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2018 CBEES-BBS TOKYO, JAPAN CONFERENCE

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2018 CBEES-BBS TOKYO, JAPAN

CONFERENCE ABSTRACT

2018 8th International Conference on Bioscience,

Biochemistry and Bioinformatics (ICBBB 2018)

January 18-20, 2018

Global Front, Surugadai Campus, Meiji University, Tokyo,

Japan

Sponsored by

Published and Indexed by

http://www.icbbb.org/



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Table of Contents

2018 CBEES-BBS Tokyo, Japan Conference Introduction 3

Presentation Instruction 4

Keynote Speaker Introduction 5

Brief Schedule for Conference 18

Detailed Schedule for Conference 20

Parallel Sessions Index 22

Schedule of Sessions 24

Poster Session 79

Conference Venue 91

One Day Visit and Tour 93

Note 94

Feedback Information 97


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2018 CBEES-BBS Tokyo, Japan Conference

Introduction

Welcome to 2018 8th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2018) which is sponsored by Hong Kong Chemical, Biological & Environmental Engineering Society (CBEES) and Biology and Bioinformatics (BBS). ICBBB conference series held annually to provide an interactive forum for presentation and discussion on Bioscience, Biochemistry and Bioinformatics. The conference welcomes participants from all over the world who are interested in developing professional ties to and/or exploring career opportunities in the region. The conference should serve as an ideal forum to establish relationships from within Japan and other regions of the world.

Papers will be published in one of the following conference proceedings or journal:

International Conference Proceedings Series by ACM. Archived in the

ACM Digital Library, and indexed by Ei Compendex and submitted to be

reviewed by Scopus and Thomson Reuters Conference Proceedings Citation

Index (ISI Web of Science).

International Journal of Bioscience, Biochemistry and Bioinformatics

(IJBBB, ISSN: 2010-3638). Included in the Engineering & Technology

Digital Library, and indexed by WorldCat, Google Scholar,Cross ref,

ProQuest.

Conference website and email: http://www.icbbb.org/; [email protected]

http://dl.acm.org/


mailto:[email protected]


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Presentation Instruction

Instruction for Oral Presentation

Devices Provided by the Conference Organizer:

Laptop Computer (MS Windows Operating System with MS PowerPoint and Adobe Acrobat

Reader)

Digital Projectors and Screen

Laser Stick

Materials Provided by the Presenters:

PowerPoint or PDF Files (Files should be copied to the Conference laptop at the beginning of

each Session.)

Duration of each Presentation (Tentatively):

Keynote Speech: 25 Minutes of Presentation and 5 Minutes of Question and Answer

Plenary Speech: 25 Minutes of Presentation and 5 Minutes of Question and Answer

Invited Speech: 15 Minutes of Presentation and 5 Minutes of Question and Answer

Regular Oral Presentation: 12 Minutes of Presentation and 3 Minutes of Question and Answer

Instruction for Poster Presentation

Materials Provided by the Conference Organizer:

The place to put poster

Materials Provided by the Presenters:

Home-made Posters

Maximum poster size is A1

Load Capacity: Holds up to 0.5 kg

Best Presentation Award One Best Oral Presentation will be selected from each presentation session, and the

Certificate for Best Oral Presentation will be awarded at the end of each session on January

18, 2018 and January 19, 2018.

Two Best Poster Presentations will be selected from poster session, and the Certificate for

Best Poster Presentation will be awarded at the dinner banquet on January 19, 2018.

Dress Code Please wear formal clothes or national representative of clothing.


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Keynote Speaker Introduction

Keynote Speaker I

Prof. Kenta Nakai

The University of Tokyo, Japan

Kenta Nakai was born in Osaka, Japan, in 1963. He received the PhD degree on the prediction

of subcellular localization sites of proteins from Kyoto University, Kyoto, Japan, in 1992.

From 1989, he has worked at several institutions, including, Kyoto University, National

Institute of Basic Biology, and Osaka University. From 1999 to 2003, he was an Associate

Professor at the Human Genome Center, the Institute of Medical Science, the University of

Tokyo, Japan. Since 2003, he has been a full Professor at the same institute. He was elected as

the president of the Japanese Society for Bioinformatics in 2006 and 2007. His main research

interest is to develop computational ways for interpreting biological information, especially

that of transcriptional regulation, from genome sequence data. Two of his papers reporting the

development of a knowledge-based prediction system of subcellular localization sites have

been cited more than 1,200 times so far.

Topic: “Analyses of Transcriptional Regulatory Codes through a Variety of NGS Data”

Abstract—Nowadays, it is becoming more and more important to interpret the impact of

differences between individual genomes and/or the genomes of normal and abnormal cells

(e.g., tumor cells). In many cases, however, this is difficult mainly because we do not

understand how the gene regulatory information is encoded in the non-coding regions of the

genome. To overcome this difficulty, so-called next generation sequencers (NGSs) are quite

useful: they are not only useful just for reading DNA sequences per se but also getting a

variety of information, such as the information on gene expression profiles in a single cell

under various conditions/stages. They are also useful for probing the epigenetic status and the

approximate 3D structure of chromatin. In this talk, I will introduce some of our research

activities in this direction. The talk will include (1) our efforts in automatically extracting the

frequent patterns of motif placements in the genome and our future plan of constructing an

encyclopedia of gene regulatory regions; (2) our ongoing project to combine multiple sources

of Hi-C data for understanding the differential gene expression through differential chromatin

loop structure; and (3) our efforts in analyzing single-cell transcriptome data to understand

inter-tissue communication in the mammalian immune system.


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Keynote Speaker II

Prof. Sung Wing Kin, Ken

National University of Singapore, Singapore

Prof. Dr. Wing-Kin Sung received both the B.Sc. and the Ph.D. degree in the Department of

Computer Science from the University of Hong Kong in 1993, 1998, respectively. He is a

professor in the Department of Computer Science, School of Computing, NUS. Also, he is a

senior group leader in Genome Institute of Singapore. He has over 20 years experience in

Algorithm and Bioinformatics research. He also teaches courses on bioinformatics for both

undergraduate and postgraduate. He was conferred the 2003 FIT paper award (Japan), the

2006 National Science Award (Singapore), and the 2008 Young Researcher Award (NUS) for

his research contribution in algorithm and bioinformatics.

Topic: “Fast, Sensitive and Accurate Detection of Virus Integrations in Cancer”

Abstract—The study of virus integrations in human genome is important since virus

integrations were shown to be associated with diseases. In the literature, few methods have

been proposed that predict virus integrations using next generation sequencing datasets.

Although they work, they are slow and are not very sensitive.

This talk introduces a new method BatVI to predict viral integrations. Our method uses a fast

screening method to filter out chimeric reads containing possible viral integrations. Next,

sensitive alignments of these candidate chimeric reads are called by BLAST. Chimeric reads

that are co-localized in the human genome are clustered. Finally, by assembling the chimeric

reads in each cluster, high confident virus integration sites are extracted.

Finally, we applied BatVI to some cancer datasets and we will discuss the findings we have.


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Keynote Speaker III

Prof. Manoj R. Tarambale

Marathwada Mitra Mandal’s College of Engineering, Pune, India

Prof. Manoj R. Tarambale has received Bachelor's Degree (B.E.) in Electrical Engineering

from BVCOE, Pune-43, University of Pune, India, in 1992 and Master of Engineering Degree

(M.E.) in Control System ( specialization in Instrumentation ) from WCOE, Sangli, Shivaji

University, Kolhapur, India in 2002. Currently, he is pursuing his Ph.D. degree from

PACIFIC University, Udaipur, India in the field of Biomedical Engineering. He has one year

industrial experience and twenty one years teaching experience. At present, he is working as a

Head of Electrical Engineering Department of Marathwada Mitra Mandal’s College of

Engineering, University of Pune, Pune-52, India.

He has published fifteen papers in prestigious International Journals and in International

Conferences. He has got “Most Excellent Paper Award” and “Inter Science Scholastic Young

Investigator Award” for his technical papers published. His main research interests are in the

field of Bio-Medical Image Processing, Bio-Medical Instrumentation, Bio-sensors, Green

Environment, Computer Applications in Bio-Medical Analysis, Artificial Intelligence in

Disease Detection and Electrical Engineering. He is giving an important contribution in

implementing various early detection cancer technique projects through Bio-Medical Image

Processing. He has also done two consultation projects for the reputed industries. Prof.

Tarambale is a member of Institute of Engineers (India) and Indian Society for Technical

Education (ISTE).

Topic: “Detection of Medical Disease (Lung Cancer) Using Image Processing Tool–An

Engineering Approach”

Abstract—Today, cancer is one of the most formidable health problem faced by mankind.

This cancer confirmation process is complex, time consuming and costly. It might be possible

during the above process the stage of the cancer may change. Though MRI, CT scan, PET etc.

radiological images helps us to detect lung nodule easily, but because of huge cost involve

still avoided in poor and developing country. Inexpensive method involve detection of cancer

from simple chest X-ray. Abnormal masses seen in the chest X-ray in the form of white spot

/ tumors are analyze through engineering image processing methods, so that in early stage

cancer conformation took place, which reduce the mortality rate. In an engineering approach


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to detect lung cancer various steps involved are data acquisition, image processing,

segmentation, feature extraction and use of artificial intelligence. First digitized images are

obtain by scanning X-ray image by high resolution scanners and adjusted to standard size by

applying size normalization algorithm. The image processing refer to the tasks necessary for

enhancing the quality of acquired digitizes X-ray scanned images. . The quality of the original

image obtained can be improved further by applying histogram equalization algorithm and

segmentation of suspicious region is done by using various edge detection algorithms,

labeling algorithm etc. Various features are extracted on the basis of mathematics using

simple software programming which help to classify suspicious tumor as malignant or benign

using machine learning process or also called it as using Artificial Intelligence. The proposed

system will not replace the doctor’s role in detection of cancer but it will help doctor to take

correct decision in short time with accuracy (It will act as second opinion before conformation

of cancer).


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Keynote Speaker IV

Prof. Satoru Miyano


Satoru Miyano, PhD, is the Director of Human Genome Center, the Institute of Medical

Science, the University of Tokyo. He received the B.S. (1977), M.S. (1979) and PhD (1984),

all in Mathematics from Kyushu University, Japan. He is an ISCB Fellow. His research

mission is to develop "Computational Medical Systems Biology towards Genomic

Personalized Medicine, in particular, cancer research and clinical sequence informatics. He

has been involved as PI with the International Cancer Genome Consortium, the Grant-in-Aid

for Scientific Research on Innovative Areas (MEXT) "Systems Cancer Research in

Neo-dimension", and MEXT Priority Issues on Post-K computer "Integrated Computational

Life Science to Support Personalized and Preventive Medicine". By massive data analysis and

simulation with the supercomputers, his group is developing computational methods to link

differences in our genomes to diseases, drugs, and environmental factors with systems

understanding.

Topic: “Unraveling Cancer Systems Disorders from Big Data by Supercomputers”

Abstract—Cancer is a very complex disease that occurs from accumulation of multiple

genetic and epigenetic changes in individuals who carry different genetic backgrounds and

have suffered from distinct carcinogen exposures. We present our computational methods for

breaking cancer big data by using the supercomputers SHIROKANE at Human Genome

Center of University of Tokyo and K computer at RIKEN Advanced Institute of

Computational Science. The first challenge is unraveling gene networks and their diversity

lying over genetic variations, mutations, environments and diseases from gene expression

profiles of cancer cells. The second part of this lecture gives details of a suite of

bioinformatics tools named Genomon for analyzing cancer genomes and RNA sequencing

data produced by next-generation sequencers. We present some of our recent results on cancer

genomics with Genomon, including a mechanism involved with “how cancer evades immune

systems.


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Keynote Speaker V

Prof. Chan Jin Park

Incheon National University, South Korea

Prof. Chan Jin Park graduated from Korea University, and got Master and PhD degrees in

same university. His major fields of research are the air pollution control, greenhouse gas

technology and odor management technology. His another interests is green growth policy.

He is now full-professor in Incheon National University at Urban and Environmental

Engineering School. He is member of INU ensemble taking part in piano (Chamber orchestra

of his University).

Topic: “Development of Air Quality Management in Urban Cities”

Abstract—As the economic growth increases various air pollutants are generated to

atmosphere gradually until the air pollution become severe especially in urban areas.

Transportation and industrial facilities and power plants are main reasons of urban air

pollution in major cities of asian regions. Trans-boundary air pollutants such as fine

particulates and odors have been especially common challenge to comfortable and healthful

life of human being. In these situations clean air policy using clean fuel and new and

renewable energy are clearly the main solution to make sustainable air quality. In this research

current states of air quality in major cities were analyzed and the various emission sources

and policies of air quality managements were estimated. Effective methods of air quality

control in urban were suggested to make more clean cities in urban areas.


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Keynote Speaker VI

Prof. Harold Yih-Chi Tan

National Taiwan University, Taiwan

Employment History:

2011~, Director, Center for Weather Climate and Disaster Research

2007-2009 Director, Disaster Research Center, National Taiwan University

2003-2009 Director, Hydrotech Research Institute, National Taiwan University

2003/2-2003/5-Visiting Scholar, Civil and Environmental Engineering, UCLA

1994-now Professor, Bioenvironmental Systems Eng., National Taiwan University

Professional Career Highlights:

1. Professor and Director, National Taiwan University

2. Vice Chairman- ICID- Working Grouping of Environmental Impact of Irrigation, Drainage

and Flood Control

3. National Correspondents of the IAHS Association, IUGG- Chinese Taipei Committee

4. Secretary General, ICID-Chinese Taipei Committee

5. Chairman, Taiwan association of Engineering and Environment

Prof. Harold Yih-Chi Tan's researches related to earthquake to analyze the groundwater level

changed with analytical solution and observed data to be verified to published at Water

Resources Research. Furthermore, the national policy of watershed management and Flood

mitigation after the 921 Chi-Chi Earthquake in Taiwan is published on Natural Hazards. His

research has focused on climate change, land subsidence and disasters reduction.

Topic: “University Capacity Building to Help Local Government Setup Resilient Community

in Taiwan”

Abstract—Global warming impacts of climate and environment changes and it increased

frequency of extremely weather. It causes not only high frequency of disaster, but raising the

high intensity of hazards. For examples, Taiwan 921 earthquake in 1999, South Asia tsunami

in 2004, Hurricane Katrina in 2005, United State of Indiana hail stone in 2006, China Sichuan

earthquake in 2008, Typhoon Morakot at Taiwan in 2009, Haiti earthquake in 2010, Tohoku

earthquake and tsunami in 2011, Eastern Australia in 2012, Typhoon Haiyan at Philippines in

2013, and Bosnia, Serbia and Croatia encountered the most serious floods in past 120 years in


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2014. South Asia floods in 2015. Jiuzhaigou earthquake and typhoon Hato damages threated

Macau and Hong Kong in 2017. Hurricane Harvey is a catastrophe in American in 2017. The

local government is concerning more for damages brought by disasters in Taiwan. The

capacity and manpower of local government is not enough to implement full disaster

prevention in communities level without the help from outside resources. Universities of

Taiwan contribute professional technology, manpower and knowledge to support local

government to raise disaster management capacity in three stages, including risks

identification, adaptation and mitigation, and emergency response.


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Plenary Speaker I

Assoc. Prof. Hiroyuki KUDO

Meiji University, Japan

Prof. Dr. Hiroyuki Kudo received both the M.E. and Ph.D. degrees in the Department of

Electronics and Communications from Waseda University in 1999 and 2004, respectively. He

was a research officer of MEMS laboratory, Tokyo Metropolitan Industrial Technology

Research Institute from 2003 to 2007. He worked at Tokyo Medical and Dental University as

an assistant professor from 2005, a junior associate professor from 2007 to 2011 and an

associate professor from 2011 to 2013. Currently, he has been an associate professor of

Department of Electronics at Meiji University. Currently, his research interests include

biomicrosystems based on enzymatic biosensors and immunosensors and for life science

applications.

Topic: “Electrochemical Biosensors for Healthcare IoT”

Abstract—The internet of things (IoT), which is a new paradigm rapidly spreading in highly

developed information society, have been expected to open new window in treatment and

diagnosis in medicine and daily personal healthcare. Particularly, plenty of physical

information (e.g. body weight, temperature, fat rate, etc.) has been already utilized in cloud

services. However, hurdles for using biochemical information in such information services are

still high. One of the most considerable reason is that simplified means for obtaining

biochemical information are not provided. From this point of view, we position that simplified

method to measure or assess biochemical information (e.g. blood content, saliva content, etc.)

as the key technology for providing health big data and healthcare IoT. It is necessary to

realize a series of processes from ‘sampling’ to ‘measurement’ by an operation that anyone

can do with simple and low-cost instruments for the applications in those fields. We have been

developed new biosensors and biomicorsystems for those purposes by combining the

microelectromechanical system (MEMS) techniques and biochnology. In this talk, our recent

status of development and possible applications will be presented.


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Plenary Speaker II

Prof. Keimei Oh

Akita Prefectual University, Japan

Dr. Keimei Oh was born in Shanghai, China. He received B.Sc. in the Department of

Chemistry from Shanghai University and Ph.D. degree from the Graduate School of

Agricultural and Life Sciences, The University of Tokyo in 1997. After working at RIKEN as

a Special Postdoctoral Fellow, he joined the Department of Biotechnology faculty at Akita

Prefectural University in 1999. In 2003, he worked as a visiting scientist at US Department of

Energy, Plant Research Laboratory in Michigan State University. He was appointed as

Associate Professor at Akita Prefectural University in 2007. Currently, he is working in the

field of design and synthesis biological active chemicals targeting plant hormone biosynthesis

and signaling transduction pathways. He received numerous awards including the Society

Award of the Japanese Society for Chemical Regulation of Plants.

Topic: “Chemical Genetics Strategy Identifies Small Molecules Induce Triple Response in

Arabidopsis”

Abstract—To explore small molecules with ethylene like biological activity, we conducted a

triple response based assay system for chemical library screening. Among 9600 compounds,

we found that N-[ (1, 3, 5-trimethyl-1H-pyrazol-4-yl) methyl]-N-methyl-2-

naphthalenesulfonamide (EH-1) displayed promising biological activity on inducing triple

response in Arabidopsis seedlings. Chemical synthesis and SAR analysis of EH-1 analogues

with different substitution on the phenyl ring structure of the sulfonamide group indicated that

3, 4-dichloro-N-methyl-N-(1, 3, 5-trimethyl-1H-pyrazol-4-yl-methyl) benzenesulfonamide

(compound 8) exhibits the most potent biological activity. To determine the action mechanism,

we conducted RNA-Seq analysis of the effect of EH-1 and ACC, the precursor of ethylene

biosynthesis, following the quantitative RT-PCR confirmation. Data obtained from RNA-Seq

analysis indicated that EH-1 and ACC significantly (above 20 fold of control) induced the

expression of 39 and 48 genes, respectively. Among which 5 genes are up-regulated by EH-1

as well as by ACC. We also found 67 and 32 genes are significantly down-regulated,

respectively. Among which 7 genes are in common. For quantitative RT-PCR analysis. 12

up-regulated genes were selected from the data obtained from RNA-Seq analysis. We found a

good correlation of quantitative RT-PCR analysis and RNA-Seq analysis. Based on these

results, we conclude that the action mechanism of EH-1 on inducing triple response in

Arabidopsis is different from that of ACC.


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Plenary Speaker III

Assoc. Prof. Riichi Kajiwara


Riichi Kajiwara is Associate Professor of Department of Electronics and Bioinformatics at

Meiji University. He received a B.Eng. from Tohoku University in 1993, and M.S. and Ph.D.

degree in information science from Tohoku University, in 1995 and 1998. During the Ph.D.

course degree, he was working at Electrotechnical Lab AIST in Tsukuba science city, and

learned about the basis of electrophysiology and the optical imaging technique with the use of

voltage-sensitive dye. From 1998 to 2013 he worked at AIST, eventually as a Senior Research

Scientist. His research interest is the brain network physiology of the mnemonic and

emotional function.

Topic: “Network Properties of Limbic Neurons Revealed by Voltage-Sensitive Dye Imaging”

Abstract—The 'limbic system' is considered as a crucial structure for the neural plasticity

caused by learning and memory behavior. Therefore the neural circuitry of limbic system is

the primary target of functional modifications relating to the higher cognitive dysfunctions

caused by various factors. The in vitro brain slice preparations have been used for the purpose

so often, due to the difficulty in recording neural activities from deep brain structures under in

vivo conditions. Particularly, hippocampal slice preparations are more commonly used to

investigate the plasticity on a cellular / synaptic level. However, the functional connectivity

should also be examined in much larger scales. Here we discuss about this issue by showing

the optical imaging data obtained from two types of ex vivo brain preparations,

cortico-hippocampal brain slices and isolated whole brain preparations. The isolated whole

brain preparation, in which multi-synaptic circuits and the intracellular activity they generate

are well preserved, can be useful to examine such large scale neural circuitry. In the

presentation, we describe about the functional connectivity of various brain regions in the

limbic system using this unique preparation. This experimental approach combines the

advantages of the in vivo experimental condition with those of in vitro slice preparations, i.e.

an intact synaptic network, excellent mechanical stability, and control over the ionic and

biochemical extracellular environment. In particular, it provides easy access to brain areas of

the limbic system and preserved the neuronal network of the entorhinal-hippocampal loop.

Here we present example data obtained from this preparation in combination with optical

imaging of voltage-sensitive dyes.


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Invited Speaker I

Assoc. Prof. Siew Woh Choo

Xi'an Jiatong-Liverpool University, China

Choo completed his PhD study in Genetics from the University of Cambridge and secured a

scholarship from the Singapore government, to pursued MSc at the School of Medicine at the

National University of Singapore. He is currently an Associate Professor at Xi'an

Jiatong-Liverpool University. Choo has 15 years of working experience in research, academia

and private industry in the fields of genetics and bioinformatics, and mastered a number of

high-end core technologies. He has led a team to participate in local and international projects

with a total funding amount of about RMB 10 millions and published more than 53

peer-reviewed articles (1st or corresonding author in at least 42 articles) in prestigious

journals including Nature and Genome Research with >4850 citations. He has set up and led

the International Pangolin Research Consortium that has members from prestigious Yale

University, Peking University, Washington University, St. Petersburg State University and

Smithsonian Conservation Biology Institute. Choo has also actively transformed scientific

research achievements by setting up a high-tech enterprise and won several national awards.

He has been the Chairman and CEO of a biotechnology company, as well as a Senior

Scientist and Consultant at a personal genomics company. He has served on the editorial

board of Scientitic Reports and has been appointed as National Bioinformatics Expert

Appraiser Panel of the National Malaysian Qualifications Agency (MQA). In addition, he

devoted himself to the higher education industry, taught and educated many postgraduates,

postdoc, undergraduates, and research assistants.

Topic: “Pangolin Genomes and the Evolution of Mammalian Scales and Immunity”

Abstract—Pangolins, unique mammals with scales over most of their body, no teeth, poor

vision, and an acute olfactory system, comprise the only placental order (Pholidota) without a

whole-genome map. To investigate pangolin biology and evolution, we developed genome

assemblies of the Malayan (Manis javanica) and Chinese (M. pentadactyla) pangolins.

Strikingly, we found that interferon epsilon (IFNE), exclusively expressed in epithelial cells

and important in skin and mucosal immunity, is pseudogenized in all African and Asian

pangolin species that we examined, perhaps impacting resistance to infection. We propose that

scale development was an innovation that provided protection against injuries or stress and

reduced pangolin vulnerability to infection. Further evidence of specialized adaptations was

evident from positively selected genes involving immunity-related pathways, inflammation,


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energy storage and metabolism, muscular and nervous systems, and scale/hair development.

Olfactory receptor gene families are significantly expanded in pangolins, reflecting their

well-developed olfaction system. This study provides insights into mammalian adaptation and

functional diversification, new research tools and questions, and perhaps a new natural

IFNE-deficient animal model for studying mammalian immunity.


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Brief Schedule for Conference

Day 1

January 18, 2018 (Thursday)

Venue: Lounge in Front of C5

Arrival Registration 09:30~18:25

Afternoon Conference

Venue: Room C5 of Global Front (17th Floor)

13:00~13:05 Opening Remarks (Prof. Tomohiro Araki)

13:05~13:35 Keynote Speech I (Prof. Kenta Nakai)

13:35~14:05 Keynote Speech II (Prof. Sung Wing Kin, Ken)

14:05~14:35 Keynote Speech III (Prof. Manoj R. Tarambale)

Session 1: 14:35~16:20

Venue: Room C5 of Global Front

(17th Floor)

7 presentations-Topic: “Analysis of

Protein Structure and Function”

Session 2: 14:35~16:20


(17th Floor)

7 presentations-Topic: “Gene

Expression and Analysis”


16:20~16:40 Coffee Break & Group Photo Taking

Session 3: 16:40~18:25


(17th Floor)

7 presentations-Topic: “Enzyme

Engineering and Application”

Session 4: 16:40~18:25


(17th Floor)

7 presentations-Topic: “Medical Image

Processing”

Poster Session: 13:00~18:25 Venue: Lounge in Front of C5

Day 2

January 19, 2018 (Friday) 09:30 ~18:50

Morning Conference


09:30~09:35 Opening Remarks (Prof. Sung Wing Kin, Ken)

09:35~10:05 Keynote Speech IV (Prof. Satoru Miyano)

10:05~10:35 Keynote Speech V (Prof. Chan Jin Park)

10:35~11:05 Coffee Break & Group Photo Taking in the Lounge

11:05~11:35 Plenary Speech I (Assoc. Prof. Hiroyuki KUDO)

11:35~11:55 Invited Speech I (Assoc. Prof. Siew Woh Choo)


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Day 2

Lunch 12:00~13:00 Venue: Lounge in Front of C5

Afternoon Conference

Venue: Room C5 of

Global Front (17th

Floor)

Venue: Room C6 of

Global Front (17th

Floor)

Venue: Conference

Room 1 of University

Hall (3rd Floor )

13:00~13:30

Plenary Speech II

(Prof. Keimei Oh)

13:00~13:30

Plenary Speech III

(Assoc. Prof. Riichi

Kajiwara)

13:00~13:30

Keynote Speech VI

(Prof. Harold Yih-Chi

Tan)

Session 5: 13:30~16:00

10 presentations-Topic:

“Molecular Biology and

Bioinformatics”

Session 6: 13:30~16:00


“Biomedical

Engineering”

Session 7: 13:30~16:00


“Microorganism and

Ecosystem”


16:00~16:20 Coffee Break

Session 8: 16:20~18:50


“Gene Expression and

Analysis”

Session 9: 16:20~18:50


“Basic Medicine and

Drug Development”

Session 10: 16:20~18:50


“Environmental Quality

Assessment and

Management”

Dinner 19:00 Venue: Lounge in Front of C5

Day 3

January 20, 2018 (Saturday) 09:30~17:30

One Day Visit and Tour

09:30~10:00 Departure from APA Hotel Kanda Jimbocho Ekihigashi

10:00~11:00 Visit Meiji University Museum

11:00~12:00 Have Lunch in Local Restaurant

12:30~17:30 City Tour

17:30~ Go Back to APA Hotel Kanda Jimbocho Ekihigashi

Tips: Please arrive at the Conference Room 10 minutes before the session begins to upload PPT into

the laptop.


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Detailed Schedule for Conference

January 18, 2018 (Thursday)


09:30~18:25 Arrival and Registration

Afternoon, January 18, 2018 (Thursday)


13:00~13:05

Opening Remarks

Prof. Tomohiro Araki

Tokai University, Japan

13:05~13:35

Keynote Speech I

Prof. Kenta Nakai

University of Tokyo, Japan

Topic: “Analyses of Transcriptional Regulatory Codes through a

Variety of NGS Data”

13:35~14:05

Keynote Speech II



Topic: “Fast, Sensitive and Accurate Detection of Virus Integrations

in Cancer”

14:05~14:35

Keynote Speech VI

Prof. Manoj R. Tarambale

Marathwada Mitra Mandal’s College of Engineering, Pune, India

Topic: “Detection of Medical Disease (Lung Cancer) Using Image

Processing Tool–An Engineering Approach”

Morning, January 19, 2018 (Friday)


09:30~09:35

Opening Remarks



09:35~10:05

Keynote Speech III

Prof. Satoru Miyano


Topic: “Unraveling Cancer Systems Disorders from Big Data by

Supercomputers”


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10:05~10:35

Keynote Speech IV

Prof. Chan Jin Park

Incheon National University, South Korea

Topic: “Development of Air Quality Management in Urban Cities”

11:05~11:35

Plenary Speaker I

Assoc. Prof. Hiroyuki KUDO


Topic: "Electrochemical Biosensors for Healthcare IoT"

11:35~11:55

Invited Speaker

Assoc. Prof. Siew Woh Choo

Xi'an Jiatong-Liverpool University, China

Topic: “Pangolin Genomes and the Evolution of Mammalian Scales

and Immunity”

12:00~13:00 Lunch Time


Afternoon, January 19, 2018 (Friday)


13:00~13:30

Plenary Speaker II

Prof. Keimei Oh

Akita Prefectual University, Japan

Topic: "Chemical Genetics Strategy Identifies Small Molecules Induce

Triple Response in Arabidopsis"


13:00~13:30

Plenary Speaker III

Assoc. Prof. Riichi Kajiwara


Topic: "Network Properties of Limbic Neurons Revealed by

Voltage-Sensitive Dye Imaging"

Venue: Conference Room 1 of University Hall (3rd Floor )

13:00~13:30

Keynote Speech V

Prof. Harold Yih-Chi Tan


Topic: “University Capacity Building to Help Local Government Setup

Resilient Community in Taiwan”

Note: (1) The registration can also be done at any time during the conference.

(2) The organizer doesn’t provide accommodation, and we suggest you make an early reservation.

(3) One Best Oral Presentation will be selected from each oral presentation session, and the Certificate for

Presentation will be awarded at the end of each session on January 18, 2018 and January 19, 2018.

Let’s move to the session!


- 22 -

Parallel Session Index

January 18, 2018

Session Time Venue Session Topic

1

(P-24~P-27)

14:35~16:20 Room C5 of Global

Front (17th Floor)

Analysis of Protein

Structure and Function

B0038, B0078, B0082, B0098, B0020, B0025, B0085

2

(P-28~P-32)

14:35~16:20

Room C6 of Global

Front (17th Floor)

Gene Expression and

Analysis

B0011, B0032, B0059, B0062, B0069, B0074, B0077

3

(P-33~P-37)


Front (17th Floor)

Enzyme Engineering

and Application

B0073, B0010, B0013, B0033, B0052, B0084, B0092

4

(P-38~P-42)


Front (17th Floor)

Medical Image

Processing

B0008, B0039, B0043, B0045, B0046, B0051, B0110

January 19, 2018


5

(P-43~P-48)


Front (17th Floor)

Molecular Biology and

Bioinformatics

B0034, B0107, T1001, B0021, B3013, B0041, B3011, B0104, B0108, T0008


- 23 -


6

(P-49~P-54)


Front (17th Floor)

Biomedical

Engineering

T0005, B0018, B0035, B0044, B3012, T0015, B0040, B0003, B0031, B0093

7

(P-55~P-60)

13:30~16:00 Conference Room 1 of

University Hall (3rd

Floor )

Environmental Quality

Assessment and

Management

T0003, T0006, T0014, T0021, T0011, T0013, T2005, T1002, T0004, T0023

8

(P-61~P-66)


Front (17th Floor)

Gene Expression and

Analysis

B0081, B0086, B0088. B0114, B0076, B0019, B0115, B0036, B0099, B0014

9

(P-67~P-72)


Front (17th Floor)

Basic Medicine and

Drug Development

B0058, B0067, B0075, B0083, B0087, B0096, B0027, B0055, B0071, B0079

10

(P-73~P-78)

16:20~18:50 Conference Room 1 of

University Hall (3rd

Floor )

Microorganism and

Ecosystem

T0010, T3001, T3002, T3003, T3004, T3007, T0016, T4006, T0028, T0009


- 24 -

Session 1

Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,

we strongly suggest that you attend the whole session.


Time: 14:35~16:20


Topic: “Analysis of Protein Structure and Function”

Session Chair: Prof. Kenta Nakai

B0038

Presentation 1

(14:35~14:50)

Coarse-Grained Molecular Dynamics Simulation of Sulerythrin and LARFH

for Producing Protein Nanofibers

Takashi Ozawa, Hironao Yamada, Takeshi Miyakawa, Ryota Morikawa,

Sota Yagi, Satoshi Akanuma, Akihiko Yamagishi and Masako Takasu

Tokyo University of Pharmacy and Life Sciences, Japan

Abstract—Artificial creation of fibers utilizing proteins has been a target of

bionanotechnology. Yagi et al. succeeded in designing artificial protein

fibers using two types of proteins: LARFH and sulerythrin. Binding

interfaces were designed for sulerythrin and LARFH by introducing

mutations, and the fibrous structures were confirmed by atomic force

microscopy. However, branching was observed in the fibrous structure,

possibly because of non-specific interactions between the proteins. In this

study, we analyzed the behavior and binding sites of sulerythrin mutants and

LARFH mutants using coarse-grained molecular dynamics (MD) simulation.

Binding simulations were performed for a system of one sulerythrin and one

LARFH, and also of two sulerythrin molecules and four LARFH molecules.

These results suggested that glutamic acids originally possessed by

sulerythrin contribute to non-specific binding at sites other than the designed

interfaces.

B0078

Presentation 2

(14:50~15:05)

Isolation and Characterization of Collagen from Red Cheek Barb Scale

(Puntius orphoides)

Sawanit Aichayawanich and Thanya Parametthanuwat

King Mongkut’s University of Technology North Bangkok, Thailand

Abstract—This research involved experimental investigation isolation of

some characteristics of collagen from red cheek barb scale which is the


- 25 -

waste from pickled fish factory. Firstly, the effect of decalcification

conditions including HCl solution concentration (0.8, 1.0, 1.2 N) and

decalcification time (4, 6 h) on ash content of the scale was studied. The

result showed that the lowest ash content was found when decalcification at

highest HCl solution concentration and decalcification time. The decalcified

scales which had the lowest ash content were then extracted the collagen

using three interesting methods to receive acid soluble, pepsin soluble, and

acid soluble - heat collagens. Yields of acid soluble, pepsin soluble, and acid

soluble - heat collagens were 0.43, 0.60 and 0.77% (based on dry weight),

respectively. Tmax of acid soluble, pepsin soluble, and acid soluble - heat

collagens were in the range of 29.2-31.7oC. Tmax of acid soluble - heat

collagen was lowest. L*, a*, and b* values of the collagens were slightly

affected by the extracting method. However, sensory evaluation scores of the

collagens were not depended on extracting methods.

B0082

Presentation 3

(15:05~15:20)

Improvement of Template-Based Protein Structure Prediction by using

Chimera Alignment

Shuichiro Makigaki and Takashi Ishida

Tokyo Institute of Technology, Japan

Abstract—The determination of a protein’s structure provides important

information that can be used for various practical applications in the

biological sciences, such as virtual screening, function prediction, etc.

Protein structures can be precisely predicted using template-based modeling

if we can find good template structures from a database. However, such

predictions sometimes fail even if a template with sufficient quality is found

because the sequence alignment used for the modeling is incorrect.

In this paper, we propose a new method for improving sequence alignment

in single-template-based modeling. The sequence alignments used as an

input of template-based modeling are normally generated by homology

search tools, and the alignments vary depending on the search algorithm

used. Each single alignment is often imperfect, but most of them have

suitable parts for template based modeling at different positions. Thus, a

profile of multiple alignments is typically constructed to obtain a consensus

among the alignments by multiple template search tools. Integrated

alignments are generated by random sampling, and the final prediction

model is selected based on model quality assessment scores and the joint

probability of the profile.

We performed evaluation tests using template-based modeling targets in

CASP11 and compared the proposed method to several existing major

alignment algorithms. The results showed that the proposed method could

improve the model accuracy of single-template modeling.

B0098 Development of Ocular Surface Tribometer and Frictional Characteristics of

Human Ocular Surface


- 26 -

Presentation 4

(15:20~15:35)

Sarwo Pranoto, Shingo Okamoto, Ryoichiro Kataoka, Jae Hoon Lee,

Atsushi Shiraishi, Yuri Sakane, Masahiko Yamaguchi and Yuichi Ohashi

Ehime University, Japan

Abstract—The purpose of this research is to determine the frictional

characteristics of human ocular surfaces. An ocular surface tribometer was

developed to measure the normal forces, frictional forces, and velocities of

the probe on human ocular surfaces simultaneously. In this research, the

measurement on six trial subjects was performed with the developed ocular

surface tribometer. In addition, a mathematical model was proposed to

describe the frictional coefficient of the human ocular surface. The frictional

characteristic curves were found by using the computational program

employing BSG-Starcraft of PSO and LSM developed by the authors in the

previous research. It was possible to classify the frictional characteristics of

human ocular surfaces into three types.

B0020

Presentation 5

(15:35~15: 50)

Targeting lncRNA ASBEL with antagoNAT for Breast Cancer Therapy

Yang Xia and Wang Sheng

Beijing University of Technology, China

Abstract—LncRNAs are RNA transcripts longer than 200nt that lack

protein-coding potential. LncRNA ASBEL has been identified as an

anti-sense transcript of BTG3 gene, which encodes an anti-proliferation

protein. Remarkable down-regulation of BTG3 has been reported in

triple-negative breast cancer (TNBC). In the present study, a number of

single-stranded modified antisense DNA oligonucleotides (antagoNAT) were

designed, synthesized and screened for specific lncRNA ASBEL

knockdown. The antagoNAT played a significant tumor suppressive role in

TNBC by effective down-regulating lncRNA ASBEL, which in turn led to

increased BTG3 expression. We also managed the co-delivery of antagoNAT

and curcumin by using hyaluronic acid-chitosan nanoparticles for TNBC

therapy. The obtained data suggest that the nanosystem-based co-delivery of

antagoNAT and curcumin could achieve synergistic effects on tumor

suppression.

B0025

Presentation 6

(15:50~16:05)

Machine Learning in Predicting Hemoglobin Variants

Monalisha Saikia Borah, Bikram Pratim Bhuyan, Mauchumi Saikia Pathak

and P. K. Bhattacharya

Asian Institute of Management & Technology, India

Abstract—Disease diagnosis is of utmost importance in providing proper

medical treatment. Genetic diseases like hemoglobinopathies and

thalassemia needs to be diagnosed accurately and on time. Though Hb

variants is diagnosed using HPLC based hemoglobin typing machine but


- 27 -

still proper interpretation of the data obtained is necessary and this requires

trained professionals. Machine learning helps in interpreting the obtained

data and helps in predicting the type of Hb variants, thus reducing the work

of health professionals. In this study, the prepared data are classified using

the following classifiers namely Logistic Regression, Support Vector

Classifier (SVC), k-Nearest Neighbor (KNN), Gaussian Naïve Bayes,

Perceptron Classifier, Linear SVC, Stochastic Gradient Descent, Decision

Tree, Random Forest and Multi-layer Perceptron. The pre-processing,

visualization and the classification step were implemented using Python 2.7

with an Intel Core i5 computer. The performance of each classifier is then

tested by first creating a confusion matrix. Indices like ‘precision’, ‘recall’

and ‘f1-score’ are used to quantify the quality of each model. KNN,

Decision Tree and Random Forest shows better classification results in

comparison to the other classifiers. With a precision of 93.89%, recall of

92.78% and f1-score of 93.33%, the decision tree and random forest

classifiers proves to be better classifiers in predicting Hb variants with a

higher accuracy rate.

B0085

Presentation 7

(16:05~16:20)

Study of Cytosolic and Mitochondrial H2O2 Production in LPS-Induced

Microglia Cells Using the H2O2-Sensitive Protein HyPer

Minkyoung Kam, Hanseop Kim and Dong-Seok Lee

Kyungpook National University, South Korea

Abstract—Dysregulation of the production of pro-inflammatory mediators

in microglia exacerbates the pathologic process of neurodegenerative

disease. ROS actively affect microglia activation by regulating transcription

factors that control the expression of pro-inflammatory genes. However,

accurate information regarding the function of ROS in different subcellular

organelles has not yet been established. Here, we analyzed the pattern of

cytosolic and mitochondrial H2O2 formation in LPS-activated BV-2

microglia using the H2O2-sensitive protein HyPer targeted to specific

subcellular compartments. Our results show that from an early time,

cytosolic H2O2 started increasing constantly, whereas mitochondrial H2O2

rapidly increased later. In addition, we found that MAPK affected cytosolic

H2O2, but not mitochondrial H2O2. Consequently, our study provides the

basic information about subcellular H2O2 generation in activated microglia,

and a useful tool for investigating molecular targets that can modulate

neuroinflammatory responses.


- 28 -

Session 2




Time: 14:35~16:20


Topic: “Gene Expression and Analysis”

Session Chair: Prof. Sung Wing Kin, Ken

B0011

Presentation 1

(14:35~14:50)

Comparative Genomic Analysis of Cellulosimicrobium Cellulans and

Phylogenetic Species Searching for Cellulosome-Like Multiprotein

Complex-Encoding Genes

Tongyi Dou, Ran Duan, Chenglin Liu and Le Liu

Dalian University of Technology, China

Abstract—Objective: Previously, we have reported the discovery of a

cellulosome-like multiprotein complex produced by the wild type strain

F16 identified as Cellulosimicrobium cellulans and the structure-function

studies revealed by genomic and proteomic techniques. This work aimed at

comparative genomic analyzing of Cellulosimicrobium cellulans and

phylogenetic-related species, the species of family

Promicromonosporaceae and family Cellulomonadeceae, searching for

cellulosome-like multiprotein complex-encoding genes. Methods: Firstly,

comparative genomic alignment study of the 13 phylogenetic species was

performed using CVTree. Secondly, on protein level, 27 protein sequences

previously identified as subunits of the cellulosome-like complex were

searched by Blastp using the 13 protein-sets as local database. Results:

CVTree results revealed that strain F16 is most close to

Cellulosimicrobium funkei JCM 14302, which is in confliction with the

16S rRNA phylogenetic results whereas strain F16 is most close to

Cellulosimicrobium cellulans JCM9965. Since whole genomic

comparative analysis is more reliable to the 16S rRNA results, this result

may overturn previous conclusions. Moreover, according to local Blastp

results, gene M768_06655, M768_10315, and M768_18415 were

identified in consistent with the production of the cellulosome-like

complex. Conclusion: This work provides clues and predictions for

uncovering molecular mechanism of cellulosome-like complex production


- 29 -

by species of family Promicromonosporaceae and family

Cellulomonadeceae.

B0032

Presentation 2

(14:50~15:05)

Simulation Study for Wild Type and C101F Mutant of LIM2 Domain in

FHL1

Hiroki Takeuchi, Hiroki Okajima, Hironao Yamada, Takeshi Miyakawa,

Ryota Morikawa, Masako Takasu and Yukiko Hayashi


Abstract—Myopathy is a rare disease lacking a fundamental therapy.

Several genetic factors are involved in myopathy; those caused by

mutations in FHL1 are rare. We performed molecular dynamics simulation

of the LIM2 domain in FHL1 (four and a half LIM domain protein 1). We

simulated a partial system consisting of only the LIM2 domain for the

wild-type and C101F mutant to confirm the structural stability. We found

that structural changes and fluctuations were larger for the mutant type

than for the wild-type. Therefore, mutant type structures are unstable in

water when the mutations are in residues constituting the zinc finger.

Similar results were observed in the simulation of the LIM1+LIM2

domain.

B0059

Presentation 3

(15:05~15:20)

Alignment of Unaligned Reads for the Detection for Novel Genes Using

the Hanwoo Whole Genome

Kelsey Caetano-Anolles, Kwondo Kim, Woori Kwak, Samsun Sung,

Dajeong Lim, Bong-Hwan Choi and Heebal Kim

Seoul National University, laboratory of bioinformatics and population

genetics, South Korea

Abstract—Hanwoo, also known as Korean native cattle, are a breed of

cattle world renowned for the high percentage of fat marbling in their meat.

This breed has begun to be used as a main source of meat in the past

century; since then, demand for this product in Korea has skyrocketed.

Genomic data gathered from 136 Hanwoo cattle was analyzed using

advanced statistical methods in this study. The primary objective of this

study was to assemble and map unaligned reads in order to identify and

predict novel genes. These genes were cross-checked using a protein

domains identifier. Cross-referencing with the Pfam database identified

associated protein domains covering a total of 178 regions. These domains

were characterized mostly by immune-related domains related to

Immunoglobulin function. Interesting was the expression of the Interferon

group of signaling proteins, which is crucial for immune system response.

Although research into the genetic aspects of and expression of genes

related to immunoglobulin has been widely conducted in humans and mice,

research in this field is lacking when it comes to livestock breeds,


- 30 -

particularly cattle. This kind of information can be used to create synthetic

recombinant species-specific antibodies, which could be used to treat and

prevent infectious diseases.

B0062

Presentation 4

(15:20~15:35)

Regulation of Host Genome Methylation and Expression in HIV Infection

Xi Zeng and Kwok Wing Stephen Tsui

The Chinese University of Hong Kong, Hong Kong

Abstract—It has been shown that boththe methylationand gene

expressionplay rolesin HIV-host interplay.In this study, tofurther

investigatethe relationship between HIV infection andpatterns of

genome-wide methylation and gene expression, we performedMeDIP-seq

and RNA-seqfor five T cell linesand each cell line contained HIV+ and

control samples.Withindifferentially expressed genes (DEGs)between

HIV+ and control samples,the top enriched pathways included “primary

immunodeficiency” and “inflammatory response”. Eighteenup-regulated

and eightdown-regulated DEGswere shared byfourcell lines. Moreover, on

average,1,736differentially methylated regions (DMRs)were found

betweenHIV+ and control samplesfor each cell line. DMRs were

significantly enriched in promoter and exon regions. The top enriched

pathways ofDMR-associated genes were“ubiquitin-dependent protein

catabolic process”and “GTPase activator activity”.Besides, 635 genes were

also involvedin the crosstalkof methylation and expression, out of which

ATP1B1, CAMK2D, GRIN2A, MAPK10, CACNA1C and F2R were shared

by more than two cell lines and enrichedin “cAMP signaling pathway”,

which affectsHIVreplication and infection.This study broadens our

understanding on the mechanism of HIV/AIDSandprovidesa resource

about dynamic changesat thetranscriptome and epigeneticlevelsduringHIV

infection.

B0069

Presentation 5

(15:35~15: 50)

Iterative Scaling Fuzzy Additive Spectral Clustering (is-FADDIS)

Algorithm for Subtypes Cancer Identification using Gene Expression Data

Ito Wasito, Muhammad Faturrachman, Ionia Veritawati, Indra Budi and

Betha Nurinasari

Universitas Indonesia, Indonesia

Abstract—Cancer is one of main non-communicable disease. Analysis of

subtypes cancer identification is important to determine the proper

treatment for each patient. However, cancer data analysis is difficult

problem since it has multiple risk factors genes and clinical condition of

patients. This study aims to identify subtypes cancer using Iterative

Scaling-FADDIS (is-FADDIS) algorithm which extends the FADDIS

algorithm in sparse data problems. In this research, is-FADDIS technique is

applied to microarray data to identify subtypes cancer in two data sets:


- 31 -

Colorectal Carcinoma and B-Cell Lymphoma. Then, the clusters structure

those to be found will be validated based on Correlation Ratios and Global

Silhoutte. For Colorectal Carcinoma cancer, the correlation ratio between

cluster and the existence of tumour will be analyzed. The results of

experiments show that is-FADDIS works well on both data sets. For

Colorectal Carcinoma cancer data, the results show that those clusters to be

found by is-FADDIS has successfully correlated with the existence of

tumour. For B-Lymphoma dataset, is-FADDIS algorithm has successfully

well separated two clusters of cancer subtypes with Global Silhoutte value

0.4035.

B0074

Presentation 6

(15:50~16:05)

Exploring Genetic Variation in 40 Pigmented Rice Varieties via Whole

Genome Sequencing Data for Rice Breeding

Rabiatul Adawiah Zainal Abidin, Zeti Azura Mohamed Hussein and

Norliza Bakar

National University of Malaysia (UKM), Malaysia

Abstract—Single nucleotide polymorphisms (SNPs) provide a valuable

resource in understanding the genetic basis of pigmented rice varieties.

Unfortunately, not much analysis has been done on the SNPs of pigmented

rice varieties. We have performed a comprehensive bioinformatic analysis

on the 40 varieties of pigmented rice whole genome data to identify SNPs

that are involved in the production of flavonoids and other compounds with

antioxidant properties. Out of 2,460,172 high-quality SNPs discovered,

8514 of these SNPs are polymorphic. Gene ontology (GO) enrichment

analysis revealed the classification of these polymorphic SNPs in various

biological processes such as cellular metabolic process, secondary

metabolite biosynthesis, amongst others. In addition, some genic SNPs

were found in genes encoding enzymes of flavonoid biosynthesis in both

black (4119 SNPs) and red (6369 SNPs) rice genomes. From these

combined datasets, 2138 non-synonymous SNPs were identified where this

shows possible mutations in the genes encoding enzymes of flavonoid

biosynthesis; i.e. encoding for anthocyanin in black rice and

proanthocyanidin in red rice. Results of this study can be used as a

resource to be applied in rice breeding program towards the improvement

of its nutritional value.

B0077

Presentation 7

(16:05~16:20)

Selection of the Most Informative Schemes for Multi-Locus Sequence

Typing

Ji-Wei Lin, Yen-Yi Liu and Chih-Chieh Chen

National Sun Yat-sen University, Taiwan

Abstract—To investigate and surveillance outbreak of disease we need

molecular fine subtyping. We present a website tool, PGAdb-builder.


- 32 -

PGAdb-builder can establish a pan-genome allele database (PGAdb) and

whole genome multilocus sequence typing (wgMLST) approach applied to

achieve subtyping layout that can be comparable among laboratories. We

build a PGA database of Salmonella enterica Serovar Typhimurium as a

test for the website. The dendrogram (genetic relatedness tree) shows

epidemiologically well-characterized S.Typhimurium isolates. But large

amount locus of the strain makes the subtyping process time-consuming.

We implement Python program to improve the situation by making use of

some feature selection methods (variance threshold, univariate selection,

recursive feature elimination, principal component analysis). These

methods utilized to reduce the quantity of the locus by removing low

influence or useless ones. Decreased strain locus dataset can create similar

group of outbreak strain compared with the raw dataset result. Employing

the feature selection method can be a more efficient way to reach the same

goal.

Coffee Break & Group Photo Taking

16:20~16:40 Lounge in Front of C5


- 33 -

Session 3 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,

we strongly suggest that you attend the whole session. Afternoon, January 18, 2018 (Thursday)

Time: 16:40~18:25


Topic: “Enzyme Engineering and Application”

Session Chair: To be added

B0073

Presentation 1

(16:40~16:55)

Metabolic Engineering of Extended MEP Pathway for Production of

Cis-Abienol and Isoprene

Egils Stalidzans, Katrina Daila Neiburga, Vitalijs Komasilovs and Agris

Pentjuss

Latvia University of Agriculture, Latvia

Abstract—To utilize the potential of plants as “green factories”, new

regulatory and biosynthesis pathways have to be introduced and correctly

expressed to maximize productivity. Metabolic modeling is applied to test

the opportunities of production of cis-abienol and isoprene in Arabidopsis

thaliana as model plant. Kinetic models of 2-C-methylerythritol 4-phosphate

(MEP) pathway with extensions towards production of 1) isoprene and 2)

cis-abienol are developed and optimised for better yield. Total optimization

potential (TOP) approach is applied to find minimal enzyme set necessary to

reach requested fraction of TOP without full combinatorial search of

adjustable parameter (enzyme concentrations) combinations. Total enzyme

activity constraint is applied to represent the limited capacity of protein

building machinery. Homeostatic constraint is applied to limit the impact of

intermediate metabolite concentration changes on reactions outside the

scope of the model. The initial values of carotenoids, chlorophyll and

plastoquinones fluxes are fixed during optimization. COPASI software and

COPASI wrapper SpaceScanner are used. Several metabolic designs (sets of

enzyme concentration changes) are proposed for experimental

implementation.

B0010

Presentation 2

Structure-Activity Relationship Study of Pentacyclic Triterpenoids as Potent

Inhibitors against Human Carboxylesterase 1

Yangliu Xia, Tongyi Dou, Lili Jiang, Yong Liu and Guangbo Ge


- 34 -

(16:55~17:10)

Dalian University of Technology, China

Abstract—Human carboxylesterase 1 (hCE1), one of the most important

serine hydrolases distributed in liver and adipocytes, plays key roles in

endobiotic homeostasis and xenobiotic metabolism. In the present study, a

series of pentacyclic triterpenoids were selected as the model ligands and in

vitro inhibitory activities against hCE1 were tested. By using the

three-dimensional quantitative structure-activity relationships (3D-QSAR)

method of comparative molecular field analysis (CoMFA), the structure and

activity relationship of these compounds was studied. The 3D-QSAR modes

displayed good correlation between the variations on percent inhibition and

the steric-electrostatic properties, with the cross-validation coefficient,

non-cross validation coefficient and external verification of the complex

correlation coefficient of 0.512, 1, and 0.72, respectively. Converting the

C-3 keto of the pentacyclic ring to hydroxyl, and C-17 hydroxymethyl to

carboxyl group, led to a significant enhancement of the inhibitory effect on

hCE1. These exciting findings inspired us to design and synthesize the more

potent compound (IC50 0.015 μM) as a novel inhibitor against hCE1, which

was 897-fold more potent than the parent compound betulin. All these

findings will be implicated for the design and further optimization of potent

hCE1 inhibitors, which could serve as a promising tool for exploring drug

candidates for clinical applications in selectively modulating

hCE1-associated toxicities.

B0013

Presentation 3

(17:10~17:25)

NEDD4 is Down-Regulated by OTU-T Deubiquitinating Enzyme

Jun-Hyeok Park, Soo-Yeon Kim, Da-Hye Lee and Kwang-Hyun Baek

CHA University, Republic of Korea

Abstract—Deubiquitination is an inverse process of ubiquitination mediated

by deubiquitinating enzymes (DUBs). DUBs have the ability to deconjugate

ubiquitin molecules from ubiquitinated substrates and stabilize them. Thus,

DUBs have been considered to be pivotal mediators in diverse cellular

systems including various signal transduction pathways such as cell death

and cell proliferation. OTU-T, one of DUBs, which is a member of the

ovarian-tumor protease (OTU) family, and is found that it interacts with

Neural precursor cell Expressed, Developmentally Down-regulated 4

(NEDD4) through bioinformatics tools. OTU-T is one of the ubiquitin E3

ligases, which is involved in the regulation of diverse processes including

insulin-like growth factor signaling and neural architecture. In this study, we

demonstrated that OTU-T binds to NEDD4, and NEDD4 interacts with

OTU-T regulating cell proliferation. And knock-out and overexpression

studies were performed to investigate the effect of OTU-T on cell

proliferation. Immunoblotting and colony forming assays revealed that the

level of NEDD4 protein was decreased with a gradual increase of OTU-T

expression, while the rate of cell proliferation was decreased in a


- 35 -

dose-dependent manner of OTU-T expression. Important directions for

future studies will include the analysis of mechanisms that regulate cell

proliferation mediated by Hippo signaling pathway.

B0033

Presentation 4

(17:25~17:40)

Differentially expressed deubiquitinating enzymes in the presence of toxic

environmental chemicals

Youngjae Hwang, Da-Hye Lee, Jihye Choi and Kwang-Hyun Baek

CHA University, Republic of Korea

Abstract—Premature ovarian failure (POF) is the loss of function for the

ovaries in women less than 40 years of age. Diseased POF ovaries cannot

produce a normal amount of estrogen and cannot ovulate periodically,

resulting in infertility. However, the cause of POF is not fully understood

yet. To investigate the differential expression of deubiquitinating enzyme

(DUB) genes in patients with POF, we developed a POF model by treating

with toxic environmental factors including formaldehyde, which might

cause POF in human. Using this model, a multiplex RT-PCR assay was

performed to identify putative biomarkers by comparing expression levels of

DUB genes encoding cysteine proteases. Interestingly, the assay revealed

that the level of expression for POF-DUB1, POF-DUB2 and POF-DUB3

was increased. The difference in the expression level of several proteolytic

enzyme genes was confirmed by repeated experiments, and the possibility of

using them as biomarkers to diagnose POF was demonstrated.

B0052

Presentation 5

(17:40~17:55)

Uses of Copra Waste and Wheat Bran for Cellulase Production by

Trichoderma reesei in Solid State Fermentation

Teerin Chysirichote, Lexpet Nareerat, Kaisaard Sawwanit and Chysirichote

Teerin

King Mongkut’s Institute of Technology Ladkrabang, Thailand

Abstract—The potential use of copra waste from coconut pressing process

for cellulase production by solid-state fermentation (SSF) was studied in this

research. Trichoderma reesei TISTR3080 was cultivated at 30°C for 7 days

on the copra waste (CW) with and without supplementary of wheat bran

(WB) as a nitrogen source. The results showed that adding WB increased

both the fungal growth and productions of cellulase including activities of

total cellulase, CMCase and β-glucosidase. The fungal growth which was

indirectly measured by glucosamine measurement increased from 0.33 d-1

in the CW without WB to 1.1 d-1 in the CW added WB at the ratio of 3:3.

Moreover, adding WB to CW at the ratio of 3:3 accelerated the activity of

β-glucosidase from 12.6 to 16.8 U/ g dry substrate at 1 d. However,

supplementing CW with WB at the ratio of 3:2 enhanced the total cellulase

activity and CMCase activity from 0.31 to 5.23 FPU/ g dry substrate at 2 d

and 0.42 to 5.18 U/ g dry substrate at 6 d, respectively.


- 36 -

B0084

Presentation 6

(17:55~18:10)

The Epithelial-Mesenchymal Transition in Colon Cancer is Promoted by

Peroxiredoxin 5

Jin-Woo Yoo, Kyung-Min Kim and Dong-Seok Lee

Kyungpook National University, Republic of Korea

Abstract—Globally, colorectal cancer (CRC) is common cause of

cancer-related deaths. The high mortality rate of patients with colon cancer

is due to cancer cell invasion and metastasis. Initiation of the

epithelial-to-mesenchymal transition (EMT) is essential for the

tumorigenesis. Peroxiredoinxs (PRX1-6) have been reported to be

overexpressed in various tumor tissues, and involved to be responsible for

tumor progression. However, the exact role of PRX5 in colon cancer

remains to be investigated enhancing proliferation and promoting EMT

properties. In this study, we constructed stably overexpressing PRX5 and

suppressed PRX5 expression in CRC cells. Our results revealed that PRX5

overexpression significantly enhanced CRC cell proliferation, migration,

and invasion. On the other hand, PRX5 suppression markedly inhibited these

EMT properties. PRX5 was also demonstrated to regulate the expression of

two hallmark EMT proteins, E-cadherin and Vimentin, and the

EMT-inducing transcription factors, Snail and Slug. Moreover, in the

xenograft mouse model, showed that PRX5 overexpression enhances tumor

growth of CRC cells. Thus, our findings first provide evidence in CRC that

PRX5 promotes EMT properties by inducing the expression of

EMT-inducing transcription factors. Therefore, PRX5 can be used as a

predictive biomarker and serves as a putative therapeutic target for the

development of clinical treatments for human CRC.

B0092

Presentation 7

(18:10~18:25)

In silico identification of novel kinase inhibitors by targeting PIM1 from

natural products database

Tian Yu, Youling Sun, Xinjie Lian, Feng Liu and Zheng Shi

Chengdu University, China

Abstract—Kinase provides a rich and diverse source of potential targets for

blocking tumor growth and survival. And, protein kinase inhibitors have

made a substantial beneficial impact on the therapeutic care of cancer

patients. The PIM family of serine/threonine kinases is named due to their

mode of discovery as proviral common integration sites in moloney murine

leukemia virus-induced lymphomas. PIM kinases are involved in a number

of signaling pathways which plays a vital role in cancer cells. They act in

downstream effector functions as inhibitors of apoptosis and as positive

regulators of G1-S phase progression through the cell cycle. Since virtual

screening has become an integral part of the drug discovery process, it is of

great significant to identify novel PIM inhibitors by structure-based virtual

screening. In this study, we carried out a series of elegant bioinformatics


- 37 -

approaches, such as virtual screening, molecular dynamics simulations to

identify PIM inhibitors from Food and Drug Administration

(FDA)-approved small molecule drugs. Following the 5 ns MD simulations,

Dofetilide (ZINC49583080) were recognized as “new use” FDA-approved

drug for PIM harboring cancer patients. Together, our findings may provide

successful application of virtual screening studies in the lead discovery

process, and suggest that our discovered small molecules could be effective

PIM inhibitor candidates for further study.


- 38 -


we strongly suggest that you attend the whole session. Afternoon, January 18, 2018 (Thursday)

Time: 16:40~18:25


Topic: “Medical Image Processing”

Session Chair: Prof. Manoj R. Tarambale

B0008

Presentation 1

(16:40~16:55)

Automatic Liver Segmentation in Abdomen CT Images using SLIC and

AdaBoost Algorithms

Mucahid Barstugan, Rahime Ceylan, Mesut Sivri and Hasan Erdogan

Selcuk University, Turkey

Abstract—This study is an implementation of liver segmentation on

abdomen CT images. The liver organ was segmented by using SLIC

super-pixel and AdaBoost algorithms. Firstly, the images were clustered by

SLIC super-pixel algorithm. Then, the liver was segmented by AdaBoost

classifier. The segmentation process was done automatically. The automatic

segmentation is based on the classification of overlapping patches of the

image. The results of automatic segmentation and manual segmentation

were compared and the efficiency of the method was observed. The best

Dice rate was obtained as 92.13% and the best Jaccard rate was obtained as

85.8% on 16 abdomen CT images.

B0039

Presentation 2

(16:55~17:10)

Efficient Vertebra Segmentation Using Graph Cuts

Jin-Yuan Hsue, Chan-Pang Kuok, Ting-Li Shen, Bing-Feng Huang and

Yung-Nien Sun

National Cheng Kung University, Taiwan

Abstract—Spine surgeries are highly dangerous when applied for treating

spine diseases or injuries. In order to achieve less tissue damage and faster

recovery, minimally invasive surgery is desirable. Before operation, the

surgeon has to precisely plan the procedures based on the

three-dimensional(3D) virtual spine model which is usually segmented from

the patient’s computed tomography(CT) images. The segmentation accuracy

of conventional methods relies on the initial contour and much efforts are


- 39 -

spent for initialization. We propose a graph cuts based segmentation method

to segment the vertebra from CT images and build a 3D model for the

surgical planning. In this study, a multi-region graph cuts algorithm with

simple initialization is applied to segment the vertebra in the axial view CT

images. The segmentation results are used to build the 3D spine model. The

average dice coefficient is 0.96. We compare the segmentation performance

with the Chan-Vese level set method. We find the proposed result is better

fitted to the ground truth and needs less computational time. Consequently,

we proposed an efficient and automatic segmentation method to segment

vertebra and build a 3D spine model for pre-operational planning.

B0043

Presentation 3

(17:10~17:25)

Quantitative Assessment of Skeletal Muscle Contusion Recovery using

Ultrasound Statistical Parameter and Image Texture Feature Extraction

Zih-You Yu, Hsiou-Yi Lin and Shyh-Hau Wang


Abstract—Contusion is a common injury and that may be further diagnosed

clinically via the evaluation of medical images by experienced experts. In

the present study, efforts were made to extensively assess the recovery of

contusion tissue using ultrasound quantitative parameters and texture

feature. The experiments were performed from SD rats where the contusion

were achieved by an impact of 600g mass and 30cm height falling from a

lab-made impact platform. The integrated backscatter (IB), statistical

Nakagami parameter, and texture features extraction using gray-level

co-occurrence matrix (GLCM) were calculated from the acquired ultrasound

radio-frequency signals and B-mode images with a 30 MHz transducer. IB

and Nakagami parameter of muscle tissue before the contusion were

estimated to be above 20 dB and 0.4, respectively. After muscle contusion,

hematoma formation in the middle of necrotic muscle fibers led both IB and

Nakagami parameter to be lower than 20dB and 0.4 respectively. Twenty

days after muscle contusion, the quantitative parameters tended to recover to

those of healthy muscles. The texture features of bruise muscle using GLCM

was also found to be less echogenicity and evenly distributed, which were

postulated owing to and the hematoma. This study validated that

high-frequency ultrasound and related parameters as well as GLCM texture

features may be a feasible diagnostic means for quantitatively characterize

the degree and recovery of muscle contusion.

B0045

Presentation 4

(17:25~17:40)

Detection of Kinetic Trajectory of Tissues in the Wrist Using Ultrasound

Images for Assessing the Degree of Carpal Tunnel Syndrome

Cheng-Che Tsai, Chia-Huang Lin and Shyh-Hau Wang


Abstract—The ultrasonic measurement of the kinetic trajectory of median


- 40 -

nerve (MN) has been found to be potentially capable of diagnosing carpal

tunnel syndrome (CTS). Previous studies were performed from healthy

subjects by using normalized cross correlation (NCC) tracking technique,

and which demonstrated to be a feasible technique but required tremendous

computational time. In this study, efforts were made to apply optical flow

tracking (OFT) and adaptive Bayesian speckle tracking (ABST) techniques

to detect the movement of MN for assessing the degree of CTS. The

measurements were performed using a commercial ultrasound scanner in

healthy subjects and CTS patients. Results showed that three techniques are

capable of detecting the movement of MN and OFT takes the least

computational time. The kinetic trajectory of healthy subjects is different

from that of CTS patients. The maximum displacement of MN for subjects is

larger than those of patients. Furthermore, the movements of tendons

surrounding MN were found to provide another information for assessing

the degree of CTS. The distance between the tendon and the membrane of

subjects tended to be larger than those of patients. Current studies

demonstrate that ultrasonic detection of movements of tissues may be a

feasible means for CTS diagnostics noninvasively.

B0046

Presentation 5

(17:40~17:55)

Classification of Ultrasonic Nakagami Parameter by AI-based Method for

Identifying Liver Fibrosis

Ting-An Wu, Bo-Yen Huang and Shyh-Hau Wang


Abstract—Clinically, the diagnostics of liver fibrosis using biopsy and

histopathological slice may achieve the highest accuracy; however, the

invasive nature of these means is still with the potential of liver

inflammation and psychological discomfort for patients. The non-invasive

ultrasound (US) images were acquired from the liver of SD rats, for which

the fibrosis was induced by 𝐶𝐶𝑙4. An AI-based Convolutional Neural

Network (CNN) model was proposed to analyze US image for classifying

the degree of normal, fibrosis and cirrhosis of the liver. The feature images

in the CNN model were extracted in the convolution layers, and then

reduced into low-order feature images in the pooling layers. Finally, the

fully-connected (FC) layers compute the weight of the feature images.

Nakagami parameter (𝑚), an US statistical parameter for quantitatively

analyzing scatterer properties, was also added into the CNN model to

increase the accuracy of assessment. Results demonstrated that 𝑚 parameter

of control group was 0.58±0.03, mild group was 0.78±0.02, and severe

group was 0.83±0.02. Previous researches demonstrated that both transfer

learning and data augmentation could increase the categories and the amount

of data. Following the inclusion of these methods, the classification accuracy

of current model could approach to 90% or better.

B0051 RFID Based Tracking and Positioning for Hospital Pneumatic Delivery


- 41 -

Presentation 6

(17:55~18:10)

System

Kuo-Sheng Cheng and Yi-Syong Chen


Abstract—Although pneumatic tube delivery system has been widely used

since the late 19th century, some important issues associated with its

functions are not thoroughly investigated. One of the most common

problems is how to quickly track the carrier getting stuck in complicated

tubing system, especially in hospital. The exhaustive search for locating its

position is usually very time consuming. This paper proposes the use of

radio frequency identification (RFID) technology to track and locate the

carrier’s position during delivery. The average speed for carrier during

delivery is about 6-10 m/sec. In this high-speed applications, UHF based

RFID is considered as a suitable solution. The tags are placed on the outside

of the transport tubing system and the RFID transponder is attached fixedly

in the carrier. Each tag is coded for position or sender/receiver. The proposed

system inside the carrier contains mobile power supply, RFID reader, PCB

antenna, and Raspberry pi 3 controller with Wi-Fi module. The EPC code is

employed for designating the tube position, sending/receiving unit, and

goods inside the carrier. The programming language for software

development is Python. When the carrier is sent from the sender, the

controller will check the code as a start. Then, the series of positions’ tag are

read and stored in the memory. Afterwards, the code for receiver is check.

Once it is confirmed, the route for carrier is sent to the PC system through

Wi-Fi. If it is stuck in the tube, then the location may be alarmed for

repairmen. The proposed system is demonstrated to be feasible and helpful

in hospital application.

B0110

Presentation 7

(18:10~18:25)

Discriminative Local Feature Fusion for Ear Recognition Problem

Ibrahim Omara, Xiaoming Li, Gang Xiao, Khan Adil, and Wangmeng Zuo

Harbin Institute of Technology, China

Abstract—Ear recognition problem is known as selecting whether two ear

images belong to the same person or not, this consider as a challenge due to

variation in lighting, background, pose, scale, and occlusion. This paper

presents an improvement method for unconstrained ear recognition problem

based on local feature fusion, and further analyzes the performance and

efficiency of discriminative local feature fusion for aligned and non-aligned

ear images. Firstly, local discriminative features such as LPQ, HOG, LBP,

POEM, BSIF and Gabor features are extracted from the ear images. Then,

Discriminant Correlation Analysis (DCA) is exploited for fusion and

reduction dimension. Finally, support vector machine (SVM) is adopted for

classification. Experiments are conducted on popular ear databases, USTB I,

USTB II, and IIT Delhi II. Furthermore, we report an encouraging result on


- 42 -

a difficult and challenging ear database called annotated web ear (AWE) that

is collected from the wild. The experimental results show superior of

proposed approach that can achieve a high performance for non-aligned

images (AWE and USTB II datasets), on the other hand, unique local

features can achieve promising recognition rates for aligned images, USTB I

and IIT Delhi II datasets.


- 43 -


we strongly suggest that you attend the whole session. Afternoon, January 19, 2018 (Friday)

Time: 13:30-16:00


Topic: “Molecular Biology and Bioinformatics”

Session Chair: Prof. Satoru Miyano

B0034

Presentation 1

(13:30~13:45)

Structural Analysis of Metal Binding Peptides using Molecular Dynamics

Mao Watabe, Hironao Yamada, Takeshi Miyakawa, Ryota Morikawa,

Masako Takasu, Tatsuya Uchida and Akihiko Yamagishi


Abstract—Proteins that specifically bind metals have been the target of the

research for developing new organic-inorganic hybrid materials. Some

amino acid sequences that bind metal have been reported, and the

structures of proteins and peptides are considered responsible for binding

to metal. The purpose of this study is to identify molecular structures

responsible for binding metals. We performed molecular dynamics

simulations and structural analyses of metal-binding peptides. The most

frequently appearing structure of each peptide was identified. Combined

with the previous experimental results, peptides with a stable, specific bent

structure were suggested to have strong binding abilities. Peptides with a

different bent structure have been suggested to be responsible for weak

binding ability.

B0107

Presentation 2

(13:45~14:00)

mRNA Structure in Translation Regulation and its Selective Shaping on

Sequence

Yuming Xu and Wanjun Gu

Southeast University, China

Abstract—Many studies have suggested various functional roles

potentially played by the secondary structure of mRNA in gene regulation.

One of previous works demonstrated reduced stability of the mRNA

structure near the start codon in almost all cellular life and this reduction is

probably selected for efficient translation initiation. We utilized similar


- 44 -

methods to systematically further our investigation in structures around

two other regions. In animals, but not in plants, we discovered a universal

trend of increased mRNA stability near the 5’cap in mRNAs regulated by

miRNA. GC content of 5’UTR, 5’UTR length and number of miRNA

target sites may influence local structure near the 5’cap, which is likely

selected to facilitate miRNA-mediated translation regulation. Another

consistent pattern--decreased structure just before the stop codon followed

by a strong signal of increased stability--was found near the translation

termination site in many species, except in yeast and worms. This pattern is

selected for efficient bounding of termination release factors and prevents

erroneous stop codon readthrough. However, among simple organisms

readthrough heterogeneity may enhance phenotypic diversity and facilitate

their adaptation to changing environments.

T1001

Presentation 3

(14:00~14:15)

Effect of Water Hyacinth on the Mechanical, Morphological and Physical

Properties of Baked Starch Foams

Sukaree Chaireh and Kaewta Kaewtatip

Prince of Songkla University, Thailand

Abstract—The objective of this work was to study the effects of water

hyacinth (WH) on the properties of baked starch foam and compare them

with the effects of commercial cellulose (CC). The effect of the both fillers

on the properties of baked starch foams including density, morphology and

impact strength was investigated. FTIR analysis revealed the structure of

WH to be composed of cellulose, hemicelluloses and lignin, whereas CC

contains only cellulose. It was found that the impact strength of starch/WH

composite foam was greater than the impact strength of both starch/CC

composite foam and starch foam without filler by about 58% and 43%,

respectively. This increase may be the result of a more efficient transfer of

stress from the starch matrix to the filler due to the strong interaction

between the hydroxyl groups in the structure of the three-dimensional

network of lignin in WH and starch. Moreover, the starch/WH composite

foam showed the best morphology of small and uniform cell size, probably

because the WH can increase the steam bubbles in the starch batter. It can

be concluded that water hyacinth is a novel filler that plays an active role

in improving the properties of baked starch foam.

B0021

Presentation 4

(14:15~14:30)

Enumerating Chemical Mono-Block 3-Augmented Trees with Two

Junctions

Yuui Tamura, Aleksandar Shurbevski, Hiroshi Nagamochi and Tatsuya

Akutsu

Kyoto University, Department of Applied Mathematics and Physics, Japan

Abstract—Enumerating chemical graphs with given constraints on their


- 45 -

topological structure is a fundamental problem in chemoinformatics and

bioinformatics, with a wide range of applications, including structure

determination and designing novel chemical compounds. We specify the

constraint on the structure by the frequencies of all paths up to a fixed

length in the graph. Given upper and lower bounds, we consider the

problem of enumerating chemical graphs whose path frequency satisfies

one withing the given bounds. A kaugmented tree is a connected

multigraph where the number of pairs of adjacent vertices minus that of

vertices is k −1, and a monoblock k-augmented tree is a k-augmented tree

that contains one biconnected component. The biconnected component of a

monoblock 3-augmented tree with two junctions contains two vertices

joined by four internally disjoint paths. We design an algorithm for

enumerating all mono-block 3-augmented trees with two junctions that

satisfy given upper and lower bounds on path frequencies. Experimental

results indicate that our algorithm performs favorably when compared to a

state-of-the-art production program, and we obtain large numbers of

structures with up to 35 atoms excluding hydrogen, which has not been

possible with existing methods.

B3013

Presentation 5

(14:30~14:45)

Peroxiredoxin 3 Promotes Cell Motility via Modulation of the Extracellular

Matrix

Jayantha Gunaratne, Pei-Jou Chua and Boon-Huat Bay

Institute of Molecular & Cell Biology, A*STAR, Singapore

Abstract— Peroxiredoxin 3 (PRDX3), is a thiol-specific antioxidant

enzyme that reduce various cellular peroxides substrates using a

cysteine-containing active site. It is overexpressed in most cancers,

however, its exact roles and mechanisms in carcinogenesis have yet to be

fully elucidated. In this study, we employed biochemical, cell biological

and proteomics approaches to understand its roles in breast cancer. First,

we performed immunohistochemistry in breast cancer tissues that showed

that high expression of PRDX3 in breast tumor tissues compared to

adjacent normal tissues. In addition, we observed that high expression of

PRDX3 was also associated with lymph node metastasis. Biochemical

assays of PRDX3 knockdown and overexpressed MDA-MB-231 breast

cancer cell line showed that PRDX3 is positively correlated with cell

migration and invasion. To delineate PRDX3 roles in these processes, we

carried out quantitative proteomics analysis of PRDX3 knockdown and

wildtype MDA-MB-231 cells that allowed identification of 159

differentially expressed proteins (DEPs) in PRDX3 knockdown cells.

Pathway and protein network analysis of these DEPs revealed enrichment

of proteins associated with mainly extracellular remodeling processes.

Ongoing validation experiments pertaining to these processes together with

PRDX3 interactome analysis will shed light onto specific mechanisms of

PRDX3 in breast cancer cell migration and invasion.


- 46 -

B0041

Presentation 6

(14:45~15:00)

Effect of Various Composition of Medium on Antioxidants and

Antioxidation Capacity in Sweet Potato (Ipomoea Batatas Lam.)

Suspension Culture

Guam-Jie Cheng and Chien-Kuo Wang

Asia University, Taiwan

Abstract—In this research, by changing growth hormone, cytokines,

sucrose, iron ions and organic nitrogen concentration, the changes of

phenol, flavonoid contents and the anti-oxidation ability in sweet potato

cell cultures were studied. Results show that increasing content of sucrose

and KNO3 in the medium resulted in significant improvement of total

phenolic compounds and flavonoids content as well as cell growth.

Changing the NH4+

and FeSO4 concentration in B5 medium could cause

the same effects except increase in cell growth. A suspension cell culture

has the highest total phenolic compound contents, 9.5mg, and flavonoid

contents, 132.7 ug, respectively; and it has the highest anti-oxidation

ability in this research, too.

A potato suspension cell culture with high phenolic compound contents,

high flavonoid contents and high anti-oxidation ability was obtained, it

could be used in further study in the potato breeding.

B3011

Presentation 7

(15:00~15:15)

Fetal Movement Detection During Pregnancy with the Electrical

Impedance Tomography

Konki Sravan Kumar, Anil Kumar Khambampati, You Jung Han, Sung

Yob Kim and Kyung Youn Kim

Jeju National University, South Korea

Abstract—An appropriate and inexpensive, continuous, non- invasive

methods are required to detect the fetal movements, which are important

and fundamental clinically significant parameters of fetal growth and

well-being during pregnancy. This work introduces dynamic electrical

impedance tomography imaging technique for continuous monitoring of

fetus movements. Dynamic electrical impedance tomography (EIT) is

continuous monitoring, a non-invasive, non-ionizing and low cost imaging

modality in which the electrical conductivity or resistivity in the interior of

a pregnant abdomen can be reconstructed by injects sinusoidal electrical

currents to the surface of the pregnant abdomen and collects the voltage

measurements. In this paper EIT forward problem solved by using

boundary element method with (BEM) with complete electrode model

(CEM) and applied smooth variable structure filter (SVSF) method as an

inverse solver to estimate the conductivity distribution inside the pregnant

woman's abdomen. Numerical simulations were performed in Matlab

software and simulation results are accomplished to verify the performance

of the dynamic EIT system.


- 47 -

B0104

Presentation 8

(15:15~15:30)

Soy Peptone as a Non-Animal Origin N-Source in Salmonella Typhi

Culture Medium For Production of Vi Polysaccharide as a Material of

Typhoid Vaccine

Triana Rahmila, Wardono Niloperbowo and Erman Tritama

School of Life Sciences and Technology – ITB, Indonesia

Abstract—Currently, typhoid vaccine is produced by using a cultivation

media that uses materials originating from animal (animal origin) as one of

its main components. Thus, there is a need for a research about the

production of typhoid vaccine that uses a cultivation media that is free of

animal origin (non-animal origin). This study was done to demonstrate the

correlations between the concentration of capsular polysaccharide Vi, the

antigen source for the production of typhoid vaccine, with the bacterial

strains, medium contents and cultivation methods. The strain that was used

in this study was production strain, Ty2 and patient isolate. One of the

chosen strain candidates was used to compare between the mediums that

used animal origin and non-animal origin in a batch process and fed-batch

process. It was found that the concentration of Vi increased by threefold

when the medium was made using soy peptone (non-animal origin),

instead of casamino acid in a fed-batch process. It was also found that the

optimum glucose concentration of the feed media is 50% of the original

media that was use in the batch phase. The parameters that was used to

analyse this study was optical density at 600nm (OD600), pH, glucose

concentration, and the concentration of Vi that was measured by ELISA.

This study has shown that soy peptone can replace casamino acid as the

component of the medium in the production of capsular polysaccharide Vi.

B0108

Presentation 9

(15:35~15:45)

Reconstruction of Secondary Metabolism of Cordyceps Militaris

Warasinee Mujchariyakul, Chayapat Wizaza and Wanwipa Vongsangnak

Kasetsart University, Thailand

Abstract—Cordyceps militaris is one of entomopathogenic fungi which is

widely used for biometabolites production e.g., cordycepin, adenosine,

cordycepic acid, ergosterol, and polysaccharide. So far, a genome sequence

of C. militaris is available; however there remains largely unknown on

secondary metabolism. Therefore, this study aimed to annotate enzyme

functions for further reconstructing secondary metabolism of C. militaris

using bioinformatics approach. To carry out, we initially used 9,651

predicted protein sequences of C. militaris for enzyme annotation using

EnzDP algorithm. Upon EnzDP together with KEGG database, the results

afterwards showed that 15 secondary metabolic pathways were identified.

Of these pathways, puromycin biosynthesis, indole diterpene alkaloid

biosynthesis, betalain biosynthesis and carbapenem biosynthesis were

considered as the primary results for secondary metabolism reconstruction


- 48 -

of C. militaris. The findings provide a basic knowledge in secondary

metabolite biosynthesis in C. militaris.

T0008

Presentation 10

(15:45~16:00)

Synchronization between Respiration and Mastication Functional

Coordination of Respiratory and Digestive Systems

Yutaka Yoshida, Emi Yuda, and Junichiro Hayano

Nagoya City University, Japan

Abstract—The purpose of our study is to evaluate the relationship between

respiratory period and chewing period. In 10 healthy peoples (6 males and

4 females, age 26 ± 7 years), EMG of masticatory muscles and respiratory

curve during chewing of gum were measured and synchronicity between

respiration and chewing were analyzed using the phase of chewing

occurring within respiratory period. Results, the coherence between the

two signals at the peak frequency of respiration was 0.06 ± 0.03 (mean ±

SD). In all subjects, the relationship between respiration period and

chewing period was random and was not correlated. In this experiment,

chewing period wasn’t controlled. Therefore, it is considered that

respiration may have been synchronized if the period of chewing was

controlled.


- 49 -



Time: 13:30-16:00


Topic: “Biomedical Engineering”

Session Chair: To be added

T0005

Presentation 1

(13:30~13:45)

Assessment of Flow Mediated Dilation by Pulse Wave Conduction Delay

Junichiro Hayano, Yutaka Yoshida, and Emi Yuda


Abstract—This study aimed at developing a new method for assessing

flow mediated dilation (FMD) by bilateral differences in pulse conduction

time. Delay in pulse conduction time (PCD) that accompanied FMD

induced by forearm reactive hyperemia with 5-min avascularization was

evaluated. PCD increased with FMD but the correlation coefficient

between PCD and FMD was 0.33. PCD may have been influenced by

unmeasured factors other than FMD.

B0018

Presentation 2

(13:45~14:00)

Added Force-Based Methods Using Load Cells to Evaluate Walking and

Tumbling with a Japanese Walking Support System

Shinji Kawakura and Kiyoshi Fujiwara

National Institute of Advanced Industrial Science and Technology (AIST),

Japan

Abstract—We have conducted sequential studies to develop promising

systems that address nursing challenges, and have developed applied

sensing systems to solve the difficulties faced to advanced support systems

for physically challenged and elderly people. In this study, our main aim

was to present the utilities of the immediate and distant measurement

systems by presenting qualitative and quantitative timeline data from one

user of a typical Japanese walking support System. We suggested a

physical analysis system using four bar-shaped metal load cells, Arduino

microcomputers, and set specialized integrating and amplifying circuit

boards on the arm rest to obtain data of the added weight of users on the


- 50 -

front point of the machine. Additionally, we developed a physical analysis

system, which uses two video cameras to obtain visual data of a subject

simulating using programs written in Visual C++ and OpenCV packages,

including the libraries and header files. Finally, we presented the multiple

output values of the physical measurements obtained from various

viewpoints, and examined the characters of timeline data and their

kinematic parameters. We believe these mixed methodologies could be of

practical use in providing alternative supports and useful directions for

developers and care managers to assess and treat users’ conditions.

B0035

Presentation 3

(14:00~14:15)

Optimal-channel Selection Algorithms in Mental Tasks based

Brain-computer Interface

Han Sun, Bruce Gluckman, Xiong Zhang, Yu Zhang, Xuefei Zhong and

Zhaowen Fan


Abstract—Brain computer interface (BCI) for healthy people is a growing

field. Minimizing the number of electroencephalography (EEG) channels is

a key technological advantage for the application of BCI, which would

make the system more mobile, easier to setup and long-time use in the real

life. In this paper, to decrease the number of channels, multi-channel

common spatial pattern (MCSP) algorithm is used to extract the features

with two mental tasks (i.e., mental arithmetic and spatial imagery), and

support vector machine (SVM) is used to classify the tasks performed. In

detail, the separability value of each individual channel is computed based

on between/within-group variance and a modified entropy criterion to

evaluate its contribution to classification performance. The optimal

channels are chosen based on the separability ranking. The performance of

proposed methods is compared with recursive channel elimination and

genetic algorithm. The results demonstrate that the EEG signals have

different trends between the two mental tasks with highest brain activity in

left central-parietal and parietal lobes, and the separability values allow

reduction of number of electrodes from 15 to 4 and 10 while the

classification accuracy reaches 80% and 90% respectively. Therefore, the

optimal-channel algorithms can reduce the number of channels and

improve the performance of the mental tasks based BCI.

B0044

Presentation 4

(14:15~14:30)

Development of Portable and Wireless Closed-loop Systems for

Responsive Neurostimulation and Neurofeedback Training in Animals

Sheng-Fu Liang, Yu-Hsuan Shih, Wen-Hsiu Yeh and Fu-Zen Shaw


Abstract—Advances in electronic and information technologies support the

development of many new diagnostics and treatments for healthcare. A

closed-loop platform that can perform on-line event detection and real-time


- 51 -

stimulation or feedback such as responsive neurostimulators and

neurofeedback training (NFT) systems will perform alternative treatments

in addition to pharmacological therapy. A closed-loop seizure controller

that can perform on-line seizure detection and real-time electrical

stimulation for seizure elimination is developed and applied to Long-Evans

rats with spontaneous absence epilepsy. The seizure detection accuracy is

greater than 92% and the averaged seizure detection latency is less than 1

s. 90% of the absence seizures can be suppressed by a 0.5-s 800 Hz

electrical stimulation on zona incerta. The wireless EEG monitoring

module and a LabVIEW interface are also integrated with a Skinner box to

perform neurofeedback training. If the power of alpha rhythm of an animal

is sustained and higher than the threshold, the food is given as a reward.

This NFT system can successfully train the Sprague Dawley rats to have a

progressive significant increase in the alpha amplitude and total alpha

duration in the 12 training sessions. The developed systems can also be

applied to various researches in bioelectronics, medical informatics and

neuroscience in the future.

B3012

Presentation 5

(14:30~14:45)

Effective Decolorization of Malachite Green Using Kocuria Marina

DAGII and Its Toxicological Study

Tonmoy Ray, Daiji Brahma, Ruchira Mitra and Debjani Dutta

National Institute of Technology, Durgapur, India

Abstract—Malachite Green (MG) is a highly toxic synthetic dye which is a

major component of textile industry. It alone accounts for the two-third of

the total production of the wastewater. Biological decolorization of dye is

highly effective and a promising method due to its low cost and

environmental friendly approach. The present study involves efficient

detoxification of Malachite Green by the bacterium Kocuria marina DAG

II through batch process. It was observed that more than 99 % dye removal

was achieved within 8 hours at 28°C with an initial MG concentration of

13mg/L, initial pH of 7.2 and an inoculum size of 1.2% (v/v). Microbial

toxicity test was conducted against Bradyrhizobium sp. using Agar well

diffusion method. It was inferred that the treated effluent had no zone of

inhibition but on contrary the untreated MG solution exhibited 35 ±5 mm

inhibition zone in agar plates. Additionally the toxicity of the treated dye

solution was checked on the seeds of Vigna radiata (Indian mung bean), a

major cultivated Indian pulse crop. Though the phytotoxicity results

revealed 100% germination in distilled water (positive control), untreated

and treated dye solution, however the root and the shoot length were

negligible in case of untreated dye solution. In contrast, the root and shoot

length for treated MG solution was similar to the distilled water. Thus,

the toxicological studies indicated that the treated dye solution was

appreciably less toxic compared to untreated MG solution.


- 52 -

T0015

Presentation 6

(14:45~15:00)

Wii Balanced Board-based Utilization for The Human Body Center of

Pressure for the Falling Detection via Multivariate Empirical Mode

Decomposition and Multivariate Multi-Scale Entropy

Jiann-Shing Shieh, Ting-Hui Li, Chih-Dao Chen, and Maysam F. Abbod

Yuan Ze University, Taiwan

Abstract—Center of pressure (COP) evaluation is one of important

parameters in the fall detection algorithm for the elder people. The Wii

balance board (WBB) (Nintendo Co., Ltd., Redmond, WA 98052),

originally an accessory of a game controller for the Wii video game

console, is a potential device that can be utilized for the human balance

evaluation system. Initially, this study evaluates the WBB signal in

comparison to the AMTI (Advanced Mechanical Technology Inc., Newton,

MA, USA) as the reference. Initially, the experiment is conducted for three

different conditions; open eye, close eye and one-foot stand for five

volunteers. The mean of correlation coefficient between WBB and AMTI

is over 0.98 on X-direction and over 0.99 on Y-direction. The multivariate

multi-scale entropy (MMSE) and multivariate empirical mode

decomposition (MEMD) are used for the signal feature evaluations. The

results show that complexity indexes (CI) from the MSE-based algorithms

have significant results only between young and elderly groups and young

and elderly-fall groups. However, there are no significant differences on

between elderly and elderly-fall groups. In conclusion, this study shows

that the WBB can be applied as the balance-measuring device. Meanwhile,

further investigations need to be conducted for the advanced algorithms to

fall detection for the elderly people.

B0040

Presentation 7

(15:00~15:15)

A Wearable Wireless fNIRS System

Yu Zhang, Xiong Zhang, Han Sun, Xuefei Zhong and Zhaowen Fan


Abstract—Neurovascular coupling mechanisms in functional brain activity

has always been important areas of brain science. Functional near-infrared

spectroscopy (fNIRS) has been utilized in the recent decades for examining

functional brain activity by measuring the changes in the concentrations of

oxygenated and deoxygenated hemoglobin. fNIRS is a noninvasive

detecting technology, it has many characteristics such as high measurement

speed, simple operation, fNIRS represents a good compromise in terms of

spatial and temporal resolution, and so it’s becoming more and more

widely used. But recent fNIRS still non-wearable and participants are

nonmoving.

In this work, we design a low-cost, low power, wearable and wireless

fNIRS system. This system has two wavelengths light of 760nm and

850nm, and transfer data via Bluetooth. We design and make a plastic


- 53 -

housing for fix the optical fiber by 3D printer. The battery can last several

hours. In an experiment, this system can detect change of Hb and HbO2’s

concentration.

B0003

Presentation 8

(15:15~15:30)

Preparation of an Electrically Conductive Graphene Oxide/Chitosan

Scaffold for Cardiac Tissue Engineering

Lili Jiang, Daoyu Chen, Zhongmin Zhang, Yangliu Xia, Hongyu Xue and

Yong Liu

Dalian University of Technology Panjin, China

Abstract—Cardiac tissue engineering is thought to be of great importance

for therapeutic and pharmaceutical applications. The scaffolds that can

provide electrical conductivity and structural organization would be highly

beneficial for cardiac tissue engineering. Here, we developed conductive

scaffolds with electrical conductivity and porous structure composed of

chitosan (CS) blending with graphene oxide (GO) for cardiac tissue

engineering. Our results showed that the swelling, porosity, and conductive

properties of GO/CS scaffolds could be modulated via adjusting the ratio

of graphene oxide to chitosan. More importantly, GO/CS scaffolds had

swelling ratio ranging from 23.20 to 27.38 (1000%) and their conductivity

(≈0.7×10-5 S·cm -1) fell in the range of reported conductivities for native

cardiac tissue. Furthermore, we assessed their biological activity by

seeding H9C2 cells in GO/CS scaffolds. Our data showed that these

GO/CS scaffolds exhibited good cell viability, promoted cell attachment

and intercellular network formation, upregulated expression of the cardiac

specific gene and protein involved in muscle conduction of electrical

signals (connexin-43). Overall, it is concluded that the GO/CS scaffolds

promoted the properties of cardiac tissue constructs, demonstrated an ideal

scaffold for cardiac tissue engineering.

B0031

Presentation 9

(15:35~15:45)

Measurement and Evaluation of the Bioelectrical Impedance of a Rubber

Humanoid Phantom Wrapped with Gel Sheets

Shinji Kawakura, Yoshihiro Nakabo and Kiyoshi Fujiwara

National Institute of Advanced Industrial Science and Technology (AIST),

Japan

Abstract—This study aimed to estimate the impedance values of an

originally made, artificial rubber phantom attached with one elastic gel

sheet using Physion MD (Doctor of Medicine). The electromagnetic

characteristics of the material were similar to human body. In previous

studies, specially designed bioelectrical impedance acquisition systems and

MRI (magnetic resonance imaging) methods were used to independently

and repetitively measure bioelectrical impedance and serial cross-sectional

images of the trunk, forearms, upper arms, lower legs, and thighs of


- 54 -

healthy adult men. Bioelectrical impedance was significantly correlated to

muscle and fat volumes measured by MRI. Based on this relationship, the

estimated values of muscle and fat could also be calculated from the

bioelectrical impedance. However, past methodologies have various

limitations. In this current, subsequent research, only impedance values

were measured, while varying superficial variables (e.g., attaching gel

sheets, changing electrode types, and changing the insertion depth of

needle-shaped electrodes) This present study indicates that bioelectrical

impedance analysis for this rubber phantom including the version with

specific gel sheets attached and the version containing normal saline

solution is a promising physical simulator, and may be useful for the

prediction of not only virtual muscle volume, however, also diverse inner

body conditions.

B0093

Presentation 10

(15:45~16:00)

Preparation of Calcium Phosphate Biocomposite and the Study of Bone

Formation in Mice

Lijia Cheng, Jianhong Yang, Tao Chen, Xinrong Xie and Zheng Shi


Abstract—Objective: To prepare a type of thermos-sensitive

hydrogel/tricalcium phosphate (TSH/TCP) biocomposite, and investigate

its osteogenic ability. Methods: The TSH was dissolved at 4℃, then the

TCP powder was added into TSH at a ratio of 24/76, and the

24TSH/76TCP biocomposite was prepared above 27 ℃ . Next, the

composite materials, hydroxyapatite (HA) and β-tricalcium phosphate

(β-TCP) were implanted into the thigh muscles of BALB/c mice; twelve

weeks after the operation, three types of materials were harvested, fixed

and paraffin embedded, then the sections were performed histological

staining to observe the new bone formation. Results: At 12 weeks, a large

number of new bone tissues were detected in both TSH/TCP and β-TCP

materials with different formation mode; however, there wasn’t any bone

tissues in HA. Conclusion: TSH/TCP is a biocomposite which can be

shaped into any shape according to different needs, and it has strong

osteogenic ability in mice.


- 55 -



Time: 13:30-16:00


Topic: “Microorganism and Ecosystem”

Session Chair: Assoc. Prof. Siew Woh Choo

T0003

Presentation 1

(13:30~13:45)

Deforestation across the World: Causes and Alternatives for Mitigating

Alexandre Marco da Silva and John Rodgers

São Paulo State University, Brazil

Abstract - We underline the main causes of deforestation and present a

framework for mitigating the continual loss of forest habitat around the

world. The causes of deforestation are complex and they operate at

multiple spatial and temporal scales, yet they are related to societal and

political factors. Societal factors consist of human migration within rural

settings and poverty. Political factors include ineffective land management

and negligence of monitoring and protecting forest commodities.

Combined, these factors present arduous challenges to protecting forest

habitats. In turn, there are at least three strategies to reduce further forest

loss. The first strategy involves the cessation of timber cutting and

extraction of other forest products. But just as important is the second

strategy of creating and maintaining more Environmental Protected Areas.

The third strategy is perhaps the greatest challenge, and it involves the

adoption of the “green nudge principle”, whereby individuals are educated

to modify individual behaviors related to patterns of consumption and to

the conservation of remaining forests. This is especially true for the

increasingly endangered primary forests. An adoption of these mitigation

strategies will help reduce deforestation and will help improve global

environments.

T0006

Presentation 2

(13:45~14:00)

Developing Ecosystem Accounts as Basic Information System for

Monitoring the Changes of Peatland Ecosystem in Indonesia

Resti Salmayenti


- 56 -

Wageningen University and Research, The Netherlands

Abstract—Indonesian peatlands only cover 8% of Indonesian surface but

provide enormous benefits for human throughout agricultural sector.

However, activities in peatlands lead to various environmental impacts like

land degradation. Plenty studies about peatlands have been conducted,

however, available information is not developed in an integrating system

for giving insight the changes of environmental aspects and economic

activities. This research applies SEEA-EEA framework to monitor the

ecosystem changes concerning their physical and monetary values. This

study starts indicating the changes of eight-peatland cover (1990-2014) in

extent account, 52% of forest area in 1990 have been converted to other

land uses. Next, ecosystem condition account explains the changes of

environmental states using four indicators (precipitation, burned area,

degraded land and forest biomass). Third, ES account indicates seven-main

ecosystem services (oil palm, biomass for pulp, paddy, timber,

CO2-sequestration, biodiversity habitat and nature watching). These

services are worth around €5.5-billion (2000) and increased to €7-billion

(2014). Oil palm production has been expanded significantly, contributed

to 49% of total value in 2014. Carbon account shows that CO2-emission

highly increased from 210Mt-CO2/year (€5.8-billion) (2000) to

385Mt-CO2/year (€10.8-billion) (2014). Lastly, ecosystem asset presents

the estimated NPVs of paddy field, oil palm and acacia plantation in

20-year period.

T0014

Presentation 3

(14:00~14:15)

Investigation on Thermochemical Behavior of Oil Palm Residues and their

Bio-Oil from Pyrolysis Process

Yanisa Chantanumat

Prince of Songkla University, Thailand

Abstract—The palm oil industry generates an abundance of oil palm

residues namely empty fruit bunch, mesocarp fiber and kernel shell. These

residues are one of the biomass resources that can be utilized for the

production of bio-oil or chemicals via the pyrolysis process. Thermal

behavior of palm empty fruit bunches (EFB), palm mesocarp fiber (PF)

and palm kernel shell (PKS) was investigated using thermogravimetric

analysis (TGA) conducted under N2 atmosphere at a heating rate of

10°C/min from 50 to 600°C. Palm empty fruit bunches, mesocarp fiber

and kernel shell were pyrolyzed in a fixed bed reactor at 500 °C for 60 min

under the flow rate 400 mL/min of N2 as carrier gas. The chemical

composition of bio-oil was analyzed by a gas chromatography/mass

spectrometry (GC/MS). The thermal profiles of the three residues appeared

to correlate with the cellulose, hemicellulose and lignin content in the

samples. A great range of chemicals such as hydrocarbons, ketones,

aldehydes, acids, phenols and nitrogen compounds were indicated in PKS,


- 57 -

EFB and PF bio-oil. Pyrolysis oil obtained from PKS, EFB and PF has the

potential to be utilized as chemical feedstocks or possibly upgraded for

fuels.

T0021

Presentation 4

(14:15~14:30)

Landscape capacity for supplying ecosystem services in Taiwan Alishan

National Scenic Area

Fu-Chiang Yang and Yi-Tui Chen

National Taipei University of Nursing and Health Sciences, Taiwan

Abstract—For some service facilities, the unused service capacity cannot

be stocked overnight and will become a burden to the service provider. The

main purpose of this article is to analyze the effects of seasonality on

capacity choice decisions and examine the firm’s pricing strategies. We

present a mathematical model and find that higher operating costs lead to

lower service fees in booming seasons and higher service fee in normal

seasons, but the capacity should be decreased; the higher proportion of

booming seasons in a year results in higher service fee in booming seasons

and lower service fee in normal seasons, and the capacity should be

increased. The price markup in booming seasons is higher if the

proportion of booming seasons increase, but lower if operating costs

increase.

T0011

Presentation 5

(14:30~14:45)

Optimization of indole-3-acetic acid production by diazotrophic B.subtilis

(KP455653), isolated from rhizosphere of Eragrostis cynosuroides

Sonali Kumari, Chander Prabha, Abha Singh, Sushma Kumari, and

Shilpi Kiran

Patna University, India

Abstract—Diazotrophic rhizobacteria, trigger and enhance plant growth as

well as yield through various mechanisms, so their use can reduce the

application frequency of chemical fertilizers. Indole-3-acetic acid (IAA), a

most common natural auxin influences several physiological processes of

the plant’s health. The present study is aimed to optimize the conditions for

IAA production, along with assay for plant growth promoting traits of

Bacillus subtilis DR2 (KP455653), which is a diazotrophic Gram positive,

rod bacterium, isolated from rhizosphere of road side weed, Eragrostis

cynosuroides from Danapur, Patna, Bihar, India. The screening for IAA

production was done in JNFbˉ broth with tryptophan (1 g.l-1

) and without

tryptophan at pH 5.8, 30±2 °C temperature and 48 h incubation. 137.81

µg.ml-1

and 100.26 µg.ml-1

IAA was produced in Trp+ and Trp

- media,

respectively. Under various optimized conditions, maximum IAA was

produced at 96 h incubation (137.81 µg.ml-1

), 35 °C temperature (141.92

µg.ml-1

), pH 7 (158.79 µg.ml-1

), mannitol as carbon (160.85 µg.ml-1

) and

ammonium sulfate as nitrogen (162.93 µg.ml-1

) sources with tryptophan at


- 58 -

final concentration of 1.2 µg.ml-1

(168.09 µg.ml-1

), which enhanced the

production by 1.2 fold. The findings suggest that B. subtilis DR2 is a

potent organism to be used as biofertilizer.

T0013

Presentation 6

(14:45~15:00)

Isolation and Characterization of thermostable amylase producing bacteria

from hot springs of Bihar, India

Shilpi Kiran, Abha Singh, Cahander Prabha, Sushma Kumari, and Sonali

Kumari

Patna University, India

Abstract—Amylases are starch degrading enzyme with wide industrial

applications. Microbial production of amylase is more simplified and

economical than other sources. In this study, amylase producing bacteria

were isolated from hot springs of Munger (Bihar, India). Water samples

were collected from three different kunds, viz., Sita kund, Rishi kund and

Bhimbandh kund with temperature 40-50°C and pH 5.2-6.3. Amongst

amylase positive isolates, RK6 was selected for amylase characterization,

exhibiting enzyme activity at highest optimum temperature of 80°C. On the

basis of 16S rRNA gene sequence, it was identified as B. subtilis RK6

(KX247637). Amylase of RK6 was characterized with optimum pH of 8.0

and was thermoactive, retaining its activity upto 90°C and stability at

60-90°C after preheating for 30 min. Vmax and Km for enzyme activity were

60.56 U/ml and 1.86 mg/ml respectively, showing an appreciable affinity

for substrate. The enzyme was activated in the presence of Ca2+

, Mg2+

, and

Fe2+

, while that of Zn2+

and Cu2+

resulted in its inhibition. The present

finding indicates potential of thermo-alkaliphilic amylase of B. subtilis

RK6 for various biotechnological applications.

T2005

Presentation 7

(15:00~15:15)

Evaluating Hydrophobic Bacteria as a Potential Adsorbent for Oestrogen

Removal in Wastewater Treatment

Nur Nasimi Abdul Latif, Dr. Paul Sallis and Dr. Russell Davenport

Newcastle University, United Kingdom

Abstract—Oestrogens are endocrine disrupting compounds known to

produce an adverse effect on the aquatic population by altering their

reproductive functions, even at very low concentration i.e. part per trillion.

This study makes use of the hydrophobicity and obtains highly

hydrophobic bacterial strains that can adsorb oestrogens to a level that has

no health and ecological implications. Strains of Rhodococcus erythropolis

were adapted using hydrocarbons and new bacterial species were isolated

from a diesel-contaminated soil through soil-enrichment. N-hexadecane

and hexane (hydrocarbons) were used as substrates.

This study shows that the Rh. Erythropolis DSM311 adapted in

n-hexadecane had 6.7% Cell Surface Hydrophobicity (CSH) increment


- 59 -

compare to its parent strain and the soil-isolated SD2-1 strain showed

91.33% CSH. Moreover, the stationary phase bacteria (collected at day 10

incubation) were found to have a higher CSH and produced higher

oestrogen removal compared to the exponential phase cell (collected at day

5). An optimization study revealed that 50mg of SD2-1 strain collected at

the stationary phase was found to be the optimum condition for 95.3%

oestrogen removal. This performance was almost comparable to the

activated carbon adsorbence. In conclusion, higher CSH bacterial strains

adapted with hydrocarbon are more efficient in removing oestrogen

compared to the low CSH strains.

T1002

Presentation 8

(15:15~15:30)

Enhanced Phytoextraction of Cadmium by Dianthus Chinensis with

Biodegradable Chelators for Soil Remediation

Erh-Hsuan Tsai and Zeng-Yei Hseu


Abstract—Phytoextraction is a technique by harvesting the plants which

can remove contaminants from soils. Different chelators have been used to

increase the bioavailability of heavy metals and enhance the efficiency of

phytoremediation. Rainbow pink (Dianthus chinensis Linn.) has been

proved to accumulate a high amount of Cd from soil, and thus this study

explored the Cd accumulation in Dianthus chinensis treated with different

biodegradable chelators in Cd contaminated soils (10 and 20 mg Cd/kg) for

50 days. The tested chelators were N,N'-disuccinic acid (EDDS),

3-Hydroxy-2,2'-Iminodisuccinate (HIDS), citric acid (CA), and succinic

acid (SA). The experimental results indicated that the soluble Cd

concentration was the highest in the EDDS treatment and up to 367 μg/L,

followed by HIDS, CA, and SA in the 10 mg Cd/kg soil. However, all

chelator treatments were significantly higher than control. In the 20 mg

Cd/kg soil, the soluble Cd concentration in all chelator treatments exceeded

1000 μg/L. There was no significant (P < 0.05) difference on plant weight,

chlorophyll and proline contents, and the reactivity of triphenyltetrazolium

chloride (TTC) in roots in all chelator treatments, but different efficiency

of Cd uptake existed among them. The maximum Cd concentration in the

shoot was 52.8 mg/kg in the EDDS treatment, followed by CA, SA and

HIDS treatment, while the control was only 31.7 mg/kg. The results shows

that the addition of biodegradable chelators can be used for the enhanced

phytoextraction of Cd and EDDS is the best chelator in this study.

T0004

Presentation 9

(15:30~15:45)

Synthesis and Water-Repelling Properties of New Acrylates with Short

Fluorinated Alkyl Chains

Eun-kyung Kang, Eun-Ho Sohn, and Byung Min Lee

University of Science and Technology, Korea


- 60 -

Abstract—Fluorine compounds which are stable to high temperature,

strong acid and base have been widely used in many fields because of their

excellent surface properties. Fluorinated compounds with long

fluoro-carbon chains, especially perfluorooctanoic acid (PFOA) and its

derivatives, are harmful to human health and the environment. The use of

long perfluoroalkyl compounds such as perfluorooctanoic acid (PFOA)

was restricted owing to their non-biodegradable and toxic effects in the

environment. In order to solve these problems, we were investigated the

new repellents having short fluorinated alkyl chains. The structures of

designed products were identified by NMR and FT-IR. Water repellency of

synthetic water-repellent agents was confirmed by measuring the water

contact angle.

T0023

Presentation

10

(15:45~16:00)

Adsorption of Fe Ions from Aqueous Solution Using α-keratin-coated

Alginate Biosorbent

Ganjar Fadillah, Elsa Ninda Karlinda Putri, Syahna Febrianastuti, Easy

Vicky Maylinda, and Candra Purnawan

Institute of Technology Bandung, Indonesia

Abstract—The biosorbent α-keratin-alginate (KA) was prepared by using

the encapsulation technique in CaCl2 2 % (w/v) solution. The biosorbent

was characterized by Fourier Transform Infrared (FTIR) and Scanning

Electron Microscope (SEM). The extent of adsorption was found to be a

function of the composition of α-keratin and alginate, the pH of solution

and contact time. The optimum adsorption of Fe ions in aqueous solution

was found at the composition of α-keratin and alginate of 1:2 (w/w), the

pH at 7.0 and contact time at 60 minutes. The adsorption of Fe ions on KA

biosorbent was comparatively higher than α-keratin and alginate only. The

adsorption capacity of KA biosorbent has the maximum adsorption

capacity of 658.4 mg/g while biosorbent α-keratin and alginate are 464.7

mg/g and 528.1 mg/g, respectively. Adsorption of Fe ions in aqueous

solution followed the Freundlich adsorption isotherm, and the dynamic

adsorption model could be described through a pseudo-second order

kinetics.

Coffee Break

16:00~16:20 Lounge in Front of C5


- 61 -



Time: 16:20~18:50


Topic: “Gene Expression and Analysis”

Session Chair: Prof. Egils Stalidzans

B0081

Presentation 1

(16:20~16:35)

Comparative Co-expression Analysis between Soybean and Arabidopsis

Zhixing Zhu, Jing Peng, Yuhua Fu, Yangjing Gan and Daipeng Luo

Wuhan University of Technology, China

Abstract—Arabidopsis thaliana, an important model species, is widely

used in biology research, and employing its knowledge to study other

related species is one of the major applications. Soybean is a major legume

crop and it is of great economic and nutritional value. Therefore, many

co-expression databases including these two species are constructed. But,

there is little research focused on the differences of their orthologous gene

co-expression relationship. In this paper, we calculated the general

co-expression coefficient of any two genes in orthologous genes, compared

the differences of this coefficient between Arabidopsis and Soybean, and

analyzed the similarity of these two species. Although many studies on

Soybean are based on the orthologous relationship with Arabidopsis, our

results show that there only about 38.85% gene pairs are similar between

the two species. Our study indicates that researchers should have an eye on

these existed differences when conducting research on Arabidopsis and

Soybean.

B0086

Presentation 2

(16:35~16:50)

Genome Scale Metabolic Model of Arabidopsis Thalina for Isoprenoid

Production

Agris Pentjušs and Egils Stalidzans

Latvia University of Agriculture, Latvia

Abstract—Capacity of plants of converting light to different valuable

products makes them highly relevant to “green factories”. To reduce

burden of arable land usage for valuable product production there is need


- 62 -

to increase significant plant productivity.

In this study to we use Arabidopsis thaliana genome scale model in a

synthetic biology based approach to increase production of z-abienol and

isoprene depending on light intensity and diurnal cycle. Genome scale

model is primarily derived from annotation aracyc 13.0 in the Aracyc

database. We are using linear programming to demonstrate model

capability to produce biomass components (amino acids, nucleotides,

lipids, starch, cellulose). Model structure uses cytosol, mitochondria,

plastid, vacuole and peroxisome compartments. Compartmented reactions

are mostly used for interaction analysis of light and diurnal cycle involved

reactions.

Kinetic model of MEP as plant isoprenyl precursor pathway located in

plastids will be used in combination with genome scale model to assess the

feasibility of kinetic model steady states suggested by optimizations.

B0088

Presentation 3

(16:50~17:05)

Molecular Cloning and Comparative Analysis of Variable Regions of

Monoclonal Antibody against Enrofloxacin Clone 48

Jirawat Mala, Songchan Puthong, Kittinan Komolpis and Sarintip

Sooksai

Suratthani Rajabhat University, Thailand

Abstract—Enrofloxacin is fluoroquinolone antibiotic which prohibited

approved for treatment in animals. However, their residues in animal

products can pose adverse side effects to consumer. Therefore, the

maximum residue limit of these drugs has been enforced in many

countries. In this study, the cDNA encoding VH and VL genes was

amplified from monoclonal antibody which was specific to enrofloxacin

clone 48, cloned and sequenced. The obtained sequences were compared in

the NCBI databases by using blastp program. The results found that VH

nucleotide was composed of about 399 bps and theirs deduced amino acids

showed 80-85% degree identities to the Ig superfamily group. A totally 356

bps of VL nucleotide was found and showed the degree of identities of

97-100% with an immunoglobulin kappa light chain. Moreover, the CDR I,

CDR II and CDR III of the VH and VL sequences were specified. The

results indicated that the highest degree of VH sequence homology (highest

relationship) found in accession no. B26471 with 80% homology.

However, 100% of VL sequences were found similarly to accession no.

AHJ10945.1 than the others. The obtained results provided the useful and

important information for the further recombinant antibody construction

and production against enrofloxacin antibiotic.

B0114

Presentation 4

Feasibility of Using Hypersaline Lake Sediment as Inoculum for Biogas

Production from Anaerobic Digestion of Saline Wastewater

Manal Ali, Ahmed Elreedy and Ahmed Tawfik


- 63 -

(17:05~17:20)

Egypt-Japan university of science and technology (E-JUST), Egypt

Abstract—Anaerobic digestion processes have been widely used for biogas

production from various substrates, however, without much attention to the

saline effluents containing organic compounds. This study aims to

investigate the use of hypersaline lake sediment as inoculum, after a

hierarchical acclimation process, at different inoculum-to-substrate ratios

(ISRs) and salt (NaCl) concentrations. Batch-mode experiments were

conducted to achieve this goal using glucose as a substrate. ISRs ranging

from 11.5 to 28.74 gVSS/gCOD and NaCl concentrations of 20-30 g/l were

tested. Maximum cumulative biogas production (CBP) of 55 ml was

achieved at ISR of 11.49 gVSS/gCOD and NaCl concentration of 30 g/l,

resulting a biogas production rate (BPR) and specific biogas production

(SBP) of 1.08 ml/h and 220 ml/l, respectively. Moreover, at same

conditions, the highest chemical oxygen demand (COD) removal

efficiency of 84.12 % was resulted. The highest acidification corresponded

by maximum production of volatile fatty acids (VFAs) of 1652.2 mg/L was

found at ISR of 11.5 gVSS/gCOD. The acetic acid was predominant with a

maximum concentration of 500 mg/L. A modified Gompertz equation

successfully described the time-course cumulative biogas production (R2 =

0.989). Eventually, at optimum conditions, the microbial enriched

sediment collected from hypersaline lakes proved its easy and effective use

as inoculum for biogas production from saline wastewater.

B0076

Presentation 5

(17:20~17:35)

Novel Strategy to Quantify Circular RNA Using Model-Based Framework

Musheng Li, Tong Zhou and Wanjun Gu


Abstract—Circular RNA is a class of widely expressed non-coding RNA

whose both ends covalently bond together to form a circular shape. Some

of them are reported to work as miRNA sponge such as CDR1as. but the

exact functionality of most circular RNA is still unknown. To gain more

understanding of their functionality. circular RNA transcript abundance

in RNA-seq datasets should be estimated. but existing state-of-art

transcript expression quantification tools are not suitable for circular RNA.

Here, we present a strategy that transform circular transcripts into

pseudo-linear transcript and estimates the expression values of both

circular and linear transcripts using existing model-based algorithm named

Sailfish. We test this strategy in both simulated and real rRNA depleted

RNA-seq data. Result shows this new strategy can estimate both linear and

circular transcript accurately. It gives superior performance on compare to

traditional count-based method. Our result also shows that by taking

circular RNA into consideration will increase accuracy of linear transcript

expression when estimating transcript abundance in rRNA depleted

RNA-seq dataset.


- 64 -

B0019

Presentation 6

(17:35~17:50)

Preliminary Genetic Assessment OF Elephas maximus by Using Fecal

Samples from Selected Hotspots in Taman Negara National Parks,

Peninsular Malaysia

Kayal Vizi Karuppannan, Badrul Munir Md Zain, Salmah Yaakop and

Jesús E Maldonado

Department of Wildlife and National Parks (Dwnp) Peninsular Malaysia,

Malaysia

Abstract—One of the main goals in National Elephant Conservation Action

Plan (NECAP) is genetic assessment of Asian elephant in Taman Negara

National Parks, Peninsular Malaysia. As a first step, we examined a total of

38 Elephas maximus fecal samples using microsatellite, molecular sexing

and control region markers to gather all available genetic information.

Twenty eight fecal samples were collected from selected locations in

Taman Negara National Parks and remaining positive control samples were

from known male and female elephants of National Elephant Conservation

Centre (NECC). We use 15 pairs of fluorescently labeled polymorphic

microsatellite and 3 pairs of molecular sexing markers together with

mtDNA marker in this study. Laboratory process were started from DNA

extraction, PCR and completed by fragment analysis. Several analysis

programmes were used in interpreting data and standard genetic

assessment including sex ratio were calculated. The final outcomes of

this experiment will provide information on genotypes and sex ratio of

wild E.maximus in the study area and can be extended for other collected

samples to get a complete database on elephant population structure in

Taman Negara National Parks.

B0115

Presentation 7

(17:50~18:05)

Lymphoma-Associated Gene Expression Changes in an Inducible Model of

Myc-Driven B-Cell Lymphoma

Amir Mahani, Gustav Advirson, Alf Grandien and Anthony P. H. Wright

Karolinska Institute , Huddinge, Sweden

Abstract—The c-Myc transcription factor, identified as a proto-oncogene,

is involved in human malignancies. In Burkitt’s-Lymphoma (BL),

translocation of c-Myc (MycWT) plays a major role in lymphoma

development. In addition, mutations causing substitutions in c-Myc are

associated with BL. Interestingly, the MycT58A and MycT58I mutations

were reported to increase cellular transformation and stability of the Myc

protein. To understand the role of MycWT and associated-BL mutations in

lymphoma, we made three different B-cell lines that express MycWT,

MycT58A, or MycT58I in a doxycycline dose dependent manner. At high

levels of Myc the cell lines display characteristics associated with

lymphoma cells. In this study, we overexpressed Myc at seven different

levels in each cell line and identified cell cycle changes by flow-cytometry


- 65 -

using propidium iodide staining. Changes in cell size and number where

also measured. mRNA was sent for NGS sequencing. Myc dependent

changes in RNAseq data were analysed using generalized linear models to

identify and compare different regulatory patterns (maSigPro package)

associated with the wild type and mutant Myc proteins. GO terms could be

mapped to gene clusters with different responses to Myc and can explain

important changes that occur in lymphomas that are driven by wild type or

mutant Myc.

B0036

Presentation 8

(18:05~18:20)

Use of Low Temperature Cold Atmospheric Plasma in The Treatment of

Melanoma Cells

Pei-Ru Chen, Ming-Chen Wang and Yun-Ju Chuang

Ming Chuan University, Taiwan

Abstract—Recently, cold atmospheric-pressure (CAP) plasma have

become a novel and popular tools in biomedical applications. It has been

proved that CAP plasma treatment will induce cancer cells death. With

different CAP plasma treatment parameters, cells will have different fate

including proliferation, apoptosis and cell membrane damage. It has been

reported that a low energy plasma will cause cell proliferation while a

longer exposures lead to cell death. So, the aim for this study is to induce

melanoma cells apoptosis in a short time by CAP plasma-jet. We first

evaluate the optimal working condition of CAP plasma-jet including

treatment time, voltage, flow-rate and plasma-gas composition. Then we

use CAP plasma-jet treat on melanoma cells with different medium

volume, cell number and treating times to find a suitable condition for the

cell culture. Results demonstrate that with medium 0.6 ml and seeding 104

cells have highest cell viability while treat 180 s and 360 s are significantly

decreased. In the migration assay, cells migration ability is both

decreased when treat with plasma for 180 s and 360 s. We also do the

Hoechst 33258 stain, after treating with CAP plasma melanoma cells are

detach from the bottom and have DNA condensation. Finally, we

examine ROS expression present/ absent ROS inhibitor sodium pyruvate

(SP). Data shows that after CAP plasma treatment ROS expression is

induced while cell viability is reduced. But cell viability is recovered by

pretreat with ROS inhibitor SP. In conclusion, both cell viability and cell

migration ability are inhibited by CAP plasma-jet via ROS mediation in

melanoma cells.

B0099

Presentation 9

(18:20~18:35)

A Study of Immune Function Improvement Induced by Ganoderma

Lucidum Alkaloid in Mice

Lijia Cheng, Zheng Shi and Tian Yu



- 66 -

Abstract—Objective: To study the effect of extractive of Ganoderma

lucidum (G. lucidum) alkaloid on immune function in mice. Methods: 48

female BALB/c mice were randomly divided into 4 groups (n=12), 10

mg/kgd (group A), 50 mg/kgd (group B), 100 mg/kgd (group C) and the

control group (group D). All animals were administered by gavage for 14

days. At last, macrophage phagocytosis ability, T lymphocyte

transformation capacity and interferon-gamma (IFN-γ) levels were

measured. Results: The G. lucidum alkaloids could significantly promote

the spleen/body weight ratio, the proliferation of T lymphocytes, the

macrophage activity and the levels of IFN-γ in BALB/c mice by oral

treatment. Conclusion: G. lucidum alkaloid could improve the immune

function of mice, suggesting that G. lucidum alkaloids is a promising

candidate for promoting immune response and a potentially valuable

substance for pharmacological use.

B0014

Presentation 10

(18:35~18:50)

Modulation of Cellular Properties of Fibroblasts Cultured in High-Glucose

Environments with Low-Intensity Pulsed Ultrasound

Da-Ming Huang and Shyh-Hau Wang


Abstract—Depending on such operational modes as the frequency,

exposure duration, and duty cycle, previous studies have shown that the

insonification of low-intensity pulsed ultrasound (LIPUS) may be

beneficial to the enhancement of wound healing in the skin tissue and to

the cellular properties. In the present study, effect of LIPUS was

investigated on the fibroblasts cultured in high-glucose (HG) environments

for mimicking to that of diabetic patients. Experiments were arranged to

allow LIPUS insonification into the murine fibroblasts, which were

cultured in various medium with the glucose concentrations ranging from

5.5 to 33 mM. The LIPUS consists of 1 MHz frequency, 50 mW/cm2

intensity, 20% duty cycle within 1 ms pulse repetition period, and 10

minutes exposure duration. The cellular properties, including viability and

stiffness of cell membrane, were respectively measured by XTT assay and

nano-indenter. Results show that the growth and stiffness from the

fibroblasts cultured in HG medium tended to decrease. Remarkably, both

the growth and stiffness of cell membrane of the fibroblasts cultured in HG

environments are improved after the LIPUS insonification. It suggests the

modulation of cellular properties and the improvement of stiffness of cell

membrane are owing to mainly the micro-vibration of LIPUS

insonification.


- 67 -

Session 9




Time: 16:20~18:50


Topic: “Drug Development and Dynamics Analysis”

Session Chair: Assoc. Prof. Jayantha Gunaratne

B0058

Presentation 1

(16:20~16:35)

Degradation Kinetics of Cyanide and Uric acid in Bamboo Shoot during

Boiling Process

Sawanit Aichayawanich, Chanthima Phungamngoen, Jittimon Wongsa

and Thanya Parametthanuwat

King Mongkut’s University of Technology North Bangkok, Thailand

Abstract—The effects of boiling temperatures (60, 70, 80, 90, and 100oC)

and shape (whole shoots, slices, and rectangular rods) on the degradation

of cyanide and uric acid in bamboo shoots during the boiling process were

investigated. Boiling temperature was found to enhance cyanide and uric

acid degradation. While, the shape of the bamboo shoot effect on the

degradation of cyanide and uric acid on the whole shoot is lower than that

of sliced and rectangular shaped shoots, respectively. Kinetic modeling of

cyanide and uric acid degradation indicated that the degradation of cyanide

and uric acid could be defined using first-order and zero-order models,

respectively. Moreover, the Arrhenius equation can explain the effect of

temperatures on kinetic rate constant of cyanide and uric acid degradation.

The consumers accept the sensory properties of boiled bamboo shoots after

boiling for 18 min in all boiling temperature and bamboo shoot shape.

B0067

Presentation 2

(16:35~16:50)

Preparation and Characterization of Ca-alginate-chitosan Capsules for

Controlled Release of Imidacloprid as Larvaecide Delivery System

Lim Gim Pao and Ahmad Muhammad Syarhabil

School of Bioprocess Engineering, Universiti Malaysia Perlis, Malaysia

Abstract—Ca-alginate-chitosan based capsules were prepared and then

loaded with imidacloprid larvaecide for its controlled release against Aedes

aegypti larvae. The Ca-alginate-chitosan capsules containing imidacloprid


- 68 -

were characterized using FTIR and SEM. Imidacloprid encapsulation was

proven through HPLC analysis. Imidacloprid loading and encapsulation

efficiency were 5.9 % and 34 %. The in vitro release test showed that the

usage of Ca-alginate-chitosan capsules positively modified the release

profiles of the imidocloprid in water. The imidacloprid release kinetics fit

the Korsmeyer–Peppas model and follows the anomalous transport profile.

The bioassay results showed that Ca-alginate-chitosan capsule loaded with

imidacloprid is effective against Aedes aegypti larvae at lower doses than

that required by the technical grade imidacloprid for the control of Aedes

aegypti larvae.

B0075

Presentation 3

(16:50~17:05)

Mining Patterns of Drug-Disease Association from Biomedical Texts

Wen-Juan Hou, Bo-Syun Lee and Hung-Chi Chen

National Taiwan Normal University, Taiwan

Abstract—Drug repurposing aims to identify new indications for approved

drugs, and it can promisingly reduce time and drug development costs. The

goal of the paper, drug-disease relation extraction automatically from

biomedical texts, is fundamental to the study of drug repurposing since lots

of clinical case studies published in an unstructured textual form. To

analyze the number of verbs and nouns pertinent to diseases and

medications in the training data, two models with different drug-disease

orders are established, and some rules are proposed at this phase. The first

model is for the sentences with the order that the disease name precedes the

drug name. The second model is for the reverse order to the first model.

These verbs and nouns are then classified into categories of “pure

association,” “pure no association” and “neutrals.” Among them, some

neutrals are further verified by the Chi-square test method. As a result, the

associations between diseases and medications are identified, which are

called patterns later. Finally, the patterns are used in the test data to extract

the disease and drug pairs. The best experimental results show the

precision value of 100%, recall value of 89.0%, and F-score value of

94.2%.

B0083

Presentation 4

(17:05~17:20)

Single Administration of Resveratrol-Loaded Nanoparticles via Carotid

Artery Improves Neuronal Damage and Promotes Neuronal Regeneration

in Transient Middle Cerebral Artery Occlusion Rats

Huae Xu, Jie Zhong, Xiaolin Li and Xiaowei Lu

The First Affiliated Hospital with Nanjing Medical University, China

Abstract—Endovascular thrombectomy (EVT) retract clots and open

occluded arteries rapidly usually leads to ischemia/reperfusion (I/R) injury

which discounts the beneficial effect. Resveratrol (RES) was reported to

attenuate oxidative stress, inflammation and apoptosis related to I/R injury.


- 69 -

However, its application was restricted by poor water solubility, short

half-life in vivo and low concentration in brain. We encapsulated RES in

biodegradable Poly(N-vinylpyrrolidone)-b-poly (ε-caprolactone)

nanoparticles (RES-NPs) and tested their efficacy in a rat Intraluminal

filament transient middle cerebral artery occlusion (tMCAO) model. A

carotid artery route of administration was applied to maximize drug

localization in the brain. Localized brain delivery of RES-NPs reduced

infarct volume, maintained BBB integrity and relieved edema through

neutralized ROS, atteneuated apoptosis. Neither NPs mixed with RES-Sol

nor RES-Sol alone was effective to protect brain. Survived rats treated with

RES-NPs demonstrated neurogenesis and regained vital neurological

functions.

B0087

Presentation 5

(17:20~17:35)

Superior Therapeutic Efficacy of Self-Assemble Paclitaxel Nano-Filaments

through Enhanced Induction of Autophagy in Gastric Cancer

Huae Xu, Wenling Zhang, Na Yu, Xiaolin Li and Weihao Sun

The First Affiliated Hospital with Nanjing Medical University, China

Abstract—The side effect of Paclitaxel (Ptx) has limited its clinical

application because of poor solubility. In the current study, we designed

novel self-assembled paclitaxel nano-filaments with extremely high drug

loading efficiency by conjugating Ptx and succinic acid (SA). The

conjugate Ptx-SA could self-assemble into nano-filaments with excellent

water solubility. Cytotoxicity test showed the superior effect of Ptx-SA

against gastric cancer cell lines SCG7901 and BGC823. Ptx-SA more

efficiently induced the apoptosis and autophagy-induced cell death than the

equivalent dose of free Ptx. More autophagosomes with increasing

autophagic flux was elicited by Ptx-SA in gastric cancer cells than free Ptx.

Ptx-SA induced the expression of LC3, a marker of autophagy, more

effectively than free Ptx. Moreover, Ptx-SA nano-filaments could

significantly enhance the in vivo anti-cancer effect of Ptx. Therefore, the

self-assemble nano-filaments of Ptx-SA is a potential way to strengthen the

therapeutic efficacy of Ptx in curing gastric cancer.

B0096

Presentation 6

(17:35~17:50)

Studies and Biological Significances of Medical Plant Microecology

Guo Xiaoheng and Yan Zhuyun


Abstract—The relationship between plants and microbes began in

mycorrhizal, which involves in plant physiological and biochemical

metabolism and gene regulation. Due to the improvement of plant

nutrition, stress resistance, the safety of ecological environment,

maintaining and enhancing the sustainable productivity of ecosystems, it

has been widely concerned from abroad; Meanwhile, it was found that


- 70 -

endophytic bacteria involved in plant physiological and biochemical

metabolism and co-metabolism, with the growth promotion and biological

control. Rhizosphere beneficial microbes and roots form a mutualistic

relationship, which can improve plant nutrition, growth, cological and

environmental safety. Beneficial phyllospheric microorganism participates

in plant carbon and nitrogen metabolism, enhancing disease resistance,

insects, etc. This paper summarizes some common and specific problems

of endophytes, mycorrhizal fungi and rhizosphere microorganisms, and

points out the development trend of research. This paper explores the scope

of microbial research of medicinal plants, and indicates that the life history

of plants accompanies with microbial nutrient cycling. It demonstrates that

the microecosystem of medicinal plants consists of microorganisms

(rhizospheric microorganisms, endophytes and phyllosphere microbes) and

their host-related microenvironment (tissue, cell, metabolite, secreta), that

is, a single medicinal plant is a Microecology. The article points out that

"herbs are the product of medicinal plant microecosystem, the quality of

medicine depends on the function of plant microecosystem", and points out

the focus of plant microecological research. The study of medicinal plant

microecology has unveiled plant microecological relationships and a new

way of microbial resources, which will have a wider impact in a number of

areas. The real significance of microbial research in medicinal plants lies in

the use of microbes and host plants to interact with each other to produce

safe and effective medicinal herbs to ensure the quality of traditional

Chinese medicine and realize the sustainable production and utilization of

Chinese herbal medicines.

B0027

Presentation 7

(17:50~18:05)

Study of Recyclability of EMIM-Ac in Rice Straw Pretreatment

Ratsarin Akkharasinphonrat, Kraipat Cheenkachorn, Surapun

Tepaamorndech, Atthasit Tawai and Malinee Sriariyanun

King Mongkut’s University of Technology, North Bangkok, Thailand

Abstract—Pretreatment is a vital process for the conversion of

lignocellulose biomass into energy. The pretreatment process is known to

enhance solubilization and dissociation of the complex lignocellulose

structure. This study focused on efficacy of ionic liquid pretreatment, using

1-ethyl-3-methylimidazolium acetate (EMIM-Ac), of rice straw for sugar

production in the comparison with acid and alkaline pretreatment. The

results showed that EMIM-Ac pretreatment resulted in dramatic changes in

chemical structures and microscopic morphology of rice straw. These

observations were associated with the high levels of sugar production.

Evaluation of EMIM-Ac recyclability demonstrated that absolute methanol

was an anti-solvent to yield the higher sugar levels than water and acetone.

In summary, these findings show that EMIM-Ac ionic liquid is the efficient

method for rice straw pretreatment due to high sugar yields and


- 71 -

recyclability.

B0055

Presentation 8

(18:05~18:20)

A Potential Therapeutic Rutaecarpine Derivatives for Inflammation-

Induced lung Dysfunction

Chi-Ming Lee, Chiao-Han Yen and Chun-Mao Lin

Taipei Medical University, Taipei, Taiwan

Abstract—Lung fibrosis is a pathological state of excessive disposition of

extracellular matrix in an organ or tissue undergoing series process of

coagulation, inflammation, myofibroblast activation and continuous

reparative or reactive process. Rutaecarpine is the main effective

component of Evodiarutaecarpa which is the wide-used traditional Chinese

medicine. It presents vasodilation、anticoagulation and anti-inflammation

activity. However, the further application is limited by its cytotoxicity.

Thus, we had designed and synthesized a structural modifications of

rutaecarpine (RUT), 10-Fluoro-2-methoxy-rutaecarpine (F-RUT), showing

well anti-inflammation, anti-invasion and anti-migration activity with very

low cytotoxicity in cells. We also observed a raised level of Transient

receptor potential vanilloid type1 (TRPV1) and eNOS in F-RUT-treated

cell. These results suggest that RUT derivatives’ potential ability in

preventing vasorelaxation/hypertension. Particularly, the F-RUT

significantly reduced OVA/alum-induced lung inflammation and the

thickness of the alveolar wall in B6 mice, and also reduced the ROS in

LPS-stimulated zebrafish. The results reveal that F-RUT might be able to

prevent lung fibrosis. Based on the limited options for fibrosis treatments,

this novel compound provides a new insight to be a pharmaceutical

strategy of fibrosis mediated by inflammatory reaction, such as asthma.

B0071

Presentation 9

(18:20~18:35)

Development of Vi Polysaccharide Purification Method for Typhoid

Conjugate Vaccine Material: Effect of Ethanol Precipitation Variation to

Impurities and Vi Polysaccharide

Dea Marsendah and Wardono Niloperbowo

PT Biofarma, Indonesia

Abstract—One way to overcome typhoid fever is by immunization using

Vi polysaccharide vaccines. Purification method of Vi polysaccharide has

been developed but effectiveness not meet satisfactory since high

impurities still present. For that reason, research on purification method of

Vi polysaccharide was developed with focuses on the effect of ethanol

precipitation variation to impurities and Vi polysaccharide. Different

concentration of ethanol solutions (20%, 30%, 40% and 50%) were used to

remove impurities. Based on the measurement of endotoxin value and

nucleic acid from samples, precipitate washed with ethanol 30% gave

endotoxin value of 60% fraction at 261.7 EU/mL and nucleic acid at 0,243


- 72 -

mg/mL and it is the lowest among others. In this research also conducted 3

times repeated washing treatment with 20% and 30% ethanol solutions.

Precipitate washed 3 times with 20% ethanol gave endotoxin value of 60%

fraction at 139.3 EU/mL, while washing with ethanol 30% gave endotoxin

value at 102.5 EU/mL. Based on results, it can be concluded that addition

of 30% ethanol solution and repeated three times precipitation process

using 20% and 30% solutions can increase the removal of impurities

without reducing the gain of Vi polysaccharide.

B0079

Presentation 10

(18:35~18:50)

Continuous Quantitative Measurement of Lactic-Acid released from an in

vitro Mouse Brain Slice using Electrochemical Biosensor

Muhammad Izzat Firdaus bin Zailuddin, Shoto Kamada, Yoshiki Tojyo,

Ichiro Takashima, Hiroyuki Kudo and Riichi Kajiwara


Abstract—Although researches for lactic acid as an alternative energy

substrate have increased, its role during many neuronal activities states

remains unclear. The purpose of this research is to design the experimental

system for monitoring the lactic acid slightly released from a living brain

slice (350µm thickness). We combined the interface chamber for

electrophysiology with the microfluidic device where lactate sensor was

implemented. Lactate sensor has an electrode modified with the enzyme

Lactate Oxidase that has high selectivity and sensitivity towards lactate. By

using this system, we successfully measured the lactate signal (1.8 nA)

from the brain tissue under the perfusion of aCSF. Moreover, the signal

was dramatically increased to 7.7 nA when we decreased the glucose level

of the aCSF (10mM to 5mM). These results suggest that the brain slice

normally released a certain amount of lactic acid, and neuro-glial

interaction system in the brain might be triggered by the decrease of the

glucose level of the extracellular space. Since our system is capable for

simultaneous recordings of lactate signal and electrical activity of neurons

following electrical and/or chemical stimuli, it would be useful to

investigate the relationship between metabolism and neuronal activities

under various conditions.


- 73 -

Session 10




Time: 16:20~18:50


Topic: “Environmental Quality Assessment and Management”

Session Chair: Prof. Zeng-Yei Hseu

T0010

Presentation 1

(16:20~16:35)

Double Environmental Management: Cationic Dye Removal on Sorbents

Obtained from Poly(Methyl Methacrylate) Waste

Dominik Zdybał, Małgorzata Skorupa, and Andrzej Milewski

Silesian University of Technology, Poland

Abstract—A new synthesis procedure for macroporous hydrogel was

developed. A possibility has been proven to obtain crosslinked, highly

water-swellable polymer matrix directly from non-crosslinked,

hydrophobic poly (methyl methacrylate) by a simultaneous

hydrolysis-crosslinking process in DMSO-diglyme solvent system. Waste

samples of PMMA, provided by automobile industry, have been harnessed

to obtain crosslinked poly (methacrylic acid)-co-poly (methyl

methacrylate) macroporous sorbents. Pure atactic PMMA was also used

to obtain a series of sorbents in order to optimize the synthesis conditions.

Rhodamine B was used as a model cationic dye for kinetics studies,

isotherm evaluation and to determine pH-dependent ability to remove the

contamination. Nano/micro-Fe3O4 fillings were added to amend sorbents

reuse by magnetic sedimentation process. Regeneration of sorbents has

been performed using a green solvent system. In summary, a new strategy

has been proposed to manage plastic waste, remove contamination from

effluents and reuse the dye.

T3001

Presentation 2

(16:35~16:50)

Magnetic Amphiprotic Catalysts and Separators Coping with

Environmental Issues

Byung Kwon Kaang and Won San Choi

Hanbat National University, Korea

Abstract—Amphiprotic catalysts have recently gained increased interest


- 74 -

regarding applications in oil/water separation and pollutant purification.

Many industries emit oil/water mixtures containing pollutants as

wastewater. The amphiprotic catalysts reported thus far have used

separated processes for oil/water separation and pollutant purification,

which is not suitable for industrial applications requiring in-situ and

continuous processes for oil/water separation and pollutant purification.

Amphiprotic catalysts that can freely move between two phases, such as oil

and water, to perform functions in each phase, such as the decomposition

of aqueous and non-aqueous pollutants, have not been reported. Here, we

report a novel approach-based magnetic amphiprotic catalyst (MAC) that

can freely move between oil and water to perform functions in each phase,

enabling the in-situ and continuous processes for oil/water separation and

the decomposition of aqueous and non-aqueous pollutants in both phases.

T3002

Presentation 3

(16:50~17:05)

Surface Design of Separators for Oil/Water Separation with High

Separation Capacity and Mechanical Stability

Nara Han and Won San Choi


Abstract—A convection heat treatment that can replace existing chemical

oxidation methods was developed for the preparation of hierarchically

oxidized Cu meshes with various surface morphologies, representing a

very simple and green route that does not involve toxic chemicals. Three

types of Cu meshes [bumpy-like (BL) and short and long needle-like (NL)

structures] exhibited similar separation efficiencies of 95−99% over 20

separation cycles, as indicated by their similar water contact angles

(WCAs; 147−150°). However, these Cu meshes exhibited different flux

behaviors. Excessively rough and excessively smooth surfaces of the Cu

mesh resulted in increased resistance to flow and to a decrease of the

penetration of oil. A surface with intermediate smoothness, such as the

BL-Cu mesh, was necessary for high flux over a broad range of oil

viscosities. Furthermore, a less rough surface was more suitable for the

separation of highly viscous oil. Computational fluid dynamics (CFD)

simulations were carried out to support our experimental results. The

BL-Cu meshes also showed outstanding mechanical stability because of

their low resistance to the flow of fluids.

T3003

Presentation 4

(17:05~17:20)

Self-floating and Submerging Sponge Adsorbents for Environmental

Remediation

Yoseph Lee and Won San Choi


Abstract—Water pollution by heavy metals and organic dyes has become a

serious environment issue. Diverse technologies have been reported to


- 75 -

eliminate these pollutants. Among these methods, nanoadsorbents are of

particular interest in wastewater treatment because of their high

surface-to-volume ratio, which leads to a higher uptake capacity. However,

most of the reported nanoadsorbents are flow type, which are very

vulnerable to leakage in the process of separation because they are

dispersed or precipitated. It has been recently reported that the unintended

leakage of nanomaterials into the environment can cause a significant

threat to the environment and public health. Here, we report a janus sponge

composite that can be partially floated/submerged on/in the water to

perform efficient adsorption of pollutants without leakage of adsorbents.

The janus sponge composites not only prevent adsorbents leakage but also

enable to remove heavy metal ion and carry out oil-water separation at the

same time.

T3004

Presentation 5

(17:20~17:35)

Accounting for the Effect of Temperature in Clarifying the Response of

Foliar Nitrogen Isotope Ratios to Atmospheric Nitrogen Deposition

Guoan Wang and Chongjuan Chen

China Agricultural University, China

Abstract—Atmospheric nitrogen deposition affects nitrogen isotope

composition (δ15

N) in plants. However, both negative effect and positive

effect have been reported. The effects of climate on plant δ15

N have not

been corrected for in previous studies, this has impeded discovery of a true

effect of atmospheric N deposition on plant δ15

N. To obtain a more reliable

result, it is necessary to correct for the effects of climatic factors. Here, we

measured δ15

N and N contents of plants and soil in Baiwangshan and

Mount Dongling, north China. Atmospheric N deposition in Baiwangshan

was much higher than Mount Dongling. Generally, however, foliar N

contents showed no difference between the two regions and foliar δ15

N was

significantly lower in Baiwangshan than Mount Dongling. The corrected

foliar δ15

N after accounting for a predicted value assumed to vary with

temperature was obviously more negative in Baiwangshan than Mount

Dongling. These results indicated that atmospheric N deposition had a

negative effect on plant δ15

N. Additionally, this study also showed that the

corrected foliar δ15

N of bulk data set increased with altitude above 1300 m

in Mount Dongling, this provided an another evidence for the conclusion

that atmospheric N deposition could cause 15

N-depletion in plants.

T3007

Presentation 6

(17:35~17:50)

A Novel Sulfide Control Strategy Using Conductive Iron Oxide in

Anaerobic Co-Digestion of Macroalgal Ulva Biomass with Cheese Whey

Heejung Jung, Jaai Kim, and Changsoo Lee

Ulsan National Institute of Science and Technology (UNIST), Republic of

Korea


- 76 -

Abstract— Anaerobic digestion of sulfur-rich feedstock entails a concern

with sulfate reduction which produces sulfide, toxic to methanogenesis.

Conventional strategies for sulfide removal via precipitation by using iron

species are expensive and inefficient. This study studied the potential of

new approach using magnetite, conductive iron oxide, for sulfide control in

the co-digestion of sulfur rich Ulva biomass with cheese whey. Compared

to the control reactor without magnetite addition (0.55–0.83% H2S in

biogas, v/v), the magnetite-added reactor showed significant decreases in

the H2S content down to <0.02% (v/v) with increase in magnetite dosage

(0–8 mM Fe). The Raman spectra of the magnetite-added reactor effluent

corresponded the peak profile of elemental sulfur, but not that of iron

sulfide, which means that sulfate from the Ulva degradation was likely

transformed to elemental sulfur in the presence of magnetite. This likely

suggests that the oxidation of sulfide, produced by sulfate reduction, to

elemental sulfur occurred in the reactor possibly by direct interspecies

electron transfer between electroactive bacteria via magnetite. Although

the detailed mechanism is unclear, this suggests an interesting potential for

in-situ sulfide control and sulfur recovery.

T0016

Presentation 7

(17:50~18:05)

Organizational Carbon Footprint of King Mongkut's University of

Technology Thonburi

Pisitpong Intarapong and Amnat Chidthaisong

King Mongkut’s University of Technology Thonburi (KMUTT), Thailand

Abstract— A comprehensive carbon footprint assessment of organization

has been analyzed from direct and indirect greenhouse gases (GHG) in all

activities. This work aims to evaluate the carbon footprint of an academic

institution in engineering department of King Mongkut's University of

Technology Thonburi (KMUTT), Ratchaburi campus as a residential

college in Thailand and to develop alternative opportunities for GHG

reduction. A multicriterial assessment from the following activities such as

transportation, electricity consumption, materials uses, solid and water

waste, construction, land use, and other energy usages was considered. The

results showed that total carbon footprint collected from the college in

2015 was 981.16 tCO2e/yr. The average carbon footprint per capita was

2.73 tCO2e/yr. The most GHG emissions of 533.3 tCO2e/yr were caused by

electricity consumption, accounting for 54.36 % of the overall GHG

emissions. Building, land use, and solid waste generated GHG emission of

17.48, 12.14, and 6.39 % of the overall GHG emissions, respectively. The

appropriate strategies of GHG reduction have been proposed in order to

reduce the average carbon footprint per capita less than 2.50 tCO2e/yr.

Furthermore, carbon neutrality should be investigated in the campus.

T4006 Changing Organic Matter Characteristics of Littoral Sediment by Solar

Cell-Combined Sediment Microbial Fuel Cell


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Presentation 8

(18:05~18:20)

Narong Touch, Hiroki Takata, Satoshi Yamaji, and Tadashi Hibino

Hiroshima University, Japan

Abstract—Metal ion or clay mineral-adsorbed organic matter present in

littoral sediment is known as hardly decomposed organic matter, which is

difficult to use as a natural resource. This study is aimed at changing the

organic matter characteristics of littoral sediment through the application of

solar cell-combined sediment microbial fuel cell (SC-SMFC). The

experimental results showed that the sediment pH decreases and the

concentration of metal ions in the sediment pore water increases after the

application of SC-SMFC. This suggests the dissociation of metal

complexes in the sediment. From the analysis results of organic matter

characteristics, variations in the ignition characteristics of the sediment and

the absorbance at wave number ranges of 3300–3800 and 800–1800 cm-1

were confirmed, indicating changes in organic matter characteristics of the

sediment. It can be concluded that SC-SMFC can separate organic matter

from metal complexes, leading to the transformation of organic matter in

sediment from valueless into a useful product.

T0028

Presentation 9

(18:20~18:35)

Developing Hydrodynamic and Sediment Transport Modelling on Lakes: A

Preliminary Study

Rian Mantasa Salve Prastica, Herr Soeryantono, and Dwinanti Rika

Marthanty

Universitas Indonesia, Indonesia

Abstract—Lakes in Universitas Indonesia (UI) have several problems to

overcome. Water quality of UI’s lakes degrades in the last decade. One of

the problems is high turbidity. The longer turbidity in lakes, the more

dangerous the lakes environment life would be. So, this research focuses

on sediments problem or physical assessment. The main research aims to

develop hydrodynamic and sediment transport modelling of Agathis and

Mahoni Lakes in Universitas Indonesia. The authors want to increase the

quality of water in UI’s lakes and make both Agathis and Mahoni Lakes as

the representative preliminary model. Research design or methodology is

needed to concept the research to achieve goals. Unfortunately, research

methodology has not been developed yet for this case. This paper goals to

construct methodology as a preliminary study of the main research. The

authors use extensive literature review to model novel methodology.

Hydrodynamic and sediment transport modelling could be modelled by

Resources Modelling Associates (RMA). Hydrodynamic phenomena in

fluid and sediment transport modelling have the suitable governing

equation concept with RMA’s governing equation. As a thinking result, the

main research would be conducted by RMA program modelling, field

sampling, and laboratory testing. Field sampling and laboratory could

figure the amount of sediment concentration in lakes as data input. The


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final simulation of this following research is making sensitivity and

scenario analysis to prove several hypotheses.

T0009

Presentation 10

(18:35~18:50)

Color Glasses during Morning Drive for Commuting: Effects on

Autonomic Functions, Alertness, and Nocturnal Sleep

Emi Yuda, Yutaka Yoshida, and Junichiro Hayano


Abstract—Studies of illumination suggest possibility of color lights for

adjusting our mind and body to suit desired activities. To examine if

similar effects can be obtained by modulating relative contents of light

color, biological effects of color-glass wearing during early-morning drive

for commuting were studied. Compared with clear glasses, blue glasses

increased low frequency (0.04-0.15 Hz) power and deceleration capacity

and decreased respiration frequency during driving, while green, orange,

and pink glasses made no significant difference from clear glasses. None of

the color glasses showed significant difference in the performance to

psychomotor vigilance test performed at the beginning and the end of work

at job place or in the autonomic functions during sleep of that night. An

increase in the content of blue-wavelength light by blue glasses during

morning drive may attenuate sympathetic function, but it has no significant

after-effect on daytime behavioral alertness or nighttime autonomic

functions.


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Poster Session January 18, 2018 (Thursday)

Time: 13:00 ~18:25


B0004

Presentation 1

Sesamin Induces Cell Cycle Arrest and Apoptosis of Human Cervical

caRcinoma Cells Via Inhibition of PI3K/ERK and Upregulation of

p53/p21

Chun-Shiang Lin, Kuan-Te Li and Shao-Hsuan Kao

Chung Shan Medical University, Taiwan

Abstract—Cervical cancer is an important life-threaten malignancy to

women in Taiwan and the world. Recently, sesamin, a major component

in sesame seeds, has been widely explored with emphasis on antitumoral

activity against several cancer cells. Here, we aimed to explore whether

sesamin possesses antitumoral activity against human cervical cancer

cells. By using MTT assay, we found that sesamin (15 - 300 uM) lowered

cell viability of Hela and SiHa cell in a dose-dependent manner. Flow

cytometric analysis showed that sesamin increased sub-G1 ratio up to

44% (150 uM). Cell cycle regulators were determined by using

immunoblotting and the results showed that sesamin upregulated p53 and

the downstream p21, while downregulating cyclin A, cyclin B, and cdc2

in Hela cell. We further observed that sesamin inhibited survival

PI3K/ERK activation, which may attribute to upregulation of PTEN. In

addition, we also found that sesamin induced apoptotic cascades

including cleavage of caspase-9, caspase-3, and poly(ADP-ribose)

polymerase (PARP) in Hela cell, but lowered anti-apoptotic Bcl-2 level

and elevated pro-apoptotic Bax level. Collectively, these findings indicate

that sesamin can inhibit cell proliferation of human cervical cancer cells,

which may attribute to upregulation of p53 and p21, and the following

activation of apoptotic signaling and suppression of survival signaling,

contributing to G1 arrest and the further apoptosis. Accordingly, we

suggest that sesamin could be beneficial to cervical cancer treatment.

B0022

Presentation 2

The Influence of VR Color image for Color Psychotherapy

Geeyun Hong, Jiwon Lee, Cho-I Moon, Songee Kim and Onseok Lee

Soonchunhyang University, Republic of Korea

Abstract—Color therapy is a diagnostic method for treating psychological


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problems using unique vibration and frequency color. The color has

strong subjective psychology, it can give psychological stabilization

effect as a spring as a favorite color and stability become personally

personal. Also, to give various influences on emotion, we can obtain

information via brain wave to evaluate these emotions. In this research,

after viewing two kinds of VR color images of red and blue via a mobile

terminal, we used four emotional data measurable via brain waves and

grasped the user's emotional state. In addition, the stress questionnaire

was used to quantify the emotion degree, and comparative analysis was

made on how the color image affects the change of emotions with 3D

virtual reality.

B0023

Presentation 3

Segmentation for Bioluminescence Imaging of Small Animals

Cho-I Moon, Geeyun Hong, Jiwon Lee, Songee Kim and Onseok Lee


Abstract—Optical molecular imaging is less costly than other medical

imaging and is in the spotlight because of its ability to detect cancer in a

short time. However, it is not easy to automatically segment the cancer

because the resolution of the image is low. In this paper, we have

performed a study to automatically segment the region of interest in

optical molecular images using the level-based method; the

reaction-diffusion level set evolution (RDLSE) algorithm. RDLSE

showed the best performances by statistically comparing performance of

various methods with visually segmented ground truth. RDLSE is robust

to the segmentation of optical molecular images, as it compensates for the

disadvantages of other methods used in the experiments.

B0024

Presentation 4

A Study of the 3D Anatomical Structure Analysis of Bio-Sample by

AMIRA: Image Processing Application

Jiwon Lee, Cho-I Moon, Geeyun Hong, Eunyoung Kim and Onseok Lee


Abstract—In this study, we aim to obtain the fundamental data needed for

the objective analysis and bio-mimetics of leeches, using x-ray imaging

with radiation that allows 3D analysis of the detailed anatomic structures

of leeches. It was confirmed that through using radiation, detailed images

of leeches can be obtained, allowing 3D analysis of leeches’ anatomical

structures. Also, since the data obtained through the radiation allows you

to observe the micro-structure, it can serve as a good resource for

component analysis, as well as physiological and functional research.

More, it is hoped to contribute to further research in the areas of

bio-mimetics using leeches.


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B0030

Presentation 5

Protective Effects of Artocarpus Lakoocha Heartwood Extract in

UVB-Irradiated Human Keratinocytes, HaCaT Cells

Kittiya Malaniyom, Umalee Namdaung, Sunit Suksamrarn, Orapin

Wongsawatkul and Yamaratee Jaisin

Srinakharinwirot University, Thailand

Abstract—Excessive exposure to UVB radiation (220-320 nm) causes

oxidative damage to skin, resulting in sunburn, aging, and skin cancer.

Artocarpus lakoocha (A. lakoocha) heartwood extract is a rich source of

phenolic compounds conferring high antioxidant activity. The present

investigation was undertaken to determine the effects of A.lakoocha

heartwood extract in UVB- irradiated HaCaT cells. To obtain biologically

relevant information, A. lakoocha heartwood extract was subjected to

resazurin assay and antioxidant assays including DPPH scavenging assay

and griess assay. Results reveal that pretreatment the cells with

A.lakoocha heartwood extract significantly increased cell viability and

decreased nitric oxide levels in a concentration dependent manner.

A.lakoocha heartwood extract (0.25-40 g/ml) was not toxic to the cells.

Moreover, A.lakoocha heartwood extract showed a scavenging effect to

DPPH radicals. Taken together, we suggested that the protective effect of

A.lakoocha occur due mainly to antioxidant activity which provides it to

be a herbal medicine for preventing UVB-induced skin damage.

However, the further investigation through an intervening mechanism

needs to be evaluated.

B0037

Presentation 6

Flavonoids from the Roots of Scutellaria Baicalensis Possess Inhibitory

Activity against Proprotein Convertase Subtilisin/Kexin Type 9 mRNA

Expression

Young-Won Chin, Young Hee Choi, Byoung Hoon You1 and Young Mi

Kim

Dongguk University-Seoul, South Korea

Abstract—Nine flavonoids were isolated and identified from the roots of

Scutellaria baicalensis through a bioactivity-guided fractionation. This

method utilized a proprotein convertase subtilisin/kexin type 9 (PCSK9)

monitoring assay, which also identified nine known compounds from a

chloroform-soluble fraction. All structures were established by

interpreting spectroscopic data and comparing measured values from

those in the literature. All compounds were assessed for the ability to

inhibit PCSK9 mRNA expression; compounds 1

(3,7,2'-trihydroxy-5-methoxy-flavanone) and 4 (skullcapflavone II) were

found to be active. Furthermore, compound 1 was found to increase

low-density lipoprotein receptor protein expression.


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B0042

Presentation 7

Protective Effect of Limonene Against Corticosterone-Induced

Neurotoxicity

Somrudee Saiyudthong, Chantana Mekseepralard, Yamaratee Jaisin and

Punnee Nusuetrong

Srinakrarinwirot University, Thailand

Abstract—Limonene is a major aromatic compound in citrus oils which

have long been popular in aromatherapeutic practice due to reducing

physiological and psychological stress. However, its mechanism has not

been elucidated. Therefore, the aim of this study was to investigate the

protective effect of limonene on corticosterone-induced neurotoxicity in

PC12 cells. Methyl thiazolyl tetrazolium (MTT) assay, lactate

dehydrogenase (LDH) detection and flow cytometry were investigated to

confirm the neuroprotective effect of limonene against cell damage

caused by corticosterone. PC12 was treated with corticosterone 500 µM

and difference concentration of limonene (50, 100, 200, 300, 400, 500

and 600 µM) for 24 hours. Then, cells were determined the cell viability

using the MTT assay, LDH release from the cells due to corticosterone

-induced cytotoxicity and apotosis using flow cytometer assays,

respectively. Pre-incubating PC12 with 100 and 200 µM of limonene

significantly increased cell viability compared with the group treated with

corticosterone alone (p <0.01 and p <0.05, respectively). Limonene at the

concentration of 100 µM (p < 0.01) 200 µM and 300 µM (p < 0.05)

significantly reduced LDH releasing. Pre-treatment with limonene 100

and 200 µM caused higher PC12 number (p < 0.001) than those treated

with corticosterone alone. These findings indicated that limonene exerted

a neuroprotective activity against corticosterone-induced neuronal

damage. Further studies need to investigated the precise cellular and

molecular mechanisms underlying this neuroprotective activity of

limonene.

B0049

Presentation 8

Protective Effects of Beta-Mangostin against 6-OHDA-Induced

Caspase-3 Activation in SH-SY5Y Cells

Yamaratee Jaisin, Piyanee Rattanachamnong, Kittiya Malaniyom and

Sunit Suksamrarn


Abstract—Oxidative stress plays a pivotal role in Parkinson’s disease

(PD). 6-Hydroxydropamine (6-OHDA) has been widely used as a

neurotoxin to generate both in in vivo as well as in in vitro models of PD.

-Mangostin is a xanthone isolated from the pericarps of mangosteen

(Garcinia mangostana Linn), a “queen of fruit” in its native Thailand.

There is a scare study of -mangostin. We thus investigate its effect

against 6-OHDA-induced toxicity in human neuroblastoma SH-SY5Y


- 83 -

cells. Resazurin assay, ROS assay, and Caspase-3 fluorometric assay

were used to evaluate the protective effect of -mangostin on oxidative

stress-triggered neurotoxicity. It was found that after pretreatment with

-mangostin at the concentrations of 1 and 2.5 M before exposure to

6-OHDA (50 M) for 1 h; the cell viability has a significant increase and

the amount of intracellular free radicals as well as the activity of

caspase-3 were decreased. Taken together, the results of this study

indicate that -mangostin protects SH-SY5Y cells from toxic effects of

6-OHDA via its antioxidative effect. We suggest that it may have a

therapeutic potential in preventing oxidative stress which would be a

causative factor for inducing pathogenesis of PD.

B0053

Presentation 9

Wood-Plastic Biocomposites Prepared from Recycled High Density

Polyethylene Bottles and Wood Flour: A Comparative Study of Virgin

High Density Polyethylene

Nawadon Petchwattana


Abstract—The effect of wood flour (WF) contents on the mechanical,

physical, thermal and morphological properties of recycled high density

polyethylene (rHDPE) and virgin HDPE (vHDPE) and WF composites

were investigated in the current research. Both the rHDPE and vHDPE

composites were less stiff with the addition of WF. Their flexural and the

tensile strengths decreased with the WF contents. Microscopic

observations revealed some WF pulled-out particles and numerous

interfacial cavities within composites. Total water absorption at 90th day

increased with increasing WF content. In comparison, the composites

with rHDPE seemed to exhibited stiffer manner due to some in organic

additive added in the HDPE bottles. All the experimental results indicated

that recycled HDPE is possibly used as WPC matrix instead of using

virgin HDPE.

B0057

Presentation 10

Feasibility Application of Biodegradable Active Films Produced by

Poly(lactic acid) Incorporating with Limonene as An Antimicrobial

Packaging for Fresh Beef Meat

Sarinya Sangkasanya, Phisut Naknaen and Nawadon Petchwattana


Abstract—A novel composite-based packaging was prepared by blending

poly(lactic acid) (PLA) with various concentrations (0, 4, 6 and 8%, w/w)

of limonene and was applied to prolong the shelf life of fresh beef meat

during cold storage. The barrier properties of the prepared films and the

microbiological qualities of packed ground beef were evaluated. Both

water vapor permeability (WVP) and oxygen transmission rate (OTR)


- 84 -

were reduced as the contents of limonene increased. To apply the PLA

bags containing limonene to food packaging, the beef meat was packed

with the films and stored at 4oC for 12 day. The total viable count (TVB),

total coliform, Pseudomonas spp. and Staphylococcus aureus were

monitored. According to microbiological standard, the shelf life of the

control sample and the sample packed with PLA-limonene (4%) was

approximately 6 days while the samples packed with PLA-limonene at

the concentration of 6 and 8% were assured for at least 9 and 12 days of

storage, respectively. These results indicate that the PLA containing

limonene can be utilized as an antimicrobial packaging for the beef meat.

B0100

Presentation 11

Simultaneous Synthesis of Gold Nanoparticles in Dual Chamber

Microbial Fuel Cell and Their Application to Enhance Bio-Energy

Production

Md Rezaul Karim, Thi Hiep Han and Moo Hwan Cho

Yeungnam University, Republic of Korea

Abstract—In the present work, the development of Microbial fuel cells

(MFCs) was investigated by increasing performance, reducing cost and

novel application of this system. Gold nanoparticles (AuNPs) showed

highest stability among other nanoparticles and can be used as an

excellent material to modify electrode surface. In our previous work,

inexpensive three-dimensional (3D) N-doped open-porous carbon foam

anode was prepared by calcination of melamine sponge in nitrogen

environment. In this study, simultaneous bio-synthesis and decoration of

as-prepared N-doped carbon foam by AuNPs using Shewanella

oneidensis MR1 were performed. As a result, AuNPs were successfully

synthesized by S.oneidensis MR1 in MFC anode for the first time.

Moreover, the AuNPs decorated carbon foam anode produced a power

density of 46 W/m3, which was 53.3% higher than that of the bare carbon

foam anode. Cyclic voltammetry (CV) of bare and AuNPs decorated

carbon foam supported the statement that the AuNPs decorated showed

higher capacitance currents. The highly conductive AuNPs increased

surface areas, electron transfer rate along with decreased interfacial

electron transfer resistance to step-up the MFCs performance. In addition

bio-synthesize of gold nanoparticle in MFCs anode chamber is one step,

environment friendly and also an economical process.

B0101

Presentation 12

Mussel-inspired Bio-adhesive Polymer Based Drug Delivery System and

its Biomedical Application

Seung-Hyun Kim, Mira Cho and Jae-Hyung Jang

Yonsei University, Republic of Korea

Abstract—Mussels show strong adhesion properties in the dynamic sea.


- 85 -

Several previous studies demonstrated that the key element for their

adhesiveness in wet condition is the self-polymerization of catechol

functional groups in the mussel proteins and the catechol group

conjugated polymers possess the same adhesiveness. In this study, the

catechol-polymer based biocompatible and bio-adhesive drug delivery

system is used for the localized and sustained drug delivery combining

with the adeno-associated viral vector (AAV) and as a result, fast drug

adsorption and localized release of AAV were observed. Furthermore, the

physical properties (e.g. pore-size, release profiles) of this drug carrier

could be regulated for efficient therapeutic conditions. We expect that this

catechol based bio-adhesive drug delivery system could be adapted to

various drugs not only therapeutic AAV based drugs but also small

chemical and large therapeutic cells. As a result, this bio-adhesive based

drug delivery system is expected to cover a variety of biomedical

applications.

B0103

Presentation 13

Turritopsis spp. Proteins Inducing Morphological Changes of Human

Cancer Cells

Youhei Kitada, Yuki Ichii, Tatsuki Kikegawa, Kenji Etchuya, Shin

Kubota and Yuri Mukai

Meiji university, Japan

Abstract—Premature creatures including butterfly, frog and medusa,

grow up to mature individuals by repeating morphological changes. The

morphological changes are caused by cell apoptosis and differentiation

inducing factors. Detection and identification of these factors are thought

to be useful as the application of cancer therapy research and regenerative

medicine. Turritopsis spp. is one kind of medusae known as “the

immortal jellyfish” and changes itself into dumpling and polyp immatures

without dying. This phenomenon which is caused when the mature

individual approaches the time of death, is just like “rejuvenation”. In this

study, the proteins of Turritopsis spp. were analyzed to find the novel

factors for the medical treatment of human diseases.

The body fluid of Turritopsis spp. was added to the cultured human

cancer cells to confirm the interaction between the proteins of Turritopsis

spp. and human cells. As a result, almost all of the cancer cells were

changed from normal diamond shape into spherical shape. The cell

viability after morphological change was confirmed by cell staining.

Moreover, the comprehensive detection of the proteins of Turritopsis spp.

which have ability of inducing morphological changes of human cells

was conducted using "2-D Cell Blot Method" developed in our

laboratory.

B3007 Natural Dyeing of Silk Dyed with Curcuma longa Extracts Dyed in


- 86 -

Presentation 14

Yellow among Obangsaek

Jin Soun Jung

Semyung University, South Korea

Abstract— As the level of human living has improved recently and

interest in health has increased, substantial interest in well-being has

increased. Natural dyeing is a nature-friendly dyeing which is dyed with

natural materials. Therefore, it gives a lot of benefits to humans such as

the treatment of skin diseases and the insecticidal effect. Natural dyes can

be obtained in various colors depending on the dyeing method and use of

the mordant, even if the dye is taken from the same plant. In addition, the

various colors thus obtained are low in saturation, resulting in a subdued

color. Thus, the colors of fabrics obtained from natural dyeing are in

harmony with each other without special color harmony and make people

feel natural and comfortable. As each country has different cultures, color

images vary from country to country. Color image is a measure of a

society's culture and citizenship. Obangsaek is a unique color of Korean

nation. It is based on five colors of blue, red, white, black and yellow. In

this study, the natural material used for natural dyeing was Curcuma

longa which is dyed yellow, one of the five colors of Obangsaek. The

surface color, fastness, UV protection activity, deodorization, and

antimicrobial activity of the fabrics dyed with Curcuma longa extracts

was measured. The results are as follows. The color of the silk fabric

dyed with Curcuma longa extracts was 0.6Y and yellowish. Also its CIE

a* value was 7.59 and b* value was 51.34, which was slightly reddish

yellow. The V value of Munsell showing the lightness was 7.7 and the

corresponding CIE L* value was 77.92, indicating a relatively bright

color. The saturation C which indicates the degree of clearness of the

color was 7.9, indicating a medium saturation. Its dry cleaning fastness

was excellent in grades 4 to 5, including discoloration. Its perspiration

fastness was high in grade 4, both in acidity and alkalinity. In the case of

rubbing fastness, both the dry state and the wet state were excellent in 4

grade. The light fastness was 1 grade and lower than other fastness. The

sun protection factor was 5.4, indicating no UV protection effect. And It

showed 99.9% of antimicrobial activity on staphylococcus aureus ATCC

653B and Klebsiella pneumoniae ATCC 4352. Therefore, it was found

that it has a high antimicrobial activity on the two bacteria.

B3008

Presentation 15

Influence of Potato Virus YO in Autophagy of Nicotiana Benthamiana

Hyeonsook Cheong, Jaeyoung Park and Wonjin Kim

Chosun University, Korea

Abstract—Autophagy plays a critical role in the innate immune response

of plants to pathogen infection. In the present study, we examined


- 87 -

autophagy induced by PVYO infection in Nicotiana benthamiana. ELISA

revealed that the number of virus particles in the plant peaked at 2 weeks

gradually decreased. Also, PVYO increased in the 3rd and 4th leaves and

decreased in the 5th leaf. Moreover, PVYO infection caused changes in

the number, size, and shape of chloroplasts, whereas the number of

plastogranules increased. Furthermore, double-membrane

autophagosome-like vacuoles, including electron-dense materials,

laminated structures, and cellular organelles, were found. The induction

of autophagy after the PVYO infection of leaves was further confirmed by

the expression of lipidated microtubule-associated LC3-II, an autophagy

marker and p62, an autophagy adaptor protein. The LC3-II levels

increased daily over the 4-week period. Although virus inoculation was

performed on the basal leaves, LC3-II was expressed throughout the

leaves and the expression was higher in leaves distal to the inoculated

leaf. PVYO infection caused the activation of stress-activated protein

kinases/c-Jun N-terminal kinases. Therefore, PVYO infection-induced

autophagy was positively correlated with the virus content, suggesting

that autophagy induction following PVYO infection is involved in the

antipathogen response of the host.

B3009

Presentation 16

Regulation of Adipocyte Gene Expression and Differentiation by

Funtional Plant P. densiflora

Woong Kim, Jaeyoung Park, Beomgi Lee, Cheolwoo Park, Wonjin Kim

and Hyeonsook Cheong


Abstract—Obesity is a common disease in worldwide and considered a

significant risk factor causing various complications. Pinus densiflora

sieb. et zucc contains several natural compounds that have various

biological activities. However, the health beneficial effects of these

compounds have rarely been reported. In this study, to evaluate the

inhibitory effect of pine needle extract powder(PNEP) on adipocyte

differentiation in 3T3-L1 preadipocytes. The level of adipogenesis in the

3T3-L1 cells was measured by Oil Red O staining. As a result of Oil Red

O staining, PNEP significantly inhibited adipocyte differentiation by

more than 55% in 3T3-L1 preadipocytes in a dose-dependent manner.

Furthermore, We confirmed PNEP inhibited adipocyte differentiation by

suppressing the expression of the adipogenic transcription factors Fatty

acid binding protein(aP2) and Sterol regulatory element binding

transcription factor 1(SREBP1c) using real-time PCR analysis. These

results shows that PNEP inhibits adipogenesis by suppressing the

expression of adipogenic transcription factors.

B3010 Bioactivity of UVB Induced Skin Inflammation Factor in HaCaT Cells by

Needle Extract of P. densiflora


- 88 -

Presentation 17

Cheolwoo Park, Woong kim, Jaeyoung Park, Beomgi Lee, Wonjin Kim

and Hyeonsook Cheong


Abstract—Ultraviolet B (UVB) irradiation is known to induce

inflammation, oxygen stress, skin cancer and photo-aging. UVB

irradiation generates intracellular reactive oxygen species (ROS), and

induced matrix metalloproteinase-1 (MMP-1) and pro-colleagenase.

Pinus densiflora Sieb. et Zucc. (red pine) has various biological activities

such as antimicrobial and antioxidant. However, the skin

anti-inflammation effects of red pine have rarely been reported. In this

study, we partitioned n-Hexane, EtOAc n-BuOH and H2O layer from

methanolic extract of red pine needles and tested anti-inflammation

activity on . As a result, Inhibition of TNF-α induced IL-1β expression by

n-BuOH layer than other layers. We check up on the effects of n-BuOH

layer on inflammation, photo-aging and investigated its mechanism of

action in human keratinocytes HaCaT cells. HaCaT Cells were then

exposed to UVB light (15 mJ/㎠). DCF-DA assay revealed that n-BuOH

layer (25-100 ug/mL) inhibited intracellular ROS. Furthermore, MMP-1

level, which has been known to be induced by ROS, was significantly

reduced by n-BuOH layer (25-100 ug/mL) on Real-Time PCR. Moreover,

UVB induced pro-inflammatory cytokine factors(IL-6, IL-8, TNF-a and

COX-2) were significantly suppressed by n-BuOH layer (25-100 ug/mL)

in a dose-dependent manner. Chromatography purification and

fractionation of n-BuOH layer were conducted to prove suppression

UVB-induced ROS, MMP-1 and inflammation factor level.

T0007

Presentation 18

Alcohol Drinking Strengthens the Association between Ambient Air

Pollution and Liver Enzymes

Hyun-Jin Kim, Jin-young Min, Yong-Seok Seo, and Kyoung-bok Min

Seoul National University, Korea

Abstract—Some studies have found a link between air pollutant exposure

and liver enzyme levels in restricted areas or elderly people, but the

available data cannot generalize. This nationwide study of South Korea

adults investigated the association between air pollution, liver enzyme

levels, and alcohol consumption. The annual average concentrations of

the air pollutants including particulate matter with an aerodynamic

diameter £10 mm (PM10), nitrogen dioxide (NO2), sulfur dioxide (SO2),

and carbon monoxide (CO) were calculated. Liver enzymes included

alanine aminotransferase (ALT) and aspartate aminotransferase (AST).

The PM10, NO2, SO2, and CO were found to be significantly associated

with elevated ALT and AST levels, with increase ranged from

0.0073IU/L(95% CI =0.0042, 0.0104) to 0.0251 IU/L(95% CI =0.0132,


- 89 -

0.0371) per IQR increase of each pollutant(all p< 0.001). Innon-drinkers,

the PM10and CO exposures were not related to the ALT and AST levels

(all p> 0.05), whereas, in drinkers, significant associations between these

pollutants exposures and liver enzymes were observed (all p<

0.05).Chronic exposure to air pollution, especially PM10and CO, is

associated with an increase in liver enzyme levels, depending on alcohol

consumption, in the general adult population.

B0001

Presentation 19

The Effects of the Ethanol Extract from the Leaves of Malachra Capitate

L. and Lantana Camara L. on Mortality and Surface Structure of the

Culex Larvae

Saminee Suksumek, Weerawan Wonpuean, Patarapong Kroeksakul,

Phanom Sutthisaksopon and Arin Ngamniyom


Abstract—The Culex spp. are the vector-borne diseases that are widely

distributing throughout tropical countries including Thailand. These

mosquitoes are usually controlled and against by using insecticides.

However, it is concerned the pollutions of contaminations of those

chemicals in the environments leading to the negative impacts of the

human and animal health. Malachra capitate and Lantana camara are the

invasive weeds that are found in every region of Thailand. However, the

utilizations of those weeds for insect control have been evaluated.

Therefore, this study aims to investigate the effects of crude extracts from

the leaves of M. capitate and L. camara to the morality, mobility and

integuments of Culex larvae using a scanning electron microscopic

(SEM) and histological observation. In morality, 60% of lethal values and

abnormal mobility were found in the treatment of L. camara at 1 mg/L

for 24 hr. In SEM and histological analysis, the erosions and shrink of

integument and damaged cuticle layers were observed at 10 mg/L of M.

capitates, and 0.1 and 1 mg/L of L. camara crude extracts for 24 hr.

These results suggest that the crude extracts of L. camara may affect on

the morality, mobility and surface morphologies of Culex larvae.

B0109

Presentation 20

Neural Network-Based Discrimination of Golgi Type II Membrane

Proteins with Better Accuracy

Tatsuki Kikegawa, Kenji Etchuya and Yuri Mukai


Abstract—Type II membrane proteins in the Golgi apparatus play

important roles in biological functions, and predominantly exist as

catalysts related to post-translational sugar modification. This study

describes a new method for detecting Golgi-localized type II membrane

proteins (GLs) from post-Golgi type II membrane proteins (PGs), which


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are mainly localized in the plasma membrane and endoplasmic reticulum

(ER). The method is based on hydropathy profiles and the

position-specific scoring matrix (PSSM) in combination with the back

propagation artificial neural network (BP-ANN). The accuracy of

discriminating GLs from PGs was evaluated in a 5-fold cross-validation

test with 94.7% sensitivity and 93.5% specificity. This result shows that

our method can predict GLs with high accuracy, and that the PSSM and

BP-ANN

B0111

Presentation 21

Biological Network Analysis of Synthetic Pathogenicity Genes of

Atherosclerosis

Jhih-Ying Chen, Chia-Min Chen and Pei-Chun Chang

ASIA University, Taiwan

Abstract—Atherosclerosis is a chronic inflammatory process. In general,

it proceeded repeatedly to form a layer of plaque. Atherosclerotic plaques

are separated into two broad categories: stable and unstable. Stable

atherosclerotic plaque tends to be asymptomatic. Unstable plaques are

rich in macrophages and the extracellular matrix may separate from the

arterial lumen into circulation and eventually induce thrombus formation

in the lumen. This catastrophic event is called an infarction. One of the

most common recognized scenarios is called coronary thrombosis of

coronary artery, causing myocardial infarction. Therefore, early diagnosis

and administration of antithrombotic drugs is crucial to preventing

atherosclerosis, especially for unstable type of atherosclerotic lesions.

Synthetic lethality arises when a combination of mutations (or differential

expression) in two genes leads to cell death, whereas a mutation in only

one of these genes does not. We proposed a notion of synthetic

pathogenicity. Synthetic pathogenicity arises when a combination of

mutations (or differential expression) in two genes leads to disease,

whereas a mutation in only one of these genes does not. We isolate those

genes which locate on the hub with high connections in the network of

synthetic pathogenicity genes. These genes may be the drug targets for

Anti-thrombosis.

Dinner

19:00-20:30 Lounge in Front of C5


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Conference Venue

Global Front, Surugadai Campus, Meiji University, Tokyo, Japan

http://www.meiji.ac.jp/cip/english/about/campus/surugadai.html

Surugadai Campus is the traditional home to Meiji University. The campus is located in the

Kanda Surugadai area which, while retaining a strong atmosphere of a students’ town, is

located in proximity to Kasumigaseki, Otemachi, and other areas that are home to companies

and government ministries and agencies that are pivotal to Japan’s politics and economy. The

campus houses various educational and research facilities centering around the Liberty

Tower, which has become a landmark, and the Academy Common. In January 2013, the

Global Front was completed, where liberal arts graduate schools, research facilities,

Organization for the Strategic Coordination of Research and Intellectual Properties, and

Organization for International Collaboration are gathered under the concept of “a

state-of-the-art research cultivation hub that transmits to the world.”


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Map of Global Front and University Hall


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One Day Visit & Tour 09:30~17:30, January 20, 2018 (Saturday)

Assemble at APA Hotel Kanda Jimbocho Ekihigashi:09:30 — Visit Meiji University

Museum:10:00~11:00—Have Lunch in Local Restaurant:11:00~12:00—City Tour to Sensoji

Temple, Tokyo Sky Tree, The Tsukiji Market, and Odaiba Marin Park:12:30~17:30—Go Back

to APA Hotel Kanda Jimbocho Ekihigashi:17:30.

Based on the University's founding philosophy of "Rights, Liberty,

Independence and Self-Government", the Meiji University Museum

aims to publicly exhibit the achievements of its academic pursuits and to

also serve as an institution of learning. The Museum is divided into three

departments: The Commodity Department, the Criminal Materials

Department and the Archaeology Department.

Senso-ji Temple is located in Taito Ward, Tokyo, is Japan's existing

with "Edo style" of the public recreational land. According to legend,

the Empress Suiko Sanshiliunian, there are two fishermen fishing in

the Palace Togawa, picked up a gold one, 5.5 cm of Avalokitesvara, the

vicinity of people built a temple to raise funds dedicated to the Buddha

statues, and this is Senso-ji Temple.

Tokyo Sky Tree is a radio tower located in Sumida, Tokyo, Japan. The height

of 634.0 meters, in November 17, 2011 Guinness World Records certified as

"the world's tallest tower", becoming the tallest self-standing radio tower in

the world. It is also the second tallest building in the world after Burj Khalifa

in Dubai.

The Tsukiji Market is said that "Uogashi" or a riverside fish market

dates back to the 16th century, the beginning of the Edo period.

Tokugawa Ieyasu, the first Tokugawa shogun and builder of Edo as is

now Tokyo, invited fishermen from Tsukudajima, Osaka and gave them

a privilege for fishing in order to let them supply seafood to Edo Castle.

The fishermen purveyed fish to the Castle and sold the remains near the

Nihonbashi bridge. It was the origin of Uogashi.

Odaiba Marin Park is an artificial seaside park that can enjoy the

views of the Tokyo coastline. Swimming is prohibited here, but it’s

available for visitors play on the coast and reef, you can also enjoy the

windsurfing, overlooking the Rainbow Bridge across the street and

other beautiful scenery.

Notice: One lunch during the tour is covered, but the tour does not cover the entrance tickets. This

arrangement is temporary, The final agenda should be based on the actual one declared on the tour day.


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Note


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