thorlabs.com - cerna components: objectives and objective ......the cerna series mind map is a...

Thorlabs.com - Cerna Components: Objectives and Objective Holders

https://www.thorlabs.com/newgrouppage9_pf.cfm?guide=10&category_id=&objectgroup_id=8566[6/22/2016 9:23:11 AM]

CERNA COMPONENTS: OBJECTIVES AND OBJECTIVE HOLDERS

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Cerna Application SupportThorlabs has engineers, application specialists, anda sales team available to discuss the various Cerna options and towork with you to create a system that is optimized for your uniqueexperimental requirements. If you would like to be contacted by amember of our team, please let us know by [email protected].

Features

Objective HoldersNosepieces Hold One or Two Objectives in the MicroscopeSimultaneouslyCompatible with Dry, Water-Immersion, or Oil-Immersion ObjectivesDesigned for M25 x 0.75 or M32 x 0.75 Objective Threads (Nikon)Adapters for RMS (Olympus) and Other Thread Standards Available

ObjectivesSelection of Popular Objectives from NikonPlan Fluorite Objectives Provide Flat FOVs Corrected for Spherical and Chromatic AberrationsOther Objectives Available Upon Request

Objective holders for Cerna microscopes consist of two pieces: a nosepiece and a mounting arm. We currently offer options that enable you to attach eitherone or two objectives simultaneously. To make it easier to get started with your imaging setup, this page also contains a selection of widefield imagingobjectives commonly used with Cerna microscopes, although our microscopes are compatible with objectives from all major manufacturers.

Cerna Components Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7

Overview Microscope BodyWidefieldViewing

Epi-IlluminationObjectives and

Objective HoldersSample Holders Motion Control Trans-Illumination

O V E R V I E W

Nosepieces Hold Either One or Two ObjectivesPlan Fluorite, Infinity-Corrected Objectives from Nikon

► ►

N40X-PF40X Objective

MBP70100Dual-Objective Nosepiece

CSA1100Single-Objective Nosepiece

Cerna Rig Equipped withDual-Objective Nosepiece

ayangText BoxMBP70100 - June 22, 2016Item # MBP70100 was discontinued on June 22, 2016. For informational purposes, this is a copy of the website content at that time and is valid only for the stated product.



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CSA1100 NosepieceThis nosepiece is attached directly to the objective focusingmodule using the six indicated M4 counterbores.

Click for DetailsDrawing of CSA1100 Single-Objective Nosepiece

Drawing of MBP71100 Single-ObjectiveNosepiece

Drawing of MBP70100 Dual-ObjectiveNosepiece

MBP71100 and MBP70100 NosepiecesThese nosepieces are held by a dovetail mechanism and an M4 setscrew (2 mm hex) in the CSA1200 mounting arm, which provides the sixM4 counterbores needed to connect to the objective focusing module.

Click for DetailsDrawings of CSA1200 Mounting Arm

This tab gives the key mechanical features of our nosepieces and mounting arms, showing how these are connected to each other and to the microscope

body. For full details on mechanical features, including dimensions, click on the red Docs icon ( ) below.

D R A W I N G S

The Cerna Series Mind Map is a visual tool for selecting the modules that make up a complete Cerna microscope. Created as a supplement to the informationprovided directly on our website, it lays out both the required and optional components in a single 11" x 17" printed sheet. We have designed it to be used as aflowchart, starting from the red arrow at the center of the document and following the steps in order.

Click here or on the image below to download a printable PDF (6 MB). The objectives and objective holders sold on this page correspond to Steps 5 and 7 inthe mind map.

C E R N A M I N D M A P

Hide Drawings



Using the Slider of the MBP70100 Dual-Objective Nosepiece

Hide Objective Holders: Nosepieces and Mounting Arms

Objective Holders: Nosepieces and Mounting Arms

Our nosepieces let you simultaneously install either one or two objectives into theCerna microscope. The two-objective option (Item # MBP70100) uses a slider toeasily swap between objectives, as shown in the video to the right, allowing you to use a low-magnification objective to find a region of interest and a high-magnification objective to image.

These nosepieces contain either M25 x 0.75 or M32 x 0.75 threads. For compatibility with a greater range of objectives, we manufacture an extensive selection ofthread adapters that convert between M25 x 0.75 threads, M32 x 0.75 threads, RMS (0.800"-36) threads, or virtually any other standard. The M25 x 0.75 and M32x 0.75 thread standards are most commonly used by Nikon objectives, while RMS threads are most commonly used by Olympus objectives.

Required AccessoriesThe nosepiece is the device that holds the objective, while the mounting arm is the component that connects the nosepiece to the Cerna microscope body. Sinceeach component is sold separately, please refer to the Required Accessories rows in the tables below to ensure you order everything you need.

Compatibility with DIC ImagingIf you would like to perform DIC imaging, you should choose the MBP71100 or MBP70100 nosepiece. The CSA1100 nosepiece is not compatible with DIC imaging.

InstallationThe CSA1100 nosepiece is attached directly to the objective focusing module using six M4 counterbores. To install the MBP71100 or MBP70100 nosepiece, firstslide the dovetail on the top of the nosepiece into the mating dovetail on the CSA1200 mounting arm (sold separately), then lock the dovetails together using theincluded M4 setscrew (2 mm hex). The resulting assembly can be attached to the objective focusing module using the six M4 counterbores on the mounting arm.See the Drawings tab for details.

Options CSA1100 MBP71100 MBP70100

Number of Objectives One One Two

Nosepiece Photo(Click to Enlarge)

Objective Threads M32 x 0.75 M32 x 0.75 M25 x 0.75



Included Objective AdaptersM32 x 0.75 to M25 x 0.75 Adapter

M25 x 0.75 to RMS Adapter Nonea Nonea

Compatible with DIC Imaging No Yes Yes

Required Accessories None CSA1200 Mounting Arm CSA1200 Mounting Arm

Our M32A1 thread adapter converts M32 x 0.75 threads to M25 x 0.75 threads, our RMSA9 adapter converts M32 x 0.75 threads to RMS threads, and ourRMSA1 thread adapter converts M25 x 0.75 threads to RMS threads.

Part Number Description Price Availability

CSA1100 Single-Objective Nosepiece $170.27 Today

MBP71100 Nikon FN-MH Single-Objective Nosepiece $666.00 Lead Time

MBP70100 Nikon FN-S2N Dual-Objective Nosepiece $1,791.00 Today

CSA1200 Mounting Arm for MBP71100 and MBP70100 Nosepieces $194.73 Today

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Objectives

Shown below are selected widefield Nikon objectives that are commonly used with Cerna microscopes. We offer other objectives at our full web presentation, andcan also order other objectives from either Nikon or Olympus upon request.

Item # N4X-PF N10X-PF N20X-PF N40X-PF N60X-PF

Photo(Click to Enlarge)

Magnification 4X 10X 20X 40X 60X

Numerical Aperture (NA) 0.13 0.3 0.50 0.75a 0.85a

Working Distance (WD) 17.2 mm 16 mm 2.1 mm 0.66 mm 0.31 - 0.4 mm

Threading M25 x 0.75

If you would like to use trans-illumination to study slices, cell cultures, or other thin in vitro samples, note that the numerical aperture (NA) of yourcondenser should be larger than your objective NA, or the outer edge of the field of view will be noticeably dimmer than the center. This issue generallyonly arises with high-magnification objectives (40X or greater).

Part Number Description Price Availability

N4X-PF 4X Nikon Plan Fluorite Imaging Objective, 0.13 NA, 17.2 mm WD $454.74 Today

N10X-PF 10X Nikon Plan Fluorite Imaging Objective, 0.3 NA, 16 mm WD $826.32 Lead Time

N20X-PF 20X Nikon Plan Fluorite Imaging Objective, 0.50 NA, 2.1 mm WD $915.79 Lead Time

N40X-PF 40X Nikon Plan Fluorite Imaging Objective, 0.75 NA, 0.66 mm WD $1,043.16 Lead Time

N60X-PF 60X Nikon Plan Fluorite Imaging Objective, 0.85 NA, 0.31 - 0.4 mm WD $2,456.84 Lead Time

Visit the Cerna Components: Objectives and Objective Holders page for pricing and availability information:https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=8566

Cerna Series Microscopy Platform

www.thorlabs.com

Cerna Rig CRE1005With a platform for micromanipulators

and double camera port, this microscope combines confocal and electrophysiology capabilities on an

upright Cerna microscope.

Cerna Rig CRE1003This configuration is designed for electrophysiology experiments and includes rigid stands for micromanipulators.

Cerna Rig CRC1002This confocal microscope uses a Nikon dual camera port and fast XY Scanning stage.

Cerna Rig CRF1001The CSB1500F 500 mm Tall Microscope Body is designed to support setups for intrinsic signal imaging.

Cerna Rig CRW1003This widefield imaging microscope

includes a module for Dodt contrast imaging.

Cerna Rig CRF1002This intrinsic imaging rig uses microscope movers to translate the field of view over a

stationary sample.

Turret

ThorlabsNosepiece

Nikon Nosepiece

(Required for DIC in

Step 11)

Requires CSA1100, CSN1301, CSN1302, or MBP7110 from Step 5

WFA2001 Epi-Illuminator

CSA1200Sliding

Nosepiece Arm

CSA1100 Single

Objective Arm

CSA2100 SM2, 60 mm

Cage Arm

CSA1003Cerna 60 mm Cage to D1N Dovetail Adapter

Nikon MBP71100 Single Objective

Nosepiece

MCWHL5Cold White LED

M565L3Lime (565 nm) LED

MWWHL3Warm WhiteLED

M470L3Blue (470 nm) LED

Mounted High-Power LEDs (Only Compatible w/ WFA2001,

Other Wavelengths are Available)

LEDs

Lamps

HPLS243Solid State Plasma

Light Source

X-CITE200DC-Stabilized FluorescenceLight Source

X-CITE120LEDHigh-Power, Broad-Spectrum LED (Only Compatible w/ MBE74100)

SOLIS-1AHigh-Power LEDfor Microscopy,

6500 K (OnlyCompatible

w/ MBE74100)

CSA3000(/M)Breadboard Top

CSA3010(/M)Slim Breadboard Top

OPX2400(/M)Optical Path Conversion Kit

Nikon MBE74100 Epi-Illuminator

w/ Turret

Nikon MBP70100FN-S2N Sliding

2-Position Nosepiece

Nikon MBB74105Y-IDP Double Port

Nikon MBD75100FN-DP Variable MagnificationDouble Port

CSB1400400 mmTall Body

CSB1600600 mm

Tall Body

CSB1500F500 mm Tall

FunctionalImaging

Body

CSB1500500 mmTall Body

WidefieldIllumination

Used forDouble-CameraConfigurations

and Confocal Rig

Epi-IlluminationPaths

DIY Paths

Step 3:Double

CameraPort

Step 4:Epi-

IlluminationModule

Step 5:Objective

Holder

Step 6:Piezo Stage for Fast Z-Stacks

CSA1300Thorlabs SlidingNosepiece Arm

CSN1301Manual 2-Position

NosepieceCSN1302

Motorized 2-PositionNosepiece

CSA1400Turret Mounting Arm

MOT1500Manual 5-

Position TurretN4X-PF

*Other Upon

RequestN10X-PF N20X-PF N40X-PF N60X-PF

Step 7:Objective

MMP-2XYXY Mover forMicroscope

Body

PLS-XY1" XY Stage

PLS-X1" X-Axis Stage

CSB1350350 mm

Tall Body

Step 1:Body Height

Step 11:Condenser

Step 12:CondenserMounting Module

Step 14:DIC Components

Step 2:Widefield

Viewing Accessory

WithoutTrinoculars

SM2-SeriesThread

Nikon Trinoculars w/ Eyepieces & IR Filter (WFA4000) or w/out IR Filter (WFA4001)

WFA4107Nikon TI-3

Tube &C-MountAdapter

Nikon Erect Image Trinocw/ Eyepieces & IR Filter(WFA4002) or w/out IR

Filter (WFA4003)

WFA4108Nikon TI-3

Tube &0.7X C-Mount

Adapter

WFA4105Nikon Y-TV

Tube & C-Mount Adapter

1500M-GE1.4 MP Scientific Camera

4070M-GE4 MPScientific Camera

8050M-GE8 MPScientific Camera

340M-GEFast Frame Rate Scientific Camera

WFA4106Nikon Y-TV Tube & 0.7X

C-Mount Adapter

WFA41020.5XCamera Port

WFA4110Cerna Tube Lensin SM2 to D1N

Dovetail Adapter

WFA4111Cerna SM2 to D1N Dovetail

Adapter

WFA41010.75XCamera Port

WFA41001X Camera Port

PMP-2XY(/M)XY Manipulator

Platform

PMP1000(/M)Manual XY

Manipulator Platform

MCM30013-Axis Controllerfor CSB, PLS, and

ZFM Stages

MCM30023-Axis Controller

for MMP and PMP Stages

Step 10:Translation Stage

Step 9:Controller

Polarizers & Prismsfor DIC Condenser

DIC Objective Slider (Choose

One Compatible w/ Your Objective)

DIC CondenserPolarizers

DIC CondenserPrisms

Nikon MBD75300FN-PT Polarizer Turret for VIS & IR Polarizers

DIC Analyzer

Nikon MBN71950 FN-IR/FA IR &

VIS DIC Analyzer

Nikon MBN71946

FN-P Visible Rotatable

Nikon MBH76225DIC Slider 25X

Nikon MBH76240DIC Slider 40X Plan Fluor,

Plan APO (Air), Plan APO(LWD 40X 1.15 NA)


APO NIR (N2)

Nikon MBH76210DIC Slider 10X Plan Fluor


APO NIR (N2)

Nikon MBH76220DIC Slider 20X PlanFluor, Plan APO (N2 Dry)


Nikon MBN71960FN-IRP IR Rotatable

NikonMBH72300

N1 Dry (10X)

NikonMBH72310

N2 Dry(16X - 100X)

WFA3000DIC Turret Adapter

BSA2000Compact CondenserMounting Arm

CSA2000CondenserMounting Arm

CSA2100SM260 mmCage Arm

CSA2001Cerna SM2 to D3N Dovetail

Adapter

ZFM2020Condenser

Focus Module

ZFM2030Mid-Height Condenser

Focus Module

Nikon MBL78700D-CUO DIC Oil Condenser 1.4 NA

Nikon MBL78600D-CUD Universal Condenser 0.9 NA

Nikon MBL70100FN-C LWD Condenser 0.78 NA

LED Drivers

Illumination Kits

MountingArms

Brightfield Modulesand AccessoriesWFA1010

VIS LED

WFA1020NIR LED

WFA1050VIS & NIR LED

DC41004 Channels1 Mod Input

DC41044 Channels4 Mod Input

LEDD1B1 Channel

WFA1000Transmitted Light Only

WFA0150Transmitted LightModule Adapter

WFA1100Dodt/Gradient

Step 13:Transmitted Light Illumination

For Micromanipulators

CSA1000For MLS203-1

XY Stage

MZS500-EZ-Axis Piezo Stageand Controller Kit

CSA1001Manual Nikon XY Stage for Upright

Microscope

CSS2001Manual Olympus XY

Stage for Upright Microscope

CSA1050Clamp on Nikon NI-SS

Stage Adapter

CSS1051Clamp on Olympus

Stage AdapterMLS203-1

Fast XYScanning Stage

CSA1001SM1, 30 mm

Cage Arm

CSA1002SM2, 60 mm

Cage ArmFor Slides

For Recording Chambers

For MLS Inserts

Rigid Stand Sample Holders

Fixed Armsfor Microscope

Body

Step 8:Sample/Manipulator

Stages

Requires:• MBE74100 from Step 4• Nikon Nosepiece from Step 5• Brightfield Module from Step 13

PFM450E450 µm Travel

Piezo Stage

V 2 . 1

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