WHITE BLOOD WHITE BLOOD

CELL COUNTCELL COUNT

WHITE BLOOD WHITE BLOOD

CELL COUNTCELL COUNT

Functions of WBC:Functions of WBC:

a.Mainly functions to protect against disease.

b.Works mainly outside the blood stream

c.Diapedesis - squeezing through blood vessels

LeukocytosisLeukocytosis::WBCWBC above 10,000/mm3

Infectionacute appendicitis.

LeukopeniaLeukopenia::WBCWBC below 4,000/mm3radiationdrugs

Number of white blood cells Number of white blood cells per micro liter per micro liter (mm(mm33)) of Blood: of Blood: 4000 to 10,0004000 to 10,000

Marcano:Acetic acid lyses RBCMetilen blue coloring WBC

Method

0.1 mm depth Cover slip

Ruled area

V slash Moat

Neubaure hemocytometer lam

Neubauer hemocytometer lam

WBC Count

WW BC BC CountCount CalculatiCalculationon

N= (n1+n2+n3+n4)/4 × volume factor ×

dilution factor

volume factor = 10

dilution factor = 20