ZB-5ms GC ColumnsEnchanced Resolut ion of

Aromat ic Compounds

3

2.0

1

2

3 4 56

7 89 10

3.0 4.0 5.0 6.0 7.0 8.0 minLot 1Lot 2Lot 3Lot 4

REPRODUCIBLE RESULTSColumn: ZB-5msDimensions: 30 meter x 0.25 mm x 0.25 µm GC: HP 5890Carrier Gas: Hydrogen, 40 cm/sec @140 °CInjection: Split, 1:100, 250 °C, 1.4 µLOven Temp: 140 °C (isothermal)Detector: FID @ 325 °C

Sample:1. Decane2. 2-Ethylhexanoic Acid3. 1,6-Hexanediol4. 4-Chlorophenol5. Tridecane6. 1-Methylnaphthalene7. 1-Undecanol8. Tetradecane9. Dicyclohexylamine

10. Pentadecane

2

0.5

1.5

0 140 280 420 560 700Sample No.

Pea

k S

kew

2,4-Dichlorophenol

Hexachlorobutadiene

LONG LIFETIMEConsistent response after more than 700 samples at pH 2!

1

10 20 30 40 50 60 min

0.006

0.008

0.010

0.012

0.014

0.016

Volts

Passing Bleed Level

ms certified Bleedafter only 35 min!

Traditionally, GC/MS bleed certification had been achieved

by incorporating a column’s phenyl character into the

polymer backbone. These “Arylene phases” provided similar

selectivity to standard phases, but added the stability

necessary to reduce bleed. New bonding technologies have

allowed companies to produce “MS Certified” products

using non-Arylene chemistries, however, selectivity has not

always been equivalent.

Resolution differences are most commonly observed when

analyzing multi-ring aromatics such as PAHs and PCBs.

The high molecular weight and rigid bonding, allows certain

isomeric configuration to interact better than others.

The Zebron ZB-5ms is the accumulation of more than 15

years of GC column manufacturing experience! We have

applied our proprietary Engineered Self Cross-linking™ (ESC)

bonding to an Arylene polymer chemistry and created the

new Arylene Matrix Technology™. The resulting columns

have long lifetime, enhanced selectivity and lower bleed than

traditional Arylene products. Try one today and see what Ze-

bron’s new Arylene Matrix Technology can do for you!

ARYLENEMATRIX

TECHNOLOGY™

ENGINEERED SELF CROSS-LINKING™ (ESC)It starts with a careful fractionation of the base polymer resulting in very pure monomodal reactants. After the initial bonding, a second proprietary technique is applied which creates an extensively cross-linked ladder polymer network.

TraditionalArylenePhase

Arylene Matrix Technology™

ZB-5ms

Application of

ESCBonding

Fig. A

Fig. B

LOW BLEED / FAST CONDITIONINGAchieves MS certified bleed level after only 35 minutes! Little to no change in bleed after an additional 30 minutes of conditioning!

Sample Analyte Ret. Time (min) Ret. Time (min)

1. n-Nitrosoidimethylamine 3.82 2. Pyridine 3.85 3. 2-Fluorophenol 6.37 4. Benzaldehyde 8.28 5. Phenol-d5 8.45 6. Phenol 8.48 7. Aniline 8.72 8. bis(2-Chloroethyl)ether 8.72 9. 2-Chlorophenol-d4 8.75 10. 2-Chlorophenol 8.80 11. 1,3-Dichlorobenzene 9.17 12. 1,4-Dichlorobenzene-d4 9.30 13. 1,4-Dichlorobenzene 9.35 14. 1,2-Dichlorobenzene-d4 9.65 15. Benzyl alcohol 9.65 16. 1,2-Dichlorobenzene 9.70 17. 2-Methylphenol 9.92 18. Bis(2-chloroisopropyl)ether 10.02 19. Acetophenone 10.28 20. 4-Methylphenol 10.32 21. 3-Methylphenol 10.32 22. N-Nitroso-di-n-propylamine 10.33 23. Hexachloroethane 10.52 24. Nitrobenzene-d5 10.65 25. Nitrobenzene 10.70 26. Isophorone 11.32 27. 2-Nitrophenol 11.48 28. 2,4-Dimethylphenol 11.65 29. Bis(2-Chloroethoxy)methane 11.92 30. Benzoic Acid 12.00 31. 2,4-Dichlorophenol 12.08

EPA Method 8270C : Semivolatile Organic Analysis

OPTIMIZED RUN TIMES

Column: Standard 5msDimensions: 30 meter x 0.25 mm x 0.50 µmInjection: Splitless @ 250 °C, 1 µLCarrier Gas: Helium 64 cm/sec @ 50 °COven Program: 50 °C (hold 3 min) 50-325 °C @ 9 °C/min (Hold 2 min)Detector: MSD @ 300 °C

Column: Zebron ZB-5msDimensions: 30 meter x 0.25 mm x 0.50 µmPart No.: 7HG-G010-17Injection: Splitless @ 250 °C, 1 µLCarrier Gas: Helium 64 cm/sec @ 50 °COven Program: 50 °C (hold 3 min) 50-325 °C @ 9 °C/min (Hold 2 min)Detector: MSD @ 300 °C

vs. Zebron ZB-5ms NON-ARYLENE 5ms

Results in

‘‘Good Resolution of the PAH compounds Phenanthrene/Anthracene,

Benzo[a]Anthracene/Chrysene, and Benzo[b]fluoranthene/Benzo[k]fluoranthene considering we were able to shorten the run to an 18min run vs. our typical 29 min run.

Chris Hollingsworth, Test America, Analytical Testing Corporation

‘‘

Sample Analyte Ret. Time (min) Ret. Time (min)

63. 1,2-Diphenylhydrazine 18.98 64. 2,4,6-Tribromophenol 19.13 65. 4-Bromophenyl-phenylether 19.85 66. Hexachlorobenzene 19.95 67. Atrazine 20.40 68. Pentachlorophenol 20.48 69. Phenanthrene-d10 20.98 70. Phenanthrene 21.05 71. Anthracene 21.18 72. Carbazole 21.65 73. Di-n-butylphthalate 22.75 74. Fluoranthene 24.22 75. Benzidine 24.65 76. Pyrene 24.80 77. Terphenyl-d14 25.33 78. 3,3-Dimethylbenzidine 26.62 79. Butylbenzylphthalate 26.75 80. Benz[a]anthracene 28.02 81. 3,3-Dichlorobenzidine 28.03 82. Chrysene-d12 28.05 83. Chrysene 28.12 84. bis(2-Ethylhexyl)phthalate 28.37 85. Di-n-octylphthalate 30.03 86. Benzo[b]fluoranthene 30.72 87. Benzo[k]fluoranthene 30.78 88. Benzo[a]pyrene 31.47 89. Perylene-d12 31.60 90. Indeno[1,2,3-cd]pyrene 33.85 91. Dibenz[a,h]anthracene 33.90 92. Benzo[g,h,i]perylene 34.40

Sample Analyte Ret. Time (min) Ret. Time (min)

32. 1,2,4-Trichlorobenzene 12.32 33. Naphthalene-d8 12.45 34. Naphthalene 12.50 35. 4-Chloroaniline 12.67 36. Hexachlorobutadiene 12.85 37. Caprolactam 13.58 38. 4-Chloro-3-methylphenol 13.98 39. 2-Methylnaphthalene 14.32 40. Hexachlorocyclopentadiene 14.73 41. 2,4,6-Trichlorophenol 15.07 42. 2,4,5-Trichlorophenol 15.15 43. 2-Fluorobiphenyl 15.32 44. Biphenyl 15.57 45. 2-Chloronaphthalene 15.58 46. 2-Nitroaniline 15.88 47. Dimethylphthalate 16.45 48. 2,6-Dinitrotoluene 16.57 49. Acenaphthylene 16.67 50. 3-Nitroaniline 16.98 51. Acenaphthene-d10 17.05 52. Acenaphthene 17.13 53. 2,4-Dinitrophenol 17.25 54. 4-Nitrophenol 17.48 55. Dibenzofuran 17.60 56. 2,4-Dinitrotoluene 17.62 57. Diethylphthalate 18.35 58. Fluorene 18.50 59. 4-Chlorophenyl-phenylether 18.57 60. 4-Nitroaniline 18.62 61. 4,6-Dinitro-2-methylphenol 18.70 62. n-Nitrosodiphenylamine 18.88

3.283.326.05

8.178.188.128.278.338.378.678.758.809.209.229.239.589.58

9.95

9.939.9710.1810.2310.8511.0311.2511.4711.7511.67

11.8311.9211.9712.2312.47

13.5813.7714.3714.6214.7314.82

15.0215.4316.0516.2016.1016.5516.5216.6016.8317.1517.0217.2217.9317.9217.9818.1818.2818.33

18.4018.6219.2519.58

20.1020.3720.4320.5521.0322.2523.5824.0324.1524.70

26.1727.3527.4027.4027.4727.8329.3830.0830.1330.8230.9533.2233.2533.70

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Results in

I found the column to have exceptional resolution for

Benzo[b]fluoranthene and Benzo[k]fluoranthene. Paul McNamara, Severn Trent Labs - Buffalo

‘‘ ‘‘

Column: Zebron ZB-5msDimensions: 30 meter x 0.25 mm x 0.25 µmOrder No.: 7HG-G010-11Injection: Split 10:1 @ 250 °C, 1 µLCarrier Gas: Constant Flow Helium 1.4 mL/minOven Program: 40 °C for 0.5 min to 220 °C at 22 °C/min to 325 °C at 35 °C/min for 3 minDetector: MSD; 40-450 amu

12.5 13.5 min

107 108

109

110

111,112

2 4 6 8 10 12 14 min

APP ID 14953

15 min vs. 35 min

EPA Method 8270C Done in 15 minutes!

2 4 6 8 10 12 14 min

Sample Analyte Ret. Time (min)

1. N-Nitrosodimethylamine 2. Pyridine 3. 2-Picoline 4. N-Nitrosomethylethylamine 5. Methyl methanesulfonate 6. N-Nitrosodiethylamine 7. Ethyl methanesulfonate 8. Phenol 9. Aniline 10. Bis(2-chloroethyl)ether 11. 2-Chlorophenol 12. 1,3-Dichlorobenzene 13. 1,4-Dichlorobenzene 14. Benzyl Alcohol 15. 1,2-Dichlorobenzene 16. 2-Methylphenol 17. Bis(chloroisopropyl)ether 18. N-Nitrosypyrrolidone 19. 3-Methylphenol; 4-Methylphenol 20. N-Nitroso-di-n-propylamine 21. Acetophenone 22. N-Nitrosomorpholine 23. 2-Toluidine 24. Hexachloroethane 25. Nitrobenzene 26. N-Nitrosopiperidine 27. Isophorone 28. 2-Nitrophenol 29. 2,4-Dimethylphenol 30. Benzoic Acid 31. Bis(2-chloroethoxy)methane 32. 2,4-Dichlorophenol 33. 1,2,4-Trichlorobenzene 34. Naphthalene 35. a,a-Dimethylphenylamine 36. 4-Chloroaniline 37. 2,6-Dichlorophenol 38. Hexachloropropene

Sample Analyte Ret. Time (min)

39. Hexachloro-1,3-butadiene 40. N-Nitrosodibutylamine 41. 1,4-Phenylenediamine 42. 4-Chloro-3-methylphenol 43. Safrole 44. 1-Methylnaphthalene 45. 2-Methylnaphthalene 46. Hexachlorocyclopentadiene 47. 1,2,4,5-Tetrachlorobenzene 48. Isosafrole (isomer) 49. 2,4,6-Trichlorophenol 50. 2,4,5-Trichlorophenol 51. Isosafrole (isomer) 52. 2-Chloronaphthalene 53. 3-Nitroaniline 54. 1,4-Naphthoquinone 55. Dimethyl phthalate 56. 1,3-Dinitrobenzene 57. 2,6-Dinitrotoluene 58. Acenaphthylene 59. 2-Nitroaniline 60. Acenaphthene 61. 2,4-Dinitrophenol 62. 4-Nitrophenol 63. Pentachlorobenzene 64. 2,4-Dinitrotoluene 65. Dibenzofuran 66. 1-Naphthalenamine 67. 2,3,4,6-Tetrachlorophenol 68. 2-Naphthalenamine 69. Diethyl Phthalate 70. Fluorene 71. 2-Methyl-5-nitroaniline 72. 4-Chlorophenyl phenyl ether 73. 4-Nitroaniline 74. 4,6-Dinitro-2-methylphenol 75. Diphenylamine 76. Azobenzene

Sample Analyte Ret. Time (min)

77. 1,3,5-Trinitrobenzene 78. Phenacetin 79. 4-Bromophenyl phenyl ether 80. Hexachlorobenzene 81. Pentachlorophenol 82. 4-Aminobiphenyl 83. Pentachloronitrobenzene 84. Dinoseb 85. Phenanthrene 86. Anthracene 87. Dibutyl phthalate 88. 4-Nitroquinoline-n-oxide 89. Methapyriline 90. Isodrin 91. Fluoranthene 92. Benzidine 93. Pyrene 94. Aramite 95. Aramite (isomer) 96. Dimethylaminoazobenzene 97. Chlorobenzilate 98. 3,3-Dimethylbenzidine 99. Benzyl butyl phthalate 100. Kepone 101. 2-Acetylaminofluorene 102. 3,3-Dichlorobenzidine 103. Benz[a]anthracene 104. Bis(2-ethylhexyl) phthalate 105. Chrysene 106. Di-n-octyl phthalate 107. Benzo[b]fluoranthene 108. Benzo[k]fluoranthene 109. Benzo[a]pyrene 110. 3-Methylcholanthrene 111. Indeno[1,2,3-cd]pyrene 112. Dibenz[a,h]anthracene 113. Benzo[g,h,i]perylene

2.452.482.983.053.283.603.854.214.264.324.374.524.604.704.744.804.844.954.964.974.974.995.005.085.145.305.395.475.515.585.625.725.815.905.955.955.966.00

6.036.336.346.476.566.656.766.826.836.886.966.997.137.187.307.387.517.537.577.647.757.847.877.937.988.028.038.128.168.208.308.418.428.428.438.478.558.60

8.848.908.969.019.229.239.239.429.449.4910.0010.1810.2610.4210.5510.6710.7410.8610.9310.9911.0311.2711.2811.3111.4711.7011.7211.7411.7512.2412.6012.6312.9113.2414.1114.1314.45

The added resolution offered by the Arylene Matrix Technology™ allows run times to be shortened by at least 20-30 %.

www.phenomenex.com

8 10 12 14 16 18 20 22 min

Zebron ZB-5ms

17 min

11

12

1

2 3 45

6

78

910

1112

13

1415 16

11. Benzo[b]fluoranthene12. Benzo[k]fluoranthene

IMPROVED RESOLUTIONThe ZB-5ms Arylene Matrix Technology™ provides improved resolution of critical isomeric pair.

Dimensions: 30 meter x 0.25 mm x 0.25 µmInjection: Split 100:1 @ 250 °C, 0.5 µLCarrier Gas: Constant Pressure Hydrogen @ 1.2 mL/min (measured @ 140 °C)Oven Program: 40 °C (hold 2 min) to 250 °C @ 25 °C/min, to 265 °C @ 5 °C/min, to 300 °C @ 25 °C/min (hold until last peak elutes)Detector: FID @ 325 °CSample: EPA 610 mix @ 2,000 ppm

vs.

J&W® DB-5ms

Zebron™ ZB-5ms

J&W DB-5ms

17 min

11. Benzo[b]fluoranthene12. Benzo[k]fluoranthene

8 10 12 14 16 18 20 22 min

11

12

1

23 4

5 6

78

910

1112

13

1415 16

Almost

90 %ResolutionwithZebron™ ZB-5ms

84 %ResolutionwithJ&W® DB-5ms

Zebron™ ZB-5ms vs. J&W® DB-5ms

EPA Method 610: Polyaromatic Hydrocarbons (PAHs)

1. Naphthalene 2. Acenaphthylene 3. Acenaphthene 4. Fluorene 5. Phenanthrene 6. Anthracene 7. Fluoranthene 8. Pyrene 9. Benz[a]anthracene 10. Chrysene 11. Benzo[b]fluoranthene 12. Benzo[k]fluoranthene 13. Benzo[a]pyrene 14. Indeno[1,2,3-cd]anthracene 15. Dibenz[a,h]anthracene 16. Benzo[g,h,i]perylene

Conditions same for both columns

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ORDERING INFORMATION

Trademarks Zebron, Arylene Matrix Technology, and Engineered Self Cross-linking (ESC) are registered trademarks of Phenomenex, Inc.

Disclaimers J & W® is a registered trademark of Agilent® Technologies. All comparisons were done using new columns and similar testing conditions. Standard non-arylene 5ms product depicted is the Zebron™ ZB-5. Subject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/termsandconditions.

© 2008 Phenomenex. All rights reserved.

Zebron™ ZB-5msID (mm) df (µm) Temp. Limits (oC) Order No.

10-Meter0.10 0.10 -60 to 325/350 7CB-G010-02

15-Meter0.25 0.25 -60 to 325/350 7EG-G010-11

20-Meter0.18 0.18 -60 to 325/350 7FD-G010-080.18 0.32 -60 to 325/350 7FD-G010-510.18 0.36 -60 to 325/350 7FD-G010-53

30-Meter0.25 0.25 -60 to 325/350 7HG-G010-110.25 0.50 -60 to 325/350 7HG-G010-170.25 1.00 -60 to 325/350 7HG-G010-220.32 0.25 -60 to 325/350 7HM-G010-110.32 0.50 -60 to 325/350 7HM-G010-170.32 1.00 -60 to 325/350 7HM-G010-22

60-Meter0.25 0.25 -60 to 325/350 7KG-G010-110.32 0.25 -60 to 325/350 7KM-G010-11

Zebron™ ZB-5ms GUARDIAN™

ID (mm) df (µm) Temp. Limits (oC) Order No. 15-Meter with 10-Meter GUARDIAN™

0.25 0.25 -60 to 325/350 7EG-G010-11-GGC 30-Meter with 5-Meter GUARDIAN™

0.25 0.25 -60 to 325/350 7HG-G010-11-GGA0.25 0.50 -60 to 325/350 7HG-G010-17-GGA0.32 1.00 -60 to 325/350 7HM-G010-22-GGA

30-Meter with 10-Meter GUARDIAN™

0.25 0.25 -60 to 325/350 7HG-G010-11-GGC0.25 1.00 -60 to 325/350 7HG-G010-22-GGC

Test MixZebron ZB-5ms AG0-7578

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If Zebron does not provide you with equivalent separations as compared to any other GC column of the same phase and comparable dimensions, send in your comparative data within 45 days and keep the Zebron column for FREE!

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