Nucleotide Metabolism

()

(RNA or DNA)RNADNA()()()

--------cAMP, cGMP----NADFADCoA---UDPG, CDP-DG, SAM ATP----GTP---- UTP----CTP----

() CO2() ,

Bases/Nucleosides/Nucleotides BaseBase + Sugar=NucleosideBase + Sugar + Phosphate=NucleotideAdenineDeoxyadenosineDeoxyadenosine 5-triphosphate(dATP)

()CO2Asp()(), ,CO2

R-5-PPRPP synthetasePP-1-R-5-P(PRPP)AMP

5-5-PR11IMPIMPCO25-IMPAMPGMP

IMP(11) 3 A:(1) B:(2-- 6) C:IMP(7--11) AMPGMP

AMP10

()(1) IMP(11)R-5-PAMPPRPPPP-1-R-5-P(PRPP)5-

PP-1-R-5-PGlnGlu3: H2N-1-R-5'-PGly3 ATP, Mg2+(GAR)PRA

FH4(GAR)HN=Glu(FGAR)(FGAM)

AIRATP,Mg2+,K+H2O(FGAM)5-(AIR)5--4-(CAIR)

Asp5--4-(CAIR)5--4-(N-)-(SAICAR)

5--4-(N-)-(SAICAR)5--4-(AICAR)

5--4-(AICAR)5--4-(FAICAR)N10FH4FH4

5--4-(FAICAR)H2O(IMP)

(2) IMPAMPSAsp, Mg2+,GTP

(AMPS)(AMP)

IMPNADH+H+XMPIMP

GlnGluXMPGMP

AMPADPATPGMPGDPGTP

::

----- PRPPKGPATATP5- PRPPIMP -----ATPGMP GTP GTPATP

6:AMPGMPIMPPRPPKGPAT2:AMPASSGMPIMPD

2. : 222

2IMPAMPSXMPAMPADPGMPGDPGTPATPATPGTP

Adenine phosphoribosyl transferaseAPRT) - (Hypoxanthine-guanine phosphoribosyl transferaseHGPRT) (Adenosine kinase)

()(1) PRPPPRPPPPiAPRTHGPRTAPRT

+1-+Pi(2) 1-, +ATP+ADP

------. 1. (.) 2. (.)

: (,)Lesch-Nyhan -,-----X:

() AMPGMPXMPAMPSIMP

() ()NADPH+H+NADP+ +H2OdNDPNDP

NDPdNDPNADP+NADPH+H+-(SH)2,Mg2+(FAD)

NDPdNDPADPdADPGDPdGDPUDPdUDPCDPdCDPTDPdTDP

dNDP+ATPdNTP+ADPdCDP+ATPdCTP+ADPdUDP+ATPdUTP+ADPdGDP+ATPdGTP+ADPdADP+ATPdATP +ADPdTTP?dNDPdNMP+Pi

, . , ()

1. :8-6-

AMPIGMPGX

() 3 (1) (2) 1-1- 5-5- (3)

PiNH3PiNH3

1-

NADP+NH3Pi1-NH3

0.12-0.36mmol/L : 0.27mmol/L:0.21mmol/L , 0.48mmol/L(8mg%), , (,

PRPP

CO2

1. 1 CO2

(2)

CO26 UMP

UMP

(3)

UMP

(4) TMP

III()()N-UMP()(CPS-I)(CPS-II)

() 1. (1) ----6HCO3_+Gln

+Asp

(OMP)(UMP)

ATP(2) (CTP)UDPUMPADPUTPGln, ATP Glu, ADP+Pi ATPADPCTP

dCMPdUDPPiNH3dUMPdTMPATPdTDPADPdTTPATPADPdTMP(3) (dTMPTMP)

(5) :ATP--- PRPP---

2 ---4A:CPS UMPB:(CAT) UMPCTPC:OPRT ADPGDPD:CTP(CTPS)CTP CTP,CTPCTP

2.

1 +PRPP UMP + Ppi

2

+1- +Pi+ATP Mg2+ UMP + ADP

2. ATP+CO2+GlnPRPPPRPPATP+ 5'-OMPUMPUTPCTP31CPS-II23PRPPAsp

() +PRPP+PPi+ATPUMP+ADP,TK

. 21R1132R21R13R1C222R2R14R15NADPH

1. 2. ,3. 2() 4. ,1. 5'-P -R2. IMP3. IMPAMPGMP1. 5'-P-R2. UMP3. UMP, CTP, dTMP

5'-P-RPRPPIMPdAMPGMPdGMPAMPdADPGDPdGDPADPdATPGTPdGTPATPUMPCMPdUMPUDPCDPdUDPUTPCTPdUTPdTMPdCMPdTDPdCDPdTTPdCTPCO2+GlnH2N-CO-POMPdCMP

. -----

1 PRPP x- HGPRT Lesch-Nyhan HGPRT x- ,, B, (ADA) (PNP) APRT 28- 2

() :5-

NH3NADP+H2OCO2+NH3H2N-CH2-CH2-COOH-

NADP+CO2+NH3--H2O

-

? A. B. C. D. E.

2. 5-A. DNAB. C. D. E.

3. A. B. C. D. E.

4. A. B. 6-C. 1-D. 1,6-E. 5-

5. A. B. C. D. E.

6. , ?A. B. C. D. E.

7. -CMPAMPTMPUMPIMP

, ?GTP-

9. PRPP, A. R-5-PPRPPB. C. PRPPD. IMPAMPE. IMPGMP

10. A. 5-B. FH4C. D. E.

11. The main tissue of de nove synthesis of purine nucleotide in vivo isA. thymus glandB. villous coat of small intestineC. liverD. spleenE. marrow

12. The main end product of purine nucleotide katabolic metabolism in human body isA. ureaB. creatineC. CreatinineD. uric acidE. -alanine

13. The methyl of thymine come fromN10-CHO FH4N5,N10=CH-FH4N5,N10-CH2-FH4N5-CH3FH4N5-CH=NHFH4

14. 6-mercapto-purine nucleotide doesnt suppressA. IMPAMPB. IMPGMPC. PRPP amide transferaseD. Purine phosphoribosyltransferaseE. Pyrimidine phosphoribosyltransferase

15. A B CO2C D E

16. PRPPA B C D NMPNDPNTP

17. A B C D

18. A B C

19. The compound which can produce feedback suppression of purine nucleotide synthesis is A IMPB AMPC GMPD uric acid

20. Which compound produce uric acid as its decomposed metabolism end product ?A AMPB UMPC IMPD TMP