Xspecies project

Wuhan 2011

Xspecies project

Xspecies project

R / BioConductor

training

AffylmGUItraining

Xspecies analysistraining

Normalisation, filtering and annotation

.CDF , filtering, stats and annotation

RMA Normalisation

Xspecies project

Hevea BrasiliensisRubber Tree

Chin Wei (Jonathan) Ong

Samples from Kew Gardens

Xspecies project

PxLyS1

PxLyS2 PxLyS3

RNAlater

Sampling strategyTwo plants (young / old)Three samples per leaf complex6 total chips.

Annotation: PxLySz

Sampling strategyTwo plants (young / old)Three samples per leaf complex6 total chips.

Annotation: PxLySz

cRNA

gDNA.cel

Xspecies CDF100

RNA.cel

Xspecies project

Chinese Purpleblow Maple (Acer truncatum)

Yuan Bao FengZhongxiu Zhang

Xspecies CDF150

Horqin Desert

Pilot Sampling strategy7 plants (6 month old) - 7 total chips.3 Beijing Cultivar3 Horquin Wildtype

1 drought challenged from Beijing Cultivar

Pilot Sampling strategy7 plants (6 month old) - 7 total chips.3 Beijing Cultivar3 Horquin Wildtype

1 drought challenged from Beijing Cultivar

Xspecies project

Saccharum sp.

Sugar CaneNatalia Martins

Xspecies CDF150

Sampling strategy3 stages x 3 replicates = 9 total chips

Sampling strategy3 stages x 3 replicates = 9 total chips

T1

T2

T3

Xspecies project

Mucuna pruriens var. utilis

VelvetbeanSandeepkumar Patil

Xspecies CDF100

Sampling strategyMany plants (3 month old)3 root samples3 shot samples= 6 total chips

Sampling strategyMany plants (3 month old)3 root samples3 shot samples= 6 total chips

Xspecies project

Remember to do the analysis

- and +

The cross species CDF

400

1000