2011 24 phoenix dactylifera

13
1 24 2011 Phoenix dactylifera L polyphenol oxidase (ppo) ppo

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Page 1: 2011 24 Phoenix dactylifera

1

242011

Phoenix dactylifera

L

polyphenol oxidase (ppo)

ppo

Page 2: 2011 24 Phoenix dactylifera

2

Phoenix dactylifera L.Arecaceae

(13) Al-Hooti

% (20)Tafti and

FooladiMozafati

Shamsaei 1.7

Awad (16)

Page 3: 2011 24 Phoenix dactylifera

3

ppoEC : 1.14.18.1

whatman No.1

Folin- Denis Reagent

Page 4: 2011 24 Phoenix dactylifera

4

Standard Curve

ك باستخذاو طزٌقت فونن انشكم)1( انمنحنى انقٍاسً نتقذٌز حامض انتانٍ

y = 0.0394x

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

0 2 4 6 8 10 12

حامض انتانٍك)مهً مول(

76

0 ً

جمو

ل طو

ذ عن

ت صٍ

صامت

لاا

PH 7

PVP

PH 7

Substrate solution

spectrophotometer

Page 5: 2011 24 Phoenix dactylifera

5

xg

Factorial Experiments

23)

Page 6: 2011 24 Phoenix dactylifera

6

ppo

Tannins

, 1721,16

Page 7: 2011 24 Phoenix dactylifera

7

ppo

Sukkary ,

hilwa ,Khalas ,Sullaj14

18

ppo

تمز صنف انحلاوي خلال مزاحم فٍنونٍت نثمار نخٍم ان تغٍزاث فً انمواد ان انشكم )2( ان

تطورها

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

0.5

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32

اسبوع بعذ انتهقٍح

ولاٍننف

ز اكٍ

تز

انثمزة انبذرٌت

انثمزة انبكزٌت RLSD = 0.0991

Page 8: 2011 24 Phoenix dactylifera

8

ppo

Page 9: 2011 24 Phoenix dactylifera

9

انشكم )3( انتغٍزاث فً انفعانٍت الانزٌمٍت نهبونً فٍنول اوكسٍذٌز خلال مزاحم اننضج نثمار نخٍم انتمز صنف انحلاوي

0

50

100

150

200

250

300

0 2 4 6 8 10 12 14 16 18 20

اسبوع بعد التلقيح

مل /

ة حد

وت

ميزي

لان ايت

الفع

ال

الثمزة البذريت الثمزة البكزيت

RLSD = 19.06

Page 10: 2011 24 Phoenix dactylifera

11

.

phoenix dactylifera L.

Phoenix dactylifera L .

Page 11: 2011 24 Phoenix dactylifera

11

Phoenix dactylifera L

420

13- Al-Hooti , S. ; Sidhu , J. S. and Qabazard , H. ( 1997). Physico- chemical characteristics of five date fruit cultivars grown in

the United Arab Emirates . Plant Foods for Human Nutrition 50 : 101- 113.

14- Al-Qarni,S .S . (2005). Biochemical changes during date palm fruits ripening .Thesis – Department pf Biochemistry -

University , Saudi Arbic Kingdom .

15- Amiot, M.J. , Fluriet ,A., Cheyier, V., and Nicolas,J.(1997) . Phenolic compounds and vegetables .Cleandon Press,

Oxford,pp.51-85. 16- Awad , M. A. (2007). Increasing the rate of ripening of date

palm fruit (Phoenix dactylifera L.) cv. Helali by preharvest

and postharve treatments. Postharvest Biology and Technology 43 : 121 – 127.

17- Bacha , M. A. ; Nasr , A.T. and Shaheen, M .A .(1987). Changes in physical and chemical characteristics of the fruits of four

date palm cultivars .Proc. Saud . Biol. Sci. 10 : 285

18- Dowson,V.H. and Aten , A. (1962). Date handling ,processing

and packing FAO Agric .Development paper 72.

19- Mayer, A. M. (1987). Polyphenol oxidases in plants – recent progress. Phytochem., 26: 11-20.

20- Tafti , A. G. and Fooladi, M.H. (2005). Changes in physical and chemical characterasetic of Mozafati date fruit during

development . J. of Biolog . Sci ., 5 : 319 –322.

Page 12: 2011 24 Phoenix dactylifera

12

21- Tafti , A.G. and Fooladi, M.H. (2006). A study on the physico - chemical properties of Iranian shamasaei date at

different stages of maturity. World J . of Dairy and Food Sciences 1: 28 –32.

22- Walker, J. R. and Ferrar, P. H. (1998). Diphenol oxidases, enzyme- catalysed browning and plant disease resistance.

Biotechnol. Genet. Eng. Rev.,15: 457 - 498.

23- Westwood ,M.N.(1993). Temperate zone pomology , 3th

ed .Temper Press, Portland ,Oregon, 532p.

Page 13: 2011 24 Phoenix dactylifera

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Basrah . J.Agric.Sci., 24 (3)2011

Certain changes in phenolic compounds for date palm

(Phoenix dactylifera L.) of seeded and parthenocarpic fruits

(cv. Hillawi)

Muntaha Abdul-zahra Batool Hanoon Falih

Date palm research center Basrah university

SUMMARY

A study was conducted on changes in phenolic compounds for seeded and parthenocarpy date palm fruit c.v. Hillawi during different

growth stage ,The results showed that parthenocarpic fruits content of tannins was high in all stage except first stage of growth (at the eight week after pollination),tannins content 0.22% and 0.18% in parthenocarpic

and seeded fruits respectively . were seeded fruit enter log phase of growth the tannin content percentage increased and reached maximum value Kimri

stage of (0.22%) at 10th

week after pollination , Then the tannin content decreases till it reached the lowest value at in Tamer stage (18

th week after

pollination) , As for ,parthenocarpic fruit there was appeared significantly at log phase of growth (10

th week after pollination and reached maximum

value at 13th

weak after pollination for reach 0.44% ,then the tannin content declined rapidly and steady at 14

th until 15

th week after pollination ,then ,

decreased tannin content at entered it in Khalal stage and continue antil the Tamer stage (29

th week after pollination to reach ,the lowest value o.19%.

As for polyphenol oxdase Enzyme (ppo) appeared activity at eight week after pollination in both seeded and parthenocarpic fruits and high

significantly . Then ppo Enzyme gradually 13th

week after pollination (end Kimri and began Khalal stage ) , at enter seeded fruits Khalal stage ppo activity increased to reached their maximum value at 14

th week after

pollination , Then ppo activity gradually decreased till the entered seeded fruits tamer stage . As for , parthenocapic fruit there was self action for

seeded fruits in Enzyme activity beginning from 10th

week after pollination because delay growth parthenocapic fruit .