ecm - main structural tissue of skin › helps skin renew and generate › provides signals to...
TRANSCRIPT
ECM - main structural tissue of skin› Helps skin renew and generate› Provides signals to intercellular pathways
Main components› Glycoproteins (such as collagen)› Proteoglycans› Hyaluronic Acid
Engineered ECMs are known as scaffolds
Ability to create scaffolds › Mimic the ECM in size and porosity› Have high surface to volume ratio
Easy to vary mechanical and biological properties through changing materials
Flexible- allows cells to manipulate their environment
Biocompatible polymer
Biodegradable at a slow enough rate to allow increased cell growth and stability
Easy to manipulate
Relatively low melting point - easy to use
Clinically safe (FDA approval)
Proven to have potential for scaffolds in relation to tissue regeneration› Has created scaffolds w/ ideal conditions
High porosities Large amounts of surface areas
Much research has shown that adding another biochemical can:› Increase stress resistance› Provide better adhesion of cells to the final scaffold› Increase the potential for cell proliferation
Biochemical should› Be a component of skin naturally› Must be able to be combined in a solution to be
electrospun
Natural polymer that exhibits biocompatible and biodegradable qualities
Cellular binding capabilities
Anti-bacterial properties
High viscosity which limits electrospinning
Good for health reasons (low toxicity, immunogenic)
Low cost – easily obtained
Poor spinnability - possibly fixed with addition of a synthetic polymer
By combining PCL with another biological polymer, an electrospun mesh can be created that mimics the ECM, exhibits optimal biocompatibility, and encourages cell attachment and proliferation
Baseline can be set for comparison of images› For the Second stage: microscope used› For the Third stage: cells used
Control image- image X
Triplicates created
Conclusion can be drawn based on rate of cell growth measured
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