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www.surrey.ac.uk
Manipulation of cells by dielectrophoresis – the effect of EHDBy Lionel Broche
With the help of Kai Hoettges
Biomedical Engineering Group
Supervisors: M.P.Hughes, S.Ogin, G.E.N.Kass
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Summary
Principles of experimentation with dielectrophoretic forces
Observations at low-frequency: the EHD effect
Presentation of the theory of EHD
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Manipulation of cells by dielectrophoresis – the effect of EHD
Principles of experimentation with dielectrophoretic forces
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Principles of experimentation with dielectrophoretic forces
Suspension medium
Membrane
Cytoplasmc, c
d, d
m, m
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Principles of experimentation with dielectrophoretic forces
E
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Principles of experimentation with dielectrophoretic forces
E
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Principles of experimentation with dielectrophoretic forces
E
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Principles of experimentation with dielectrophoretic forces
E
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Principles of experimentation with dielectrophoretic forces
E
FDEP
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Principles of experimentation with dielectrophoretic forces
E
FDEP
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Principles of experimentation with dielectrophoretic forces
Suspension medium
Membrane
Cytoplasmc, c
d, d
m, m
E
FDEP
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Principles of experimentation with dielectrophoretic forces
Suspension medium
m, m
E
2.r
Q -Q
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Principles of experimentation with dielectrophoretic forces
DEP theory for spherical particles:
FDEP = 4r3.m0.Re(K(
DEP force
Cell radiusDipole-related
factor
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Principles of experimentation with dielectrophoretic forces
1kHz 7kHz 50kHz 350kHz 2MHz 14MHz
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Principles of experimentation with dielectrophoretic forces
The DEP spectrum:
Frequency (Hz)
FDEP
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Principles of experimentation with dielectrophoretic forces
Electrode 1
Electrode 2
Gap
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Principles of experimentation with dielectrophoretic forces
The experiments give a measure of the DEP force for different frequencies of the signal
The result can be used in several ways (detection, measure…)
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Manipulation of cells by dielectrophoresis – the effect of EHD
Observations at low-frequency: the EHD effect
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Observations at low-frequency: the EHD effect
Low-frequency is <40kHz
Observation of parasitic flows
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Observations at low-frequency: the EHD effect
Electrode 1
Electrode 2
Gap
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Observations at low-frequency: the EHD effect
Electrode 1
Electrode 2
Gap
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Observations at low-frequency: the EHD effect
Electro-HydroDynamics: EHD
Caused by the electronic double-layer
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Manipulation of cells by dielectrophoresis – the effect of EHD
Presentation of the theory of EHD
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Presentation of the theory of EHD
Navier-Stokes equation:
gfvPvvt
vmEm
2.
Negligible since Re<<1
Pressure
Visquous forces
Electric forces
Gravitation
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Presentation of the theory of EHD
The electric force equation:
** .41Re21 EEEf qE
Coulomb forces Dielectric forces
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Presentation of the theory of EHD
The electric force equation:
** .41Re21 EEEf qE
Coulomb forces Dielectric forces
>>
EHD
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Presentation of the theory of EHD
The electric force equation:
** .41Re21 EEEf qE
Coulomb forces Dielectric forces
<<
Electro-thermal forces
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Presentation of the theory of EHD
ElectrodeSubstrate
Water
~nm
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Presentation of the theory of EHD
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Presentation of the theory of EHD
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Presentation of the theory of EHD
Enhanced DEP trapping
Fluid pumping
Others…
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Presentation of the theory of EHD
Thank you for your attention
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References
Castellanos, Ramos et al. (2003), “Electrohydrodynamics and dielectrophoresis in microsystems: Scaling laws”, Journal of Physics D: Applied Physics, 36(20): 2584
Hoettges, McDonnell et al. (2003), “Use of combined dielectrophoresis / electrohydrodynamics forces for biosensor enhancement”, Journal of Physics D: Applied Physics, 36(20): 101-104