nU~mLViVll~1V~lJn A ~ 4

tJLGlnVI~tJUnG1

152 tI~~W5 w bull

~1U1uVilijfl111l1i~Vl1~11~~G111l1S~bvL~lltl~~VI6fl1W1Ufl1S)1 LLU nU 5 mL ViV1l8 U ~LtJUVIUVI1~Vlii~vi~lbvL~1l1lGl fl1IJ tJ~~1nfl1~ufl)1V1hv tI ~tI ~flVI1~fl1~Lnllm1~ U tJ n~1ndiJ~

LuUfl1~1iIJU1V11~~1ULVIflLUlGlVVI1~fl1~Lm~W1~~li1fJ1~Il1f1tJflG11V1mS1lG111l1~~I111tlIi~1U1~~~~

III I r1 ltI 1

lilT 11 11 l~

UGlGlflfll LlJ~1EHUfl1StI8~ umruS1f11UftJnnfl1J uS~LVlfl fl ~flV~UUVl flUUUtJn~1n ~lJ LU

fl1Sij1LLUnU5mLViVllLL~1 iTJG111l1~~1m Ufl1~jnll1f1ruG11lmIJtJJmLViVlllJijfl9i1~ L~m uU bull I v I 1 I lv I IIlJ81lGlLUfl1SLW1 uGlnLLGlLnULnfn fltJnVl1JVIU~fl1V

_ r Iv 1 Iv l II I II 0 Irtffl mL VlVllVl LtI LUJ1U1~VUoil~ LlJm~LVIVllVIVl1Lflll1V LUVl8J~U LU8Joil1nflt)m1~oil1nflfl1

uJ~~UviIl1Uflll1mm~1l1nvi1fl mill~18a1Jm~~~8~1l1oil1nuVlaJJ~flL~v1nU ~JtU1Ufl1~ fi1LijUfl1~1uV~~~t)~11lijt)nuLn~~mL~t)1m~n~1J~1t)a1~viijLi8141lJfl11J~t)Jn1S JGlfl1~1~V ~~nl111t1JiiJLViviJniJIl111Jli1U1tylJ8~Ln~~mLTJtJ1hlJ~m~1i~114oil~~~81t1

UiJ1fln~Um~tUiTJG111l1~t1S~nmmUmij1uUnVl~8JUjUtI~flJGlVI1~n1Ln~~ ~U ~lJi~n W11oilLUUVltJllUfli VI~t) Wnq~ru1 lGll UGliTJJ~~nfl1mUmt1LijUfl111J1n lJt)~JGl1m~~n~1v

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 13: 21 .11. 2556 - Ubon Ratchathani University

d UVIVI 2

21 mavmJ

fliLVimJ ii~eljVltJ1F111~il1 Allium Sativum L LtlU~lIa1lflmhLflVlentJtiU 1IUl1~ ~~nmaVCJ1IUfl 111111tillUmilh~nelUenVlT~Uflin~n1itUJ1LtJULm 1l1u~UtJLLaJ 1UmLvtJ1I1

iiel~lhh~nelulJel~~flLvlelTIUtJ~1I1U1~I~1I1n 1~lCJ1Ul1ii~LVltJlJel~m~LVi CJ1ItJinelU1tJ~1 tJ 1

l1itJ)~nelUVl~flIJel~ hydrogen sulphide methyl sulphide dimethyl sulphide LLfl allyl

sulphide [1] 1lltJii S-2-propenyl-L-cysteine sulfuroxide (allin) el~ih 85 ~~~~CJllPIeln~U LLl~flUl~miUl~Vl1~gll11~lJel~m~LViCJ1I 1lltJn~UlJel~m~LViV1ILnIl1iJ1nmi~LelU1ij21 allinase vi1 tJijn~V1nu allinvi111XLnll diallyl thiosulfinate (allicin) JU1I1 tJ~ffiV1IJel~el~fltJinelULflii

1Um~LVivlIUiifl1111~U~elU1I1flLLl1~iimiPim~nnLIel~1~LL~~Vlfl1tJ ~~LliLI Yu at eL [2]1~ vi1miPifl~nel~fltJinelulJel~a1iLflmLIl1ii~LVltJlJel~mLVivlI~1v GC ~uhmianlla1iiLVlV

~1vy1LIvh11Xbiillmi~YL~V~1L1tJinelUU1~el~1~1tJ Amagase at el [3 4]1~PimY1aii~fJru I bull

llel~maVlVlIVIlICJl~elaIJfl1~ 1LImiPinlnLmvuLVitJumLVitJ1I1iJ1fltJibVlfllIPl1~ ~tJel~fltJi~nelU1LIl1iiLVltJ1lltJ115

Gas chromatography with flame photometric detection (GC-FPD) ~U11a1iibVltJtlel~

mLVi pound111 OJ 1nLLIPIl~UVl~~iiel~fltJinelUK~nLKijelUnU UIPI ~iifl1111UlJ1nIPl1~tUm1l1rua1iiLVl tJU1~ lfiJIl [5] LIelfll1niTILImiPinll1LmtJULVitJUel~fltJinelUlJel~a1iiLVlViK11~mLVitJlIViiiel1V 1P11~ nLI LPiV GC-FID bLl GC-MS ~U11niLVitJlIeielUiim1l1ruIJel~ dially disulfide diallyl

trisulfide allyl methyl disulfide bLl allyl methyl trisulfide 1I1fln11mLViVlIm~L~1I~LLa1 [6]

UelflIiJ1fllua1cj~iimiPiflll1b~V1nUmibnPln~U~PltJ1flvtw~~tJiI~flVl~~miU~Lflfl mLViv1Iel~11fl11~lJ11~ Tamaki at el 1flPinll1LmVULVivun~UlJel~mLViv1IaPlbLfl~mLVivlI~ tJi~anLLa1Vl~miu~1flfl1IlmmvubVivuCJmi1PlIiJ1flliJlIn8baflVIieliJm1nuCJlm~OJ1nII Gasbull

chromatography ~~~u11mLVivlI~tJl~1flualOJiifl~UlUUi~tl1jelVl~ [1]

Gt1KiumbViV111UtJiLVlfll1V1vUOJuu~eleln1vlbUU 3 tJ)LfIVI1J1111el1VmiL~uLriV1bull 1flLLti ~UfiLU1 envmiL~UL~V1elv1L1li1~ 75 - 90 1U 1~uti ~Ufifll~aLnll ~LIfiflm~el1VnTn~uU LfiVTtlV1U-d1~ 100 - 120 1U 1flUti ~LIfiU1~-H1~ bm~UfiLgv~lK~ LLl~UfiVlrfl el1tJmib~U LnV1 150 1UiiLI1tJ 1flLLti ~LIfi~~1LLfliUfiLwa1V bLIPl1iJ1nmi~uAUcj~1~~urel1llViL~V~~el

1

Lrimnul UIl1IUiiIJel~a1iiLVlVlJel~mLVitJ1I1VlV

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 14: 21 .11. 2556 - Ubon Ratchathani University

5

22 1iJ~n~LanVIivUnt1 (Gas Sensor) 1ijlJnBLanVl1vum1LtJU Chemical sensor ~jijVI1fljjUVl~1fJfiU LVlflLL~~jjuVl1ij~ij

VIan mi111j1ULb~m1btl ~ClUbbtlj1leuroJjtfhJ1ruVl1jYlam1V1~euroJLf1Iij~LLJlnlgthjnUeuroJeuroJ n 1 tl Cln~1euroJthj

Ll1U 11UVl QCM mrVIanfl1i1leuroJ~ Mass sensor ~ijf1l11lJ51leuroJlmiiU1leuroJ~f-lanf1l1euroJVli (Quartz)

Ltl~ flULLtl~Jl1lJmlJ1ru611iLf1Iij~~n~wlrUUUti11leuroJ~f1I1euroJVli [7 -9] VI~euroJ11UVl611in~1gtl1th (sem icond uctor type) 1ij~ijf1l11lJ~1UVl1Ufl1Cl1ULLtli ~UJl1lJtlfin~mLf1Iiji~VImiddoth ~ 611i~jIgtlTlhnU f1I11lJbiflJifU1leuroJI~1iLf1Iij~btJUeljfitli~nelUtUfhf(l~Jli11ij1Vl btJU~U [10-13]

LVl fltU~Q1ij~mh1 lbu~1~VIan n1i11111U1leuroJ~1ijlJnBba nVlielilnalilVl ~1 i ~~1gtl1t11~jii

f1I11lJ11t Un1i1Vlal bbJlbJrielfl1~-sU~mmV1Uil1nmiLtl~flUbLtlI1leuroJ~61f111~bb1VlfteuroJlJ G11V1~U161Vl Jli11ij~ (Sen sino material) tYUil~LtJUa1jLVI~eleuroJn1f(1ii LVlflvT11tlRtl Sn02 (Tin oxide) ~ujeuroJon ~ vh1l1~tlU~JIeuroJruVIflihi1LYn~ri1V1j~ euroJeuroJn~LilUil~OnVlVliu (adsorb) uUti11ltll~aneuroJeuroJn1l1Vl1~

~1fl1U il1ntYUBLanJlieuroJUml1 (Donor Electron) 1Uti1f-liiniJ~onri1flLtlU1tlfiltleuroJn~LilU~onVlVl

v V

eBU11 Gil~111LVIaeleuroJ4f11f11tli~~U1nt UuUvihl1ltlltli~~ tlmiddotnn)n1iwQ111tl1LnVlPimfl~vh I I I 11 i 01 I bullbull(1 1It Inil1lVl1l11~ blJ VI~1V1~Lf1ItlUVlelmlel61i1Jl1lJi uVl 21 bVlflf1l111lJl1UVl1U bn1l11lell1Jl1Jli1iliil1lUeuroJfl

tiuri1Pimfl~Tl1d 1lru~n1f(1LihVI1l1flijtl~1l1rulJ1 n~U f1I111lVIJ1 LLllUtJeuroJIeuroJ4f11 f1Itli~~U1lell euroJeuroJnL1iJUil~VlIGil~atl1PimiL~vhU~L1rujelEJIJ1euroJiiri1Vlal~1fl lJ111lalln1ivi 21 LLitlvi 22

Grain boundary Elec1ronbull Electron

ltlvi 22LlJLVl1ltllPinmliTl1u~L1ruieuroJfllJ1euroJltlvi 21 LlJLVl1leuroJIPimn~Thu~L1ruielEJ~euroJ (UleuroJijn1l1~tha)(bjiin1l1~~ha)

12 O2+ (Sn02_X) -Jgt 0-ad(Sn02_X) (21)

CO+O-ad(Sn02_x)-Jgt CO2+ (Sn02_x)

~~tYUf1I11lJ~1UVl1UtJeuroJl1gtl11Jli11iJ~n1iJ~iiri1Vl~1I ~Imh11~11 fi1f1111lJ~1UVl1U~Ltl~flU1tldil~

LtJUaVlGi1Uf-l n~UnUf1l111lLifll-nU1leuroJIn1l1LtJ1V1lJ1EJ ~IalJn1i

eVe In the presence 0 reducing gas

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 15: 21 .11. 2556 - Ubon Ratchathani University

6

(22)

brleJ Rs A C tm a ~eJ fi1f111lJ~11JVl1U1vh~1lJeJJ~)Il~)~~ fi11ll~ fI11lJbiflJifUlJeJ~ti1l1 UfI~ 1l)1lJiulJeJJn~1~RsIl1lJ~1~~

~1nllrupound1lJ~1iium~Il~)~pound11~LllillJeJJlJn8L~nVl~eJlJm-d ueJmJ1n~Clmj1lJ1Hjum~ 1 U

m)~~n~UVI~eJpound11~~~bVI CJ~1mJfIliImi) tJJClmh1thh~t1nIl1mUeJllpound11V1m~lJmVI1~eJ~1J i U tI

LbVdVIfl1t1 bliU m~1ilJ~lJ1ruUeJfI neJHeJVI~eJn~u1UmVI1~UfI~LIl~eJJ~lJ [14] LiJu~u

23 Gas Chromatography-Mass Spectrometry (GC-MS) [15]

GC-MS LUU16~pound11lJ1~Cl1L1l~1~-HeJ~fitJ~tneJ~1Upound11i~1eJ~1J1iltl mrlfJ miL mfJ~Lli fJJ

Fingerprint lJeJJLfIlJlJ1f1 (Mass number) n~ifeJlJfI~ileJ~1u Library 1ilfJpound11lJ1~Cl1mLIli1~VI1~~~ tUL1lJmlJ1ru (Quantitative analysis) UfI~L1l~flruIl1n (Qualitative analysis) GC-MS

tl~tneJ~~1fJ 2 ~1U ~eJ Gas chromatography Uflt Mass spectrometry

231 Gas Chromatography (GC) Vi1Vnhmum~LLfJneJJfitl~neJ~~1J 11Upound11~~1eJ~1~ ~pound11lJ1imtLVIfJnfl1fJLuuleJ (Volatile organic compounds) 1~LrleJ~nfil11lJ~mJ 1ilfJeJ1~fJ flrupound1lJlNIv1ulJln~1~nuJeJ~eJJfitl~tneJ~lu~)eJ~1Jv1il~eJ Stationary phase Uflt Mobile phase

tl J bull 1iJ~ eJJfil i~neJ~Vl~1filqjJeJJLfil~eJ~ GC pound11lJ1iClU~~t)eJn ~ilL U 3 pound11U ~ilun

1) Injector ~eJ ~1U~pound11~~1eJ~1J~~~n5ilL1~Lfil~eJI UfI~jtLVIfJLUU gas n~eJlJn~~nVi1 1 iJ~ Ii I ~ iJG JI1L ULUeJLilfJ1nuneJIJVltLJ1pound1 column lgtlruVIlllJVlLVIlJ1tpound1lJJeJ~ iniector fil)jL lJlgtlruVIlllJVlpound1~

I ~ I ~ ~ I I

neJ~tvi11ipound11~~1eJ~11pound11lJ1~mtLVIUl~u~~eJ~W~nVirllipound1a1fJ~1 (decompose)

2) Oven ~eJ ~)lJv1~~i column LLfltfil)~fillJeJruVInijlJeJ~ column 1~Ltl~fJIJ1tllJl1lJ J

fI11lJLVIlJ1pound1lJn~pound11iVl~nilil

3) Detector f1eJ~1IJ~ltt -tI~1V1~~Jl~)~1ileJ~fitl~neJ~~ileJ~1Upound11~~1eJ~1J ~IfIJeJ~n~ l1lJillJeJJ detector ~L~eJnlu

232 Mass Spectrometry (MS) LUIJ Detector ll1JilVIj~muJl~1~1~eJJfitl~tneJ~tIJ I 1 1 tJ I 1 pound11~1Jl1eJfJ1J ~fJ lJLfI~fIJeJ~eJ~fil ~tneJ~Vl~nLLfJneJeJnlJ11npound11~1Jl1eJfJ1~ ~fJLfil~eJ~ GC t~n

leJeJeJIJ1IJl11IJpound1n11t1qjqj1mfllLL~)IJl~)1~eJeJnlJ1LUlJLfIJlJ1a (Mass number) LlifJ~n~ tl iJG 1 311IJJeJlJflmlmufltu aNfleJeJnlJ1L IJl1eJJeJleJJfilu~tneJ~IJIJ1

tf ~d tf tl GII m~1Lfil~1tVIeJ~1l u~~neJ~ ~IJpound11~1Jl1eJfJ1~~)fJLfiljeJ~ GC-MS U1JltlJJeJ~VIa1fJ ~tm~ ulJln

iJ~ J I I I 1 0

fJ~L lJLVlfilUfilVllJ~1fil1LLn~UfltIl11oll1fJ lJmj~1j~~n~mll~eJ~pound11 I

etcl241 flLtlULtl

~Li1IJLeJ (DNA Deoxyribonucleic acid) LUIJpound11j~lJtmj~lJJeJ~~Jil51Jl pound11lJ1~Cll1aeJI

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 16: 21 .11. 2556 - Ubon Ratchathani University

7

~1LEjL~ ~LBULEViTVIj1~LihJ~~i1~ElJ~lJEjELUJLL~~ri111V1EflHU~n ~LBULmh~nEtJ~111V1Lbl1 J I

VEI1V1iELtJi1 4 i1iJfl L~LLri A (adenine) T (thymine) C (cytosine) LL~~ G (guanine) LiI1j~EnU

L~U i11111111 iVl~VlnU1Vll1ijLLnUL~UJ1(l1~~EEnih1tJi1 (deoxyribose) LL~vlEi1Lvl(l

(phosphate) i1111fJ11dLil1n11i11IJibflffiELV1fl (nucleotide) ~LBULEL~Ui11mj1flffiELV1fl 2 i1111

~tJnUL~ULn~m~lflULtJi1~1jl m~~tJ nUlJEjLtJi11U~~tJnULLtJtJ L1ll11~L~1~j1VlI1~Uli~Lflij LtJi1

A ~~~tJfintJLtJi1 T LL~~LtJi1 C ~~~tJfintJLtJi1 G yh1~~LBULEijjtl~1jfl~1I1UULflL111U (spiral) mj ~ ~ ~

L~E1j~1lJEjLtJi1 A T C LL~~ G ~tlj~nEtJL~Ui111J~L)ULEijf111lJff1rtylJ1n Ll1j1~1U~~fl~~L~U~1 t11V1Uflmj LLtl~fl11lJVllJ11JEEnlJ1LUjtllJEj1tlj~U~L~Umfitli~ nEtJlJEj~jij1Jl~1jl ~L)ULE

~

lJEj~i11Um~~1I1EEllVVI~1ULLVlcijJl111LULflj~t1 L~LLri LUiJ1Lfl~m1 (nucleus) bJ1JlflEULfl~1J ~

(Mitochondria) LL~fl~Ehl1~1i1~ (chloropLast) 1fllJ~1LtJ1Umi~flVllJ1VlVl~ (classification)

LL~~Vl1fl11lJ~lJ~U5V11j~UqmjlJ (phylogeny) lJEj~i1i1iJfl~1j 1 iJEllJLobfiLBULElufl~Ehl1~1i11Ji (chloroplast DNA)

~ If Q ~4-

243 LWlftLIftn1U11~a191UW~LEULi)

L~lJtAU~1E1n1n~Ui11aV1gt3~Uil1lL~U-ni1i1flVlia-nl1LL~jmvi 51L~u-nl1i1flfl1iLilanlUEiEU fl1iL~U LLl1ntAU uElnuVlcigt3tl~n~1E151 L~ULtJl~ mii1i1~~1E1fjlJ~~L8ULai11lJ1ilLLv~aam~ULLtJtJ~1gt3l ltIV fl~U

1) Polymerase chain reaction (PCR) - based methods

L~LI1~~ijmilobtlijffiEl1~n1jl1E~LlJELi1 (polymerase chain reaction) tUiUJlEulfl

iUJlaUVlii-3IJEgt3mna1E1fjmmBULahlJ~1E1 VInmiIJagt3 PCR fia n1~gt3Lfli1~VIm~~L8ULa i11111Vl1imlJ1ru~-311UtUVIaaflVIfl~a~ 1flEl~~tAU~1n~LBULatAuutJU (template DNA) t UmlJ1ru

L~njaEl ~gt3ija1UtIimEU1rGt 3 a1U L~uri a) Ll1iwai (primers) ~~L~UiJ1f1maLVIiii11E1~U b) LauLl1 DNA polymerase L~ULEULfljl1~li1E1tUmit1LUi1~1~ 1 lJ1~anUVi11~i11E1fiLBULa m1~ULLa~ c) iJ1flmELVIii~-3 4 l1iJfl fiE dATP dCTP dGTP U~~ dTTP t~lobi1i1jfiLBULEi11E1 t VI1i mi1i1~~1I1fjlJ~~LBULa1flI11~~ijmi1obtlijffim~n1lil1E~LlJE Lii1~1lJ~1E1di11lJ1iflUV-3EEn

L~~m~uVI~1E1UUUL~LLri RAPD (Rapid Amplified Polymorphic DNA) PCR-RFLP (PCR shy

Restriction Fragment Length Polymorphism) AFLP (Amplified Fragment Length

Polymorphism) SSR (Simple Sequence Repeat Minisatellites Microsatellites) L~UIiU

2) Hybridization - based methods

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 17: 21 .11. 2556 - Ubon Ratchathani University

8

VIa~~1nll~~hBULa~bEJ LeJUli1~Il~ij1Lvnt LL~tLLEJniU~hBULa~1EJ~L~n1~1 -naLLa1 ta~ ti1EJeuroU~LBULmVI1rU1tJl1UULL~UYJm~ai (filter membrane) LLa11hlJ1~U (hybridize) tlU1l1

~1~~U (probe) vl~~illn1fl~1EJtlllirU~-rlftVI~aa1Lflii uja1-dLaUli1l11lij~mj~Il~~LBULaijalV41l Ilij~VI~~ ~t1~euroU~LBU LailiiijU1~Lmt ij1U1UfI~ilLalla ti1~LBULa ijalV41l1i1~tlULb~tii1~UL Uail

Ltl~EJUbLtl~~1tl Lrimj11111l~~1maUli1l1Il~-51LW1tllij~L~EJ1nU ~t1~1lU1~bL~t-51U1UeuroU~LBULavi LL~nIi1~~1m~1I L~EJn11Ln~Wa~lIaiYJ1I (polymorphism) VI~a RFLP U

3) Sequencing - based methods

nTlvi1~1EJV1I~~bBUba1~EJnTm1h~ULUaLiJU15via1111fI~1~aaUfl1111LL~n 1i1~ija ~ 1middot 11f~ fQf ~ ~ ~I ~ WIl ~amllntbaEJ~ a1lJ1flVI1U ~fI~fl11lJLL~n~111la~~LeJULaVla1~LuULLUU mLLVIUVlnUtIa~

LUi (substitution) mL~lJtIalLUa (insertion) VI~amtI1~VI1EJ1tl1la~LUa (deLetion) a~lnm~ irnj1lJ1~~vi1LiJUi1EJfl1111~1I~U~V111~U~mlJijal~~iim~ (phylogenetic tree) ~IEl1~~~1~ 11LtJUln1EJV1J~Iij~VI~I ma~1~~1EJVlJ~~L~ULa1VCJmVI1a1~ULUamuYru~~ L~a~~1ntaltu

cO a1Lfl1JLL~~LfIallJaillll1f1r ILLW~

ln1EJV1J~LBULavlflna~11~UrUm~VIln1CJtlLLUU~UnUil~~EJVI~1EJifij~ j111~ILfI~f)Iiia~ ~

LL~~LV1f1Uflvtlu~LtJUU~~EJ~It Umjn1V1U~jtlLLLLUUtIf)~~1EJV1J~~L~ULam~ mjj1ln1EJ~1J1tl

t It 1 1 I

~

iJ bull ~ifflLIJUa nu~~EJVIU~11~~ ifLnam~j1~aaUVISmaLnUL Uija~~LV11UU

2SLlIaUfla1J LabVIEW

1tlLLm1J LabVIEW (Laboratory VirtuaL Instrument Engineering Workbench) LtJU

1tljLLm1Jvtl-ntlU1J1ntUVl11mS1~LL~~~UUfl1UfI~I1tljLLm1Jvl~WJU11~EJ1i1tljLLm1J LabVIEWbull ~~L~EJn11 Virtual Instrument (VI) VI1J1EJalLfI~aliia1~LaiiaU ~I1mUm~~L~Ua1Jln

~

tl~1J1~~ln LLln~LLi~~a1Jlnvlij1LiJULia91~liatlUmu 1~EJ1 tljLLm1J LabVIEW ~~vi111ULLUU ~ ~

Dataflow ~IWL VIiiaUtlU 1 tljLLm1Jtl~EJn~~Uvlvi1~1U~1flUUln~~11 b-tlU Pascal C LtJU~U 1flEJbull LabVIEW UEJ1J1m Umj91~liatlULfI~a~iiaVI~aVlS1Ua~1bilaiLY1a1u1~af1Jqf1ruVl11mEJfl1n1i11 1

26 Principal Component Analysis (PCA)

LVlflUfI PCA LtJUmjLLtl~~Lj~rlil1n (Orthogonal transformation) LVlflUfIVIjl~I~~vi1mS LLtl~I~1LLtlj~1nmj~~Ln~VI~amj1~1tl f11vn~1mJVI~aalr1tlStnaU~~1flf1J (Principa L

component ) v1LiJUilaS~~anU 1~EJvia~r1tlS~nf)U~1l~1flf1JVIanIl1LLjn (First principal

component PCl ) ~~iif11f1111JLLtlStlS1U (Variance) a~via~LL~~~1LLtljVIan~1tr~1tl~~iif11~

fl111JLLtljtlS1U~tlVI~UtlU~~1tl LVlflUfI PCA nUEJ1J1mUmSij1LLU na~~LLUU Unsupervised

bL~~1 m Umj~~U1~alall~naUh1tltlj~lJ1~~ln~atliJn~1EJ all~vij1tl1LfIj1~~~1 EJ ~ ~

bV1f1UfI PCA dbJUf11LLSIviU~1m~~j1~alboftUbIai~~~1~1m~~1nmS1~n~Ua~nS~LViull

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 18: 21 .11. 2556 - Ubon Ratchathani University

bull bull

UVIVI 3

rieltl~~~mh1t1mljltil n~tllm~mbYltJ~ 1i11tJ~~nBb~ nVl~eliJ fl~rtl 1tluVld~~na11t1J fl1~ IJ

OJ cv ( cv q laquoV d I OJt CV J 6

91 ~1 ~Vl1l11~61~VitlDVl1 ~VitlDm~~lJ elJ m ~bVl tI~911 euroJCn ~bVi el tltl tJUrn1~ bb91 n m ~ ~~Vl11JVitlD

m~bYltJ~~1t1 fl1~ I~1~hJ1fl~ 6tlamp1 nVl~bbaVl~61elU61~~amp1~1tlhm6YltJ ~t911 ~~U5nU~ij

elJyJtJ~n elUVl1J blijVl~ eln~U~bb91Ilt911JnU 1~tJV11fl1~Vl~G1 el~Vl1el ~yJtJ~1l eJUVl1J blmtll1~~ bVltJ

lJelJmbYltJ~~tl~t911~ 1 ~1tJfl1~1bl~1~ GC-MS 911~~1~U

31 nTHihJ~Jenh~mbVimJ

lUfftlfl1~VltilaeJ~lvit1~1eJ~1~mLYltJ~1UtJ~~LVl1l1VltJ~1n 3 LLVla~ l~bLri eJJJ1~lfw~mJ ~ 1I~61LIlli eJJ1tJ1~ ~eJ9l~~9ltl Lba eJthtl1ri~ ~~1ViU ~nUtlbbVla~tJG1llmLYltJ~~ij~m~m

IJ IJ

~ILLlIill~1eJlhlmLVitJw1U~tJ~ 31 b~eJl11~b~mtUh~l~m~LYltJ~~U5~tJG11l1tlVfeJI~tlatl~~JIJ IJ

fl1~]IilVl1mbYltJ~ bVla1d~~eJ1IiltJ9l~I~11lb1lli91~mvi~]n1tl~tlviatl 1 mV111l1~bnuieJ~G1~1tJ15 t911J 1

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 19: 21 .11. 2556 - Ubon Ratchathani University

10

II C~ d CIt

32 nTUI111a1flVUJWflLtlULtltltlJmLVlfl1l

LitlVlS1il~tltJfl1111~1I~U~Vl1J~U~mS1I (PhyLogeny) SIl1JmLYitJ1Il1tl~1J~1I1il1n LLVliiiJJan~1J tiU ~JUl1mhJnSLYitJ1I~JVl1Ifl~Jflmj11JVl1mtJYl1l-W~lLBULtl ru fl1fl1l11~Ut)

U I U

f111~fli flru1VltJ1f111~V1i 1IVl1iVltJ1tltJLm~mfll1~fli ~1tJ1fi AFLP (Amplified Fragment

Length Polymorphism)

ifi AFLP QLtJUmS1-uLVlflUfl RFLP LLG PCR L-rr1~1tJtiU Lflv1-ULtlU1~21lfl~1LVnlfl~iLaU LtlritlULLaL~tl1l~tl~LaULtl~1tJ adapter ~LtJUU1flruB1Vl~~1mtUbJLnU 20 LtJ~ LLa1~JL~1I m1l1ru~1poundJ PCR LflpoundJ1ViiL1IBiil~tJL1lVi1Lil1ilJtitJ adapter U1 il1mjU~JLLtJn~UbUtlBJ~ LBULBlflpoundJl-uViB~BfiG111l~LJG LLaJtJS1nl~Uri1UIIm~LaU LelLtJULLIltJ

3211 n11flMlflLtlULtl

1 hmLYiEl1ltJfltULn~J~LVU lfltJL~1IlU1V1SLilULVlG1LLa1tJfl1~aLEElflJULtJUtH 2 U1~1B~1j~tJflLLa11~1UVlGBflllU1fl 15 ijG~~flS L~1ItJtJLVlBim1l1V1S 600 11l1fl1~V1S

(CTAB 2 NaCl14 mM PVP 2 EDTA pH BO 20 mM Tris HCl pH BO 100 mM

LLG 2-mercaptoethanol 2) ~J~Uv1~WVIfJij 65 BJfII1L~aL~El~ Lh1Jti1lv1~wVIfJii 65

tljPl1LlIaL~m LtJUL1G1 60 U1Yi LflpoundJmrtJVlGBfl1J1I1 2-3 flfJVln 15 U1~bull 3 L~1Itl11aG1EllltLLVl~L~poundJ1Itl~LVIVl m1l1V1S 300 11l1flSfiVlS tI~1It~Lli1tiUlflElLtlEh LL1J 1

LLa1LL-r11UJ1LL~JLtlUnG1 30 U1Yi LUffUmJ~ULLalVi~LL1Iflfl1l~~ilV1nVl~ntlU~11JtitJ lltLLVl~L~El1JlflLfl~aaGLVlVl LVI~BmflU1fl~Hintl~IU~1SaG1poundJ u11J~ULVI~poundJjv1fl1111L11

13000 atJ~BU1Yi LtJUnm 30 U1Vi

4 ~fl~11f1Zfl1ElU1ri1UtJUm1l1VlS 600 11l1fl1~meJ1EllriViflaflLVIli L~1Iflaal1tiBi1J 1al11

Laiia (chloroformisoamyl 241) 111111911 600 11l1fl1~VlS tI~1IL~LtlULltlL~El1tiULflEl n~JVlaBfllJll1J1t1l1ru 20 U1Yi u11J~ULVI~Eljv1fl111JL~1 13000 StltJ~BU1Yi LtlUL1f11

10 U1Vi

5 ~fltl11f1zG1Ell~ri1UtJUm1l1Vl1 600 11lLfl1~V1SeJ1tJlriVlfltlflL Vlli L~1Itl11aZa1El

ll1LfiEl1JaZ~LVlVl pH 52 m1l1Vl1 50 11lLfl1fi911LlJEhLtJ1 1 LLa1L~1JLaVl1UBa 95 ~LVU ~flm1J1911 2 Lvi1lJtlj~11f1fl1ElLUVlGBfl U1lJti1J~aruViflii -20 tlJPl1L1IGL~Eltl LtJUL1a1 15 U1Yi L~aVlnVlznaUfiLBULa u11J~ULVI~poundJJv1fl111JL~1 13000 1atJ~aU1Yi LtJUL1a1

10 U1Yi I ~ 1111 _co( ~ ~4

6 LYI~11f1ta1V~1UtJUVlj LLfl1(t1JVlznaUflLaULB()1VLBVl1UBG 70 2 fl1J 1iI1nUUtIJ J 1 II ad

V1ZnaUflLaULaVlaWVlfl1l 37 tlJfII1L1IaL1IEl~ LuUnm 60 U1Vl LL(t1at(t1ElVltnBUflLtlULa 1EltJtJLtiai TE (10 mM Tris-HCl 1 mM EDTA pH BO) 111111911 20-50 11l1fl1~Vl1

ltII

LViaaZa1poundJVltnBlJ()LBULeJ

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 20: 21 .11. 2556 - Ubon Ratchathani University

11

3212 fl1~1Lfl~1~-MLilLilWuilai1 (AFLP)

t11~LeLtLtl~~mfl1~~l1~atluvhtJllmtn~1lJ~U~mJ~ji1 1 ml~~V)1ULtlUl1l1~~1ii1LVl1~LLa~litl adapter 1-if~LeULtl~ULLuU~~tljm~~~1~atlu9i~

v)1milUl1l1~~r1LVi1 2 lIij~Vl~il1JnU fltl fcoRI ~jLtJULtlUl1l19i~r1LVl1~ijfl111JmU ml~~~1 ijvi1LLVlUj~~r1 6 Lua LLa

I LtlUl1l1 Msel ~ijfl11lJmUml~~iI-1 ijvi1LLVlUj

~~1ii1 4 Lua tJa~1nml9i~Vl1mtlUl1l1-vYj 2 lIij~ ~1V)lIU1~~U~LeULil~LLVlnIi1jnU 3

lIij~ flil (1) ~U~ijtJa1t1-vYjililjVl1UQn~Vlv)1milUl1l1 fcoRI (2) ~U~iitJa1t1-vYjililjVl1U Cln~VlVl1ULtlUl1l1 MselLLa~ (3) ~U~ijtJa1t1~1UVIjjCln9i~Vl1mtlUl11i fcoRI LLaiinVl1U I I

VIjjjn9iVlVl1milUl1l1 Msel ~1mrUt11 fcoRI adapter LLa Msel adapter ~-1LtJU~U~ LeULtlil1poundJ~GfU 1 lJ1IiilLltU1nutJa1poundJ-vYj 2 Vl1UlItlj~U~LeULilmv)~1nml9i~Vl1ULtlU11l1

~~-j1LVl1 L~tllgLtJUvi1LLVluj~utJil-31~Wil1iUtJllmpoundJ1ml~-3Lfll111~LeULtl lVltlml

j1~L~ULtl~ULLUU 100-150 U11UnflJ )J19i~~bUtJa1poundJV)1milUl1l1 fcoRI LLa Msel

tfij~a 5 ~ijVl ~tllJiiUI0x VULYlili A 50 bJlfll~fll ~illJ-vY-3L9ilJLilUli1l1 T4 DNA

ligase 1 ~ijfl 10 mM ATP 15 bJlfllafll ~1lJiiU fcoRI adapter LLa Msel adapter

mlJ1W 5 LLa~ 50 ~lflllJafl1lJ~1viUL~ilLtJUmllJiilLtllJ adapter LltU1nUtJa1poundJ~LeULil ~ULLUU tJiUmlJ1fllilVI5LtJU 30 bJlfllafllfl1mh deionized t111tJjlJ~tlWVlflii 37

euroNIfl1LtUaLilViI U1UtJ1lJflU

2 nT~vh-n~il1i I VI~El preselective amplification L~ElL~lJmlJ1WflL~ULElLtl1~bU~ ~El-3ml vhIf1vt11flLeULEl~~flVl1ULtlUli1l1~fI-j1LW1~ LLa~IJiElnU adapter L~poundJU~ElVLL~1lJ1 L~El~1-3a-3 10 LvhVlmJ1 deionize luflLeULEl~L~El~1-3LL~1mlJ1Vll 10 bJlfllafll Li)U

~ULLUU i1tJfiffitn-n~tl1inU fcoRI llwEli LLa Msel lWlLlJEli~L~lJLUiI~VlLaEln~ tJa1v 3 -jlU1U 1 LUiI L~ElL~lJfl11lJr1L1L~1~-31Uml~VlLaEln~UflL~ULtl tfijVla~ 05 bJlfllafll (5 bJlfllllJaIJiEl28bJlfllafll) ~EllJiiU lOx VULYlai(10 mM Tris HCl

pH 80 50 mM KCl) 10 bJlfl~afll 15 mM MgCl2 06 bJlf1~afll dNTP 20

bJlf1lal (1 bJlfllllJaIJiElllJlf1laVll) uaLElUli1l1 Taq polymerase 02 bJlf1lafll (5

~ijIJiElwlf1lafl) tJiumlJ1flllVI5LijU 10 bJlf1la~~Vl1poundJJ1deionjze LL~1~-31111tJlh tJfiffitn1U LfI~El-3L~lJm)J1WillU-wU~nl~lJtfij~fl1UllJ~wVlJJiiafllUml l~v1mtJiLLnllJ

lUfl1j1tJiffim-n~El1i I vi-31 28 iElU denature ~~wVlJJii 94 El-3f11LilaLiBviI 15 q ell ~ __

1UlV1 annealing VI~WVlJJlJ 60 El-3f11LtUaLilViI 30 1UlV1 extension VI~WVlJJlJ 72 El-1f11 4J _ _ltAI Ad

LilaLtUViI 60 1U1V1 1 iElU extension VIElWVlfllJ 72 El-1fo11LtUaLtUViI 120 1U1V1 tJatJa~V1 bull I

1~~1nmii1 preselective amplification fiEl miL~lJ-jlU1UtJEl-35UflLeULElLtlVi1~~ii tJa1VVl1UVIj-3(ln~~V)1t1 fcoRI LLa tJa1viinV)1UVIj-3Cln~~~1poundJ MsellUffUflilUi1~lbtl

1 I

a~-j1U1U11JflLeULEl~~El-3mi~~LaElna-3 5U~L~ULElmVliUmiL~lJmlJlwlUrrUVlElUi1~ HjLjUflLe1JLEl~1JLLutllUmivi1 selective amplification IiElltJ

3 mivi1Yf~il1i II VI~tl selective amplification li~LBULEl~1nYfiBtl1i I ~L~El~I-3a-3 10

LIh~1VJl deionized LtJU~ULLtIULUmivi1tJllffitn 1~tl1-nflLeULEl~L~El~1-3LL~1tJ~lJ1~i

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 21: 21 .11. 2556 - Ubon Ratchathani University

12

10 wlf1~iJl~ LtJlJ~ULUU vi1IJ~n~fJ1nU EcoR11Vi~LlJ(li LLCl~ MseI1Vi~LlJ(li~L~lJLU ~V1L~(ln~ lJCl1a 3 ~1lJ1lJ 3 LUa L~(lL~lJfl11lJ~1LVi1L1il11iljlum~FiV1L~(ln~lJ~L~lJL(l lIij~Cl~ 05 1lJlf1~im (5 1lJlf1~llJCl~(l1lJlf1~iJl~) Vi~(llJnU lOx liULYl(li(1o mM Tris

HCl pH 80 50 mM KCl) 10 bJlf1~im 15 mM MgCl2 06 1lJlf1~iJl~ dNTP 20

1lJlf1~iJl~ (1 wlf1~1lJClti(l1lJlf1~iJl~) LLClL(llJ1~11 Toq polymerase 02 1lJlf1~~Jl~ (5

fJijJl~(l1lJIf1~iJl~) IJ~UIJ~lJ1Jl~aYl5LijlJ 1 0 bJlf1~~Jl~~1fJh deionize LLth~jl11IJV11 ~ lJnffifJ11lJLfI~eNL~lJmlJ1rua1~~lJfm~~lJlIijVlmUfllJ(l ruVIllije)JlllJiWi lV1fJ1mIJ~LLmlJoJ I

llJm~vi1IJnmmVi(l1i II t1j113 ~(lU denature ~(lruVIllij 94 (ljf1LlIClLfJa 10 ~ ~

1lJTVi annealino ~(lruVIllij 65 (ljf1LlIClLim1 30 l1nVi (ClVl(lruVIllijClj 07 (ljf1

I ~ bull cJ ~ QI QQ

L~ClLlIaaJlm(lUVIClj~1ma~~l(lULL~n) extension VI(lruVIlllJ 72 (ljf1LlIClLlIfJa 60 1lJ1V1 ~

J _ aQcIi ~ ltQ

25 l(lU denature VIeuroruVIJJlJ 94 (llf1LlIClL~fJa 10 1lJ1V1 annealing VIeuroruVIJJlJ 56

(llPl1LlIClLiEJa 30 llJ111 extension ~(lruVIllij 72 (ljf1LlImifJa 60 llJ111 (L~lJL1Cl1~(lU ~

Cl~ 1 llJ1Vi)

4 m~LLfJn~L~lJL(llV1El denaturing polyacrylamide gel

mn~ElllmmhM~uLnLft 1) j1LLNlJm~~n1V1~ULnL~CllJ1a111~az(l1~ LLa1Lj~~1m(lfi1lJ(lCl 95 t~azm~

jl 2 LLNlJ

2) L~~mZ~nLLNlJVII~1fJ bind silane (bind silane 1 1lJlf1~iJl~ glacial acetic

acid 25 Wlfl~ii LLClZ L(lfi1lJ(lCl 95 500 1lJlf1li~) L+1(lt~L~ClLmz~V1nU mZJn

t II cia tt bull ~ II ~ II 11 3) nizJnwllJVIlJ1V1lJCln1tfUZLUlJVImZJl1a LlIVI ~VIVI1V11fJ clear view LVi(lu(ljn1J

Wt~LJCl Lm~~~nUmz~n lJa(lat~LL~jt1iZlJ1ru 5-10 lJ1Vi

4) j1niZ~n~I 2 LLNlJlJ1IJiZn(lULu1-q~nlJ 1~m11 spacer H~la(llu11L+1(lt~Ln~ j(lli11izwhlnlz~n~la(lj 1~fJilJ~1lJ~VI1 bind silane LLClZ clear view Lu1V11

nlJ tufliJVIjUfjill~(l~fII~ 5) L~alJlil(lzfI~Cl1bJ~L~ClLlilJulJ 45 LJ()iL-lIlJlJ 1~aj1 45 acrylamide gel

40 Wlf1iiJli ~alJnUa1lClZCl1aLL(llJUJLjfJlJLJ(li~ClLYlJlLlilJulJ 10 250

Wlf1ii(JI~ LLClZ TEMED (NNNN-tetramethylethyLenediamide) 20

Wlf1ii(JIi ~alJtlJlJ1i11V1~ULVIL~Clt~Lu1nlJ iZ11(l~11t~Ln~(llmmPl

6) LnL~CltaCljt lJj(lliwhjm~n~lJL~lJ iZ11(l~1t ~Lnil(llmn1P1 LLa11aVI1 Lu

J1lJUlJ Ja(lElt~L~ClLL~I~1thlJ1ru 2 ff1WI

3213 mithaLflfllV1llra

1) Li1(lLJClLL~I~1~LLa11-nJa1I1l~z~nIl1lJlJ(ln1~a~mVl ~jVI1(l(ln LLClzJin(lUm~mli1

nULfI~(llaLClfllV1~lmia LLClL~lJUULYl(li lx TBE (100 mM Tris HCl pH 80 10mM

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 22: 21 .11. 2556 - Ubon Ratchathani University

13

m~n

2) ~fl~1tJlvkti1nuLfl~rN~LafllV1Jlm~~ LtJVlmm~1rlvhvT1 pre-run ~fi1~lrlvh 60 1t11vl

LhJL1~1 20-30 l1V1 ~lJijflruVIniitlJ1J1ru 45 fl~fI11Lil~L~lI~ bull I

3) tJVlLfl~fl~ HfLa1JaVlll1VlVlUrlLrlfli1l11~~1V1j1llfl~L~~ lciVI~hLUU~lJil~1111VltJ1~VI1~1lJ~ I bull

LtJlJ~lJ~~1tlaller~nU~1V1j1L~~L~tJ~Lanjflll LL~1tlL1i1J5VltJ1~VlUrlLrlfli1~~L~jVl-dfl~

VI1LL~~iifl~flfln1~VIlIVl

4) VIlIflVl~1fl~1~~M)lJLfl~1nn1Jvi1 peR 1 ~L~lI sequencing dye (98 formamide 10

mM EDTA 001 (wv) bromophenol blue 001 (wv) xylene cyanal) 5

bJlflJ~IJIJ tR1I11J1J 4 bJlflJalJlJ ~-31lJLL~~VI1l1L~~ ~1nrlJLtJVlLfl~fl~iiL~fllV1Jlrl~~~ lVlllltlfh-31rlfl-3~ 60 11J1vl LfJlJL1~1 80 lJ1V1 ~lJn11~ xylene cyanol (~~fl~~1lJUlJ) Lfl~fllJ~~~1I1tlJ1I1ru 21lJ 3 ci1lJIIfl~L~~ 1 aIIIIJ f II 0 aI t f

5) uVlLflJfl-3 LVIU n LllflJ~1nlffl-3Vl1lJUlJflfln lJ1m~nflfln~1mflJfl-3 LLlInnJ~ nVl-3~fl~LLjJlJ IJ I 0 1tlll II

flfln~1nnlJ L~~~IJIVlnum~nLLjJlJVI~-3V1L1JlJ~LVI~lIlIlJIJ~ lJ1L~~ L EJflll~mV~1J~~1j

~~L1flfllJLIJIJVI

3214 n1fIJ1)flaJuaJfiL~uLaA1fJfl1Ja=afJ~aL1ai1uLfIJVI 1) hmtl~m~~1I1LII~htlJ~1J~~1EJmVleJ~jmu1lulJ 10 LiJlJL1~1 30 lJ1V1 LL~ 1~

tI ~ t ~ mEJlJ1n~lJ 2 flJ~ flJ~~ 3 lJ1V1 UlJLflJeJ~LIIEJ1

2) j1LL~lJm~m~~VeJlI~1EJ~1J~~1EJ~~L1OfluLI1IJVI (1 silver strain 056

formaldehyde) LJOVeJlIL~~LfJuL1a1 30 U1V1 mnjVtLtl~~L1flfllJLI1IJVI~1eJ1~L~1JL1ml~ ~lJil~ 1 i111J~ LU~1eJ~1~~~1L~1IOfI~eJVlL1~1 C11VlvtitlVeJ1I1~L1eJfujfl1J1tlC11VlL~lI1nu fixation LYtJ1eJ1~ijn1JtllJL~eJUUeJ~nJVleJ~jn ~1~VeJlIl~~

3) hLL~lJm~m~~fleJn1~fl1mi1 deionized fl~1-3J1VlL~1LYleJ1~~~L1flfllJLIJIJVlci1lJLnlJ flilfi

4) hu~um~m~~t~~~tlJ~1s~~1EJ develop (30 sodium carbonate 01 sodium

thiosulfate 056 formaldehyde) ~LIJI~lIlIlV11iLL~LL-dL~lJ~~ruVIilij -20 fl~m Llf~LEJ~LII~1O~1-3~~1L~lIeJtls1I1ru 5-10 lJ1V1 ijJ~Jfl-3L~uLmU~L~lJLeJ-KVlLijlJ

5) VI~VltlnmEJ11VlEJj1LL~lJmijm~~t~ilJ~1~~~1EJmVlfl~iin 10 LfJlJL1~1 3- 5 lJ1V1

~1tfl1V1i1 deionized 10 lJ1Vi ~tll1LLl1-3tlJfl1n1f11 6) hLLlJum~n1l11Lfl1~jJ~

322 Uiln1SVIVlilOt

~1fl~1tmLV1VlImmUn1~VlVl~fl~jij~~VIlIVl 6 ~1mht l~LLri mLV1EJ1JfI1~~LnM m~ ~1nLLVIei~tl~n~ 1 (SKi) LL~ LLVIei~tlin~ 2 (SK2) mLVillllLiv~1mi (eM) mLVivlI(hrllJ (LP)

I I I

mLViEJlI~I1IJ~lvl (UT) LL~mLViEJlI~U~VI1~fl~1nVffltI1l~1Vl (eN) lVlEJvi1n1VlVlaeJt~1~1flci1~

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 23: 21 .11. 2556 - Ubon Ratchathani University

14

~ 3 flfj ~jlu1~jL~tJti1ntl~1~tlflfjtJtI-lLL~~~1t1~1jmLVimJ poundJn~1t1~1jL-dU SKl 2

V1lJ1poundJ(i-l ~1t1~1-3mLVipoundJlJfI~1Ln~l1mLV1Gl-3t1~n~ 1 ~1~tl~ 2 rJ~miVI~~tI-3mJl11lJ1middotmU1lJ1

G1-1-31yf[~~LU~nVI~LLtltlih1nl~poundJ1~ UPGMA (Unwei~hted pair-group method with

arithmetic mean) lJ1~j~tI~ 32

CN_2 UT_3 LP _2 UT_2 CN_3 UT_1 LP_1 LP_3 CM_3 CM_1 CM_2

100

SK1_3 SK2_3 SK2_1

SK2_2

2S

Zl

31

CN_1 SK1_1 SK1_2

91

323 1~1middotnl~iln1VIf1iltl~

LW11nLiiiEJlJ~1t1~1-3~ih~jV1lJfl1UmVI~~tI-3~lij 6 ~1t1~1-3J11EJnU LLIJItU~~ltJtI T4l1nn~~miVI~~tI-3LQrntJtI-3nLl1poundJlJf1~aLn~ ~1~U uiltllJIi~~ciLVhrU Li1t1T4l1i(1J11y((il~ LU~nVI utltlih1n1Uitl~ 32 ~L~U11M-3n1UhUGl1-3rUijLL~n~tIU (taxon) LtJUmLVi poundJlJfI~1 shy

Ln~~-3V1l1f1 1~poundJ~1t1~1-3mLl1allf1aLn~~1nLL~~LLVlGl-3t1 ~nllfl11lJ 1mJflnU u~l1n1y((~~

LU~na1lJ1i(lnGl111f111mLl1poundJlI~Utif1~1 Ln~ijfl11111n~J~ntlniLl1poundJlI~Uti~U 1 1U~tl~1 1 middot 3 J shyaI

VIitln~11 ~11mLVIJ2Jf1iLn~2J~U~m2J1-3~1nmLVIpoundJ2J1n~UVIn1flLVl1JtI Wtl~1iru1

mLl1poundJ2J11Jn~1ln1flLVliltl l~tl11a12J1(lLLpoundJnmLl1Elll 11mja-31VI~euroltInl1nm L l1poundJlll1n~1~U

LL~tI~lJImJltl~1-3-U~LlU 1UtJruvlnLl1a2J~1Yt1Ju~tlfI~~cijjfl112J1n~a~nU1Ui~tlvla-3Lnfl 111 u~1~112J1(l11t11J111LjU~UtiL~a1nU

33 n11~fI1ltMtl4fthmtltlt1Ja1LMti II bull ltf ltttl LYt8LU1Jn11V1~1tJtl2J2J~11JVI11nLVIfJll u~~~U~2J tI-3fl in8tl LUa1iiLVlEl

LLIJI n~1-3nU~-3hmiIJli111t1tl tI-3fltlintltllU11llLVI fJtJ 8-3 u~~~1 tI~1-3 J11fJ Lfl~tIj GC-MS ~ -HtI-3t1~mn1lU8jn1fl1fn1f11ml2JLflllLL~~jLL1~~tlll flru1f11ml2Jfl11~ lJVl11V1poundJ1~El ~tl~l1ffl)1il 1UmiL~~fJlJ~1t1~1jVliul~J11 mfl~tIj GC-MS rU~vhmlan~LeiUl~Ell-n lUl~nlULVI~1 1~fJlULQtIj~UlmnfJtlLl1poundJtl1-HL~1Jfl1111ufln~1JtJtIJmlJ1wtJ8j8Jfitllntltl11J

bull qI ~ II ~ ctI _ a n~U1I8Jm L VIpoundJll UUU12J~VIijl1nml1~~1fJ GC-MS 11nUUmiVI~a8tlIij1L~tlVl11Lfll1VlLffj

m1l1rull11L~lIL~lJ~ tiltI

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 24: 21 .11. 2556 - Ubon Ratchathani University

15

nTH(1 itllJlJl1 tJtnl

hmnViCJlJ~~~atJmtlatJntJtJnua~tJ~~lfJ-UtJlJ~Ua~Li)t1~mlJlru 1 g tJll~allUll1~ ~lmjUL~lJ Oichloromethane 50 mL bjtJ~n~tJIfltll~ntJtJVll1LflljllmmbVitllJ bLa111n1l

mtJI~1t11UatJUViaL(1tJillU1~ 02 um U1~1lmaltJ~mtJIU~1tlllJlru 2 uL 5~dh~Lfl~tJI GC

- MS 1~aL(1~CJlJ~1tJcJ11~1laa1tJlltJImLVitJlJLL~a~U~ 1 a 3 ~1tJcJ11

GC- MS

Lfl~tJI GC - MS m-ue-Ja(11~tJtJ~~Vl Agilent ~ItllntJtJ~1tJ Network GC system iU

6890N ua Mass selective detector iU 59731~t11-ufltJ~lJJ 08-5 ual-u He LtJUhfllW1~1t1bull fl~j1n1j1V1a 51 cmsec uatJruVlnijllmL~1tJtJ~L~lJ~1n 50degC ilUll 150 degc ~1tJfl(1j1 5degCmin uafllfhH~ 150degC LtJUb1a1 3 U11 il1ntutJruVlnijilL~lJltlLiju 250 degc ~1tJfl(1j1 30degCmin LLafllfhHvI 250degC LJUL1a1 1 U1Vi lV1lJ~ electron ionization (EO ~IfhH~ 70

eV

_tot JCIII

~1nn1~VlatJIQ~1a a1VVl~nflil1nmLVlVlJ LtI1LflleN GC-MS Vl1lJllU~tIUVlna11lJ1

ltU11iU ~~elamjVlflatllmfiilLL~~1v1tJtltIUlf1lJ11~LLn~lJ (Ion chromatogram) lvunU

UtJuLL~~d1JtJb1a1uaLLnUi~LL~ILtltliL~U Abundance tItJItI~f1tlniltJlU~1~am1tJ l~tJ I 0 ( j J lot v bullbull1

LLtJlatI~fl uniltJ ~U~1aa1U~lJ911LLVIU~ltfl Peak) YlLL~n~11nU LLaYfUVl L~m1 lltJ~Yfflil

LL~VIIl~mlJ1iulliNillfltlntltJrU ltlil 33 - 35 LL~VII1tJtJtJUlfllJ11~umlJtItH~1tJcJ1~mLVi~lJl1nfll~~Hnl flflvl 1 2

ua 3 tJl1lJd1JtJ

Administrator
Oval
Administrator
Rectangle

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 25: 21 .11. 2556 - Ubon Ratchathani University

16

itJYi 36 - 38 LIZ1f1~1~~~1I1Ri1J11IJ1LLm1JIltlj~1~fhm~~LVitl1J~1ntllJ1ifiJlci Rf~Yil 2v

LLiiI~ 3 1J111J1~U

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 26: 21 .11. 2556 - Ubon Ratchathani University

17

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 27: 21 .11. 2556 - Ubon Ratchathani University

18

11 I I0 JuVI 39 - 311 LLmiddothilililULflmiddotnI1LflLLnmiddotnJfIil~fI1ilV1~nltLVIVlJ1na1lJU fll~V1 1 2

LLat 3 fl1lJi~J

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 28: 21 .11. 2556 - Ubon Ratchathani University

19

323 1)lUU~am1V1flaEll

LflEJ~11tILL1 n111Lfl11~1)mfiEJULViEJU~a)1n1~E1E1ULfl1111LI1ILLnlllmnU~1U)mm ~

V111Lflij (Library) LL~LijEll)1nL t11LLnlllvn mlJ n1~1LfImiddotn~1rUU1fllaU11~UEI-1E1-1fitl1~nElUVl11 LflijVIn1Ua111~LVIEJnl~LViEJlI LliU 3-Viny-l2-dithiocyclohex-S-ene Allyl sufi de 13shy

BenzenedithioL 2-VinyL-l3-dithiane 2-VinyL-4H-l3-dithiin 34-0ihydro-3-vinyL-l2shy

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 29: 21 .11. 2556 - Ubon Ratchathani University

20

dithiin 3-vinyt-[4H]-12-dithiin DiaUyl disulphide Methyl allyl disulfide U~ Methyl

allyl trisutfide LUUI1U ~-3l1el~1ltUL1~11UmS~IlVl1UVI~~~1UiellJ~VIiel1hOellJ~1tl1LfIS1t-1 mh-3hn~1lJ 1ij1nlelelelUlf1SlJ1191umlJvh~1ij1nmSVlIl~el~rlhG11lJflflJel~mLl1UlJuti~

~U5lL~U1~l11elelelUlf1SlJ11~umlJlJel~nSLViulJu~m~Ul51ijiiStlLLUU~LVliielUnU~i1UVIti ell I f d ~o I I q 4 ~

tIml1f1LLfIG11lG11 USVl11~fI11lJ~~lJml1f1V1911LLVIUj911 l LLfIlJfI11lJ LL91 n911nUSVl11-3rlU~ 4 If I I dcJq

nLVltllJ tln9l1eltl1LaU LlJelVl1G11lG11UVl11Uel91flflUlJel~rlflL1~1 1274 U1V1 LLfIV1L1~1

tllJ1ru 1446 U1Vi aelmd1CJlJPI~G1Ln~1Utl~ 33 LmuULViuUnUfhpound1Il~1UL~EJ1nU1~Vl11~ ~I tl-l bull f t 4t a q I bull aI

l1nmaV1UlJ~91SIl91fl LU~ VI 36 ~l1U11 f11G1flG11UVI Lfl~1nmLVlCJlJPISG1 Ln~~lJfl1lJ1nn11V1

1~~1nnLVitllJ~91S~91tl 1UlJru~r11pound1flbudlJelmLViulJPI~G1Ln~u~mLVifJlJfi1~u~iir11 t44 14 AJ Q -13tnflLflfJnu LL91LlJell1~1Sru1V1L1~1 1962 U1V1 ~l11J11f11l1f1lJtJjel-3f1tlSnelUVlL1~1UlIel~

41 0 eft t q

mLVlCJlJ~1rlU lijlJfl1iHn11lJtJj mLV1UlJPIG1Ln~ II

Uel n~1n1u1 (I~ mV1flflel~1trr-3G1elllflel~ nU(Iflm~1t~1E1lJvi~Lau Lellutl~ 33 lflfJ II

IijL~u111tl LL1J1JlJ eltlelelelu lf1SlJ11fl1umlJlJeltmL ViUlJ~91S~fltllijiifl11lJfI1U f1a~nUlJelt q 0 bull q

mLV1t1lJfI1l1UlJ1n n11l1eltmLV1fJlJf1S~Ln~ II

n~111Iltl~1tll~11 LW~trrtW~1lJ1Sfl1LfIS1t-1el~tltlSmelUVl1-3 Lflii~U tiUelU 1~ uti~1n 1 1lt4 1 1tlU1J1JlIel~1elelelU~f1lJ1 ~91Um1JY1 LfI~1n(lflmV1fl~eltun~1lJ11f1UUUULfl11UlJnSLVI UlJUflfl

~1u~u41ijiiel-3fltlSnel1JVlantu~111LVlElLVliielunuLLtinmUmlJ1ru~LL9Inl1tnu VIielel1~n~l1 lI111f111lJUflnl1tlleltlaquofla1UlItHeltRtl1nel1JLflmumLViEllJLLlflriu411uLuuanmuLill11lIelt

= ~1E1rluqUU l

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 30: 21 .11. 2556 - Ubon Ratchathani University

d UVIVI 4

1IJUVIr~~mi11(i~~UU1vm~t~1LLUf1~lJfim~Ll1rJlIv11rJ~lIf1a L~ f1V1 ~trum~ Lvm b~1I~1f1 ~

-~ ell I c q oCtc ~ ~ 0 ~

f11~~1U~1lJVlIJi)m bVI CllJ ~1eurolm~ f11~1~f1~lJm~LVIrJlIVl1rJ~1I f1tlL~ f1V1~eurolIJ f1 a bb~~ f11 ~~1LblJf1VlIJi) ~

m~bl1CJlIv11mVIfli1fl PCA LvmtifJ~~eurolUaIJeurol~tJeurol~bijIJLijeuroli~eurolf1~lJmLl1CllJ

41 UU1Vlv1uJ~~niiLanBi1ml ~tUU~lIf1ilLaf1V1~euroli1f1a~~timilJrIeurol~LflIJLfleuroli (Sensor array) Lf1eurol~~1~iVl~eurol~1LbIJf1f1~IJ ~ ~

LVlCJ~~Yl~1~ru1~1f1ItJLbUUrI~N~eurolUaIJeurol~rIeurol~LflIJLfleuroliVIf1~1tIJmilll11uln1t~1tVl~1V1j~ 1IJ ~

~1IJ1itJdLi1tlf11i1f1aLanVI~tli1mlIJf11I~~1~in~IJtJtl~m~LVirJlIi1VlViIJm)f1lfl~ (Sn02) 7 ~1 ~ ffiVlLlJlJfl1111-$1Ln1~gjtln~IJtJeurol~a1II~LVlaLL~f1nIi1~tiIJl~LLri MQ-2 MQ-3 MQ-6 MQ135

(Hanwei Inc China) TGS2602 TGS2611 LLa~ TGS2620 (Fi~aro Inc Japan) ~~fhfl1111 bull 11 bull bull bull 0

~1IJVI1IJrItl~LflIJL fltl ILVI~1IJ~~LuaVIJLLua~ ~u~111fl1111LrI1IrIIJtIB~ f1111mtla1~VIVI1f11I1 iIiI~LLa iI~

1IJ~1~1~~ 41 ~~UUvttmlJf11IVIiI~tl~dtJI~neurolU1tJr1ahIJ1~~I1i1LLabIJ1LfII1l1~~r~LLail~ tIJ~tJ~ 41

Sensor Array

oXoXoXoXO

Chamber -IIo Signal

conditioning -tilgt

AID

Converter

Computer

- Recording -+

-Analysis

- Display

Sensor Applications

MQ-2 H2 LPG Methane CO Alcohol Smoke Propane

MQ-3 Alcohol Benzene Hexane LPG CO

MQ-6 G H2 Methane CO Alcohol

MQ-135 CO2 NH3 NOxbull Alcohol Benzene Smoke

TGS2602 CH3 Ethanol NH3 Hydro~en sulphide

TGS2611 Methane 1so-butane Hydrogen Ethanol

TGS2620 Ethanol Hydrogen Iso-butane CO Methane

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 31: 21 .11. 2556 - Ubon Ratchathani University

22

1U~UU11lQl1Il~qJqJ1rutgtleJU~UeJjlleJj LllULlleJ~LtJUf11 LL~j~U l1f1Nl~LLtil LL~J~U ~J~J ~tl~ 42

Vout

RL

Buffered

3NO _--4----lt1--0

l1f1~tl~ 42 i1qJqJ1ru~11l1~fieJLL~j~U1vh~h~tgtlf1R~mJflJ111~hUvnUl~~1l RL (VRJ ~J flJ1111I~U5lleJj VRL nULL~j~Ull1Lr1 (Vegt ~atJUltltgtl111~lIm~ 41

VRL = RL Vc ( RL + Rs) (41)

11ltt~ Rs fieJm111~1UVl1Umtt1UlleurolJLllULlleuroli LL RL fieurolRl111~1UVl1Ul~~Il~I9ieurol1Ulj~~ 1U~UU1IlQi)1IlqJqJ1 ru9lmJ~Ueuroljlleurolj LllULlleuroliLtJUf11LL~j~U~1mji)~LLtil LL~J~U

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 32: 21 .11. 2556 - Ubon Ratchathani University

23

1tJn1iVlVl(ltl-JlVl11~iUUtJVlLVltliiWlJlJLUers (Cham ber) ~1V11UUiilmbVitllJ6i1l thntlU~Ul1mLthJtIfI~fnlG1LvHJiilJnVln11l 015 m m1 015 m LL(I ~l 010 m LVltlii

LI1IlULl1Iltli~Vltl~~~11~1UUU vrl~tJ~ 44 ~qjqj1ruvtt~l1mlli1VllQmbtJ(IlbUU~qjqpru~~~t1(1 LVltl NO card (USB6009 National Instrument Inc) ~liifn1lJ(IbBtlVl 14 UVl -iitllJ(Ilt1mnU

II II

LL(IbLGlVllt-l(lUUfltllJ~1b(ltliVln1U1Vi(l(ltlVlli1lb1(11 15 U1Vi ~lfl1UfllJmivi1l1U~1tlLtJiLbmlJ~ ~ilJU1UU LabView

l1flULV1f1JflmitJilJ1(1t-1(1~qjqJ1ruLL(Imi1LfIi1~V11lGli1~ (MATLAB) lQnil1lJ11-ii

1U miVl1f1 ru~ntfruLUYnlJtllt-l(l~t1UGIUtIlVl1l1~Vhvtt~l1 flllJflB L~nVlitlJnG1lltllmLVitllJ LLIii II

(I IJVl ri1f1ru~flI~rum~Ulil1lJ11mUmii11 LLUn~U5mbVitllJ~1tlLV1f1Jflmi~Vlfl~lJLVltl1fi Principal component analysis (PCA) mi1l1UlltlliUU1Vl mi~VlLnUlitll(l LL(IiUU

v bull v

tJ i lJ1(1 t-I (IVIlVllJVllvi1miLetllJlii tl LL(I f11U f1lJ~1tlLtJiLbmlJfltllJ~1 L~ tliVi-wWJU1iUL tllUU LtJiLLmlJ LabVIEW

42 fl1iVlVl6itll1Vln~UmLVialJ

~ 421 ijU~tlUn1iVlVl6itll

1 (ItlmtJiitlmb(lUVlmbVitllJ 2 -almbVitllJViUVlLba1il1V1un 1 g

3 ~lri11ULtJJ bbmlJ LabView rl1V1UVlb1(111Un1i1Vl 15U1Yi bb(le)~i1alJ-iitllJ(I II

11t11U1Vi Lvmvi1n1JVlVlGltlUiUU1Vl1~tltl1UGlJl11itllJ~l1Vl1il1UvrlitJ~ 46 II II

4 LhmbVitllJ~UVlbLa11ab-ii11tJ1UiUU1Vl ~1lJ~tJ~ 47

5 uUVimb(lLbGlVllri1LLHvlUl1m1lUL1ItliUULtJibbmlJ LabView

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 33: 21 .11. 2556 - Ubon Ratchathani University

24

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 34: 21 .11. 2556 - Ubon Ratchathani University

25

422 ~am-SVlail4 J tV I O t 1 Gill d

-sllVI 4 7 LLWl~(Jl1ilV1~~(lJle1JlJEl~(Je~mIijLlijlJLlije-SVl~ 7 Jl1JlElmllJ(JEl~mLVIVlIfI~

LnI ~L~lJ11LlijlJLlijeiLL()a~1iifrl1l1hllJm-sJle1JlJe~~en~lJ(JEl~mLVivlI()1~nlJ a1V1~1Jni1l LjlJLjeiVilPm1JlJe~()ElnalJ(Jil~mLViull1~fie TGS2602 MQ-135 LLa MQ-2 fl111th~1J WEl JJ ~ cJ t tV ~ cJ_ El1~e~~1nJl1~1~VI 41 ~~1J11 TGS2602 LL(l MQ-135 lJlJLL(Jlnfl1~~1mlijlJLlijeifl1EllJ VIlIm-s

Jle1JlJe~()eLLellwLiju llJlIwv1 MQ-2 LiJlJ~1L~U1v1Jle1JlJe~()El Propane ~~El1~mh1111 LjlJLjEliv1JlEl1JlJe~() ElLLElllwdlULL(l1n~L~lJ~Jl81JlJe~~8n~lJlI8~mLViu1l1~~

I I2 ----~----~-----r ~ -shy

I

I

05 - - - - ~ - - - - - - - - - - - - - - +- - - - -- - - - - i- - - shyI I I

--- MQ-2 - - - - - MQ-3

MQ-6 bullbullbullbullbull MQ-l35 --TGS2602

TOS261l TGS2620

I

I

Time (sec)

-sllv148 - 410 LLM~a(JlEl1JUEl~(J~HI~lIni5LilnVl)ElUn~laquo~ 7 i1tJiEln~lJ(JEl~mLVitlll

fILnlt ~fl~~fl6LLathlJU fl11Jih~1J lfltlu()ai1Elfh-1~1m)1fl-l1 12 flf~ LiJlJv1V1)11Jnufi11fl111Jh(JEl~m-S1fln~U~1t1~1Jni5LanVl)ElUn~tu~~~1J~an-SV11J~1n

ElWVlfl1J WltlLVlfll i-rif~1W1t1aflEl1JUEl~i1JWVlfhmiddotvr114na1JbEl-1L1fUL1f9i~~11aLatlm) i4~1)w1t1abEl~ElWVlflm~

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 35: 21 .11. 2556 - Ubon Ratchathani University

-----

---

No1

No

2 N

o3

No

4

2

2

2

2

~

15

15

15

~

15

-

2

~

pound+

secta

z-

__

__

_

bull~

2

~ ~

~

~-~~~-r~~A~

~-~~

~ ~

~ gt

0

5 0

5 0

5 0

5

O~ o

200

400

600

800

0 0 20

0 40

0 60

0 80

0 0 0-

-20

0 roo

60

0 80

0 0

0

200

400

600

800

T

ime

(sec

) T

ime

(sec

) T

ime

(sec

) T

ime

(sec

)

No

5 N

o6

No

7 N

o8

2

2 2

2

--shy

~ 1

5

~

~

ii

I L-i

C

-

-

-

-

~--

-

fl

I ~

gt

gt

05

05

05

05 0

----

shy00

O~shy

200

400

600

800

o 20

0 40

0 80

0 80

0 20

0 40

0 60

0 80

0 o

200

400

600

800

T

ime

(sec

) T

ime

(seo

) n

ne (

sec)

T

ime

(sec

)

No

9 N

oIO

N

o1I

N

o12

2

22

shy

2

1

5 ~

~

---~

15 ~

~0

~

-

~-

f-

~~~~~

-

bullmiddot~i

~--

~

Ifl

~

gt~~

~imiddot~

7

-

_ bull

-

i0

~ gt

gtgt

0

50

5 0

50

5

OL

0

0

200

400

800

600

0 20

0 40

0 60

0 80

0 o

200

400

800

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

00

bull II

II

1

4

tI

41

c

t d

Q

I

0 G

I

llJV

l48

Na91f)lIaJfHlJf)~mCJflCJftHCJff)~

Jmn

~m

1V1t

1lJf

f~a

lf

ll

l1~C

Jfl

12 f

lH

MQ

-6

bullbullbullbullbull

MQ

-t35

----

TGS2

602

TG

S26

I 1

TGS2

620

N

0

--

--shy

---

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 36: 21 .11. 2556 - Ubon Ratchathani University

2f

2

2

-

--shy

-

------

1

15

___

-_

15 --~--

--

15

gt

---

shy~

~

~

~G

tE55

cca

-

~~~

lti

_

~~

~

~~ ~

--

-

-~

~

~

-~-~

~

0~

~

~ gt

M

M

M

0

5

0-

0

0 0

a 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

Ti

me

(sec

) Ti

me

(sec

) Ti

me

(sec

)

Tim

e (s

ec)

No

5

No

6 N

o7

No

8

21

2

------

-

11

~~-shy

~ 5~~=-_~_

~ 1

5 ==

_~_

~_~

~

~

II

j O~

r--

-

-

-

-

--

j 0

_

--

~

05

6

gt 0

5

aL

I__

__~

__~--~~--~~

0shy

00

o

200

400

600

800

0 20

0 40

0 60

0 80

0 0

200

400

600

800

0 20

0 40

0 60

0 80

0

Tim

e (s

ec)

Tim

e (s

ec)

Tim

e (s

ec)

T

ime

(sec

)

No

9

No

IO

No

1

No

12

2

2

2 2

_

tampamp

-shy --_1

IIfIII

15

15

15

~

II

~I_illiil_middot_middot

-

tshy~

~

~

-

----

iimiddot

middot~middotmiddot

middot-

shy6

~

gt

j gt

~rmiddot7b-Et

05

05

0

5

0-middot

0

o

200

400

600

800

0 20

0 40

0 60

0 80

0 01

20

0 40

0 60

0 80

0 0

200

400

600

800

T

ime

(sec

) T

ime

(se

c)

Tim

e (s

ec)

Tim

e (s

ec)

14

tII-

cv

4

tI c

v ~

r~1Jt1 4

9 ~~9

16i

HYn

)-3

11

6-3

fl1O

JflO

JfUamp

0Jf6i

Ltlfl1i1Plm~lt1tJ1JfJ91iPl91fl1Pl0Jf1

12 f

lH

---M

Q-2

M

Q-3

M

Q-6

bullbullbullbullbull

MQ

-13

5

-T

OS

26

02

TO

S261

1 T

0526

20

N

-J

No

1

No2

N

o3

No

4

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 37: 21 .11. 2556 - Ubon Ratchathani University

~ 0

5

_

No1

N

o2

No

3 N

o4

2 2

2 2

15

15

15

~

gt

gt

SV

_

~1

P

_

_

f ~~

II~

~~~

~

~-~

-~~

~~

-~~f

~

i JI

r~

~

~~h

~~n~

=

t i

1-

-=-=

---

~__ ~L-~~_=r_~~_-

gt

gt

gt

gt

u u

u u

o 0

0

0

o ~ ~ ~ ~

0 ~ ~ ~ ~

0 ~

a ~ ~

0 ~ ~ ~ ~

~~

~~

~~

~~

No

5

No

6 N

o7

No

8

2f

2

~21

t

~ _

__

ebullbulln

I ~

lJ)~-

b

tl

-

15~

~

----

--~

----

shy~

~

-

=

-= --

=~

19

S

--

=

---~~

-

-

II

~ o~

=

~ 0

5 ~

0

r-t-=t~

-

--

---M

Q-2

o~ 20

0 40

0 80

0 silo

0~

zO

o 400

aO

o 80

0 0~

~

400

aOo

aOo

0 0 ~

a 60

0 80

0 ~~

r TI

me

(sec

) TI

me

(sec

) TI

me

(sec

)

TIm

e (s

ec)

I

MQ-1

35

-T

GS

26

02

N

o9

No1

0 N

o11

N

o12

TG

S261

1

2f

2 2

l l

TGS2

620

~ ~

-

~ __

____

_ _ ~

lJiR

__ ___

___ t __ _

f t

-

t

i gt

gt

gt

gt

u

U

U

M

00

200 ~ ~

800

00

200

400

800 ~

00 -

2ix

400

silo

aOo

00

200

400

600

800

TIm

e (s

ee)

TIm

e (s

ec)

TIm

e (s

ec)

TIm

e (s

ec)

atl

-ri 4

10 r

1Wlf

llJff

Ufl~

1Jfl~

ftllll

fllllf

Ullll

ffl

Ufl11

1~f)n

aYitJ1

Jdllj

jr~

Gl1112

tli~

~

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 38: 21 .11. 2556 - Ubon Ratchathani University

bull bull

bull bull

29

43 mi1Lfti1oM~1UUnvrufimav~thfJLftUft PCAbull LVlflUfl PCA LthJm~utlflU~01n (OrthogonaL transformation) LflilflVIililvi1

m~utlfl~dutliil1nm~GlLnPlVI~eJmmiddotr)VlltlaVinVl1V1UVI~eJeJr1tl~neJtJIijh~~ (Principal

component ) ~LthJBG1~lPieJnu LVltJ~eJr1tl~neJtJIijh~~VI~n~dLL~n (First principal

component PCl ) iljjri1fld11lLLtl~tl~dU (Variance) ~vi~VlufI~dutl~VI~n~dVlltllijri1 m11lLLtl~tl~dUflVlVIiuniJfIltl LVlflilfl PCA ~ilfJ1l1mum~~1uun-UeJ1JmLtJU Unsupervised

LLfI1m um~flVlIJu1VlIJeJueJllflrieJu11tltl~mfltmlPieJ1tlilnm -ueJ~fI~11tl1Lfl~1dfJ~ ~

LVlflilfl PCA ~Ltluri1LL~~Uil1mlil~1VlijeJIL~ULlIeJh~I 7 ~dmil1nm~1Vln~UijeJImLVifJll -WU~fI 12 flfl LVlfJl-U-UeJ1JfI ru nfl1 123456 u1Vi 1Um~1Lfl~1~~1LLun

PCA CSP Row factor scores

1-__---__

-1 -05 o 05 15 PCl

u1(l1LLil~Um~il1nm~1Vln~U(lmLViEJ~LLlJiflufi~1vi1mi~VlmhJ~1EJLflilfl PCA

lVlfJn1V1uVll~-qVlLLi~Um~il1nmi1Vl~n bull QI bull d 4 QI etJ

Sl LLunfl~miW1Vl 1 VI~eJn~LVlfJ~YfUfitl~aan~ bull QI tai df _

S2 UVlunlllm(lfJ11Vl 2 VI~eJmLVlEJllYfuq~iVlIJlf

S3 LLVlUn~lJ~deJ~11 3 VI~eJmLVlEJ1Ju~~hYfumiddot ~ ~ tIflfl1~~Vln~llri1LL~I~U~U1Vivl1 5 6 vl1EJLVlflilfl PCA LLaVllu~tl~ 411 LVlEJ1i

~

eJIrltl~neJUI1~~~ 1 UfI 2 (PCl LLfI PC2Hilfl~eJtJfll~ri1Utl~tli1U 5701 LLfI

2395 1J111Jh~tJ LW11ilYftJm~~mJVftJih~n~1J LLjilL~u11-beJ1JfIijeJLLllflufiLmn~1l 1

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 39: 21 .11. 2556 - Ubon Ratchathani University

30

Q ~ Q ~

m~nvm)unG1

el~l~1~nPIllJ~~uuviwWJIn~UeJI~elln1~n1~tl-rUlh~L~el~~fl11lJfI~1~LfI~elUviLJUfl~~1 nbull elfJJVIllij l1lJ51n1~L~eln1iL11UL11el1~1~U u~~1U~1UlJelln1l1LfIl1~l-1eJlG11lJl~m~elntiL~Y41~

bull I I

ilellJ~~lmlULlel1viijfl~PIeluG1Uellmvh~U ~~fl~1miVlibufl11lJ~11Un1l LnuilellJ~~~~~LL~~~~ I I I

111~~lJellVIbutl~~lJ1~fl~~1C1 ~LJUtl~~L~UalRqJalVI-rUn1~WWJUl~~UUIJUl~~amfl~elUcJltMI

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 40: 21 .11. 2556 - Ubon Ratchathani University

bull bull

J tJVIVI 5

5 1lo11tl

J1lil~EldLi1Ilo11Jelm~1LL1Jfl~1J5m~1VimJ ~1ElJlJ flE L~nmelUm1 1~El1mU1JiJ1Jfl11lJ LLVlmhlelJ~1J5m~IViEllJv1hlJ1J1fl 3 LLVI~J L~lLri REl1JlllJUelEl JfII~lo1~Lfltl RJ1t11~ J

elVl~Vlti Ufil~ elu11JlriJ ~~hrj1J ~1Elmlo1i11mEl~lJ~~h81JLelVl~elLvi1fil~L1J~fl~llo111J1tlU1JiJ1JL~ hm~LiiiEl1JvILi11J1V1~lo1elU LJ1J n~LViEl1Jj11UUVI n1J LLfil~J1fl mVl1elJRtI~flElUVI11Lflii ellfl~1J

m~LVimJJ1flLElelEl1Jlflll1lV1m1Wm~~1mVIflufl GC-MS ~IJl1n~LViEl1JUjfil~~1JQiilaquoVla11JUElJ EllRtI~ flElUt 1Jlo11~LVlEliifl111JLLVlflj11n1J 1~El~1flfJ~mVI~~EllvYlaEllLVIfluflua~It ~Li11J m~LViEl1J~h~1Ju~~ElVl~Vltiiifl111Jtfl~iiVln1J1J1flflhm~LYiEl1JfII~lo1~Lfltl 1Jfl~El1~flfi111~h m~LYiEl1J~1flUVI~ltI~flvlj11n1J~~iia~a11JUEllellRtI~~flElUVI11LfliivluVlflj1ln1JaElVlfl~EllnU

1

a1J1J~11JL~~111 ~JuaVllt ~L~1Jalfl111JLtJ1JL11m1Jm~1u1Jfl~1JQm~LYiEl1JbEl~1Jfl iiLifflVIelilfl~ ~UU1Vln~um~LYiEl1J~1El~1JfliimflVIielilfl~VI~elh~L~1JL~El~vla~11~1J~1JtI~flElU

1

lt1~1Elh~L~1JL~El~vliifl111Jhjela1ij11n1JvYlVl1J~ 7 ~1 U~~u1El1miVlft~ell1~fl~1Jell m~LYiEl1J~Uhfh~L~1JL~El~vlVlElU lo11JElljEllo11t 1Jmi1JuElfilflel~Ela~~iifl111JL1t 1JmiVlElUlo11JElljEl

~ t I ~ I ~ ~ II

fl~1JUEllm~LVIEl1J 1JJ111Jfl 1JN~Vlm~L~1JL~EliVlVlElUa1JEllVlElU81J IJJL1JElIJfl~ytL~1J1Jfl111J ~1V1El

fl~1Jm~LViEl1J11fl1~ih~L~1JL~el~Uil~~1J ufil~LlelhYiEl1JfilvtL~1Vlfli~LYiElli 3 ilVi ~~ 12 fI~I 1J11LfI1tl1~1U1Jfl~1J1fL~ElLVlflilfl PCA ~U11gEl1J~Ell~1el611m~LViEl1JUilViLfiEl1nuiiu1J11u1J

m~~1El~18~1JIi11fl1EllRtI~flElUVI~flmfl~LfiElln1J alwj~t~Ufl1tijEl1Jn1J~Vl111flq1JU11ujn L~1Ju1J11WEllUj~~fl~1J~1JQm~LViEl1JlflEl61rUftL~1J fJfil mVIVifilellQUU51Jfl111Jlo111J1itlt 1Jm

~1u1Jfl~1JQmtLViEl1J~1flfl~1JEllm~LViEl1J~1Eli~UU~1J fliiLii flVIElilfl~~ILtJ1JtUUvI~1ElUfil~ii fl1t ~1El~1LVl1J1~vIJ~~WJ1J1LtJ1JLfI~Elliitlt 1JmLi1J1Jfil fl1a1Jr11LfltJ1jElltI

52 JElLlo11JtlU1Jt

1 mlo1n~a1t 1Jm~LViEl1Jt ~8~1JltltltllLVlfil1~11mi1LfI1~1~1El GC -MS ~tiiEllRtIi~fltlU VI11LfliiU118V11iWtIi1 fl~t 1JlelEltl1JLfli1J11V1Oi1W nntl1f11~1m~8U1YJtUL~Ellfl111J~1nVltlEll 11 1 cO

~umNufil~IUu~1J1N ILfil~lo111J1tlm1LfI1~ellflutnElUVI11Lfl1JElllo11~LVlEl~1fln~LVIEl1J

l~Elt Head space nJ~Li1LtI~Jtlaltl~1J MVlL~U1J1flpoundil~1J 2 m1LfIi1~l1~1El GC-MS -)~1L~lo1~~1m1V1L~11J1nf1JVl1nlo111J1tl~VlVl1~Ui1~UElllo11L~

fIiUm1J

3 Vl1nlo111J1tllo1~111vi1~~L1J~ntll~1J~m~LViEl1JvYltIitLVIfIIlvin~~1~11JJEl1J~iiiflNfh~El~

tI~LVIfIIEl6111J1n LLj n1ivi1 L il1JmEll t vYlL1~1Ufil~IUtIi~lJ1Nj11J11J1J1n ~~ellm ~L~El1U1Yt1Jm~1bu1Jmi1~Eljtllt1

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 41: 21 .11. 2556 - Ubon Ratchathani University

32

4 fhllLllJLllBi~~~~B1~~ihhVlthml1vlJtIl~LVIflLVhrU Vl1n~11J1lWhLijlJn1l~V1~Bhll LllULllBivtwillii1V1thVlJtIl~LVIflL~1JL~1J1~ fi~~Vh1m~4iB~ltl~ n1tlll11J1J1n~J JBn~1nau1bull 1Jn1lPin~n~B1t1~~Vl~1middotHni1JtlfimV1l~Vrh~1~~jll1~nUjll1~n1llLllJLllBitiU~1l

J

BJr1t1l~ntlUVInU~~~Iij~lItl~n~Jm~LViv1J ~~~~1l11t1an1l-W9lJJ1l~UU~iltll~~VI5n11J1n

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 42: 21 .11. 2556 - Ubon Ratchathani University

1 K Tamaki S Sonoki T Tamaki and K Ehara Measurement of odour after in

vitro or in vivo ingestion of raw or heated garlic using electronic nose gas

chromatography and sensory analysis International Journal of Food Science amp

Technology 43 130-139 (2008)

2 T H Yu C M Wu and Y C Liou Volatile compounds from garlic Journal of

Agricultural and Food Chemistry 37 725-730 (1989)

3 H Amagase B L Petesch H Matsuura S Kasuga and Y Itakura Intake of Garlic

and Its Bioactive Components The Journal of Nutrition 131 955S-962S (2001)

4 H Amagase Clarifying the Real Bioactive Constituents of Garlicmiddot The Journal of

Nutrition 136 716S-725S (2006)

5 Vernin G Metzger J Fraisse 0 Scharff and C GC-MS (EI pet NCt) computer

analysis of volatile sulfur compounds in garlic essential oils Application of the mass

fragmentometry SIM technique (Thieme Stuttgart ALLEMAGNE 1986)

6 G Mazza S Ciaravolo G Chiricosta and S Celli Volatile flavour components

from ripening and mature garlic bulbsmiddot Flavour and Fragrance Journal 7 111-116

(1992)

7 S Munoz-Aguirre A Yoshino T Nakamoto and T Moriizumi Odor

approximation of fruit flavors using a QCM odor sensing systemmiddot Sensors and

Actuators B Chemical 123 1101-1106 (2007)

8 T Nakamoto and H Hiramatsu middotStudy of odor recorder for dynamical change of

odor using QCM sensors and neural networkmiddot Sensors and Actuators B Chemical 85

263-269 (2002)

9 T Yamanaka R Matsumoto and T Nakamoto Fundamental study of odor

recorder for multicomponent odor using recipe exploration method based on

singular value decomposition Sensors Journal IEEE 3 468-474 (2003)

10 T Maekawa K Suzuki T Takada T Kobayashi and M Egashira Odor

identification using a Sn02-based sensor arraymiddot Sensors and Actuators B Chemical 80

51-58 (2001)

11 A Szczurek and M Maciejewska Relationship between odour intensity assessed

by human assessor and TGS sensor array responsemiddot Sensors and Actuators B

Chemical 106 13-19 (2005)

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 43: 21 .11. 2556 - Ubon Ratchathani University

34

12 G Echeverria 1 Fuentes 1 Graell I Lara and M L Lopez Aroma volatile

compounds of []Fujj apples in relation to harvest date and cold storage technoLogy

A comparison of two seasonsmiddot Postharvest Biology and Technology 3229-44 (2004)

13 K Almora J A Pino M Hernandez C Duarte 1 GonzaLez and E Roncal

Evaluation of volatiles from ripening papaya (Carica papaya L var Maradol roja)

Food Chemistry 86 127-130 (2004)

14 M Penza G Cassano F Tortorella and G Zaccaria Classification of food

beverages and perfumes by W03 thin-film sensors array and pattern recognition

techniques Sensors and Actuators B ChemicaL 73 76-87 (2001)

15 GC-MS (Gas Chromatography - Mass Spectrometry)

httpwwwgpoorthrdVhtmVgchtmL retrieved on 201210719

16 GIll1f11 GIJ~~el~ n1fJYf1J~~L~ULel VIanfi1UVI1~iUfim~1J1Um~YfGllll1Lelnan~HUGl1JU1n~ II ill III l I bull

11~Gl1~1~fllVIEJ1f11G1lJ1im~LLnVIV 19(1 75-85 (2005)

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 44: 21 .11. 2556 - Ubon Ratchathani University

lUm~VIfl~tJ)Jlmiddotw GC-MS lJlvi1m~VIfl~tJ~L~lJL~lJlfllrrfl~1tJEi1)n~~LiimJ~1n 5 uVl~~tlJn lJlun P1~ampt~LnM 2 uVlci)tl~n L~EJ)1V1li ~1U u~~tJjl~~~~ U~~~LLVlci~tl~n1fl~1 3 ~1tJEi1) ~~ 2

I

flf~L~tJLtJUm~jl~1~ampttJtlfl11lJfln~tJ~UtJ)~~tltl GC-MS IflEJ1ffL~tJUlUm~1fl~~tJGtl1EJ1Uuvl~ 3 1U ciA it i i I I ltl

VIUUtJLL~fl)LEJ~LLfl tJtJtJU ~fl~lJ1 ~jl~LLmlJLtJ ~if ~Jn1~tJ1~tJ~jltJ

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 45: 21 .11. 2556 - Ubon Ratchathani University

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 46: 21 .11. 2556 - Ubon Ratchathani University

38

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 47: 21 .11. 2556 - Ubon Ratchathani University

39

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 48: 21 .11. 2556 - Ubon Ratchathani University

40

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 49: 21 .11. 2556 - Ubon Ratchathani University

41

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 50: 21 .11. 2556 - Ubon Ratchathani University

42

43

44

45

46

47

48

49

50

Administrator
Oval
Page 51: 21 .11. 2556 - Ubon Ratchathani University

43

44

45

46

47

48

49

50

Administrator
Oval
Page 52: 21 .11. 2556 - Ubon Ratchathani University

44

45

46

47

48

49

50

Administrator
Oval
Page 53: 21 .11. 2556 - Ubon Ratchathani University

45

46

47

48

49

50

Administrator
Oval
Page 54: 21 .11. 2556 - Ubon Ratchathani University

46

47

48

49

50

Administrator
Oval
Page 55: 21 .11. 2556 - Ubon Ratchathani University

47

48

49

50

Administrator
Oval
Page 56: 21 .11. 2556 - Ubon Ratchathani University

48

49

50

Administrator
Oval
Page 57: 21 .11. 2556 - Ubon Ratchathani University

49

50

Administrator
Oval
Page 58: 21 .11. 2556 - Ubon Ratchathani University

50

Administrator
Oval