write the complementary dna strand for each sequence. 1.tacggcaaacttact 2. tacgccatt 3....
TRANSCRIPT
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Write the COMPLEMENTARY DNA strand for each sequence.
1. TACGGCAAACTTACT
2. TACGCCATT
3. TACAAAAAGTGGATC
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DNA Replication Review
• Flash Tutorial
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Learning Targets“I Can. . .”
-List three ways that RNA is different from DNA.
-List the three types of RNA.
-Describe the role of mRNA and tRNA during protein synthesis.
-Define “transcription” and where it occurs in the eukaryotic cell.
-Define “translation” and where it occurs in the eukaryotic cell.
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Protein Synthesis
DNA RNA Protein
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What is RNA? Ribonucleic Acid
1. Nucleotides
A G C U
ADENINE GUANINE CYTOSINE URACIL
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What is RNA? Ribonucleic Acid
2. Contains the sugar ribose, rather than deoxyribose
3. Is single stranded, rather than double stranded
4. Three types:a. mRNA (Messenger)
b. tRNA (Transfer)
c. rRNA (Ribosomal)
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Why do we have protein in our bodies?
1. Enzymes
2. Major component of the cell membrane
3. Structural component of skin and muscle
4. Basically, DNA codes for protein, which become you!
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Recall: DNA is made of nucleotides strung together into a chain. These
nucleotides code for amino acids.
1. Amino acids: the subunits strung together in a protein chain
2. The four nucleotides are grouped into threes: these groups of three are called codons.
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Recall: DNA is made of nucleotides strung together into a chain. These nucleotides
code for amino acids. 3. Each codon specifies a particular amino acid
that is to be placed in the protein PAGE 367
AAA = _________________
GGA = _________________
AUG = _________________
UAA = _________________
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DNA RNA (Transcription)
1. Transcription: a molecule of DNA is copied into an opposite strand of mRNA
*Transcription occurs because DNA cannot leave the nucleus, and proteins are made outside the nucleus
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DNA RNA (Transcription)
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DNA RNA (Transcription)
2. Steps:
a. DNA strands are separated by an enzyme
b. An mRNA strand is created from the DNA strand to be copied (remember base-pairing rules except . . . )
*G with C
*A with U
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DNA RNA (Transcription)
DNA = TACCGTTGCAACATT
mRNA =
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RNA Protein (Translation)
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RNA Protein (Translation)
1. Translation: the creation of a chain of amino acids from mRNA
mRNA = AUGGCAACGUUGUAA
Amino acids =
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RNA Protein (Translation)
2. Translation requires tRNA, which carries amino acids and rRNA, which makes ribosomes
3. Ribosomes floating in the cytoplasm and attached to the ER are the location of protein translation
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Alleles for diseases come from mutations
Mutations: mistakes made during DNA replication or protein synthesis1. Nucleotide substitution: TATGCA becomes TATTCA2. Nucleotide addition: TATGCA becomes TATGGCA3. Nucleotide deletion: TATGCA becomes TATCA
All produce frameshift mutations!
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RNA Protein (Translation)
1. TACGGCAAACTTACT
DNA
mRNA
Protein
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RNA Protein (Translation)
2. TACGCCATT
DNA
mRNA
Protein
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RNA Protein (Translation)
3. TACAAAAAGTGGATC
DNA
mRNA
Protein
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References
• http://www.safarikscience.org/biologyhome/7_dna/codon_question.png
• http://www.accessexcellence.org/AE/AEPC/WWC/1994/codon_bingo.php