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5 Foreword GABI Plant

6 Content 34 Management of germplasm and mapping10 Sitemap poulations - Construction of a high density12 Introduction transcript map of barley16 Patent and Licensing Agency (PLAfor GABI) Manoj Prasad, Nils Stein and Andreas Graner17 Business Platform promoting

GABI Plant Genome Research (WPG) 36 A core collection of barley cDNAsand expressed sequence tags (ESTs)

Resourcecenters and Bioinforrnatics Hangning Zhang, Nils Stein and Andreas Graner

The GABI resourcecenters KAT, GABI~lAPP, 38 Development of a DNA array based method forGABI Plant, GABI efficient identification of plant genetic resources

Konrad Bachmann, Frank Blattner,GABI-KAT J6rg Geistlinger, Dirk FischerSequencing of T-DNA flankings for "in silica"detection of tagged alleles (GABI-KAT - Kelner GABI TrafoArabidopsis T-DNA lines) 40 Overview: Qualitative and Quantitative

Improvement for Genetic Transformation of BarleyA Genoplante-GABI bi-Iateral project for sharingsequence data and Computer resources on T-DNA 42 Qualitative and Quantitative Improvement fortransformants of Arabidopsis thaliana Genetic Transformation of Barley (Subproject A)Bernd Weisshaar, Mario Rosso, Yong Li, Liliya Serazetdinova, Horst L6rz, Martina Brumm-ScholzSabine Steiner-Lange, Koen Dekker, Bernd Reiss,Heinz Saedler 44 Qualitative and Quantitative Improvement for

Genetic Transformation of Barley (Subproject B)GABI-lAPP Jochen Kumlehn, Rajiv Vishnoi, Gotz HenselGeneration of Arabidopsis and Barleyprotein expression libraries and chips 46 Qualitative and Quantitative Improvement forBirgit Kersten, Tanja Feilner, Armin Kramer, Genetic Transformation of Barley (Subproject C)Alexandra PoBling, Silke Wehrmeyer, Silke MOhlenbeck, Jens WeyenHans Lehrach, Dolores J. Cahill

Barley Transposcn2-D gel electrophoresis and mass spectrometer 48 Transposon-Mutated barley - A Powerful Toolfor the analysis of plant proteomes for Genome AnalysisJohan Gobom, Patrick Giavalisco, Niklas Gustavsson, Thomas KoprekEckhard Nordhoff, Joachim Klose, Hans Lehrach

GABI Info30 Identification of protein-protein interactions using 50 Bioinformatic Resourcecenter for

an automated yeast two hybrid system Plant Genome ResearchLukas Burkle, Marion Amende, Rita Fischer, Hans-Werner Mewes, Klaus MayerEckehard Kuhn, Uli Schneider, Hans Lehrach,Alexander Heyl GABI-PD

54 GABI Primary Database32 RNA Expression analysis Svenja Meyer, Axel Nagel, Steffen Schulze-Kremer

Wilfried Nietfelt, Stefanie Albrecht, Holger Eickhoff,Antje KrOger, Lajos Nyarsik, Hans Lehrach,CATMA consortium

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Dlcotyledones

Research on Arabidopsis

The GABI Arabidopsis Community(Genetic Diversity, Protein Community, Gauntlets)

Genetic DiversityCreation of Novel Genetic Variants of ArabidopsisRhonda Meyer, Carsten MOssig, Thomas Altmann

Large-scale SNP identification and analysisin the Arabidopsis genomeK. Schmid, Thomas Mitchell-Olds

Establishment of high-efficiency mapping toolsDieter Berqer', Otto Torjek, Thomas Altmann

Establishment of high-efficiency SNP-basedmapping tools and development of methodsfor genome-wide mutation detectionRalf Stracke, Thomas Rosleff Sorensen, Bernd Weisshaar

Ligation detection reaction - TaqMan procedurefor SNP detection on genomic DNAAleksey Soldatov, Tatiana Borodina, Thomas Altmann,Bernd Weisshaar, Thomas Mitchell-Olds

Protein CommunityGenomics, Expression and Membranes: Identificationand functional analysis of transport proteinsReinhard Kunze, Marcelo Desimone, Anja Schneider,Rainer Schwacke, Anja Schmidt, Giorgio Gonella,Eric van der Graaff, Ulf-Ingo FIOgge, Wolf B. Frommer

Transcription factor function search: How doindividual factors regulate agronomical importantprocesses in plants? (Subproject A)Isabell Witt, Maria InesZanor, Bernd Muller-Rober

Transcription factor function search: How doindividual factors regulate agronomically importantprocesses in plants? (Subproject B)Gieta Dewal, Martin Sagasser, IIlona Zimmermann,Bernd Weisshaar

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GauntletsCarbon and Nutrient Signalling: Test Systems,and Metabolite and Transcript ProfilesMark Stitt

Analysing cell wall biosynthesis to studyits role in biotic and abiotic stress reactionsNicolai Obel, Bjorn Usadel, Tze Siang Choo,Markus Pauly

Spatio/temporal analysis of gene expressionprofiles within single cells and specifictissuetypes of developing Arabidopsis plantsElke l.ieckfeldt', Ulrike Simon Rosin, Daniela Zoller,Berit Ebert, Julia Kehr, Joachim Fisahn

Searching for signals: stress-induced changesin Arabidopsis metabolite, peptide and proteinpatternsStephan Clemens, JOrgen Schmidt,Ludger Wessjohann, Dierk Scheel

RapeseedOverview: Genome Analysis in Rapeseed ­Genetic, physical and functional characterizationusing Arabidopsis resources (GABI-GARS)

GABI-GARSBiochemical investigations of seed developmentin rapeseed functional gene analysis(Brassica napus)Roland Baetzel, Wilfried Luhs, Wolfgang Friedt

A strategy to identify regulatory genes importantfor the regulation of metabolic pathways in seedsduring rapeseed developmentUte Heim, Michael Geiger, Karin Herbers

Development of intervarietal substitution lines,QTL mapping, and synteny analysisWolfgang Ecke, Rubens Marschalek, Heiko Becker

Seed colour in oilseed rape (Brassica napus):Classical genetics, QTL analysis and candidate genesfor map-based cloning and marker developmentAna Gloria Badani, Rod Snowdon, Roland Baetzel,Tina Lotz, Renate Horn, Wilfried l.uhs, Wolfgang Friedt

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98 Oilseed rape (Brassica napus L.) - Establishmentof Segregating Progenies and Field Evaluation 118 Overview: The GABI Potato Community

Christoph Ludecke. Heinrich Busch, Dieter Stelling, (GABI CONQUEST)

Konstanze RichterGABI CONQUEST

100 Development and phenotypic description of basic 120 Identification and characterization of genes

material for genome analysis of oilseed rape controlling quantitative agronomic characters

Lutz Brahm, Jutta B6hm in potato by a candidate gene approachCONQUEST (Genes CONtrolling QUantitativE traits

Sugar Beet of Solanum Tuberosum)

102 Research on Sugar Beet Christiane Gebhardt, Hans-Reinhard Hofferbert,EckartTacke, Jens Lubeck

104 Overview: The GABI Sugar Beet Community(GABI Beet, GABI BOLT, Sweet GABI)

122 Overview: The GABI Poplar Project (GABI-POP)

GABI Beet106 Genome analysis of sugar beet (Subproject A) GABI POP

Christian Jung, Uwe Hohmann, Maike Niemann, 124 Isolation of tree-specific genes and promotersThomas Schmidt, Tim Thurau, Hans-Werner Mewes, by a transposon tagging approachStephen Rudd, Georg BOttner, Bernward Marlander, Matthias Fladung, Frank Deutsch, Sandeep KumarLothar Frese

108 Genome analysis of sugar beet (Subproject B)Silke Mohring, Ralf-Schafer-Pregl, J6rg Wunder, 126 onThomas Rosleff-Sorensen Charlotte Bulich,ADIS (DNA core facility headed by Bernd Weisshaar),Katharina Schneider

110 Genome analysis of sugar beet (Subproject C) 128 Overview Barley Diversity: Development of newDagmar Kulesa, Andreas Polley, Susanne Reich, DNA marker systems and utilization of geneticCarina Kehling and Martin Ganal resources for Barley

112 Genome analysis of sugar beet (Subproject D) 130 AB-QTL analysis in barley: Detection of favorableGeorg Koch genes for quality and yield traits from barley wild

species and their introgression into the eliteGABI Bolt germplasm of cultivated barley by means of

114 Cloning of the bolting gene B from sugar beet DNA markersChristian Jung, Uwe Hohmann, Klaus Pillen, Maria v. Korff Schmising,Britta Schulz, Georg Koch Huajun Wang, Jens Leon

Sweet-GAB! 132 Development of single nucleotide polymorphism116 Crosslinking Structural and Functional Genomics (SNP) markers for Barley (Hordeum vulgare L.)

with Regard to Metabolism and Development in (Subproject A)Sugar Beet Frank Ordon, Gisela Neuhaus, Jorg Schondelmaier,Britta Schulz, Katharina Schneider Jens Weyen

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134 Development of single nucleotide polymorphism GABI(SNP) markers for Barley (Hordeum vulgare L.) 152 Overview: Assessment and Functional Analysis(Subproject B) of Agronomically Important Genome RegionsRaja Kota, Nils Stein and Andreas Graner in Hybrid Rye

136 Development of new DNA marker systemsand the use of genetic resources for Barley 154 Structural and Functional Comparison of DiseaseLudmilla Malysheva, Martin Ganal and Resistance Genes in Rye and Other PlantsMarion Roder Thomas Bringezu, Bernd Hackauf, Bodo Linz,

Peter WehlingGABI Seed

138 Overview GABI Seed: Functional genomics 156 Assessment and Functional Analysis of Agrono-of developing and germinating barley seeds mically Important Genome Regions in Hybrid Rye

and Assessment of Agronomically Important140 Functional Genomics of Barley Seed Development Genome Regions from Exotic Germplasm in

(Subproject A) Hybrid RyeWinfriede Weschke, Nese Sreenivasulu, Subproject 1: Bernd Hackauf, Stephen Rudd,Volodymyr Radchuk, Sabine Gubatz, Ulrich Wobus Peter Wehling; Subproject 2a: Zoran Susic, Thomas

Miedaner, Hartwig H. Geiger; Subprojects 2b, 2c:142 Functional genomics of germinating barley seeds Heinrich Wortmann, Jorg Schondelmaier, Peer Wilde,

(Subproject B) Viktor KorzunElena Potokina, Nils Stein, Andreas Graner

Wheat144 Functional Genomics of Malting Quality (Subproject C) 160 AB"QTL analysis in wheat: Detection of favorable

Markus Herz, Sabine Mlkolajewskl, genes for quality and yield traits from wheat wildGunther Schweizer species and their introgression into the elite germ"

plasm of cultivated wheat by means of DNA markersGABI Map Klaus Pillen, Antje Kunert, Anne Schubert, Jens Leon

146 Comparative sequencing of two regionsof the Barley and Rice genome MaizeRoberto Cossu, Dragan Perovic, Nils Stein, 162 Overview: GABI Maize ProjectsMartin Ganal. Andreas Graner and Marion Roder (GABI Cool, Maize Promoter)

GABI Nonhost GABI Cool148 A Consortium-Based Functional Genomics Initiative 164 Genomics of Chilling Tolerance in Maize

on Plant Nonhost Disease Resistance Thomas Presterl, Karin Ernst, Evelyn M. Moller/Jane Parker/ Paul Schulze-Lefert, Patrick Schweizer, Dierk Milena Ouzunova, Hartwig H. Geiger, Peter WesthoffScheel, Thorsten Nurnberger, Markus Frank

PromoterGABI Agrotec 166 Isolation and analysis of monocotyledonous

150 Functional Analysis of the Barley Genome: promoters from Zea mays L.Functional Characterization of Agronomically Rene Lorbiecke, Katja Muller, Carina Paul,Relevant Genes and their Use to Improve Disease Reinhold Brettschneider, Gunter Donn,Resistance to the Genus Fusarium Udo WienandCarin Janssen, Sibylle von ROden, Rebekka Fensch,Karl-Heinz Kogel, Sophia Biemelt, Jochen Kumlehn, 168 Authors IndexUwe Sonnewald, Angelika Felk, Simon Henning, 170 GABI PublicationsJoe-Gmem Ekanem, Wilhelm Schafer 180 GABI Contacts

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