Proposal #: 15-05

2013 FASEB SCIENCE RESEARCH CONFERENCES ADVISORY COMMITTEE MEETING

TOPIC FOR CONSIDERATION TOPIC NAME: GASTROINTESTINAL TRACT XVI. GI HOMEOSTASIS: THE MICROBIOME AND THE

BARRIER, DEVELOPMENT AND DISEASE PREVIOUS TITLE: Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer SUBMITTED BY: Richard M. Peek, Jr., Vanderbilt University Medical Center Jason Mills, Washington University School of Medicine Melissa H. Wong, Oregon Health & Science University YEAR REQUESTED FOR

SCHEDULING: 2015

SITE REQUESTS: 1. Steamboat Springs, CO

2. Big Sky, MT 3. Keystone, CO

DATE REQUESTS: 1. August 9-14, 2015

2. August 16-21, 2015 3. August 2-7, 2015

YEAR(S) CONFERENCE

HAS BEEN HELD: 1985, 1987, 1989, 1991, 1993, 1995, 1997, 1999, 2001, 2003, 2005, 2007, 2009, 2011, 2013

NOTES: To the best of our knowledge, there is not a direct conflict with any other

FASEB SRC or other society or industry meeting.

2015 FASEB Summer Research Conference Proposal

Gastrointestinal Tract XVI. GI homeostasis: The microbiome and the barrier, development and disease

Section 2: Conference Title & Organizer Information: TITLE OF CONFERENCE: Gastrointestinal Tract XVI. GI homeostasis: The microbiome and the barrier, development and disease # of EXPECTED ATTENDEES: 160 Preferred Primary Point of Contact for Proposal Questions: Dr. Richard M. Peek, Jr. Professor of Medicine and Cancer Biology Director, Division of Gastroenterology, Hepatology and Nutrition Vanderbilt University Medical Center 2215 Garland Avenue, 1030C MRB IV Nashville, TN 37232, USA Phone: 615-322-5200 Fax: 615-343-6229 Email: richard.peek@vanderbilt.edu Science Categories

1) Cell Biology, 2) Developmental Biology, 3) Microbiology Organizing Committee: a) Organizer Co-Chair: Richard M Peek, Jr., MD Professor of Medicine and Cancer Biology Director, Division of Gastroenterology, Hepatology and Nutrition Vanderbilt University Medical Center 2215 Garland Avenue, 1030C MRB IV Nashville, TN 37232, USA Phone: 615-322-5200 Fax: 615-343-6229 Email: richard.peek@vanderbilt.edu b) Organizer Co-Chair: Jason Mills, MD/PhD Associate Professor of Medicine Associate Director, Washington University Digestive Disease Center Washington University School of Medicine 660 S. Euclid Avenue, Campus Box 8124 St. Louis, MO 63110, USA Phone: 314-362-4213 Fax: 314-632-7487 Email: jmills@wustl.edu c) Organizer Co-Chair: Melissa H. Wong, PhD Associate Professor Vice Chair of Cell and Developmental Biology Oregon Health & Science University 3181 SW Sam Jackson Park Rd, Mailcode L215 Portland, OR 97239, USA Phone: 503-494-8749 Fax: 503-494-4253 Email: wongme@ohsu.edu

Section 3: Program Submission Requirements & Outline:

Overview of Program Flow # of Days: 6 # of Sessions per day: 2 # of Speaker per day: 10-18 # of Session chairs per day: 2-4 # of Breakouts per day: 1 breakout session/meeting (Meet the Experts) # of Abstracts per day: 3-4 Abbreviation Key: C = confirmed chair; CS = confirmed speaker; W = female; M = minority; NEW = new speaker or chair; ASSTP = assistant professor; *NYI = not yet invited *Note that we have opted to allow for some flexibility in the scheduling to accommodate the inclusion of invited talks that represent newly emerging science in 2013-2015. SUNDAY To include: Conference Registration 4 p.m. – 9 p.m., FASEB SRC Welcome Reception 5 p.m. – 6 p.m., Dinner 6 p.m. – 7 p.m. Title of Session: Keynote Session Chair & Affiliation: Richard Peek, Vanderbilt (C), Jason Mills, Washington University School of Medicine (C), Melissa Wong, Oregon Health & Science University (C, W) Martin Blaser, MD (CS, NEW) New York University School of Medicine Keynote: "The human microbiome: at the interface of health and disease" MONDAY To include: Group photo during morning break 1) Title of Session: Microbial Ecosystems and the GI Tract Session Chair & Affiliation: Gail Hecht, Loyola University (C, W) Speaker 1: Andrew Neish, Emory University (CS, NEW) Tentative Title of Talk: “Redox signaling and the gut microbiota” Speaker 2: Karen Guillemin, University of Oregon (CS, W, NEW) Tentative Title of Talk: “Zebrafish as a model to study bacterial-host symbiosis” Speaker 3: Lora Hooper, University of Texas-Southwestern (CS, W) Tentative Title of Talk: ”Interactions between the microbiota and the immune system” Speaker 4: Justin Sonnenburg, Stanford University (NYI, NEW, ASSTP)

Tentative Title of Talk: “Bacterial consumption of host glycans and gut pathology” Speaker 5: Sarkis Mazmanian, California Institute of Technology (NYI, NEW) Tentative Title of Talk: “Disruption of the gut microbiome and microbial infections” E poster session Moderator & Affiliation: Wayne Lencer, Dana Farber/Harvard (NYI) 6 ePoster presentations selected from junior faculty and trainee abstracts Breakout session: Meet the experts Panel & Affiliation: Judy Podskalny, NIH (NYI, W), Jill Carrington, NIH (NYI, W), Michael Grey, NIH (NYI, NEW), Sean Colgan, University of Colorado (NYI) Poster session 2) Title of Session: Host and microbial mediators of disease Session Chair & Affiliation: James Casanova, University of Virginia (C) Speaker 1: Nina Salama, University of Washington (CS, W, NEW) Tentative Title of Talk: “Novel pathogenic functions for bacterial cell wall constituents” Speaker 2: Brent Polk, University of Southern California (CS, NEW) Tentative Title of Talk: “The duality of EGFR in inflammation-associated cancer” Speaker 3: Beth McCormick, University of Massachusetts (CS, W) Tentative Title of Talk: “Salmonella effector proteins and host cell responses” Speaker 4: Thad Stappenback, Washington University (NYI, NEW) Tentative Title of Talk: “Viral interactions with the host and intestinal injury” TUESDAY 3) Title of Session: Mucosal Immunity Session Chair & Affiliation: Cormac Taylor, University College Dublin (C) Speaker 1: Rodney Newberry, Washington University School of Medicine (CS, NEW) Tentative Title of Talk: “Antigen presentation across the gut epithelium” Speaker 2: Ruslan Medzhitov, Yale University (CS, NEW) Tentative Title of Talk: “Innate immunity, microbes, and the gut” Speaker 3: Richard Di Paolo, St. Louis University (CS, NEW, ASSTP) Tentative Title of Talk: “Cytokines and metaplasia in the stomach” Speaker 4: Ken Cadwell, New York University (NYI, NEW, ASSTP) Tentative Title of Talk: “Autophagy and gut microbes” Speaker 5: Gabriel Nuñez, University of Michigan (NYI, M, NEW) Tentative Title of Talk: “Nod-like and Toll-like receptors in inflammatory bowel disease”

E poster session Moderator & Affiliation: Deborah Gumucio, University of Michigan (NYI, W) 6 ePoster presentations selected from junior faculty and trainee abstracts Poster session 4) Title of Session: Transport, tight junctions and barrier functions Session Chair & Affiliation: Asma Nusrat, Emory University (C, W) Speaker 1: Mark Donowitz, Johns Hopkins University (CS, NEW) Tentative Title of Talk: “Transport, barrier maintenance and pathogens” Speaker 2: Jerrold Turner, University of Chicago (CS) Tentative Title of Talk: “Cytokines, cytoskeleton, tight junctions and the barrier” Speaker 3: Mark Frey, University of Southern California (CS, NEW, ASSTP) Tentative Title of Talk: “Growth factors and epithelial barrier function” Speaker 4: Kim Barrett, University of California, San Diego (NYI, W, NEW) Tentative Title of Talk: “Transport, growth factors and diarrhea” Speaker 5: Declan McCole, University of California, Riverside (NYI, NEW, ASSTP) Tentative Title of Talk: “Regulation of barrier function by cytokines” WEDNESDAY 5) Title of Session: GI Stem Cells Session Chair & Affiliation: Susan Henning, University of North Carolina (C, W) Speaker 1: Calvin Kuo, Stanford University (CS, NEW) Tentative Title of Talk:”Intestinal stem cell hierarchy” Speaker 2: Linheng Li, Stowers (CS, NEW) Tentative Title of Talk: “Regulatory niche of the intestinal stem cell” Speaker 3: Craig Micchelli, Washington University School of Medicine (CS, NEW, ASSTP) Tentative Title of Talk: “Quiescent gastric stem cells in the Drosophila intestine” Speaker 4: Nick Barker, Institute of Medical Biology, Singapore (NYI, NEW, ASSTP) Tentative Title of Talk: “Lgr5 stem cells in regeneration and cancer” Speaker 5: Volker Harenstein, UCLA (NYI, NEW) Tentative Title of Talk: “Migration of small intestinal stem cells”

2 short presentations selected from junior faculty and trainee abstracts

Group activity 1 p.m. – 5 p.m. 6) Title of Session: Dedifferentiation and reprogramming in development and cancer Session Chair & Affiliation: Linda Samuelson, University of Michigan (C, W)

Speaker 1: Owen Sansom, Beatson Institute, Glasgow, Scotland (CS, NEW) Tentative Title of Talk: “Early events in intestinal neoplasia” Speaker 2: Meri Huch, University of Heidelberg, Germany (CS, W, NEW, ASSTP) Tentative Title of Talk: “Stem cell plasticity in the stomach and other GI organs” Speaker 3: Steven Leach, Johns Hopkins University (CS, NEW) Tentative Title of Talk: “Differentiation, dedifferentiation, transcription factors and pancreatic acinar cells” Speaker 4: Courtney Houchen, Oklahoma University Health Sciences Center (NYI, M) Tentative Title of Talk: “Dedifferentiation, DLSCK1 and cancer in intestine and pancreas” Speaker 5: Florian Greten, University of Frankfurt, Germany (NYI, NEW) Tentative Title of Talk: “Reprogramming of differentiated cells in cancer” THURSDAY 7) Epithelial injury, regeneration and disease Session Chair & Affiliation: Kay Lund, University of North Carolina (C, W) Speaker 1: Noah Shroyer, Baylor College of Medicine (CS) Tentative Title of Talk: ”Notch signaling regulation in cancer” Speaker 2: Tracy Grikschiet, UCLA (CS, W, NEW, ASSTP) Tentative Title of Talk: ”Bioengineering the intestine” Speaker 3: W. James Nelson, Stanford (NYI) Tentative Title of Talk: ”Cell adhesion impact on regeneration and disease” Speaker 4: Eric Fearon, University of Michigan (NYI, NEW) Tentative Title of Talk: “Cell signaling regulation in homeostasis and disease” Speaker 5: Juanita Merchant, University of Michigan (NYI, W, M, NEW) Tentative Title of Talk: “Gastric disease and regeneration” 2 short presentations selected from junior faculty and trainee abstracts Group activity 1 p.m. – 5 p.m. 8) Title of Session: Cutting edge techniques/concepts in GI research Session Chair & Affiliation: Manuel Amieva, Stanford University (C, M) Speaker 1: James Wells, University of Cincinnati (CS, NEW) Tentative Title of Talk: ”iPS cells in translational medicine” Speaker 2: Scott Magness, University of North Carolina (CS, ASSTP) Tentative Title of Talk: “Manipulating the intestine ex vivo with gut culture” Speaker 3: Robert Coffey, Vanderbilt University (CS) Tentative Title of Talk: “Exosomes and the GI tract” Speaker 4: Ken Lau, Vanderbilt University (CS, NEW, ASSTP) Tentative Title of Talk: “4D-imaging of the intestine”

FRIDAY Breakfast, departures at 9 a.m.

End of Conference

Section 4: Content Assessment: Please complete the grid below which will assist the FASEB SRC Advisory Committee in assessing the requirements of the proposal. Positive reviews are given to proposals with confirmation of session chairs and invited speakers, new speakers to the program, a good representation of women, and a sufficient number of short talks from junior level investigators. Indicate the number of session chairs that have confirmed their participation: 11/11 Indicated the number of women included with the entire program: 17 Indicate the number of session chairs/speakers of a minority group: 4/39 Indicate the number of new speakers to the Conference: 32/39 Indicate the number of speakers that have confirmed their participation in the Conference: 25/39 Indicated the number of talks set aside for junior level investigators to present their work: 15/43 Indicated the number of poster sessions that will be organized: 2 Please provide a brief description of the how the poster sessions will be organized: We will introduce an ePoster presentation session that will highlight 6 posters/session from the abstracts of junior faculty/trainees. This will consist of a 5 minute oral presentation of the poster in the general session. The traditional poster session will be split into two days and will be thematically grouped. A panel of judges will preview the posters and select posters of note for award recognition. Indicate (if known) if there is a potential of a conflict with any other FASEB SRC or any other society or industry meeting. If yes, please explain the conflict in detail. We do not believe that there is a direct conflict with any other FASEB SRC or other society or industry meetings. We discussed a potential conflict with the newly formed Gordon Research Conference mucosal stem cell meeting that was held in July 2013 in the narrative, and note that the organizers of the Gordon Conference have agreed to move the conference to even calendar years to avoid conflict with our established meeting.

Section 5: Scheduling & Location Preferences:

Select three (3) choices of dates that you wish to hold the Conference. Define the pattern flow, start date, and end date of your Conference Week 1: August 9-14, 2015 Week 2: August 16-21, 2015 Week 3: August 2-7, 2015 Preferred venue: Hotel

Cities/venues Preference Steamboat Springs, CO 1 2 3 Big Sky, MT 1 2 3 Keystone, CO 1 2 3

Biographical Sketches

Richard Peek, MD (Co-organizer)

Jason Mills, MD/PhD (Co-organizer)

Melissa Wong, PhD (Co-organizer)

Table 1. Previous gastrointestinal tract summer conference data.

Past Conference Details 1985 Chair: Lenard Lichtenberger, Univ. of Texas

Vice-chair: Susan Henning, Univ. of Houston 1987 Chair: Leonard Johnson, Univ. of Texas HSC

Vice-chair: James Jamieson, Yale Univ.

1989 Chair: Jackie D. Wood, Ohio State Univ. Vice-chair: Gilbert Castro, Univ. of Texas HSC

1991 Chair: Mike Gershon, Columbia Univ. Vice-chair: Marion Neutra, Harvard Univ.

1993 Copper Mountain, Colorado Chair: James L. Madara, Harvard Med. Sch.

Vice-chair: David H. Alpers, Washington Univ. 1995 Snowmass Village, Colorado Chair: Mark Donowitz, John Hopkins Univ. Vice-chair: Jeffrey Gordon, Washington Univ. 1997 Copper Mountain, Colorado Chair: Peter G. Traber, Univ. of Pennsylvania Vice-chair: W. James Nelson, Stanford Univ. 1999 Copper Mountain, Colorado Chair: Kim Barrett, Univ. of California/San Diego Vice-chair: Daniel Louvard, Institut Curie, Paris 2001 Whitefish, Montana Chair: Gail Hecht, Univ. of Illinois, Chicago Vice-chair: James Anderson, Yale University 2003 Tucson, Arizona Chair: James Goldenring, Vanderbilt University Vice-chair: Jeffrey Matthews, University of Cincinnati 2005 Snowmass, Colorado Chair: Marshall Montrose, University of Cincinnati Vice-chairs: Jerrold Turner, University of Chicago

Vincent Yang, Emory University 2007 Chair: Brent Polk, Vanderbilt University Vice Chairs: Juanita Merchant, University of Michigan

Beth McCormick, Harvard University

2009 Chair: Shanthi Sitaraman, Emory University Vice Chairs: Cormac Taylor, University College Dublin

Wayne Lencer, Harvard University

2011 Steamboat Springs, CO Chair: Sean Colgan, University of Colorado Vice Chairs: Linda Samuelson, University of Michigan Gary Wu, University of Pennsylvania 2013 Steamboat Springs, CO Chair: Asma Nusrat, Emory Vice Chairs: Robert Coffey, Vanderbilt Thaddeus Stappenbeck, Washington University School of Medicine

Section 6: Justification

Information for the Summer Conference Advisory Committee 1. Topic of high current interest: This grant proposal is the sixteenth in a series of highly successful meetings that have been focused on the gastrointestinal tract and have been held biennially since 1985. The need for another conference on this topic was agreed upon by the unanimous vote of attendees at the last meeting, held August 11-16, 2013 at Steamboat Springs, Colorado. Research pertaining to the pathobiology, pathology and physiology of the gastrointestinal tract is progressing at such a rapid pace that the scientific community working in these areas view the format of this conference as the best venue for acquiring the most current scientific information and in establishing collaborations. Additionally, this conference provides an ideal forum for active participation of trainees (graduate student, postdoctoral fellows) and junior faculty that is vital not only for their career advancement, but also for the future of cutting edge science in this field. 2. Rapidly growing field: This topic remains a rapidly growing field of interest because it combines research endeavors that impact not only on gastrointestinal pathophysiology, but also on general aspects of gastrointestinal epithelial biology including tight junctions and transport proteins, host-microbial relationships, mucosal inflammation and immune response, stem cells, development, and neoplasia. Indeed, this meeting is widely acknowledged by investigators working in the gastrointestinal sciences as the premier scientific conference in the field. For the 2015 meeting, the substantial majority of speakers have been invited to introduce diverse and novel topic areas that cover both conceptual and technological advances that have not been previously presented. The invited chairs are primarily investigators who have participated regularly in the meeting and traditionally bring trainees from their laboratories, which will maintain the high levels of attendance that the meeting has enjoyed over its long history. 3. Previous conferences: Since the inception of this meeting (1985), this series of conferences has been held on a biennial basis in Colorado, Montana or Arizona. In discussing these issues at the most recent meeting in 2013, all attendees agreed that a meeting in 2015 is an appropriate interval for the emergence of new knowledge to ensure a stimulating and productive meeting. We therefore propose that the meeting be held in 2015 with the order of preference, from first to last, for the weeks of August 9-14 (second choice: August 16-21, 2015; third choice: August 2-7, 2015). Based on the past attendee experiences at different FASEB sites, the participants had a strong preference for the meeting to be held at either Steamboat Springs, Colorado (1st choice) or Big Sky, Montana (2nd choice). There was a general concern pertaining to Snowmass and Tucson locations due to the physical commute to these sites, conference room logistics/location, limited social activities for investigators to interact, and uncomfortable climate in mid-summer (Tucson). There was also general dissatisfaction with Saxon River from attendees who had been there in the recent past. Therefore, based on the recommendation of attendees at the last meeting, we respectfully request that the proposed meeting in 2015 be held in Steamboat Springs, Colorado. 4. How many participants: The proposed conference would be the sixteenth edition of a meeting that has been held biennially since 1985. An attached Table 1 summarizes information from these previous meetings. The last conference was held in 2013 at Steamboat Springs, Colorado. Previous meetings in this series have attracted over 100 attendees. The 2013 conference had maximal attendance, with 160 attendees. Our expectation is that we will meet and surpass the 2013 attendance level in the 2015 meeting, especially at a favorable site. We

are not aware of any other meeting whose content would potentially conflict with this conference in 2013. It is of note that the Gordon Research Conference on Mucosal Stem Cells has moved their cycle to opposite years of this FASEB meeting to avoid conflict. The preliminary program has been established in consultation with the session chairs who have all agreed formally to participate. In addition to individuals invited as speakers and/or session chairs, we will specifically invite attendees from the last three meetings held on this topic. Directed invitations will be sent to chairs in Pathology, Physiology and Cell Biology as well Chiefs of Gastroenterology and Surgery at institutions with a strong research emphasis requesting that information concerning this meeting be disseminated to research faculty, graduate students, postdoctoral fellows, and residents. 5. Percentage of women and junior investigators: The conference has had an exceptional record of involving both women and junior investigators as invited participants. In 2013, 14 of the 29 invited speakers were women, and five of the nine session chairs were women. Over ninety percent of the invited speakers had not presented at the previous two meetings. For 2015, one of the three organizers is a woman (Dr. Wong) and we will attempt to recruit at least 40% women as speakers or session moderators. Thus far 33% of our confirmed invited speakers and session moderators are women. 6. Recruitment of junior investigators. Active participation of junior investigators will be encouraged by the provision of travel grants to those graduate students, postdoctoral fellows and junior faculty who present talks or posters. In the interest of career development for trainees and junior faculty, analogous to the 2011 meeting, we plan to invite Drs. Judith Podskalny and Jill L. Carrington who are program officers at NIDDK for career development awards and R01’s respectively, to hold a panel discussion (Meet the Experts) on grant writing and logistics of transitioning from career development awards to independent R01 grants. Note that they were unable to attend the 2013 meeting, but we have already had discussions with both Dr. Carrington and Dr. Podskalny regarding the importance of the NIDDK at meetings that encourage attendance of post-doctoral fellows and junior investigators, and they have acknowledged the importance of an NIH perspective in this meeting. We will also invite Dr. Michael Grey from NIDDK to discuss translational research opportunities available at NIH as well as Dr. Sean Colgan, previous Chair of the Gastrointestinal Mucosal Pathobiology Study Section to provide important insights into grant preparation and review. We have included short talk slots for 2 trainees to present their work orally in three of the sessions and plan to expand the number of trainee presentations by selecting 8-12 abstracts for ePoster presentations preceding the formal poster presentations. The ePoster format will involve a short poster-driven presentation of the work in a 5-minute talk in front of the assembled attendees. We anticipate that this format will facilitate more in depth and one-on-one discussions with the trainee during the formal poster session and throughout the duration of the meeting. We anticipate that these three formats will encourage scientists and junior investigators to attend the meeting and include their trainees, which we believe will provide an added strength and additional numbers for the 2015 conference. In keeping with the successful 2013 meeting format, we will have a social event around each poster session (including food and drink), and plan to award poster prizes for top posters if funds allow, as initiated during the 2013 conference. Thus, in summary, we will continue in the tradition of the 2013 meeting that included significant participation of junior scientists in addition to established investigators. This format will therefore allow for maximum active participation of the entire research community in gastrointestinal pathobiology, physiology and therapeutics. 7. Recruitment of new speakers: We have committed to a strong recruitment of new speakers to the program, while retaining past speakers at the cutting edge of gastrointestinal research. In

fact, 82% of the invited speakers will not have spoken in the past two meetings of this conference and 28% of invited speakers are currently assistant professors. Both the proposed topics and speakers have been vetted with past attendees and current session chairs to assure a high degree of interest, continued support, and likely attendance. Ninety-four percent of the 2013 conference attendees reported that they intend to attend the 2015 meeting. 8. Who will attend this conference: From previous conference attendance (Table 1), we predict that there will be strong attendance from three different areas of GI research: mucosal immunology and microbiota, stem cells and differentiation, and cell adhesion. Further, this conference has been supported by a range of established investigators, new investigators and trainees. We plan to encourage trainees and new investigators to attend this meeting by offering travel awards and a career development session. As always, the small numbers (we anticipate ~160 attendees) of investigators provide an intimate exchange of science and encourage scientific discussion between scientists at all stages. In the past, the conference has been well attended by both PhD, MD, and MD/PhD scientists. 9. Solicitation of funds: Financial support for the meeting will be sought from the National Institute of Diabetes Digestive and Kidney Disorders (NIDDK), the American Digestive Health Foundation (the funding body associated with American Gastroenterological Association) and the Crohn’s & Colitis Foundation of America. These organizations have been consistent and generous supporters of the meeting in the past. In addition, various pharmaceutical and biotechnology companies have contributed to the support of the meeting in the past, and will be enthusiastically approached again for support. 10. Advertising: Advertising for this conference will be appropriately targeted to The FASEB Journal, American Journal of Pathology, American Journal of Physiology: Cell Physiology and Gastrointestinal and Liver Physiology sections, Gastroenterology, and Journal of Clinical Investigation. We will also advertise in the official publications of the Association for Academic Surgery and Society for University Surgeons. We will employ a combination of print, online and email for advertising this conference. 11. Societies and disciplines interested in the conference: This meeting will be of interest to scientists from numerous disciplines including gastroenterology, cell and molecular biology, developmental biology, cancer biology, physiology, surgery and immunology. Members of the American Society for Investigative Pathology, American Physiological Society, American Gastroenterological Association, American Society for Cell Biology, Association for Academic Surgery, Gastrointestinal Research Group, American Federation for Medical Research, and American Cancer Society will be interested. In addition, members of the American Society of Biochemistry and Molecular Biology, and American Association of Immunologists will also have potential interest. 12. Submission: We are not planning to submit a similar application to another organization.

BIOGRAPHICAL SKETCH Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.

Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME Richard M. Peek, Jr.

POSITION TITLE Professor of Medicine and Cancer Biology Director, Division of Gastroenterology, Hepatology, and Nutrition

eRA COMMONS USER NAME (credential, e.g., agency login) EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and residency training if applicable.)

INSTITUTION AND LOCATION DEGREE (if applicable) MM/YY FIELD OF STUDY

Davidson College, Davidson, NC B.S. 1984 Pre-Medicine University of North Carolina, Chapel Hill, NC M.D. 1988 Medicine University of Alabama, Birmingham, AL Residency 1991 Internal Medicine

A. Personal Statement I developed expertise in the molecular pathogenesis of H. pylori as a research fellow in the Divisions of Gastroenterology and Infectious Diseases at Vanderbilt. As an independent investigator, our laboratory initially defined mechanisms through which H. pylori cag+ strains augment cancer risk- specifically, demonstrating that such strains dissociate epithelial proliferation from apoptosis in vivo. In mechanistic studies, our laboratory subsequently determined that adherence of H. pylori cag+ strains to gastric epithelial cells is critical for induction of inflammation and injury. Therefore, the overarching theme for our research has been delineation of the signaling events initiated by bacterial:epithelial cell contact that regulate epithelial cellular phenotypes related to carcinogenesis, particularly malignancies that arise within foci of inflammation. I have a strong background in organizing conferences and committees and overseeing large interdisciplinary programs. I organized the 2010 Gastrointestinal Response to Injury Conference sponsored by the American Gastroenterological Association (AGA), am currently Chair of the AGA Council which oversees all scientific programming for Digestive Diseases Week, and am the Principal Investigator on a large NIH-funded PPG focused on H. pylori and gastric cancer. I am also devoted to the training and mentoring of Physician-Scientists and Basic Researchers. I direct the Vanderbilt Gastroenterology Training Program (NIH T32) that consolidates Adult and Pediatric GI fellowship and postdoctoral research training at Vanderbilt, and have a vigorous role in mentoring students, residents, fellows and junior faculty. I direct the Vanderbilt Digestive Diseases Research Center, which provides significant resources to junior investigators through its rich Core and Pilot Project Program. In my role as Director of the Division of Gastroenterology, Hepatology, and Nutrition, I provide support and guidance to junior faculty and fellows in regard to career advice and advancement within academic Gastroenterology. In sum, my organizational skills, commitment to training, and laboratory expertise have me well-positioned to serve as an Organizer in this FASEB application. B. Positions and Honors Positions and Employment 1992 - 1995 Gastroenterology Fellow, Vanderbilt University Medical Center, Nashville, TN 1995 - 1996 Instructor in Medicine, Division of Gastroenterology, Vanderbilt University 1996 - 2002 Assistant Professor of Medicine, Gastroenterology, Vanderbilt University 1999 - 2004 Director of Research, Division of Gastroenterology, Vanderbilt University 2002 - 2005 Associate Professor of Medicine and Cancer Biology, Vanderbilt University 2004 - Present Director, Division of Gastroenterology, Hepatology, and Nutrition, Vanderbilt University 2005 - Present Mina Cobb Wallace Professor of Medicine and Cancer Biology, Vanderbilt University Honors 1980 - 1984 Samuel H. Bell Honor Scholarship, Davidson College 1985 - 1988 Medical School Merit Award for Academic Achievement, University of North Carolina 1987 - Present AOA Member 1988 - 1989 Osler Award - Excellence in Clinical Diagnosis by an Intern 1991 - 1992 Chief Medical Resident and Instructor in Medicine, University of Alabama at Birmingham 1994 - 1995 Grant Liddle Scholar for Excellence in Research, Vanderbilt University

1995 - 1996 Glaxo Institute of Digestive Health Basic Research Award 1995 - 1997 ADH Foundation Advanced Research Training Award 2001 - 2002 AGA Miles and Shirley Fiterman Foundation Basic Research Award 2002 - 2003 GRG/AGA Young Investigator Basic Research Award 2003 - Present American Society for Clinical Investigation 2005 - Present Mina Cobb Wallace Professor of Medicine and Cancer Biology 2006 - Present Fellow, American Gastroenterological Association 2007 - 2011 Chair, EGD Section, AGA Council 2011 - 2014 AGA Appointed Councilor, Association of Specialty Professors 2011 Vanderbilt Post-Doctoral Association Mentor of the Year 2012 - 2015 Chair, AGA Council 2013-Present Association of American Physicians C. Selected Peer-reviewed Publications (from >190) Most Relevant to the Current Application 1. Israel DA, Salama N, Arnold CN, Moss SF, Ando T, Wirth HP, Tham KT, Camorlinga M, Blaser MJ, Falkow

S, Peek RM, Jr. (2001) Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses. J Clin Invest, 107:611-20. PMCID: 199426.

2. Israel DA, Salama N, Krishna U, Rieger UM, Atherton JC, Falkow S, Peek RM, Jr. (2001) Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc Natl Acad Sci U S A, 98:14625-30. PMCID: 64732.

3. Cover TL, Krishna US, Israel DA, Peek RM, Jr. (2003) Induction of gastric epithelial cell apoptosis by Helicobacter pylori vacuolating cytotoxin. Cancer Res, 63:951-7.

4. Crawford HC, Krishna US, Israel DA, Matrisian LM, Washington MK, Peek RM, Jr. (2003) Helicobacter pylori strain-selective induction of matrix metalloproteinase-7 in vitro and within gastric mucosa. Gastroenterology, 125:1125-36.

5. Franco AT, Israel DA, Washington MK, Krishna U, Fox JG, Rogers AB, Neish AS, Collier-Hyams L, Perez-Perez GI, Hatakeyama M, Whitehead R, Gaus K, O'Brien DP, Romero-Gallo J, Peek RM, Jr. (2005) Activation of beta-catenin by carcinogenic Helicobacter pylori. Proc Natl Acad Sci U S A, 102:10646-51. PMCID: 1180811.

6. Franco AT, Johnston E, Krishna U, Yamaoka Y, Israel DA, Nagy TA, Wroblewski LE, Piazuelo MB, Correa P, Peek RM, Jr. (2008) Regulation of gastric carcinogenesis by Helicobacter pylori virulence factors. Cancer Res, 68:379-87. PMCID: 3114460.

7. Ogden SR, Wroblewski LE, Weydig C, Romero-Gallo J, O'Brien DP, Israel DA, Krishna US, Fingleton B, Reynolds AB, Wessler S, Peek RM, Jr. (2008) p120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7. Mol Biol Cell, 19:4110-21. PMCID: 2555941.

8. Franco AT, Friedman DB, Nagy TA, Romero-Gallo J, Krishna U, Kendall A, Israel DA, Tegtmeyer N, Washington MK, Peek RM, Jr. (2009) Delineation of a carcinogenic Helicobacter pylori proteome. Mol Cell Proteomics, 8:1947-58. PMCID: 2722763.

9. Nagy TA, Frey MR, Yan F, Israel DA, Polk DB, Peek RM, Jr. (2009) Helicobacter pylori regulates cellular migration and apoptosis by activation of phosphatidylinositol 3-kinase signaling. J Infect Dis, 199:641-51. PMCID: 2830010.

10. Schneider N, Krishna U, Romero-Gallo J, Israel DA, Piazuelo MB, Camargo MC, Sicinschi LA, Schneider BG, Correa P, Peek RM, Jr. (2009) Role of Helicobacter pylori CagA molecular variations in induction of host phenotypes with carcinogenic potential. J Infect Dis, 199:1218-21. PMCID: 2828761.

11. Wroblewski LE, Shen L, Ogden S, Romero-Gallo J, Lapierre LA, Israel DA, Turner JR, Peek RM, Jr. (2009) Helicobacter pylori dysregulation of gastric epithelial tight junctions by urease-mediated myosin II activation. Gastroenterology, 136:236-46. PMCID: 2678540.

12. Ogden SR, Noto JM, Allen SS, Patel DA, Romero-Gallo J, Washington MK, Fingleton B, Israel DA, Lewis ND, Wilson KT, Chaturvedi R, Zhao Z, Shyr Y, Peek RM, Jr. (2010) Matrix metalloproteinase-7 and premalignant host responses in Helicobacter pylori-infected mice. Cancer Res, 70:30-5. PMCID: 2804939.

13. Nagy TA, Wroblewski LE, Wang D, Piazuelo MB, Delgado A, Romero-Gallo J, Noto J, Israel DA, Ogden SR, Correa P, Cover TL, Peek RM, Jr. (2011) Beta-catenin and p120 mediate PPARδ-dependent proliferation induced by Helicobacter pylori in human and rodent epithelia. Gastroenterology, 141:553-64. PMCID: 3152603.

14. Noto JM, Gaddy JA, Lee JY, Piazuelo MB, Friedman DB, Colvin DC, Romero-Gallo J, Suarez G, Loh J, Slaughter JC, Tan S, Morgan DR, Wilson KT, Bravo LE, Correa P, Cover TL, Amieva MR, Peek RM, Jr. (2013) Iron deficiency accelerates Helicobacter pylori-induced carcinogenesis in rodents and humans. J Clin Invest, 123:479-92. PMCID: 3533289.

15. Noto JM, Khizanishvili T, Chaturvedi R, Piazuelo MB, Romero-Gallo J, Delgado AG, Khurana SS, Sierra JC, Krishna US, Suarez G, Powell AE, Goldenring JR, Coffey RJ, Yang VW, Correa P, Mills JC, Wilson KT, Peek RM, Jr. (2013) Helicobacter pylori promotes the expression of Kruppel-like factor 5, a mediator of carcinogenesis, in vitro and in vivo. PLoS One, 8:e54344. PMCID: 3553174.

D. Research Support Ongoing Research Support 5R01 CA077955-15 Peek (PI) 04/01/2013 - 03/31/2018 NIH/NCI H. pylori Relationship to Digestive Diseases and Cancer The focus of this grant is to investigate the role of p120 in gastric cancer that is induced by H. pylori. 5R01 DK058587-11 Peek (PI) 07/01/2011 - 06/30/2016 NIH/NIDDK Helicobacter pylori and gastrointestinal biology The focus of this grant is to define the relationship between H. pylori and Nod1 activation. 5P01 CA116087-05 Peek (PI) 01/01/2009 – 12/31/2013 NIH/NCI H. pylori-induced inflammation and gastric cancer The goal of this Program Project Grant is to define the role of aberrant epithelial signaling induced by pathogenic H. pylori strains in gastric carcinogenesis. 2P30 DK058404-11 Peek (PI) 06/01/2012 - 05/31/2017 NIH/NIDDK Molecular and Cellular Basis of Digestive Diseases The specific aims are to: 1) promote digestive diseases-related research in an integrative collaborative and multidisciplinary manner to enhance the basic research capabilities of its investigators; 2) seek to attract investigators not involved in digestive diseases-related research to pursue these lines of investigation; 3) develop and implement programs for training and establishment of young investigators in digestive diseases-related research; and 4) facilitate the transfer of basic research findings to the clinical arena. 5T32 DK007673-20 Peek (PI) 08/01/2008 - 06/30/2013 NIH/NIDDK Training in Gastroenterology The objective of the program is to prepare pre-and postdoctoral fellows for investigative careers in digestive diseases by training them in basic research utilizing molecular, genetic and cellular methods or in clinical research through formal studies in clinical science/epidemiology. 5R01 CA138833-03 Zaika (PI) 08/01/2010 - 01/31/2015 NIH/NCI p53 Family in Gastric Cancer Development This application proposes comprehensive studies focused on p53 family members which may provide new avenues for the development of novel prognostic and therapeutic targets for gastric cancer prevention. Role: Co-Investigator

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.

Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME Mills, Jason C.

POSITION TITLE

Associate Professor eRA COMMONS USER NAME JCMILLS EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE (if applicable) YEAR(s) FIELD OF STUDY

Washington University, St. Louis MO AB 1989 Biology and Russian University of Pennsylvania School of Medicine MD, PhD 1997 Cell Biology Barnes-Jewish Hospital Residency Training, St.

Louis 1997-2000 Pathology

Washington University, St. Louis, MO 2000-2003 Post-doctoral training A. Personal Statement I am a human pathologist and cell and developmental biologist. My laboratory now works on metaplasia and regulation of stem cell homeostasis in the GI tract. In particular, we are interested in how gastric epithelial cells in adults form from the stem cell and how this process goes askew during chronic Helicobacter infection. I have organized several Retreats here at Washington University and have chaired and organized several sessions in international meetings, as detailed below. I am also the organizer of a meeting in Les Treilles, France on reprogramming/dedifferentiation in the GI tract. I am director of the Washington University Digestive Disease Center Pilot and Feasibility grant award program and co-director of the entire center itself. I have peer reviewed for over 40 journals, with particular focus (40 assignments) for the premier digestive disease journal Gastroenterology. I am a handling editor for the American Physiological Society journal Physiological Genomics, so I am well abreast of the latest in GI research, including the rising stars who are currently junior, as well as all my colleagues at all stages who are doing the most innovative, fundamental work in the field. I hope to help bring all my experience to bear to help organize a memorably successful FASEB in 2015. B. Positions and Honors Positions and Employment 1989-1997 Student in University of Pennsylvania M.D., Ph.D. program 1990–1993 Research rotations in laboratories - Dr. Vivianne Nachmias, John Weisel and Joe

Sanger studying megakaryocyte microtubules and cell-to-cell spread of Listeria monocytogenes

1993-1996 Ph.D. research, laboratory of Dr. Randall Pittman 1997-1999 Resident, Anatomical Pathology, Barnes-Jewish Hospital 1999-2002 Clinical Fellow, Washington University School of Medicine, St. Louis, MO 1999-2003 Research Associate, Molecular Biology and Pharmacology, Washington University School of

Medicine, Mentor: Dr. Jeffrey Gordon 2003-2004 Instructor, Molecular Biology and Pharmacology, Washington University School of Medicine, St.

Louis, MO, Mentor: Dr. Jeffrey Gordon 2004-2010 Assistant Professor, Pathology and Immunology, Developmental Biology, Washington

University School of Medicine 2011-present Associate Professor with tenure, Medicine, Pathology and Immunology, Developmental Biology,

Washington University School of Medicine 2013-present Co-Director Washington University Digestive Diseases Research Core Center (DDRCC)

Honors and Awards (last four years) 2013 Inducted as a Fellow of the American Gastroenterological Association (AGAF) 2013 Appointed Councilor, American Gastroenterological Association, Regulatory Peptides, Cell Signaling &

Molecular Biology Section 2012 Inducted into American Society for Clinical Investigation 2011 Promoted to Associate Professor with tenure in three departments 2010 Named an Associate Editor for the American Physiological Society journal Physiological Genomics

2010 Graduate Student Senate and Graduate School of Arts and Sciences Award for excellence in graduate student mentoring

2010 Named the 2010 AGA Foundation Funderburg Research Scholar in Gastric Biology Related to Cancer 2009 Named a “Top Reviewer” by the journal Gastroenterology Meeting Organizational Experience Conference organization: 2013: 1) Midwest Society for Developmental Biology, Session Chair and speaker, St. Louis, MO; 2) FASEB Summer Conference "Gastrointestinal Tract: XV", Steamboat Springs, CO; 3) Singapore Gastric Cancer Consortium 6th Annual Meeting, SINGAPORE; 4 & 5) 2 separate symposia, American Gastroenterology Association Digestive Disease Week (AGA DDW), Orlando, FL Earlier: AGA DDW, San Diego, CA (2012); AGA DDW, Chicago, IL (2011); Washington University Regenerative Medicine Faculty Retreat, St. Louis, MO (2011); AGA DDW, New Orleans, LA (2010); Organizer Washington University Immunology Graduate Program Retreat, Pitosi, MO (2007-2009), AGA DDW (2007), Washington, D.C.; AGA DDW, Los Angeles, CA (2006) Panel/Chairman/Session Leader: 2013: 1) Midwest Society for Developmental Biology, Session Chair and speaker, St. Louis, MO; 2) FASEB

Summer Conference "Gastrointestinal Tract: XV", Steamboat Springs, CO; 3) Singapore Gastric Cancer Consortium 6th Annual Meeting, SINGAPORE; 4 & 5) 2 separate symposia, American Gastroenterology Association Digestive Disease Week (AGA DDW), Orlando, FL

Earlier: AGA DDW, San Diego, CA (2012); AGA DDW, Chicago, IL (2011); AGA DDW, New Orleans, LA (2010); AGA DDW (2007), Washington, D.C.; AGA DDW, Los Angeles, CA (2006)

C. Publications (from over 65 articles and chapters already published or in press)

1. Ramsey VG, Doherty JM, Chen CC, Stappenbeck TS, Konieczny SF, Mills JC: The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Development 2007;134:211-222. (This article featured by editors in “In This Issue”). PMID: 17164426

2. Cella M, Fuchs A, Vermi W, Facchetti F, Otero K, Lennerz JK, Doherty JM, Mills JC, Colonna M: A human natural killer cell subset provides an innate source of IL-22 for mucosal immunity. Nature 2009;457:722-725. PMID: 18978771

3. Bredemeyer AJ, Geahlen JH, Weis VG, Huh WJ, Zinselmeyer BH, Srivatsan S, Miller MJ, Shaw AS, Mills JC: The gastric epithelial progenitor cell niche and differentiation of the zymogenic (chief) cell lineage. Dev Biol 2009;325:211-224. PMCID: PMC2634829

4. Huh WJ, Mysorekar IU, Mills JC: Inducible expression of Cre recombinase in adult mice causes gastric epithelial atrophy, metaplasia and regenerative changes in the absence of "floxed" alleles. Am J Physiol Gastrointest Liver Physiol 2010:299:G368-380. PMID: 20413717. PMC Journal – In Process

5. Tian X, Jin RU, Bredemeyer AJ, Oates EJ, Blazeswka KM, McKenna CE, Mills JC: RAB26 and RAB3D are direct transcriptional targets of MIST1 that regulate exocrine granule maturation. Mol Cell Biol 2010;30:1269-1284. PMC2820885

6. Huh WJ, Esen E, Geahlen JH, Bredemeyer AJ, Lee AH, Shi G, Konieczny SF, Glimcher LH, Mills JC. XBP1 controls maturation of gastric zymogenic cells by induction of MIST1 and expansion of the rough endoplasmic reticulum. Gastroenterology 2010;139:2038-2049. PMC2997137

7. Nam KT, Lee HJ, Sousa JF, O’Neal RL, Finke PE, Romero-Gallo J, Shi G, Mills JC, Peek RM Jr, Konieczny SF, Goldenring JR. Mature chief cells are cryptic progenitors for metaplasia in the stomach. Gastroenterology 2010;139:2028-2037. PMC2997152

8. Lennerz JKM, Kim S-K, Oates EJ, Tian X, Bredemeyer AJ, Goldenring JR, Lauwers GY, Shin Y-K, Mills JC: The transcription factor MIST1 is a novel human gastric chief cell marker whose expression is lost in metaplasia, dysplasia and carcinoma. Amer J Pathol 2010;177:1514-1533. (Chosen for Cover Illustration) PMC2928982

9. Cappocia BJ, Lennerz JKM, Bredemeyer AJ, Klco JM, Frater JL, Mills JC. The transcription factor MIST1 in terminal differentiation of mouse and human plasma cells. Physiol Genomics 2010;43:174-186. PMC3055710

10. Goldenring JR, Nam KT, Mills JC: The origin of pre-neoplastic metaplasia in the stomach: Chief cells emerge from the Mist. Exp Cell Res 2011;317:2759-2764. PMC3210373

11. Mills JC, Taghert PH: Scaling Factors: transcription factors regulating subcellular domains. Bioessays 2012;34:10-16.

12. Huh WJ, Khurana SS, Geahlen JH, Kohli K, Waller RA, Mills JC. Tamoxifen induces rapid, reversible atrophy and metaplasia in mouse stomach. Gastroenterology 2012; 142:21-24. PMID: 22001866

13. Capoccia BJ, Jin RU, Kong Y-Y, Peek RM Jr, Fassan M, Rugge M, Mills JC. The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation. J Clin Invest 2013;123:1475-1491. PMC3613919

14. Khurana SS, Riehl TE, Moore BD, Fassan M, Rugge M, Romero-Gallo J, Noto J, Peek RM, Stenson WF, Mills JC: The hyaluronic acid receptor CD44 coordinates normal and metaplastic epithelial progenitor cell proliferation. J Biol Chem In Press (Selected for Cover Illustration, May 31, 2013).

15. Stange DE, Koo B-K, Huch M, Sibbel G, Basak O, Lyubimova A, Kujala P, Bartfeld S, Koster J, Geahlen JH, Peters PJ, van Es JH, van de Wetering M, Mills JC, Clevers H: Differentiated Troy+ chief cells act as “reserve” stem cells to generate all lineages of the stomach epithelium. Cell; In Press.

D. Research Support Ongoing Research Support ACTIVE

1 R01 DK094989-01 (PI: Mills, award $198,673) 9/11/2012-6/30/2017 NIH/NIDDK Regulation of atrophy-induced progenitor cells in the gastric corpus The goal of this study is to determine the molecular mechanisms underlying regulation of proliferation of gastric corpus epithelial stem cell, focusing especially on its response to atrophy (death) of parietal cells in models of atrophic gastritis/pseudopyloric metaplasia. Atrophy-induced progenitors will be cultured, and pathways involved in their proliferation determined (eg, ERK, CD44, STAT3).

5 R01 NS021749-25 (PI:Taghert, subaward $9,4896/yr) 01/01/2009-12/31/2013 NIH/NINDS Developmental Regulation of Neuropeptide Expression The major goal of this project is to study a Drosophila model of neuronal differentiation. We examine the mechanisms by which neuroendocrine phenotypes are established, and how the Mist1 related bHLH protein called DIMM organizes a common program of regulated neurosecretion in different peptidergic neurons. Role: Co-PI

RSG-12-171-01-LIB (PI: Di Paolo – SLU, subaward $23,576/yr) 07/01/2012-06/30/2016 ACS The Regulation of Gastric Cancer in a Model of Autoimmune Gastritis The objectives of this proposal are to generate a detailed analysis of the gastric carcinogenesis process in

this new mouse model and to determine how this process is influenced by regulatory T cells. We anticipate that adoptive transfer of regulatory T cells will provide an effective therapeutic intervention by suppressing inflammation and interrupting the process leading to gastric carcinogenesis. The experiments outlined in this proposal are unique in that the pre-cancerous lesions (SPEM) develop as a result of autoimmunity and they are the first to examine the potential of antigen specific Tregs to regulate steps in gastric carcinogenesis. Role: Co-Investigator (PI at WU site)

5 R01 DK079798-05 (Mills) 03/01/2008 – 02/28/2013(NCE-2014) NIH/NIDDK Molecular regulation of gastric chief (zymogenic) cell differentiation The major goals of this project is on the role of Mist1 in mediating structural changes during chief cell terminal differentiation, focusing on Mist1 interactions with Cd2ap, on the phenotype of Cd2ap/Mist1 double knockout mice, on cell morphology changes caused by MIST1 transfection, and on targets Mist1 may regulate in vivo in mice.

Role: PI

Program Director/Principal Investigator (Last, First, Middle): Wong, Melissa Hirose

PHS 398/2590 (Rev. 06/09) Page Biographical Sketch Format Page

BIOGRAPHICAL SKETCH Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.

Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME

Melissa Hirose Wong POSITION TITLE

Associate Professor; Vice Chair, Cell and Developmental Biology; Program Leader, Knight Cancer Institute

eRA COMMONS USER NAME (credential, e.g., agency login)

wongme

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and residency training if applicable.)

INSTITUTION AND LOCATION DEGREE

(if applicable) MM/YY FIELD OF STUDY

University of Colorado, Boulder, CO B.A. 1987 Molecular, Cellular, Developmental Biology

Bowman Gray School of Medicine/Wake Forest University, Winston-Salem, NC

Ph.D. 1994 Molecular Cellular Pathobiology

Washington University School of Medicine Post-Doc 1995-1998 Molecular Biology and Pharmacology

A. Personal Statement The research focus of the Wong laboratory revolves around understanding the regulatory mechanisms that

control epithelial stem cell homeostasis and expansion in developmental and disease contexts, including inflammatory bowel disease, colorectal cancer, pancreatic cancer and head and neck squamous cell carcinoma. We have particular interest in mechanisms that regulate the metastatic phase of cancer. Our laboratory has identified cell fusion between macrophages (MФs) and cancer cells as one potential mechanism by which cancer cells can gain properties of migration, immune surveillance, extravasation, and colonization at a distant site. Intriguingly, all of these functional abilities are shared by both MФs and metastatic cancer cells, providing the basis for investigating how cell fusion between these two cell types represents one mechanism by which cancer cells can be reprogrammed to exhibit metastatic behavior. We have previously demonstrated that the MФ-cancer fusion hybrid undergoes aberrant reprogramming in the context of cancer by transcriptome analyses. In addition, my laboratory is actively engaged in studies to understand the regulatory niche that controls stem cell hierarchy. My group is a member of the NIDDK/NIAID funded Intestinal Stem Cell Consortium.

I have organized the 2012 Intestinal Stem Cell Consortium meeting sponsored by the NIDDK, am currently Vice Chair of the department of Cell and Developmental Biology and Program Leader of the Cancer Biology program in the Knight Cancer Institute. In addition, I am an active mentor (and co-director) of the Mucosal Pathobiology Training Program (NIH T32). Further, I am actively involved in mentoring junior faculty in my department and in the department of Surgery. Overall, my leadership skills demonstrate my commitment to GI research, mentorship and career development of junior scientists and to successfully running my laboratory group. B. Positions and Honors Positions and Employment 1998-2001 Instructor, Molecular Biology and Pharmacology, Washington University School of

Medicine 2001-2008 Assistant Professor, Dept of Dermatology and Cell & Developmental Biology, School of

Medicine, Oregon Health & Science University, Portland, OR 2008-present Associate Professor, Dept of Cell & Developmental Biology and Dermatology, School of

Medicine, Oregon Health & Science University, Portland, OR 2013-present Vice Chair, Department of Cell & Developmental Biology, Oregon Health & Science

University, Portland, OR Honors and Other Professional Activities: 1989-1990 Dean’s Fellowship 1992-1995 National Research Service Predoctoral Award, NIDDK

Program Director/Principal Investigator (Last, First, Middle): Wong, Melissa Hirose

PHS 398/2590 (Rev. 06/09) Page 2 Continuation Format Page

2001-present Knight Cancer Institute, OHSU, member/program leader 2003-present Developmental Biology Society 2003-present American Gastroenterology Association 2003-present International Society for Stem Cell Research 2004-present Oregon Stem Cell Institute, adjunct member 2006-2007 Ad hoc reviewer: NIH/NCI SEP ACA1 RTRB-A 2007-2013 NIH/NCI MONC 2008 Reviewer: NIH/NCI P01 Cancer Stem Cells 2009-present NIH/NIDDK Intestinal Stem Cell Consortium Steering Committee C. Selected Peer-reviewed Publications *Hermiston ML, *Wong, MH, and JI Gordon. (1995) Forced expression of E-cadherin in the mouse intestinal

epithelium slows cell migration and provides evidence for nonautonomous regulation of cell fate in a self-renewing system. Genes Dev. 10:985-996. (*equal contribution)

Wong MH, Rubinfeld, B and Gordon JI (1998) Effects of Forced Expression of an NH2-terminal Truncated -Catenin on Mouse Intestinal Epithelial Homeostasis. J. Cell Biol. 141:765-777.

Wong MH, Saam JR, Stappenbeck TS, Rexer CH, and Gordon JI. (2000) Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection. Proc. Natl. Acad. Sci. USA, 97: 12601-12606.

Hooper, LV, Wong, MH, Thelin, A, Falk, PG, and Gordon, JI. (2001) Molecular analysis of commensal host-microbial relationships in the intestine. Science, 291:881-884.

Wong, MH, Huelsken, J, Birchmeier, W, and Gordon JI. (2002) Selection of multipotent stem cells during morphogenesis of small intestinal crypts of Lieberkuhn is perturbed by stimulation of Lef-1/beta-catenin signaling. J. Biol. Chem. 277:15843-15850.

Bailey, AS, Jiang, S, Afentoulis, M, Baumann, CI, Schroeder, DA, Olson, SB, Wong, MH, and Fleming, WH. (2004) Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells. Blood. 103:13-19.

Rizvi AZ, Swain JS, Bailey AS, Davies PS, Decker AD, Willenbring H, Grompe M, Fleming WH, and Wong MH. (2006) Bone marrow-derived cells fuse with normal and transformed intestinal progenitor cells, Proc. Natl. Acad. Sci. USA,103:6321-5. [PMCID: PMC1535365]

Bailey AS, Willenbring, H, Jiang S, Anderson DA, Schroeder DA, Wong MH, Grompe M and Fleming WH. Myeloid lineage progenitors give rise to vascular endothelial cells. (2006) Proc. Natl. Acad. Sci. USA, 103:13156-61. [PMCID: PMC1559769]

Davies PS, Dismuke, AD, Powell AE, Carroll KH, and Wong MH. Wnt signaling expression pattern in the mouse intestine during homeostasis. (2008) BMC Gastroenterol, 8:57. [PMCID: PMC journal in process]

Davies PS, Powell AE, and Wong MH. (2009) Inflammation and proliferation act together to mediate intestinal cell fusion. PLOS One, 4:e6530. [PMCID: PMC2716548]

Levin TG, Powell AE, Davies PS, Silk AD, Dismuke AD, Anderson EC, Swain JR, and Wong MH. (2010) Characterization of the intestinal cancer stem cell marker CD166 in the human and mouse gastrointestinal tract. Gastroenterology, 139:2072-2082. [PMCID: PMC2997177]

Powell AE, Davies PS, Anderson EA, Silk AD, Pelz C, Impey S and Wong MH. (2011) Fusion between Intestinal epithelial cells and Macrophages in a cancer context results in nuclear reprogramming. Cancer Research, 71:1497-1505. [PMCID: PMC3079548]

Clayburgh DR, Gross ND, Proby C, Koide J, and Wong MH. (2013) Resistance to Epidermal Growth Factor Receptor Inhibition in Cutaneous Squamous Cell Carcinoma of the Head and Neck through upregulation of insulin-like growth factor 1 receptor signaling: Potential for dual inhibition as a therapeutic modality. Head Neck. 35:86-93. [PMCID:PMC3605039]

Wang F, Scoville D, Box A, Haug JS, von Furstenberg R, Gracz A, Henning S, Magness S, Wong M, Yu J, Ding S, He XC, and Li L. (2013) Characterizing intestinal stem cells using robust clonal assay and surface markers. Gastroenterology. 145:383-95. [PMCID:PMC in progress]

Silk AD, Gast CE, Davies PS, Fakhari FD, Vanderbeek GE, Mori M, Wong MH. (2013) Fusion between hematopoietic and epithelial cells in adult human intestine. PLoS One. 8:e55572. [PMCID: PMC3559593]

Program Director/Principal Investigator (Last, First, Middle): Wong, Melissa Hirose

PHS 398/2590 (Rev. 06/09) Page 3 Continuation Format Page

D. Research Support Active Grant Support

NIH/NIDDK U01DK085525 (Wong) 9/1/09-8/31/14 Characterization of intestinal stem cells Role: PI

NIH/NCI CA172334 (Wong) 9/1/13-8/31/15 Novel mechanisms of cancer metastasis Role: PI NIH/GM R01 GM32741-28 (Liskay) 9/1/10-8/30/14 Effects of Stochastic gene alterations on intestinal tumorigenesis in the mouse Role: Co-Investigator NIH/NCI CA151564 (Thomas) 3/4/11-2/19/16 Regulation of Trail induced apoptosis in cancer cells Role: Co-Investigator NIH/NIDDK U01DK085525-04S1 (Wong) 9/1/12-8/31/14 Hierarchical relationships of intestinal stem and progenitor cells during development Engineering the small intestine using an acellular scaffold Role: PI NIH/NIDDK U01DK085525-05S1 (Wong) 9/1/13-8/31/14 Crypt/villus-associated intestinal macrophages in homeostasis and response to injury Reprogramming Human ISCs to Produce Insulin Secreting cells FOX01 Inhibition Role: PI Women’s Health Research Grant (Wong) 2/1/13-1/31/14 Novel mechanisms for metastatic spread of breast cancer Role: PI Crohn’s & Colitis Foundation of America (Wong) 1/1/13-12/31/15 Macrophage-epithelial fusion in Crohn’s disease Role: PI Grants for Cancer Research (Wong) 2/1/13-1/31/14 Anna Fuller Fund Cell fusion: A novel mechanism for metastatic cancer Role: PI

Completed Grant Support

NIH/NCI 1R01CA118235 (Wong) 07/06-06/12 Transplanted stem cells and tumor progression The major goals of this project are to determine how transplanted bone marrow-derived cells contribute to intestinal tumorigenesis. NIH/NIDDK 1R01DK068326 (Wong) 4/1/05-3/31/11 β-catenin’s role in establishing the gut stem cell niche The major goals of this project are to identity genes that are altered early in response to Wnt signaling for potential diagnostic approaches for pre-polyp identification of modified epithelium.

Dean’s Fund (Wong) 5/1/08-12/30/11

Course Evaluation 2013 Science Research Conferences

Gastrointestinal Tract XV: Epithelia Microbes Inflammationand Cancerpresented 8/11/2013

153 forms submitted

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Forms

Section 1 - Scientific Content

General Sessions

The most important areas of current active research were adequately discussed. 4.6

There was a sufficient amount of unpublished research presented. 4.5

The conference helped you generate new ideas for research. 4.5

There was adequate time provided for invited presentations and short talks selected from submitted abstracts. 4.7

The discussion periods were utilized effectively. 4.6

Poster Sessions

The time allocated for poster sessions was effective. 4.3

I am satisfied with the contribution of the poster sessions to the conference. 4.5

Scientific Content - Overall

Overall, I am satisfied with the scientific content. 4.7

What kinds of sessions would you like to see included at future conferences?

gut motility

All sessions were adequately represented

More Developmental Presentations.

Please add back a session on epithelial transport. Even the session labeled as transport had only tight junction talks.

More intestinal stem cell content

metabolic and obesity related GI pathobiology

Poster sessions were great, just not enough time to see so many great posters

A little more translational information, with some studies in humans or on therapies

epithelial repair, stem cell regeneration/recruitment, microbiome, systems biology of GI function/disease

Please continue doing poster session happy hours! I think the poster sessions were well attended as a result and generated great discussions.

Novel imaging techniques

Cutting edge technology in the field

Less emphasis on mucosal immunology and more on epithelial cell biology and physiology.

inflammation associated cancer and genomic instability

I think the poster sessions could be split over three days, if there are enough posters. Also, one of the sessions was only 90 minutues long- I really think 2 hours isideal.

I would like to have more career development activities for trainees, as well as more information on epithelial/stem cell biology. Too many sessions (particularly theimmunology sessions) were too focused and did not appeal to the broader audience.

More small group sessions might allow for easier discussions.

more stem cells

hormonal/ENS control of gi physiology/disease

Gastrointestinal Tract XV: Epithelia Microbes Inflammation and Cancer http://www.planion.com/Planion.Evals/ZZHWPBUU4Z?ACCOUNT=...

1 of 5 10/25/2013 4:04 PM

transduction

the mix was perfect! Congratulations.

more development

I really enjoyed the talks on the second day of the conference and would be interested to see more science along this vein at future meetings (i.e., function of themicrobiome, interactions of microbiome and disease). It would be interesting to have a session centered around the latest IBD research. I thought that overall there wasa good mix of topics at this meeting.

Perhaps a session dedicated to translational gut biology.

Metabolism, LKB1

Polarity and cancer

More gut physiology including roles of secretory and absorptive cells as well as hormonal controls, more stem cells, intestinal tissue engineering

I was a bit disappointed with the developmental biology session.

Section 2 - Management

Program Management & Organization

How satisfied were you with the coordination and organization of the scientific program? 4.6

How satisfied were you the representation of international scientists in this field participating? 4.4

How satisfied were you with the conference materials provided? 4.4

Did you feel the length of conference sessions were too long, just about right, or too short? 2.4

(3=Too long; 2= Just about right; 1=Too short)

Logistics Management & Organization

How satisfied were you with the registration and abstract submission process? 4.5

How satisfied were you with the information found on the FASEB SRC website and from emails sent by the FASEB SRC Office? 4.3

Overall Management & Organization

The conference was well organized. 4.6

Conference onsite staff member was helpful and courteous. 4.7

Overall, I was satisfied with the conference facilities. 4.5

Where would you like to see future SRCs take place?

Steamboat CO

Steamboat is a nice venue. Would like to learn more about other sites such as Montana.

Steamboat Springs seems like a perfect venue

yes

Steamboau Springs

Steamboat Springs, Santa Fe

Steamboat is fine

Smoky mountains

cool area if mid-summer: colorado perfect for east and west coast access

Loved Steamboat Springs and the Steamboat Grand! Would return in a heartbeat.

Steamboat was great

steamboat springs

Steamboat again

I liked steamboat springs because the conference, food, etc. was all in one place- we didn't have to drive a ton to get to where we needed to be. I also liked beingoutside the city- many of us work in large cities, and it is so nice to discuss science in this different environment.

I think the Steamboat location is best.

Steamboat is perfect for this conference. The group is large and the Steamboat facility is big enough to accomodate it.

I liked this venue

canada

Santa Fe, NM

Gastrointestinal Tract XV: Epithelia Microbes Inflammation and Cancer http://www.planion.com/Planion.Evals/ZZHWPBUU4Z?ACCOUNT=...

2 of 5 10/25/2013 4:04 PM

All was great but the food. lacking seriously of healthy choices

steamboat spring

Steamboat

Steamboat Springs

Montana

I love attending the meeting at Steamboat, CO.

Cancun, Puerto-rico

Montana!

Colorado works well or anywhere else central. California, although not central would also be nice.

Steamboat Springs

Steamboat is a very convenient location.

same venue

Big Mountain, Montana

Snowmass, Whitefish, Big Sky

Long Island, New York or some place with convenient public transportation

I would really like to see the meetings take place at any location that is more convenient and is a more economic choice. It was very expensive to fly to Steamboat. Theoption of flying to Denver and then driving was also expensive. Moreover, flights into and out of Steamboat were quite limited.

Which months are more convenient for you to attend a conference?

June - August: 114 (87%)

September - November: 8 (6.1%)

March - May: 7 (5.3%)

December - February: 2 (1.5%)

Overall, how would you rate the FASEB SRC Staffs' professionalism and responsiveness to your questions and concerns? 4.7

Comments:

The staff person was new and the senior person apparently did not show up. The organizers were not given information re: other sites for future meetings or potentialdates for our meeting. This made the business meeting ineffective for addressing all of the issues for the next meeting!!

The food at the hotel was very mediocre this year. Are there options for other venues in Steamboat?

please send PDF file with the conference material before conference starts!

The staff member present was courteous but overwhelmed, as she was clearly inexperienced.

There was a bit of confusion with the scheduling of talks one of the days. The double booking of time slots was confusing and as a result was a very long night. Manypeople left early which isn't fair to the later presenters.

Nothing in particular but if FASEB can arrange a shuttle from Denver airport to the conference site, will be very helpful. As a lot of time was wasted in waiting ormatching the only shuttle Go Alpine schedule. If FASEB provides the shuttle with more frequent timings on first and last day will save a lot of time of attendees.

The hotel information came very late.

There was great balance between younger investigators and established investigators and between men and women speakers at this meeting. The organizers did agreat job selecting speakers.

the off site activity was not well organized. conference in places such steamboat should include a well organized entertainment of -site activity

Overall, the management issues that stuck out was the lack of information on poster/presentations in the weeks before the conference. I realize a lot goes in toplanning, but it would be nice to have this information a little farther in advance. Also, I would have liked some sort of order to the printed material and abstracts to beable to find information more quickly.

The food was pretty poor for what we paid and the vegetarian or non-meat (fish) options were really poor throughout.

I understand that evaporating funds make it impossible to guarantee reimbursement for invited speakers but a room and registration should be set aside for speakersthat have agreed to attend. Because I was grantwriting and have no administrative assistant I registered one day late and had to pay both for a room wiht a guest,which I didn't want and a big late fee - I emailed FASEB asking about this and received no response at all. I will ultimately pay more than $3,000 out of pocket to attenda meeting that was really great but one I would not have normally attended.

Section 3 - General Information

Approximately how many conferences of this type do you attend annually?

1-2 per year: 120 (80.5%)

3-4 per year: 23 (15.4%)

5-6 per year: 2 (1.3%)

More than 6 per year: 0 (0%)

Don't usually attend conferences: 4 (2.7%)

Do you plan to attend this conference again in 2 or 3 years?

Yes: 136 (91.9%)

No: 12 (8.1%)

Would you recommend this conference to others? How would you rate this conference compared to other conferencesof this type that you have attended? 4.6

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Yes: 149 (97.4%)

No: 0 (0%)

Not Sure: 1 (0.7%)

No response: 3 (2%)

How did you learn of this conference?

Co-Worker: 70 (46.1%)

By Invitation: 45 (29.6%)

Internet: 13 (8.6%)

Other: 9 (5.9%)

FASEB Emails: 8 (5.3%)

FASEB Mailings: 6 (3.9%)

ExperimentalBiology/Neuroscience/Cell

Biology:1 (0.7%)

FASEB Journal: 0 (0%)

If other, please specify:

previouslyattended:

1 (9.1%)my former

mentor:1 (9.1%)

I am aregular:

1 (9.1%)have

attendedbefore:

1 (9.1%)

previousattendee:

1 (9.1%)

I havebeen

attendingthis

conferencesince the1st one

and:

1 (9.1%)

help toorganizein earlier

years:

1 (9.1%) Colleague: 1 (9.1%)

previousattendance:

1 (9.1%)

I haveattended

thisconference

for yearsso am

aware ofit.:

1 (9.1%)

Have beenattending

thisconference

for over10 years:

1 (9.1%)

Please indicate your age group:

20's: 33 (21.7%)

30's: 49 (32.2%)

40's: 24 (15.8%)

50's: 32 (21.1%)

60's: 13 (8.6%)

70's: 1 (0.7%)

In what ways could this conference be improved?

There were 2 typos in the program that altered the times causing the sessions to run too long. Program should not go beyond 9 at night. Moderators need to be morestrict on limiting presentation times and discussion so that the program runs on time.

abstracts in conference booklet were unsorted, so it was very timeconsuming to identify the abstracts of interest and pair them with the session they were presented

The SRC I attended 2 years ago had more special lectures (an animal models lecture, and a talk from the NIH about grant funding), this conference didn't have anythinglike that. Also, the talks seemed to be very narrowly focused on several of the days, with several talks almost overlapping. I would have been nice to see more breadthof research rather than so much focus on narrow areas of mucosal immunology or epithelial barrier proteins.

Longer poster session than just 1.5hours.

In all area the conference was well organized and the scientific presentation was informative. This is in agreement with my colleagues

The program had quite a few errors. It would have been helpful to have the program organized having the abstract number next to the scientists names/addresses/email at the end.

Making sure that speakers stay on time. Some chairs made little or no effort to cut people off. Some speakers had dozens of slides for a 10 or 20 minute talk, leavinginadequate time for discussion.

keep doing what you were doing

Evening sessions could be shorter since they typically go over time

make sure evening sessions end at 9pm. having meeting as early in august as possible to avoid classroom conflicts.

The inclusion of meals and coffee breaks was wonderful, but there was no diet coke! Definitely need some refreshing caffeine in the afternoons.

I think that maybe if the sessions were intermixed in content then they would be more enjoyable. Plus, the speakers would have a more diverse crowd.

I thought the conference was a little too long. 3 full days would be more manageable for me.

Enjoyed the couple of presentations by younger faculty (Assistant Prof etc). I would be useful to keep this going or even slightly expand to mix well w/ the highlyestablished investigators.

many details on the printed program should be fixed - timings of talks and things like a table of contents to link talks and abstracts to attendees

Better poster sessions.

Better balance, with less on mucosal immunology and more on epithelial cell regulation.

It was just perfect, please try to accommodate a shuttle exclusive for attendees at least on first and last day of the conference, will be very convenient. I landed at 9.25am at the Denver airport and reached to the conference site at 5.30 pm. (a huge amount of time was wasted)

Having more activities for trainees (career development sessions, interactions with NIH staff, etc) would be helpful. Also, I think having more short talks by traineeswould be better for presenting unpublished data.

extend the poster sessions.

It would be nice if for at least a couple days the sessions ended before dinner so that we could have a free evening.

more time for open discussion at poster session

optimal as is

It was difficult to find specific abstracts in the book. They may be presented in alphabetical order or the abstract number may be added to the participants' list and/or tothe program.

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The itinerary had several typos and wrong times. It would be nice to have the poster number for each person next to their name in the index so that they can easilyfind their number instead of having to flip through every abstract.

The lecture room was over air conditioned.

the meals at the conference hotel were not super great. could be improved.

expand propaganda and invite more excellent scientists come from different countries to attend the conference

It really was terrific as it was. It was great having free afternoon time and evening sessions. Very collaborative environment.

A little more diversity in topics for short talks, also conference food was terrible for vegetarians. Hotel food (paid for individually) was outstanding so site can dobetter...

Better food.Do not go past 9 PM for night sessions.

More time for posters and more spacious room for them. Abstracts in booklet should be listed alphabetically by first author.

see above

Although the conference facilities are fine, I found it difficult to find economic flights at convenient times to get to the meeting. It would have actually been cheaper forme to go to the Bahamas vs. Colorado for this meeting! The vegetarian offerings for food should also be better. There was one lunch in which I could find nothingsatisfactory to eat. There needs to be more time allotted to posters, and there needs to be more space between the posters. It was very difficult to navigate the aislesduring the poster session. It would have been helpful to organize the poster abstracts according to discipline or at least according to the session in which the posterswould be presented. The program needed some proofreading too. There were a lot of mistakes in it.

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FINAL REPORT

FASEB SUMMER CONFERENCE Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer

August 11-15, 2013 Steamboat Springs, Colorado

Organizers:

Asma Nusrat Robert Coffey

Thaddeus Stappenbeck

The 2013 “Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer” meeting was recently held in Steamboat Springs, Colorado, from August 11-15, 2013. This was the 15th in a series of conferences focused on the gastrointestinal tract that has been held biannually since 1985. The attendees of the 2011 meeting voted in unanimous agreement that another conference in this area was needed, and we are happy to report that this year’s meeting was both well-attended and scientifically well-received. Research pertaining to biology and pathophysiology of the gastrointestinal tract is progressing at a very rapid pace, and the community of scientists working in this area view this conference as an ideal venue for updates on the most current scientific advancements. This meeting is further necessary because it combines research endeavors impacting not only gastrointestinal pathophysiology, but also general aspects of stem cell and epithelial biology, mucosal inflammation and immune response, impact of microbiota on gut pathobiology, and cancer pathogenesis. Indeed, investigators working in the gastrointestinal sciences widely regard this meeting as the premier scientific conference in the field. For the 2013 meeting, expert speakers were invited to present research developments in new topic areas covering both technological and conceptual advances that have previously not been presented. Internationally renowned scientists from North America and abroad presented their work and chaired the sessions, which were attended by many trainees, as well as junior and senior scientists. In total, there were approximately 170 attendees, including 35 invited speakers and organizers. The meeting began on Sunday evening with three keynote presentations on recent advances in mouse modeling of GI disorders, given by Drs. Nancy Jenkins and Neal Copeland from the Methodist Hospital Research Institute, and by Dr. David Threadgill from North Carolina State University. These speakers presented cutting-edge developments in harnessing transposons for cancer gene discovery in the GI tract, and modeling population heterogeneity to understand individual cancer susceptibility. The following four days were organized around daily themes, including Developmental Biology and Cancer Stem Cells (Monday), Microbial Ecology/Commensal Bacteria and Host-Pathogen Interactions (Tuesday), Mucosal Immunity and Inflammation (Wednesday), and Epithelial Properties, Intercellular Junctions and Repair (Thursday). Scientific presentations included thirty-minute talks (including a discussion period) by thirty-two invited speakers, as well as nineteen shorter, fifteen-minute talks from trainees and junior investigators selected from submitted abstracts. In addition, we hosted a Career Development Workshop entitled "Meet the Experts," that was led by senior investigators Drs. Kim Barrett, Gail Hecht, and Marshall Montrose. In all, ten of the invited speakers and

three of the eight session chairs were women. Seventy-eight percent of the invited speakers had not presented at the previous two meetings. We also hosted two, two-hour catered poster sessions (on Monday and Tuesday) at which 91 investigators were able to present and discuss their research, including an impressive amount of unpublished data. Six accomplished investigators served as judges for the poster sessions, and a total of six poster awards were given. All attendees received a bound volume containing submitted abstracts, thereby facilitating cross-pollination of scientific ideas. The intimate and informal setting of the meeting allowed for high-quality interactions, as evidenced by many fruitful discussions during oral and poster sessions. Graduate students and postdoctoral fellows commented that the format of this conference was highly conducive to productive learning, scientific exchange of information, networking, and career advancement. Attendees also indicated that this meeting in particular allowed them to establish new collaborations while interacting with experts in the field. Funding to support this meeting was obtained from a number of sources, including FASEB Summer Research Conferences, NIH/NIDDK, the Crohn’s and Colitis Foundation of America, Pfizer, MultiOmyx-GE Healthcare, the Jackson Laboratory, Vertex, and Genentech. Funds were used toward registration costs for speakers and chairs, as well as the six poster award winners and seventeen trainees and junior faculty whose abstracts were selected for short oral presentations. A business meeting was held on the final day of the conference, at which Drs. Melissa Wong, Richard M. Peek, and Jason Mills were selected by attendees as organizers of the next meeting. The group unanimously agreed that the field is growing rapidly and that there is a strong need to hold this meeting again in 2015, and went on to vote for Steamboat Springs, Colorado as the top choice of location (based on a post-meeting poll, the second and third choices were Nassau, Bahamas and Big Sky, MT, respectively). In summary, the organizers of this FASEB Summer Conference feel that this meeting was a tremendous success, and we look forward to another exciting conference in 2015. Sincerely, Asma Nusrat Organizer, Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer.

Year Name Affiliation Year Name Affiliation1985 Leonard Lichtenberger Univ. of Texas Med. Sch. 2005 Marshall Montrose Indiana Univ.

Susan Henning Univ. of Houston Vincent Yang Emory Univ.Jerrold Turner The Univ. of Chicago

1987 Leonard Johnson Univ. of Texas HSCJames Jamieson Yale Univ. Sch. Of Med. 2007 D. Brent Polk Vanderbilt Univ. Medical Center

Juanita Merchant Univ. of Michigan Med. School1989 Jackie Wood Ohio State Univ. Beth McCormick Mass General Hospital / Harvard Med. Sch.

Gilbert Castro Univ. of Texas HSC2009 Shanthi V. Sitaraman Emory Univ.

1991 Mike GershonColumbia Univ. of Physicians and Surgeons Wayne Lencer Harvard Medical School

Marion Neutra Harvard Univ. Cormac Taylor Univ. of Dublin, Ireland

1993 James L. Madara Harvard Med. Sch. 2011 Sean P. Colgan University of Colorado Health Sciences CenterDavid Alpers Washington Univ. Linda C. Samuelson University of Michigan

Gary Wu University of Pennsylvania1995 Mark Donowitz Johns Hopkins Univ. Med. Sch.

Jeffrey Gordon Washington Univ. Med. Sch. 2013 Asma Nusrat Emory UniversityRobert Coffey Vanderbilt University

1997 Peter Traber Univ. of Pennsylvania Thaddeus Stappenbeck Washington UniversityW. James Nelson Stanford Univ.

1999 Kim Barrett Univ. of California, San DiegoDaniel Louvard Institute Curie, Paris

2001 Gail Hecht Univ. of Illinois, ChicagoJames Anderson Yale Univ. Sch. Of Med.

2003 James Goldenring Med. Col. Of GeorgiaJeffrey Matthews Univ. of Cincinnati Col. Of Med.

Gastrointestinal Tract XVI. GI HomeostasisPast Conference Organizers

Year# of

Applicants# of

Participants Commercial Non-

Commericial Government Total Raised1985 141 116 11,000.00$ 28,759.00$ 1987 124 96 14,000.00$ 40,000.00$ 1989 125 106 9,575.00$ 44,825.00$ 1991 193 160 10,000.00$ 75,550.00$ 1993 143 115 7,500.00$ 68,850.00$ 1995 107 95 14,000.00$ 64,065.00$ 1997 121 97 15,000.00$ 28,811.00$ 1999 73 70 20,000.00$ 83,800.00$ 2001 101 93 8,000.00$ 78,000.00$ 2003 82 80 12,000.00$ 54,644.00$ 2005 103 100 $64,600.00 5,000.00$ 15,000.00$ 84,600.00$ 2007 124 120 47,062.50$ 7,440.00$ 24,000.00$ 78,502.50$ 2009 183 180 46,000.00$ 10,000.00$ -$ 56,000.00$ 2011 154 150 20,000.00$ 10,000.00$ 22,000.00$ 52,000.00$ 2013 174 171 31,500.00$ 29,321.00$ 60,821.00$

ATTENDANCE FUNDING

Comparison of Previous Conferences

$17,759.00$26,000.00

Gastrointestinal Tract XVI. GI Homeostasis

$70,000.00$42,644.00

$35,250.00$65,550.00$61,350.00$50,065.00$13,811.00$63,800.00

December 16, 2011 Dr. Asma Nusrat Emory University School of Medicine Dept of Pathology & Laboratory Medicine Atlanta, GA USA Re Proposal #: 13-09 Dear Dr. Nusrat: We would like to thank you for submitting a proposal for the 2013 FASEB Summer Research Conference series. After careful consideration by the Advisory Committee, the proposal entitled, "Gastrointestinal Tract XV: Epithelia, Microbes, Inflammation and Cancer" has been approved. However the committee had the following suggestions:

The submitted proposal does not indicate which speakers have confirmed. Please specify which speakers have confirmed, making an effort to confirm all session chairs.

The proposal should have a more detailed plan for the Meet the

Expert session. Please note that this is a time for attendees (especially students/junior investigators) to meet and network with the “experts” of your program. The committee requests that you make every effort to include young investigators (poster presentation or a short talk) as early as possible within the conference agenda. This will have an enormous impact on one’s experience at the conference. This will also allow other participants to learn early on about their work and will then greatly increase interaction. We will soon begin the process of developing the conference schedule and will let you know the location and date of your conference as soon as this has been decided. Please keep in mind, location and date preferences are not guaranteed however we do our best to give you your first choice. In February, an Organizer Manual will be posted at our website (www.faseb.org/SRC) to assist you in your conference planning efforts. Please be aware that by agreeing to be an Organizer/Co-organizer of a FASEB Summer Research Conference, that should you decide to cancel the conference for any reason, you will be held responsible for any fees related to the cancellation (i.e., fees charged by the host location).

Dr. Nusrat Page 2 The Summer Research Conferences have been very successful over the years due to the commitment and dedication of the Organizers. On behalf of the Federation and the Summer Research Conferences Advisory Committee, your efforts in contributing to the success of the program are sincerely appreciated. A copy of this letter has also been sent to your co-organizer(s). Please do not hesitate to contact the SRC office by telephone at (301) 634-7010 or via the emails listed below with any questions. We look forward to working with you on this project over the next few years. Sincerely,

Jessica Lyons Conference Manager FASEB Summer Research Conferences jlyons@faseb.org

Emily Benson Conference Manager FASEB Summer Research Conferences ebenson@faseb.org

Robin Crawford Conference Manager FASEB Summer Research Conferences rcrawford@faseb.org