8/3/2019 Worksafe Blood Culture Collection Procedure 1broch

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1. Skin PreparationPDI® Chlorascrub™ Swabstick

• Afterlocationofthevein,vigorouslyscrubthevenipuncture  sitewithPDI®Chlorascrub™Swabstickfor30seconds.1

• Allowthesitetoairdrybeforevenipuncture.

•Do not re-palpate the vein.

2.BottlePreparation • Inspecteachbloodculturebottlebeforeusetoensureintegrity 

ofbottleandsensoronbottomofbottleisintact.Thesensor  isnormallyauniformgrayish-greencolorandayellowcolor  wouldindicatecontaminationofthebroth.Discardanybottle  foundtobedamagedorwithasensorthatisyellow.

• Removeprotectiveiptopovercap.

•NOTE:Theseptumisnotsterileandmustbedisinfected.

• Cleansetheseptumwith70%alcohol.

•Thebottlehasbeenpre-markedwith5mlincrements.  Markthedesiredllvolumelevelonthebottlefor10ml.

3.VenipunctureandBottleInoculation  Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit

• Collectallnecessarymaterialsforadirectdraw:

from diagnosis,the seeds of better health

BacT/ALERT®

Blood Culture Collection Procedure

Availability WorkSafeBloodCultureCollectionKit 279102-279105*

Saf-THolder®BloodCultureDevice(AdaptorCapandInsert) 100/case 96004**

  *seebioMérieuxcatalogue

  **seeSmithsMedicalcatalogue

bioMérieux, Inc.100RodolpheStreetDurham,NC27712U.S.A.Tel: (800)682-2666Fax: (800)968-9494

bioMérieux Canada, Inc.7815Henri-BourassaBoulevardOuestSt.Laurent(Québec)Canada H4S 1P7Tel:(514)336-7321Fax:(514)807-0015

1-800-361-7321ext.221 www.biomérieux-usa.com

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Tips ’n’ Hints•Recommendedbloodtobrothratiois1:5to1:10.Asthevolumeofblooddrawnisincreased,theyieldofpositiveculturesincreases.Optimally,20mlofbloodshouldbedrawnfromadults(10mlperbottle).

•Whenlabelingthebottles,donotcoverthe peel-offsectionofthebarcodelabelsorthe lotnumbers.

•Orientadditionalpatientlabelverticallyfor scanningefciency.

•Forbestvolumecontrol,marklllevelonsideofbottlepriortocollection.

•Donotoverllthebottles,asthismaycause falsepositivereadings.

•WhenusingtheSaf-THolder®BloodCultureDevice(AdaptorCapandInsert),besureluerisconnectedrmlyandtheneedleisstraightwhenenteringandleavingtheseptum.Twistingorturningthebottlemayincreasethechancethatthesheathmaynotretractandreseal.

•Toavoidcontaminationofthebloodculturesample,inoculatebloodculturebottlesrst. Thenlladditionalbloodcollectiontubes.

Septicemiaoccursinapproximately200,000patientseachyear,andbetween30and50%ofthosepatientsdie.Becausethe consequencesofsepticemiaaresoserious,rapidandaccuratediagnosisofthisdisorderiscritical.

TheBloodCultureisthebestdiagnostictoolfordetectingsepticemia,andit’sabsolutelyimperativethatthebloodspecimenbecollectedproperlytoprovideclinicallyvaluableresults. BacT/ALERT®3Dinstrumentsareautomatedsystemsthatincubate,shakeandmonitorculturebottlesforsignsofmicrobialgrowth. SamplesfrompatientssuspectedforsepticemiaareinoculatedintoBacT/ALERTculturebottlesformonitoring.Thebottlesarecolor codedforeasyidenticationofmediatypeandaremadeofplasticforaddedsafety.

Performvenipuncture. Whentheneedleisinthevein,secureitwith

tapeorholditinplace.

2.

PlaceAdaptercapontheaerobic(blueorgreenlabel)BacT/ALERTculturebottle

septumandpressdowntopenetrateandobtainbloodow.HoldtheAdapterCapdownon thebottle.

3.

E CI E G

F RZ E REFRIGERTE

R T E P ER T U RE

I H

Usingthellindicatorlinesonthelabel,obtain8-10mlofblood.Move

theAdapterCapfromtheaerobicbottletotheanaerobic(purpleororangelabel)bottle(ifrequired)andcontinuethecollection.

4.

E CI E G

F RZ E RE F RI GE RTE

R T E P ER T U RE

I H

Ifadditionalbloodisrequiredforothertests,placetheAdapterInsert

intotheAdapterCapandsnapintoplace.Thismakesthecapcompatiblewithvacuumcollectiontubes.

5.

 Whennaltubeislled,removetourniquet.Terminatethe

venipuncture,activatethesafetyshield,grabthewingwithonehandandslidethewingsbackuntilitlocksinplace.

6. E CI E G

F RZ E RE F RIGE RTE

R T E P ER T U RE

I H

Disposeofwing andadaptercapin asharpscontainer.

7. SPECIMENBAG

F RO ZE N RE F RIGE RA TE

ROOM TEMPERATURE

BIOHAZARD

Labelspecimensandplaceinplasticbiohazardziplockbag.Bottlescannow

besafelytransportedviapneumatictubesystem.

8.

OR

GatherTourniquet,BacT/ALERT®bottles, Saf-THolder®Adapter,wing,Chlorascrub™Swabstick,gauze,alcoholpreppads.

1perbioMérieux/Cumitechguidelinesasthemanufacturercannotmakethispreptimerecommendation

1a.

OpentheWorkSafeBloodCultureCollectionKitwhichcontainsallthenecessarymaterialsinasingle,convenientpackage.

1b.