Winning Westerns—great resultsPremier products for Western blotting

10_2673 KE/KL ??-IW-4/2010 BN???????

For trademark ownership, see www.fishersci.com/trademarks.

Your Optimized Western Blotting Products Portfolio

One of the greatest challenges in advancing research is obtaining consistent, quality results. In Western blotting, several factors contribute to the success of experiments, including the quality of the protein samples, transfer efficiency of the proteins to the membrane and the reagents used throughout the protocol. Millipore® and Hoefer® offer an extensive array of Electrophoresis and Western blotting products that are optimized to work synergistically, providing strong specific signals and low background to help you quickly produce reproducible publication-quality results.

Description Size* Qty. Cat. No.

Immobilon P: PVDF 0.45μm 26.5cm x 3.75m 1 Roll IPVH00010

Immobilon PSQ: PVDF 0.2μm 26.5cm x 3.75m 1 Roll ISEQ00010

Immobilon FL: PVDF 0.45μm 26.5cm x 3.75m 1 Roll IPFL00010* Other sizes available

Hoefer® Nitrocellulose Membranes Ideal for Western blotting and other immunoblotting techniques

• Superior strength—withstand aggressive handling or can be used with automated equipment without breaking or tearing • Membrane binds a wide range of fragment sizes and can still be easily blocked for low background • Uniform pore structure provides consistent flow and diffusion rates

Description Qty. Cat. No.

0.22μm pore size 30cm x 3m 1 Roll 03-500-600

0.45μm pore size 30cm x 3m 1 Roll 03-500-601

0.45μm pore size 8cm x 9.5cm 10 Pk. 03-500-604

0.45μm pore size 16cm x 16cm 10 Pk. 03-500-602

0.45μm pore size 23.5cm x 20cm 10 Pk. 03-500-603

Millipore Immobilon™ Western Blotting Transfer Membranes Millipore offers three varieties of PVDF transfer membranes, each optimized for different protein blotting applications. All have exceptional handling characteristics and are compatible with a variety of detection chemistries.

• Excellent protein retention, physical strength and broad chemical compatibility• Compatibility with a variety of staining applications and reprobing techniques

Description Detection Method Qty. Cat. No.

Bløk-CH Reagent Chemiluminescence 500mL/bottle WBAVDCH01

Bløk-FL Reagent Fluorescence 500mL/bottle WBAVDFL01

Bløk-PO Reagent Phosphoprotein 500mL/bottle WBAVDP001

Millipore Bløk™ Noise-Cancelling ReagentsBløk reagents are a family of uniquely formulated inorganic blocking solutions that provide numerous benefits including:

• Reduced background for chemiluminescent or fluorescent detection• Pre-diluted synthetic reagents that are ready to use and stable at room temperature• Unique formulation for detection of phosphorylated proteins

Ordering Information:

Description Cat. No.

Mini Blotting Package with Semi-Dry Unit 03-500-805

Ordering Information:

Description Cat. No.

Mini Transfer Tank Combination Package 03-500-804

Package Includes:

Description Cat. No.

Hoefer Mighty Small™ Mini Deluxe Vertical Unit

03-500-111

Semi-Dry Transfer Unit 03-500-612

300V, 500mA, 90W Power Supply PS300BNA

Package Includes:

Description Cat. No.

Hoefer Mighty Small™ Mini Deluxe Vertical Unit

03-500-111

TE 22 Transfer Tank 03-500-216

300V, 500mA, 90W Power Supply PS300BNA

Your Optimized Western Blotting Products Portfolio

Save Time and Money with Complete Mini Western Blotting Packages*

Millipore Luminata™ HRP Chemiluminescence Detection Reagents The new family of Luminata HRP chemiluminescent substrates is premixed for convenience. Just pour directly onto the Western membrane without worrying about a pipetting error.

• Premixed for convenience to avoid cross-contamination of the bottles and stable at room temperature• High signal intensity and low background to enable detection of low protein concentrations• Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents

Description Qty. Cat. No.

Luminata Classico HRP Chemiluminescence Detection Reagent

100mL WBLUC0100

500mL WBLUC0500

Luminata Crescendo HRP Chemiluminescence Detection Reagent

100mL WBLUR0100

500mL WBLUR0500

Luminata Forte HRP Chemiluminescence Detection Reagent

100mL WBLUF0100

500mL WBLUF0500

Description Qty. Cat. No.

SNAP i.d. Protein Detection System WBAVDBASE

SNAP i.d. Consumables and Accessories

Single Blot Holder 30/Pk. WBAVDBH01

Double Blot Holder 30/Pk. WBAVDBH02

Triple Blot Holder 20/Pk. WBAVDBH03

Antibody Collection Tray 20/Pk. WBAVDABTR

SNAP i.d. Blot Roller 1/Pk. WBAVDROLL

Millipore SNAP i.d.™ Protein Detection System Rapid immunodetection in just 22 minutes!The SNAP i.d. Protein Detection system incorporates a vacuum that actively drives reagents through the blotting membrane.

• Fast immunodetection enabling more experiments in less time• Superior washes for a lower background• Equivalent or better signal to noise ratios than traditional methods

Hoefer TE70XP Semi-Dry Transfer UnitPerform uniform transfers of proteins from polyacrylamide gels in less than one hour • Built-in intelligent power supply prevents blots from dehydrating• Can run up to four mini gels at once for higher throughput• Easy operation and cleanup

Hoefer TE22 Mighty Small Transfer TankSafe, economical unit requires minimal current and minimal buffer • Built-in alumina heat exchanger permits efficient transfers in less than one hour • Platinum electrode ensures uniform electrical field for complete and even transfers • Hinged, color-coded cassettes enable simple loading and easy orientation of gel sandwich

Hoefer 300V, 500mA, 90W Power SupplySmall footprint • 90 watts, 500mA and 300 volts enables it to run four mini tanks at once• Low volt and amperage detection limits (can transfer a low amperage or voltage)• Large LED screen for easy viewing

Hoefer SE260 Mighty Small Mini Deluxe Vertical Unit Combine the advantages of a small format and efficient cooling for rapid screening of protein.Ideal for protein separation in 1-D and second dimension 2-D gels• Fits all 10x10cm precast gels• Built-in cooling for faster runs and sharper bands• Leakproof casting with the SE245 casting system

*Larger formats available